EY663270 | Citrus Genome Database

Overview


Name	EY663270
Unique Name	EY663270
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	904

Libraries


Library Name	Type
Sweet orange leaf, infected with Xylella fastidiosa (stage 1 of 2)	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Homology

[Close]

BLAST of EY663270 vs. ExPASy Swiss-Prot
Match: GLNA_FUSSH (Glutamine synthetase OS=Fusarium solani subsp. phaseoli GN=GLN1 PE=2 SV=1)

HSP 1 Score: 113.235 bits (282), Expect = 1.240e-31
Identity = 53/118 (44.92%), Postives = 77/118 (65.25%), Query Frame = 1

Query:  298 KIIAEYIWIGGTGIDMRSKSKTISKPVEHPSELPKWNYDGSSTGQAPGEDSEVILYPQAIFKDPFRGGNNILVICDTYTPAGEPIPTNKRHRAAEIFSNSKVSAEVPWFGIEQEYTLL 651
            +I+AEY+W+   G + RSKS+T+ +      +LP WN+DGSST QAPG++S+V L P A++ DPFRG  NI+V+ + +   G P   N RH   ++      + + PWFG+EQEYTLL
Sbjct:   25 QIMAEYVWVDAAG-ETRSKSRTLPEKDYKAEDLPVWNFDGSSTNQAPGDNSDVYLRPCAVYPDPFRGSPNIIVLAECWNAGGTPNKFNFRHDCVKVMDT--YAEDEPWFGLEQEYTLL 139

HSP 2 Score: 45.0542 bits (105), Expect = 1.240e-31
Identity = 21/54 (38.89%), Postives = 24/54 (44.44%), Query Frame = 2

Query:  665 NGPLSWPVGAYPGPQGPYYWGXXXXXXXXXXXXXXLYKACLDGCINIRGTTGKL 826
            N P  WP G +P PQG YY G               YKACL   I I GT  ++
Sbjct:  143 NRPYGWPTGGFPAPQGEYYCGVGTGKVVQRDIVEAHYKACLYAGIQISGTNAEV 196

[Close]

BLAST of EY663270 vs. ExPASy Swiss-Prot
Match: GLNA_CHICK (Glutamine synthetase OS=Gallus gallus GN=GLUL PE=2 SV=1)

HSP 1 Score: 112.849 bits (281), Expect = 1.486e-31
Identity = 56/122 (45.90%), Postives = 77/122 (63.11%), Query Frame = 1

Query:  286 PYTDKIIAEYIWIGGTGIDMRSKSKTISKPVEHPSELPKWNYDGSSTGQAPGEDSEVILYPQAIFKDPFRGGNNILVICDTYTPAGEPIPTNKRHRAAEIFSNSKVSAEVPWFGIEQEYTLL 651
            P  +K+ A YIWI GTG  +R K++T+    +   +LP+WN+DGSST QA G +S++ L P A+F+DPFR   N LV+C+ +    +   TN RH    I     VS + PWFG+EQEYTLL
Sbjct:   21 PQGEKVQAMYIWIDGTGEHLRCKTRTLDHEPKSLEDLPEWNFDGSSTFQAEGSNSDMYLRPAAMFRDPFRKDPNKLVLCEVFKYNRQSADTNLRHTCRRIM--DMVSNQHPWFGMEQEYTLL 140

HSP 2 Score: 44.2838 bits (103), Expect = 1.486e-31
Identity = 19/52 (36.54%), Postives = 24/52 (46.15%), Query Frame = 2

Query:  671 PLSWPVGAYPGPQGPYYWGXXXXXXXXXXXXXXLYKACLDGCINIRGTTGKL 826
            P  WP   +PGPQGPYY G               Y+ACL   + I GT  ++
Sbjct:  146 PFGWPSNCFPGPQGPYYCGVGADKAYGRDIVEAHYRACLYAGVKIGGTNAEV 197

HSP 3 Score: 21.1718 bits (43), Expect = 1.486e-31
Identity = 6/10 (60.00%), Postives = 8/10 (80.00%), Query Frame = 3

