EY710443

Overview
NameEY710443
Unique NameEY710443
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length1008
Libraries
Library NameType
Sweet orange fruit, development stadium (2 of 6)cdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of EY710443 vs. ExPASy Swiss-Prot
Match: MPG1_NEUCR (Mannose-1-phosphate guanyltransferase OS=Neurospora crassa GN=mpg-1 PE=3 SV=1)

HSP 1 Score: 85.5001 bits (210), Expect = 1.999e-37
Identity = 39/66 (59.09%), Postives = 55/66 (83.33%), Query Frame = 3 
Query:  198 HMEEASIMVTKVDEPSKYGVVVMEDS-TGKVEKFVEKPKLFVGNKINAGIYLLNPAGLDRIELRPT 392
            H +E +I+VTKV+EPSKYGVVV + +   ++++FVEKP  FVGN+INAG+Y+ NP+ +DR+ELRPT
Sbjct:  127 HGDEGTIVVTKVEEPSKYGVVVHKPNHPSRIDRFVEKPVQFVGNRINAGLYIFNPSVIDRVELRPT 192

HSP 2 Score: 77.0258 bits (188), Expect = 1.999e-37
Identity = 42/66 (63.64%), Postives = 47/66 (71.21%), Query Frame = 2 
Query:    5 FLKEFESKLGIKIICSQETEPLGTAGPLALARDKL-IDDTGEPFFVLNSDVISEYPFAEMIEFHKA 199
            +L E+E + GI I  S E+EPLGTAGPL LA D L  DDT  PFFVLNSDV  EYPF E+  FHKA
Sbjct:   63 YLAEYEKQFGINITISIESEPLGTAGPLKLAEDVLRKDDT--PFFVLNSDVTCEYPFKELAAFHKA 126

HSP 3 Score: 31.187 bits (69), Expect = 1.999e-37
Identity = 11/17 (64.71%), Postives = 13/17 (76.47%), Query Frame = 2 
Query:  458 LPSFWRDIGQPRDYFTG 508
            L  FW DIGQP+D+ TG
Sbjct:  213 LEGFWMDIGQPKDFLTG 229

HSP 4 Score: 24.6386 bits (52), Expect = 1.999e-37
Identity = 13/32 (40.62%), Postives = 17/32 (53.12%), Query Frame = 1 
Query:  388 PPSIEKEVFPKIALEGKSSFRDGSSKFLEGHW 483
            P SIE+E FP +  +G+    D     LEG W
Sbjct:  191 PTSIEQETFPAMVRDGQLHSFD-----LEGFW 217
[Close]
BLAST of EY710443 vs. ExPASy Swiss-Prot
Match: MPG1_YEAST (Mannose-1-phosphate guanyltransferase OS=Saccharomyces cerevisiae GN=MPG1 PE=1 SV=2)

HSP 1 Score: 81.2629 bits (199), Expect = 4.473e-37
Identity = 38/66 (57.58%), Postives = 53/66 (80.30%), Query Frame = 3 
Query:  198 HMEEASIMVTKVDEPSKYGVVVMEDSTGK-VEKFVEKPKLFVGNKINAGIYLLNPAGLDRIELRPT 392
            H  + +I+ TKVDEPSKYGV+V + +T   +++FVEKPK FVGN+INAG+Y+LNP  +D IE++PT
Sbjct:  127 HGGKGTIVATKVDEPSKYGVIVHDIATPNLIDRFVEKPKEFVGNRINAGLYILNPEVIDLIEMKPT 192

HSP 2 Score: 80.8777 bits (198), Expect = 4.473e-37
Identity = 41/64 (64.06%), Postives = 47/64 (73.44%), Query Frame = 2 
Query:    8 LKEFESKLGIKIICSQETEPLGTAGPLALARDKLIDDTGEPFFVLNSDVISEYPFAEMIEFHKA 199
            LK++E + G+ I  S ETEPLGTAGPL LA D L  D   PFFVLNSDVI EYPF E+ +FHKA
Sbjct:   64 LKKYEKEYGVNITFSVETEPLGTAGPLKLAEDVLKKD-NSPFFVLNSDVICEYPFKELADFHKA 126

