EY684565

Overview
NameEY684565
Unique NameEY684565
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length883
Libraries
Library NameType
Sweet orange bark, greenhouse plantcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of EY684565 vs. ExPASy Swiss-Prot
Match: RL2_METS3 (50S ribosomal protein L2P OS=Methanobrevibacter smithii (strain PS / ATCC 35061 / DSM 861) GN=rpl2p PE=3 SV=1)

HSP 1 Score: 169.474 bits (428), Expect = 2.312e-41
Identity = 92/214 (42.99%), Postives = 128/214 (59.81%), Query Frame = 1 
Query:   13 MGRVIRAQRKGAGSVFKSHTHHR-KGPAQFRSLDFGERNGYLKGVVAEIIHDPGRGAPLARVTFRHPFRYMKQKELFIAAEGMYTGQFVYCGKKATLMVGNVLPVRSIPEGAVVCNVEHHVGDRGVLARASGDYAVIISHNPDNDTTRVKLPSGAKKIIPSGCRAMIGQVAGGGRTEKPLLKAGNAYHQFRVKPNCWPKVRGVAMNPVEHPRGG 651
            MG+ +  QR+G G+       HR K   ++RS D  E+ G +KG+V +I+HDP R AP+A V F +      +K+  +A E +     + CG  A +  GN LP+  IPEG  + ++E+  GD G   R+SG YA +I+H  D + + V+LPSG  K +   CRA IG VAGGGR EKP LKAGN +H ++ K   +  VRGVAMN V+HP GG
Sbjct:    1 MGKRLIIQRRGRGTPAHRVASHRFKDKIRYRSYDALEKEGSIKGIVTDIVHDPARTAPIAEVKFENG-----EKKFILAPESIQIDDEIECGISAPIKFGNTLPLAEIPEGTPIYDIENTPGDGGRFVRSSGTYASLITH--DANQSVVELPSGELKYLNPRCRASIGVVAGGGRKEKPFLKAGNRWHAYKAKGKKFMTVRGVAMNAVDHPHGG 207
[Close]
BLAST of EY684565 vs. ExPASy Swiss-Prot
Match: RL2_METAC (50S ribosomal protein L2P OS=Methanosarcina acetivorans GN=rpl2p PE=3 SV=1)

HSP 1 Score: 154.451 bits (389), Expect = 4.793e-41
Identity = 83/215 (38.60%), Postives = 126/215 (58.60%), Query Frame = 1 
Query:   13 MGRVIRAQRKGAGS-VFKSHTHHRKGPAQFRSLDFGERNGYLKGVVAEIIHDPGRGAPLARVTFRHPFRYMKQKELFI-AAEGMYTGQFVYCGKKATLMVGNVLPVRSIPEGAVVCNVEHHVGDRGVLARASGDYAVIISHNPDNDTTRVKLPSGAKKIIPSGCRAMIGQVAGGGRTEKPLLKAGNAYHQFRVKPNCWPKVRGVAMNPVEHPRGG 651
            MG+ + +Q +G G+  +++ +H  K   +   +D    N  L+G V  I HDP R AP+A+V F +       +ELF+ A+EG+  G  + CG  A +  GN++P+ ++PEG  +CNVE    D G   R+SG YA +++H      T V +PSG  K +   CRA++G VAG GR ++P LKAG  YH+ + +   +P+V  VAMNP +HP GG
Sbjct:    1 MGKRLISQNRGRGTPTYRAPSHKYKADLRHPRVD---ENSSLRGEVVGIEHDPARSAPIAKVAFENG------EELFLLASEGIAVGNIIECGDDAEVKPGNIVPIGNVPEGFFICNVESKPNDGGKFVRSSGVYATVVTHEATR--TAVSMPSGNIKWLNPKCRAVVGIVAGSGRVDRPWLKAGKKYHKMKTRAAKYPRVSAVAMNPRDHPFGG 204

