EY710335

Overview
NameEY710335
Unique NameEY710335
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length1048
Libraries
Library NameType
Sweet orange fruit, development stadium (2 of 6)cdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of EY710335 vs. ExPASy Swiss-Prot
Match: PGK_BACME (Phosphoglycerate kinase OS=Bacillus megaterium GN=pgk PE=3 SV=1)

HSP 1 Score: 108.612 bits (270), Expect = 4.601e-29
Identity = 56/97 (57.73%), Postives = 72/97 (74.23%), Query Frame = 3 
Query:  111 LKEADLKGKRVFVRVDLNVPLDDNLNITDDNRVRAAVPTIKYLMGHGAKVILSSHLGRPKG-VTPKYSLKPLVPRLSELLGVEVKMANDCFGEEVEK 398
            LK+ D+KGKRVF RVD NVP+ D   +TD+ R+RAA+PTI+YL+  GAKVIL+SHLGRPKG V  +  L  +  RL  LLG +V  A++ FGEEV+K
Sbjct:    6 LKDIDVKGKRVFCRVDFNVPMKDG-KVTDETRIRAAIPTIQYLVEQGAKVILASHLGRPKGEVVEELRLNAVAERLQALLGKDVAKADEAFGEEVKK 101

HSP 2 Score: 41.2022 bits (95), Expect = 4.601e-29
Identity = 19/37 (51.35%), Postives = 28/37 (75.68%), Query Frame = 1 
Query:  376 ALVKKLKKMVAEIPEGGVLLLENVRFHREEEKNDPKV 486
            A  +++KK +  + EG VL+LENVRF+  EEKNDP++
Sbjct:   94 AFGEEVKKTIDGMSEGDVLVLENVRFYPGEEKNDPEL 130
[Close]
BLAST of EY710335 vs. ExPASy Swiss-Prot
Match: PGK_BORDL (Phosphoglycerate kinase OS=Borrelia duttonii (strain Ly) GN=pgk PE=3 SV=1)

HSP 1 Score: 113.235 bits (282), Expect = 5.991e-29
Identity = 57/99 (57.58%), Postives = 71/99 (71.72%), Query Frame = 3 
Query:   99 SVSVLKEADLKGKRVFVRVDLNVPLDDNLNITDDNRVRAAVPTIKYLMGHGAKVILSSHLGRPKG-VTPKYSLKPLVPRLSELLGVEVKMANDCFGEEV 392
            S+  +K+ D  GKR  VR D NVPL +  NITDD R++AA+PTI+YL   GA+V+L SHLGRPKG    +YSL P+  RLSELLG  VKM +DC G+EV
Sbjct:    2 SIRTIKDCDFSGKRALVRCDFNVPLREG-NITDDTRIKAALPTIEYLKSQGARVVLMSHLGRPKGEKNLRYSLMPIARRLSELLGQNVKMLSDCIGDEV 99

HSP 2 Score: 36.1946 bits (82), Expect = 5.991e-29
Identity = 28/92 (30.43%), Postives = 49/92 (53.26%), Query Frame = 1 
Query:  403 VAEIPEGGVLLLENVRFHREEEKNDPKVC*EASFPGRSLW*RKAFGSGPLKPCFPLKGGGLISLKPFLWGWVSFMAKGTKFILVGRLVSNPQ 678
            V+ +  G V+LLENVRF++EEE+N      + S  G  ++   AFG+            G+ S  P + G++  M +  +F+  G+++ NP+
Sbjct:  103 VSCMQNGDVVLLENVRFYKEEEENSDAFAMQLSKSG-DIFVNDAFGTAHRAHA---STSGVASYLPAVGGFL--MEREDEFL--GKILKNPE 186
[Close]
BLAST of EY710335 vs. ExPASy Swiss-Prot
Match: PGK_MOOTA (Phosphoglycerate kinase OS=Moorella thermoacetica (strain ATCC 39073) GN=pgk PE=3 SV=1)

