EY710335

Overview
NameEY710335
Unique NameEY710335
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length1048
Libraries
Library NameType
Sweet orange fruit, development stadium (2 of 6)cdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of EY710335 vs. ExPASy Swiss-Prot
Match: PGK_BORHD (Phosphoglycerate kinase OS=Borrelia hermsii (strain DAH) GN=pgk PE=3 SV=1)

HSP 1 Score: 114.775 bits (286), Expect = 2.085e-29
Identity = 59/99 (59.60%), Postives = 71/99 (71.72%), Query Frame = 3 
Query:   99 SVSVLKEADLKGKRVFVRVDLNVPLDDNLNITDDNRVRAAVPTIKYLMGHGAKVILSSHLGRPKGV-TPKYSLKPLVPRLSELLGVEVKMANDCFGEEV 392
            S+  +K+ D  GKR  VR D NVPL +  NITDD R+RAA+PTI+YL   GA+V+L SHLGRPKG    KYSL P+  RLSELLG +VKM  DC G+EV
Sbjct:    2 SIKTIKDFDFSGKRALVRCDFNVPLREG-NITDDTRIRAALPTIEYLKSQGARVVLMSHLGRPKGERNLKYSLMPVAKRLSELLGQDVKMLPDCIGDEV 99

HSP 2 Score: 36.1946 bits (82), Expect = 2.085e-29
Identity = 28/92 (30.43%), Postives = 49/92 (53.26%), Query Frame = 1 
Query:  403 VAEIPEGGVLLLENVRFHREEEKNDPKVC*EASFPGRSLW*RKAFGSGPLKPCFPLKGGGLISLKPFLWGWVSFMAKGTKFILVGRLVSNPQ 678
            V+ +  G V+LLEN+RF+R+EE+N      + S  G  ++   AFG+            GL +  P + G++  M K  +F+  G+++ NP+
Sbjct:  103 VSNMKNGDVVLLENIRFYRQEEENCNDFAKKLSQNG-DIFVNDAFGTAHRAHA---STAGLAAYLPAVGGFL--MEKEDEFL--GKILKNPE 186
[Close]
BLAST of EY710335 vs. ExPASy Swiss-Prot
Match: PGK_PROM0 (Phosphoglycerate kinase OS=Prochlorococcus marinus (strain MIT 9301) GN=pgk PE=3 SV=1)

HSP 1 Score: 114.39 bits (285), Expect = 2.369e-29
Identity = 57/101 (56.44%), Postives = 74/101 (73.27%), Query Frame = 3 
Query:   90 AKKSVSVLKEADLKGKRVFVRVDLNVPLDDNLNITDDNRVRAAVPTIKYLMGHGAKVILSSHLGRPKG-VTPKYSLKPLVPRLSELLGVEVKMANDCFGEE 389
            +K S+S L +  L+GK+V VRVD NVPL+++  ITDD R+RAA+PTI+YL+ H AKVIL++H GRPKG V  K  L P+  RLSELLG  V + N C G+E
Sbjct:    2 SKLSLSSLDKTHLEGKKVLVRVDFNVPLNEDGQITDDTRIRAAIPTIEYLVNHSAKVILAAHFGRPKGQVNEKMRLTPVAARLSELLGQSVALTNSCIGDE 102

HSP 2 Score: 31.9574 bits (71), Expect = 2.369e-29
Identity = 15/17 (88.24%), Postives = 15/17 (88.24%), Query Frame = 1 
Query:  427 VLLLENVRFHREEEKND 477
            VLLLENVRF  EEEKND
Sbjct:  115 VLLLENVRFFGEEEKND 131

HSP 3 Score: 24.6386 bits (52), Expect = 2.369e-29
Identity = 14/35 (40.00%), Postives = 21/35 (60.00%), Query Frame = 2 
Query:  458 GRKKRMTPKFAKKLASLADLYGKGRLSDLAH*SHA 562
            G +++   +FA+KLAS AD+Y        AH +HA
Sbjct:  125 GEEEKNDLEFAQKLASHADMYVNDAFG-AAHRAHA 158
[Close]
BLAST of EY710335 vs. ExPASy Swiss-Prot
Match: PGK_MICAN (Phosphoglycerate kinase OS=Microcystis aeruginosa (strain NIES-843) GN=pgk PE=3 SV=1)

