EY684555

Overview
NameEY684555
Unique NameEY684555
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length995
Libraries
Library NameType
Sweet orange bark, greenhouse plantcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of EY684555 vs. ExPASy Swiss-Prot
Match: MDH_BURPP (Malate dehydrogenase OS=Burkholderia phytofirmans (strain DSM 17436 / PsJN) GN=mdh PE=3 SV=1)

HSP 1 Score: 98.2117 bits (243), Expect = 9.299e-26
Identity = 52/102 (50.98%), Postives = 64/102 (62.75%), Query Frame = 2 
Query:  278 FITTVQQRGAAIIKARKXXXXXXXXXXXCDHIRDWVLGTPEGTWVSMGEYSDGSYNVPPGHIYSFPKTCRNGEWTIVQGLSIDES*QKKLDLTAEELSEEKD 583
            FI TV +RGAAII+AR             DH+RDWVLGT  G WV+MG  SDGSY +P   IY  P TC NGE+  V+GL ID   ++K+D T  EL EE++
Sbjct:  220 FIPTVGKRGAAIIEARGLSSAASAANAAIDHVRDWVLGT-NGKWVTMGIPSDGSYGIPEDIIYGVPVTCENGEYKRVEGLEIDAFSREKMDGTLNELLEERE 320

HSP 2 Score: 40.4318 bits (93), Expect = 9.299e-26
Identity = 20/56 (35.71%), Postives = 31/56 (55.36%), Query Frame = 1 
Query:  130 PVSDVKNVIIWGNHSSSHYPDVNHATVNTAAGEKPVRELVKDDACVEWRIHNHSPT 297
            PV+ ++ + +WGNHS + YPD   AT    A  + + +L+ DD   EW  +   PT
Sbjct:  175 PVASIEKLAVWGNHSPTMYPDFRFAT----AEGQDLTKLINDD---EWNRNTFIPT 223
[Close]
BLAST of EY684555 vs. ExPASy Swiss-Prot
Match: MDH_RHOSR (Malate dehydrogenase OS=Rhodococcus sp. (strain RHA1) GN=mdh PE=3 SV=1)

HSP 1 Score: 94.3597 bits (233), Expect = 1.210e-25
Identity = 47/106 (44.34%), Postives = 61/106 (57.55%), Query Frame = 2 
Query:  266 LNGEFITTVQQRGAAIIKARKXXXXXXXXXXXCDHIRDWVLGTPEGTWVSMGEYSDGSYNVPPGHIYSFPKTCRNGEWTIVQGLSIDES*QKKLDLTAEELSEEKD 583
            L G+FI TV  RG+AII+AR             DH+ DWVLGTP+  W S+   S G+Y VP G + SFP    +G W IV GL ID+  +K +D +  EL  E+D
Sbjct:  218 LTGDFIPTVANRGSAIIEARGASSAASAANAAIDHVHDWVLGTPDDDWTSVALPSTGAYGVPEGLVSSFPVRSVDGSWQIVDGLEIDDFSRKLIDASVGELESERD 323

HSP 2 Score: 43.8986 bits (102), Expect = 1.210e-25
Identity = 17/31 (54.84%), Postives = 22/31 (70.97%), Query Frame = 1 
Query:  133 VSDVKNVIIWGNHSSSHYPDVNHATVNTAAG 225
            V D++ V IWGNHSS+ YPD+ HA V   +G
Sbjct:  178 VKDIRRVAIWGNHSSTQYPDIFHARVGDRSG 208
[Close]
BLAST of EY684555 vs. ExPASy Swiss-Prot
Match: MDH_XANC5 (Malate dehydrogenase OS=Xanthomonas campestris pv. vesicatoria (strain 85-10) GN=mdh PE=3 SV=1)

HSP 1 Score: 99.7525 bits (247), Expect = 1.210e-25
Identity = 53/101 (52.48%), Postives = 65/101 (64.36%), Query Frame = 2 
Query:  278 FITTVQQRGAAIIKARKXXXXXXXXXXXCDHIRDWVLGTPEGTWVSMGEYSDGSYNVPPGHIYSFPKTCRNGEWTIVQGLSIDES*QKKLDLTAEELSEEK 580
            FI TV +RGAAII+AR             DHIRDWVLGT  G WV+MG  SDGSY +P G ++ FP T  NG++TIV+ L ID+  QK +D T  EL EE+
Sbjct:  221 FIPTVGKRGAAIIEARGLSSAASAANAAIDHIRDWVLGT-NGKWVTMGVPSDGSYGIPEGVMFGFPVTTENGKYTIVKDLPIDDFSQKYIDKTLAELEEER 320

