EY685008 | Citrus Genome Database

Overview


Name	EY685008
Unique Name	EY685008
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	909

Libraries


Library Name	Type
Sweet orange bark, greenhouse plant	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Homology

BLAST of EY685008 vs. ExPASy Swiss-Prot
Match: PCKA_STAA9 (Phosphoenolpyruvate carboxykinase [ATP] OS=Staphylococcus aureus (strain JH9) GN=pckA PE=3 SV=1)

HSP 1 Score: 181.03 bits (458), Expect = 8.915e-50
Identity = 85/157 (54.14%), Postives = 105/157 (66.88%), Query Frame = 3

Query:  249 ENTRAAYPIEYIPNAKIPCVGPHPKNVILLACDAFGVLPPVSKLNLAQTMYHFISGYTALVAGTEDGIKEPTATFSACFGAAFIMLHPTKYAAMLAEKMQKHGATGWLVNTGWSGGSYGCGSRIKLPYTRKIIDGIHSGSLLNANYKKTDGFGVGNP 719
            ENTRAAYPI +I N  +P    HP  +I L  DAFGV+PP+SKLN  Q MYHF+SG+T+ +AGTE G+ EP  +FS CFGA F  LHPT YA +L E +  H    +LVNTGW+GG YG G RI L YTR++++   SG L NA Y K   FG+  P
Sbjct:  318 ENTRAAYPINHIDNIVVPSKAAHPNTIIFLTADAFGVIPPISKLNKDQAMYHFLSGFTSKLAGTERGVTEPEPSFSTCFGAPFFPLHPTVYADLLGELIDLHDVDVYLVNTGWTGGKYGVGRRISLHYTRQMVNQAISGKLKNAEYTKDSTFGLSIP 474

HSP 2 Score: 36.1946 bits (82), Expect = 8.915e-50
Identity = 21/62 (33.87%), Postives = 33/62 (53.23%), Query Frame = 2

Query:  704 WGGKSPLKSKGVPSEILDPKNIWANKQVYKGNLVKLVGFFKRTF*YF-PT*KLAREKGSLKK 886
            +G   P++ + VP  IL+P N W++K+ YK     L+  F++ F  F    +   EKGS  K
Sbjct:  469 FGLSIPVEIEDVPKTILNPINAWSDKEKYKAQAEDLIQRFEKNFEKFGEKVEHIAEKGSFNK 530

BLAST of EY685008 vs. ExPASy Swiss-Prot
Match: PCKA_STAA8 (Phosphoenolpyruvate carboxykinase [ATP] OS=Staphylococcus aureus (strain NCTC 8325) GN=pckA PE=3 SV=1)

HSP 1 Score: 181.03 bits (458), Expect = 8.915e-50
Identity = 85/157 (54.14%), Postives = 105/157 (66.88%), Query Frame = 3

Query:  249 ENTRAAYPIEYIPNAKIPCVGPHPKNVILLACDAFGVLPPVSKLNLAQTMYHFISGYTALVAGTEDGIKEPTATFSACFGAAFIMLHPTKYAAMLAEKMQKHGATGWLVNTGWSGGSYGCGSRIKLPYTRKIIDGIHSGSLLNANYKKTDGFGVGNP 719
            ENTRAAYPI +I N  +P    HP  +I L  DAFGV+PP+SKLN  Q MYHF+SG+T+ +AGTE G+ EP  +FS CFGA F  LHPT YA +L E +  H    +LVNTGW+GG YG G RI L YTR++++   SG L NA Y K   FG+  P
Sbjct:  318 ENTRAAYPINHIDNIVVPSKAAHPNTIIFLTADAFGVIPPISKLNKDQAMYHFLSGFTSKLAGTERGVTEPEPSFSTCFGAPFFPLHPTVYADLLGELIDLHDVDVYLVNTGWTGGKYGVGRRISLHYTRQMVNQAISGKLKNAEYTKDSTFGLSIP 474

HSP 2 Score: 36.1946 bits (82), Expect = 8.915e-50
Identity = 21/62 (33.87%), Postives = 33/62 (53.23%), Query Frame = 2

