EY685008

Overview
NameEY685008
Unique NameEY685008
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length909
Libraries
Library NameType
Sweet orange bark, greenhouse plantcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of EY685008 vs. ExPASy Swiss-Prot
Match: PCKA_SHEAM (Phosphoenolpyruvate carboxykinase [ATP] OS=Shewanella amazonensis (strain ATCC BAA-1098 / SB2B) GN=pckA PE=3 SV=1)

HSP 1 Score: 139.043 bits (349), Expect = 2.719e-35
Identity = 71/144 (49.31%), Postives = 90/144 (62.50%), Query Frame = 3 
Query:  243 IAENTRAAYPIEYIPNAKIPCVGPHPKNVILLACDAFGVLPPVSKLNLAQTMYHFISGYTALVAGTEDGIKEP-TATFSACFGAAFIMLHPTKYAAMLAEKMQKHGATGWLVNTGWSGGSYGCGSRIKLPYTRKIIDGIHSGSL 671
            + ENTRAAYP+E+I   +    G  P+ V+ L CD  GVLPPVSKL   Q  YHF+SGYTA V  TE G      +TFS CFGA F       YA +L +++++ G+  +LVNTGW+GG YG G R  +P TR I+D I SG L
Sbjct:  300 LTENTRAAYPLEHIAQRQEENRGGEPRAVVFLTCDVSGVLPPVSKLTKEQAAYHFLSGYTAKVGSTEMGSTAAIQSTFSTCFGAPFFPRPAGVYAELLMKRIEEFGSQVYLVNTGWTGGPYGVGKRFDIPTTRAIVDAIVSGEL 443

HSP 2 Score: 29.6462 bits (65), Expect = 2.719e-35
Identity = 14/39 (35.90%), Postives = 21/39 (53.85%), Query Frame = 2 
Query:  719 PLKSKGVPSEILDPKNIWANKQVYKGNLVKLVGFFKRTF 835
            P+   GV + +L+P N WA+K  Y+    KL   F+  F
Sbjct:  459 PVAVPGVETALLNPVNTWADKAKYQEYAQKLAEEFQANF 497
[Close]
BLAST of EY685008 vs. ExPASy Swiss-Prot
Match: PCKA_SHEDO (Phosphoenolpyruvate carboxykinase [ATP] OS=Shewanella denitrificans (strain OS217 / ATCC BAA-1090 / DSM 15013) GN=pckA PE=3 SV=1)

HSP 1 Score: 137.117 bits (344), Expect = 3.542e-35
Identity = 71/147 (48.30%), Postives = 90/147 (61.22%), Query Frame = 3 
Query:  243 IAENTRAAYPIEYIPNAKIPCVGPHPKNVILLACDAFGVLPPVSKLNLAQTMYHFISGYTALVAGTEDGIKEP-TATFSACFGAAFIMLHPTKYAAMLAEKMQKHGATGWLVNTGWSGGSYGCGSRIKLPYTRKIIDGIHSGSLLNA 680
            + EN+RAAYP+E+I   K+   G  P  V+ L CD  GVLPPVS L   Q  YHF+SGYTA V  TE G      +TFS CFGA F       YA +L E+++   +  +LVNTGW+GG +G G R  +P TR I+D I SGSL +A
Sbjct:  300 LTENSRAAYPLEHIAQRKVENRGNEPHAVVFLTCDVSGVLPPVSILTKEQAAYHFLSGYTAKVGSTEMGSTSAIQSTFSTCFGAPFFPRPAGVYAELLIERIESFNSQVYLVNTGWTGGPHGVGKRFDIPTTRAIVDAIVSGSLKDA 446