HSP 1 Score: 106.686 bits (265), Expect = 2.369e-29
Identity = 61/102 (59.80%), Postives = 71/102 (69.61%), Query Frame = 3 
Query:   93 KKSVSVLKEADLKGKRVFVRVDLNVPLDDNLN-ITDDNRVRAAVPTIKYLMGHGAKVILSSHLGRPKG-VTPKYSLKPLVPRLSELLGVEVKMANDCFGEEV 392
            KK+V+ L EADL GKRV VRVD NVP+D     I+DD R+RAA+PTI+ L+  GAKVIL SH+GRP G V     L P+  RLSELLG EV M  DC GE V
Sbjct:    3 KKTVANLTEADLAGKRVLVRVDFNVPMDKATGAISDDTRIRAALPTIEDLIKKGAKVILCSHMGRPDGQVKENLRLTPVAKRLSELLGQEVIMCPDCIGEGV 104

HSP 2 Score: 36.5798 bits (83), Expect = 2.369e-29
Identity = 15/26 (57.69%), Postives = 20/26 (76.92%), Query Frame = 1 
Query:  403 VAEIPEGGVLLLENVRFHREEEKNDP 480
            ++++  G V LLEN+RFH EEE NDP
Sbjct:  108 ISQMSNGQVALLENLRFHGEEEANDP 133

HSP 3 Score: 27.7202 bits (60), Expect = 2.369e-29
Identity = 16/35 (45.71%), Postives = 20/35 (57.14%), Query Frame = 2 
Query:  458 GRKKRMTPKFAKKLASLADLYGKGRLSDLAH*SHA 562
            G ++   P FAKKLA+ ADLY        AH +HA
Sbjct:  126 GEEEANDPDFAKKLAANADLYVNDAFG-TAHRAHA 159
[Close]
BLAST of EY710335 vs. ExPASy Swiss-Prot
Match: PGK_CYAP8 (Phosphoglycerate kinase OS=Cyanothece sp. (strain PCC 8801) GN=pgk PE=3 SV=1)

HSP 1 Score: 108.997 bits (271), Expect = 2.370e-29
Identity = 61/101 (60.40%), Postives = 69/101 (68.32%), Query Frame = 3 
Query:   93 KKSVSVLKEADLKGKRVFVRVDLNVPLDDNLNITDDNRVRAAVPTIKYLMGHGAKVILSSHLGRPKG-VTPKYSLKPLVPRLSELLGVEVKMANDCFGEEV 392
            KKS+  L  AD+ GKRV VRVD NVPLD    ITDD R+RAA+PTIK L+  GAKVIL SH GRPKG V     L P+  RLSELLG  V M +DC G+ V
Sbjct:    3 KKSIVNLSPADVAGKRVLVRVDFNVPLD-GATITDDTRIRAALPTIKDLIEKGAKVILCSHFGRPKGQVVESMRLTPVATRLSELLGQPVVMCDDCIGDSV 102

HSP 2 Score: 36.5798 bits (83), Expect = 2.370e-29
Identity = 15/27 (55.56%), Postives = 20/27 (74.07%), Query Frame = 1 
Query:  403 VAEIPEGGVLLLENVRFHREEEKNDPK 483
            + ++  G V LLEN+RFH EEE NDP+
Sbjct:  106 INQLQNGQVALLENLRFHAEEEANDPE 132

HSP 3 Score: 25.409 bits (54), Expect = 2.370e-29
Identity = 14/28 (50.00%), Postives = 18/28 (64.29%), Query Frame = 2 
Query:  479 PKFAKKLASLADLYGKGRLSDLAH*SHA 562
            P+FA+KLA+ ADLY        AH +HA
Sbjct:  131 PEFAQKLAANADLYVNDAFG-TAHRAHA 157
[Close]
BLAST of EY710335 vs. ExPASy Swiss-Prot
Match: PGK_PROM5 (Phosphoglycerate kinase OS=Prochlorococcus marinus (strain MIT 9515) GN=pgk PE=3 SV=1)

HSP 1 Score: 114.39 bits (285), Expect = 3.532e-29
Identity = 59/111 (53.15%), Postives = 81/111 (72.97%), Query Frame = 3 
Query:   90 AKKSVSVLKEADLKGKRVFVRVDLNVPLDDNLNITDDNRVRAAVPTIKYLMGHGAKVILSSHLGRPKG-VTPKYSLKPLVPRLSELLGVEVKMANDCFGEE-VEKNGG*NS 416
            +K S+S L ++ L+GK+V VRVD NVPL+D+  ITDD R+RAA+PTIKYL+ + AKVIL++H GRPKG V  +  L P+  RLSE+LG +V +   C GEE +EK+   N+
Sbjct:    2 SKLSLSSLDKSKLEGKKVLVRVDFNVPLNDDGQITDDTRIRAAIPTIKYLINNSAKVILAAHFGRPKGKVNERMRLTPIAARLSEILGKKVNLTESCIGEEALEKSNNLNN 112

