EY685008

Overview
NameEY685008
Unique NameEY685008
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length909
Libraries
Library NameType
Sweet orange bark, greenhouse plantcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of EY685008 vs. ExPASy Swiss-Prot
Match: PCKA_GEOSM (Phosphoenolpyruvate carboxykinase [ATP] OS=Geobacter sp. (strain M21) GN=pckA PE=3 SV=1)

HSP 1 Score: 179.874 bits (455), Expect = 6.298e-51
Identity = 85/157 (54.14%), Postives = 110/157 (70.06%), Query Frame = 3 
Query:  249 ENTRAAYPIEYIPNAKIPCVGPHPKNVILLACDAFGVLPPVSKLNLAQTMYHFISGYTALVAGTEDGIKEPTATFSACFGAAFIMLHPTKYAAMLAEKMQKHGATGWLVNTGWSGGSYGCGSRIKLPYTRKIIDGIHSGSLLNANYKKTDGFGVGNP 719
            ENTRA+YPI +IPN      G HP +VI+L CDAFGVLPP+++L  AQ MYHF+SGYTA VAGTE G+ EP ATFSACFGA F+ L P+ YA +L +K+  H  + WLVNTGWSGG  G G R+K+ Y+R +++    G+L   ++ K   FG+  P
Sbjct:  320 ENTRASYPITHIPNIVGEGCGGHPNHVIMLTCDAFGVLPPIARLTPAQAMYHFLSGYTAKVAGTEAGVTEPQATFSACFGAPFMALRPSLYADLLGKKIASHKVSCWLVNTGWSGGGPGIGGRMKIAYSRALVNAALDGTLGAGSFVKDSVFGLDIP 476

HSP 2 Score: 41.2022 bits (95), Expect = 6.298e-51
Identity = 17/48 (35.42%), Postives = 28/48 (58.33%), Query Frame = 2 
Query:  701 IWGGKSPLKSKGVPSEILDPKNIWANKQVYKGNLVKLVGFFKRTF*YF 844
            ++G   P    G+P++IL+P+N+W++K  Y     KLV  F+  F  F
Sbjct:  470 VFGLDIPSACPGLPADILNPRNVWSDKSAYDATAHKLVEMFRHNFEQF 517
[Close]
BLAST of EY685008 vs. ExPASy Swiss-Prot
Match: PCKA_RHILO (Phosphoenolpyruvate carboxykinase [ATP] OS=Rhizobium loti GN=pckA PE=3 SV=1)

HSP 1 Score: 180.259 bits (456), Expect = 2.369e-50
Identity = 82/159 (51.57%), Postives = 107/159 (67.30%), Query Frame = 3 
Query:  243 IAENTRAAYPIEYIPNAKIPCVGPHPKNVILLACDAFGVLPPVSKLNLAQTMYHFISGYTALVAGTEDGIKEPTATFSACFGAAFIMLHPTKYAAMLAEKMQKHGATGWLVNTGWSGGSYGCGSRIKLPYTRKIIDGIHSGSLLNANYKKTDGFGVGNP 719
            + ENTR AYP+++IPNA       HPKN+I+L  DAFGV+PP+++L  AQ MYHF+SGYTA VAGTE G+ EP ATFS CFGA F+  HP++Y  +L E +  HGA  WLVNTGW+GG+YG G R+ +  TR ++     GSL    ++    FG   P
Sbjct:  317 LTENTRCAYPLDFIPNASKTGRASHPKNIIMLTADAFGVMPPIARLTPAQAMYHFLSGYTAKVAGTEKGVTEPEATFSTCFGAPFMPRHPSEYGNLLRELIASHGADCWLVNTGWTGGAYGTGKRMPIKATRALLTAALDGSLKTGEFRTDANFGFEVP 475

HSP 2 Score: 38.891 bits (89), Expect = 2.369e-50
Identity = 18/44 (40.91%), Postives = 25/44 (56.82%), Query Frame = 2 
Query:  704 WGGKSPLKSKGVPSEILDPKNIWANKQVYKGNLVKLVGFFKRTF 835
            +G + P+   GV S ILDP++ WA+K  Y     +LVG F   F
Sbjct:  470 FGFEVPVAVPGVDSAILDPRSTWADKPAYDRQAARLVGMFAVNF 513
[Close]
BLAST of EY685008 vs. ExPASy Swiss-Prot
Match: PCKA_STAAW (Phosphoenolpyruvate carboxykinase [ATP] OS=Staphylococcus aureus (strain MW2) GN=pckA PE=3 SV=1)

