EY744419

Overview
NameEY744419
Unique NameEY744419
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length962
Libraries
Library NameType
Sweet orange flower, greenhouse plantcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of EY744419 vs. ExPASy Swiss-Prot
Match: CHI2_BRANA (Endochitinase CH25 OS=Brassica napus PE=2 SV=1)

HSP 1 Score: 99.3673 bits (246), Expect = 3.591e-23
Identity = 47/107 (43.93%), Postives = 66/107 (61.68%), Query Frame = 2 
Query:  272 FLPNLAMKLLGGWTDAPDGEYAWGYCYITQVSPASSHYGHPY--WPCPLPGLYYGRGPIQLAWKCDYLVGGEALWVDLLNKPDLLATDPVLSSRSSIWLLDDCPSPQ 586
            F    + +  GGW  APDG Y+WGYC+  + +P SS+Y  P   WPC     YYGRGP+QL+W  +Y   G A+  DLLN PDL++ DPV++ +++IW      SP+
Sbjct:  129 FFGQTSHETTGGWATAPDGPYSWGYCFKQEQNP-SSNYCSPSAEWPCASGKSYYGRGPMQLSWNYNYGQCGRAIGSDLLNNPDLVSNDPVIAFKAAIWFWMTPQSPK 234

HSP 2 Score: 30.8018 bits (68), Expect = 3.591e-23
Identity = 10/21 (47.62%), Postives = 13/21 (61.90%), Query Frame = 3 
Query:  561 YWMTAHPHKPSCHQVSIDSWK 623
            +WMT    KPSCH V +  W+
Sbjct:  226 FWMTPQSPKPSCHAVIVGQWQ 246
[Close]
BLAST of EY744419 vs. ExPASy Swiss-Prot
Match: CHI4_SOLTU (Endochitinase 4 (Fragment) OS=Solanum tuberosum GN=CHTB4 PE=2 SV=1)

HSP 1 Score: 98.9821 bits (245), Expect = 3.596e-23
Identity = 49/106 (46.23%), Postives = 63/106 (59.43%), Query Frame = 2 
Query:  272 FLPNLAMKLLGGWTDAPDGEYAWGYCYIT-QVSPASSHYGHPYWPCPLPGLYYGRGPIQLAWKCDYLVGGEALWVDLLNKPDLLATDPVLSSRSSIWLLDDCPSPQ 586
            F    + +  GGW  APDG YAWGYC++  Q SP         WPC     Y+GRGPIQ++   +Y   G A+ VDLLN PDL+ATDPV+S +S+IW      SP+
Sbjct:  137 FFAQTSHETTGGWPTAPDGPYAWGYCFLREQGSPGDYCTPSGQWPCAPGRKYFGRGPIQISHNYNYGPCGRAIGVDLLNNPDLVATDPVISFKSAIWFWMTPQSPK 242

HSP 2 Score: 31.187 bits (69), Expect = 3.596e-23
Identity = 11/21 (52.38%), Postives = 13/21 (61.90%), Query Frame = 3 
Query:  561 YWMTAHPHKPSCHQVSIDSWK 623
            +WMT    KPSCH V I  W+
Sbjct:  234 FWMTPQSPKPSCHDVIIGRWQ 254
[Close]
BLAST of EY744419 vs. ExPASy Swiss-Prot
Match: CHI1_TOBAC (Endochitinase A OS=Nicotiana tabacum GN=CHN48 PE=1 SV=2)

HSP 1 Score: 98.2117 bits (243), Expect = 6.077e-23
Identity = 48/106 (45.28%), Postives = 63/106 (59.43%), Query Frame = 2 
Query:  272 FLPNLAMKLLGGWTDAPDGEYAWGYCYIT-QVSPASSHYGHPYWPCPLPGLYYGRGPIQLAWKCDYLVGGEALWVDLLNKPDLLATDPVLSSRSSIWLLDDCPSPQ 586
            F    + +  GGW  APDG YAWGYC++  Q SP         WPC     Y+GRGPIQ++   +Y   G A+ VDLLN PDL+ATDPV+S +S++W      SP+
Sbjct:  138 FFAQTSHETTGGWATAPDGPYAWGYCWLREQGSPGDYCTPSGQWPCAPGRKYFGRGPIQISHNYNYGPCGRAIGVDLLNNPDLVATDPVISFKSALWFWMTPQSPK 243

HSP 2 Score: 31.187 bits (69), Expect = 6.077e-23
Identity = 11/21 (52.38%), Postives = 13/21 (61.90%), Query Frame = 3 
Query:  561 YWMTAHPHKPSCHQVSIDSWK 623
            +WMT    KPSCH V I  W+
Sbjct:  235 FWMTPQSPKPSCHDVIIGRWQ 255
[Close]
BLAST of EY744419 vs. ExPASy Swiss-Prot
Match: CHI2_TOBAC (Endochitinase B OS=Nicotiana tabacum GN=CHN50 PE=1 SV=1)