HSP 2 Score: 35.8094 bits (81), Expect = 3.532e-29
Identity = 16/19 (84.21%), Postives = 17/19 (89.47%), Query Frame = 1 
Query:  421 GGVLLLENVRFHREEEKND 477
            G VLLLENVRF+ EEEKND
Sbjct:  113 GDVLLLENVRFYEEEEKND 131
[Close]
BLAST of EY710335 vs. ExPASy Swiss-Prot
Match: PGK_FERNB (Phosphoglycerate kinase OS=Fervidobacterium nodosum (strain ATCC 35602 / DSM 5306 / Rt17-B1) GN=pgk PE=3 SV=1)

HSP 1 Score: 111.309 bits (277), Expect = 3.533e-29
Identity = 56/97 (57.73%), Postives = 73/97 (75.26%), Query Frame = 3 
Query:  111 LKEADLKGKRVFVRVDLNVPLDDNLNITDDNRVRAAVPTIKYLMGHGAKVILSSHLGRPK-GVTPKYSLKPLVPRLSELLGVEVKMANDCFGEEVEK 398
            +++ DLKGKRV +RVD NVP+ D + +TDD R+  A+PTIKY++  GAKVIL +HLGRPK G  PKY++KP   RL++LLG EVK     +GEEVEK
Sbjct:    6 IRDVDLKGKRVIMRVDFNVPIKDGV-VTDDTRIVEALPTIKYVLEQGAKVILLTHLGRPKGGPDPKYTVKPAAERLAQLLGKEVKFVPALYGEEVEK 101

HSP 2 Score: 38.891 bits (89), Expect = 3.533e-29
Identity = 18/31 (58.06%), Postives = 24/31 (77.42%), Query Frame = 1 
Query:  385 KKLKKMVAEIPEGGVLLLENVRFHREEEKND 477
            ++++K VAE+ EG VL+LEN RF   EEKND
Sbjct:   97 EEVEKAVAELKEGEVLMLENTRFDPGEEKND 127
[Close]
BLAST of EY710335 vs. ExPASy Swiss-Prot
Match: PGK_GEOLS (Phosphoglycerate kinase OS=Geobacter lovleyi (strain ATCC BAA-1151 / DSM 17278 / SZ) GN=pgk PE=3 SV=1)

HSP 1 Score: 114.005 bits (284), Expect = 3.533e-29
Identity = 56/93 (60.22%), Postives = 69/93 (74.19%), Query Frame = 3 
Query:  123 DLKGKRVFVRVDLNVPLDDNLNITDDNRVRAAVPTIKYLMGHGAKVILSSHLGRPKG-VTPKYSLKPLVPRLSELLGVEVKMANDCFGEEVEK 398
            DLKGK+VF+RVD NVP D+  NIT+D R+  AVPTIKY +  GAKV+L+SHLGRPKG    KY++ P   RLS+LLG +V  A DCFG +V K
Sbjct:   11 DLKGKKVFIRVDFNVPQDEKGNITEDTRIAGAVPTIKYAVEQGAKVVLASHLGRPKGEKKSKYTMAPAAKRLSQLLGKKVMQAPDCFGPDVSK 103

HSP 2 Score: 36.1946 bits (82), Expect = 3.533e-29
Identity = 15/31 (48.39%), Postives = 23/31 (74.19%), Query Frame = 1 
Query:  391 LKKMVAEIPEGGVLLLENVRFHREEEKNDPK 483
            + K++  +  G V++LENVRF+  EEKNDP+
Sbjct:  101 VSKIIDSMKPGDVVMLENVRFYPGEEKNDPE 131
[Close]
BLAST of EY710335 vs. ExPASy Swiss-Prot
Match: PGK_BORRA (Phosphoglycerate kinase OS=Borrelia recurrentis (strain A1) GN=pgk PE=3 SV=1)

HSP 1 Score: 114.005 bits (284), Expect = 3.534e-29
Identity = 57/99 (57.58%), Postives = 72/99 (72.73%), Query Frame = 3 
Query:   99 SVSVLKEADLKGKRVFVRVDLNVPLDDNLNITDDNRVRAAVPTIKYLMGHGAKVILSSHLGRPKG-VTPKYSLKPLVPRLSELLGVEVKMANDCFGEEV 392
            S+  +K+ D  GKR  VR D NVPL +  NITDD R++AA+PTI+YL   GA+V+L SHLGRPKG    +YSL P+  RLSELLG +VKM +DC G+EV
Sbjct:    2 SIRTIKDCDFSGKRALVRCDFNVPLREG-NITDDTRIKAALPTIEYLKSQGARVVLMSHLGRPKGEKNLRYSLMPIARRLSELLGQDVKMLSDCIGDEV 99

