EY663231 | Citrus Genome Database

Overview


Name	EY663231
Unique Name	EY663231
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	885

Libraries


Library Name	Type
Sweet orange leaf, infected with Xylella fastidiosa (stage 1 of 2)	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Homology

[Close]

BLAST of EY663231 vs. ExPASy Swiss-Prot
Match: CDKL2_MOUSE (Cyclin-dependent kinase-like 2 OS=Mus musculus GN=Cdkl2 PE=2 SV=1)

HSP 1 Score: 81.6481 bits (200), Expect = 1.394e-18
Identity = 37/73 (50.68%), Postives = 53/73 (72.60%), Query Frame = 3

Query:   12 CDF*RAKQLIA-GEANISYICSRFYRAPELIFGATEYTTSIDIWSAGCVLAELLLGQPLFPGENAVDQLVEII 227
            CDF  A+ L A GE    Y+ +R+YRAPEL+ G  +Y  ++DIW+ GC++ E+L+GQPLFPGE+ +DQL  I+
Sbjct:  143 CDFGFARTLAAPGEVYTDYVATRWYRAPELLVGDVKYGKAVDIWAIGCLVIEMLMGQPLFPGESDIDQLHHIM 215

HSP 2 Score: 32.7278 bits (73), Expect = 1.394e-18
Identity = 20/68 (29.41%), Postives = 29/68 (42.65%), Query Frame = 1

Query:  319 PTREEIRCMNPNYTDFRFPQIK---AHPWHKVFHKRMPPEAIDLASRLLQYSPSLRCTALEACAHPFF 513
            P  +E+   NP +   R P++K   A P    + K +P   I LA + L   P  R    +   H FF
Sbjct:  223 PRHQELFYKNPVFAGVRLPEVKDAEAEPLESRYPK-LPEAVISLAKKCLHIDPDKRPFCADLLRHDFF 289

[Close]

BLAST of EY663231 vs. ExPASy Swiss-Prot
Match: CDKL2_RAT (Cyclin-dependent kinase-like 2 OS=Rattus norvegicus GN=Cdkl2 PE=2 SV=1)

HSP 1 Score: 81.6481 bits (200), Expect = 1.398e-18
Identity = 37/73 (50.68%), Postives = 53/73 (72.60%), Query Frame = 3

Query:   12 CDF*RAKQLIA-GEANISYICSRFYRAPELIFGATEYTTSIDIWSAGCVLAELLLGQPLFPGENAVDQLVEII 227
            CDF  A+ L A GE    Y+ +R+YRAPEL+ G  +Y  ++DIW+ GC++ E+L+GQPLFPGE+ +DQL  I+
Sbjct:  143 CDFGFARTLAAPGEVYTDYVATRWYRAPELLVGDVKYGKAVDIWAIGCLVIEMLMGQPLFPGESDIDQLHHIM 215

HSP 2 Score: 32.7278 bits (73), Expect = 1.398e-18
Identity = 21/68 (30.88%), Postives = 29/68 (42.65%), Query Frame = 1

Query:  319 PTREEIRCMNPNYTDFRFPQIK---AHPWHKVFHKRMPPEAIDLASRLLQYSPSLRCTALEACAHPFF 513
            P  +E+   NP +   R P+IK   A P    + K +P   I LA + L   P  R    +   H FF
Sbjct:  223 PRHQELFYKNPVFAGVRLPEIKDIEAEPLESRYPK-LPEVVISLAKKCLHIDPDKRPLCADLLHHDFF 289

[Close]

BLAST of EY663231 vs. ExPASy Swiss-Prot
Match: MCK1_YEAST (Protein kinase MCK1 OS=Saccharomyces cerevisiae GN=MCK1 PE=1 SV=1)

HSP 1 Score: 93.5893 bits (231), Expect = 1.612e-18
Identity = 43/74 (58.11%), Postives = 55/74 (74.32%), Query Frame = 3