HSP 1 Score: 103.219 bits (256), Expect = 6.738e-29
Identity = 54/96 (56.25%), Postives = 67/96 (69.79%), Query Frame = 3 
Query:  111 LKEADLKGKRVFVRVDLNVPLDDNLNITDDNRVRAAVPTIKYLMGHGAKVILSSHLGRPKG-VTPKYSLKPLVPRLSELLGVEVKMANDCFGEEVE 395
            LK+ +L  KRV VRVD NVPL+    +TD+ R+RAA+PTI+YL+ HGA+VIL SHLGRPKG V  +  L P+   L  LLG +V   NDC G EVE
Sbjct:    6 LKDLELNNKRVLVRVDFNVPLEAG-RVTDNTRIRAALPTIEYLLDHGARVILMSHLGRPKGKVKEELRLDPVARELESLLGRQVHKVNDCVGPEVE 100

HSP 2 Score: 40.0466 bits (92), Expect = 6.738e-29
Identity = 18/31 (58.06%), Postives = 23/31 (74.19%), Query Frame = 1 
Query:  388 KLKKMVAEIPEGGVLLLENVRFHREEEKNDP 480
            +++   A +  G VLLLEN+RFH EEEKNDP
Sbjct:   98 EVEAAAAALKPGEVLLLENLRFHPEEEKNDP 128

HSP 3 Score: 26.1794 bits (56), Expect = 6.738e-29
Identity = 14/33 (42.42%), Postives = 21/33 (63.64%), Query Frame = 2 
Query:  464 KKRMTPKFAKKLASLADLYGKGRLSDLAH*SHA 562
            +++  P FA++LASLAD+Y        AH +HA
Sbjct:  123 EEKNDPGFARQLASLADVYVNDAFG-AAHRAHA 154
[Close]
BLAST of EY710335 vs. ExPASy Swiss-Prot
Match: PGK_ANASK (Phosphoglycerate kinase OS=Anaeromyxobacter sp. (strain K) GN=pgk PE=3 SV=1)

HSP 1 Score: 103.219 bits (256), Expect = 7.797e-29
Identity = 54/95 (56.84%), Postives = 69/95 (72.63%), Query Frame = 3 
Query:  123 DLKGKRVFVRVDLNVPLDDNLNITDDNRVRAAVPTIKYLMGHGAKVILSSHLGRPKGV---TPKYSLKPLVPRLSELLGVEVKMANDCFGEEVEK 398
            DL GKRVF+RVD NVPLD    +TDD R+RAA+PTI++ +   AKVIL+SHLGRPKG      K +L+P   RLSELL  +V +A+DC G+ V+K
Sbjct:   10 DLAGKRVFIRVDFNVPLDPQGRVTDDARIRAALPTIRHAIQAKAKVILASHLGRPKGKPDDRQKLTLEPAAVRLSELLSQDVILADDCVGDGVKK 104

HSP 2 Score: 45.8246 bits (107), Expect = 7.797e-29
Identity = 24/51 (47.06%), Postives = 33/51 (64.71%), Query Frame = 1 
Query:  391 LKKMVAEIPEGGVLLLENVRFHREEEKNDPKVC*EASFPGRSLW*RKAFGS 543
            +KK+V ++ +G VLLLEN+RFH EEEKND     E +     +W   AFG+
Sbjct:  102 VKKLVRDLKDGHVLLLENLRFHPEEEKNDEAFARELASLA-DVWVNDAFGT 151
[Close]
BLAST of EY710335 vs. ExPASy Swiss-Prot
Match: PGK_ANADE (Phosphoglycerate kinase OS=Anaeromyxobacter dehalogenans (strain 2CP-C) GN=pgk PE=3 SV=1)

HSP 1 Score: 103.219 bits (256), Expect = 7.797e-29
Identity = 54/95 (56.84%), Postives = 69/95 (72.63%), Query Frame = 3 
Query:  123 DLKGKRVFVRVDLNVPLDDNLNITDDNRVRAAVPTIKYLMGHGAKVILSSHLGRPKGV---TPKYSLKPLVPRLSELLGVEVKMANDCFGEEVEK 398
            DL GKRVF+RVD NVPLD    +TDD R+RAA+PTI++ +   AKVIL+SHLGRPKG      K +L+P   RLSELL  +V +A+DC G+ V+K
Sbjct:   10 DLAGKRVFIRVDFNVPLDPQGKVTDDARIRAALPTIRHAIQAKAKVILASHLGRPKGKPDDRTKLTLEPAAVRLSELLSQDVILADDCVGDGVKK 104