Query:  828 PGQWEFKLAP 857
            P QWEF++ P
Sbjct:  199 PAQWEFQVGP 208

[Close]

BLAST of EY663270 vs. ExPASy Swiss-Prot
Match: GLNA_CRYNE (Glutamine synthetase OS=Cryptococcus neoformans GN=GLN1 PE=2 SV=2)

HSP 1 Score: 117.087 bits (292), Expect = 1.488e-31
Identity = 58/117 (49.57%), Postives = 78/117 (66.67%), Query Frame = 1

Query:  298 KIIAEYIWIGGTGIDMRSKSKTISKPVEHPSELPKWNYDGSSTGQAPGEDSEVILYPQAIFKDPFRGGNNILVICDTYTPAGEPIPTNKRHRAAEIFSNSKVSAEVPWFGIEQEYTL 648
            ++ AEYIWI   G  +RSK+ T+ K     ++L +WN+DGSST QAP ++S+V L P AIFKDPFRGG NILV+C+ Y   G P  +N R    ++   +K +   PWFG+EQEYTL
Sbjct:   24 RVQAEYIWIDAEG-GIRSKTMTLDKAPSSVADLKEWNFDGSSTNQAPADNSDVFLRPVAIFKDPFRGGANILVLCECYDNDGTPNKSNYRAHCKKVMDAAKDTE--PWFGLEQEYTL 137

HSP 2 Score: 40.0466 bits (92), Expect = 1.488e-31
Identity = 18/49 (36.73%), Postives = 22/49 (44.90%), Query Frame = 2

Query:  680 WPVGAYPGPQGPYYWGXXXXXXXXXXXXXXLYKACLDGCINIRGTTGKL 826
            WP   +PGPQGPYY G               Y+ACL   I I G   ++
Sbjct:  147 WPKNGFPGPQGPYYCGVGAGKVFARDFIEAHYRACLYAGIKISGINAEV 195

HSP 3 Score: 21.1718 bits (43), Expect = 1.488e-31
Identity = 6/10 (60.00%), Postives = 8/10 (80.00%), Query Frame = 3

Query:  828 PGQWEFKLAP 857
            P QWEF++ P
Sbjct:  197 PSQWEFQVGP 206

[Close]

BLAST of EY663270 vs. ExPASy Swiss-Prot
Match: GLNA_MACFA (Glutamine synthetase OS=Macaca fascicularis GN=GLUL PE=2 SV=3)

HSP 1 Score: 112.464 bits (280), Expect = 2.101e-31
Identity = 57/123 (46.34%), Postives = 79/123 (64.23%), Query Frame = 1

Query:  286 PYTDKIIAEYIWIGGTGIDMRSKSKTISKPVEHPSELPKWNYDGSSTGQAPGEDSEVILYPQAIFKDPFRGGNNILVICDTYTPAGE-PIPTNKRHRAAEIFSNSKVSAEVPWFGIEQEYTLL 651
            P  +K+ A YIWI GTG  +R K++T+    +   ELP+WN+DGSST Q+ G +S++ L P A+F+DPFR   N LV+C+++    E P  TN RH    I     VS + PWFG+EQEYTL+
Sbjct:   21 PQGEKVQAMYIWIDGTGEGLRCKTRTLDSEPKCVEELPEWNFDGSSTLQSEGSNSDMYLVPAAMFRDPFRKDPNKLVLCESFQVQFEGPAETNLRHTCKRIM--DMVSNQHPWFGMEQEYTLM 141

HSP 2 Score: 45.0542 bits (105), Expect = 2.101e-31
Identity = 24/69 (34.78%), Postives = 30/69 (43.48%), Query Frame = 2

Query:  671 PLSWPVGAYPGPQGPYYWGXXXXXXXXXXXXXXLYKACLDGCINIRGTTGKL---SWAVGI*AGPREAL 868
            P  WP   +PGPQGPYY G               Y+ACL   + I GT  ++    W   I  GP E +
Sbjct:  147 PFGWPSNGFPGPQGPYYCGVGADRAYGRDIVEAHYRACLYAGVKIAGTNAEVMPAQWEFQI--GPCEGI 213