Query:   12 CDF*RAKQLIAGEANISYICSRFYRAPELIFGATEYTTSIDIWSAGCVLAELLLGQPLFPGENAVDQLVEIIKV 233
            CDF  AK+L   + +ISYICSRFYRAPELI G T+YTT IDIW  GCV+ E+L+G+ +F G+  + QL EI K+
Sbjct:  182 CDFGSAKKLEHNQPSISYICSRFYRAPELIIGCTQYTTQIDIWGLGCVMGEMLIGKAIFQGQEPLLQLREIAKL 255

[Close]

BLAST of EY663231 vs. ExPASy Swiss-Prot
Match: CDKL2_RABIT (Cyclin-dependent kinase-like 2 OS=Oryctolagus cuniculus GN=CDKL2 PE=2 SV=1)

HSP 1 Score: 79.7221 bits (195), Expect = 3.065e-18
Identity = 34/74 (45.95%), Postives = 54/74 (72.97%), Query Frame = 3

Query:   12 CDF*RAKQLIA-GEANISYICSRFYRAPELIFGATEYTTSIDIWSAGCVLAELLLGQPLFPGENAVDQLVEIIK 230
            CDF  A+ L A GE    Y+ +R+YRAPEL+ G  +Y  ++D+W+ GC++ E+L+G+PLFPG++ +DQL  I++
Sbjct:  143 CDFGFARTLAAPGEVYTDYVATRWYRAPELLVGDVKYGKAVDVWAIGCLVTEMLMGEPLFPGDSDIDQLYLIMR 216

HSP 2 Score: 33.4982 bits (75), Expect = 3.065e-18
Identity = 19/65 (29.23%), Postives = 28/65 (43.08%), Query Frame = 1

Query:  319 PTREEIRCMNPNYTDFRFPQIKAHPWHKVFHKRMPPEAIDLASRLLQYSPSLRCTALEACAHPFF 513
            P  +E+   NP +   R P+IK     +  + ++    IDLA + L   P  R    E   H FF
Sbjct:  223 PRHQELFYKNPVFAGVRLPEIKESEPLERRYPKLSEVVIDLAKKCLHVDPDKRPFCAELLHHDFF 287

[Close]

BLAST of EY663231 vs. ExPASy Swiss-Prot
Match: MK01_HUMAN (Mitogen-activated protein kinase 1 OS=Homo sapiens GN=MAPK1 PE=1 SV=3)

HSP 1 Score: 71.633 bits (174), Expect = 3.097e-18
Identity = 29/57 (50.88%), Postives = 44/57 (77.19%), Query Frame = 3

Query:   63 YICSRFYRAPELIFGATEYTTSIDIWSAGCVLAELLLGQPLFPGENAVDQLVEIIKV 233
            Y+ +R+YRAPE++  +  YT SIDIWS GC+LAE+L  +P+FPG++ +DQL  I+ +
Sbjct:  187 YVATRWYRAPEIMLNSKGYTKSIDIWSVGCILAEMLSNRPIFPGKHYLDQLNHILGI 243

HSP 2 Score: 41.5874 bits (96), Expect = 3.097e-18
Identity = 21/78 (26.92%), Postives = 42/78 (53.85%), Query Frame = 1

Query:  313 GTPTREEIRCM----NPNYTDFRFPQIKAHPWHKVFHKRMPPEAIDLASRLLQYSPSLRCTALEACAHPFFDELREPN 534
            G+P++E++ C+      NY     P     PW+++F      +A+DL  ++L ++P  R    +A AHP+ ++  +P+
Sbjct:  245 GSPSQEDLNCIINLKARNYL-LSLPHKNKVPWNRLF-PNADSKALDLLDKMLTFNPHKRIEVEQALAHPYLEQYYDPS 320

[Close]

BLAST of EY663231 vs. ExPASy Swiss-Prot
Match: MK01_BOVIN (Mitogen-activated protein kinase 1 OS=Bos taurus GN=MAPK1 PE=2 SV=3)

