EY685008

Overview
NameEY685008
Unique NameEY685008
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length909
Libraries
Library NameType
Sweet orange bark, greenhouse plantcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of EY685008 vs. ExPASy Swiss-Prot
Match: PCKA_RHOPT (Phosphoenolpyruvate carboxykinase [ATP] OS=Rhodopseudomonas palustris (strain TIE-1) GN=pckA PE=3 SV=1)

HSP 1 Score: 172.94 bits (437), Expect = 4.371e-49
Identity = 82/157 (52.23%), Postives = 103/157 (65.61%), Query Frame = 3 
Query:  249 ENTRAAYPIEYIPNAKIPCVGPHPKNVILLACDAFGVLPPVSKLNLAQTMYHFISGYTALVAGTEDGIKEPTATFSACFGAAFIMLHPTKYAAMLAEKMQKHGATGWLVNTGWSGGSYGCGSRIKLPYTRKIIDGIHSGSLLNANYKKTDGFGVGNP 719
            ENTR+AYP+E IPNA +      PKNV++LA DAFGV+PP++KL  AQ MYHF+SGYTA VAGTE G+ EPT  FS CFG+ F+   P+ Y  ML E + KH    WLVNTGW+GG YG G R+ +  TR ++     GSL N  ++    FG   P
Sbjct:  320 ENTRSAYPLESIPNASLTGRAGQPKNVVMLAADAFGVMPPIAKLTPAQAMYHFLSGYTAKVAGTERGVTEPTPEFSTCFGSPFLPRDPSVYGNMLRELIAKHNVDCWLVNTGWTGGIYGTGHRMPIKVTRALLTAALDGSLRNVEFRTDPYFGFAVP 476

HSP 2 Score: 41.9726 bits (97), Expect = 4.371e-49
Identity = 20/44 (45.45%), Postives = 25/44 (56.82%), Query Frame = 2 
Query:  704 WGGKSPLKSKGVPSEILDPKNIWANKQVYKGNLVKLVGFFKRTF 835
            +G   P    GVPSEILDP   WA+K  +     KLVG F++ F
Sbjct:  471 FGFAVPTALPGVPSEILDPVKTWADKAAFDTTARKLVGMFQKNF 514
[Close]
BLAST of EY685008 vs. ExPASy Swiss-Prot
Match: PCKA_RHOPA (Phosphoenolpyruvate carboxykinase [ATP] OS=Rhodopseudomonas palustris GN=pckA PE=1 SV=2)

HSP 1 Score: 172.94 bits (437), Expect = 4.371e-49
Identity = 82/157 (52.23%), Postives = 103/157 (65.61%), Query Frame = 3 
Query:  249 ENTRAAYPIEYIPNAKIPCVGPHPKNVILLACDAFGVLPPVSKLNLAQTMYHFISGYTALVAGTEDGIKEPTATFSACFGAAFIMLHPTKYAAMLAEKMQKHGATGWLVNTGWSGGSYGCGSRIKLPYTRKIIDGIHSGSLLNANYKKTDGFGVGNP 719
            ENTR+AYP+E IPNA +      PKNV++LA DAFGV+PP++KL  AQ MYHF+SGYTA VAGTE G+ EPT  FS CFG+ F+   P+ Y  ML E + KH    WLVNTGW+GG YG G R+ +  TR ++     GSL N  ++    FG   P
Sbjct:  320 ENTRSAYPLESIPNASLTGRAGQPKNVVMLAADAFGVMPPIAKLTPAQAMYHFLSGYTAKVAGTERGVTEPTPEFSTCFGSPFLPRDPSVYGNMLRELIAKHNVDCWLVNTGWTGGIYGTGHRMPIKVTRALLTAALDGSLRNVEFRTDPYFGFAVP 476

