EY744419

Overview
NameEY744419
Unique NameEY744419
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length962
Libraries
Library NameType
Sweet orange flower, greenhouse plantcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of EY744419 vs. ExPASy Swiss-Prot
Match: CHIC_SECCE (Basic endochitinase C OS=Secale cereale GN=rscc PE=1 SV=1)

HSP 1 Score: 95.9005 bits (237), Expect = 1.047e-27
Identity = 47/107 (43.93%), Postives = 64/107 (59.81%), Query Frame = 2 
Query:  272 FLPNLAMKLLGGWTDAPDGEYAWGYCYITQVSPASSHYGHP--YWPCPLPGLYYGRGPIQLAWKCDYLVGGEALWVDLLNKPDLLATDPVLSSRSSIWLLDDCPSPQ 586
            FL   + +  GGW  APDG +AWGYC+  Q   A++ Y  P   WPC     YYGRGPIQL+   +Y   G A+ VDLL  PDL+ATDP +S ++++W      +P+
Sbjct:   83 FLAQTSHETTGGWATAPDGAFAWGYCF-KQERGAAADYCTPSAQWPCAPGKRYYGRGPIQLSHNYNYGPAGRAIGVDLLRNPDLVATDPTVSFKTALWFWMTAQAPK 188

HSP 2 Score: 42.743 bits (99), Expect = 1.047e-27
Identity = 19/40 (47.50%), Postives = 23/40 (57.50%), Query Frame = 1 
Query:  160 TDGGELGKIVSREMFEHLLPDTNDARCAATGFYTYDAFFA 279
            T  G +  I+S   F+ +L   ND  C A GFYTYDAF A
Sbjct:   20 TAHGSVSSIISHAQFDRMLLHRNDGACQAKGFYTYDAFVA 59

HSP 3 Score: 26.9498 bits (58), Expect = 1.047e-27
Identity = 10/20 (50.00%), Postives = 11/20 (55.00%), Query Frame = 3 
Query:  561 YWMTAHPHKPSCHQVSIDSW 620
            +WMTA   KPS H V    W
Sbjct:  180 FWMTAQAPKPSSHAVITGKW 199
[Close]
BLAST of EY744419 vs. ExPASy Swiss-Prot
Match: CHI2_SOLTU (Endochitinase 2 (Fragment) OS=Solanum tuberosum GN=CHTB2 PE=2 SV=1)

HSP 1 Score: 96.2857 bits (238), Expect = 1.752e-27
Identity = 47/106 (44.34%), Postives = 62/106 (58.49%), Query Frame = 2 
Query:  272 FLPNLAMKLLGGWTDAPDGEYAWGYCYIT-QVSPASSHYGHPYWPCPLPGLYYGRGPIQLAWKCDYLVGGEALWVDLLNKPDLLATDPVLSSRSSIWLLDDCPSPQ 586
            F    + +  GGW  APDG YAWGYC++  Q SP         WPC     Y+GRGPIQ++   +Y   G A+ VDLLN PDL+ATD ++S +S+IW      SP+
Sbjct:  125 FFAQTSHETTGGWASAPDGPYAWGYCFLREQGSPGDYCTPSNQWPCAPGRKYFGRGPIQISHNYNYGPCGRAIGVDLLNNPDLVATDSIISFKSAIWFWMTPQSPK 230

HSP 2 Score: 39.2762 bits (90), Expect = 1.752e-27
Identity = 17/41 (41.46%), Postives = 26/41 (63.41%), Query Frame = 1 
Query:  169 GELGKIVSREMFEHLLPDTNDARCAATG-FYTYDAFFAQSG 288
            G+LG ++S  MF+ +L   ND  C   G FY+Y+AF + +G
Sbjct:   64 GDLGGVISNSMFDQMLNHRNDNACQGKGNFYSYNAFISAAG 104

HSP 3 Score: 29.261 bits (64), Expect = 1.752e-27
Identity = 10/21 (47.62%), Postives = 12/21 (57.14%), Query Frame = 3 
Query:  561 YWMTAHPHKPSCHQVSIDSWK 623
            +WMT    KPSCH V    W+
Sbjct:  222 FWMTPQSPKPSCHDVITGRWQ 242
[Close]
BLAST of EY744419 vs. ExPASy Swiss-Prot
Match: CHI12_ORYSJ (Chitinase 12 OS=Oryza sativa subsp. japonica GN=Cht12 PE=2 SV=2)