HSP 2 Score: 35.4242 bits (80), Expect = 4.793e-41
Identity = 18/39 (46.15%), Postives = 22/39 (56.41%), Query Frame = 3 
Query:  627 PGGASSWREDPQHMGHASTVRGDLPPASKVGLIAPRRIG 743
            P G  +W+    H G  +TV  + PP  KVGLIA RR G
Sbjct:  201 PFGGGAWK----HPGKPTTVSRNAPPGRKVGLIAARRTG 235
[Close]
BLAST of EY684565 vs. ExPASy Swiss-Prot
Match: RL8_ENTHI (60S ribosomal protein L8 (Fragment) OS=Entamoeba histolytica GN=RPL8 PE=2 SV=1)

HSP 1 Score: 168.318 bits (425), Expect = 5.150e-41
Identity = 76/114 (66.67%), Postives = 94/114 (82.46%), Query Frame = 1 
Query:  136 KGVVAEIIHDPGRGAPLARVTFRHPFRYMKQKELFIAAEGMYTGQFVYCGKKATLMVGNVLPVRSIPEGAVVCNVEHHVGDRGVLARASGDYAVIISHNPDNDTTRVKLPSGAK 477
            +G+V  I HDPGRGAPLARV FR+P +Y   KELFI AEG+Y+GQ++YCG KA+L +GNVLP+   PEG VVCNVE   GDRG++ARASG+YA +ISHN DN+TTR++LPSGAK
Sbjct:   33 RGLVKSIEHDPGRGAPLARVVFRNPRQYGLDKELFICAEGLYSGQYIYCGSKASLHIGNVLPIGQCPEGTVVCNVESKPGDRGIIARASGNYATVISHNADNETTRIRLPSGAK 146
[Close]
BLAST of EY684565 vs. ExPASy Swiss-Prot
Match: RL2_PYRAR (50S ribosomal protein L2P OS=Pyrobaculum arsenaticum (strain DSM 13514 / JCM 11321) GN=rpl2p PE=3 SV=1)

HSP 1 Score: 167.933 bits (424), Expect = 6.726e-41
Identity = 99/251 (39.44%), Postives = 135/251 (53.78%), Query Frame = 1 
Query:   13 MGRVIRAQRKG-AGSVFKSHTHHRKGPAQFRSLDFGERNGYLKGVVAEIIHDPGRGAPLARVTFRHPFRYMKQKELFIAAEGMYTGQFVYCGKKATLMVGNVLPVRSIPEGAVVCNVEHHVGDRGVLARASGDYAVIISHNPDNDTTRVKLPSGAKKIIPSGCRAMIGQVAGGGRTEKPLLKAGNAYHQFRVKPNCWPKVRGVAMNPVEHPRGGRTPSTWAMLALLGGIFPLPARWVLLHPGGLVASRVTG 762
            MG+ I  QR+G  GS F+S +  R GP ++  L+     GY    V E++H+PG  AP+AR+       +        AAEG+Y GQ +  G  AT   GN++ +  IPEG ++ NVE   GD G  AR+SG YAV++   P+ + T ++LPSG    + +  RA +G VAGGGR EKP LKAG  YH+ R K   +P VRG AM+P  HP GG +           G  P+P         G + SR TG
Sbjct:    1 MGKRILVQRRGRGGSQFRSPSWKRDGPVRYLPLEATSGRGY----VVELLHEPGLNAPVARIVTESGAEFFNY-----AAEGLYVGQVIEMGSGATPKAGNIMILGEIPEGTMIFNVEKRAGDGGKYARSSGTYAVVVGQKPEENKTIIRLPSGRVIEVDARGRATVGIVAGGGRIEKPFLKAGKKYHRARAKAWKYPTVRGKAMSPYAHPHGGGSHQK--------GGTPVPKTAPPGQKVGFIGSRCTG 234
[Close]
BLAST of EY684565 vs. ExPASy Swiss-Prot
Match: RL2_SULIY (50S ribosomal protein L2P OS=Sulfolobus islandicus (strain Y.G.57.14 / Yellowstone #1) GN=rpl2p PE=3 SV=1)

