EY685008

Overview
NameEY685008
Unique NameEY685008
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length909
Libraries
Library NameType
Sweet orange bark, greenhouse plantcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of EY685008 vs. ExPASy Swiss-Prot
Match: PCKA_BREBN (Phosphoenolpyruvate carboxykinase [ATP] OS=Brevibacillus brevis (strain 47 / JCM 6285 / NBRC 100599) GN=pckA PE=3 SV=1)

HSP 1 Score: 169.859 bits (429), Expect = 6.710e-47
Identity = 81/157 (51.59%), Postives = 103/157 (65.61%), Query Frame = 3 
Query:  249 ENTRAAYPIEYIPNAKIPCVGPHPKNVILLACDAFGVLPPVSKLNLAQTMYHFISGYTALVAGTEDGIKEPTATFSACFGAAFIMLHPTKYAAMLAEKMQKHGATGWLVNTGWSGGSYGCGSRIKLPYTRKIIDGIHSGSLLNANYKKTDGFGVGNP 719
            ENTRAAYP+E IP A IP VG  P  ++ L  D+FGVLPP+SKLN  Q MYHF+SGYT+ +AGTE G+  P   FS CFG+ F+ LHP  YA ML +K+ +     +LVNTGW+GG  G G R+KL YTR ++    +G L   +Y   + FGV  P
Sbjct:  315 ENTRAAYPVEAIPGAVIPGVGGQPNVIVFLTADSFGVLPPISKLNKEQAMYHFLSGYTSKMAGTERGVTAPQTEFSTCFGSPFLPLHPVVYAEMLGKKIDERKVQVYLVNTGWTGGPVGVGQRMKLSYTRAMVTAALNGELEKVDYVADEIFGVQVP 471

HSP 2 Score: 37.7354 bits (86), Expect = 6.710e-47
Identity = 17/45 (37.78%), Postives = 23/45 (51.11%), Query Frame = 2 
Query:  701 IWGGKSPLKSKGVPSEILDPKNIWANKQVYKGNLVKLVGFFKRTF 835
            I+G + P     VP+E+L P+N WANK+ Y      L   F   F
Sbjct:  465 IFGVQVPTSCPNVPAEVLQPRNTWANKEDYDKQAADLAARFIENF 509
[Close]
BLAST of EY685008 vs. ExPASy Swiss-Prot
Match: PCKA_RHOPS (Phosphoenolpyruvate carboxykinase [ATP] OS=Rhodopseudomonas palustris (strain BisB5) GN=pckA PE=3 SV=1)

HSP 1 Score: 170.629 bits (431), Expect = 8.744e-47
Identity = 81/157 (51.59%), Postives = 102/157 (64.97%), Query Frame = 3 
Query:  249 ENTRAAYPIEYIPNAKIPCVGPHPKNVILLACDAFGVLPPVSKLNLAQTMYHFISGYTALVAGTEDGIKEPTATFSACFGAAFIMLHPTKYAAMLAEKMQKHGATGWLVNTGWSGGSYGCGSRIKLPYTRKIIDGIHSGSLLNANYKKTDGFGVGNP 719
            ENTR+AYP+E IPNA        PKNV++LA DAFGV+PP++KL  AQ MYHF+SGYTA VAGTE G+ EPT  FS CFG+ F+   P+ Y  ML + +  H    WLVNTGW+GG YG G R+ +  TR ++     GSL NA ++    FG   P
Sbjct:  320 ENTRSAYPLESIPNASPTGRAGQPKNVVMLAADAFGVMPPIAKLTPAQAMYHFLSGYTAKVAGTERGVTEPTPEFSTCFGSPFLPRDPSVYGNMLRDLIHNHNVDCWLVNTGWTGGKYGTGHRMPIKVTRALLTAALDGSLRNAEFRTDPYFGFAVP 476