HSP 2 Score: 41.9726 bits (97), Expect = 4.371e-49
Identity = 20/44 (45.45%), Postives = 25/44 (56.82%), Query Frame = 2 
Query:  704 WGGKSPLKSKGVPSEILDPKNIWANKQVYKGNLVKLVGFFKRTF 835
            +G   P    GVPSEILDP   WA+K  +     KLVG F++ F
Sbjct:  471 FGFAVPTALPGVPSEILDPVKTWADKAAFDTTARKLVGMFQKNF 514
[Close]
BLAST of EY685008 vs. ExPASy Swiss-Prot
Match: PCKA_SINMW (Phosphoenolpyruvate carboxykinase [ATP] OS=Sinorhizobium medicae (strain WSM419) GN=pckA PE=3 SV=1)

HSP 1 Score: 181.415 bits (459), Expect = 5.697e-49
Identity = 82/159 (51.57%), Postives = 109/159 (68.55%), Query Frame = 3 
Query:  243 IAENTRAAYPIEYIPNAKIPCVGPHPKNVILLACDAFGVLPPVSKLNLAQTMYHFISGYTALVAGTEDGIKEPTATFSACFGAAFIMLHPTKYAAMLAEKMQKHGATGWLVNTGWSGGSYGCGSRIKLPYTRKIIDGIHSGSLLNANYKKTDGFGVGNP 719
            + ENTR AYP+ +IPNA      P P+ +I+L  DAFGVLPP++KL   Q MYHF+SGYTA VAGTE G+ EP ATFS CFGA F+  HP++Y  +L E + ++G T WLVNTGW+GG+YG GSR+ +  TR ++     GSL +A+++    FG   P
Sbjct:  317 LTENTRCAYPLHFIPNASKTGTAPQPRTIIMLTADAFGVLPPIAKLTPEQAMYHFLSGYTAKVAGTEKGVTEPEATFSTCFGAPFMPRHPSEYGNLLKELIARNGVTCWLVNTGWTGGAYGTGSRMPIKVTRALLSAALDGSLNSASFRSDANFGFAVP 475

HSP 2 Score: 33.113 bits (74), Expect = 5.697e-49
Identity = 16/44 (36.36%), Postives = 22/44 (50.00%), Query Frame = 2 
Query:  704 WGGKSPLKSKGVPSEILDPKNIWANKQVYKGNLVKLVGFFKRTF 835
            +G   P+   GV S ILDP++ WA+   Y     +LV  F   F
Sbjct:  470 FGFAVPVSVPGVESRILDPRSTWADGAAYDAQARRLVDMFIANF 513
[Close]
BLAST of EY685008 vs. ExPASy Swiss-Prot
Match: PCKA_BRASO (Phosphoenolpyruvate carboxykinase [ATP] OS=Bradyrhizobium sp. (strain ORS278) GN=pckA PE=3 SV=1)

HSP 1 Score: 175.637 bits (444), Expect = 5.697e-49
Identity = 84/158 (53.16%), Postives = 106/158 (67.09%), Query Frame = 3 
Query:  249 ENTRAAYPIEYIPNAKIPCVGPHPKNVILLACDAFGVLPPVSKLNLAQTMYHFISGYTALVAGTEDGI-KEPTATFSACFGAAFIMLHPTKYAAMLAEKMQKHGATGWLVNTGWSGGSYGCGSRIKLPYTRKIIDGIHSGSLLNANYKKTDGFGVGNP 719
            ENTR+AYP++YIPNA      PHPKNV++LA DAFGVLPP++KL+ AQ MYHF+SGYTA VAGTE G+  EP   FS CFG+ F+ L P+ Y  ML + +  H    WLVNTGW+GG YG GSR+ +  TR ++     GSL N  ++    FG   P
Sbjct:  319 ENTRSAYPLDYIPNASRTGRAPHPKNVVMLAADAFGVLPPIAKLSPAQAMYHFLSGYTAKVAGTERGLGSEPQPEFSTCFGSPFLPLDPSVYGNMLRQLIADHNVDCWLVNTGWTGGKYGTGSRMPIKVTRALLTAALDGSLRNVEFRTDKYFGFAVP 476

