EY710335

Overview
NameEY710335
Unique NameEY710335
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length1048
Libraries
Library NameType
Sweet orange fruit, development stadium (2 of 6)cdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of EY710335 vs. ExPASy Swiss-Prot
Match: PGK_CAMLR (Phosphoglycerate kinase OS=Campylobacter lari (strain RM2100 / D67 / ATCC BAA-1060) GN=pgk PE=3 SV=1)

HSP 1 Score: 112.849 bits (281), Expect = 1.474e-28
Identity = 52/100 (52.00%), Postives = 73/100 (73.00%), Query Frame = 3 
Query:   99 SVSVLKEADLKGKRVFVRVDLNVPLDDNLNITDDNRVRAAVPTIKYLMGHGAKVILSSHLGRPKGVTPKYSLKPLVPRLSELLGVEVKMANDCFGEEVEK 398
            S+  +K+ DL  K+VF+R D NVP D+ LNITDD R+R+A+PTI+Y + +G  VIL+SHLGRPK +  KYSL+P+  RL+ L+  +V MA D  GE+ +K
Sbjct:    3 SILSIKDIDLAKKKVFIRCDFNVPQDEFLNITDDRRIRSAIPTIRYCLDNGCAVILASHLGRPKEIASKYSLEPVAKRLARLMAKDVIMAKDVIGEDAKK 102

HSP 2 Score: 33.113 bits (74), Expect = 1.474e-28
Identity = 15/34 (44.12%), Postives = 21/34 (61.76%), Query Frame = 1 
Query:  394 KKMVAEIPEGGVLLLENVRFHREEEKNDPKVC*E 495
            KK  +E+    +LLLEN+RF + E KND  +  E
Sbjct:  101 KKKASELKSSEILLLENLRFEKGETKNDENLAKE 134

HSP 3 Score: 22.3274 bits (46), Expect = 1.474e-28
Identity = 17/46 (36.96%), Postives = 24/46 (52.17%), Query Frame = 2 
Query:  488 AKKLASLADLYGKGRLSDLAH*SHAFP*RVVG*FL*NPFCGAGFPL 625
            AK+LAS+AD+Y       + H +HA     +  +  N   GAGF L
Sbjct:  132 AKELASMADVYINDAFG-VCHRAHASV-EAITKYFDNTHKGAGFLL 175
[Close]
BLAST of EY710335 vs. ExPASy Swiss-Prot
Match: PGK_BACCZ (Phosphoglycerate kinase OS=Bacillus cereus (strain ZK / E33L) GN=pgk PE=3 SV=1)

HSP 1 Score: 108.227 bits (269), Expect = 1.721e-28
Identity = 58/103 (56.31%), Postives = 76/103 (73.79%), Query Frame = 3 
Query:   93 KKSVSVLKEADLKGKRVFVRVDLNVPLDDNLNITDDNRVRAAVPTIKYLMGHGAKVILSSHLGRPKG-VTPKYSLKPLVPRLSELLGVEVKMANDCFGEEVEK 398
            KKS+   ++ DLKGKRVF RVD NVP+ +   ITD+ R+RAA+PTI+YL+  GAKVIL+SHLGRPKG V  +  L P+  RL ELLG +VK A++ FG  V++
Sbjct:    3 KKSI---RDVDLKGKRVFCRVDFNVPMKEG-KITDETRIRAALPTIQYLVEQGAKVILASHLGRPKGQVVEEMRLTPVAARLGELLGKDVKKADEAFGPAVQE 101

HSP 2 Score: 39.6614 bits (91), Expect = 1.721e-28
Identity = 19/35 (54.29%), Postives = 27/35 (77.14%), Query Frame = 1 
Query:  391 LKKMVAEIPEGGVLLLENVRFHREEEKNDPKVC*E 495
            +++MVA + EG VL+LENVRF+  EEKND ++  E
Sbjct:   99 VQEMVAAMNEGDVLVLENVRFYAGEEKNDAELAKE 133
[Close]
BLAST of EY710335 vs. ExPASy Swiss-Prot
Match: PGK_BACC1 (Phosphoglycerate kinase OS=Bacillus cereus (strain ATCC 10987) GN=pgk PE=3 SV=1)