HSP 1 Score: 181.03 bits (458), Expect = 3.089e-50
Identity = 85/157 (54.14%), Postives = 105/157 (66.88%), Query Frame = 3 
Query:  249 ENTRAAYPIEYIPNAKIPCVGPHPKNVILLACDAFGVLPPVSKLNLAQTMYHFISGYTALVAGTEDGIKEPTATFSACFGAAFIMLHPTKYAAMLAEKMQKHGATGWLVNTGWSGGSYGCGSRIKLPYTRKIIDGIHSGSLLNANYKKTDGFGVGNP 719
            ENTRAAYPI +I N  +P    HP  +I L  DAFGV+PP+SKLN  Q MYHF+SG+T+ +AGTE G+ EP  +FS CFGA F  LHPT YA +L E +  H    +LVNTGW+GG YG G RI L YTR++++   SG L NA Y K   FG+  P
Sbjct:  318 ENTRAAYPINHIDNIVVPSKAAHPNTIIFLTADAFGVIPPISKLNKDQAMYHFLSGFTSKLAGTERGVTEPEPSFSTCFGAPFFPLHPTVYADLLGELIDLHDVDVYLVNTGWTGGKYGVGRRISLHYTRQMVNQAISGKLKNAEYTKDSTFGLSIP 474

HSP 2 Score: 37.7354 bits (86), Expect = 3.089e-50
Identity = 22/62 (35.48%), Postives = 33/62 (53.23%), Query Frame = 2 
Query:  704 WGGKSPLKSKGVPSEILDPKNIWANKQVYKGNLVKLVGFFKRTF*YF-PT*KLAREKGSLKK 886
            +G   P+K + VP  IL+P N W++K+ YK     L+  F++ F  F    +   EKGS  K
Sbjct:  469 FGLSIPVKIEDVPKTILNPINAWSDKEKYKAQAEDLIQRFEKNFEKFGEKVEHIAEKGSFNK 530
[Close]
BLAST of EY685008 vs. ExPASy Swiss-Prot
Match: PCKA_STAAS (Phosphoenolpyruvate carboxykinase [ATP] OS=Staphylococcus aureus (strain MSSA476) GN=pckA PE=3 SV=1)

HSP 1 Score: 181.03 bits (458), Expect = 3.089e-50
Identity = 85/157 (54.14%), Postives = 105/157 (66.88%), Query Frame = 3 
Query:  249 ENTRAAYPIEYIPNAKIPCVGPHPKNVILLACDAFGVLPPVSKLNLAQTMYHFISGYTALVAGTEDGIKEPTATFSACFGAAFIMLHPTKYAAMLAEKMQKHGATGWLVNTGWSGGSYGCGSRIKLPYTRKIIDGIHSGSLLNANYKKTDGFGVGNP 719
            ENTRAAYPI +I N  +P    HP  +I L  DAFGV+PP+SKLN  Q MYHF+SG+T+ +AGTE G+ EP  +FS CFGA F  LHPT YA +L E +  H    +LVNTGW+GG YG G RI L YTR++++   SG L NA Y K   FG+  P
Sbjct:  318 ENTRAAYPINHIDNIVVPSKAAHPNTIIFLTADAFGVIPPISKLNKDQAMYHFLSGFTSKLAGTERGVTEPEPSFSTCFGAPFFPLHPTVYADLLGELIDLHDVDVYLVNTGWTGGKYGVGRRISLHYTRQMVNQAISGKLKNAEYTKDSTFGLSIP 474