HSP 2 Score: 38.891 bits (89), Expect = 5.697e-49
Identity = 19/44 (43.18%), Postives = 24/44 (54.55%), Query Frame = 2 
Query:  704 WGGKSPLKSKGVPSEILDPKNIWANKQVYKGNLVKLVGFFKRTF 835
            +G   P    GVPSEILDP N W +K  +      LVG F++ F
Sbjct:  471 FGFAVPTALPGVPSEILDPVNTWKDKVEFDKTARALVGMFQKNF 514
[Close]
BLAST of EY685008 vs. ExPASy Swiss-Prot
Match: PCKA_PARL1 (Phosphoenolpyruvate carboxykinase [ATP] OS=Parvibaculum lavamentivorans (strain DS-1 / DSM 13023 / NCIMB 13966) GN=pckA PE=3 SV=1)

HSP 1 Score: 179.489 bits (454), Expect = 7.425e-49
Identity = 82/159 (51.57%), Postives = 108/159 (67.92%), Query Frame = 3 
Query:  243 IAENTRAAYPIEYIPNAKIPCVGPHPKNVILLACDAFGVLPPVSKLNLAQTMYHFISGYTALVAGTEDGIKEPTATFSACFGAAFIMLHPTKYAAMLAEKMQKHGATGWLVNTGWSGGSYGCGSRIKLPYTRKIIDGIHSGSLLNANYKKTDGFGVGNP 719
            +AEN+R AYP+ +IPNA      PHPKN+I+L  DAF VLPPV++L  +Q MYHF+SGYTA VAGTE G+ EP ATFS CFGA F+  HPT+Y  +L + + +H  + WLVNTGW+GG YG G+R+ +  TR ++     GSL N  ++    FG   P
Sbjct:  318 LAENSRGAYPLSFIPNASATGRAPHPKNIIMLTADAFSVLPPVARLTPSQAMYHFLSGYTAKVAGTEKGVTEPEATFSTCFGAPFMSRHPTEYGNLLRDLIAQHKVSCWLVNTGWTGGVYGTGNRMPIKATRALLAAALDGSLNNVEFRTDPNFGFEVP 476

HSP 2 Score: 34.6538 bits (78), Expect = 7.425e-49
Identity = 16/44 (36.36%), Postives = 24/44 (54.55%), Query Frame = 2 
Query:  704 WGGKSPLKSKGVPSEILDPKNIWANKQVYKGNLVKLVGFFKRTF 835
            +G + P+   GV ++IL+P+  WA+K  Y     KLV  F   F
Sbjct:  471 FGFEVPVDVPGVDNKILNPRETWADKAAYDAQAQKLVKMFIENF 514
[Close]
BLAST of EY685008 vs. ExPASy Swiss-Prot
Match: PCKA_CAMLR (Phosphoenolpyruvate carboxykinase [ATP] OS=Campylobacter lari (strain RM2100 / D67 / ATCC BAA-1060) GN=pckA PE=3 SV=1)

HSP 1 Score: 182.185 bits (461), Expect = 7.427e-49
Identity = 88/157 (56.05%), Postives = 108/157 (68.79%), Query Frame = 3 
Query:  249 ENTRAAYPIEYIPNAKIPCVGPHPKNVILLACDAFGVLPPVSKLNLAQTMYHFISGYTALVAGTEDGIKEPTATFSACFGAAFIMLHPTKYAAMLAEKMQKHGATGWLVNTGWSGGSYGCGSRIKLPYTRKIIDGIHSGSLLNANYKKTDGFGVGNP 719
            ENTR +YPIE+I N +      HP N+I L+ DAFGVLPPVSKL+  Q MY+F+SGYTA VAGTE GI EP ATFSACFG  F+ LHPT YA +L EK+ KH    +LVNTGWSGG+YG G R+ +  TR  I+ I  GS+ N  ++  D F +  P
Sbjct:  311 ENTRVSYPIEHILNHEPSLSAGHPSNIIFLSADAFGVLPPVSKLSKEQAMYYFLSGYTAKVAGTERGITEPVATFSACFGEVFLPLHPTVYAKLLGEKISKHNVNVYLVNTGWSGGAYGVGKRMSIKATRACINAILDGSIQNCEFENYDLFNLAVP 467