HSP 1 Score: 108.227 bits (269), Expect = 1.721e-28
Identity = 58/103 (56.31%), Postives = 76/103 (73.79%), Query Frame = 3 
Query:   93 KKSVSVLKEADLKGKRVFVRVDLNVPLDDNLNITDDNRVRAAVPTIKYLMGHGAKVILSSHLGRPKG-VTPKYSLKPLVPRLSELLGVEVKMANDCFGEEVEK 398
            KKS+   ++ DLKGKRVF RVD NVP+ +   ITD+ R+RAA+PTI+YL+  GAKVIL+SHLGRPKG V  +  L P+  RL ELLG +VK A++ FG  V++
Sbjct:    3 KKSI---RDVDLKGKRVFCRVDFNVPMKEG-KITDETRIRAALPTIQYLVEQGAKVILASHLGRPKGQVVEEMRLTPVAARLGELLGKDVKKADEAFGPAVQE 101

HSP 2 Score: 39.6614 bits (91), Expect = 1.721e-28
Identity = 19/35 (54.29%), Postives = 27/35 (77.14%), Query Frame = 1 
Query:  391 LKKMVAEIPEGGVLLLENVRFHREEEKNDPKVC*E 495
            +++MVA + EG VL+LENVRF+  EEKND ++  E
Sbjct:   99 VQEMVAAMNEGDVLVLENVRFYAGEEKNDAELAKE 133
[Close]
BLAST of EY710335 vs. ExPASy Swiss-Prot
Match: PGK_BACSU (Phosphoglycerate kinase OS=Bacillus subtilis GN=pgk PE=1 SV=3)

HSP 1 Score: 106.686 bits (265), Expect = 1.721e-28
Identity = 55/96 (57.29%), Postives = 71/96 (73.96%), Query Frame = 3 
Query:  111 LKEADLKGKRVFVRVDLNVPLDDNLNITDDNRVRAAVPTIKYLMGHGAKVILSSHLGRPKG-VTPKYSLKPLVPRLSELLGVEVKMANDCFGEEVE 395
            LK+ D+KGK VF RVD NVP+ D   +TDD R+RAA+PTIK+L   GAKV+L+SHLGRPKG V  +  L P+  RL ELLG EVK A++ +G+ V+
Sbjct:    6 LKDIDVKGKVVFCRVDFNVPMKDG-EVTDDTRIRAALPTIKHLADQGAKVLLASHLGRPKGEVVEELRLTPVAARLGELLGKEVKKADEAYGDAVK 100

HSP 2 Score: 41.2022 bits (95), Expect = 1.721e-28
Identity = 31/97 (31.96%), Postives = 53/97 (54.64%), Query Frame = 1 
Query:  391 LKKMVAEIPEGGVLLLENVRFHREEEKNDPKVC*EASFPGRSLW*RKAFGSGPLKPCFPLKGGGLISLKPFLWGWVSFMAKGTKFILVGRLVSNPQK 681
            +K  ++E+ +G VL+LENVRF+  EEKNDP++  +A      ++   AFG+            G+    P + G++  M K  +  ++G+ VSNP +
Sbjct:   99 VKAQISEMKDGDVLVLENVRFYPGEEKNDPELA-KAFAELADVYVNDAFGAAHRAHA---STAGIAEHLPAVAGFL--MEK--ELDVLGKAVSNPDR 187
[Close]
BLAST of EY710335 vs. ExPASy Swiss-Prot
Match: PGK_PROMP (Phosphoglycerate kinase OS=Prochlorococcus marinus subsp. pastoris (strain CCMP1986 / MED4) GN=pgk PE=3 SV=1)