HSP 1 Score: 87.8113 bits (216), Expect = 6.448e-27
Identity = 45/111 (40.54%), Postives = 61/111 (54.95%), Query Frame = 2 
Query:  272 FLPNLAMKLLGGWTDAPDGEYAWGYCYITQVSPASSHYGHPY------WPCPLPGLYYGRGPIQLAWKCDYLVGGEALWVDLLNKPDLLATDPVLSSRSSIWLLDDCPSPQ 586
            FL   + +  GGW  APDG YAWGYC+  +   A+   G  Y      WPC     YYGRGPIQL++  +Y   G+A+  DLL  PDL+A+D  +S  ++ W      SP+
Sbjct:  140 FLAQTSHETTGGWATAPDGPYAWGYCFKEENGGAA---GPDYCQQSAQWPCAAGKKYYGRGPIQLSYNFNYGPAGQAIGADLLGDPDLVASDATVSFDTAFWFWMTPQSPK 247

HSP 2 Score: 45.0542 bits (105), Expect = 6.448e-27
Identity = 20/38 (52.63%), Postives = 25/38 (65.79%), Query Frame = 1 
Query:  166 GGELGKIVSREMFEHLLPDTNDARCAATGFYTYDAFFA 279
            G  +  IVSR +F+ +L   NDA C A+ FYTYDAF A
Sbjct:   78 GSGVASIVSRSLFDQMLLHRNDAACPASNFYTYDAFVA 115

HSP 3 Score: 30.0314 bits (66), Expect = 6.448e-27
Identity = 10/20 (50.00%), Postives = 12/20 (60.00%), Query Frame = 3 
Query:  561 YWMTAHPHKPSCHQVSIDSW 620
            +WMT    KPSCH V+   W
Sbjct:  239 FWMTPQSPKPSCHAVATGQW 258
[Close]
BLAST of EY744419 vs. ExPASy Swiss-Prot
Match: CHID_SOLLC (Basic endochitinase (Fragment) OS=Solanum lycopersicum GN=CHI14 PE=2 SV=1)

HSP 1 Score: 94.3597 bits (233), Expect = 1.099e-26
Identity = 44/106 (41.51%), Postives = 61/106 (57.55%), Query Frame = 2 
Query:  272 FLPNLAMKLLGGWTDAPDGEYAWGYCYIT-QVSPASSHYGHPYWPCPLPGLYYGRGPIQLAWKCDYLVGGEALWVDLLNKPDLLATDPVLSSRSSIWLLDDCPSPQ 586
            FL   + +  GGW  APDG Y+WGYCY   Q SP         WPC     Y+GRGPIQ+++  +Y   G A+ V+LLN PDL+A D V+S ++++W       P+
Sbjct:   63 FLAQTSHETTGGWATAPDGPYSWGYCYNREQGSPGDYCASSQQWPCAPGKKYFGRGPIQISYNYNYGAAGSAIGVNLLNNPDLVANDAVVSFKTALWFWMTAQQPK 168

HSP 2 Score: 40.4318 bits (93), Expect = 1.099e-26
Identity = 15/33 (45.45%), Postives = 22/33 (66.67%), Query Frame = 1 
Query:  175 LGKIVSREMFEHLLPDTNDARCAATGFYTYDAF 273
            +  ++S+ +FE +L   ND  C A GFYTY+AF
Sbjct:    5 ISSLISKNLFERILVHRNDRACGAKGFYTYEAF 37

HSP 3 Score: 27.335 bits (59), Expect = 1.099e-26
Identity = 10/20 (50.00%), Postives = 11/20 (55.00%), Query Frame = 3 
Query:  561 YWMTAHPHKPSCHQVSIDSW 620
            +WMTA   KPS H V    W
Sbjct:  160 FWMTAQQPKPSAHDVITGRW 179
[Close]
BLAST of EY744419 vs. ExPASy Swiss-Prot
Match: CHI6_POPTR (Acidic endochitinase WIN6 OS=Populus trichocarpa GN=WIN6 PE=2 SV=2)

HSP 1 Score: 87.8113 bits (216), Expect = 1.407e-26
Identity = 42/105 (40.00%), Postives = 62/105 (59.05%), Query Frame = 2 
Query:  272 FLPNLAMKLLGGWTDAPDGEYAWGYCYITQVSPASSHYGHPYWPCPLPGLYYGRGPIQLAWKCDYLVGGEALWVDLLNKPDLLATDPVLSSRSSIWLLDDCPSPQ 586
            FL   + +  GGW DAP G YAWGYCY+ +++          + C     Y GRGPIQL+W  +Y + G+ L + LL +P+L+ TDPV+S +++IW      SP+
Sbjct:  147 FLGQTSHETTGGWPDAPCGPYAWGYCYLKEINCQPYCDPSSNYQCVAGKQYCGRGPIQLSWNYNYGLCGDDLKLPLLQEPELVETDPVISFKTAIWFWMKPQSPK 251