HSP 3 Score: 35.039 bits (79), Expect = 4.473e-37
Identity = 21/58 (36.21%), Postives = 31/58 (53.45%), Query Frame = 2 
Query:  398 LRKRSFQKLL*RGKAPFAMVLPSFWRDIGQPRDYFTGLRTLSGTP*GKKPPFKWATGA 571
            + K +F  L+   K  ++  L  FW D+GQP+D+ +G   L      K+ P K ATGA
Sbjct:  194 IEKETFPILV-EEKQLYSFDLEGFWMDVGQPKDFLSG-TVLYLNSLAKRQPKKLATGA 249
[Close]
BLAST of EY710443 vs. ExPASy Swiss-Prot
Match: GMPPB_DROME (Mannose-1-phosphate guanyltransferase beta OS=Drosophila melanogaster GN=CG1129 PE=2 SV=1)

HSP 1 Score: 84.7297 bits (208), Expect = 9.813e-37
Identity = 40/65 (61.54%), Postives = 53/65 (81.54%), Query Frame = 3 
Query:  198 HMEEASIMVTKVDEPSKYGVVVMEDSTGKVEKFVEKPKLFVGNKINAGIYLLNPAGLDRIELRPT 392
            H +E +I+VTKV+EPSKYGVV+  D  G ++ F+EKP+ FV NKINAGIY+ NP+ LDRIE++PT
Sbjct:  137 HGKEGTIVVTKVEEPSKYGVVLY-DENGCIKNFIEKPQEFVSNKINAGIYIFNPSVLDRIEVKPT 200

HSP 2 Score: 75.8702 bits (185), Expect = 9.813e-37
Identity = 36/62 (58.06%), Postives = 49/62 (79.03%), Query Frame = 2 
Query:    8 LKEFESKLGIKIICSQETEPLGTAGPLALARDKLIDDTGEPFFVLNSDVISEYPFAEMIEFH 193
            LK    KLG+++I S ETEPLGTAGPLALA+  ++  + EPFFVLNSDVI ++PF ++++FH
Sbjct:   74 LKVEAKKLGVELIFSHETEPLGTAGPLALAK-TILAASSEPFFVLNSDVICDFPFKQLVQFH 134

HSP 3 Score: 35.4242 bits (80), Expect = 9.813e-37
Identity = 13/22 (59.09%), Postives = 17/22 (77.27%), Query Frame = 2 
Query:  446 FAMVLPSFWRDIGQPRDYFTGL 511
            +AM L  FW DIGQP+D+ TG+
Sbjct:  217 YAMDLTGFWMDIGQPKDFLTGM 238
[Close]
BLAST of EY710443 vs. ExPASy Swiss-Prot
Match: MPG1_ASPOR (Mannose-1-phosphate guanyltransferase OS=Aspergillus oryzae GN=mpg1 PE=3 SV=1)

HSP 1 Score: 89.3521 bits (220), Expect = 3.639e-36
Identity = 43/67 (64.18%), Postives = 57/67 (85.07%), Query Frame = 3 
Query:  195 KHMEEASIMVTKVDEPSKYGVVVMEDS-TGKVEKFVEKPKLFVGNKINAGIYLLNPAGLDRIELRPT 392
            KH +E +I+VTKVDEPSKYGVVV + +   ++++FVEKP  FVGN+INAGIY++NP+ L+RIELRPT
Sbjct:  126 KHGDEGTIVVTKVDEPSKYGVVVHKPNHPSRIDRFVEKPVEFVGNRINAGIYIMNPSVLNRIELRPT 192

HSP 2 Score: 75.485 bits (184), Expect = 3.639e-36
Identity = 37/63 (58.73%), Postives = 47/63 (74.60%), Query Frame = 2 
Query:    8 LKEFESKLGIKIICSQETEPLGTAGPLALARDKLIDDTGEPFFVLNSDVISEYPFAEMIEFHK 196
            LK++E +  +KI  S E+EPLGTAGPL LA +K++     PFFVLNSDVI +YPF E+ EFHK
Sbjct:   64 LKKYEEQYNVKIEFSVESEPLGTAGPLKLA-EKILGKDDSPFFVLNSDVICDYPFKELAEFHK 125