HSP 2 Score: 31.187 bits (69), Expect = 3.542e-35
Identity = 15/39 (38.46%), Postives = 21/39 (53.85%), Query Frame = 2 
Query:  719 PLKSKGVPSEILDPKNIWANKQVYKGNLVKLVGFFKRTF 835
            PL  +GV S +L+P N WA+K +Y     +L   F   F
Sbjct:  459 PLAIEGVDSTLLNPINTWADKDMYAKYAKQLAQEFNSNF 497
[Close]
BLAST of EY685008 vs. ExPASy Swiss-Prot
Match: PCKA_SHEPA (Phosphoenolpyruvate carboxykinase [ATP] OS=Shewanella pealeana (strain ATCC 700345 / ANG-SQ1) GN=pckA PE=3 SV=1)

HSP 1 Score: 140.198 bits (352), Expect = 4.614e-35
Identity = 71/144 (49.31%), Postives = 89/144 (61.81%), Query Frame = 3 
Query:  243 IAENTRAAYPIEYIPNAKIPCVGPHPKNVILLACDAFGVLPPVSKLNLAQTMYHFISGYTALVAGTEDGIKEP-TATFSACFGAAFIMLHPTKYAAMLAEKMQKHGATGWLVNTGWSGGSYGCGSRIKLPYTRKIIDGIHSGSL 671
            + ENTRAAYP+E+I   K+   G  P  V+ L CD  GVLPPVSKL   Q  YHF+SGYTA V  TE G      +TFS CFGA F       YA +L ++++  G+  +LVNTGW+GG +G G R  +P TR I+D I SG L
Sbjct:  300 LTENTRAAYPLEHIAQRKLDNCGAEPHAVVFLTCDVSGVLPPVSKLTKEQAAYHFLSGYTAKVGSTEMGSTAAIQSTFSTCFGAPFFPRPAGVYAELLMKRIESFGSQVYLVNTGWTGGPHGIGKRFDIPTTRAIVDAIVSGEL 443

HSP 2 Score: 27.7202 bits (60), Expect = 4.614e-35
Identity = 14/39 (35.90%), Postives = 19/39 (48.72%), Query Frame = 2 
Query:  719 PLKSKGVPSEILDPKNIWANKQVYKGNLVKLVGFFKRTF 835
            P+   GV S++L+P N W +K  Y      L   FK  F
Sbjct:  459 PVAIPGVDSKLLNPINTWEDKVQYAEFAQHLADSFKANF 497
[Close]
BLAST of EY685008 vs. ExPASy Swiss-Prot
Match: PCKA_SHESR (Phosphoenolpyruvate carboxykinase [ATP] OS=Shewanella sp. (strain MR-7) GN=pckA PE=3 SV=1)

HSP 1 Score: 137.887 bits (346), Expect = 4.614e-35
Identity = 73/160 (45.62%), Postives = 92/160 (57.50%), Query Frame = 3 
Query:  243 IAENTRAAYPIEYIPNAKIPCVGPHPKNVILLACDAFGVLPPVSKLNLAQTMYHFISGYTALVAGTEDGIKEP-TATFSACFGAAFIMLHPTKYAAMLAEKMQKHGATGWLVNTGWSGGSYGCGSRIKLPYTRKIIDGIHSGSLLNANYKKTDGFGVGNP 719
            + ENTRAAYP+E+I   K    G  P  V+ L CD  GVLPPVS L+  Q  YHF+SGYTA V  TE G      +TFS CFGA F       YA +L ++++  G+  +LVNTGW+GG +G G R  +P TR I+D I SG L N      D   +  P
Sbjct:  300 LTENTRAAYPLEHIAQRKEDNRGAEPHAVVFLTCDVSGVLPPVSILSKEQAAYHFLSGYTAKVGSTEIGSTSAIQSTFSTCFGAPFFPRPAGVYAELLMKRIESFGSQVYLVNTGWTGGPHGIGKRFDIPTTRAIVDAIVSGELKNVETVHLDTLNLAVP 459