HSP 2 Score: 36.5798 bits (83), Expect = 8.744e-47
Identity = 17/44 (38.64%), Postives = 24/44 (54.55%), Query Frame = 2 
Query:  704 WGGKSPLKSKGVPSEILDPKNIWANKQVYKGNLVKLVGFFKRTF 835
            +G   P    GVPS+IL+P   WA+K  +      LVG F++ F
Sbjct:  471 FGFAVPTALPGVPSDILEPAKTWADKAEFDKTARALVGMFQKNF 514
[Close]
BLAST of EY685008 vs. ExPASy Swiss-Prot
Match: PCKA_GEOTN (Phosphoenolpyruvate carboxykinase [ATP] OS=Geobacillus thermodenitrificans (strain NG80-2) GN=pckA PE=3 SV=1)

HSP 1 Score: 169.859 bits (429), Expect = 8.745e-47
Identity = 81/159 (50.94%), Postives = 103/159 (64.78%), Query Frame = 3 
Query:  243 IAENTRAAYPIEYIPNAKIPCVGPHPKNVILLACDAFGVLPPVSKLNLAQTMYHFISGYTALVAGTEDGIKEPTATFSACFGAAFIMLHPTKYAAMLAEKMQKHGATGWLVNTGWSGGSYGCGSRIKLPYTRKIIDGIHSGSLLNANYKKTDGFGVGNP 719
            + ENTRAAYP++ I N   P V  HP  ++ L  DAFGVLPP+SKL   Q MYHF+SGYT+ +AGTE G+ EP ATFS CFGA F+     +YA ML +K+ +H    +LVNTGW+GG YG GSR+KL YTR ++     G L N    +   FG+  P
Sbjct:  314 LTENTRAAYPLQAIKNIVDPSVAGHPSTIVFLTADAFGVLPPISKLTREQAMYHFLSGYTSKLAGTERGVTEPEATFSTCFGAPFLPRPAVEYAEMLGQKIAEHNVRVFLVNTGWTGGPYGVGSRMKLAYTRAMVQAAVEGELDNVETVQDPIFGLAIP 472

HSP 2 Score: 37.3502 bits (85), Expect = 8.745e-47
Identity = 17/45 (37.78%), Postives = 25/45 (55.56%), Query Frame = 2 
Query:  701 IWGGKSPLKSKGVPSEILDPKNIWANKQVYKGNLVKLVGFFKRTF 835
            I+G   P    GVP ++L P+N WA+KQ Y+    +L   F+  F
Sbjct:  466 IFGLAIPAHVPGVPDDVLRPQNTWADKQAYEQKAKELAEKFRANF 510
[Close]
BLAST of EY685008 vs. ExPASy Swiss-Prot
Match: PCKA_CAMJR (Phosphoenolpyruvate carboxykinase [ATP] OS=Campylobacter jejuni (strain RM1221) GN=pckA PE=3 SV=1)

HSP 1 Score: 178.718 bits (452), Expect = 8.746e-47
Identity = 86/157 (54.78%), Postives = 108/157 (68.79%), Query Frame = 3 
Query:  249 ENTRAAYPIEYIPNAKIPCVGPHPKNVILLACDAFGVLPPVSKLNLAQTMYHFISGYTALVAGTEDGIKEPTATFSACFGAAFIMLHPTKYAAMLAEKMQKHGATGWLVNTGWSGGSYGCGSRIKLPYTRKIIDGIHSGSLLNANYKKTDGFGVGNP 719
            ENTR +YPIE+I N +      HPKN+I L+ DAFG+LPPVSKL+  Q MY+F+SGYTA VAGTE GI EP ATFSACFG  F+ LHPT YA +L EK++KH    +LVNTGWSGGSY  G R+ +  TR  I+ I  GS+    ++  + F +  P
Sbjct:  311 ENTRVSYPIEHIENHEPSLKAGHPKNIIFLSADAFGILPPVSKLSKEQAMYYFLSGYTAKVAGTERGITEPQATFSACFGEPFMPLHPTVYARLLGEKIEKHEVNVYLVNTGWSGGSYSVGKRMSIKATRACINAILDGSITKCEFENFEVFDLAIP 467