HSP 1 Score: 98.2117 bits (243), Expect = 6.080e-23
Identity = 48/106 (45.28%), Postives = 63/106 (59.43%), Query Frame = 2 
Query:  272 FLPNLAMKLLGGWTDAPDGEYAWGYCYIT-QVSPASSHYGHPYWPCPLPGLYYGRGPIQLAWKCDYLVGGEALWVDLLNKPDLLATDPVLSSRSSIWLLDDCPSPQ 586
            F    + +  GGW  APDG YAWGYC++  Q SP         WPC     Y+GRGPIQ++   +Y   G A+ VDLLN PDL+ATDPV+S +S++W      SP+
Sbjct:  133 FFAQTSHETTGGWATAPDGPYAWGYCWLREQGSPGDYCTPSGQWPCAPGRKYFGRGPIQISHNYNYGPCGRAIGVDLLNNPDLVATDPVISFKSALWFWMTPQSPK 238

HSP 2 Score: 31.187 bits (69), Expect = 6.080e-23
Identity = 11/21 (52.38%), Postives = 13/21 (61.90%), Query Frame = 3 
Query:  561 YWMTAHPHKPSCHQVSIDSWK 623
            +WMT    KPSCH V I  W+
Sbjct:  230 FWMTPQSPKPSCHDVIIGRWQ 250
[Close]
BLAST of EY744419 vs. ExPASy Swiss-Prot
Match: CHI3_TOBAC (Endochitinase 3 OS=Nicotiana tabacum GN=CHN14 PE=3 SV=1)

HSP 1 Score: 96.2857 bits (238), Expect = 2.947e-22
Identity = 47/106 (44.34%), Postives = 63/106 (59.43%), Query Frame = 2 
Query:  272 FLPNLAMKLLGGWTDAPDGEYAWGYCYIT-QVSPASSHYGHPYWPCPLPGLYYGRGPIQLAWKCDYLVGGEALWVDLLNKPDLLATDPVLSSRSSIWLLDDCPSPQ 586
            F    + +  GGW  APDG YAWGYCY+  Q +P S       WPC     YYGRGPIQ+++  +Y   G A+  +LLN PDL+AT+ V+S +S+IW      SP+
Sbjct:  143 FFAQTSHETTGGWDTAPDGRYAWGYCYLREQGNPPSYCVQSSQWPCAPGQKYYGRGPIQISYNYNYGPCGRAIGQNLLNNPDLVATNAVVSFKSAIWFWMTAQSPK 248

HSP 2 Score: 30.8018 bits (68), Expect = 2.947e-22
Identity = 11/20 (55.00%), Postives = 12/20 (60.00%), Query Frame = 3 
Query:  561 YWMTAHPHKPSCHQVSIDSW 620
            +WMTA   KPSCH V    W
Sbjct:  240 FWMTAQSPKPSCHDVITGRW 259
[Close]
BLAST of EY744419 vs. ExPASy Swiss-Prot
Match: CHI9_ORYSJ (Chitinase 9 OS=Oryza sativa subsp. japonica GN=Cht9 PE=2 SV=1)

HSP 1 Score: 97.0561 bits (240), Expect = 4.989e-22
Identity = 44/106 (41.51%), Postives = 66/106 (62.26%), Query Frame = 2 
Query:  272 FLPNLAMKLLGGWTDAPDGEYAWGYCYITQVSPASSH-YGHPYWPCPLPGLYYGRGPIQLAWKCDYLVGGEALWVDLLNKPDLLATDPVLSSRSSIWLLDDCPSPQ 586
            FL   + +  GGW  APDG Y+WGYC+  ++   +S+      WPC     Y+GRGPIQL++  +Y   GEA+  DLLN P+L+A+DPV+S ++++W      SP+
Sbjct:  144 FLGQTSHETTGGWATAPDGPYSWGYCFKEEIGATASYCVPSAEWPCAPDKKYFGRGPIQLSYNYNYGPAGEAIGEDLLNNPELVASDPVVSFKTALWFWMTPQSPK 249

HSP 2 Score: 29.261 bits (64), Expect = 4.989e-22
Identity = 10/20 (50.00%), Postives = 11/20 (55.00%), Query Frame = 3 
Query:  561 YWMTAHPHKPSCHQVSIDSW 620
            +WMT    KPSCH V    W
Sbjct:  241 FWMTPQSPKPSCHDVITGQW 260
[Close]
BLAST of EY744419 vs. ExPASy Swiss-Prot
Match: CHIT_SOLTU (Endochitinase OS=Solanum tuberosum PE=2 SV=1)

