EY710860

Overview
NameEY710860
Unique NameEY710860
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length915
Libraries
Library NameType
Sweet orange fruit, development stadium (3 of 6)cdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of EY710860 vs. ExPASy Swiss-Prot
Match: MAO1_CITK8 (NAD-dependent malic enzyme OS=Citrobacter koseri (strain ATCC BAA-895 / CDC 4225-83 / SGSC4696) GN=sfcA PE=3 SV=1)

HSP 1 Score: 167.162 bits (422), Expect = 1.662e-41
Identity = 82/178 (46.07%), Postives = 125/178 (70.22%), Query Frame = 2 
Query:  194 SGYCLLRDPRHNKGLAFTEKERDAHYLRGLLPSSVISQQLQEKKLMISIRQYEVALQKYVAMMELEERNERLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKILEVLKNWPERSIQVIVVTDGERILGLGDLGCQGMGIPGGKLALYTALGGIRPSTCLPI 727
            +G  LL  P  NKG AF+ +ER    L GLLP  V S + Q ++  I  + ++  + K++ +  +++ NE LFY+L+ ++++E++PV+YTPTVG AC+++  I+RR +G++IS + +  + ++L+N P  +I+VIVVTDGERILGLGD G  GMGIP GKL+LYTA GGI P+  LP+
Sbjct:   16 AGPVLLEFPLLNKGSAFSIEERRNFNLLGLLPEVVESIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVQNHLDEMMPVIYTPTVGAACERFSEIYRRARGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERILGLGDQGIGGMGIPIGKLSLYTACGGISPAYTLPV 193

HSP 2 Score: 24.2534 bits (51), Expect = 1.662e-41
Identity = 9/15 (60.00%), Postives = 11/15 (73.33%), Query Frame = 3 
Query:  738 GDNNEQLLEDDFYIG 782
            G NN+QLL D  Y+G
Sbjct:  198 GTNNQQLLNDPLYMG 212
[Close]
BLAST of EY710860 vs. ExPASy Swiss-Prot
Match: MAO1_PECCP (NAD-dependent malic enzyme OS=Pectobacterium carotovorum subsp. carotovorum (strain PC1) GN=sfcA PE=3 SV=1)

HSP 1 Score: 169.859 bits (429), Expect = 1.869e-41
Identity = 84/178 (47.19%), Postives = 126/178 (70.79%), Query Frame = 2 
Query:  194 SGYCLLRDPRHNKGLAFTEKERDAHYLRGLLPSSVISQQLQEKKLMISIRQYEVALQKYVAMMELEERNERLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKILEVLKNWPERSIQVIVVTDGERILGLGDLGCQGMGIPGGKLALYTALGGIRPSTCLPI 727
            +G  LL  P  NKG AFTE+ER    L GLLP +V + + Q ++     ++++  ++K+V +  +++ NE LFY+LL  ++ E++P++YTPTVGEAC+ +  I+RR +GL+IS   +  I ++L+N  +++++VIVVTDGERILGLGD G  GMGIP GKL+LYTA GGI P+  LP+
Sbjct:   16 AGPILLEFPLLNKGSAFTEEERANFNLHGLLPEAVETIEEQAERAWRQYQEFKHDIEKHVYLRNIQDTNETLFYRLLDGHLSEMMPIIYTPTVGEACEHFSDIYRRARGLFISYPNRAHIDDMLQNATKQNVKVIVVTDGERILGLGDQGIGGMGIPIGKLSLYTACGGISPAYTLPV 193
[Close]
BLAST of EY710860 vs. ExPASy Swiss-Prot
Match: MAO1_SODGM (NAD-dependent malic enzyme OS=Sodalis glossinidius (strain morsitans) GN=sfcA PE=3 SV=1)