HSP 2 Score: 28.4906 bits (62), Expect = 8.746e-47
Identity = 14/39 (35.90%), Postives = 19/39 (48.72%), Query Frame = 2 
Query:  719 PLKSKGVPSEILDPKNIWANKQVYKGNLVKLVGFFKRTF 835
            P   +GV S +L+P N W +K  Y     KL   F + F
Sbjct:  467 PKALEGVESVLLNPINTWLDKNAYIATRDKLAHMFIQNF 505
[Close]
BLAST of EY685008 vs. ExPASy Swiss-Prot
Match: PCKA_RHIE6 (Phosphoenolpyruvate carboxykinase [ATP] OS=Rhizobium etli (strain CIAT 652) GN=pckA PE=3 SV=1)

HSP 1 Score: 172.17 bits (435), Expect = 1.140e-46
Identity = 78/159 (49.06%), Postives = 103/159 (64.78%), Query Frame = 3 
Query:  243 IAENTRAAYPIEYIPNAKIPCVGPHPKNVILLACDAFGVLPPVSKLNLAQTMYHFISGYTALVAGTEDGIKEPTATFSACFGAAFIMLHPTKYAAMLAEKMQKHGATGWLVNTGWSGGSYGCGSRIKLPYTRKIIDGIHSGSLLNANYKKTDGFGVGNP 719
            + ENTR AYP+ +IPNA       HPK +I+L  DAFGV+PP+++L   Q MYHF+SGYTA VAGTE G+ EP ATFS CFGA F+  HP +Y  +L + + +HG   WLVNTGW+GG+YG G R+ +  TR ++    SG L    ++    FG   P
Sbjct:  317 LTENTRCAYPMHFIPNASETGRAGHPKTIIMLTADAFGVMPPIARLTPDQAMYHFLSGYTAKVAGTEKGVVEPEATFSTCFGAPFMPRHPAEYGNLLKDLISRHGVNCWLVNTGWTGGAYGTGKRMPIKATRALLAAALSGELGQVEFRADPNFGFAVP 475

HSP 2 Score: 34.6538 bits (78), Expect = 1.140e-46
Identity = 17/44 (38.64%), Postives = 22/44 (50.00%), Query Frame = 2 
Query:  704 WGGKSPLKSKGVPSEILDPKNIWANKQVYKGNLVKLVGFFKRTF 835
            +G   P+   GV   ILDP++ WA+K  Y     KLV  F   F
Sbjct:  470 FGFAVPVSVNGVDGGILDPRSTWADKTAYDVQAEKLVSMFIANF 513
[Close]
BLAST of EY685008 vs. ExPASy Swiss-Prot
Match: PCKA_OLICO (Phosphoenolpyruvate carboxykinase [ATP] OS=Oligotropha carboxidovorans (strain ATCC 49405 / DSM 1227 / OM5) GN=pckA PE=3 SV=1)

HSP 1 Score: 166.007 bits (419), Expect = 2.523e-46
Identity = 80/158 (50.63%), Postives = 103/158 (65.19%), Query Frame = 3 
Query:  249 ENTRAAYPIEYIPNAKIPCVGPHPKNVILLACDAFGVLPPVSKLNLAQTMYHFISGYTALVAGTEDGI-KEPTATFSACFGAAFIMLHPTKYAAMLAEKMQKHGATGWLVNTGWSGGSYGCGSRIKLPYTRKIIDGIHSGSLLNANYKKTDGFGVGNP 719
            ENTR+AYP++ IPNA        PKN+++LA DAFGVLPP++KL  +Q MYHF+SGYTA VAGTE G+  EP   FSACFGA F+   PT Y  ML + + KH    WLVNTGW+GG +G G+R+ +  TR ++     GSL N  ++    FG   P
Sbjct:  320 ENTRSAYPLDAIPNASPTGCAGQPKNIVMLAADAFGVLPPIAKLTPSQAMYHFLSGYTAKVAGTERGLGSEPQPEFSACFGAPFLPRDPTVYGEMLRKLIAKHNVDCWLVNTGWTGGKFGIGNRMPIKVTRALLTAALDGSLRNVTFRTDPYFGFAVP 477