HSP 1 Score: 95.5153 bits (236), Expect = 4.991e-22
Identity = 45/106 (42.45%), Postives = 63/106 (59.43%), Query Frame = 2 
Query:  272 FLPNLAMKLLGGWTDAPDGEYAWGYCYITQV-SPASSHYGHPYWPCPLPGLYYGRGPIQLAWKCDYLVGGEALWVDLLNKPDLLATDPVLSSRSSIWLLDDCPSPQ 586
            F    + +  GGW  APDG YAWGYC++ +  +P         WPC     Y+GRGPIQ++   +Y   G A+ VDLLN PDL+ATDPV+S ++++W      SP+
Sbjct:  137 FFAQTSHETTGGWASAPDGPYAWGYCFLRERGNPGDYCPPSSQWPCAPGRKYFGRGPIQISHNYNYGPCGRAIGVDLLNNPDLVATDPVISFKTALWFWMTPQSPK 242

HSP 2 Score: 30.8018 bits (68), Expect = 4.991e-22
Identity = 11/20 (55.00%), Postives = 12/20 (60.00%), Query Frame = 3 
Query:  561 YWMTAHPHKPSCHQVSIDSW 620
            +WMT    KPSCH V I  W
Sbjct:  234 FWMTPQSPKPSCHDVIIGRW 253
[Close]
BLAST of EY744419 vs. ExPASy Swiss-Prot
Match: CHI1_HORVU (26 kDa endochitinase 1 OS=Hordeum vulgare PE=2 SV=4)

HSP 1 Score: 97.4413 bits (241), Expect = 1.862e-21
Identity = 48/107 (44.86%), Postives = 65/107 (60.75%), Query Frame = 2 
Query:  272 FLPNLAMKLLGGWTDAPDGEYAWGYCYITQVSPASSHYGHP--YWPCPLPGLYYGRGPIQLAWKCDYLVGGEALWVDLLNKPDLLATDPVLSSRSSIWLLDDCPSPQ 586
            FL   + +  GGW  APDG +AWGYC+  Q   A+S+Y  P   WPC     YYGRGPIQL+   +Y   G A+ VDLL  PDL+ATDP +S ++++W      +P+
Sbjct:  135 FLAQTSHETTGGWATAPDGAFAWGYCF-KQERGATSNYCTPSAQWPCAPGKSYYGRGPIQLSHNYNYGPAGRAIGVDLLRNPDLVATDPTVSFKTAMWFWMTAQAPK 240

HSP 2 Score: 26.9498 bits (58), Expect = 1.862e-21
Identity = 10/20 (50.00%), Postives = 11/20 (55.00%), Query Frame = 3 
Query:  561 YWMTAHPHKPSCHQVSIDSW 620
            +WMTA   KPS H V    W
Sbjct:  232 FWMTAQAPKPSSHAVITGQW 251
[Close]
BLAST of EY744419 vs. ExPASy Swiss-Prot
Match: CHI11_ORYSJ (Chitinase 11 OS=Oryza sativa subsp. japonica GN=Cht11 PE=2 SV=1)

HSP 1 Score: 92.4337 bits (228), Expect = 1.872e-21
Identity = 42/105 (40.00%), Postives = 63/105 (60.00%), Query Frame = 2 
Query:  272 FLPNLAMKLLGGWTDAPDGEYAWGYCYITQVSPASSHYGHPYWPCPLPGLYYGRGPIQLAWKCDYLVGGEALWVDLLNKPDLLATDPVLSSRSSIWLLDDCPSPQ 586
            F    + +  GGW  APDG +AWGYC + +++P+   Y             YGRGPIQL  K +Y + G+AL +DL+N PDL+++DPV++ R++IW      SP+
Sbjct:   84 FFGQTSHETTGGWATAPDGPFAWGYCRVNEITPSDPPY-------------YGRGPIQLTHKYNYQLAGDALGLDLVNNPDLVSSDPVVAFRTAIWFWMTAQSPK 175

HSP 2 Score: 31.9574 bits (71), Expect = 1.872e-21
Identity = 11/20 (55.00%), Postives = 13/20 (65.00%), Query Frame = 3 
Query:  561 YWMTAHPHKPSCHQVSIDSW 620
            +WMTA   KPSCH V  + W
Sbjct:  167 FWMTAQSPKPSCHDVITNQW 186
[Close]
BLAST of EY744419 vs. ExPASy Swiss-Prot
Match: CHIT_CARPA (Endochitinase OS=Carica papaya PE=1 SV=1)