HSP 3 Score: 29.261 bits (64), Expect = 3.639e-36
Identity = 9/17 (52.94%), Postives = 13/17 (76.47%), Query Frame = 2 
Query:  458 LPSFWRDIGQPRDYFTG 508
            L  FW D+GQP+D+ +G
Sbjct:  213 LEGFWMDVGQPKDFLSG 229
[Close]
BLAST of EY710443 vs. ExPASy Swiss-Prot
Match: MPG12_CANGA (Mannose-1-phosphate guanyltransferase 2 OS=Candida glabrata GN=MPG1 PE=3 SV=1)

HSP 1 Score: 80.4925 bits (197), Expect = 4.731e-36
Identity = 38/66 (57.58%), Postives = 52/66 (78.79%), Query Frame = 3 
Query:  198 HMEEASIMVTKVDEPSKYGVVVMEDSTGK-VEKFVEKPKLFVGNKINAGIYLLNPAGLDRIELRPT 392
            H  + +I+ TKVDEPSKYGV+V +  T   +++FVEKPK FVGN+INAG+Y+LNP  +D IE++PT
Sbjct:  127 HGGKGTIVATKVDEPSKYGVIVHDLGTPNLIDRFVEKPKEFVGNRINAGLYILNPEVIDLIEMKPT 192

HSP 2 Score: 80.1073 bits (196), Expect = 4.731e-36
Identity = 39/64 (60.94%), Postives = 48/64 (75.00%), Query Frame = 2 
Query:    8 LKEFESKLGIKIICSQETEPLGTAGPLALARDKLIDDTGEPFFVLNSDVISEYPFAEMIEFHKA 199
            LK++E + G+ I  S ETEPLGTAGPL LA +K++     PFFVLNSDVI EYPF E+ EFHK+
Sbjct:   64 LKKYEKEYGVNITFSVETEPLGTAGPLKLA-EKILKKDNSPFFVLNSDVICEYPFKELAEFHKS 126

HSP 3 Score: 33.113 bits (74), Expect = 4.731e-36
Identity = 20/62 (32.26%), Postives = 34/62 (54.84%), Query Frame = 2 
Query:  398 LRKRSFQKLL*RGKAPFAMVLPSFWRDIGQPRDYFTG----LRTLSGTP*GKKPPFKWATGA 571
            +   +F KL+   K+ +   L  FW D+GQP+D+  G    L++LS     ++ P K +TG+
Sbjct:  194 IETETFPKLV-NEKSLYTFDLEGFWMDVGQPKDFLAGTGLYLQSLS-----RRHPEKLSTGS 249
[Close]
BLAST of EY710443 vs. ExPASy Swiss-Prot
Match: MPG1_ASPFU (Mannose-1-phosphate guanyltransferase OS=Aspergillus fumigatus GN=mpg1 PE=2 SV=1)

HSP 1 Score: 89.3521 bits (220), Expect = 7.987e-36
Identity = 44/67 (65.67%), Postives = 56/67 (83.58%), Query Frame = 3 
Query:  195 KHMEEASIMVTKVDEPSKYGVVVMEDS-TGKVEKFVEKPKLFVGNKINAGIYLLNPAGLDRIELRPT 392
            KH +E +I+VTKVDEPSKYGVVV + +   ++++FVEKP  FVGN+INAGIY+LNP+ L RIELRPT
Sbjct:  126 KHGDEGTIVVTKVDEPSKYGVVVHKPNHPSRIDRFVEKPVEFVGNRINAGIYILNPSVLKRIELRPT 192

HSP 2 Score: 72.7886 bits (177), Expect = 7.987e-36
Identity = 34/63 (53.97%), Postives = 47/63 (74.60%), Query Frame = 2 
Query:    8 LKEFESKLGIKIICSQETEPLGTAGPLALARDKLIDDTGEPFFVLNSDVISEYPFAEMIEFHK 196
            LK++E +  ++I  S E+EPLGTAGPL LA +K++     PFFVLNSD+I +YPF ++ EFHK
Sbjct:   64 LKKYEEQYNVRIEFSVESEPLGTAGPLKLA-EKILGKDDSPFFVLNSDIICDYPFKQLAEFHK 125