HSP 1 Score: 166.777 bits (421), Expect = 2.165e-41
Identity = 81/178 (45.51%), Postives = 125/178 (70.22%), Query Frame = 2 
Query:  194 SGYCLLRDPRHNKGLAFTEKERDAHYLRGLLPSSVISQQLQEKKLMISIRQYEVALQKYVAMMELEERNERLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKILEVLKNWPERSIQVIVVTDGERILGLGDLGCQGMGIPGGKLALYTALGGIRPSTCLPI 727
            +G  LL  P  NKG AF+ +ERD   L+GLLP +V + + Q ++     + +   + +++ +  +++ NE LFY+LL  ++ E+LP++YTPTVG+AC+++  I+RR +G++IS     KI ++L+N  +++++VIVVTDGERILGLGD G  GMGIP GKL+LYTA GGI P+  LP+
Sbjct:   16 AGPILLEFPLLNKGSAFSLEERDNFNLQGLLPDTVETIEEQAERAWRQYQDFRTNIDRHIYLRNIQDTNETLFYRLLAAHLAEMLPIIYTPTVGDACERFSDIYRRARGVFISYNNCDKIEDMLQNATKQNVKVIVVTDGERILGLGDQGIGGMGIPIGKLSLYTACGGISPAYTLPV 193

HSP 2 Score: 24.2534 bits (51), Expect = 2.165e-41
Identity = 9/15 (60.00%), Postives = 11/15 (73.33%), Query Frame = 3 
Query:  738 GDNNEQLLEDDFYIG 782
            G NN+QLL D  Y+G
Sbjct:  198 GTNNQQLLNDPLYMG 212
[Close]
BLAST of EY710860 vs. ExPASy Swiss-Prot
Match: MAO1_SHISS (NAD-dependent malic enzyme OS=Shigella sonnei (strain Ss046) GN=sfcA PE=3 SV=2)

HSP 1 Score: 166.392 bits (420), Expect = 2.821e-41
Identity = 81/178 (45.51%), Postives = 125/178 (70.22%), Query Frame = 2 
Query:  194 SGYCLLRDPRHNKGLAFTEKERDAHYLRGLLPSSVISQQLQEKKLMISIRQYEVALQKYVAMMELEERNERLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKILEVLKNWPERSIQVIVVTDGERILGLGDLGCQGMGIPGGKLALYTALGGIRPSTCLPI 727
            +G  LL  P  NKG AF+ +ER    L GLLP  V + + Q ++  I  + ++  + K++ +  +++ NE LFY+L+ ++++E++PV+YTPTVG AC+++  I+RR +G++IS + +  + ++L+N P  +I+VIVVTDGERILGLGD G  GMGIP GKL+LYTA GGI P+  LP+
Sbjct:   16 AGPVLLEFPLLNKGSAFSMEERRNFNLLGLLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPVIYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERILGLGDQGIGGMGIPIGKLSLYTACGGISPAYTLPV 193

HSP 2 Score: 24.2534 bits (51), Expect = 2.821e-41
Identity = 9/15 (60.00%), Postives = 11/15 (73.33%), Query Frame = 3 
Query:  738 GDNNEQLLEDDFYIG 782
            G NN+QLL D  Y+G
Sbjct:  198 GTNNQQLLNDPLYMG 212
[Close]
BLAST of EY710860 vs. ExPASy Swiss-Prot
Match: MAO1_SHIFL (NAD-dependent malic enzyme OS=Shigella flexneri GN=sfcA PE=3 SV=4)

HSP 1 Score: 166.392 bits (420), Expect = 2.821e-41
Identity = 81/178 (45.51%), Postives = 125/178 (70.22%), Query Frame = 2 
Query:  194 SGYCLLRDPRHNKGLAFTEKERDAHYLRGLLPSSVISQQLQEKKLMISIRQYEVALQKYVAMMELEERNERLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKILEVLKNWPERSIQVIVVTDGERILGLGDLGCQGMGIPGGKLALYTALGGIRPSTCLPI 727
            +G  LL  P  NKG AF+ +ER    L GLLP  V + + Q ++  I  + ++  + K++ +  +++ NE LFY+L+ ++++E++PV+YTPTVG AC+++  I+RR +G++IS + +  + ++L+N P  +I+VIVVTDGERILGLGD G  GMGIP GKL+LYTA GGI P+  LP+
Sbjct:   16 AGPVLLEFPLLNKGSAFSMEERRNFNLLGLLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPVIYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERILGLGDQGIGGMGIPIGKLSLYTACGGISPAYTLPV 193

