EY685266

Overview
NameEY685266
Unique NameEY685266
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length900
Libraries
Library NameType
Sweet orange bark, greenhouse plantcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of EY685266 vs. ExPASy Swiss-Prot
Match: TYSY_RHILO (Thymidylate synthase OS=Rhizobium loti GN=thyA PE=3 SV=1)

HSP 1 Score: 175.252 bits (443), Expect = 4.331e-43
Identity = 80/142 (56.34%), Postives = 103/142 (72.54%), Query Frame = 3 
Query:   18 PNDRRIVLSAWNPSDLKLMALPPCHMFAQFYMANGELSCQMYQRSADMGLGVPFNIASYALLTCMIAHVCDLAPGDFIHVIGDAHVYRNHVRPLEEQLQKLPKPFPILKINPEKKDIDSFVAEDFKLIGYDPHQKIEMKMAV 443
            P  RR+++SAWNP++++ MALPPCH   QFY++ G LSCQ+YQRSAD+ LGVPFNIASYALLT M+A V  L PGDF+H +GDAH+Y NH     EQLQ+ P+  P + INPE KD+ +F  EDF+L  Y     I+  +AV
Sbjct:  123 PQSRRLIVSAWNPAEVEAMALPPCHCLFQFYVSEGRLSCQLYQRSADIFLGVPFNIASYALLTLMVAQVTGLKPGDFVHTLGDAHLYSNHFEQAREQLQRTPRALPTMWINPEVKDLFAFRFEDFRLENYVADASIQAPIAV 264
[Close]
BLAST of EY685266 vs. ExPASy Swiss-Prot
Match: TYSY_BRUSU (Thymidylate synthase OS=Brucella suis GN=thyA PE=3 SV=1)

HSP 1 Score: 175.252 bits (443), Expect = 4.331e-43
Identity = 82/142 (57.75%), Postives = 103/142 (72.54%), Query Frame = 3 
Query:   18 PNDRRIVLSAWNPSDLKLMALPPCHMFAQFYMANGELSCQMYQRSADMGLGVPFNIASYALLTCMIAHVCDLAPGDFIHVIGDAHVYRNHVRPLEEQLQKLPKPFPILKINPEKKDIDSFVAEDFKLIGYDPHQKIEMKMAV 443
            P  RR+++SAWNP+ +  MALPPCH   QFY+ANG LSCQ+YQRSAD+ LGVPFNIASYALLT MIA V  L PG+FIH +GDAH+Y NH      QL + PK  P++ INP+ KD+ +F  EDF+L GY+    I+  +AV
Sbjct:  123 PQSRRLIVSAWNPALVDEMALPPCHCLFQFYVANGRLSCQLYQRSADIFLGVPFNIASYALLTMMIAQVTGLKPGEFIHTLGDAHIYSNHFEQARLQLTRTPKKLPVMHINPDVKDLFAFRFEDFRLDGYEADPTIKAPIAV 264
[Close]
BLAST of EY685266 vs. ExPASy Swiss-Prot
Match: TYSY_BRUSI (Thymidylate synthase OS=Brucella suis (strain ATCC 23445 / NCTC 10510) GN=thyA PE=3 SV=1)

HSP 1 Score: 175.252 bits (443), Expect = 4.331e-43
Identity = 82/142 (57.75%), Postives = 103/142 (72.54%), Query Frame = 3 
Query:   18 PNDRRIVLSAWNPSDLKLMALPPCHMFAQFYMANGELSCQMYQRSADMGLGVPFNIASYALLTCMIAHVCDLAPGDFIHVIGDAHVYRNHVRPLEEQLQKLPKPFPILKINPEKKDIDSFVAEDFKLIGYDPHQKIEMKMAV 443
            P  RR+++SAWNP+ +  MALPPCH   QFY+ANG LSCQ+YQRSAD+ LGVPFNIASYALLT MIA V  L PG+FIH +GDAH+Y NH      QL + PK  P++ INP+ KD+ +F  EDF+L GY+    I+  +AV
Sbjct:  123 PQSRRLIVSAWNPALVDEMALPPCHCLFQFYVANGRLSCQLYQRSADIFLGVPFNIASYALLTMMIAQVTGLKPGEFIHTLGDAHIYSNHFEQARLQLTRTPKKLPVMHINPDVKDLFAFRFEDFRLDGYEADPTIKAPIAV 264
[Close]
BLAST of EY685266 vs. ExPASy Swiss-Prot
Match: TYSY_BRUO2 (Thymidylate synthase OS=Brucella ovis (strain ATCC 25840 / 63/290 / NCTC 10512) GN=thyA PE=3 SV=1)