HSP 2 Score: 38.5058 bits (88), Expect = 1.210e-25
Identity = 19/56 (33.93%), Postives = 29/56 (51.79%), Query Frame = 1 
Query:  130 PVSDVKNVIIWGNHSSSHYPDVNHATVNTAAGEKPVRELVKDDACVEWRIHNHSPT 297
            PV+ ++ + +WGNHS + YPD   AT + A+    V + + D    EW      PT
Sbjct:  176 PVAGIEKLAVWGNHSPTMYPDYRFATADGAS----VGDAINDQ---EWNASTFIPT 224
[Close]
BLAST of EY684555 vs. ExPASy Swiss-Prot
Match: MDH_XANAC (Malate dehydrogenase OS=Xanthomonas axonopodis pv. citri GN=mdh PE=3 SV=1)

HSP 1 Score: 99.7525 bits (247), Expect = 1.575e-25
Identity = 53/101 (52.48%), Postives = 65/101 (64.36%), Query Frame = 2 
Query:  278 FITTVQQRGAAIIKARKXXXXXXXXXXXCDHIRDWVLGTPEGTWVSMGEYSDGSYNVPPGHIYSFPKTCRNGEWTIVQGLSIDES*QKKLDLTAEELSEEK 580
            FI TV +RGAAII+AR             DHIRDWVLGT  G WV+MG  SDGSY +P G ++ FP T  NG++TIV+ L ID+  QK +D T  EL EE+
Sbjct:  221 FIPTVGKRGAAIIEARGLSSAASAANAAIDHIRDWVLGT-SGKWVTMGVPSDGSYGIPEGVMFGFPVTTENGKYTIVKDLPIDDFSQKYIDKTLAELEEER 320

HSP 2 Score: 38.1206 bits (87), Expect = 1.575e-25
Identity = 18/56 (32.14%), Postives = 29/56 (51.79%), Query Frame = 1 
Query:  130 PVSDVKNVIIWGNHSSSHYPDVNHATVNTAAGEKPVRELVKDDACVEWRIHNHSPT 297
            PV+ ++ + +WGNHS + YPD   AT + A+    + + + D    EW      PT
Sbjct:  176 PVAGIEKLAVWGNHSPTMYPDYRFATADGAS----IGDAINDQ---EWNASTFIPT 224
[Close]
BLAST of EY684555 vs. ExPASy Swiss-Prot
Match: MDH_BURCJ (Malate dehydrogenase OS=Burkholderia cepacia (strain J2315 / LMG 16656) GN=mdh PE=3 SV=1)

HSP 1 Score: 99.3673 bits (246), Expect = 1.575e-25
Identity = 53/102 (51.96%), Postives = 64/102 (62.75%), Query Frame = 2 
Query:  278 FITTVQQRGAAIIKARKXXXXXXXXXXXCDHIRDWVLGTPEGTWVSMGEYSDGSYNVPPGHIYSFPKTCRNGEWTIVQGLSIDES*QKKLDLTAEELSEEKD 583
            FI TV +RGAAII+AR             DH+RDWVLGT  G WV+MG  SDGSY +P   IY  P TC NGE+  V+GL ID   ++K+D T  EL EE+D
Sbjct:  220 FIPTVGKRGAAIIEARGLSSAASAANAAIDHVRDWVLGT-NGKWVTMGIPSDGSYGIPEDIIYGVPVTCENGEYKRVEGLEIDAFSREKMDGTLAELLEERD 320