HSP 1 Score: 71.633 bits (174), Expect = 3.097e-18
Identity = 29/57 (50.88%), Postives = 44/57 (77.19%), Query Frame = 3

Query:   63 YICSRFYRAPELIFGATEYTTSIDIWSAGCVLAELLLGQPLFPGENAVDQLVEIIKV 233
            Y+ +R+YRAPE++  +  YT SIDIWS GC+LAE+L  +P+FPG++ +DQL  I+ +
Sbjct:  187 YVATRWYRAPEIMLNSKGYTKSIDIWSVGCILAEMLSNRPIFPGKHYLDQLNHILGI 243

HSP 2 Score: 41.5874 bits (96), Expect = 3.097e-18
Identity = 21/78 (26.92%), Postives = 42/78 (53.85%), Query Frame = 1

Query:  313 GTPTREEIRCM----NPNYTDFRFPQIKAHPWHKVFHKRMPPEAIDLASRLLQYSPSLRCTALEACAHPFFDELREPN 534
            G+P++E++ C+      NY     P     PW+++F      +A+DL  ++L ++P  R    +A AHP+ ++  +P+
Sbjct:  245 GSPSQEDLNCIINLKARNYL-LSLPHKNKVPWNRLF-PNADSKALDLLDKMLTFNPHKRIEVEQALAHPYLEQYYDPS 320

[Close]

BLAST of EY663231 vs. ExPASy Swiss-Prot
Match: MK01_RAT (Mitogen-activated protein kinase 1 OS=Rattus norvegicus GN=Mapk1 PE=1 SV=3)

HSP 1 Score: 71.633 bits (174), Expect = 3.097e-18
Identity = 29/57 (50.88%), Postives = 44/57 (77.19%), Query Frame = 3

Query:   63 YICSRFYRAPELIFGATEYTTSIDIWSAGCVLAELLLGQPLFPGENAVDQLVEIIKV 233
            Y+ +R+YRAPE++  +  YT SIDIWS GC+LAE+L  +P+FPG++ +DQL  I+ +
Sbjct:  185 YVATRWYRAPEIMLNSKGYTKSIDIWSVGCILAEMLSNRPIFPGKHYLDQLNHILGI 241

HSP 2 Score: 41.5874 bits (96), Expect = 3.097e-18
Identity = 21/78 (26.92%), Postives = 42/78 (53.85%), Query Frame = 1

Query:  313 GTPTREEIRCM----NPNYTDFRFPQIKAHPWHKVFHKRMPPEAIDLASRLLQYSPSLRCTALEACAHPFFDELREPN 534
            G+P++E++ C+      NY     P     PW+++F      +A+DL  ++L ++P  R    +A AHP+ ++  +P+
Sbjct:  243 GSPSQEDLNCIINLKARNYL-LSLPHKNKVPWNRLF-PNADSKALDLLDKMLTFNPHKRIEVEQALAHPYLEQYYDPS 318

[Close]

BLAST of EY663231 vs. ExPASy Swiss-Prot
Match: MK01_MOUSE (Mitogen-activated protein kinase 1 OS=Mus musculus GN=Mapk1 PE=1 SV=3)

HSP 1 Score: 71.633 bits (174), Expect = 3.097e-18
Identity = 29/57 (50.88%), Postives = 44/57 (77.19%), Query Frame = 3

Query:   63 YICSRFYRAPELIFGATEYTTSIDIWSAGCVLAELLLGQPLFPGENAVDQLVEIIKV 233
            Y+ +R+YRAPE++  +  YT SIDIWS GC+LAE+L  +P+FPG++ +DQL  I+ +
Sbjct:  185 YVATRWYRAPEIMLNSKGYTKSIDIWSVGCILAEMLSNRPIFPGKHYLDQLNHILGI 241

HSP 2 Score: 41.5874 bits (96), Expect = 3.097e-18
Identity = 21/78 (26.92%), Postives = 42/78 (53.85%), Query Frame = 1