HSP 1 Score: 109.768 bits (273), Expect = 1.913e-28
Identity = 55/101 (54.46%), Postives = 74/101 (73.27%), Query Frame = 3 
Query:   90 AKKSVSVLKEADLKGKRVFVRVDLNVPLDDNLNITDDNRVRAAVPTIKYLMGHGAKVILSSHLGRPKG-VTPKYSLKPLVPRLSELLGVEVKMANDCFGEE 389
            +K S+S L ++ L+GK+V VRVD NVPL+D+  ITDD R+RAA+PTIKYL+ + AKVIL++H GRPKG V  K  L P+  RLS++L  +V +   C GEE
Sbjct:    2 SKLSLSSLDKSKLEGKKVLVRVDFNVPLNDSGQITDDTRIRAAIPTIKYLINNSAKVILAAHFGRPKGKVNEKMRLTPVAARLSDILEQKVNLTESCIGEE 102

HSP 2 Score: 35.4242 bits (80), Expect = 1.913e-28
Identity = 16/19 (84.21%), Postives = 17/19 (89.47%), Query Frame = 1 
Query:  421 GGVLLLENVRFHREEEKND 477
            G VLLLENVRF+ EEEKND
Sbjct:  113 GEVLLLENVRFYEEEEKND 131

HSP 3 Score: 22.7126 bits (47), Expect = 1.913e-28
Identity = 27/70 (38.57%), Postives = 35/70 (50.00%), Query Frame = 2 
Query:  485 FAKKLASLADLYGKGRLSDLAH*SHAFP*RVVG*FL*NPFCGAGFPLWQKGLNLSWLGGWFQIPKKTILA 694
            FAK LAS AD+Y        AH +HA   + V  FL  P   AGF L ++   L +L G    PK+ + A
Sbjct:  134 FAKNLASHADMYVNDAFG-AAHRAHAST-QGVTKFL-EP-AVAGFLLEKE---LKYLQGAIDAPKRPLAA 196
[Close]
BLAST of EY710335 vs. ExPASy Swiss-Prot
Match: PGK_HYDS0 (Phosphoglycerate kinase OS=Hydrogenobaculum sp. (strain Y04AAS1) GN=pgk PE=3 SV=1)

HSP 1 Score: 114.775 bits (286), Expect = 1.914e-28
Identity = 56/95 (58.95%), Postives = 71/95 (74.74%), Query Frame = 3 
Query:  111 LKEADLKGKRVFVRVDLNVPLDDNLNITDDNRVRAAVPTIKYLMGHGAKVILSSHLGRPKGVTPKYSLKPLVPRLSELLGVEVKMANDCFGEEVE 395
            +++ DLKGKRV VRVD NVP+D+  NI DD R+RAA+PTI+YL+   A VIL SHLGRPKG   KYSLKP+  RLS  +  +V MA DC G+++E
Sbjct:    7 IRDIDLKGKRVLVRVDFNVPIDNQGNIEDDARIRAAIPTIEYLLDAKAIVILMSHLGRPKGFDEKYSLKPVAKRLSRYIHKDVVMAPDCIGKDME 101

HSP 2 Score: 31.5722 bits (70), Expect = 1.914e-28
Identity = 13/31 (41.94%), Postives = 21/31 (67.74%), Query Frame = 1 
Query:  385 KKLKKMVAEIPEGGVLLLENVRFHREEEKND 477
            K ++ +V       V++LEN+RFH+EEE+ D
Sbjct:   98 KDMENIVKNAKSEDVIMLENLRFHKEEEECD 128

HSP 3 Score: 21.557 bits (44), Expect = 1.914e-28
Identity = 8/13 (61.54%), Postives = 12/13 (92.31%), Query Frame = 2 
Query:  482 KFAKKLASLADLY 520
            +F+KKLASL ++Y
Sbjct:  130 EFSKKLASLGEVY 142
[Close]
BLAST of EY710335 vs. ExPASy Swiss-Prot
Match: PGK_ANAD2 (Phosphoglycerate kinase OS=Anaeromyxobacter dehalogenans (strain 2CP-1 / ATCC BAA-258) GN=pgk PE=3 SV=1)