HSP 2 Score: 36.1946 bits (82), Expect = 3.534e-29
Identity = 28/92 (30.43%), Postives = 49/92 (53.26%), Query Frame = 1 
Query:  403 VAEIPEGGVLLLENVRFHREEEKNDPKVC*EASFPGRSLW*RKAFGSGPLKPCFPLKGGGLISLKPFLWGWVSFMAKGTKFILVGRLVSNPQ 678
            V+ +  G V+LLENVRF++EEE+N      + S  G  ++   AFG+            G+ S  P + G++  M +  +F+  G+++ NP+
Sbjct:  103 VSCMQNGDVVLLENVRFYKEEEENSDAFAMQLSKSG-DIFVNDAFGTAHRAHA---STSGVASYLPAVGGFL--MEREDEFL--GKILKNPE 186
[Close]
BLAST of EY710335 vs. ExPASy Swiss-Prot
Match: PGK_PROMA (Phosphoglycerate kinase OS=Prochlorococcus marinus GN=pgk PE=3 SV=1)

HSP 1 Score: 104.375 bits (259), Expect = 3.994e-29
Identity = 57/104 (54.81%), Postives = 71/104 (68.27%), Query Frame = 3 
Query:   90 AKKSVSVLKEADLKGKRVFVRVDLNVPLDDNLNITDDNRVRAAVPTIKYLMGHGAKVILSSHLGRPKG-VTPKYSLKPLVPRLSELLGVEVKMANDCFGEEVEK 398
            AK+S+S L   DL GKRV VRVD NVPL++  +ITDD R+RAA+PTI++L+   A+VILS+H GRPKG V     L P+  RLSELLG  V     C G + EK
Sbjct:    2 AKRSLSNLSVEDLCGKRVLVRVDFNVPLNEEGSITDDTRIRAALPTIQHLIEKEARVILSAHFGRPKGKVNEDMRLTPVSQRLSELLGKTVVKTESCIGPDAEK 105

HSP 2 Score: 36.5798 bits (83), Expect = 3.994e-29
Identity = 18/28 (64.29%), Postives = 21/28 (75.00%), Query Frame = 1 
Query:  394 KKMVAEIPEGGVLLLENVRFHREEEKND 477
            +K V E+  G V+LLENVRF  EEEKND
Sbjct:  104 EKKVHEMSNGDVVLLENVRFIGEEEKND 131

HSP 3 Score: 29.261 bits (64), Expect = 3.994e-29
Identity = 16/36 (44.44%), Postives = 23/36 (63.89%), Query Frame = 2 
Query:  455 IGRKKRMTPKFAKKLASLADLYGKGRLSDLAH*SHA 562
            IG +++   +FAKKLASLA++Y        AH +HA
Sbjct:  124 IGEEEKNDSEFAKKLASLAEVYVNDAFG-AAHRAHA 158
[Close]
BLAST of EY710335 vs. ExPASy Swiss-Prot
Match: PGK_ACIBL (Phosphoglycerate kinase OS=Acidobacteria bacterium (strain Ellin345) GN=pgk PE=3 SV=1)

HSP 1 Score: 101.679 bits (252), Expect = 3.994e-29
Identity = 52/103 (50.49%), Postives = 68/103 (66.02%), Query Frame = 3 
Query:  111 LKEADLKGKRVFVRVDLNVPLDDNLNITDDNRVRAAVPTIKYLMGHGAKVILSSHLGRPKG-VTPKYSLKPLVPRLSELL------GVEVKMANDCFGEEVEK 398
            +K+  L  KRVF+RVD NVPLD+N  +TDD R+R  +PTI+Y + HGAK+IL SHLGRPKG   PK SLKP+  RL  +L      G  V  + DC G + ++
Sbjct:    6 IKDLQLSNKRVFMRVDFNVPLDENGRVTDDTRIRETLPTIEYALRHGAKLILCSHLGRPKGKPNPKMSLKPVAERLRVMLDHAISPGQNVGFSPDCIGMQAQE 108

HSP 2 Score: 42.3578 bits (98), Expect = 3.994e-29
Identity = 18/29 (62.07%), Postives = 23/29 (79.31%), Query Frame = 1 
Query:  394 KKMVAEIPEGGVLLLENVRFHREEEKNDP 480
            ++M  ++ +G  LLLENVRFH EEEKNDP
Sbjct:  107 QEMAKQLEKGQALLLENVRFHAEEEKNDP 135