HSP 1 Score: 155.992 bits (393), Expect = 8.137e-41
Identity = 87/214 (40.65%), Postives = 127/214 (59.35%), Query Frame = 1 
Query:   13 MGRVIRAQRKGAGS-VFKSHTHHRKGPAQFRSLDFGERNGYLKGVVAEIIHDPGRGAPLARVTFRHPFRYMKQKELFIAAEGMYTGQFVYCGKKATLMVGNVLPVRSIPEGAVVCNVEHHVGDRGVLARASGDYAVIISHNPDNDTTRVKLPSGAKKIIPSGCRAMIGQVAGGGRTEKPLLKAGNAYHQFRVKPNCWPKVRGVAMNPVEHPRGG 651
            MG+ +  QR G GS  F+S +  R G  ++ ++      G+L G V +I+H+PG  AP+A +   +  +++ Q     A +G+   Q +  GK + +  GNV+ +   PEG +VCNVE + GD G  AR++G YAV++  +   D   +KLPS   K + +  RA +G VAGGG  EKPLLKAG  Y +++VK   WP VRGVAMN V+HP GG
Sbjct:    1 MGKNLLQQRAGKGSPTFRSPSWLRIGKVRYPNIF-----GHLVGKVIDIVHNPGMNAPVAIIKLENGTKFLTQ-----AIQGLVINQKIEFGKGSPIANGNVIEIGDAPEGTIVCNVEENFGDGGKYARSAGSYAVVVGKS--GDKVLIKLPSDKIKAVSNKARATVGVVAGGGVVEKPLLKAGANYWKYKVKAKKWPIVRGVAMNVVDHPHGG 202

HSP 2 Score: 33.113 bits (74), Expect = 8.137e-41
Identity = 17/32 (53.12%), Postives = 19/32 (59.38%), Query Frame = 3 
Query:  660 QHMGHASTVRGDLPPASKVGLIAPRRIGRFEG 755
            Q +   STV  + PP  KVG IA RR GR EG
Sbjct:  206 QSVSRPSTVSRNAPPGRKVGHIAARRTGRKEG 237
[Close]
BLAST of EY684565 vs. ExPASy Swiss-Prot
Match: RL2_SULIM (50S ribosomal protein L2P OS=Sulfolobus islandicus (strain M.14.25 / Kamchatka #1) GN=rpl2p PE=3 SV=1)

HSP 1 Score: 155.992 bits (393), Expect = 8.137e-41
Identity = 87/214 (40.65%), Postives = 127/214 (59.35%), Query Frame = 1 
Query:   13 MGRVIRAQRKGAGS-VFKSHTHHRKGPAQFRSLDFGERNGYLKGVVAEIIHDPGRGAPLARVTFRHPFRYMKQKELFIAAEGMYTGQFVYCGKKATLMVGNVLPVRSIPEGAVVCNVEHHVGDRGVLARASGDYAVIISHNPDNDTTRVKLPSGAKKIIPSGCRAMIGQVAGGGRTEKPLLKAGNAYHQFRVKPNCWPKVRGVAMNPVEHPRGG 651
            MG+ +  QR G GS  F+S +  R G  ++ ++      G+L G V +I+H+PG  AP+A +   +  +++ Q     A +G+   Q +  GK + +  GNV+ +   PEG +VCNVE + GD G  AR++G YAV++  +   D   +KLPS   K + +  RA +G VAGGG  EKPLLKAG  Y +++VK   WP VRGVAMN V+HP GG
Sbjct:    1 MGKNLLQQRAGKGSPTFRSPSWLRIGKVRYPNIF-----GHLVGKVIDIVHNPGMNAPVAIIKLENGTKFLTQ-----AIQGLVINQKIEFGKGSPIANGNVIEIGDAPEGTIVCNVEENFGDGGKYARSAGSYAVVVGKS--GDKVLIKLPSDKIKAVSNKARATVGVVAGGGVVEKPLLKAGANYWKYKVKAKKWPIVRGVAMNVVDHPHGG 202

HSP 2 Score: 33.113 bits (74), Expect = 8.137e-41
Identity = 17/32 (53.12%), Postives = 19/32 (59.38%), Query Frame = 3 
Query:  660 QHMGHASTVRGDLPPASKVGLIAPRRIGRFEG 755
            Q +   STV  + PP  KVG IA RR GR EG
Sbjct:  206 QSVSRPSTVSRNAPPGRKVGHIAARRTGRKEG 237
[Close]
BLAST of EY684565 vs. ExPASy Swiss-Prot
Match: RL2_SULIL (50S ribosomal protein L2P OS=Sulfolobus islandicus (strain L.S.2.15 / Lassen #1) GN=rpl2p PE=3 SV=1)