[Close]

BLAST of EY663270 vs. ExPASy Swiss-Prot
Match: GLNA1_DROME (Glutamine synthetase 1, mitochondrial OS=Drosophila melanogaster GN=Gs1 PE=2 SV=3)

HSP 1 Score: 119.013 bits (297), Expect = 2.501e-31
Identity = 63/133 (47.37%), Postives = 90/133 (67.67%), Query Frame = 1

Query:  256 LEDLLNLDITPYTDKIIAEYIWIGGTGIDMRSKSKTISKPVEHPSELPKWNYDGSSTGQAPGEDSEVILYPQAIFKDPFR-GGNNILVICDTYTPAGEPIPTNKRHRAAEIFSNSKVSAEVPWFGIEQEYTLL 651
            L+   NL+ TP  +++ A Y+WI GTG ++R K + + K      +LP W YDGSST QA GE+S+  L P+AI++DPF+ G N+++V+CDTY+  G+P  +NK  RAA   +   +S + PWFGIEQEYTLL
Sbjct:   49 LQRYRNLE-TP-ANRVQATYLWIDGTGENIRLKDRVLDKVPSSVEDLPDWQYDGSSTYQAHGENSDTTLKPRAIYRDPFKPGKNDVIVLCDTYSADGKPTASNK--RAAFQAAIDLISDQEPWFGIEQEYTLL 177

HSP 2 Score: 36.965 bits (84), Expect = 2.501e-31
Identity = 17/52 (32.69%), Postives = 21/52 (40.38%), Query Frame = 2

Query:  671 PLSWPVGAYPGPQGPYYWGXXXXXXXXXXXXXXLYKACLDGCINIRGTTGKL 826
            P  WP   +P PQGPYY G                 ACL   I+  GT  ++
Sbjct:  183 PFGWPENGFPAPQGPYYCGVGADRVYARDLVEAHVVACLYAGIDFAGTNAEV 234

HSP 3 Score: 21.557 bits (44), Expect = 2.501e-31
Identity = 6/10 (60.00%), Postives = 8/10 (80.00%), Query Frame = 3

Query:  828 PGQWEFKLAP 857
            P QWEF++ P
Sbjct:  236 PAQWEFQIGP 245

[Close]

BLAST of EY663270 vs. ExPASy Swiss-Prot
Match: GLNA_ACOCA (Glutamine synthetase OS=Acomys cahirinus GN=GLUL PE=2 SV=3)

HSP 1 Score: 111.694 bits (278), Expect = 3.563e-31
Identity = 55/122 (45.08%), Postives = 77/122 (63.11%), Query Frame = 1

Query:  286 PYTDKIIAEYIWIGGTGIDMRSKSKTISKPVEHPSELPKWNYDGSSTGQAPGEDSEVILYPQAIFKDPFRGGNNILVICDTYTPAGEPIPTNKRHRAAEIFSNSKVSAEVPWFGIEQEYTLL 651
            P  +K+ A YIW+ GTG  +R K++T+    +   ELP+WN+DGSST Q+ G +S++ L P A+F+DPFR   N LV C+ +    +P  TN RH    I     VS + PWFG+EQEYTL+
Sbjct:   21 PQGEKVQAMYIWVDGTGEGLRCKTRTLDCEPKCVEELPEWNFDGSSTFQSEGSNSDMYLSPVAMFRDPFRKEPNKLVFCEVFKYNRKPAETNLRHSCKRIM--DMVSNQHPWFGMEQEYTLM 140

HSP 2 Score: 45.0542 bits (105), Expect = 3.563e-31
Identity = 24/69 (34.78%), Postives = 30/69 (43.48%), Query Frame = 2

Query:  671 PLSWPVGAYPGPQGPYYWGXXXXXXXXXXXXXXLYKACLDGCINIRGTTGKL---SWAVGI*AGPREAL 868
            P  WP   +PGPQGPYY G               Y+ACL   + I GT  ++    W   I  GP E +
Sbjct:  146 PFGWPSNGFPGPQGPYYCGVGADKAYGRDIVEAHYRACLYAGVKITGTNAEVMPAQWEFQI--GPCEGI 212