HSP 2 Score: 24.2534 bits (51), Expect = 2.821e-41
Identity = 9/15 (60.00%), Postives = 11/15 (73.33%), Query Frame = 3 
Query:  738 GDNNEQLLEDDFYIG 782
            G NN+QLL D  Y+G
Sbjct:  198 GTNNQQLLNDPLYMG 212
[Close]
BLAST of EY710860 vs. ExPASy Swiss-Prot
Match: MAO1_SHIF8 (NAD-dependent malic enzyme OS=Shigella flexneri serotype 5b (strain 8401) GN=sfcA PE=3 SV=1)

HSP 1 Score: 166.392 bits (420), Expect = 2.821e-41
Identity = 81/178 (45.51%), Postives = 125/178 (70.22%), Query Frame = 2 
Query:  194 SGYCLLRDPRHNKGLAFTEKERDAHYLRGLLPSSVISQQLQEKKLMISIRQYEVALQKYVAMMELEERNERLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKILEVLKNWPERSIQVIVVTDGERILGLGDLGCQGMGIPGGKLALYTALGGIRPSTCLPI 727
            +G  LL  P  NKG AF+ +ER    L GLLP  V + + Q ++  I  + ++  + K++ +  +++ NE LFY+L+ ++++E++PV+YTPTVG AC+++  I+RR +G++IS + +  + ++L+N P  +I+VIVVTDGERILGLGD G  GMGIP GKL+LYTA GGI P+  LP+
Sbjct:   16 AGPVLLEFPLLNKGSAFSMEERRNFNLLGLLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPVIYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERILGLGDQGIGGMGIPIGKLSLYTACGGISPAYTLPV 193

HSP 2 Score: 24.2534 bits (51), Expect = 2.821e-41
Identity = 9/15 (60.00%), Postives = 11/15 (73.33%), Query Frame = 3 
Query:  738 GDNNEQLLEDDFYIG 782
            G NN+QLL D  Y+G
Sbjct:  198 GTNNQQLLNDPLYMG 212
[Close]
BLAST of EY710860 vs. ExPASy Swiss-Prot
Match: MAO1_SHIBS (NAD-dependent malic enzyme OS=Shigella boydii serotype 4 (strain Sb227) GN=sfcA PE=3 SV=2)

HSP 1 Score: 166.392 bits (420), Expect = 2.821e-41
Identity = 81/178 (45.51%), Postives = 125/178 (70.22%), Query Frame = 2 
Query:  194 SGYCLLRDPRHNKGLAFTEKERDAHYLRGLLPSSVISQQLQEKKLMISIRQYEVALQKYVAMMELEERNERLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKILEVLKNWPERSIQVIVVTDGERILGLGDLGCQGMGIPGGKLALYTALGGIRPSTCLPI 727
            +G  LL  P  NKG AF+ +ER    L GLLP  V + + Q ++  I  + ++  + K++ +  +++ NE LFY+L+ ++++E++PV+YTPTVG AC+++  I+RR +G++IS + +  + ++L+N P  +I+VIVVTDGERILGLGD G  GMGIP GKL+LYTA GGI P+  LP+
Sbjct:   16 AGPVLLEFPLLNKGSAFSMEERRNFNLLGLLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPVIYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERILGLGDQGIGGMGIPIGKLSLYTACGGISPAYTLPV 193

HSP 2 Score: 24.2534 bits (51), Expect = 2.821e-41
Identity = 9/15 (60.00%), Postives = 11/15 (73.33%), Query Frame = 3 
Query:  738 GDNNEQLLEDDFYIG 782
            G NN+QLL D  Y+G
Sbjct:  198 GTNNQQLLNDPLYMG 212
[Close]
BLAST of EY710860 vs. ExPASy Swiss-Prot
Match: MAO1_SHIB3 (NAD-dependent malic enzyme OS=Shigella boydii serotype 18 (strain CDC 3083-94 / BS512) GN=sfcA PE=3 SV=1)