HSP 2 Score: 45.8246 bits (107), Expect = 7.797e-29
Identity = 24/51 (47.06%), Postives = 33/51 (64.71%), Query Frame = 1 
Query:  391 LKKMVAEIPEGGVLLLENVRFHREEEKNDPKVC*EASFPGRSLW*RKAFGS 543
            +KK+V ++ +G VLLLEN+RFH EEEKND     E +     +W   AFG+
Sbjct:  102 VKKLVRDLKDGHVLLLENLRFHPEEEKNDEAFARELASLA-DVWVNDAFGT 151
[Close]
BLAST of EY710335 vs. ExPASy Swiss-Prot
Match: PGK_BORT9 (Phosphoglycerate kinase OS=Borrelia turicatae (strain 91E135) GN=pgk PE=3 SV=1)

HSP 1 Score: 115.931 bits (289), Expect = 7.799e-29
Identity = 60/99 (60.61%), Postives = 71/99 (71.72%), Query Frame = 3 
Query:   99 SVSVLKEADLKGKRVFVRVDLNVPLDDNLNITDDNRVRAAVPTIKYLMGHGAKVILSSHLGRPKG-VTPKYSLKPLVPRLSELLGVEVKMANDCFGEEV 392
            S+  +K+ D  GKR  VR D NVPL +  NITDD R+RAA+PTI+YL   GA+VIL SHLGRPKG    KYSL P+  RLSELLG +VKM  DC G+EV
Sbjct:    2 SIKTIKDCDFAGKRALVRCDFNVPLKEG-NITDDTRIRAALPTIEYLKSQGARVILMSHLGRPKGEKNLKYSLMPVAKRLSELLGQDVKMLPDCIGDEV 99

HSP 2 Score: 33.113 bits (74), Expect = 7.799e-29
Identity = 27/92 (29.35%), Postives = 49/92 (53.26%), Query Frame = 1 
Query:  403 VAEIPEGGVLLLENVRFHREEEKNDPKVC*EASFPGRSLW*RKAFGSGPLKPCFPLKGGGLISLKPFLWGWVSFMAKGTKFILVGRLVSNPQ 678
            V+ +  G ++LLEN+RF++EEE+N      + S  G  ++   AFG+            GL +  P + G++  M K  +F+  G+++ NP+
Sbjct:  103 VSCMKNGDIVLLENLRFYKEEEENCDAFVRKLSQNG-DIFVNDAFGTAHRAHA---STAGLAAYLPAVGGFL--MEKEDEFL--GKILRNPE 186
[Close]
BLAST of EY710335 vs. ExPASy Swiss-Prot
Match: PGK_SOLUE (Phosphoglycerate kinase OS=Solibacter usitatus (strain Ellin6076) GN=pgk PE=3 SV=1)

HSP 1 Score: 110.153 bits (274), Expect = 7.799e-29
Identity = 56/100 (56.00%), Postives = 73/100 (73.00%), Query Frame = 3 
Query:  111 LKEADLKGKRVFVRVDLNVPLDDN----LNITDDNRVRAAVPTIKYLMGHGAKVILSSHLGRPKG-VTPKYSLKPLVPRLSELLGVEVKMANDCFGEEVE 395
            +++ DLKGKRVF+RVD NVPL  N    + IT D R++A++PTI+Y + HGA VIL+SHLGRPKG    + SLKP+  RL ++L V VKMA DC G EV+
Sbjct:    6 IRDLDLKGKRVFIRVDFNVPLQKNDQGAMEITSDKRIKASLPTIQYALEHGAAVILASHLGRPKGKPNTEMSLKPVAARLEQVLKVPVKMAPDCVGPEVQ 105

HSP 2 Score: 38.891 bits (89), Expect = 7.799e-29
Identity = 17/20 (85.00%), Postives = 18/20 (90.00%), Query Frame = 1 
Query:  421 GGVLLLENVRFHREEEKNDP 480
            G VLLLEN+RFH EEEKNDP
Sbjct:  112 GEVLLLENLRFHAEEEKNDP 131
[Close]
BLAST of EY710335 vs. ExPASy Swiss-Prot
Match: PGK_THEEB (Phosphoglycerate kinase OS=Thermosynechococcus elongatus (strain BP-1) GN=pgk PE=3 SV=1)