[Close]

BLAST of EY663270 vs. ExPASy Swiss-Prot
Match: GLNA_GIBFU (Glutamine synthetase OS=Gibberella fujikuroi GN=GLN1 PE=3 SV=1)

HSP 1 Score: 109.383 bits (272), Expect = 1.738e-30
Identity = 58/137 (42.34%), Postives = 81/137 (59.12%), Query Frame = 1

Query:  241 STVNRLEDLLNLDITPYTDKIIAEYIWIGGTGIDMRSKSKTISKPVEHPSELPKWNYDGSSTGQAPGEDSEVILYPQAIFKDPFRGGNNILVICDTYTPAGEPIPTNKRHRAAEIFSNSKVSAEVPWFGIEQEYTLL 651
            S    L+  L LD       I+AEY+W+   G   RSKS+T+ +    P +LP WN+DGSST QAPG++S+V L P A++ DPFRG  NI+V+ + +   G P   N RH   ++      + + P FG+EQEYTLL
Sbjct:    7 SRTETLQKYLKLD---QKGMIMAEYVWVDADG-GTRSKSRTLPEKEYKPEDLPVWNFDGSSTNQAPGDNSDVYLRPCAVYPDPFRGSPNIIVLAECWNADGTPNKYNFRHDCVKVMDT--YADDEPLFGLEQEYTLL 137

HSP 2 Score: 45.0542 bits (105), Expect = 1.738e-30
Identity = 21/54 (38.89%), Postives = 24/54 (44.44%), Query Frame = 2

Query:  665 NGPLSWPVGAYPGPQGPYYWGXXXXXXXXXXXXXXLYKACLDGCINIRGTTGKL 826
            N P  WP G +P PQG YY G               YKACL   I I GT  ++
Sbjct:  141 NRPYGWPAGGFPAPQGEYYCGVGTGKVVQRDIVEAHYKACLYAGIQISGTNAEV 194

[Close]

BLAST of EY663270 vs. ExPASy Swiss-Prot
Match: GLNA_CRIGR (Glutamine synthetase OS=Cricetulus griseus GN=GLUL PE=2 SV=4)

HSP 1 Score: 110.153 bits (274), Expect = 2.433e-29
Identity = 55/122 (45.08%), Postives = 76/122 (62.30%), Query Frame = 1

Query:  286 PYTDKIIAEYIWIGGTGIDMRSKSKTISKPVEHPSELPKWNYDGSSTGQAPGEDSEVILYPQAIFKDPFRGGNNILVICDTYTPAGEPIPTNKRHRAAEIFSNSKVSAEVPWFGIEQEYTLL 651
            P  +K+ A YIW+ GTG  +R K++T+    +   ELP+WN+DGSST Q+   +S++ L P A+F+DPFR   N LV C+ +    +P  TN RH    I     VS + PWFG+EQEYTLL
Sbjct:   21 PQGEKVQAMYIWVDGTGEGLRCKTRTLDCEPKCVEELPEWNFDGSSTFQSESSNSDMYLSPVAMFRDPFRKEPNKLVFCEVFKYNQKPAETNLRHTCKRIM--DMVSNQHPWFGMEQEYTLL 140

HSP 2 Score: 40.4318 bits (93), Expect = 2.433e-29
Identity = 19/55 (34.55%), Postives = 24/55 (43.64%), Query Frame = 2

Query:  671 PLSWPVGAYPGPQGPYYWGXXXXXXXXXXXXXXLYKACLDGCINIRGTTGKLSWA 835
            P  WP   +PGPQG YY G               Y+ACL   + I GT  ++  A
Sbjct:  146 PFGWPSDGFPGPQGLYYCGVGADKAYRRDIMEAHYRACLYAGVKITGTYAEVKHA 200

[Close]

BLAST of EY663270 vs. ExPASy Swiss-Prot
Match: GLNA_XENLA (Glutamine synthetase OS=Xenopus laevis GN=glul PE=2 SV=1)