HSP 1 Score: 101.679 bits (252), Expect = 2.240e-28
Identity = 53/95 (55.79%), Postives = 69/95 (72.63%), Query Frame = 3 
Query:  123 DLKGKRVFVRVDLNVPLDDNLNITDDNRVRAAVPTIKYLMGHGAKVILSSHLGRPKGV---TPKYSLKPLVPRLSELLGVEVKMANDCFGEEVEK 398
            +L GKRVF+RVD NVPLD    +TDD R+RAA+PTI++ +   AKVIL+SHLGRPKG      K +L+P   RLSELL  +V +A+DC G+ V+K
Sbjct:   10 ELAGKRVFIRVDFNVPLDPQGRVTDDARIRAALPTIRHAIQAKAKVILASHLGRPKGKPEDRQKLTLEPAAVRLSELLSQDVILADDCVGDGVKK 104

HSP 2 Score: 45.8246 bits (107), Expect = 2.240e-28
Identity = 24/51 (47.06%), Postives = 33/51 (64.71%), Query Frame = 1 
Query:  391 LKKMVAEIPEGGVLLLENVRFHREEEKNDPKVC*EASFPGRSLW*RKAFGS 543
            +KK+V ++ +G VLLLEN+RFH EEEKND     E +     +W   AFG+
Sbjct:  102 VKKLVRDLKDGHVLLLENLRFHPEEEKNDEAFARELASLA-DVWVNDAFGT 151
[Close]
BLAST of EY710335 vs. ExPASy Swiss-Prot
Match: PGK_PORGI (Phosphoglycerate kinase OS=Porphyromonas gingivalis GN=pgk PE=3 SV=1)

HSP 1 Score: 115.161 bits (287), Expect = 3.220e-28
Identity = 50/93 (53.76%), Postives = 71/93 (76.34%), Query Frame = 3 
Query:  111 LKEADLKGKRVFVRVDLNVPLDDNLNITDDNRVRAAVPTIKYLMGHGAKVILSSHLGRPKGVTPKYSLKPLVPRLSELLGVEVKMANDCFGEE 389
            +++ +  GK+ FVRVD NVPLDDN  ITDD R+RAA+PT++ ++  G   I+ SHLGRPKGV P++SL+ ++P +SELLGV+V  A+DC G +
Sbjct:    3 IEQFNFAGKKAFVRVDFNVPLDDNFKITDDTRIRAALPTLRKIIADGGMTIIGSHLGRPKGVAPEFSLRHILPHVSELLGVDVLFADDCIGAD 95

HSP 2 Score: 29.261 bits (64), Expect = 3.220e-28
Identity = 13/21 (61.90%), Postives = 17/21 (80.95%), Query Frame = 1 
Query:  406 AEIPEGGVLLLENVRFHREEE 468
            A++  G VLLLEN+RF+ EEE
Sbjct:  101 AKLKAGQVLLLENLRFYAEEE 121

HSP 3 Score: 22.7126 bits (47), Expect = 3.220e-28
Identity = 13/32 (40.62%), Postives = 17/32 (53.12%), Query Frame = 2 
Query:  467 KRMTPKFAKKLASLADLYGKGRLSDLAH*SHA 562
            K    +F +KLAS+AD Y        AH +HA
Sbjct:  144 KAAQKEFTQKLASMADCYVNDAFG-TAHRAHA 174
[Close]
BLAST of EY710335 vs. ExPASy Swiss-Prot
Match: PGK_SYNP6 (Phosphoglycerate kinase OS=Synechococcus sp. (strain ATCC 27144 / PCC 6301 / SAUG 1402/1) GN=pgk PE=3 SV=1)