HSP 2 Score: 31.9574 bits (71), Expect = 7.427e-49
Identity = 14/39 (35.90%), Postives = 22/39 (56.41%), Query Frame = 2 
Query:  719 PLKSKGVPSEILDPKNIWANKQVYKGNLVKLVGFFKRTF 835
            P +  GV S++L+P N W +K+ Y+   +KL   F   F
Sbjct:  467 PKELAGVESKLLNPINTWEDKKAYEETKLKLAKMFIENF 505
[Close]
BLAST of EY685008 vs. ExPASy Swiss-Prot
Match: PCKA_THETN (Phosphoenolpyruvate carboxykinase [ATP] OS=Thermoanaerobacter tengcongensis GN=pckA PE=3 SV=1)

HSP 1 Score: 178.718 bits (452), Expect = 7.427e-49
Identity = 87/159 (54.72%), Postives = 106/159 (66.67%), Query Frame = 3 
Query:  243 IAENTRAAYPIEYIPNAKIPCVGPHPKNVILLACDAFGVLPPVSKLNLAQTMYHFISGYTALVAGTEDGIKEPTATFSACFGAAFIMLHPTKYAAMLAEKMQKHGATGWLVNTGWSGGSYGCGSRIKLPYTRKIIDGIHSGSLLNANYKKTDGFGVGNP 719
            I ENTRAAYPI++IP A    +G HPK +I L  DAFGVLPP++KL   Q MY+F+SGYT+ +AGTE GI EP ATFS CFGA F+ L P  YA ML EK++K+    +LVNTGWSGG YG G RI L YTRK++     G L    + K   F +  P
Sbjct:  308 ITENTRAAYPIDFIPGAVQSGIGGHPKTIIFLTADAFGVLPPIAKLTKEQAMYYFLSGYTSKLAGTERGITEPQATFSTCFGAPFLPLPPMVYAKMLGEKIEKYDTKVFLVNTGWSGGPYGIGKRINLEYTRKMVSAALKGELDKVEFTKDPIFNLNIP 466

HSP 2 Score: 35.4242 bits (80), Expect = 7.427e-49
Identity = 16/39 (41.03%), Postives = 21/39 (53.85%), Query Frame = 2 
Query:  719 PLKSKGVPSEILDPKNIWANKQVYKGNLVKLVGFFKRTF 835
            P    GVPSEIL+P+N W +K+ Y     +L   F   F
Sbjct:  466 PASCPGVPSEILNPRNTWKDKEEYDKTAKRLAQRFIENF 504
[Close]
BLAST of EY685008 vs. ExPASy Swiss-Prot
Match: PCKA_BRUME (Phosphoenolpyruvate carboxykinase [ATP] OS=Brucella melitensis GN=pckA PE=3 SV=2)

HSP 1 Score: 176.022 bits (445), Expect = 9.678e-49
Identity = 81/159 (50.94%), Postives = 107/159 (67.30%), Query Frame = 3 
Query:  243 IAENTRAAYPIEYIPNAKIPCVGPHPKNVILLACDAFGVLPPVSKLNLAQTMYHFISGYTALVAGTEDGIKEPTATFSACFGAAFIMLHPTKYAAMLAEKMQKHGATGWLVNTGWSGGSYGCGSRIKLPYTRKIIDGIHSGSLLNANYKKTDGFGVGNP 719
            + ENTR AYP+++IPNA     G  PKN+I+L  DAFGV+PP++KL  AQ MYHF+SGYTA VA TE G+ EP ATFS CFGA F+  HP++Y  +L + + +H    WLVNTGW+GG+YG G R+ +  TR ++     GSL NA ++    FG   P
Sbjct:  317 LTENTRCAYPLDFIPNASKSGKGGQPKNIIMLTADAFGVMPPIAKLTPAQAMYHFLSGYTAKVAATEKGVTEPEATFSTCFGAPFMPRHPSEYGNLLRKLIAEHKVDCWLVNTGWTGGAYGVGKRMPIKATRALLAAALDGSLNNAEFRIDPNFGFAVP 475