HSP 2 Score: 39.6614 bits (91), Expect = 2.523e-46
Identity = 19/44 (43.18%), Postives = 25/44 (56.82%), Query Frame = 2 
Query:  704 WGGKSPLKSKGVPSEILDPKNIWANKQVYKGNLVKLVGFFKRTF 835
            +G   P    GVPS+ILDP N WA+K  +K     LV  F++ F
Sbjct:  472 FGFAVPTALPGVPSDILDPINTWADKADFKKTAQALVSMFRQNF 515
[Close]
BLAST of EY685008 vs. ExPASy Swiss-Prot
Match: PCKA_LEPBP (Phosphoenolpyruvate carboxykinase [ATP] OS=Leptospira biflexa serovar Patoc (strain Patoc 1 / ATCC 23582 / Paris) GN=pckA PE=3 SV=1)

HSP 1 Score: 176.407 bits (446), Expect = 2.523e-46
Identity = 87/157 (55.41%), Postives = 109/157 (69.43%), Query Frame = 3 
Query:  249 ENTRAAYPIEYIPNAKIPCVGPHPKNVILLACDAFGVLPPVSKLNLAQTMYHFISGYTALVAGTEDGIKEPTATFSACFGAAFIMLHPTKYAAMLAEKMQKHGATGWLVNTGWSGGSYGCGSRIKLPYTRKIIDGIHSGSLLNANYKKTDGFGVGNP 719
            ENTR +YPI +I N +      HP  VI L  DA+GVLP VSKL++ Q MYHF+SGYTA VAGTE G+KEP ATFSACFG AF+ LHPT YA +L EKM+KH    +L+NTG  GG YG G R+ LP TR+II+ I +G++  + ++K   F V  P
Sbjct:  317 ENTRVSYPIFHIDNIQPGSKAGHPNTVIFLTYDAYGVLPAVSKLSIEQAMYHFLSGYTAKVAGTERGVKEPQATFSACFGQAFMTLHPTYYAKLLGEKMKKHQVNAYLINTGLVGGKYGVGKRMNLPATRQIINEILNGNIEKSEFEKHPVFQVSFP 473

HSP 2 Score: 29.261 bits (64), Expect = 2.523e-46
Identity = 13/39 (33.33%), Postives = 18/39 (46.15%), Query Frame = 2 
Query:  719 PLKSKGVPSEILDPKNIWANKQVYKGNLVKLVGFFKRTF 835
            P    GV + IL+P+N W NK+ Y      L   F   +
Sbjct:  473 PKSVNGVDAHILNPRNAWENKEDYDKTAADLAKQFVENY 511
[Close]
BLAST of EY685008 vs. ExPASy Swiss-Prot
Match: PCKA_LEPBA (Phosphoenolpyruvate carboxykinase [ATP] OS=Leptospira biflexa serovar Patoc (strain Patoc 1 / Ames) GN=pckA PE=3 SV=1)

HSP 1 Score: 176.407 bits (446), Expect = 2.523e-46
Identity = 87/157 (55.41%), Postives = 109/157 (69.43%), Query Frame = 3 
Query:  249 ENTRAAYPIEYIPNAKIPCVGPHPKNVILLACDAFGVLPPVSKLNLAQTMYHFISGYTALVAGTEDGIKEPTATFSACFGAAFIMLHPTKYAAMLAEKMQKHGATGWLVNTGWSGGSYGCGSRIKLPYTRKIIDGIHSGSLLNANYKKTDGFGVGNP 719
            ENTR +YPI +I N +      HP  VI L  DA+GVLP VSKL++ Q MYHF+SGYTA VAGTE G+KEP ATFSACFG AF+ LHPT YA +L EKM+KH    +L+NTG  GG YG G R+ LP TR+II+ I +G++  + ++K   F V  P
Sbjct:  317 ENTRVSYPIFHIDNIQPGSKAGHPNTVIFLTYDAYGVLPAVSKLSIEQAMYHFLSGYTAKVAGTERGVKEPQATFSACFGQAFMTLHPTYYAKLLGEKMKKHQVNAYLINTGLVGGKYGVGKRMNLPATRQIINEILNGNIEKSEFEKHPVFQVSFP 473