HSP 1 Score: 105.531 bits (262), Expect = 3.224e-28
Identity = 58/103 (56.31%), Postives = 68/103 (66.02%), Query Frame = 3 
Query:   90 AKKSVSVLKEADLKGKRVFVRVDLNVPLDDNLNITDDNRVRAAVPTIKYLMGHGAKVILSSHLGRPKG-VTPKYSLKPLVPRLSELLGVEVKMANDCFGEEVE 395
            +K++++ L  ADL+GKRV VRVD NVPLD N  ITDD R+RAA+PTI+YL   GAKVIL SH GRPKG       L P+  RLSELLG  V    D  G   E
Sbjct:    2 SKRTLASLTAADLEGKRVLVRVDFNVPLDGNGKITDDTRIRAALPTIRYLSESGAKVILVSHFGRPKGKPVESMRLTPVAERLSELLGRPVVKTTDAVGAGAE 104

HSP 2 Score: 36.5798 bits (83), Expect = 3.224e-28
Identity = 19/36 (52.78%), Postives = 22/36 (61.11%), Query Frame = 1 
Query:  370 TTALVKKLKKMVAEIPEGGVLLLENVRFHREEEKND 477
            T A+    +  VA    G V+LLENVRFH EEE ND
Sbjct:   96 TDAVGAGAEAQVAATSNGQVVLLENVRFHAEEEAND 131

HSP 3 Score: 25.0238 bits (53), Expect = 3.224e-28
Identity = 14/27 (51.85%), Postives = 17/27 (62.96%), Query Frame = 2 
Query:  482 KFAKKLASLADLYGKGRLSDLAH*SHA 562
            +FAK LASLAD+Y        AH +HA
Sbjct:  133 EFAKALASLADIYVNDAFG-AAHRAHA 158
[Close]
BLAST of EY710335 vs. ExPASy Swiss-Prot
Match: PGK_SYNE7 (Phosphoglycerate kinase OS=Synechococcus elongatus (strain PCC 7942) GN=pgk PE=3 SV=1)

HSP 1 Score: 105.531 bits (262), Expect = 3.224e-28
Identity = 58/103 (56.31%), Postives = 68/103 (66.02%), Query Frame = 3 
Query:   90 AKKSVSVLKEADLKGKRVFVRVDLNVPLDDNLNITDDNRVRAAVPTIKYLMGHGAKVILSSHLGRPKG-VTPKYSLKPLVPRLSELLGVEVKMANDCFGEEVE 395
            +K++++ L  ADL+GKRV VRVD NVPLD N  ITDD R+RAA+PTI+YL   GAKVIL SH GRPKG       L P+  RLSELLG  V    D  G   E
Sbjct:    2 SKRTLASLTAADLEGKRVLVRVDFNVPLDGNGKITDDTRIRAALPTIRYLSESGAKVILVSHFGRPKGKPVESMRLTPVAERLSELLGRPVVKTTDAVGAGAE 104

HSP 2 Score: 36.5798 bits (83), Expect = 3.224e-28
Identity = 19/36 (52.78%), Postives = 22/36 (61.11%), Query Frame = 1 
Query:  370 TTALVKKLKKMVAEIPEGGVLLLENVRFHREEEKND 477
            T A+    +  VA    G V+LLENVRFH EEE ND
Sbjct:   96 TDAVGAGAEAQVAATSNGQVVLLENVRFHAEEEAND 131