HSP 2 Score: 37.7354 bits (86), Expect = 3.089e-50
Identity = 22/62 (35.48%), Postives = 33/62 (53.23%), Query Frame = 2 
Query:  704 WGGKSPLKSKGVPSEILDPKNIWANKQVYKGNLVKLVGFFKRTF*YF-PT*KLAREKGSLKK 886
            +G   P+K + VP  IL+P N W++K+ YK     L+  F++ F  F    +   EKGS  K
Sbjct:  469 FGLSIPVKIEDVPKTILNPINAWSDKEKYKAQAEDLIQRFEKNFEKFGEKVEHIAEKGSFNK 530
[Close]
BLAST of EY685008 vs. ExPASy Swiss-Prot
Match: PCKA2_MOOTA (Phosphoenolpyruvate carboxykinase [ATP] 2 OS=Moorella thermoacetica (strain ATCC 39073) GN=pckA2 PE=3 SV=1)

HSP 1 Score: 180.259 bits (456), Expect = 3.089e-50
Identity = 82/156 (52.56%), Postives = 108/156 (69.23%), Query Frame = 3 
Query:  243 IAENTRAAYPIEYIPNAKIPCVGPHPKNVILLACDAFGVLPPVSKLNLAQTMYHFISGYTALVAGTEDGIKEPTATFSACFGAAFIMLHPTKYAAMLAEKMQKHGATGWLVNTGWSGGSYGCGSRIKLPYTRKIIDGIHSGSLLNANYKKTDGFGV 710
            + ENTRAAYP+++IPNA IP +G HP+ V+ L  DAFGV+PP++KL   Q MYHF+SGYT+ +AGTE GI +P ATFS CFGA F+   P  YA +L E++ KHGA+ +LVNTGW+GG  G G R+ LPYTR ++     G L    +     FG+
Sbjct:  312 LTENTRAAYPVDFIPNAVIPGIGGHPRTVVFLTADAFGVMPPIAKLTREQAMYHFLSGYTSKLAGTERGITDPQATFSTCFGAPFLPRSPRVYADLLGERIAKHGASVYLVNTGWTGGPSGIGRRMSLPYTRAMVRAAIKGELEGVEFVPDPVFGI 467

HSP 2 Score: 38.5058 bits (88), Expect = 3.089e-50
Identity = 16/45 (35.56%), Postives = 25/45 (55.56%), Query Frame = 2 
Query:  701 IWGGKSPLKSKGVPSEILDPKNIWANKQVYKGNLVKLVGFFKRTF 835
            ++G   P    GVP+E+L+P++ W +K+ Y     KL G F   F
Sbjct:  464 VFGILVPTSCPGVPAEVLNPRHTWQDKEKYDAMARKLAGLFAENF 508
[Close]
BLAST of EY685008 vs. ExPASy Swiss-Prot
Match: PCKA_DINSH (Phosphoenolpyruvate carboxykinase [ATP] OS=Dinoroseobacter shibae (strain DFL 12) GN=pckA PE=3 SV=1)

HSP 1 Score: 187.193 bits (474), Expect = 4.026e-50
Identity = 86/159 (54.09%), Postives = 109/159 (68.55%), Query Frame = 3 
Query:  243 IAENTRAAYPIEYIPNAKIPCVGPHPKNVILLACDAFGVLPPVSKLNLAQTMYHFISGYTALVAGTEDGIKEPTATFSACFGAAFIMLHPTKYAAMLAEKMQKHGATGWLVNTGWSGGSYGCGSRIKLPYTRKIIDGIHSGSLLNANYKKTDGFGVGNP 719
            +  N R AYP+ YI NA    +G HPKN+I+L CDAFGVLPP+S+L  AQ MYHF+SG+T+ VAGTE G+ EP  TFS CFGA F+   P  Y  +L EK+ KHGAT WLVNTGW+GG+YG GSR+ +  TR ++     GSL +A ++K   FG   P
Sbjct:  319 LTANMRCAYPLNYISNASETALGGHPKNIIMLTCDAFGVLPPISRLTPAQAMYHFLSGFTSKVAGTERGVTEPEPTFSTCFGAPFMPRRPEVYGKLLQEKIAKHGATCWLVNTGWTGGAYGTGSRMPIKATRALLTAALDGSLADATFRKDANFGFDVP 477