HSP 1 Score: 103.219 bits (256), Expect = 2.310e-21
Identity = 49/107 (45.79%), Postives = 69/107 (64.49%), Query Frame = 2 
Query:  272 FLPNLAMKLLGGWTDAPDGEYAWGYCYITQVSPASSHYG-HPYWPCPLPGLYYGRGPIQLAWKCDYLVGGEALWVDLLNKPDLLATDPVLSSRSSI--WLLDDCPSP 583
            FL   + +  GGW  APDG YAWGYC++ + +P+S++    P +PC     YYGRGP+QL+W  +Y   GEAL V+LL  PDL+ATD VLS ++++  W+    P P
Sbjct:   60 FLGQTSHETTGGWPSAPDGPYAWGYCFLKERNPSSNYCAPSPRYPCAPGKSYYGRGPLQLSWNYNYGPCGEALRVNLLGNPDLVATDRVLSFKTALWFWMTPQAPKP 166
The following BLAST results are available for this feature:
BLAST of EY744419 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 45
Match NameE-valueIdentityDescription
CHI2_BRANA3.591e-2343.93Endochitinase CH25 OS=Brassica napus PE=2 SV=1[more]
CHI4_SOLTU3.596e-2346.23Endochitinase 4 (Fragment) OS=Solanum tuberosum GN... [more]
CHI1_TOBAC6.077e-2345.28Endochitinase A OS=Nicotiana tabacum GN=CHN48 PE=1... [more]
CHI2_TOBAC6.080e-2345.28Endochitinase B OS=Nicotiana tabacum GN=CHN50 PE=1... [more]
CHI3_TOBAC2.947e-2244.34Endochitinase 3 OS=Nicotiana tabacum GN=CHN14 PE=3... [more]
CHI9_ORYSJ4.989e-2241.51Chitinase 9 OS=Oryza sativa subsp. japonica GN=Cht... [more]
CHIT_SOLTU4.991e-2242.45Endochitinase OS=Solanum tuberosum PE=2 SV=1[more]
CHI1_HORVU1.862e-2144.8626 kDa endochitinase 1 OS=Hordeum vulgare PE=2 SV=... [more]
CHI11_ORYSJ1.872e-2140.00Chitinase 11 OS=Oryza sativa subsp. japonica GN=Ch... [more]
CHIT_CARPA2.310e-2145.79Endochitinase OS=Carica papaya PE=1 SV=1[more]

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Properties
Property NameValue
Genbank descriptionCS00-C5-003-008-D08-CT.F Sweet orange flower, greenhouse plant Citrus sinensis cDNA, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>EY744419 ID=EY744419; Name=EY744419; organism=Citrus sinensis; type=EST; length=962bp
CCACGCGTCCGGATAATTGATGTCGTACCCCTTGCTTTCAATGTGAGGCT
CATTGGGTCCTTACTAATTCTCTCTCTAGATTTATCTTTTGTACTAAGAG
GTTCAGCACAGAATTGAGGAAGCGGCGTTGTGTGCGGGGAACGTGATACT
GGCCACAGGACGGATGGCGGTGAACTGGGCAAGATCGTCTCACGGGAAAT
GTTTGAACACTTGCTTCCGGATACAAATGATGCCCGATGCGCTGCCACAG
GCTTCTACACTTATGATGCTTTTTTTGCCCAATCTGGCCATGAAACTACT
TGGGGGATGGACCGATGCACCTGATGGAGAATATGCTTGGGGATATTGCT
ATATTACGCAAGTGAGCCCTGCGTCGTCCCACTACGGTCATCCTTACTGG
CCATGTCCCTTGCCAGGGCTATACTATGGCCGAGGTCCCATTCAACTCGC
TTGGAAGTGCGATTACTTAGTGGGCGGAGAAGCCTTGTGGGTGGACTTGT
TGAACAAACCAGACCTACTTGCCACTGATCCTGTACTATCATCTCGGTCA
TCCATCTGGTTACTGGATGACTGCCCATCCCCACAAGCCATCCTGCCACC
AAGTCTCTATTGACTCATGGAAAACCCTTCTCCCAACTGAACCAACCTGC
CGAGCCGGCTTCCTGAATTACAGTCTTAACCAGGAATTTGATCAAGGTGG
GGGATTCCTCTGGGTCGAACTCTGGAGAATGCACCCCACCTCCCACAATG
GGTGGGGCAACTTTCCCCTGTTGGGGCGCCGAGGCCTCGGAACTAGACCC
CGGTGGAATAGCTCCTAGACACTTGGCGGAGACCATAAGATGAGCGCCCG
CTGCACCAAGCCCCTACCAATTCCTCCAGGACAAGACCGCTCCCCCGCGC
CTGCCACTCCCCTTACCCCACCGTCAACAGAACACCCTCCTGCTTCCTCT
ACCACGCACATG
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