HSP 1 Score: 155.992 bits (393), Expect = 8.137e-41
Identity = 87/214 (40.65%), Postives = 127/214 (59.35%), Query Frame = 1 
Query:   13 MGRVIRAQRKGAGS-VFKSHTHHRKGPAQFRSLDFGERNGYLKGVVAEIIHDPGRGAPLARVTFRHPFRYMKQKELFIAAEGMYTGQFVYCGKKATLMVGNVLPVRSIPEGAVVCNVEHHVGDRGVLARASGDYAVIISHNPDNDTTRVKLPSGAKKIIPSGCRAMIGQVAGGGRTEKPLLKAGNAYHQFRVKPNCWPKVRGVAMNPVEHPRGG 651
            MG+ +  QR G GS  F+S +  R G  ++ ++      G+L G V +I+H+PG  AP+A +   +  +++ Q     A +G+   Q +  GK + +  GNV+ +   PEG +VCNVE + GD G  AR++G YAV++  +   D   +KLPS   K + +  RA +G VAGGG  EKPLLKAG  Y +++VK   WP VRGVAMN V+HP GG
Sbjct:    1 MGKNLLQQRAGKGSPTFRSPSWLRIGKVRYPNIF-----GHLVGKVIDIVHNPGMNAPVAIIKLENGTKFLTQ-----AIQGLVINQKIEFGKGSPIANGNVIEIGDAPEGTIVCNVEENFGDGGKYARSAGSYAVVVGKS--GDKVLIKLPSDKIKAVSNKARATVGVVAGGGVVEKPLLKAGANYWKYKVKAKKWPIVRGVAMNVVDHPHGG 202

HSP 2 Score: 33.113 bits (74), Expect = 8.137e-41
Identity = 17/32 (53.12%), Postives = 19/32 (59.38%), Query Frame = 3 
Query:  660 QHMGHASTVRGDLPPASKVGLIAPRRIGRFEG 755
            Q +   STV  + PP  KVG IA RR GR EG
Sbjct:  206 QSVSRPSTVSRNAPPGRKVGHIAARRTGRKEG 237
[Close]
BLAST of EY684565 vs. ExPASy Swiss-Prot
Match: RL2_SULIK (50S ribosomal protein L2P OS=Sulfolobus islandicus (strain M.16.4 / Kamchatka #3) GN=rpl2p PE=3 SV=1)

HSP 1 Score: 155.992 bits (393), Expect = 8.137e-41
Identity = 87/214 (40.65%), Postives = 127/214 (59.35%), Query Frame = 1 
Query:   13 MGRVIRAQRKGAGS-VFKSHTHHRKGPAQFRSLDFGERNGYLKGVVAEIIHDPGRGAPLARVTFRHPFRYMKQKELFIAAEGMYTGQFVYCGKKATLMVGNVLPVRSIPEGAVVCNVEHHVGDRGVLARASGDYAVIISHNPDNDTTRVKLPSGAKKIIPSGCRAMIGQVAGGGRTEKPLLKAGNAYHQFRVKPNCWPKVRGVAMNPVEHPRGG 651
            MG+ +  QR G GS  F+S +  R G  ++ ++      G+L G V +I+H+PG  AP+A +   +  +++ Q     A +G+   Q +  GK + +  GNV+ +   PEG +VCNVE + GD G  AR++G YAV++  +   D   +KLPS   K + +  RA +G VAGGG  EKPLLKAG  Y +++VK   WP VRGVAMN V+HP GG
Sbjct:    1 MGKNLLQQRAGKGSPTFRSPSWLRIGKVRYPNIF-----GHLVGKVIDIVHNPGMNAPVAIIKLENGTKFLTQ-----AIQGLVINQKIEFGKGSPIANGNVIEIGDAPEGTIVCNVEENFGDGGKYARSAGSYAVVVGKS--GDKVLIKLPSDKIKAVSNKARATVGVVAGGGVVEKPLLKAGANYWKYKVKAKKWPIVRGVAMNVVDHPHGG 202