HSP 2 Score: 37.7354 bits (86), Expect = 9.678e-49
Identity = 18/44 (40.91%), Postives = 24/44 (54.55%), Query Frame = 2 
Query:  704 WGGKSPLKSKGVPSEILDPKNIWANKQVYKGNLVKLVGFFKRTF 835
            +G   P++  GV S ILDP++ WA+K  Y     KLV  F   F
Sbjct:  470 FGFAVPVEVPGVESSILDPRSTWADKVAYDAQAKKLVDMFVSNF 513
[Close]
BLAST of EY685008 vs. ExPASy Swiss-Prot
Match: PCKA_AGRRK (Phosphoenolpyruvate carboxykinase [ATP] OS=Agrobacterium radiobacter (strain K84 / ATCC BAA-868) GN=pckA PE=3 SV=1)

HSP 1 Score: 175.637 bits (444), Expect = 1.261e-48
Identity = 80/159 (50.31%), Postives = 108/159 (67.92%), Query Frame = 3 
Query:  243 IAENTRAAYPIEYIPNAKIPCVGPHPKNVILLACDAFGVLPPVSKLNLAQTMYHFISGYTALVAGTEDGIKEPTATFSACFGAAFIMLHPTKYAAMLAEKMQKHGATGWLVNTGWSGGSYGCGSRIKLPYTRKIIDGIHSGSLLNANYKKTDGFGVGNP 719
            + ENTR+AYP+++IPNA    +  HP+ +I+L  DAFGV+PP+++L   Q MYHF+SGYTA VAGTE G+ EP ATFS CFGA F+  HPT+Y  +L E + +HGA  WLVNTGW+GG+YG G R+ +  TR ++     G L  A ++    FG   P
Sbjct:  317 LTENTRSAYPLDFIPNASESGMTGHPETIIMLTADAFGVMPPIARLTPEQAMYHFLSGYTAKVAGTEKGVVEPEATFSTCFGAPFMPRHPTEYGNLLKELIARHGAGCWLVNTGWTGGAYGTGRRMPIKATRALLAAALKGDLDKAEFRIDPNFGFAVP 475

HSP 2 Score: 37.7354 bits (86), Expect = 1.261e-48
Identity = 18/44 (40.91%), Postives = 24/44 (54.55%), Query Frame = 2 
Query:  704 WGGKSPLKSKGVPSEILDPKNIWANKQVYKGNLVKLVGFFKRTF 835
            +G   P+  +GV S ILDP++ WA+ Q Y     KLV  F   F
Sbjct:  470 FGFAVPVAVEGVDSAILDPRSTWADGQAYDAQAAKLVQMFVSNF 513
[Close]
BLAST of EY685008 vs. ExPASy Swiss-Prot
Match: PCKA_OCHA4 (Phosphoenolpyruvate carboxykinase [ATP] OS=Ochrobactrum anthropi (strain ATCC 49188 / DSM 6882 / NCTC 12168) GN=pckA PE=3 SV=1)

HSP 1 Score: 176.407 bits (446), Expect = 2.143e-48
Identity = 81/159 (50.94%), Postives = 108/159 (67.92%), Query Frame = 3 
Query:  243 IAENTRAAYPIEYIPNAKIPCVGPHPKNVILLACDAFGVLPPVSKLNLAQTMYHFISGYTALVAGTEDGIKEPTATFSACFGAAFIMLHPTKYAAMLAEKMQKHGATGWLVNTGWSGGSYGCGSRIKLPYTRKIIDGIHSGSLLNANYKKTDGFGVGNP 719
            + ENTR AYP+++IPNA     G  PKN+I+L  DAFGV+PP++KL  AQ MYHF+SGYTA VAGTE G+ EP ATFS CFGA F+  HP++Y  +L + + +H    WLVNTGW+GG+YG G R+ +  TR ++     GSL +A ++    FG   P
Sbjct:  317 LTENTRCAYPLDFIPNASKSGKGGQPKNIIMLTADAFGVMPPIAKLTPAQAMYHFLSGYTAKVAGTEKGVTEPEATFSTCFGAPFMPRHPSEYGNLLRKLIAEHKVDCWLVNTGWTGGAYGVGKRMPIKATRALLAAALDGSLNDAEFRIDPNFGFAVP 475