HSP 1 Score: 166.392 bits (420), Expect = 2.821e-41
Identity = 81/178 (45.51%), Postives = 125/178 (70.22%), Query Frame = 2 
Query:  194 SGYCLLRDPRHNKGLAFTEKERDAHYLRGLLPSSVISQQLQEKKLMISIRQYEVALQKYVAMMELEERNERLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKILEVLKNWPERSIQVIVVTDGERILGLGDLGCQGMGIPGGKLALYTALGGIRPSTCLPI 727
            +G  LL  P  NKG AF+ +ER    L GLLP  V + + Q ++  I  + ++  + K++ +  +++ NE LFY+L+ ++++E++PV+YTPTVG AC+++  I+RR +G++IS + +  + ++L+N P  +I+VIVVTDGERILGLGD G  GMGIP GKL+LYTA GGI P+  LP+
Sbjct:   16 AGPVLLEFPLLNKGSAFSMEERRNFNLLGLLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPVIYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERILGLGDQGIGGMGIPIGKLSLYTACGGISPAYTLPV 193

HSP 2 Score: 24.2534 bits (51), Expect = 2.821e-41
Identity = 9/15 (60.00%), Postives = 11/15 (73.33%), Query Frame = 3 
Query:  738 GDNNEQLLEDDFYIG 782
            G NN+QLL D  Y+G
Sbjct:  198 GTNNQQLLNDPLYMG 212
[Close]
BLAST of EY710860 vs. ExPASy Swiss-Prot
Match: MAO1_ESCF3 (NAD-dependent malic enzyme OS=Escherichia fergusonii (strain ATCC 35469 / DSM 13698 / CDC 0568-73) GN=sfcA PE=3 SV=1)

HSP 1 Score: 166.392 bits (420), Expect = 2.821e-41
Identity = 81/178 (45.51%), Postives = 125/178 (70.22%), Query Frame = 2 
Query:  194 SGYCLLRDPRHNKGLAFTEKERDAHYLRGLLPSSVISQQLQEKKLMISIRQYEVALQKYVAMMELEERNERLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKILEVLKNWPERSIQVIVVTDGERILGLGDLGCQGMGIPGGKLALYTALGGIRPSTCLPI 727
            +G  LL  P  NKG AF+ +ER    L GLLP  V + + Q ++  I  + ++  + K++ +  +++ NE LFY+L+ ++++E++PV+YTPTVG AC+++  I+RR +G++IS + +  + ++L+N P  +I+VIVVTDGERILGLGD G  GMGIP GKL+LYTA GGI P+  LP+
Sbjct:   16 AGPVLLEFPLLNKGSAFSMEERRNFNLLGLLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPVIYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERILGLGDQGIGGMGIPIGKLSLYTACGGISPAYTLPV 193

HSP 2 Score: 24.2534 bits (51), Expect = 2.821e-41
Identity = 9/15 (60.00%), Postives = 11/15 (73.33%), Query Frame = 3 
Query:  738 GDNNEQLLEDDFYIG 782
            G NN+QLL D  Y+G
Sbjct:  198 GTNNQQLLNDPLYMG 212
[Close]
BLAST of EY710860 vs. ExPASy Swiss-Prot
Match: MAO1_ECOUT (NAD-dependent malic enzyme OS=Escherichia coli (strain UTI89 / UPEC) GN=sfcA PE=3 SV=2)

HSP 1 Score: 166.392 bits (420), Expect = 2.821e-41
Identity = 81/178 (45.51%), Postives = 125/178 (70.22%), Query Frame = 2 
Query:  194 SGYCLLRDPRHNKGLAFTEKERDAHYLRGLLPSSVISQQLQEKKLMISIRQYEVALQKYVAMMELEERNERLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKILEVLKNWPERSIQVIVVTDGERILGLGDLGCQGMGIPGGKLALYTALGGIRPSTCLPI 727
            +G  LL  P  NKG AF+ +ER    L GLLP  V + + Q ++  I  + ++  + K++ +  +++ NE LFY+L+ ++++E++PV+YTPTVG AC+++  I+RR +G++IS + +  + ++L+N P  +I+VIVVTDGERILGLGD G  GMGIP GKL+LYTA GGI P+  LP+
Sbjct:   16 AGPVLLEFPLLNKGSAFSMEERRNFNLLGLLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPVIYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERILGLGDQGIGGMGIPIGKLSLYTACGGISPAYTLPV 193