HSP 3 Score: 30.8018 bits (68), Expect = 7.987e-36
Identity = 10/17 (58.82%), Postives = 13/17 (76.47%), Query Frame = 2 
Query:  458 LPSFWRDIGQPRDYFTG 508
            L  FW D+GQP+D+ TG
Sbjct:  213 LEGFWMDVGQPKDFLTG 229
[Close]
BLAST of EY710443 vs. ExPASy Swiss-Prot
Match: MPG1_EMENI (Mannose-1-phosphate guanyltransferase OS=Emericella nidulans GN=mpg1 PE=3 SV=1)

HSP 1 Score: 87.0409 bits (214), Expect = 1.039e-35
Identity = 42/67 (62.69%), Postives = 56/67 (83.58%), Query Frame = 3 
Query:  195 KHMEEASIMVTKVDEPSKYGVVVMEDS-TGKVEKFVEKPKLFVGNKINAGIYLLNPAGLDRIELRPT 392
            +H +E +I+VTKVDEPSKYGVVV + +   ++++FVEKP  FVGN+INAG+Y+LNP+ L RIELRPT
Sbjct:  113 RHGDEGTIVVTKVDEPSKYGVVVHKPNHPSRIDRFVEKPVEFVGNRINAGMYILNPSVLKRIELRPT 179

HSP 2 Score: 74.7146 bits (182), Expect = 1.039e-35
Identity = 38/63 (60.32%), Postives = 45/63 (71.43%), Query Frame = 2 
Query:    8 LKEFESKLGIKIICSQETEPLGTAGPLALARDKLIDDTGEPFFVLNSDVISEYPFAEMIEFHK 196
            LK++E +  +KI  S ETEPLGTAGPL LA   L  D   PFFVLNSDVI +YPF ++ EFHK
Sbjct:   51 LKKYEEQYNVKIEFSVETEPLGTAGPLKLAESILAKDDS-PFFVLNSDVICDYPFQQLAEFHK 112

HSP 3 Score: 30.8018 bits (68), Expect = 1.039e-35
Identity = 10/17 (58.82%), Postives = 13/17 (76.47%), Query Frame = 2 
Query:  458 LPSFWRDIGQPRDYFTG 508
            L  FW D+GQP+D+ TG
Sbjct:  200 LEGFWMDVGQPKDFLTG 216
[Close]
BLAST of EY710443 vs. ExPASy Swiss-Prot
Match: MPG1_YARLI (Mannose-1-phosphate guanyltransferase OS=Yarrowia lipolytica GN=MPG1 PE=3 SV=1)

HSP 1 Score: 90.1225 bits (222), Expect = 1.745e-35
Identity = 63/163 (38.65%), Postives = 92/163 (56.44%), Query Frame = 3 
Query:  195 KHMEEASIMVTKVDEPSKYGVVVMEDSTGKVEKFVEKPKLFVGNKINAGIYLLNPAGLDRIELRPTLH*ERGLSKNCFRGEKLLSR---WFFQVSGGTLD--SQGITLQA*ELYLELPEEKNLHSSGPLGHKLEGNVLGHENGSILGEGCFEMEPKVAVRPRV 668
            K+  EA+I+ TKV+EPSKYGV+V +  T K+++FVEKP  FVGN+INAGIY+LNP+ +D I+LRPT      + K  F   +L +R   + F + G  +D       L    LYL    +KN  +  P     E  V G   G++L +   ++ P+  + P V
Sbjct:  126 KNNAEATIVATKVEEPSKYGVIVHKQGTSKIDRFVEKPVEFVGNRINAGIYILNPSVVDLIDLRPT-----SIEKETF--PQLAARESLYSFDLEGYWMDVGQPKDFLSGTCLYLSSLSKKNPEALVPTS---EPYVTG---GNVLVDPTAKISPQAKIGPNV 275