HSP 1 Score: 102.834 bits (255), Expect = 7.843e-29
Identity = 52/125 (41.60%), Postives = 75/125 (60.00%), Query Frame = 1

Query:  286 PYTDKIIAEYIWIGGTGIDMRSKSKTISKPVEHPSELPKWNYDGSSTGQAPGEDSEVILYPQAIFKDPFRGGNNILVICDTYTPAGEPIPTNKRHRAAEIFSNSKVSAEVPWFGIEQEYTLLQQN 660
            P  +K+   Y+WI GTG  +R K++T+ +  +   E+P+WN+DGSST QA G +S++ L P  +F+DPF    N LV+C+      +   TN RH   +I     V+   PWFG+EQEYTLL  N
Sbjct:   21 PQGEKVQVTYVWIDGTGEGVRCKTRTLDQEPKTIDEIPEWNFDGSSTHQAEGSNSDMYLIPVQMFRDPFCLDPNKLVMCEVLKYNRKSAETNLRHTCKKIM--EMVNDHRPWFGMEQEYTLLGIN 143

HSP 2 Score: 45.0542 bits (105), Expect = 7.843e-29
Identity = 21/52 (40.38%), Postives = 24/52 (46.15%), Query Frame = 2

Query:  671 PLSWPVGAYPGPQGPYYWGXXXXXXXXXXXXXXLYKACLDGCINIRGTTGKL 826
            P  WP   +PGPQGPYY G               YKACL   I I GT  ++
Sbjct:  146 PYGWPENGFPGPQGPYYCGVGADKVYGRDVVESHYKACLYAGIKICGTNAEV 197

HSP 3 Score: 21.1718 bits (43), Expect = 7.843e-29
Identity = 6/10 (60.00%), Postives = 8/10 (80.00%), Query Frame = 3

Query:  828 PGQWEFKLAP 857
            P QWEF++ P
Sbjct:  199 PSQWEFQVGP 208

[Close]

BLAST of EY663270 vs. ExPASy Swiss-Prot
Match: GLNA2_CHLRE (Glutamine synthetase, chloroplastic OS=Chlamydomonas reinhardtii GN=GLN2 PE=2 SV=1)

HSP 1 Score: 104.76 bits (260), Expect = 1.449e-26
Identity = 57/125 (45.60%), Postives = 77/125 (61.60%), Query Frame = 1

Query:  307 AEYIWIGGT------GI---DMRSKSKTISKPVE-HPSELPKWNYDGSSTGQAPGEDSEVILYPQAIFKDPFRGGNNILVICDTYTPAGEPIPTNKRHRAAEIFSNSKVSAEVPWFGIEQEYTLL 651
            AEYIW  G       G+   +MRSK+K    P+    SE P W++DGSSTGQA G +S+ IL P  +  DP RG  ++LV+C+ + P G+P  TN R +  EI  + KV+AE  W+G EQEYT+L
Sbjct:   37 AEYIWADGNEGKPEKGMIFNEMRSKTKCFEAPLGLDASEYPDWSFDGSSTGQAEGNNSDCILRPVRVVTDPIRGAPHVLVMCEVFAPDGKPHSTNTRAKLREII-DDKVTAEDCWYGFEQEYTML 160

HSP 2 Score: 33.8834 bits (76), Expect = 1.449e-26
Identity = 14/49 (28.57%), Postives = 21/49 (42.86%), Query Frame = 2

Query:  680 WPVGAYPGPQGPYYWGXXXXXXXXXXXXXXLYKACLDGCINIRGTTGKL 826
            WP G +P PQGP+Y G                +AC+   + I G   ++
Sbjct:  170 WPAGGFPAPQGPFYCGVGAESAFGRPLAEAHMEACMKAGLVISGINAEV 218

HSP 3 Score: 22.7126 bits (47), Expect = 1.449e-26
Identity = 6/10 (60.00%), Postives = 9/10 (90.00%), Query Frame = 3