HSP 2 Score: 33.113 bits (74), Expect = 8.137e-41
Identity = 17/32 (53.12%), Postives = 19/32 (59.38%), Query Frame = 3 
Query:  660 QHMGHASTVRGDLPPASKVGLIAPRRIGRFEG 755
            Q +   STV  + PP  KVG IA RR GR EG
Sbjct:  206 QSVSRPSTVSRNAPPGRKVGHIAARRTGRKEG 237
[Close]
BLAST of EY684565 vs. ExPASy Swiss-Prot
Match: RL2_SULIA (50S ribosomal protein L2P OS=Sulfolobus islandicus (strain M.16.27) GN=rpl2p PE=3 SV=1)

HSP 1 Score: 155.992 bits (393), Expect = 8.137e-41
Identity = 87/214 (40.65%), Postives = 127/214 (59.35%), Query Frame = 1 
Query:   13 MGRVIRAQRKGAGS-VFKSHTHHRKGPAQFRSLDFGERNGYLKGVVAEIIHDPGRGAPLARVTFRHPFRYMKQKELFIAAEGMYTGQFVYCGKKATLMVGNVLPVRSIPEGAVVCNVEHHVGDRGVLARASGDYAVIISHNPDNDTTRVKLPSGAKKIIPSGCRAMIGQVAGGGRTEKPLLKAGNAYHQFRVKPNCWPKVRGVAMNPVEHPRGG 651
            MG+ +  QR G GS  F+S +  R G  ++ ++      G+L G V +I+H+PG  AP+A +   +  +++ Q     A +G+   Q +  GK + +  GNV+ +   PEG +VCNVE + GD G  AR++G YAV++  +   D   +KLPS   K + +  RA +G VAGGG  EKPLLKAG  Y +++VK   WP VRGVAMN V+HP GG
Sbjct:    1 MGKNLLQQRAGKGSPTFRSPSWLRIGKVRYPNIF-----GHLVGKVIDIVHNPGMNAPVAIIKLENGTKFLTQ-----AIQGLVINQKIEFGKGSPIANGNVIEIGDAPEGTIVCNVEENFGDGGKYARSAGSYAVVVGKS--GDKVLIKLPSDKIKAVSNKARATVGVVAGGGVVEKPLLKAGANYWKYKVKAKKWPIVRGVAMNVVDHPHGG 202

HSP 2 Score: 33.113 bits (74), Expect = 8.137e-41
Identity = 17/32 (53.12%), Postives = 19/32 (59.38%), Query Frame = 3 
Query:  660 QHMGHASTVRGDLPPASKVGLIAPRRIGRFEG 755
            Q +   STV  + PP  KVG IA RR GR EG
Sbjct:  206 QSVSRPSTVSRNAPPGRKVGHIAARRTGRKEG 237
[Close]
BLAST of EY684565 vs. ExPASy Swiss-Prot
Match: RL2_IGNH4 (50S ribosomal protein L2P OS=Ignicoccus hospitalis (strain KIN4/I / DSM 18386 / JCM 14125) GN=rpl2p PE=3 SV=1)