HSP 2 Score: 38.5058 bits (88), Expect = 1.575e-25
Identity = 21/56 (37.50%), Postives = 30/56 (53.57%), Query Frame = 1 
Query:  130 PVSDVKNVIIWGNHSSSHYPDVNHATVNTAAGEKPVRELVKDDACVEWRIHNHSPT 297
            PV+ ++ + +WGNHS + YPD   A   TA GE  + +L+ DD    W      PT
Sbjct:  175 PVASIEKLAVWGNHSPTMYPDFRFA---TAEGES-LLKLINDDV---WNRDTFIPT 223
[Close]
BLAST of EY684555 vs. ExPASy Swiss-Prot
Match: MDH_BURCH (Malate dehydrogenase OS=Burkholderia cenocepacia (strain HI2424) GN=mdh PE=3 SV=1)

HSP 1 Score: 99.3673 bits (246), Expect = 1.575e-25
Identity = 53/102 (51.96%), Postives = 64/102 (62.75%), Query Frame = 2 
Query:  278 FITTVQQRGAAIIKARKXXXXXXXXXXXCDHIRDWVLGTPEGTWVSMGEYSDGSYNVPPGHIYSFPKTCRNGEWTIVQGLSIDES*QKKLDLTAEELSEEKD 583
            FI TV +RGAAII+AR             DH+RDWVLGT  G WV+MG  SDGSY +P   IY  P TC NGE+  V+GL ID   ++K+D T  EL EE+D
Sbjct:  220 FIPTVGKRGAAIIEARGLSSAASAANAAIDHVRDWVLGT-NGKWVTMGIPSDGSYGIPEDIIYGVPVTCENGEYKRVEGLEIDAFSREKMDGTLAELLEERD 320

HSP 2 Score: 38.5058 bits (88), Expect = 1.575e-25
Identity = 21/56 (37.50%), Postives = 30/56 (53.57%), Query Frame = 1 
Query:  130 PVSDVKNVIIWGNHSSSHYPDVNHATVNTAAGEKPVRELVKDDACVEWRIHNHSPT 297
            PV+ ++ + +WGNHS + YPD   A   TA GE  + +L+ DD    W      PT
Sbjct:  175 PVASIEKLAVWGNHSPTMYPDFRFA---TAEGES-LLKLINDDV---WNRDTFIPT 223
[Close]
BLAST of EY684555 vs. ExPASy Swiss-Prot
Match: MDH_BURCC (Malate dehydrogenase OS=Burkholderia cenocepacia (strain MC0-3) GN=mdh PE=3 SV=1)

HSP 1 Score: 99.3673 bits (246), Expect = 1.575e-25
Identity = 53/102 (51.96%), Postives = 64/102 (62.75%), Query Frame = 2 
Query:  278 FITTVQQRGAAIIKARKXXXXXXXXXXXCDHIRDWVLGTPEGTWVSMGEYSDGSYNVPPGHIYSFPKTCRNGEWTIVQGLSIDES*QKKLDLTAEELSEEKD 583
            FI TV +RGAAII+AR             DH+RDWVLGT  G WV+MG  SDGSY +P   IY  P TC NGE+  V+GL ID   ++K+D T  EL EE+D
Sbjct:  220 FIPTVGKRGAAIIEARGLSSAASAANAAIDHVRDWVLGT-NGKWVTMGIPSDGSYGIPEDIIYGVPVTCENGEYKRVEGLEIDAFSREKMDGTLAELLEERD 320

HSP 2 Score: 38.5058 bits (88), Expect = 1.575e-25
Identity = 21/56 (37.50%), Postives = 30/56 (53.57%), Query Frame = 1 
Query:  130 PVSDVKNVIIWGNHSSSHYPDVNHATVNTAAGEKPVRELVKDDACVEWRIHNHSPT 297
            PV+ ++ + +WGNHS + YPD   A   TA GE  + +L+ DD    W      PT
Sbjct:  175 PVASIEKLAVWGNHSPTMYPDFRFA---TAEGES-LLKLINDDV---WNRDTFIPT 223
[Close]
BLAST of EY684555 vs. ExPASy Swiss-Prot
Match: MDH_BURCA (Malate dehydrogenase OS=Burkholderia cenocepacia (strain AU 1054) GN=mdh PE=3 SV=1)