HSP 2 Score: 31.187 bits (69), Expect = 4.026e-50
Identity = 14/44 (31.82%), Postives = 20/44 (45.45%), Query Frame = 2 
Query:  704 WGGKSPLKSKGVPSEILDPKNIWANKQVYKGNLVKLVGFFKRTF 835
            +G   P+   GV + +LDP+  W +   Y     KLV  F   F
Sbjct:  472 FGFDVPVTVHGVDATLLDPRGTWEDSAAYDAQAQKLVEMFAENF 515
[Close]
BLAST of EY685008 vs. ExPASy Swiss-Prot
Match: PCKA_RHOS5 (Phosphoenolpyruvate carboxykinase [ATP] OS=Rhodobacter sphaeroides (strain ATCC 17025 / ATH 2.4.3) GN=pckA PE=3 SV=1)

HSP 1 Score: 182.956 bits (463), Expect = 4.026e-50
Identity = 85/159 (53.46%), Postives = 107/159 (67.30%), Query Frame = 3 
Query:  243 IAENTRAAYPIEYIPNAKIPCVGPHPKNVILLACDAFGVLPPVSKLNLAQTMYHFISGYTALVAGTEDGIKEPTATFSACFGAAFIMLHPTKYAAMLAEKMQKHGATGWLVNTGWSGGSYGCGSRIKLPYTRKIIDGIHSGSLLNANYKKTDGFGVGNP 719
            +  NTR AYP+EYI NA    +G HPKNVI+L CDAFGVLPP+++L  AQ MYHF+SG+T+ VAGTE G+ EP  TFS CFGA F+   P  Y  +L EK+   GAT WLVNTGW+GG+YG G R+ +  TR ++     GSL N  ++K   FG   P
Sbjct:  319 LTANTRCAYPLEYISNASATGMGGHPKNVIMLTCDAFGVLPPIARLTPAQAMYHFLSGFTSKVAGTERGVTEPQPTFSTCFGAPFMPRRPEVYGKLLQEKITSLGATCWLVNTGWTGGAYGTGKRMPIKATRALLTAALDGSLANVTFRKDPNFGFEVP 477

HSP 2 Score: 35.4242 bits (80), Expect = 4.026e-50
Identity = 16/44 (36.36%), Postives = 23/44 (52.27%), Query Frame = 2 
Query:  704 WGGKSPLKSKGVPSEILDPKNIWANKQVYKGNLVKLVGFFKRTF 835
            +G + P +  GV S +LDP++ WA+   Y     KLV  F   F
Sbjct:  472 FGFEVPTELHGVDSSLLDPRSTWADPAAYDAQAKKLVEMFANNF 515
[Close]
BLAST of EY685008 vs. ExPASy Swiss-Prot
Match: PCKA_NITSB (Phosphoenolpyruvate carboxykinase [ATP] OS=Nitratiruptor sp. (strain SB155-2) GN=pckA PE=3 SV=1)

HSP 1 Score: 188.348 bits (477), Expect = 5.248e-50
Identity = 90/147 (61.22%), Postives = 107/147 (72.79%), Query Frame = 3 
Query:  249 ENTRAAYPIEYIPNAKIPCVGPHPKNVILLACDAFGVLPPVSKLNLAQTMYHFISGYTALVAGTEDGIKEPTATFSACFGAAFIMLHPTKYAAMLAEKMQKHGATGWLVNTGWSGGSYGCGSRIKLPYTRKIIDGIHSGSLLNANYK 689
            ENTR +YPI +I   K    GPHPKN+I L+ DAFGVLPPVSKL   Q MY+F+SGYTA VAGTE GI EP ATFSACFG AF+ LHPT YA +L EK+ KHG   +LVNTGW+GG YG G R+ LP TR  I+ I +GS+  + Y+
Sbjct:  313 ENTRVSYPIYHICKHKEDLQGPHPKNIIFLSADAFGVLPPVSKLTKEQAMYYFLSGYTAKVAGTERGITEPVATFSACFGEAFLPLHPTVYAKLLGEKIDKHGVNVYLVNTGWTGGPYGIGQRMSLPATRSCINAILNGSITQSEYE 459