Query:  313 GTPTREEIRCM----NPNYTDFRFPQIKAHPWHKVFHKRMPPEAIDLASRLLQYSPSLRCTALEACAHPFFDELREPN 534
            G+P++E++ C+      NY     P     PW+++F      +A+DL  ++L ++P  R    +A AHP+ ++  +P+
Sbjct:  243 GSPSQEDLNCIINLKARNYL-LSLPHKNKVPWNRLF-PNADSKALDLLDKMLTFNPHKRIEVEQALAHPYLEQYYDPS 318

[Close]

BLAST of EY663231 vs. ExPASy Swiss-Prot
Match: MAPK_FUSSO (Mitogen-activated protein kinase OS=Fusarium solani subsp. pisi GN=MAPK PE=2 SV=1)

HSP 1 Score: 74.7146 bits (182), Expect = 3.098e-18
Identity = 36/78 (46.15%), Postives = 51/78 (65.38%), Query Frame = 3

Query:   12 CDF*RAKQLIAGEAN----ISYICSRFYRAPELIFGATEYTTSIDIWSAGCVLAELLLGQPLFPGENAVDQLVEIIKV 233
            CDF  A+   + E N      Y+ +R+YRAPE++    EYT +ID+WS GC+LAE+L G+PLFPG++   QL  I+ V
Sbjct:  164 CDFGLARSAASQEDNSGFMTEYVATRWYRAPEIMLTFKEYTKAIDVWSVGCILAEMLSGKPLFPGKDYHHQLTLILDV 241

HSP 2 Score: 38.5058 bits (88), Expect = 3.098e-18
Identity = 28/90 (31.11%), Postives = 40/90 (44.44%), Query Frame = 1

Query:  313 GTPTREE---IRCMNPNYTDFRFPQIKAHPWHKVFHKRMPPEAIDLASRLLQYSPSLRCTALEACAHPFFDELREPNARLPNGRPLPPLF 573
            GTPT E+   I+           P  K  P+  +F K     A+DL  +LL ++P  R T  EA  HP+ +   +P    P   P+P  F
Sbjct:  243 GTPTMEDYYGIKSRRAREYIRSLPFKKKVPFRTLFPKTSDL-ALDLLEKLLAFNPVKRITVEEALKHPYLEPYHDPEDE-PTAPPIPEEF 330

[Close]

BLAST of EY663231 vs. ExPASy Swiss-Prot
Match: MK15_RAT (Mitogen-activated protein kinase 15 OS=Rattus norvegicus GN=Mapk15 PE=1 SV=2)

HSP 1 Score: 72.0182 bits (175), Expect = 3.988e-18
Identity = 34/79 (43.04%), Postives = 49/79 (62.03%), Query Frame = 3

Query:   12 CDF*RAKQLI------AGEANISYICSRFYRAPELIFGATEYTTSIDIWSAGCVLAELLLGQPLFPGENAVDQLVEIIK 230
            CDF  A+ L        G+A   Y+ +R+YRAPE++  +  YT  +D+WS GC+L E+L GQPLFPG +   QL  I++
Sbjct:  155 CDFGLARSLSDFPEGPGGQALTEYVATRWYRAPEVLLSSRWYTPGVDMWSLGCILGEMLRGQPLFPGTSTFHQLELILE 233

HSP 2 Score: 40.817 bits (94), Expect = 3.988e-18
Identity = 22/81 (27.16%), Postives = 38/81 (46.91%), Query Frame = 1

Query:  319 PTREEIRCMNPNYTDFRFPQIKAHPWHKV---FHKRMPPEAIDLASRLLQYSPSLRCTALEACAHPFFDELREPNARLPNG 552
            P+ EE++ +  +Y+      + + P   +        PPEA+DL  RLL ++P  R +A +A  HP+      P+     G
Sbjct:  238 PSMEELQGLGSDYSALILQNLGSRPRQTLDALLPPDTPPEALDLLKRLLAFAPDKRLSAEQALQHPYVQRFHCPDREWTRG 318