Query:  704 WGGKSPLKSKGVPSEILDPKNIWANKQVYKGNLVKLVGFFKRTF*YF-PT*KLAREKGSLKK 886
            +G   P++ + VP  IL+P N W++K+ YK     L+  F++ F  F    +   EKGS  K
Sbjct:  469 FGLSIPVEIEDVPKTILNPINAWSDKEKYKAQAEDLIQRFEKNFEKFGEKVEHIAEKGSFNK 530

BLAST of EY685008 vs. ExPASy Swiss-Prot
Match: PCKA_STAA3 (Phosphoenolpyruvate carboxykinase [ATP] OS=Staphylococcus aureus (strain USA300) GN=pckA PE=3 SV=1)

HSP 1 Score: 181.03 bits (458), Expect = 8.915e-50
Identity = 85/157 (54.14%), Postives = 105/157 (66.88%), Query Frame = 3

Query:  249 ENTRAAYPIEYIPNAKIPCVGPHPKNVILLACDAFGVLPPVSKLNLAQTMYHFISGYTALVAGTEDGIKEPTATFSACFGAAFIMLHPTKYAAMLAEKMQKHGATGWLVNTGWSGGSYGCGSRIKLPYTRKIIDGIHSGSLLNANYKKTDGFGVGNP 719
            ENTRAAYPI +I N  +P    HP  +I L  DAFGV+PP+SKLN  Q MYHF+SG+T+ +AGTE G+ EP  +FS CFGA F  LHPT YA +L E +  H    +LVNTGW+GG YG G RI L YTR++++   SG L NA Y K   FG+  P
Sbjct:  318 ENTRAAYPINHIDNIVVPSKAAHPNTIIFLTADAFGVIPPISKLNKDQAMYHFLSGFTSKLAGTERGVTEPEPSFSTCFGAPFFPLHPTVYADLLGELIDLHDVDVYLVNTGWTGGKYGVGRRISLHYTRQMVNQAISGKLKNAEYTKDSTFGLSIP 474

HSP 2 Score: 36.1946 bits (82), Expect = 8.915e-50
Identity = 21/62 (33.87%), Postives = 33/62 (53.23%), Query Frame = 2

Query:  704 WGGKSPLKSKGVPSEILDPKNIWANKQVYKGNLVKLVGFFKRTF*YF-PT*KLAREKGSLKK 886
            +G   P++ + VP  IL+P N W++K+ YK     L+  F++ F  F    +   EKGS  K
Sbjct:  469 FGLSIPVEIEDVPKTILNPINAWSDKEKYKAQAEDLIQRFEKNFEKFGEKVEHIAEKGSFNK 530

BLAST of EY685008 vs. ExPASy Swiss-Prot
Match: PCKA_STAA2 (Phosphoenolpyruvate carboxykinase [ATP] OS=Staphylococcus aureus (strain JH1) GN=pckA PE=3 SV=1)

HSP 1 Score: 181.03 bits (458), Expect = 8.915e-50
Identity = 85/157 (54.14%), Postives = 105/157 (66.88%), Query Frame = 3

Query:  249 ENTRAAYPIEYIPNAKIPCVGPHPKNVILLACDAFGVLPPVSKLNLAQTMYHFISGYTALVAGTEDGIKEPTATFSACFGAAFIMLHPTKYAAMLAEKMQKHGATGWLVNTGWSGGSYGCGSRIKLPYTRKIIDGIHSGSLLNANYKKTDGFGVGNP 719
            ENTRAAYPI +I N  +P    HP  +I L  DAFGV+PP+SKLN  Q MYHF+SG+T+ +AGTE G+ EP  +FS CFGA F  LHPT YA +L E +  H    +LVNTGW+GG YG G RI L YTR++++   SG L NA Y K   FG+  P
Sbjct:  318 ENTRAAYPINHIDNIVVPSKAAHPNTIIFLTADAFGVIPPISKLNKDQAMYHFLSGFTSKLAGTERGVTEPEPSFSTCFGAPFFPLHPTVYADLLGELIDLHDVDVYLVNTGWTGGKYGVGRRISLHYTRQMVNQAISGKLKNAEYTKDSTFGLSIP 474

HSP 2 Score: 36.1946 bits (82), Expect = 8.915e-50
Identity = 21/62 (33.87%), Postives = 33/62 (53.23%), Query Frame = 2