HSP 2 Score: 30.0314 bits (66), Expect = 4.614e-35
Identity = 13/39 (33.33%), Postives = 21/39 (53.85%), Query Frame = 2 
Query:  719 PLKSKGVPSEILDPKNIWANKQVYKGNLVKLVGFFKRTF 835
            P+   GV + +L+P N W++K +Y     KL   F + F
Sbjct:  459 PVAVPGVDTNLLNPVNTWSDKALYAEYAQKLAEAFTKNF 497
[Close]
BLAST of EY685008 vs. ExPASy Swiss-Prot
Match: PCKA_SHEPW (Phosphoenolpyruvate carboxykinase [ATP] OS=Shewanella piezotolerans (strain WP3 / JCM 13877) GN=pckA PE=3 SV=1)

HSP 1 Score: 138.272 bits (347), Expect = 6.009e-35
Identity = 71/144 (49.31%), Postives = 88/144 (61.11%), Query Frame = 3 
Query:  243 IAENTRAAYPIEYIPNAKIPCVGPHPKNVILLACDAFGVLPPVSKLNLAQTMYHFISGYTALVAGTEDGIKEP-TATFSACFGAAFIMLHPTKYAAMLAEKMQKHGATGWLVNTGWSGGSYGCGSRIKLPYTRKIIDGIHSGSL 671
            + ENTRAAYP+E+I   K    G  P  V+ L CD  GVLPPVSKL   Q  YHF+SGYTA V  TE G      +TFS CFGA F       YA +L ++++  G+  +LVNTGW+GG +G G R  +P TR I+D I SG L
Sbjct:  300 LTENTRAAYPLEHIAQRKEENCGAEPHAVVFLTCDVSGVLPPVSKLTKEQAAYHFLSGYTAKVGSTEMGSTAAIQSTFSTCFGAPFFPRPAGVYAELLMKRIESFGSQVYLVNTGWTGGPHGIGKRFDIPTTRAIVDAIVSGEL 443

HSP 2 Score: 29.261 bits (64), Expect = 6.009e-35
Identity = 14/39 (35.90%), Postives = 20/39 (51.28%), Query Frame = 2 
Query:  719 PLKSKGVPSEILDPKNIWANKQVYKGNLVKLVGFFKRTF 835
            P+   GV S +L+P N W +KQ Y      L   F++ F
Sbjct:  459 PVAIPGVDSNLLNPINTWEDKQQYAEFAQHLAESFQKNF 497
[Close]
BLAST of EY685008 vs. ExPASy Swiss-Prot
Match: PCKA_PSEPW (Phosphoenolpyruvate carboxykinase [ATP] OS=Pseudomonas putida (strain W619) GN=pckA PE=3 SV=1)

HSP 1 Score: 137.117 bits (344), Expect = 6.009e-35
Identity = 72/156 (46.15%), Postives = 94/156 (60.26%), Query Frame = 3 
Query:  243 IAENTRAAYPIEYIPNAKIPCVGPHPKNVILLACDAFGVLPPVSKLNLAQTMYHFISGYTALVAGTE----DGIKEPTATFSACFGAAFIMLHPTKYAAMLAEKMQKHGATGWLVNTGWSGGSYGCGSRIKLPYTRKIIDGIHSGSLLNANYKKTD 698
            + +N+RAAYP+E++       +G  P  VI L CD  GVLPPVS LN  Q  YHF+SGYTALV  TE     GIK   +TFS CFGA F      +YA +L +++   G+  +LVNTGW+GG YG G R  +P TR +I  I SG+L+    +  D
Sbjct:  300 LTQNSRAAYPLEHVAKRAEANLGGEPNAVIFLTCDLTGVLPPVSILNNEQAAYHFLSGYTALVGSTEMGSGGGIK---STFSTCFGAPFFPRPAGEYAELLIKRINAFGSKVYLVNTGWTGGGYGVGKRFSIPTTRGVIAAIQSGALVGTETEHLD 452