HSP 2 Score: 81.2629 bits (199), Expect = 1.745e-35
Identity = 43/64 (67.19%), Postives = 49/64 (76.56%), Query Frame = 2 
Query:    8 LKEFESKLGIKIICSQETEPLGTAGPLALARDKLI-DDTGEPFFVLNSDVISEYPFAEMIEFHK 196
            LK++E K G+ I  S ETEPLGTAGPL LA + L  DDT  PFFVLNSDVI +YPFAE+ EFHK
Sbjct:   64 LKKYEEKYGVNITFSVETEPLGTAGPLKLAEEILCKDDT--PFFVLNSDVICDYPFAELAEFHK 125
[Close]
BLAST of EY710443 vs. ExPASy Swiss-Prot
Match: MPG11_CANGA (Mannose-1-phosphate guanyltransferase 1 OS=Candida glabrata GN=MPG1 PE=1 SV=1)

HSP 1 Score: 81.2629 bits (199), Expect = 4.998e-35
Identity = 38/66 (57.58%), Postives = 53/66 (80.30%), Query Frame = 3 
Query:  198 HMEEASIMVTKVDEPSKYGVVVMEDSTGK-VEKFVEKPKLFVGNKINAGIYLLNPAGLDRIELRPT 392
            H  + +I+ TKVDEPSKYGV+V + +T   +++FVEKPK FVGN+INAG+Y+LNP  +D IE++PT
Sbjct:  127 HGGKGTIVATKVDEPSKYGVIVHDIATPNLIDRFVEKPKEFVGNRINAGLYILNPEVIDLIEMKPT 192

HSP 2 Score: 78.1814 bits (191), Expect = 4.998e-35
Identity = 38/64 (59.38%), Postives = 48/64 (75.00%), Query Frame = 2 
Query:    8 LKEFESKLGIKIICSQETEPLGTAGPLALARDKLIDDTGEPFFVLNSDVISEYPFAEMIEFHKA 199
            L+++E + G+ I  S ETEPLGTAGPL LA +K++     PFFVLNSDVI EYPF E+ +FHKA
Sbjct:   64 LQKYEKEYGVSITFSVETEPLGTAGPLKLA-EKVLKKDNSPFFVLNSDVICEYPFKELADFHKA 126

HSP 3 Score: 30.8018 bits (68), Expect = 4.998e-35
Identity = 10/24 (41.67%), Postives = 17/24 (70.83%), Query Frame = 2 
Query:  437 KAPFAMVLPSFWRDIGQPRDYFTG 508
            K+ ++  L  FW D+GQP+D+ +G
Sbjct:  206 KSLYSFDLEGFWMDVGQPKDFLSG 229
[Close]
BLAST of EY710443 vs. ExPASy Swiss-Prot
Match: MPG1_TRIRE (Mannose-1-phosphate guanyltransferase OS=Trichoderma reesei GN=mpg1 PE=1 SV=1)

HSP 1 Score: 83.1889 bits (204), Expect = 1.851e-34
Identity = 40/66 (60.61%), Postives = 54/66 (81.82%), Query Frame = 3 
Query:  198 HMEEASIMVTKVDEPSKYGVVVMEDS-TGKVEKFVEKPKLFVGNKINAGIYLLNPAGLDRIELRPT 392
            H +E +I+VTKV+EPSKYGVVV + +   ++++FVEKP  FVGN+INAG+Y+ NP+ L RIELRPT
Sbjct:  127 HGDEGTIVVTKVEEPSKYGVVVHKPNHPSRIDRFVEKPVEFVGNRINAGMYIFNPSVLKRIELRPT 192

HSP 2 Score: 75.8702 bits (185), Expect = 1.851e-34
Identity = 38/65 (58.46%), Postives = 47/65 (72.31%), Query Frame = 2 
Query:    5 FLKEFESKLGIKIICSQETEPLGTAGPLALARDKLIDDTGEPFFVLNSDVISEYPFAEMIEFHKA 199
            FL E+E K  I I  S E+EPL TAGPL LA ++++     PFFVLNSDVI +YPF E++EFHKA
Sbjct:   63 FLAEYEEKYNINIEFSVESEPLDTAGPLKLA-ERILGKDDSPFFVLNSDVICDYPFKELLEFHKA 126