HSP 2 Score: 29.6462 bits (65), Expect = 5.248e-50
Identity = 14/39 (35.90%), Postives = 19/39 (48.72%), Query Frame = 2 
Query:  719 PLKSKGVPSEILDPKNIWANKQVYKGNLVKLVGFFKRTF 835
            P   +GV   IL+P+N W +K+ Y   L  L   F   F
Sbjct:  469 PTYVEGVDPAILNPRNTWEDKEAYDRQLEHLAKLFIDNF 507
[Close]
BLAST of EY685008 vs. ExPASy Swiss-Prot
Match: PCKA_BRASB (Phosphoenolpyruvate carboxykinase [ATP] OS=Bradyrhizobium sp. (strain BTAi1 / ATCC BAA-1182) GN=pckA PE=3 SV=1)

HSP 1 Score: 176.407 bits (446), Expect = 6.839e-50
Identity = 84/158 (53.16%), Postives = 107/158 (67.72%), Query Frame = 3 
Query:  249 ENTRAAYPIEYIPNAKIPCVGPHPKNVILLACDAFGVLPPVSKLNLAQTMYHFISGYTALVAGTEDGI-KEPTATFSACFGAAFIMLHPTKYAAMLAEKMQKHGATGWLVNTGWSGGSYGCGSRIKLPYTRKIIDGIHSGSLLNANYKKTDGFGVGNP 719
            ENTR+AYP++YIPNA      PHPKNV++LA DAFGVLPP++KL+ AQ MYHF+SGYTA VAGTE G+  EP   FS CFG+ F+ L P+ Y  ML + + +H    WLVNTGW+GG YG GSR+ +  TR ++     GSL N  ++    FG   P
Sbjct:  319 ENTRSAYPLDYIPNASRTGRAPHPKNVVMLAADAFGVLPPIAKLSPAQAMYHFLSGYTAKVAGTERGLGNEPQPEFSTCFGSPFLPLDPSVYGNMLRQLIAEHNVDCWLVNTGWTGGKYGTGSRMPIKVTRALLTAALDGSLRNVEFRTDKYFGFAVP 476

HSP 2 Score: 41.2022 bits (95), Expect = 6.839e-50
Identity = 20/44 (45.45%), Postives = 25/44 (56.82%), Query Frame = 2 
Query:  704 WGGKSPLKSKGVPSEILDPKNIWANKQVYKGNLVKLVGFFKRTF 835
            +G   P    GVPSEILDP N WA+K  +      LVG F++ F
Sbjct:  471 FGFAVPTALPGVPSEILDPVNTWADKAEFDKTARALVGMFQKNF 514
[Close]
BLAST of EY685008 vs. ExPASy Swiss-Prot
Match: PCKA_SILPO (Phosphoenolpyruvate carboxykinase [ATP] OS=Silicibacter pomeroyi GN=pckA PE=3 SV=1)

HSP 1 Score: 189.504 bits (480), Expect = 6.839e-50
Identity = 85/159 (53.46%), Postives = 112/159 (70.44%), Query Frame = 3 
Query:  243 IAENTRAAYPIEYIPNAKIPCVGPHPKNVILLACDAFGVLPPVSKLNLAQTMYHFISGYTALVAGTEDGIKEPTATFSACFGAAFIMLHPTKYAAMLAEKMQKHGATGWLVNTGWSGGSYGCGSRIKLPYTRKIIDGIHSGSLLNANYKKTDGFGVGNP 719
            +  N R AYP+EYI NA +  +G HPKN+I+L CDAFGVLPP+++L  AQ MYHF+SG+T+ VAGTE G+ EP  TFS CFGA F+   P  Y  +L +K+ +HGAT WLVNTGW+GG+YG GSR+ +  TR ++    +GSL NA ++K   FG   P
Sbjct:  319 LTANMRCAYPLEYISNASVSALGGHPKNIIMLTCDAFGVLPPIARLTPAQAMYHFLSGFTSKVAGTERGVTEPQPTFSTCFGAPFMPRRPEVYGNLLRDKIARHGATCWLVNTGWTGGAYGVGSRMPIKATRALLTAALNGSLNNAEFRKDGNFGFDVP 477