HSP 1 Score: 175.252 bits (443), Expect = 4.331e-43
Identity = 82/142 (57.75%), Postives = 103/142 (72.54%), Query Frame = 3 
Query:   18 PNDRRIVLSAWNPSDLKLMALPPCHMFAQFYMANGELSCQMYQRSADMGLGVPFNIASYALLTCMIAHVCDLAPGDFIHVIGDAHVYRNHVRPLEEQLQKLPKPFPILKINPEKKDIDSFVAEDFKLIGYDPHQKIEMKMAV 443
            P  RR+++SAWNP+ +  MALPPCH   QFY+ANG LSCQ+YQRSAD+ LGVPFNIASYALLT MIA V  L PG+FIH +GDAH+Y NH      QL + PK  P++ INP+ KD+ +F  EDF+L GY+    I+  +AV
Sbjct:  123 PQSRRLIVSAWNPALVDEMALPPCHCLFQFYVANGRLSCQLYQRSADIFLGVPFNIASYALLTMMIAQVTGLKPGEFIHTLGDAHIYSNHFEQARLQLTRTPKKLPVMHINPDVKDLFAFRFEDFRLDGYEADPTIKAPIAV 264
[Close]
BLAST of EY685266 vs. ExPASy Swiss-Prot
Match: TYSY_BRUME (Thymidylate synthase OS=Brucella melitensis GN=thyA PE=1 SV=1)

HSP 1 Score: 175.252 bits (443), Expect = 4.331e-43
Identity = 82/142 (57.75%), Postives = 103/142 (72.54%), Query Frame = 3 
Query:   18 PNDRRIVLSAWNPSDLKLMALPPCHMFAQFYMANGELSCQMYQRSADMGLGVPFNIASYALLTCMIAHVCDLAPGDFIHVIGDAHVYRNHVRPLEEQLQKLPKPFPILKINPEKKDIDSFVAEDFKLIGYDPHQKIEMKMAV 443
            P  RR+++SAWNP+ +  MALPPCH   QFY+ANG LSCQ+YQRSAD+ LGVPFNIASYALLT MIA V  L PG+FIH +GDAH+Y NH      QL + PK  P++ INP+ KD+ +F  EDF+L GY+    I+  +AV
Sbjct:  123 PQSRRLIVSAWNPALVDEMALPPCHCLFQFYVANGRLSCQLYQRSADIFLGVPFNIASYALLTMMIAQVTGLKPGEFIHTLGDAHIYSNHFEQARLQLTRTPKKLPVMHINPDVKDLFAFRFEDFRLDGYEADPTIKAPIAV 264
[Close]
BLAST of EY685266 vs. ExPASy Swiss-Prot
Match: TYSY_BRUMB (Thymidylate synthase OS=Brucella melitensis biotype 2 (strain ATCC 23457) GN=thyA PE=3 SV=1)

HSP 1 Score: 175.252 bits (443), Expect = 4.331e-43
Identity = 82/142 (57.75%), Postives = 103/142 (72.54%), Query Frame = 3 
Query:   18 PNDRRIVLSAWNPSDLKLMALPPCHMFAQFYMANGELSCQMYQRSADMGLGVPFNIASYALLTCMIAHVCDLAPGDFIHVIGDAHVYRNHVRPLEEQLQKLPKPFPILKINPEKKDIDSFVAEDFKLIGYDPHQKIEMKMAV 443
            P  RR+++SAWNP+ +  MALPPCH   QFY+ANG LSCQ+YQRSAD+ LGVPFNIASYALLT MIA V  L PG+FIH +GDAH+Y NH      QL + PK  P++ INP+ KD+ +F  EDF+L GY+    I+  +AV
Sbjct:  123 PQSRRLIVSAWNPALVDEMALPPCHCLFQFYVANGRLSCQLYQRSADIFLGVPFNIASYALLTMMIAQVTGLKPGEFIHTLGDAHIYSNHFEQARLQLTRTPKKLPVMHINPDVKDLFAFRFEDFRLDGYEADPTIKAPIAV 264
[Close]
BLAST of EY685266 vs. ExPASy Swiss-Prot
Match: TYSY_BRUAB (Thymidylate synthase OS=Brucella abortus GN=thyA PE=3 SV=1)