HSP 3 Score: 29.261 bits (64), Expect = 1.851e-34
Identity = 9/17 (52.94%), Postives = 13/17 (76.47%), Query Frame = 2 
Query:  458 LPSFWRDIGQPRDYFTG 508
            L  FW D+GQP+D+ +G
Sbjct:  213 LEGFWMDVGQPKDFLSG 229
The following BLAST results are available for this feature:
BLAST of EY710443 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 39
Match NameE-valueIdentityDescription
MPG1_NEUCR1.999e-3759.09Mannose-1-phosphate guanyltransferase OS=Neurospor... [more]
MPG1_YEAST4.473e-3757.58Mannose-1-phosphate guanyltransferase OS=Saccharom... [more]
GMPPB_DROME9.813e-3761.54Mannose-1-phosphate guanyltransferase beta OS=Dros... [more]
MPG1_ASPOR3.639e-3664.18Mannose-1-phosphate guanyltransferase OS=Aspergill... [more]
MPG12_CANGA4.731e-3657.58Mannose-1-phosphate guanyltransferase 2 OS=Candida... [more]
MPG1_ASPFU7.987e-3665.67Mannose-1-phosphate guanyltransferase OS=Aspergill... [more]
MPG1_EMENI1.039e-3562.69Mannose-1-phosphate guanyltransferase OS=Emericell... [more]
MPG1_YARLI1.745e-3538.65Mannose-1-phosphate guanyltransferase OS=Yarrowia ... [more]
MPG11_CANGA4.998e-3557.58Mannose-1-phosphate guanyltransferase 1 OS=Candida... [more]
MPG1_TRIRE1.851e-3460.61Mannose-1-phosphate guanyltransferase OS=Trichoder... [more]

Pages

back to top
Properties
Property NameValue
Genbank descriptionCS00-C3-701-115-C07-CT.F Sweet orange fruit, development stadium (2 of 6) Citrus sinensis cDNA, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>EY710443 ID=EY710443; Name=EY710443; organism=Citrus sinensis; type=EST; length=1008bp
GAACTTTTTGAAGGAATTCGAATCAAAGCTTGGGATCAAGATCATCTGCT
CACAAGAGACTGAGCCACTGGGCACTGCTGGTCCTCTGGCTCTGGCTAGA
GACAAATTGATTGACGATACTGGTGAGCCATTTTTCGTCCTTAACAGTGA
TGTTATTAGTGAGTACCCATTTGCAGAAATGATTGAATTCCACAAAGCAC
ATGGAGGAGGCCTCCATCATGGTAACCAAGGTGGATGAGCCATCAAAATA
TGGTGTGGTGGTTATGGAAGACTCTACTGGAAAAGTTGAGAAATTCGTAG
AGAAACCTAAATTATTTGTTGGTAACAAAATTAATGCTGGAATTTATCTT
TTGAACCCTGCGGGTCTTGATCGCATTGAGCTGAGGCCCACCCTCCATTG
AGAAAGAGGTCTTTCCAAAAATTGCTTTAGAGGGGAAAAGCTCCTTTCGC
GATGGTTCTTCCAAGTTTCTGGAGGGACATTGGACAGCCAAGGGATTACT
TTACAGGCTTGAGAACTCTATCTGGAACTCCCTGAGGAAAAAAACCTCCA
TTCAAGTGGGCCACTGGGGCATAAATTGGAAGGCAATGTCTTGGGGCATG
AAAATGGCTCCATTTTGGGGGAGGGATGTTTTGAAATGGAACCAAAGGTT
GCCGTTAGGCCCCGGGTTGGATGTTGTTAAAATCAGGAAATTAAAACTCA
CATCATTGCACATAGGAATCCTGGGGAAAGGGACAACCAAAAAATATAGC
CTGCACTTATCTAATAGAATACAATTAGGGAGGGCATTTCCTTGGATGGG
TGACATAGGGGGCTCGGAGAATAAGAAACAAATTGCCCTAACTTCGTGGG
GAAAGAAACCTTTCATTGATCGTCGAAAATAAGAAATTTTTACAAGAACA
CGGGGGGGGGGGGTGTGGATTTGGTGACCCGGCGCCAGCAGGCGAACATA
AAAAACTACCCCAAACTATACTCATTATATGAAGGTCTCCCCCTAAGAGG
ACCATTCG
back to top