HSP 2 Score: 24.2534 bits (51), Expect = 2.821e-41
Identity = 9/15 (60.00%), Postives = 11/15 (73.33%), Query Frame = 3 
Query:  738 GDNNEQLLEDDFYIG 782
            G NN+QLL D  Y+G
Sbjct:  198 GTNNQQLLNDPLYMG 212
The following BLAST results are available for this feature:
BLAST of EY710860 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 140
Match NameE-valueIdentityDescription
MAO1_CITK81.662e-4146.07NAD-dependent malic enzyme OS=Citrobacter koseri (... [more]
MAO1_PECCP1.869e-4147.19NAD-dependent malic enzyme OS=Pectobacterium carot... [more]
MAO1_SODGM2.165e-4145.51NAD-dependent malic enzyme OS=Sodalis glossinidius... [more]
MAO1_SHISS2.821e-4145.51NAD-dependent malic enzyme OS=Shigella sonnei (str... [more]
MAO1_SHIFL2.821e-4145.51NAD-dependent malic enzyme OS=Shigella flexneri GN... [more]
MAO1_SHIF82.821e-4145.51NAD-dependent malic enzyme OS=Shigella flexneri se... [more]
MAO1_SHIBS2.821e-4145.51NAD-dependent malic enzyme OS=Shigella boydii sero... [more]
MAO1_SHIB32.821e-4145.51NAD-dependent malic enzyme OS=Shigella boydii sero... [more]
MAO1_ESCF32.821e-4145.51NAD-dependent malic enzyme OS=Escherichia ferguson... [more]
MAO1_ECOUT2.821e-4145.51NAD-dependent malic enzyme OS=Escherichia coli (st... [more]

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Properties
Property NameValue
Genbank descriptionCS00-C3-702-001-A09-CT.F Sweet orange fruit, development stadium (3 of 6) Citrus sinensis cDNA, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>EY710860 ID=EY710860; Name=EY710860; organism=Citrus sinensis; type=EST; length=915bp
TTCTCGACACGCGCTTCCGGTAAAGAGATAACTAAAGGAGTGTTTATGGA
GAGCACCATGAAGGAGATGAGAGACGGAGCTTCTTTTATCCACTAAACTC
GTATCCACCGTCGAAGGCGGCGTTGAAGACGTCTACGGTGAGGATCGTGC
CACCGAGGACCAGCTCGTCACTCCCTGGACCATCTCTGTTGCCAGCGGGT
ACTGTTTGTTGAGGGATCCACGCCATAACAAAGGGCTTGCTTTCACTGAG
AAAGAGAGGGATGCCCACTACTTGCGCGGCCTTTTGCCTTCTTCTGTTAT
AAGTCAACAACTTCAGGAGAAGAAATTGATGATCAGCATCAGACAATATG
AAGTTGCGCTGCAAAAATATGTGGCCATGATGGAACTTGAGGAGAGAAAT
GAAAGACTGTTCTATAAGCTACTGATTGATAATGTTGAGGAACTTCTCCC
AGTCGTTTACACTCCAACTGTTGGTGAAGCTTGCCAGAAATATGGAAGTA
TCTTCAGGCGTCCTCAGGGTCTATACATAAGTCTGAAAGAGAAGGGAAAG
ATTCTTGAGGTTTTGAAGAACTGGCCTGAAAGGAGTATTCAGGTTATTGT
TGTCACAGATGGTGAGCGCATTTTGGGACTTGGGGATCTTGGCTGTCAGG
GGATGGGAATTCCTGGTGGGAAATTGGCTTTGTACACAGCACTTGGAGGC
ATTCGTCCCTCAACATGTTTGCCCATACCGGTGATGTGGGGACAACAATG
AGCAATTGTTGGAAGATGATTTTTACATTGGCCTCCACAGAGGAGGGCAC
CCGGACGGAATATGCTGACTTCTAGAGGATTCCTGAGTGGTGGCAAGCAA
AATTAGGAAAAAAGGTCCTATCCGTTTGAGGATTCCCAACCACATGTTTT
GGAGTGCTGCGAATA
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