HSP 1 Score: 166.777 bits (421), Expect = 1.498e-40
Identity = 93/214 (43.46%), Postives = 129/214 (60.28%), Query Frame = 1 
Query:   13 MGRVIRAQRKGAGSV-FKSHTHHRKGPAQFRSLDFGERNGYLKGVVAEIIHDPGRGAPLARVTFRHPFRYMKQKELFIAAEGMYTGQFVYCGKKATLMVGNVLPVRSIPEGAVVCNVEHHVGDRGVLARASGDYAVIISHNPDNDTTRVKLPSGAKKIIPSGCRAMIGQVAGGGRTEKPLLKAGNAYHQFRVKPNCWPKVRGVAMNPVEHPRGG 651
            MG+ +R QR G G+  +++  H R  P ++ S +  + +   +G V E+ HDPGR  PLA +   +   +        AAEGMYTGQ V  G  A  + GN+LP+ +IPEG  + NVE   GD G LAR+ G YA+++  +   ++  ++LPSG  K + +  RA IG  AG GR EKPLLKAGNAY++++ K   WP VRGVAMN V HP GG
Sbjct:    1 MGKRLRVQRHGRGTPQWRNRGHLRVAPGRYPSPEVLKLDETYEGYVVELKHDPGRWVPLAHIVIPNVADFW-----IPAAEGMYTGQKVQIGPNAAPVNGNILPLYAIPEGTQIFNVEIRPGDGGKLARSGGAYAILVGKS--GNSAIIQLPSGKVKQVNAKARATIGIAAGAGRDEKPLLKAGNAYYKWKAKAKKWPVVRGVAMNAVNHPHGG 207
The following BLAST results are available for this feature:
BLAST of EY684565 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 500
Match NameE-valueIdentityDescription
RL2_METS32.312e-4142.9950S ribosomal protein L2P OS=Methanobrevibacter sm... [more]
RL2_METAC4.793e-4138.6050S ribosomal protein L2P OS=Methanosarcina acetiv... [more]
RL8_ENTHI5.150e-4166.6760S ribosomal protein L8 (Fragment) OS=Entamoeba h... [more]
RL2_PYRAR6.726e-4139.4450S ribosomal protein L2P OS=Pyrobaculum arsenatic... [more]
RL2_SULIY8.137e-4140.6550S ribosomal protein L2P OS=Sulfolobus islandicus... [more]
RL2_SULIM8.137e-4140.6550S ribosomal protein L2P OS=Sulfolobus islandicus... [more]
RL2_SULIL8.137e-4140.6550S ribosomal protein L2P OS=Sulfolobus islandicus... [more]
RL2_SULIK8.137e-4140.6550S ribosomal protein L2P OS=Sulfolobus islandicus... [more]
RL2_SULIA8.137e-4140.6550S ribosomal protein L2P OS=Sulfolobus islandicus... [more]
RL2_IGNH41.498e-4043.4650S ribosomal protein L2P OS=Ignicoccus hospitalis... [more]

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Properties
Property NameValue
Genbank descriptionCS00-C2-003-001-F06-CT.F Sweet orange bark, greenhouse plant Citrus sinensis cDNA, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>EY684565 ID=EY684565; Name=EY684565; organism=Citrus sinensis; type=EST; length=883bp
CCACGCGTCCGAATGGGTCGTGTGATTCGCGCACAGCGTAAGGGTGCAGG
TTCGGTCTTCAAGTCCCACACCCACCACCGTAAGGGTCCGGCCCAGTTCC
GCAGCCTCGACTTCGGTGAGCGTAATGGTTACCTCAAGGGTGTCGTCGCA
GAGATTATTCACGATCCGGGTCGTGGTGCCCCGCTGGCCCGAGTTACCTT
TCGTCACCCTTTTCGCTACATGAAACAAAAGGAGCTTTTCATTGCCGCCG
AGGGTATGTACACGGGTCAGTTCGTGTACTGCGGCAAGAAAGCCACTCTG
ATGGTTGGTAATGTGTTGCCCGTTAGATCTATCCCTGAAGGAGCTGTCGT
TTGCAACGTTGAGCATCATGTTGGTGATCGCGGTGTTCTCGCTAGAGCTT
CTGGTGATTATGCCGTTATCATCAGTCATAACCCCGATAATGACACCACC
AGGGTCAAGCTTCCATCTGGTGCCAAAAAGATTATTCCAAGTGGCTGCAG
AGCTATGATTGGGCAGGTTGCCGGCGGAGGGAGGACTGAAAAACCCCTTC
TCAAAGCTGGTAATGCATACCACCAATTCAGAGTGAAGCCGAACTGCTGG
CCTAAGGTGCGTGGTGTGGCCATGAACCCGGTGGAGCATCCTCGTGGAGG
GAGGACCCCCAGCACATGGGCCATGCTAGCACTGTTAGGGGGGATCTTCC
CCCTGCCAGCAAGGTGGGTCTTATTGCACCCAGGAGGATTGGTCGCTTCG
AGGGTGACGGGTGGACTTGGGGCGGGGCGGCGCAAGGATAGTTCGTGGGG
GACGAGAGGCGGTCCTCACGGGATGCTGTCTTTACTTGTGGGGTTCGGCG
CACGGTGGAGAGGTGTTCCACTCCCACTCGGGA
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