HSP 1 Score: 99.3673 bits (246), Expect = 1.575e-25
Identity = 53/102 (51.96%), Postives = 64/102 (62.75%), Query Frame = 2 
Query:  278 FITTVQQRGAAIIKARKXXXXXXXXXXXCDHIRDWVLGTPEGTWVSMGEYSDGSYNVPPGHIYSFPKTCRNGEWTIVQGLSIDES*QKKLDLTAEELSEEKD 583
            FI TV +RGAAII+AR             DH+RDWVLGT  G WV+MG  SDGSY +P   IY  P TC NGE+  V+GL ID   ++K+D T  EL EE+D
Sbjct:  220 FIPTVGKRGAAIIEARGLSSAASAANAAIDHVRDWVLGT-NGKWVTMGIPSDGSYGIPEDIIYGVPVTCENGEYKRVEGLEIDAFSREKMDGTLAELLEERD 320

HSP 2 Score: 38.5058 bits (88), Expect = 1.575e-25
Identity = 21/56 (37.50%), Postives = 30/56 (53.57%), Query Frame = 1 
Query:  130 PVSDVKNVIIWGNHSSSHYPDVNHATVNTAAGEKPVRELVKDDACVEWRIHNHSPT 297
            PV+ ++ + +WGNHS + YPD   A   TA GE  + +L+ DD    W      PT
Sbjct:  175 PVASIEKLAVWGNHSPTMYPDFRFA---TAEGES-LLKLINDDV---WNRDTFIPT 223
[Close]
BLAST of EY684555 vs. ExPASy Swiss-Prot
Match: MDH_BURP8 (Malate dehydrogenase OS=Burkholderia phymatum (strain DSM 17167 / STM815) GN=mdh PE=3 SV=1)

HSP 1 Score: 96.6709 bits (239), Expect = 2.050e-25
Identity = 51/102 (50.00%), Postives = 63/102 (61.76%), Query Frame = 2 
Query:  278 FITTVQQRGAAIIKARKXXXXXXXXXXXCDHIRDWVLGTPEGTWVSMGEYSDGSYNVPPGHIYSFPKTCRNGEWTIVQGLSIDES*QKKLDLTAEELSEEKD 583
            FI TV +RGAAII+AR             DH+RDWVLGT  G WV+MG  SDGSY +P   +Y    TC NGE+  V+GL ID   ++K+D T  EL EE+D
Sbjct:  220 FIPTVGKRGAAIIEARGLSSAASAANAAIDHVRDWVLGT-NGKWVTMGIPSDGSYGIPEDIVYGVAVTCENGEYKRVEGLQIDAFSREKMDNTLNELLEERD 320

HSP 2 Score: 40.817 bits (94), Expect = 2.050e-25
Identity = 20/56 (35.71%), Postives = 32/56 (57.14%), Query Frame = 1 
Query:  130 PVSDVKNVIIWGNHSSSHYPDVNHATVNTAAGEKPVRELVKDDACVEWRIHNHSPT 297
            PVS ++ +++WGNHS + YPD   AT    A  + + +L+ D+   EW  +   PT
Sbjct:  175 PVSSIEKLVVWGNHSPTMYPDFRVAT----AEGQDLTKLINDE---EWNRNTFIPT 223
[Close]
BLAST of EY684555 vs. ExPASy Swiss-Prot
Match: MDH_AZOSB (Malate dehydrogenase OS=Azoarcus sp. (strain BH72) GN=mdh PE=3 SV=1)

HSP 1 Score: 102.064 bits (253), Expect = 3.473e-25
Identity = 53/102 (51.96%), Postives = 67/102 (65.69%), Query Frame = 2 
Query:  278 FITTVQQRGAAIIKARKXXXXXXXXXXXCDHIRDWVLGTPEGTWVSMGEYSDGSYNVPPGHIYSFPKTCRNGEWTIVQGLSIDES*QKKLDLTAEELSEEKD 583
            F+ TV +RGAAII+AR             DHIRDWVLG+  G WV+MG  SDGSY +P G IY FP T  NGE+ IVQGL IDE  ++++ +T  EL EE++
Sbjct:  223 FLPTVGKRGAAIIEARGLSSAASAANAAIDHIRDWVLGS-NGEWVTMGIPSDGSYGIPEGVIYGFPVTTENGEYKIVQGLEIDEFSRERMTVTLNELLEERE 323