HSP 2 Score: 46.9802 bits (110), Expect = 1.407e-26
Identity = 23/48 (47.92%), Postives = 28/48 (58.33%), Query Frame = 1 
Query:  136 GERDTGHRTDGGELGKIVSREMFEHLLPDTNDARCAATGFYTYDAFFA 279
            G  D G   D G L  I+ +  F+ LL   NDARC A GFYTY+AF +
Sbjct:   76 GGDDGGGDGDDGYLSDIIPKSKFDALLKFRNDARCPAAGFYTYNAFIS 123

HSP 3 Score: 26.9498 bits (58), Expect = 1.407e-26
Identity = 9/20 (45.00%), Postives = 11/20 (55.00%), Query Frame = 3 
Query:  561 YWMTAHPHKPSCHQVSIDSW 620
            +WM     KPSCH V   +W
Sbjct:  243 FWMKPQSPKPSCHAVITGNW 262
[Close]
BLAST of EY744419 vs. ExPASy Swiss-Prot
Match: CHI1_ORYSJ (Chitinase 1 OS=Oryza sativa subsp. japonica GN=Cht1 PE=2 SV=1)

HSP 1 Score: 85.5001 bits (210), Expect = 8.744e-26
Identity = 41/111 (36.94%), Postives = 63/111 (56.76%), Query Frame = 2 
Query:  272 FLPNLAMKLLGGWTDAPDGEYAWGYCYITQVSPASSHYGHPY------WPCPLPGLYYGRGPIQLAWKCDYLVGGEALWVDLLNKPDLLATDPVLSSRSSIWLLDDCPSPQ 586
            FL   + +  GGW  APDG Y+WGYC+  +    + + G  Y      WPC     YYGRGPIQ+++  +Y   G+A+  +LL+ PDL+A+D  +S +++ W      SP+
Sbjct:  137 FLAQTSHETTGGWATAPDGPYSWGYCFKEE---NNGNVGSDYCVQSSQWPCAAGKKYYGRGPIQISYNYNYGPAGQAIGSNLLSNPDLVASDATVSFKTAFWFWMTPQSPK 244

HSP 2 Score: 44.2838 bits (103), Expect = 8.744e-26
Identity = 20/38 (52.63%), Postives = 24/38 (63.16%), Query Frame = 1 
Query:  166 GGELGKIVSREMFEHLLPDTNDARCAATGFYTYDAFFA 279
            G  +  IVSR +F+ +L   NDA C A  FYTYDAF A
Sbjct:   76 GSGVASIVSRSLFDQMLLHRNDAACPAKNFYTYDAFVA 113

HSP 3 Score: 29.261 bits (64), Expect = 8.744e-26
Identity = 10/20 (50.00%), Postives = 11/20 (55.00%), Query Frame = 3 
Query:  561 YWMTAHPHKPSCHQVSIDSW 620
            +WMT    KPSCH V    W
Sbjct:  236 FWMTPQSPKPSCHAVMTGQW 255
[Close]
BLAST of EY744419 vs. ExPASy Swiss-Prot
Match: CHIT_PHAVU (Endochitinase OS=Phaseolus vulgaris PE=1 SV=1)

HSP 1 Score: 109.768 bits (273), Expect = 3.132e-25
Identity = 49/105 (46.67%), Postives = 68/105 (64.76%), Query Frame = 2 
Query:  272 FLPNLAMKLLGGWTDAPDGEYAWGYCYITQVSPASSHYGHPYWPCPLPGLYYGRGPIQLAWKCDYLVGGEALWVDLLNKPDLLATDPVLSSRSSIWLLDDCPSPQ 586
            FL   + +  GGW  APDG YAWGYC++ + +P++     P +PC     YYGRGPIQ++W  +Y   G A+ VDLLNKPDL+ATD V+S +S++W      SP+
Sbjct:  134 FLGQTSHETTGGWATAPDGPYAWGYCFVRERNPSTYCSATPQFPCAPGQQYYGRGPIQISWNYNYGQCGRAIGVDLLNKPDLVATDSVISFKSALWFWMTAQSPK 238