HSP 1 Score: 112.849 bits (281), Expect = 1.015e-28
Identity = 59/101 (58.42%), Postives = 72/101 (71.29%), Query Frame = 3 
Query:   90 AKKSVSVLKEADLKGKRVFVRVDLNVPLDDNLNITDDNRVRAAVPTIKYLMGHGAKVILSSHLGRPKGVTPKYSLKPLVPRLSELLGVEVKMANDCFGEEV 392
            +KKSV+ L  ADL+GKRV VRVD NVP+D+   ITD+ R+RAA+PTI+ L+  GAKVIL SH GRPKGV  K  L P+  RLSELL   V   +DC G+ V
Sbjct:    2 SKKSVAQLSAADLEGKRVLVRVDFNVPVDEKGVITDETRIRAALPTIQDLISKGAKVILVSHFGRPKGVDEKLRLTPVAQRLSELLHKPVAKLDDCIGDAV 102

HSP 2 Score: 35.8094 bits (81), Expect = 1.015e-28
Identity = 16/21 (76.19%), Postives = 17/21 (80.95%), Query Frame = 1 
Query:  421 GGVLLLENVRFHREEEKNDPK 483
            G V LLENVRFH  EEKNDP+
Sbjct:  112 GDVCLLENVRFHPGEEKNDPE 132
[Close]
BLAST of EY710335 vs. ExPASy Swiss-Prot
Match: PGK_CHLP8 (Phosphoglycerate kinase OS=Chlorobaculum parvum (strain NCIB 8327) GN=pgk PE=3 SV=1)

HSP 1 Score: 110.153 bits (274), Expect = 1.015e-28
Identity = 55/95 (57.89%), Postives = 69/95 (72.63%), Query Frame = 3 
Query:  111 LKEADLKGKRVFVRVDLNVPLDDNLNITDDNRVRAAVPTIKYLMGHGAKVILSSHLGRPKG-VTPKYSLKPLVPRLSELLGVEVKMANDCFGEEV 392
            L +  L+GKRV +RVD NVPLD + NITDD R+  A+P+I+ ++  G ++IL SHLGRPKG V P +SL P   RLSELLG+ V MANDC G EV
Sbjct:    6 LSDISLQGKRVLMRVDFNVPLDQDRNITDDKRIVGALPSIRKVIEEGGRLILMSHLGRPKGKVNPAFSLAPAAKRLSELLGMPVTMANDCIGTEV 100

HSP 2 Score: 38.5058 bits (88), Expect = 1.015e-28
Identity = 20/47 (42.55%), Postives = 29/47 (61.70%), Query Frame = 1 
Query:  403 VAEIPEGGVLLLENVRFHREEEKNDPKVC*EASFPGRSLW*RKAFGS 543
            V  + +G V++LEN+RFH EEE NDP    E +  G  ++   AFG+
Sbjct:  104 VLALQDGEVMMLENLRFHPEEEANDPDFARELASMG-EIYVNDAFGT 149
[Close]
BLAST of EY710335 vs. ExPASy Swiss-Prot
Match: PGK_BACCN (Phosphoglycerate kinase OS=Bacillus cereus subsp. cytotoxis (strain NVH 391-98) GN=pgk PE=3 SV=1)

HSP 1 Score: 109.383 bits (272), Expect = 1.015e-28
Identity = 59/103 (57.28%), Postives = 76/103 (73.79%), Query Frame = 3 
Query:   93 KKSVSVLKEADLKGKRVFVRVDLNVPLDDNLNITDDNRVRAAVPTIKYLMGHGAKVILSSHLGRPKG-VTPKYSLKPLVPRLSELLGVEVKMANDCFGEEVEK 398
            KKS+   ++ DLKGKRVF RVD NVP+ D   ITD+ R+RAA+PTI+YL+  GAKVIL+SHLGRPKG V  +  L P+  RL ELLG +VK A++ FG  V++
Sbjct:    3 KKSI---RDVDLKGKRVFCRVDFNVPMKDG-KITDETRIRAALPTIQYLVEQGAKVILASHLGRPKGQVVEEMRLTPVAVRLGELLGKDVKKADEAFGPAVQE 101