HSP 1 Score: 175.252 bits (443), Expect = 4.331e-43
Identity = 82/142 (57.75%), Postives = 103/142 (72.54%), Query Frame = 3 
Query:   18 PNDRRIVLSAWNPSDLKLMALPPCHMFAQFYMANGELSCQMYQRSADMGLGVPFNIASYALLTCMIAHVCDLAPGDFIHVIGDAHVYRNHVRPLEEQLQKLPKPFPILKINPEKKDIDSFVAEDFKLIGYDPHQKIEMKMAV 443
            P  RR+++SAWNP+ +  MALPPCH   QFY+ANG LSCQ+YQRSAD+ LGVPFNIASYALLT MIA V  L PG+FIH +GDAH+Y NH      QL + PK  P++ INP+ KD+ +F  EDF+L GY+    I+  +AV
Sbjct:  123 PQSRRLIVSAWNPALVDEMALPPCHCLFQFYVANGRLSCQLYQRSADIFLGVPFNIASYALLTMMIAQVTGLKPGEFIHTLGDAHIYSNHFEQARLQLTRTPKKLPVMHINPDVKDLFAFRFEDFRLDGYEADPTIKAPIAV 264
[Close]
BLAST of EY685266 vs. ExPASy Swiss-Prot
Match: TYSY_BRUA2 (Thymidylate synthase OS=Brucella abortus (strain 2308) GN=thyA PE=3 SV=1)

HSP 1 Score: 175.252 bits (443), Expect = 4.331e-43
Identity = 82/142 (57.75%), Postives = 103/142 (72.54%), Query Frame = 3 
Query:   18 PNDRRIVLSAWNPSDLKLMALPPCHMFAQFYMANGELSCQMYQRSADMGLGVPFNIASYALLTCMIAHVCDLAPGDFIHVIGDAHVYRNHVRPLEEQLQKLPKPFPILKINPEKKDIDSFVAEDFKLIGYDPHQKIEMKMAV 443
            P  RR+++SAWNP+ +  MALPPCH   QFY+ANG LSCQ+YQRSAD+ LGVPFNIASYALLT MIA V  L PG+FIH +GDAH+Y NH      QL + PK  P++ INP+ KD+ +F  EDF+L GY+    I+  +AV
Sbjct:  123 PQSRRLIVSAWNPALVDEMALPPCHCLFQFYVANGRLSCQLYQRSADIFLGVPFNIASYALLTMMIAQVTGLKPGEFIHTLGDAHIYSNHFEQARLQLTRTPKKLPVMHINPDVKDLFAFRFEDFRLDGYEADPTIKAPIAV 264
[Close]
BLAST of EY685266 vs. ExPASy Swiss-Prot
Match: TYSY_XYLFA (Thymidylate synthase OS=Xylella fastidiosa GN=thyA PE=3 SV=1)

HSP 1 Score: 174.866 bits (442), Expect = 5.657e-43
Identity = 81/142 (57.04%), Postives = 105/142 (73.94%), Query Frame = 3 
Query:   18 PNDRRIVLSAWNPSDLKLMALPPCHMFAQFYMANGELSCQMYQRSADMGLGVPFNIASYALLTCMIAHVCDLAPGDFIHVIGDAHVYRNHVRPLEEQLQKLPKPFPILKINPEKKDIDSFVAEDFKLIGYDPHQKIEMKMAV 443
            P+ RR+V+SAWN +DL  MAL PCH   QFY+ANG+LSCQ+YQRSAD+ LGVPFNIASYALLT M+A V  LA GDF+H +GDAH+Y NHV     QL+++P+P P+L +NP   D+  F  +D  + GYDP+  I+  +AV
Sbjct:  123 PDSRRLVISAWNVADLPQMALVPCHALFQFYVANGKLSCQLYQRSADIFLGVPFNIASYALLTHMLAQVTGLAVGDFVHTLGDAHLYANHVEQASVQLERVPRPQPVLMLNPAVTDLFDFTYDDIVIEGYDPYPAIKAPVAV 264
[Close]
BLAST of EY685266 vs. ExPASy Swiss-Prot
Match: TYSY_LACC3 (Thymidylate synthase OS=Lactobacillus casei (strain ATCC 334) GN=thyA PE=3 SV=1)