HSP 2 Score: 30.4166 bits (67), Expect = 6.009e-35
Identity = 14/39 (35.90%), Postives = 19/39 (48.72%), Query Frame = 2 
Query:  719 PLKSKGVPSEILDPKNIWANKQVYKGNLVKLVGFFKRTF 835
            P    GV +E+L+P+N WA+K  Y      L   F   F
Sbjct:  459 PKAVPGVETELLNPRNTWADKAAYDEAAKGLAKLFTENF 497
[Close]
BLAST of EY685008 vs. ExPASy Swiss-Prot
Match: PCKA_PSEE4 (Phosphoenolpyruvate carboxykinase [ATP] OS=Pseudomonas entomophila (strain L48) GN=pckA PE=3 SV=1)

HSP 1 Score: 136.732 bits (343), Expect = 6.009e-35
Identity = 72/156 (46.15%), Postives = 95/156 (60.90%), Query Frame = 3 
Query:  243 IAENTRAAYPIEYIPNAKIPCVGPHPKNVILLACDAFGVLPPVSKLNLAQTMYHFISGYTALVAGTE----DGIKEPTATFSACFGAAFIMLHPTKYAAMLAEKMQKHGATGWLVNTGWSGGSYGCGSRIKLPYTRKIIDGIHSGSLLNANYKKTD 698
            + +N+RAAYP+E++       +G  P  VI L CD  GVLPPVS LN  Q  YHF+SGYTALV  TE     GIK   +TFS CFGA F      +YA +L ++++   +  +LVNTGW+GG YG G R  +P TR +I  I SG+L+ A  +  D
Sbjct:  300 LTQNSRAAYPLEHVAKRSEKNLGGEPNAVIFLTCDLTGVLPPVSILNNEQAAYHFLSGYTALVGSTEMGSGGGIK---STFSTCFGAPFFPRPAGEYAELLIKRIKGFDSKVYLVNTGWTGGGYGVGKRFSIPTTRGVIAAIQSGALIGAETEHLD 452

HSP 2 Score: 30.8018 bits (68), Expect = 6.009e-35
Identity = 16/38 (42.11%), Postives = 20/38 (52.63%), Query Frame = 2 
Query:  731 KGVPS---EILDPKNIWANKQVYKGNLVKLVGFFKRTF 835
            K VPS   E+L+P+N WA+K  Y      L G F   F
Sbjct:  460 KAVPSVETELLNPRNTWADKAAYDTAAKGLAGLFIENF 497
[Close]
BLAST of EY685008 vs. ExPASy Swiss-Prot
Match: PCKA_PSEMY (Phosphoenolpyruvate carboxykinase [ATP] OS=Pseudomonas mendocina (strain ymp) GN=pckA PE=3 SV=1)

HSP 1 Score: 139.043 bits (349), Expect = 7.827e-35
Identity = 73/150 (48.67%), Postives = 92/150 (61.33%), Query Frame = 3 
Query:  243 IAENTRAAYPIEYIPNAKIPCVGPHPKNVILLACDAFGVLPPVSKLNLAQTMYHFISGYTALVAGTE----DGIKEPTATFSACFGAAFIMLHPTKYAAMLAEKMQKHGATGWLVNTGWSGGSYGCGSRIKLPYTRKIIDGIHSGSLLNA 680
            + +N+RAAYP+EY+       +G  P  VI L CD  GVLPPVS LN  Q  YHF+SGYTALV  TE     GIK   +TFS CFGA F       YA +L +++   G+  +LVNTGW+GG YG G R  +P TR +I  I SG+L+ A
Sbjct:  300 LTQNSRAAYPLEYVEKRSEKNLGGEPNAVIFLTCDLTGVLPPVSILNNEQAAYHFLSGYTALVGSTEMGSGGGIK---STFSTCFGAPFFPRPAGVYAELLIKRINAFGSKVYLVNTGWTGGGYGVGKRFNIPTTRGVIAAIQSGALIGA 446

HSP 2 Score: 28.1054 bits (61), Expect = 7.827e-35
Identity = 10/24 (41.67%), Postives = 15/24 (62.50%), Query Frame = 2 
Query:  719 PLKSKGVPSEILDPKNIWANKQVY 790
            P    GV + +L+P+N WA+K  Y
Sbjct:  459 PKSVPGVETNLLNPRNTWADKNAY 482
[Close]
BLAST of EY685008 vs. ExPASy Swiss-Prot
Match: PCKA_SHEB9 (Phosphoenolpyruvate carboxykinase [ATP] OS=Shewanella baltica (strain OS195) GN=pckA PE=3 SV=1)