Query:  704 WGGKSPLKSKGVPSEILDPKNIWANKQVYKGNLVKLVGFFKRTF*YF-PT*KLAREKGSLKK 886
            +G   P++ + VP  IL+P N W++K+ YK     L+  F++ F  F    +   EKGS  K
Sbjct:  469 FGLSIPVEIEDVPKTILNPINAWSDKEKYKAQAEDLIQRFEKNFEKFGEKVEHIAEKGSFNK 530

BLAST of EY685008 vs. ExPASy Swiss-Prot
Match: PCKA_STAA1 (Phosphoenolpyruvate carboxykinase [ATP] OS=Staphylococcus aureus (strain Mu3 / ATCC 700698) GN=pckA PE=3 SV=1)

HSP 1 Score: 181.03 bits (458), Expect = 8.915e-50
Identity = 85/157 (54.14%), Postives = 105/157 (66.88%), Query Frame = 3

Query:  249 ENTRAAYPIEYIPNAKIPCVGPHPKNVILLACDAFGVLPPVSKLNLAQTMYHFISGYTALVAGTEDGIKEPTATFSACFGAAFIMLHPTKYAAMLAEKMQKHGATGWLVNTGWSGGSYGCGSRIKLPYTRKIIDGIHSGSLLNANYKKTDGFGVGNP 719
            ENTRAAYPI +I N  +P    HP  +I L  DAFGV+PP+SKLN  Q MYHF+SG+T+ +AGTE G+ EP  +FS CFGA F  LHPT YA +L E +  H    +LVNTGW+GG YG G RI L YTR++++   SG L NA Y K   FG+  P
Sbjct:  318 ENTRAAYPINHIDNIVVPSKAAHPNTIIFLTADAFGVIPPISKLNKDQAMYHFLSGFTSKLAGTERGVTEPEPSFSTCFGAPFFPLHPTVYADLLGELIDLHDVDVYLVNTGWTGGKYGVGRRISLHYTRQMVNQAISGKLKNAEYTKDSTFGLSIP 474

HSP 2 Score: 36.1946 bits (82), Expect = 8.915e-50
Identity = 21/62 (33.87%), Postives = 33/62 (53.23%), Query Frame = 2

Query:  704 WGGKSPLKSKGVPSEILDPKNIWANKQVYKGNLVKLVGFFKRTF*YF-PT*KLAREKGSLKK 886
            +G   P++ + VP  IL+P N W++K+ YK     L+  F++ F  F    +   EKGS  K
Sbjct:  469 FGLSIPVEIEDVPKTILNPINAWSDKEKYKAQAEDLIQRFEKNFEKFGEKVEHIAEKGSFNK 530

BLAST of EY685008 vs. ExPASy Swiss-Prot
Match: PCKA_LYSSC (Phosphoenolpyruvate carboxykinase [ATP] OS=Lysinibacillus sphaericus (strain C3-41) GN=pckA PE=3 SV=1)

HSP 1 Score: 176.407 bits (446), Expect = 8.915e-50
Identity = 84/159 (52.83%), Postives = 105/159 (66.04%), Query Frame = 3

Query:  243 IAENTRAAYPIEYIPNAKIPCVGPHPKNVILLACDAFGVLPPVSKLNLAQTMYHFISGYTALVAGTEDGIKEPTATFSACFGAAFIMLHPTKYAAMLAEKMQKHGATGWLVNTGWSGGSYGCGSRIKLPYTRKIIDGIHSGSLLNANYKKTDGFGVGNP 719
            + ENTR AYPI+YI N   P V  HPK +I L  DAFGVLPP+SKL   Q MYHF+SG+T+ +AGTE G+ EP   FS CFG+ F+ L  T YA ML +K+ +HGA  +LVNTGW+GG YG GSR+KL YTR ++     G L N    +   FG+  P
Sbjct:  314 LTENTRVAYPIQYIENIVDPSVAGHPKTIIFLTADAFGVLPPISKLTKEQAMYHFLSGFTSKLAGTERGVTEPEPVFSTCFGSPFLPLPATVYAEMLGQKIDEHGAQVYLVNTGWTGGEYGTGSRMKLSYTRTMVRAAIDGKLTNVETTQDAVFGLHIP 472