HSP 2 Score: 39.2762 bits (90), Expect = 1.015e-28
Identity = 18/35 (51.43%), Postives = 27/35 (77.14%), Query Frame = 1 
Query:  391 LKKMVAEIPEGGVLLLENVRFHREEEKNDPKVC*E 495
            +++MVA + EG +L+LENVRF+  EEKND ++  E
Sbjct:   99 VQEMVAAMNEGDILVLENVRFYAGEEKNDAELAKE 133
The following BLAST results are available for this feature:
BLAST of EY710335 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 500
Match NameE-valueIdentityDescription
PGK_BACME4.601e-2957.73Phosphoglycerate kinase OS=Bacillus megaterium GN=... [more]
PGK_BORDL5.991e-2957.58Phosphoglycerate kinase OS=Borrelia duttonii (stra... [more]
PGK_MOOTA6.738e-2956.25Phosphoglycerate kinase OS=Moorella thermoacetica ... [more]
PGK_ANASK7.797e-2956.84Phosphoglycerate kinase OS=Anaeromyxobacter sp. (s... [more]
PGK_ANADE7.797e-2956.84Phosphoglycerate kinase OS=Anaeromyxobacter dehalo... [more]
PGK_BORT97.799e-2960.61Phosphoglycerate kinase OS=Borrelia turicatae (str... [more]
PGK_SOLUE7.799e-2956.00Phosphoglycerate kinase OS=Solibacter usitatus (st... [more]
PGK_THEEB1.015e-2858.42Phosphoglycerate kinase OS=Thermosynechococcus elo... [more]
PGK_CHLP81.015e-2857.89Phosphoglycerate kinase OS=Chlorobaculum parvum (s... [more]
PGK_BACCN1.015e-2857.28Phosphoglycerate kinase OS=Bacillus cereus subsp. ... [more]

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Properties
Property NameValue
Genbank descriptionCS00-C3-701-097-F04-CT.F Sweet orange fruit, development stadium (2 of 6) Citrus sinensis cDNA, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>EY710335 ID=EY710335; Name=EY710335; organism=Citrus sinensis; type=EST; length=1048bp
CGTTCACCAATCTTCGATCTCATTCCAAAATCTAACCAAAACCCTTTCTC
GATTTCTCTGATCTCGTGTTTGATCGAATCAAAATGGCCGCCAAGAAGAG
TGTAAGCGTTCTAAAAGAAGCTGATTTGAAGGGAAAGAGAGTGTTCGTAC
GAGTGGATCTGAACGTGCCGTTGGATGACAACTTGAATATCACCGATGAC
AACAGAGTCCGTGCTGCTGTTCCCACTATCAAATACCTGATGGGTCATGG
CGCCAAAGTCATTCTCTCCAGTCACTTGGGGCGTCCAAAGGGTGTTACAC
CCAAGTACAGCTTGAAACCTCTTGTGCCAAGGCTTTCTGAACTTCTTGGA
GTTGAGGTTAAAATGGCAAACGACTGCTTTGGTGAAGAAGTTGAAAAAAA
TGGTGGCTGAAATTCCCGAAGGGGGCGTCTTGCTCCTCGAAAATGTGAGG
TTCCATAGGGAGGAAGAAAAGAATGACCCCAAAGTTTGCTAAGAAGCTAG
CTTCCCTGGCAGATCTCTATGGTAAAGGAAGGCTTTCGGATCTGGCCCAT
TAAAGCCATGCTTTCCCCTGAAGGGTGGTGGGCTAATTTCTTTGAAACCC
TTTCTGTGGGGCTGGGTTTCCTTTATGGCAAAAGGGACTAAATTTATCTT
GGTTGGGAGGCTGGTTTCAAATCCCCAAAAAAACCATTTTGGCGGCCCTT
TGTTGGGGGGGTTTAAAAAAGGGGGAAAACTAAAAAAATGGGGGTTTATT
GGATTCCCTTTTTGGGGAAAAAGGGTGAACATTTTTATTTTGCCCGGGGG
GAAAGAATATAATTTTTATCACCTTTATCACAGGGCCCAAAGGGAACAAC
CTTTGGTTGGGTATCATCCCCTTGTGTGAAGGGAAAAAAAAAAAGTTTTT
GTTTACTCCCCCACATTTGGCCCTTTTATGGGAAAAAAGAGGGGACAGAT
TCTTAAAAAGAGGGGGGTGTGGTCTCTATCTATGTATACTCCCCTCTCTA
AGTATGTGGTGTGTTGTTTTTTTTTGTTTTTTGTACGATCAAAAAAAT
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