HSP 2 Score: 29.261 bits (64), Expect = 2.523e-46
Identity = 13/39 (33.33%), Postives = 18/39 (46.15%), Query Frame = 2 
Query:  719 PLKSKGVPSEILDPKNIWANKQVYKGNLVKLVGFFKRTF 835
            P    GV + IL+P+N W NK+ Y      L   F   +
Sbjct:  473 PKSVNGVDAHILNPRNAWENKEDYDKTAADLAKQFVENY 511
[Close]
BLAST of EY685008 vs. ExPASy Swiss-Prot
Match: PCKA_BACLD (Phosphoenolpyruvate carboxykinase [ATP] OS=Bacillus licheniformis (strain DSM 13 / ATCC 14580) GN=pckA PE=3 SV=1)

HSP 1 Score: 166.777 bits (421), Expect = 4.285e-46
Identity = 80/157 (50.96%), Postives = 101/157 (64.33%), Query Frame = 3 
Query:  249 ENTRAAYPIEYIPNAKIPCVGPHPKNVILLACDAFGVLPPVSKLNLAQTMYHFISGYTALVAGTEDGIKEPTATFSACFGAAFIMLHPTKYAAMLAEKMQKHGATGWLVNTGWSGGSYGCGSRIKLPYTRKIIDGIHSGSLLNANYKKTDGFGVGNP 719
            ENTRAAYPI+ I N   P +  HP+ ++ L  DAFGVLPP+SKL   Q MYHF+SGYT+ +AGTE GI  P ATFS CFG+ F+ L    YA ML  K+ +HG   +LVNTGW+GG YG G R+ L YTR ++     G L  A  +    FG+ +P
Sbjct:  316 ENTRAAYPIDAIDNIVKPSIAAHPQTIVFLTADAFGVLPPISKLTKEQAMYHFLSGYTSKLAGTERGITTPEATFSTCFGSPFLPLPAHVYAEMLGRKIDEHGVGVFLVNTGWTGGGYGTGERMSLAYTRAMVQAAIEGELDKAEMRTDRIFGLHSP 472

HSP 2 Score: 38.1206 bits (87), Expect = 4.285e-46
Identity = 16/45 (35.56%), Postives = 25/45 (55.56%), Query Frame = 2 
Query:  701 IWGGKSPLKSKGVPSEILDPKNIWANKQVYKGNLVKLVGFFKRTF 835
            I+G  SP+   GVP ++LDP   W ++  Y+   + L   FK+ F
Sbjct:  466 IFGLHSPVHVPGVPDQVLDPAKTWTDENEYEEKAIHLAKAFKQNF 510
[Close]
BLAST of EY685008 vs. ExPASy Swiss-Prot
Match: PCKA_PARDP (Phosphoenolpyruvate carboxykinase [ATP] OS=Paracoccus denitrificans (strain Pd 1222) GN=pckA PE=3 SV=1)

HSP 1 Score: 169.859 bits (429), Expect = 5.584e-46
Identity = 78/161 (48.45%), Postives = 106/161 (65.84%), Query Frame = 3 
Query:  237 HGIAENTRAAYPIEYIPNAKIPCVGPHPKNVILLACDAFGVLPPVSKLNLAQTMYHFISGYTALVAGTEDGIKEPTATFSACFGAAFIMLHPTKYAAMLAEKMQKHGATGWLVNTGWSGGSYGCGSRIKLPYTRKIIDGIHSGSLLNANYKKTDGFGVGNP 719
            + + +N R AYP+E I NA    +G  P+NVI+L CDA+GVLPP+++L  AQ MYHF+SG+T+   GTE G+ EPT TFS CFGA F+   P  Y  +L EK+ K GA  WLVNTGW+GG++G G R+ +  TR ++     GSL +A ++K   FG   P
Sbjct:  317 NSLTDNMRCAYPLEQISNASETSLGGQPRNVIMLTCDAYGVLPPIARLTPAQAMYHFLSGFTSKTPGTEVGVTEPTPTFSTCFGAPFMPRRPEVYGKLLQEKIAKTGAACWLVNTGWTGGAFGTGKRMPIKATRALLTAALDGSLNDAQFRKDPNFGFEVP 477