HSP 2 Score: 40.817 bits (94), Expect = 8.915e-50
Identity = 16/45 (35.56%), Postives = 25/45 (55.56%), Query Frame = 2

Query:  701 IWGGKSPLKSKGVPSEILDPKNIWANKQVYKGNLVKLVGFFKRTF 835
            ++G   P   +GVPS++L+P+  WA+K  Y     +L G F   F
Sbjct:  466 VFGLHIPTAVEGVPSQVLNPREAWADKAAYDAKAAELAGLFNENF 510

BLAST of EY685008 vs. ExPASy Swiss-Prot
Match: PCKA_BACC2 (Phosphoenolpyruvate carboxykinase [ATP] OS=Bacillus cereus (strain G9842) GN=pckA PE=3 SV=1)

HSP 1 Score: 174.481 bits (441), Expect = 8.915e-50
Identity = 84/159 (52.83%), Postives = 105/159 (66.04%), Query Frame = 3

Query:  243 IAENTRAAYPIEYIPNAKIPCVGPHPKNVILLACDAFGVLPPVSKLNLAQTMYHFISGYTALVAGTEDGIKEPTATFSACFGAAFIMLHPTKYAAMLAEKMQKHGATGWLVNTGWSGGSYGCGSRIKLPYTRKIIDGIHSGSLLNANYKKTDGFGVGNP 719
            + ENTRAAYP+  I N  +P V  HP  +I L  DA GVLPP+SKL+  Q MYHF+SGYT+ +AGTE G+  P ATFS CFG+ F+ L  ++YA ML EK++KH A  +LVNTGW+GG YG G R+ L YTR +I    SG L      K D FG+  P
Sbjct:  314 LTENTRAAYPMHAIDNIVLPSVAGHPNTIIFLTADASGVLPPISKLSKEQAMYHFLSGYTSKLAGTERGVTSPQATFSTCFGSPFLPLDASRYAEMLGEKIEKHDAKVFLVNTGWTGGEYGVGKRMNLGYTRAMIQAALSGELAKTETAKHDIFGLEVP 472

HSP 2 Score: 42.743 bits (99), Expect = 8.915e-50
Identity = 19/45 (42.22%), Postives = 26/45 (57.78%), Query Frame = 2

Query:  701 IWGGKSPLKSKGVPSEILDPKNIWANKQVYKGNLVKLVGFFKRTF 835
            I+G + PL   GVP E+L P+  WA+K  YK   ++L   FK  F
Sbjct:  466 IFGLEVPLHVPGVPDEVLMPEQTWADKAAYKAKAIELANEFKENF 510

BLAST of EY685008 vs. ExPASy Swiss-Prot
Match: PCKA_BACHK (Phosphoenolpyruvate carboxykinase [ATP] OS=Bacillus thuringiensis subsp. konkukian GN=pckA PE=3 SV=1)

HSP 1 Score: 174.481 bits (441), Expect = 1.162e-49
Identity = 84/159 (52.83%), Postives = 105/159 (66.04%), Query Frame = 3

Query:  243 IAENTRAAYPIEYIPNAKIPCVGPHPKNVILLACDAFGVLPPVSKLNLAQTMYHFISGYTALVAGTEDGIKEPTATFSACFGAAFIMLHPTKYAAMLAEKMQKHGATGWLVNTGWSGGSYGCGSRIKLPYTRKIIDGIHSGSLLNANYKKTDGFGVGNP 719
            + ENTRAAYP+  I N  +P V  HP  +I L  DA GVLPP+SKL+  Q MYHF+SGYT+ +AGTE G+  P ATFS CFG+ F+ L  ++YA ML EK++KH A  +LVNTGW+GG YG G R+ L YTR +I    SG L      K D FG+  P
Sbjct:  314 LTENTRAAYPMHAIDNIVLPSVAGHPNTIIFLTADASGVLPPISKLSKEQAMYHFLSGYTSKLAGTERGVTSPQATFSTCFGSPFLPLDASRYAEMLGEKIEKHDAKVFLVNTGWTGGEYGVGKRMNLGYTRAMIQAALSGELAKTETAKHDIFGLEVP 472

HSP 2 Score: 42.3578 bits (98), Expect = 1.162e-49
Identity = 19/45 (42.22%), Postives = 26/45 (57.78%), Query Frame = 2