The following BLAST results are available for this feature:

BLAST of EY663231 vs. ExPASy Swiss-Prot

BLAST of EY663231 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 272


Match Name	E-value	Identity	Description
CDKL2_MOUSE	1.394e-18	50.68	Cyclin-dependent kinase-like 2 OS=Mus musculus GN=...	[more]
CDKL2_RAT	1.398e-18	50.68	Cyclin-dependent kinase-like 2 OS=Rattus norvegicu...	[more]
MCK1_YEAST	1.612e-18	58.11	Protein kinase MCK1 OS=Saccharomyces cerevisiae GN...	[more]
CDKL2_RABIT	3.065e-18	45.95	Cyclin-dependent kinase-like 2 OS=Oryctolagus cuni...	[more]
MK01_HUMAN	3.097e-18	50.88	Mitogen-activated protein kinase 1 OS=Homo sapiens...	[more]
MK01_BOVIN	3.097e-18	50.88	Mitogen-activated protein kinase 1 OS=Bos taurus G...	[more]
MK01_RAT	3.097e-18	50.88	Mitogen-activated protein kinase 1 OS=Rattus norve...	[more]
MK01_MOUSE	3.097e-18	50.88	Mitogen-activated protein kinase 1 OS=Mus musculus...	[more]
MAPK_FUSSO	3.098e-18	46.15	Mitogen-activated protein kinase OS=Fusarium solan...	[more]
MK15_RAT	3.988e-18	43.04	Mitogen-activated protein kinase 15 OS=Rattus norv...	[more]

Pages

Properties


Property Name	Value
Genbank description	CS00-C1-101-052-H11-CT.F Sweet orange leaf, infected with Xylella fastidiosa (stage 1 of 2) Citrus sinensis cDNA, mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>EY663231 ID=EY663231; Name=EY663231; organism=Citrus sinensis; type=EST; length=885bp
CCACGCGTCCGTGTGATTTTTGACGTGCAAAACAGCTGATTGCTGGAGAA
GCAAACATATCATACATATGTTCACGTTTCTATCGGGCTCCAGAACTTAT
ATTTGGAGCTACAGAATATACAACCTCAATTGATATATGGTCAGCTGGTT
GTGTGCTTGCCGAGCTTCTTTTGGGCCAGCCATTATTTCCTGGAGAAAAT
GCAGTGGACCAGCTTGTAGAGATTATCAAGGTACAGTTTGCCCTCAAATT
TTTGAAATGATGCAATTTTCATGATCACTCGTCACATGCTGCTTTTGCTT
CTGTAGGTTCTCGGTACACCCACTCGTGAAGAAATTCGCTGTATGAATCC
AAATTATACCGATTTTAGGTTTCCACAGATTAAAGCACATCCTTGGCACA
AGGTTTTCCACAAACGGATGCCTCCTGAGGCTATTGATCTTGCTTCACGC
CTGTTGCAGTATTCACCAAGTCTCCGATGTACAGCGCTAGAAGCATGTGC
ACATCCTTTCTTTGATGAACTGCGTGAGCCAAATGCTCGCCTACCAAATG
GCCGCCCACTGCCACCTCTCTTCAACTATAAACAGGAATTATCGGGGGCT
TCACCATAGCTTGTTAATAAGTTGATACCCAGACCATGTGAAACGGCAAT
TAGGTCTGAATTTCTTGCATCCAGCCGGGACATACGTCTAGACAAGTAAT
ATAGAAGTGAAATCTACTCGGTGATGTGTAACACAAACGGCATTATATCC
TTGTATCGAACTGCCATGCATTGAGGCCTTATCACCATCCAGTGTTGCTC
GATCAGCAAGCCTACTTTCTCAGGCAGCTATGCTGTTCTTGGGGCCCTCT
TTTTCTGCTGTTCATCCTCCGGTGTCTCCTCCCAC