HSP 1 Score: 136.346 bits (342), Expect = 7.827e-35
Identity = 72/160 (45.00%), Postives = 91/160 (56.88%), Query Frame = 3 
Query:  243 IAENTRAAYPIEYIPNAKIPCVGPHPKNVILLACDAFGVLPPVSKLNLAQTMYHFISGYTALVAGTEDGIKEP-TATFSACFGAAFIMLHPTKYAAMLAEKMQKHGATGWLVNTGWSGGSYGCGSRIKLPYTRKIIDGIHSGSLLNANYKKTDGFGVGNP 719
            + ENTRAAYP+E+I   K    G  P  V+ L CD  GVLPPVS L   Q  YHF+SGYTA V  TE G      +TFS CFGA F       YA +L ++++  G+  +LVNTGW+GG +G G R  +P TR I+D I SG L +      D   +  P
Sbjct:  300 LTENTRAAYPLEHIAQRKEDNCGAEPHAVVFLTCDVSGVLPPVSILTKEQAAYHFLSGYTAKVGSTEIGSTSAIQSTFSTCFGAPFFPRPAGVYAELLMKRIESFGSQVYLVNTGWTGGPHGVGKRFDIPTTRAIVDAIVSGELKDVETVHLDTLNLAVP 459

HSP 2 Score: 30.8018 bits (68), Expect = 7.827e-35
Identity = 14/39 (35.90%), Postives = 20/39 (51.28%), Query Frame = 2 
Query:  719 PLKSKGVPSEILDPKNIWANKQVYKGNLVKLVGFFKRTF 835
            P+   GV S +L+P N W +K +Y     KL   F + F
Sbjct:  459 PVAVSGVDSNLLNPINTWGDKALYAEYAQKLAEAFTKNF 497
[Close]
BLAST of EY685008 vs. ExPASy Swiss-Prot
Match: PCKA_SHEB8 (Phosphoenolpyruvate carboxykinase [ATP] OS=Shewanella baltica (strain OS185) GN=pckA PE=3 SV=1)

HSP 1 Score: 136.346 bits (342), Expect = 7.827e-35
Identity = 72/160 (45.00%), Postives = 91/160 (56.88%), Query Frame = 3 
Query:  243 IAENTRAAYPIEYIPNAKIPCVGPHPKNVILLACDAFGVLPPVSKLNLAQTMYHFISGYTALVAGTEDGIKEP-TATFSACFGAAFIMLHPTKYAAMLAEKMQKHGATGWLVNTGWSGGSYGCGSRIKLPYTRKIIDGIHSGSLLNANYKKTDGFGVGNP 719
            + ENTRAAYP+E+I   K    G  P  V+ L CD  GVLPPVS L   Q  YHF+SGYTA V  TE G      +TFS CFGA F       YA +L ++++  G+  +LVNTGW+GG +G G R  +P TR I+D I SG L +      D   +  P
Sbjct:  300 LTENTRAAYPLEHIAQRKEDNCGAEPHAVVFLTCDVSGVLPPVSILTKEQAAYHFLSGYTAKVGSTEIGSTSAIQSTFSTCFGAPFFPRPAGVYAELLMKRIESFGSQVYLVNTGWTGGPHGVGKRFDIPTTRAIVDAIVSGELKDVETVHLDTLNLAVP 459