Query:  701 IWGGKSPLKSKGVPSEILDPKNIWANKQVYKGNLVKLVGFFKRTF 835
            I+G + PL   GVP E+L P+  WA+K  YK   ++L   FK  F
Sbjct:  466 IFGLEVPLHVPGVPDEVLMPEQTWADKAAYKAKAIELANEFKANF 510

BLAST of EY685008 vs. ExPASy Swiss-Prot
Match: PCKA_BACCQ (Phosphoenolpyruvate carboxykinase [ATP] OS=Bacillus cereus (strain Q1) GN=pckA PE=3 SV=1)

HSP 1 Score: 174.481 bits (441), Expect = 1.162e-49
Identity = 84/159 (52.83%), Postives = 105/159 (66.04%), Query Frame = 3

Query:  243 IAENTRAAYPIEYIPNAKIPCVGPHPKNVILLACDAFGVLPPVSKLNLAQTMYHFISGYTALVAGTEDGIKEPTATFSACFGAAFIMLHPTKYAAMLAEKMQKHGATGWLVNTGWSGGSYGCGSRIKLPYTRKIIDGIHSGSLLNANYKKTDGFGVGNP 719
            + ENTRAAYP+  I N  +P V  HP  +I L  DA GVLPP+SKL+  Q MYHF+SGYT+ +AGTE G+  P ATFS CFG+ F+ L  ++YA ML EK++KH A  +LVNTGW+GG YG G R+ L YTR +I    SG L      K D FG+  P
Sbjct:  314 LTENTRAAYPMHAIDNIVLPSVAGHPNTIIFLTADASGVLPPISKLSKEQAMYHFLSGYTSKLAGTERGVTSPQATFSTCFGSPFLPLDASRYAEMLGEKIEKHDAKVFLVNTGWTGGEYGVGKRMNLGYTRAMIQAALSGELAKTETAKHDIFGLEVP 472

HSP 2 Score: 42.3578 bits (98), Expect = 1.162e-49
Identity = 19/45 (42.22%), Postives = 26/45 (57.78%), Query Frame = 2

Query:  701 IWGGKSPLKSKGVPSEILDPKNIWANKQVYKGNLVKLVGFFKRTF 835
            I+G + PL   GVP E+L P+  WA+K  YK   ++L   FK  F
Sbjct:  466 IFGLEVPLHVPGVPDEVLMPEQTWADKAAYKAKAIELANEFKANF 510

BLAST of EY685008 vs. ExPASy Swiss-Prot
Match: PCKA_BACC7 (Phosphoenolpyruvate carboxykinase [ATP] OS=Bacillus cereus (strain AH187) GN=pckA PE=3 SV=1)

HSP 1 Score: 174.481 bits (441), Expect = 1.162e-49
Identity = 84/159 (52.83%), Postives = 105/159 (66.04%), Query Frame = 3

Query:  243 IAENTRAAYPIEYIPNAKIPCVGPHPKNVILLACDAFGVLPPVSKLNLAQTMYHFISGYTALVAGTEDGIKEPTATFSACFGAAFIMLHPTKYAAMLAEKMQKHGATGWLVNTGWSGGSYGCGSRIKLPYTRKIIDGIHSGSLLNANYKKTDGFGVGNP 719
            + ENTRAAYP+  I N  +P V  HP  +I L  DA GVLPP+SKL+  Q MYHF+SGYT+ +AGTE G+  P ATFS CFG+ F+ L  ++YA ML EK++KH A  +LVNTGW+GG YG G R+ L YTR +I    SG L      K D FG+  P
Sbjct:  314 LTENTRAAYPMHAIDNIVLPSVAGHPNTIIFLTADASGVLPPISKLSKEQAMYHFLSGYTSKLAGTERGVTSPQATFSTCFGSPFLPLDASRYAEMLGEKIEKHDAKVFLVNTGWTGGEYGVGKRMNLGYTRAMIQAALSGELAKTETAKHDIFGLEVP 472

HSP 2 Score: 42.3578 bits (98), Expect = 1.162e-49
Identity = 19/45 (42.22%), Postives = 26/45 (57.78%), Query Frame = 2