HSP 2 Score: 28.1054 bits (61), Expect = 6.839e-50
Identity = 13/44 (29.55%), Postives = 20/44 (45.45%), Query Frame = 2 
Query:  704 WGGKSPLKSKGVPSEILDPKNIWANKQVYKGNLVKLVGFFKRTF 835
            +G   P+   GV   +LDP+  W ++  +     KLV  F   F
Sbjct:  472 FGFDVPVSVPGVAEVLLDPRRTWDDQGAFDKQAAKLVQMFADNF 515
The following BLAST results are available for this feature:
BLAST of EY685008 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 284
Match NameE-valueIdentityDescription
PCKA_GEOSM6.298e-5154.14Phosphoenolpyruvate carboxykinase [ATP] OS=Geobact... [more]
PCKA_RHILO2.369e-5051.57Phosphoenolpyruvate carboxykinase [ATP] OS=Rhizobi... [more]
PCKA_STAAW3.089e-5054.14Phosphoenolpyruvate carboxykinase [ATP] OS=Staphyl... [more]
PCKA_STAAS3.089e-5054.14Phosphoenolpyruvate carboxykinase [ATP] OS=Staphyl... [more]
PCKA2_MOOTA3.089e-5052.56Phosphoenolpyruvate carboxykinase [ATP] 2 OS=Moore... [more]
PCKA_DINSH4.026e-5054.09Phosphoenolpyruvate carboxykinase [ATP] OS=Dinoros... [more]
PCKA_RHOS54.026e-5053.46Phosphoenolpyruvate carboxykinase [ATP] OS=Rhodoba... [more]
PCKA_NITSB5.248e-5061.22Phosphoenolpyruvate carboxykinase [ATP] OS=Nitrati... [more]
PCKA_BRASB6.839e-5053.16Phosphoenolpyruvate carboxykinase [ATP] OS=Bradyrh... [more]
PCKA_SILPO6.839e-5053.46Phosphoenolpyruvate carboxykinase [ATP] OS=Silicib... [more]

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Properties
Property NameValue
Genbank descriptionCS00-C2-003-007-E05-CT.F Sweet orange bark, greenhouse plant Citrus sinensis cDNA, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>EY685008 ID=EY685008; Name=EY685008; organism=Citrus sinensis; type=EST; length=909bp
TTTAAGTCAATAGCTTAGCTATTTTGACTTTTTCTATGCCATGTTATAAG
GACTTGAACAAAAAGGGGTCTCCAAATATATAAGACTAAGCGAAAAACTT
AAATACTTAAGTGACACCGAAAAGTCTTTTGATCGTGATCTAAAGATCCA
GGAGAATTTATTTAATCTACAGATAAGCTATACCTACATATGCTCTATGA
TCGTGAAGAAAATTTATTTATCTTGTGGCTGTTTTTCATGGGATTGCAGA
GAACACTCGTGCTGCCTACCCCATTGAGTATATCCCCAATGCAAAGATAC
CATGCGTTGGTCCTCATCCCAAGAATGTCATCCTTTTGGCATGTGATGCT
TTTGGTGTACTCCCACCAGTGAGCAAGCTGAACCTGGCACAAACAATGTA
CCATTTCATCAGTGGCTACACTGCTTTGGTAGCTGGAACAGAGGATGGTA
TTAAGGAGCCAACGGCAACATTTTCAGCTTGCTTTGGTGCAGCATTTATC
ATGCTGCATCCTACCAAGTATGCAGCAATGCTGGCTGAGAAGATGCAGAA
GCATGGTGCTACAGGATGGCTCGTCAACACTGGATGGTCAGGTGGAAGCT
ATGGTTGTGGCAGCCGCATCAAGTTACCGTACACTCGNAAAATCATTGAT
GGGATACACTCTGGCAGCCTGTTGAATGCAAATTACAAGAAAACTGATGG
ATTTGGGGTGGGAAATCCCCACTGAAGTCGAAGGGAGTGCCTTCAGAAAT
CTTGGATCCAAAAAACATTTGGGCAAACAAGCAGGTTTACAAGGGAAACT
TGGTTAAGTTGGTTGGTTTTTTCAAAAGGACTTTTTAATATTTCCCAACT
TAAAAATTGGCAAGGGAAAAAGGTTCCTTGAAGAAATCCTTGGAGCCTGG
CTCCATTTT
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