HSP 2 Score: 30.8018 bits (68), Expect = 7.827e-35
Identity = 14/39 (35.90%), Postives = 21/39 (53.85%), Query Frame = 2 
Query:  719 PLKSKGVPSEILDPKNIWANKQVYKGNLVKLVGFFKRTF 835
            P+   GV S +L+P N W++K +Y     KL   F + F
Sbjct:  459 PVAVTGVDSNLLNPINTWSDKALYAEYAQKLAEAFTKNF 497
The following BLAST results are available for this feature:
BLAST of EY685008 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 284
Match NameE-valueIdentityDescription
PCKA_SHEAM2.719e-3549.31Phosphoenolpyruvate carboxykinase [ATP] OS=Shewane... [more]
PCKA_SHEDO3.542e-3548.30Phosphoenolpyruvate carboxykinase [ATP] OS=Shewane... [more]
PCKA_SHEPA4.614e-3549.31Phosphoenolpyruvate carboxykinase [ATP] OS=Shewane... [more]
PCKA_SHESR4.614e-3545.63Phosphoenolpyruvate carboxykinase [ATP] OS=Shewane... [more]
PCKA_SHEPW6.009e-3549.31Phosphoenolpyruvate carboxykinase [ATP] OS=Shewane... [more]
PCKA_PSEPW6.009e-3546.15Phosphoenolpyruvate carboxykinase [ATP] OS=Pseudom... [more]
PCKA_PSEE46.009e-3546.15Phosphoenolpyruvate carboxykinase [ATP] OS=Pseudom... [more]
PCKA_PSEMY7.827e-3548.67Phosphoenolpyruvate carboxykinase [ATP] OS=Pseudom... [more]
PCKA_SHEB97.827e-3545.00Phosphoenolpyruvate carboxykinase [ATP] OS=Shewane... [more]
PCKA_SHEB87.827e-3545.00Phosphoenolpyruvate carboxykinase [ATP] OS=Shewane... [more]

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Properties
Property NameValue
Genbank descriptionCS00-C2-003-007-E05-CT.F Sweet orange bark, greenhouse plant Citrus sinensis cDNA, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>EY685008 ID=EY685008; Name=EY685008; organism=Citrus sinensis; type=EST; length=909bp
TTTAAGTCAATAGCTTAGCTATTTTGACTTTTTCTATGCCATGTTATAAG
GACTTGAACAAAAAGGGGTCTCCAAATATATAAGACTAAGCGAAAAACTT
AAATACTTAAGTGACACCGAAAAGTCTTTTGATCGTGATCTAAAGATCCA
GGAGAATTTATTTAATCTACAGATAAGCTATACCTACATATGCTCTATGA
TCGTGAAGAAAATTTATTTATCTTGTGGCTGTTTTTCATGGGATTGCAGA
GAACACTCGTGCTGCCTACCCCATTGAGTATATCCCCAATGCAAAGATAC
CATGCGTTGGTCCTCATCCCAAGAATGTCATCCTTTTGGCATGTGATGCT
TTTGGTGTACTCCCACCAGTGAGCAAGCTGAACCTGGCACAAACAATGTA
CCATTTCATCAGTGGCTACACTGCTTTGGTAGCTGGAACAGAGGATGGTA
TTAAGGAGCCAACGGCAACATTTTCAGCTTGCTTTGGTGCAGCATTTATC
ATGCTGCATCCTACCAAGTATGCAGCAATGCTGGCTGAGAAGATGCAGAA
GCATGGTGCTACAGGATGGCTCGTCAACACTGGATGGTCAGGTGGAAGCT
ATGGTTGTGGCAGCCGCATCAAGTTACCGTACACTCGNAAAATCATTGAT
GGGATACACTCTGGCAGCCTGTTGAATGCAAATTACAAGAAAACTGATGG
ATTTGGGGTGGGAAATCCCCACTGAAGTCGAAGGGAGTGCCTTCAGAAAT
CTTGGATCCAAAAAACATTTGGGCAAACAAGCAGGTTTACAAGGGAAACT
TGGTTAAGTTGGTTGGTTTTTTCAAAAGGACTTTTTAATATTTCCCAACT
TAAAAATTGGCAAGGGAAAAAGGTTCCTTGAAGAAATCCTTGGAGCCTGG
CTCCATTTT
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