Query:  701 IWGGKSPLKSKGVPSEILDPKNIWANKQVYKGNLVKLVGFFKRTF 835
            I+G + PL   GVP E+L P+  WA+K  YK   ++L   FK  F
Sbjct:  466 IFGLEVPLHVPGVPDEVLMPEQTWADKAAYKAKAIELANEFKANF 510

The following BLAST results are available for this feature:

BLAST of EY685008 vs. ExPASy Swiss-Prot

BLAST of EY685008 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 284


Match Name	E-value	Identity	Description
PCKA_STAA9	8.915e-50	54.14	Phosphoenolpyruvate carboxykinase [ATP] OS=Staphyl...	[more]
PCKA_STAA8	8.915e-50	54.14	Phosphoenolpyruvate carboxykinase [ATP] OS=Staphyl...	[more]
PCKA_STAA3	8.915e-50	54.14	Phosphoenolpyruvate carboxykinase [ATP] OS=Staphyl...	[more]
PCKA_STAA2	8.915e-50	54.14	Phosphoenolpyruvate carboxykinase [ATP] OS=Staphyl...	[more]
PCKA_STAA1	8.915e-50	54.14	Phosphoenolpyruvate carboxykinase [ATP] OS=Staphyl...	[more]
PCKA_LYSSC	8.915e-50	52.83	Phosphoenolpyruvate carboxykinase [ATP] OS=Lysinib...	[more]
PCKA_BACC2	8.915e-50	52.83	Phosphoenolpyruvate carboxykinase [ATP] OS=Bacillu...	[more]
PCKA_BACHK	1.162e-49	52.83	Phosphoenolpyruvate carboxykinase [ATP] OS=Bacillu...	[more]
PCKA_BACCQ	1.162e-49	52.83	Phosphoenolpyruvate carboxykinase [ATP] OS=Bacillu...	[more]
PCKA_BACC7	1.162e-49	52.83	Phosphoenolpyruvate carboxykinase [ATP] OS=Bacillu...	[more]

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Properties


Property Name	Value
Genbank description	CS00-C2-003-007-E05-CT.F Sweet orange bark, greenhouse plant Citrus sinensis cDNA, mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>EY685008 ID=EY685008; Name=EY685008; organism=Citrus sinensis; type=EST; length=909bp
TTTAAGTCAATAGCTTAGCTATTTTGACTTTTTCTATGCCATGTTATAAG
GACTTGAACAAAAAGGGGTCTCCAAATATATAAGACTAAGCGAAAAACTT
AAATACTTAAGTGACACCGAAAAGTCTTTTGATCGTGATCTAAAGATCCA
GGAGAATTTATTTAATCTACAGATAAGCTATACCTACATATGCTCTATGA
TCGTGAAGAAAATTTATTTATCTTGTGGCTGTTTTTCATGGGATTGCAGA
GAACACTCGTGCTGCCTACCCCATTGAGTATATCCCCAATGCAAAGATAC
CATGCGTTGGTCCTCATCCCAAGAATGTCATCCTTTTGGCATGTGATGCT
TTTGGTGTACTCCCACCAGTGAGCAAGCTGAACCTGGCACAAACAATGTA
CCATTTCATCAGTGGCTACACTGCTTTGGTAGCTGGAACAGAGGATGGTA
TTAAGGAGCCAACGGCAACATTTTCAGCTTGCTTTGGTGCAGCATTTATC
ATGCTGCATCCTACCAAGTATGCAGCAATGCTGGCTGAGAAGATGCAGAA
GCATGGTGCTACAGGATGGCTCGTCAACACTGGATGGTCAGGTGGAAGCT
ATGGTTGTGGCAGCCGCATCAAGTTACCGTACACTCGNAAAATCATTGAT
GGGATACACTCTGGCAGCCTGTTGAATGCAAATTACAAGAAAACTGATGG
ATTTGGGGTGGGAAATCCCCACTGAAGTCGAAGGGAGTGCCTTCAGAAAT
CTTGGATCCAAAAAACATTTGGGCAAACAAGCAGGTTTACAAGGGAAACT
TGGTTAAGTTGGTTGGTTTTTTCAAAAGGACTTTTTAATATTTCCCAACT
TAAAAATTGGCAAGGGAAAAAGGTTCCTTGAAGAAATCCTTGGAGCCTGG
CTCCATTTT