HSP 3 Score: 26.1794 bits (56), Expect = 3.994e-29
Identity = 14/33 (42.42%), Postives = 20/33 (60.61%), Query Frame = 2 
Query:  464 KKRMTPKFAKKLASLADLYGKGRLSDLAH*SHA 562
            +++  P FAK+LASL +LY        AH +HA
Sbjct:  130 EEKNDPAFAKELASLCELYVNDAFGS-AHRAHA 161
The following BLAST results are available for this feature:
BLAST of EY710335 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 500
Match NameE-valueIdentityDescription
PGK_BORHD2.085e-2959.60Phosphoglycerate kinase OS=Borrelia hermsii (strai... [more]
PGK_PROM02.369e-2956.44Phosphoglycerate kinase OS=Prochlorococcus marinus... [more]
PGK_MICAN2.369e-2959.80Phosphoglycerate kinase OS=Microcystis aeruginosa ... [more]
PGK_CYAP82.370e-2960.40Phosphoglycerate kinase OS=Cyanothece sp. (strain ... [more]
PGK_PROM53.532e-2953.15Phosphoglycerate kinase OS=Prochlorococcus marinus... [more]
PGK_FERNB3.533e-2957.73Phosphoglycerate kinase OS=Fervidobacterium nodosu... [more]
PGK_GEOLS3.533e-2960.22Phosphoglycerate kinase OS=Geobacter lovleyi (stra... [more]
PGK_BORRA3.534e-2957.58Phosphoglycerate kinase OS=Borrelia recurrentis (s... [more]
PGK_PROMA3.994e-2954.81Phosphoglycerate kinase OS=Prochlorococcus marinus... [more]
PGK_ACIBL3.994e-2950.49Phosphoglycerate kinase OS=Acidobacteria bacterium... [more]

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Properties
Property NameValue
Genbank descriptionCS00-C3-701-097-F04-CT.F Sweet orange fruit, development stadium (2 of 6) Citrus sinensis cDNA, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>EY710335 ID=EY710335; Name=EY710335; organism=Citrus sinensis; type=EST; length=1048bp
CGTTCACCAATCTTCGATCTCATTCCAAAATCTAACCAAAACCCTTTCTC
GATTTCTCTGATCTCGTGTTTGATCGAATCAAAATGGCCGCCAAGAAGAG
TGTAAGCGTTCTAAAAGAAGCTGATTTGAAGGGAAAGAGAGTGTTCGTAC
GAGTGGATCTGAACGTGCCGTTGGATGACAACTTGAATATCACCGATGAC
AACAGAGTCCGTGCTGCTGTTCCCACTATCAAATACCTGATGGGTCATGG
CGCCAAAGTCATTCTCTCCAGTCACTTGGGGCGTCCAAAGGGTGTTACAC
CCAAGTACAGCTTGAAACCTCTTGTGCCAAGGCTTTCTGAACTTCTTGGA
GTTGAGGTTAAAATGGCAAACGACTGCTTTGGTGAAGAAGTTGAAAAAAA
TGGTGGCTGAAATTCCCGAAGGGGGCGTCTTGCTCCTCGAAAATGTGAGG
TTCCATAGGGAGGAAGAAAAGAATGACCCCAAAGTTTGCTAAGAAGCTAG
CTTCCCTGGCAGATCTCTATGGTAAAGGAAGGCTTTCGGATCTGGCCCAT
TAAAGCCATGCTTTCCCCTGAAGGGTGGTGGGCTAATTTCTTTGAAACCC
TTTCTGTGGGGCTGGGTTTCCTTTATGGCAAAAGGGACTAAATTTATCTT
GGTTGGGAGGCTGGTTTCAAATCCCCAAAAAAACCATTTTGGCGGCCCTT
TGTTGGGGGGGTTTAAAAAAGGGGGAAAACTAAAAAAATGGGGGTTTATT
GGATTCCCTTTTTGGGGAAAAAGGGTGAACATTTTTATTTTGCCCGGGGG
GAAAGAATATAATTTTTATCACCTTTATCACAGGGCCCAAAGGGAACAAC
CTTTGGTTGGGTATCATCCCCTTGTGTGAAGGGAAAAAAAAAAAGTTTTT
GTTTACTCCCCCACATTTGGCCCTTTTATGGGAAAAAAGAGGGGACAGAT
TCTTAAAAAGAGGGGGGTGTGGTCTCTATCTATGTATACTCCCCTCTCTA
AGTATGTGGTGTGTTGTTTTTTTTTGTTTTTTGTACGATCAAAAAAAT
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