HSP 2 Score: 27.335 bits (59), Expect = 3.132e-25
Identity = 10/20 (50.00%), Postives = 11/20 (55.00%), Query Frame = 3 
Query:  561 YWMTAHPHKPSCHQVSIDSW 620
            +WMTA   KPS H V    W
Sbjct:  230 FWMTAQSPKPSSHDVITSRW 249
[Close]
BLAST of EY744419 vs. ExPASy Swiss-Prot
Match: CHI5_PHAVU (Endochitinase CH5B OS=Phaseolus vulgaris PE=3 SV=1)

HSP 1 Score: 109.768 bits (273), Expect = 3.133e-25
Identity = 49/105 (46.67%), Postives = 68/105 (64.76%), Query Frame = 2 
Query:  272 FLPNLAMKLLGGWTDAPDGEYAWGYCYITQVSPASSHYGHPYWPCPLPGLYYGRGPIQLAWKCDYLVGGEALWVDLLNKPDLLATDPVLSSRSSIWLLDDCPSPQ 586
            FL   + +  GGW  APDG YAWGYC++ + +P++     P +PC     YYGRGPIQ++W  +Y   G A+ VDLLNKPDL+ATD V+S +S++W      SP+
Sbjct:  133 FLGQTSHETTGGWATAPDGPYAWGYCFVRERNPSAYCSATPQFPCAPGQQYYGRGPIQISWNYNYGQCGRAIGVDLLNKPDLVATDSVISFKSALWFWMTAQSPK 237

HSP 2 Score: 27.335 bits (59), Expect = 3.133e-25
Identity = 10/20 (50.00%), Postives = 11/20 (55.00%), Query Frame = 3 
Query:  561 YWMTAHPHKPSCHQVSIDSW 620
            +WMTA   KPS H V    W
Sbjct:  229 FWMTAQSPKPSSHDVITSRW 248
[Close]
BLAST of EY744419 vs. ExPASy Swiss-Prot
Match: CHIB_ARATH (Basic endochitinase B OS=Arabidopsis thaliana GN=CHI-B PE=1 SV=3)

HSP 1 Score: 100.138 bits (248), Expect = 1.169e-24
Identity = 46/98 (46.94%), Postives = 63/98 (64.29%), Query Frame = 2 
Query:  272 FLPNLAMKLLGGWTDAPDGEYAWGYCYITQVSPASSHYGHPY--WPCPLPGLYYGRGPIQLAWKCDYLVGGEALWVDLLNKPDLLATDPVLSSRSSIW 559
            F    + +  GGW  APDG Y+WGYC+  + +PAS  Y  P   WPC     YYGRGP+QL+W  +Y + G A+ VDLLN PDL+A D V++ +++IW
Sbjct:  144 FFGQTSHETTGGWATAPDGPYSWGYCFKQEQNPASD-YCEPSATWPCASGKRYYGRGPMQLSWNYNYGLCGRAIGVDLLNNPDLVANDAVIAFKAAIW 240

HSP 2 Score: 35.039 bits (79), Expect = 1.169e-24
Identity = 19/48 (39.58%), Postives = 24/48 (50.00%), Query Frame = 3 
Query:  561 YWMTAHPHKPSCHQVSIDSWKTLLPTEPTCRAGFL-NYSLNQEFDQGG 701
            +WMTA P KPSCH V    W+   P++    AG L  Y +      GG
Sbjct:  241 FWMTAQPPKPSCHAVIAGQWQ---PSDADRAAGRLPGYGVITNIINGG 285
[Close]
BLAST of EY744419 vs. ExPASy Swiss-Prot
Match: CHI10_ORYSJ (Chitinase 10 OS=Oryza sativa subsp. japonica GN=Cht10 PE=2 SV=1)

HSP 1 Score: 85.8853 bits (211), Expect = 2.698e-23
Identity = 38/98 (38.78%), Postives = 59/98 (60.20%), Query Frame = 2 
Query:  272 FLPNLAMKLLGGWTDAPDGEYAWGYCYITQVSPASSHYG--HPYWPCPLPGLYYGRGPIQLAWKCDYLVGGEALWVDLLNKPDLLATDPVLSSRSSIW 559
            FL  ++ +  GGW  APDG YAWG CY  +++P SS+       WPC     Y+GRGPIQ++W  +Y   G+AL  D L  P+++A    ++ ++++W
Sbjct:  107 FLAQISHETTGGWATAPDGPYAWGLCYKEEINPQSSYCDATDKQWPCYPGKSYHGRGPIQISWNFNYGPAGQALGFDGLRNPEIVANCSDIAFQTALW 204