HSP 2 Score: 34.6538 bits (78), Expect = 5.584e-46
Identity = 15/44 (34.09%), Postives = 22/44 (50.00%), Query Frame = 2 
Query:  704 WGGKSPLKSKGVPSEILDPKNIWANKQVYKGNLVKLVGFFKRTF 835
            +G + P+   GV  ++LDP+  W +   Y     KLVG F   F
Sbjct:  472 FGFEVPVSVPGVEDKLLDPRQTWTDGAAYDAQARKLVGMFSDNF 515
The following BLAST results are available for this feature:
BLAST of EY685008 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 284
Match NameE-valueIdentityDescription
PCKA_BREBN6.710e-4751.59Phosphoenolpyruvate carboxykinase [ATP] OS=Breviba... [more]
PCKA_RHOPS8.744e-4751.59Phosphoenolpyruvate carboxykinase [ATP] OS=Rhodops... [more]
PCKA_GEOTN8.745e-4750.94Phosphoenolpyruvate carboxykinase [ATP] OS=Geobaci... [more]
PCKA_CAMJR8.746e-4754.78Phosphoenolpyruvate carboxykinase [ATP] OS=Campylo... [more]
PCKA_RHIE61.140e-4649.06Phosphoenolpyruvate carboxykinase [ATP] OS=Rhizobi... [more]
PCKA_OLICO2.523e-4650.63Phosphoenolpyruvate carboxykinase [ATP] OS=Oligotr... [more]
PCKA_LEPBP2.523e-4655.41Phosphoenolpyruvate carboxykinase [ATP] OS=Leptosp... [more]
PCKA_LEPBA2.523e-4655.41Phosphoenolpyruvate carboxykinase [ATP] OS=Leptosp... [more]
PCKA_BACLD4.285e-4650.96Phosphoenolpyruvate carboxykinase [ATP] OS=Bacillu... [more]
PCKA_PARDP5.584e-4648.45Phosphoenolpyruvate carboxykinase [ATP] OS=Paracoc... [more]

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Properties
Property NameValue
Genbank descriptionCS00-C2-003-007-E05-CT.F Sweet orange bark, greenhouse plant Citrus sinensis cDNA, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>EY685008 ID=EY685008; Name=EY685008; organism=Citrus sinensis; type=EST; length=909bp
TTTAAGTCAATAGCTTAGCTATTTTGACTTTTTCTATGCCATGTTATAAG
GACTTGAACAAAAAGGGGTCTCCAAATATATAAGACTAAGCGAAAAACTT
AAATACTTAAGTGACACCGAAAAGTCTTTTGATCGTGATCTAAAGATCCA
GGAGAATTTATTTAATCTACAGATAAGCTATACCTACATATGCTCTATGA
TCGTGAAGAAAATTTATTTATCTTGTGGCTGTTTTTCATGGGATTGCAGA
GAACACTCGTGCTGCCTACCCCATTGAGTATATCCCCAATGCAAAGATAC
CATGCGTTGGTCCTCATCCCAAGAATGTCATCCTTTTGGCATGTGATGCT
TTTGGTGTACTCCCACCAGTGAGCAAGCTGAACCTGGCACAAACAATGTA
CCATTTCATCAGTGGCTACACTGCTTTGGTAGCTGGAACAGAGGATGGTA
TTAAGGAGCCAACGGCAACATTTTCAGCTTGCTTTGGTGCAGCATTTATC
ATGCTGCATCCTACCAAGTATGCAGCAATGCTGGCTGAGAAGATGCAGAA
GCATGGTGCTACAGGATGGCTCGTCAACACTGGATGGTCAGGTGGAAGCT
ATGGTTGTGGCAGCCGCATCAAGTTACCGTACACTCGNAAAATCATTGAT
GGGATACACTCTGGCAGCCTGTTGAATGCAAATTACAAGAAAACTGATGG
ATTTGGGGTGGGAAATCCCCACTGAAGTCGAAGGGAGTGCCTTCAGAAAT
CTTGGATCCAAAAAACATTTGGGCAAACAAGCAGGTTTACAAGGGAAACT
TGGTTAAGTTGGTTGGTTTTTTCAAAAGGACTTTTTAATATTTCCCAACT
TAAAAATTGGCAAGGGAAAAAGGTTCCTTGAAGAAATCCTTGGAGCCTGG
CTCCATTTT
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