HSP 2 Score: 34.6538 bits (78), Expect = 3.473e-25
Identity = 16/43 (37.21%), Postives = 25/43 (58.14%), Query Frame = 1 
Query:  133 VSDVKNVIIWGNHSSSHYPDVNHATVNTAAGEKPVRELVKDDA 261
            VS +KN+++WGNHS + Y D      N  +    V++L+ D A
Sbjct:  179 VSSLKNLVVWGNHSPTMYADYRFVKSNGDS----VKDLINDAA 217
The following BLAST results are available for this feature:
BLAST of EY684555 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 175
Match NameE-valueIdentityDescription
MDH_BURPP9.299e-2650.98Malate dehydrogenase OS=Burkholderia phytofirmans ... [more]
MDH_RHOSR1.210e-2544.34Malate dehydrogenase OS=Rhodococcus sp. (strain RH... [more]
MDH_XANC51.210e-2552.48Malate dehydrogenase OS=Xanthomonas campestris pv.... [more]
MDH_XANAC1.575e-2552.48Malate dehydrogenase OS=Xanthomonas axonopodis pv.... [more]
MDH_BURCJ1.575e-2551.96Malate dehydrogenase OS=Burkholderia cepacia (stra... [more]
MDH_BURCH1.575e-2551.96Malate dehydrogenase OS=Burkholderia cenocepacia (... [more]
MDH_BURCC1.575e-2551.96Malate dehydrogenase OS=Burkholderia cenocepacia (... [more]
MDH_BURCA1.575e-2551.96Malate dehydrogenase OS=Burkholderia cenocepacia (... [more]
MDH_BURP82.050e-2550.00Malate dehydrogenase OS=Burkholderia phymatum (str... [more]
MDH_AZOSB3.473e-2551.96Malate dehydrogenase OS=Azoarcus sp. (strain BH72)... [more]

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Properties
Property NameValue
Genbank descriptionCS00-C2-003-001-E07-CT.F Sweet orange bark, greenhouse plant Citrus sinensis cDNA, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>EY684555 ID=EY684555; Name=EY684555; organism=Citrus sinensis; type=EST; length=995bp
GGGGGGGGGTGAGAGGTGTGNTGTGTAAACGCTTTATATTATTTAACCTT
TGTGTATGTGGGTNAAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNT
GCTCAACCACGCGTCCGCGGACGCGTGTGCCCGTATCTGATGTTAAGAAT
GTGATTATTTGGGGAAATCATTCATCATCTCATTACCCTGATGTCAACCA
TGCAACTGTCAATACTGCAGCTGGGGAAAAGCCTGTCCGTGAGCTTGTCA
AGGATGATGCATGCGTTGAATGGAGAATTCATAACCACAGTCCAACAACG
TGGTGCTGCAATCATCAAAGCCAGAAAGCTCTCGAGTGCTCTGTCTGCTG
CTAGCTCTGCTTGTGACCATATCCGTGATTGGGTTCTTGGAACTCCTGAG
GGCACCTGGGTCTCCATGGGGGAATACTCTGATGGTTCATACAATGTACC
ACCTGGGCACATTTACTCATTCCCAAAAACCTGCCGCAACGGTGAATGGA
CAATAGTTCAAGGACTCAGCATTGATGAATCCTAACAGAAGAAATTGGAC
TTGACAGCAGAAGAACTCTCCGAGGAAAAAGACCTCACATACCCATGTCC
CCACTTAAATGCCTTCAACCCTTGCAGATTGTGAGTGACCAAGACCTCAA
ACTTAGTCTCAGAAGATAGCAAATATTACAGATTCACAAGAAAAGCCCTG
GTTGGAACTCGAGAGGAACGCATAGAGTAATGTGAAACACCTCCGCAACG
AAAGCTGGGGGCTCGCGGGCTGGAAATTATCGGGAATGGGACACGGGTAG
TAGCCCCCTCCAGGGAATAAGTGGAGATGCGATGATCACTGAACATAATC
TGCTATCCACCGCTCATCACCCCCTTTCCTATTTACAACACAACCAATAA
ACAAGCAAACAATTAATATATCATATAAATTGTCATAAAGAATAGTAAGA
CAAAATAAGATAAAAAATAAAAGGAATAAAAAACAGAGAGGTGTC
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