HSP 1 Score: 174.866 bits (442), Expect = 5.657e-43
Identity = 80/143 (55.94%), Postives = 105/143 (73.43%), Query Frame = 3 
Query:   15 YPNDRRIVLSAWNPSDLKLMALPPCHMFAQFYMANGELSCQMYQRSADMGLGVPFNIASYALLTCMIAHVCDLAPGDFIHVIGDAHVYRNHVRPLEEQLQKLPKPFPILKINPEKKDIDSFVAEDFKLIGYDPHQKIEMKMAV 443
            +P  RR+++SAWNP D+  MALPPCH   QFY+ +G+LS Q+YQRSAD+ LGVPFNIASYALLT ++AH C L  GDFIH  GDAH+Y NH+  ++EQL + P+  P L +NP+K DI  F  +D KL+ YDP+  I+  +AV
Sbjct:  174 HPYSRRLIVSAWNPEDVPTMALPPCHTLFQFYVNDGKLSLQLYQRSADIFLGVPFNIASYALLTHLVAHECGLQVGDFIHTFGDAHLYVNHLDQIKEQLTRTPRQAPTLVLNPDKHDIFDFDMQDIKLLNYDPYPAIKAPVAV 316
The following BLAST results are available for this feature:
BLAST of EY685266 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 405
Match NameE-valueIdentityDescription
TYSY_RHILO4.331e-4356.34Thymidylate synthase OS=Rhizobium loti GN=thyA PE=... [more]
TYSY_BRUSU4.331e-4357.75Thymidylate synthase OS=Brucella suis GN=thyA PE=3... [more]
TYSY_BRUSI4.331e-4357.75Thymidylate synthase OS=Brucella suis (strain ATCC... [more]
TYSY_BRUO24.331e-4357.75Thymidylate synthase OS=Brucella ovis (strain ATCC... [more]
TYSY_BRUME4.331e-4357.75Thymidylate synthase OS=Brucella melitensis GN=thy... [more]
TYSY_BRUMB4.331e-4357.75Thymidylate synthase OS=Brucella melitensis biotyp... [more]
TYSY_BRUAB4.331e-4357.75Thymidylate synthase OS=Brucella abortus GN=thyA P... [more]
TYSY_BRUA24.331e-4357.75Thymidylate synthase OS=Brucella abortus (strain 2... [more]
TYSY_XYLFA5.657e-4357.04Thymidylate synthase OS=Xylella fastidiosa GN=thyA... [more]
TYSY_LACC35.657e-4355.94Thymidylate synthase OS=Lactobacillus casei (strai... [more]

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Properties
Property NameValue
Genbank descriptionCS00-C2-003-011-G11-CT.F Sweet orange bark, greenhouse plant Citrus sinensis cDNA, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>EY685266 ID=EY685266; Name=EY685266; organism=Citrus sinensis; type=EST; length=900bp
CCACGCGTCCGGAATATCCAAATGATCGGCGGATTGTACTCTCGGCTTGG
AATCCTTCTGATCTCAAATTGATGGCGCTTCCACCTTGCCACATGTTTGC
TCAGTTCTATATGGCTAATGGGGAGTTATCATGTCAAATGTATCAGCGCT
CTGCTGATATGGGCCTGGGTGTGCCGTTTAACATTGCTTCATATGCCCTC
TTGACATGCATGATTGCTCATGTTTGTGATCTTGCTCCTGGCGATTTCAT
TCATGTCATTGGTGATGCTCATGTTTATCGCAATCATGTCAGGCCCCTGG
AGGAACAACTTCAGAAACTGCCAAAGCCTTTTCCAATTTTGAAGATCAAT
CCTGAGAAGAAAGATATAGATTCTTTTGTAGCAGAGGATTTCAAACTTAT
AGGTTATGATCCACACCAGAAAATAGAAATGAAAATGGCGGTATAGATCT
CTCTTATAGGTTCAGTAACTCACAGACTCTGGTCCCTCCTAGGCAATAAT
TGCCAAGTTTTGAGGCACCGCTACTGGAGGCATGTATTGACGCAGAGTTT
TATACAAATAGCCAATAAGAATCTGATTGTTGGATAAACTTTTAAATAGT
GCCTATGTAGTAAGCGGGAATCCTTTAGTCCACAAGAGTCGGTGGTTCTA
AGGTGATTTAGGATGTTTGTATTTTCTTGCTTTTCACCTTTCCTTTTCCC
TTGTTTACTGAAAACTATCAAAGTATGTACTTCTGCTCAAAGTCTCAAAT
GCTCTTTTTGAAAAAGATTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAGGGGGGCCGCCCTAAAGGAACCAAGTTTCCTACCCCGT
GGCTTGGGAACCAAAAATTCTTTCAAAAAGGGGCCCTAAATTTCAATTCC
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