Query:  828 PGQWEFKLAP 857
            PGQWE+++ P
Sbjct:  220 PGQWEYQIGP 229

The following BLAST results are available for this feature:

BLAST of EY663270 vs. ExPASy Swiss-Prot

BLAST of EY663270 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 86


Match Name	E-value	Identity	Description
GLNA_FUSSH	1.240e-31	44.92	Glutamine synthetase OS=Fusarium solani subsp. pha...	[more]
GLNA_CHICK	1.486e-31	45.90	Glutamine synthetase OS=Gallus gallus GN=GLUL PE=2...	[more]
GLNA_CRYNE	1.488e-31	49.57	Glutamine synthetase OS=Cryptococcus neoformans GN...	[more]
GLNA_MACFA	2.101e-31	46.34	Glutamine synthetase OS=Macaca fascicularis GN=GLU...	[more]
GLNA1_DROME	2.501e-31	47.37	Glutamine synthetase 1, mitochondrial OS=Drosophil...	[more]
GLNA_ACOCA	3.563e-31	45.08	Glutamine synthetase OS=Acomys cahirinus GN=GLUL P...	[more]
GLNA_GIBFU	1.738e-30	42.34	Glutamine synthetase OS=Gibberella fujikuroi GN=GL...	[more]
GLNA_CRIGR	2.433e-29	45.08	Glutamine synthetase OS=Cricetulus griseus GN=GLUL...	[more]
GLNA_XENLA	7.843e-29	41.60	Glutamine synthetase OS=Xenopus laevis GN=glul PE=...	[more]
GLNA2_CHLRE	1.449e-26	45.60	Glutamine synthetase, chloroplastic OS=Chlamydomon...	[more]

Pages

Properties


Property Name	Value
Genbank description	CS00-C1-101-053-D04-CT.F Sweet orange leaf, infected with Xylella fastidiosa (stage 1 of 2) Citrus sinensis cDNA, mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>EY663270 ID=EY663270; Name=EY663270; organism=Citrus sinensis; type=EST; length=904bp
GACCCTGCTTCGCATCAGCATTTTGGTTTTACTCTTCAACCAATTTTCAG
GGAGGAGTAGGTGAAAATGGCGCAGATTTTGGCACCTTCTATGCAATGGC
AGATGAGGATGCCTAAGTACTCAAACATTGCAAGTCCTATGACAACAAAG
ATGTGGAGTTCTCTGTTGATGAAGCAGAACAAGAAAGGAACAAATAGGAG
TTCTGCCAAGTTTAGAGTGCTTGCTTTGAAATCTGAAGACAGCACTGTCA
ATAGGCTAGAGGATCTACTCAATTTGGACATCACTCCATATACTGACAAA
ATCATTGCCGAATATATTTGGATTGGAGGTACTGGGATTGATATGCGTAG
CAAGTCAAAGACCATTTCAAAGCCTGTTGAGCATCCATCTGAGCTTCCCA
AGTGGAATTATGATGGATCAAGTACTGGACAGGCACCTGGTGAAGATAGT
GAAGTAATTTTATACCCTCAAGCAATTTTTAAGGACCCCTTCCGTGGAGG
AAACAACATCTTGGTAATTTGTGATACCTACACACCAGCAGGCGAGCCCA
TCCCTACAAACAAACGCCATAGGGCTGCTGAAATTTTCAGTAACTCGAAA
GTGTCTGCTGAAGTTCCATGGTTTGGGATAGAGCAAGAGTACACCCTACT
TCAACAAAATGTGGAATGGGCCTCTGAGTTGGCCTGTTGGAGCCTATCCC
GGTCCCCAGGGTCCTTATTACTGGGGAGCTGGGGCTGATAGGTCGTTTGC
GCGGGACTTTGCAGATGCTCTTTACAAGGCTTGCTTGGATGGCTGCATTA
ACATTAGGGGCACCACGGGGAAGTTATCCTGGGCAGTGGGAATTTAAGCT
GGCCCCAGGGAGGCATTATGCTGGAAAAAATAAGGGGGTCCAAAAACCTC
TTGA