HSP 3 Score: 25.0238 bits (53), Expect = 3.224e-28
Identity = 14/27 (51.85%), Postives = 17/27 (62.96%), Query Frame = 2 
Query:  482 KFAKKLASLADLYGKGRLSDLAH*SHA 562
            +FAK LASLAD+Y        AH +HA
Sbjct:  133 EFAKALASLADIYVNDAFG-AAHRAHA 158
The following BLAST results are available for this feature:
BLAST of EY710335 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 500
Match NameE-valueIdentityDescription
PGK_CAMLR1.474e-2852.00Phosphoglycerate kinase OS=Campylobacter lari (str... [more]
PGK_BACCZ1.721e-2856.31Phosphoglycerate kinase OS=Bacillus cereus (strain... [more]
PGK_BACC11.721e-2856.31Phosphoglycerate kinase OS=Bacillus cereus (strain... [more]
PGK_BACSU1.721e-2857.29Phosphoglycerate kinase OS=Bacillus subtilis GN=pg... [more]
PGK_PROMP1.913e-2854.46Phosphoglycerate kinase OS=Prochlorococcus marinus... [more]
PGK_HYDS01.914e-2858.95Phosphoglycerate kinase OS=Hydrogenobaculum sp. (s... [more]
PGK_ANAD22.240e-2855.79Phosphoglycerate kinase OS=Anaeromyxobacter dehalo... [more]
PGK_PORGI3.220e-2853.76Phosphoglycerate kinase OS=Porphyromonas gingivali... [more]
PGK_SYNP63.224e-2856.31Phosphoglycerate kinase OS=Synechococcus sp. (stra... [more]
PGK_SYNE73.224e-2856.31Phosphoglycerate kinase OS=Synechococcus elongatus... [more]

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Properties
Property NameValue
Genbank descriptionCS00-C3-701-097-F04-CT.F Sweet orange fruit, development stadium (2 of 6) Citrus sinensis cDNA, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>EY710335 ID=EY710335; Name=EY710335; organism=Citrus sinensis; type=EST; length=1048bp
CGTTCACCAATCTTCGATCTCATTCCAAAATCTAACCAAAACCCTTTCTC
GATTTCTCTGATCTCGTGTTTGATCGAATCAAAATGGCCGCCAAGAAGAG
TGTAAGCGTTCTAAAAGAAGCTGATTTGAAGGGAAAGAGAGTGTTCGTAC
GAGTGGATCTGAACGTGCCGTTGGATGACAACTTGAATATCACCGATGAC
AACAGAGTCCGTGCTGCTGTTCCCACTATCAAATACCTGATGGGTCATGG
CGCCAAAGTCATTCTCTCCAGTCACTTGGGGCGTCCAAAGGGTGTTACAC
CCAAGTACAGCTTGAAACCTCTTGTGCCAAGGCTTTCTGAACTTCTTGGA
GTTGAGGTTAAAATGGCAAACGACTGCTTTGGTGAAGAAGTTGAAAAAAA
TGGTGGCTGAAATTCCCGAAGGGGGCGTCTTGCTCCTCGAAAATGTGAGG
TTCCATAGGGAGGAAGAAAAGAATGACCCCAAAGTTTGCTAAGAAGCTAG
CTTCCCTGGCAGATCTCTATGGTAAAGGAAGGCTTTCGGATCTGGCCCAT
TAAAGCCATGCTTTCCCCTGAAGGGTGGTGGGCTAATTTCTTTGAAACCC
TTTCTGTGGGGCTGGGTTTCCTTTATGGCAAAAGGGACTAAATTTATCTT
GGTTGGGAGGCTGGTTTCAAATCCCCAAAAAAACCATTTTGGCGGCCCTT
TGTTGGGGGGGTTTAAAAAAGGGGGAAAACTAAAAAAATGGGGGTTTATT
GGATTCCCTTTTTGGGGAAAAAGGGTGAACATTTTTATTTTGCCCGGGGG
GAAAGAATATAATTTTTATCACCTTTATCACAGGGCCCAAAGGGAACAAC
CTTTGGTTGGGTATCATCCCCTTGTGTGAAGGGAAAAAAAAAAAGTTTTT
GTTTACTCCCCCACATTTGGCCCTTTTATGGGAAAAAAGAGGGGACAGAT
TCTTAAAAAGAGGGGGGTGTGGTCTCTATCTATGTATACTCCCCTCTCTA
AGTATGTGGTGTGTTGTTTTTTTTTGTTTTTTGTACGATCAAAAAAAT
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