HSP 2 Score: 34.2686 bits (77), Expect = 2.698e-23
Identity = 15/41 (36.59%), Postives = 22/41 (53.66%), Query Frame = 1 
Query:  151 GHRTDGGELGKIVSREMFEHLLPDTNDARCAATGFYTYDAF 273
            G+      +  IVS +++  L    +DA C A GFYTY +F
Sbjct:   41 GYSPSPSPVSSIVSEQLYASLFLHKDDAACPARGFYTYASF 81

HSP 3 Score: 30.4166 bits (67), Expect = 2.698e-23
Identity = 10/21 (47.62%), Postives = 14/21 (66.67%), Query Frame = 3 
Query:  561 YWMTAHPHKPSCHQVSIDSWK 623
            +WMT    KPSCHQV +  ++
Sbjct:  205 FWMTPRDTKPSCHQVMVGEYR 225
The following BLAST results are available for this feature:
BLAST of EY744419 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 45
Match NameE-valueIdentityDescription
CHIC_SECCE1.047e-2743.93Basic endochitinase C OS=Secale cereale GN=rscc PE... [more]
CHI2_SOLTU1.752e-2744.34Endochitinase 2 (Fragment) OS=Solanum tuberosum GN... [more]
CHI12_ORYSJ6.448e-2740.54Chitinase 12 OS=Oryza sativa subsp. japonica GN=Ch... [more]
CHID_SOLLC1.099e-2641.51Basic endochitinase (Fragment) OS=Solanum lycopers... [more]
CHI6_POPTR1.407e-2640.00Acidic endochitinase WIN6 OS=Populus trichocarpa G... [more]
CHI1_ORYSJ8.744e-2636.94Chitinase 1 OS=Oryza sativa subsp. japonica GN=Cht... [more]
CHIT_PHAVU3.132e-2546.67Endochitinase OS=Phaseolus vulgaris PE=1 SV=1[more]
CHI5_PHAVU3.133e-2546.67Endochitinase CH5B OS=Phaseolus vulgaris PE=3 SV=1[more]
CHIB_ARATH1.169e-2446.94Basic endochitinase B OS=Arabidopsis thaliana GN=C... [more]
CHI10_ORYSJ2.698e-2338.78Chitinase 10 OS=Oryza sativa subsp. japonica GN=Ch... [more]

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Properties
Property NameValue
Genbank descriptionCS00-C5-003-008-D08-CT.F Sweet orange flower, greenhouse plant Citrus sinensis cDNA, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>EY744419 ID=EY744419; Name=EY744419; organism=Citrus sinensis; type=EST; length=962bp
CCACGCGTCCGGATAATTGATGTCGTACCCCTTGCTTTCAATGTGAGGCT
CATTGGGTCCTTACTAATTCTCTCTCTAGATTTATCTTTTGTACTAAGAG
GTTCAGCACAGAATTGAGGAAGCGGCGTTGTGTGCGGGGAACGTGATACT
GGCCACAGGACGGATGGCGGTGAACTGGGCAAGATCGTCTCACGGGAAAT
GTTTGAACACTTGCTTCCGGATACAAATGATGCCCGATGCGCTGCCACAG
GCTTCTACACTTATGATGCTTTTTTTGCCCAATCTGGCCATGAAACTACT
TGGGGGATGGACCGATGCACCTGATGGAGAATATGCTTGGGGATATTGCT
ATATTACGCAAGTGAGCCCTGCGTCGTCCCACTACGGTCATCCTTACTGG
CCATGTCCCTTGCCAGGGCTATACTATGGCCGAGGTCCCATTCAACTCGC
TTGGAAGTGCGATTACTTAGTGGGCGGAGAAGCCTTGTGGGTGGACTTGT
TGAACAAACCAGACCTACTTGCCACTGATCCTGTACTATCATCTCGGTCA
TCCATCTGGTTACTGGATGACTGCCCATCCCCACAAGCCATCCTGCCACC
AAGTCTCTATTGACTCATGGAAAACCCTTCTCCCAACTGAACCAACCTGC
CGAGCCGGCTTCCTGAATTACAGTCTTAACCAGGAATTTGATCAAGGTGG
GGGATTCCTCTGGGTCGAACTCTGGAGAATGCACCCCACCTCCCACAATG
GGTGGGGCAACTTTCCCCTGTTGGGGCGCCGAGGCCTCGGAACTAGACCC
CGGTGGAATAGCTCCTAGACACTTGGCGGAGACCATAAGATGAGCGCCCG
CTGCACCAAGCCCCTACCAATTCCTCCAGGACAAGACCGCTCCCCCGCGC
CTGCCACTCCCCTTACCCCACCGTCAACAGAACACCCTCCTGCTTCCTCT
ACCACGCACATG
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