HSP 2 Score: 36.1946 bits (82), Expect = 2.143e-48
Identity = 17/44 (38.64%), Postives = 23/44 (52.27%), Query Frame = 2 
Query:  704 WGGKSPLKSKGVPSEILDPKNIWANKQVYKGNLVKLVGFFKRTF 835
            +G   P+   GV + ILDP++ WA+K  Y     KLV  F   F
Sbjct:  470 FGFAVPVDVPGVDTSILDPRSTWADKAAYDAQAKKLVDMFVTNF 513
The following BLAST results are available for this feature:
BLAST of EY685008 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 284
Match NameE-valueIdentityDescription
PCKA_RHOPT4.371e-4952.23Phosphoenolpyruvate carboxykinase [ATP] OS=Rhodops... [more]
PCKA_RHOPA4.371e-4952.23Phosphoenolpyruvate carboxykinase [ATP] OS=Rhodops... [more]
PCKA_SINMW5.697e-4951.57Phosphoenolpyruvate carboxykinase [ATP] OS=Sinorhi... [more]
PCKA_BRASO5.697e-4953.16Phosphoenolpyruvate carboxykinase [ATP] OS=Bradyrh... [more]
PCKA_PARL17.425e-4951.57Phosphoenolpyruvate carboxykinase [ATP] OS=Parviba... [more]
PCKA_CAMLR7.427e-4956.05Phosphoenolpyruvate carboxykinase [ATP] OS=Campylo... [more]
PCKA_THETN7.427e-4954.72Phosphoenolpyruvate carboxykinase [ATP] OS=Thermoa... [more]
PCKA_BRUME9.678e-4950.94Phosphoenolpyruvate carboxykinase [ATP] OS=Brucell... [more]
PCKA_AGRRK1.261e-4850.31Phosphoenolpyruvate carboxykinase [ATP] OS=Agrobac... [more]
PCKA_OCHA42.143e-4850.94Phosphoenolpyruvate carboxykinase [ATP] OS=Ochroba... [more]

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Properties
Property NameValue
Genbank descriptionCS00-C2-003-007-E05-CT.F Sweet orange bark, greenhouse plant Citrus sinensis cDNA, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>EY685008 ID=EY685008; Name=EY685008; organism=Citrus sinensis; type=EST; length=909bp
TTTAAGTCAATAGCTTAGCTATTTTGACTTTTTCTATGCCATGTTATAAG
GACTTGAACAAAAAGGGGTCTCCAAATATATAAGACTAAGCGAAAAACTT
AAATACTTAAGTGACACCGAAAAGTCTTTTGATCGTGATCTAAAGATCCA
GGAGAATTTATTTAATCTACAGATAAGCTATACCTACATATGCTCTATGA
TCGTGAAGAAAATTTATTTATCTTGTGGCTGTTTTTCATGGGATTGCAGA
GAACACTCGTGCTGCCTACCCCATTGAGTATATCCCCAATGCAAAGATAC
CATGCGTTGGTCCTCATCCCAAGAATGTCATCCTTTTGGCATGTGATGCT
TTTGGTGTACTCCCACCAGTGAGCAAGCTGAACCTGGCACAAACAATGTA
CCATTTCATCAGTGGCTACACTGCTTTGGTAGCTGGAACAGAGGATGGTA
TTAAGGAGCCAACGGCAACATTTTCAGCTTGCTTTGGTGCAGCATTTATC
ATGCTGCATCCTACCAAGTATGCAGCAATGCTGGCTGAGAAGATGCAGAA
GCATGGTGCTACAGGATGGCTCGTCAACACTGGATGGTCAGGTGGAAGCT
ATGGTTGTGGCAGCCGCATCAAGTTACCGTACACTCGNAAAATCATTGAT
GGGATACACTCTGGCAGCCTGTTGAATGCAAATTACAAGAAAACTGATGG
ATTTGGGGTGGGAAATCCCCACTGAAGTCGAAGGGAGTGCCTTCAGAAAT
CTTGGATCCAAAAAACATTTGGGCAAACAAGCAGGTTTACAAGGGAAACT
TGGTTAAGTTGGTTGGTTTTTTCAAAAGGACTTTTTAATATTTCCCAACT
TAAAAATTGGCAAGGGAAAAAGGTTCCTTGAAGAAATCCTTGGAGCCTGG
CTCCATTTT
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