EY662311 | Citrus Genome Database

Overview


Name	EY662311
Unique Name	EY662311
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	901

Libraries


Library Name	Type
Sweet orange leaf, infected with Xylella fastidiosa (stage 1 of 2)	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Alignments

Feature Name	Type	Location	Analysis
EY662311_ssr92	microsatellite	EY662311_ssr92:92..115.	BLAST: Citrus ESTs to Prunus persica proteins V1
EY662311_ssr112	microsatellite	EY662311_ssr112:112..123.	BLAST: Citrus ESTs to Prunus persica proteins V1
Csv1_Contig9730	contig	Csv1_Contig9730:1..890.	BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1

Homology

[Close]

BLAST of EY662311 vs. ExPASy Swiss-Prot
Match: AQP1_BOVIN (Aquaporin-1 OS=Bos taurus GN=AQP1 PE=1 SV=3)

HSP 1 Score: 113.235 bits (282), Expect = 1.413e-26
Identity = 71/180 (39.44%), Postives = 104/180 (57.78%), Query Frame = 3

Query:  216 AELKLWSFYRALIAEFVATLLFLYVSVATVIGHK---KQSDACGGV-GLLGIAWAFGGMIFVLVYCTAGISGGHINPAVTFGLFLARKVSLIRAVAYMVAQCLGAICGVGLVKAFMKH-EYNSLGGGANTVASGYNKGSALGAEIIGTFVLVYTVFSATDPKRSARDSPVPLLAPLPLGF 740
            +E K   F+RA++AEF+A +LF+++S+ + +G     K +   G V   + ++ AFG  I  L      ISG H+NPAVT GL L+ ++S++RA+ Y++AQC+GAI    ++         NSL  G N +A G N G  LG EIIGT  LV  V + TD +R  RD  +    PL +GF
Sbjct:    3 SEFKKKLFWRAVVAEFLAMILFIFISIGSALGFHYPIKSNQTTGAVQDNVKVSLAFGLSIATLAQSVGHISGAHLNPAVTLGLLLSCQISVLRAIMYIIAQCVGAIVATAILSGITSSLPDNSL--GLNALAPGVNSGQGLGIEIIGTLQLVLCVLATTDRRR--RD--LGGSGPLAIGF 176

HSP 2 Score: 28.1054 bits (61), Expect = 1.413e-26
Identity = 14/25 (56.00%), Postives = 17/25 (68.00%), Query Frame = 1

Query:  751 GSLGPIPLPGTGINPAWSFGAAVIS 825
            G L  I   G GINPA SFG++VI+
Sbjct:  181 GHLLAIDYTGCGINPARSFGSSVIT 205

[Close]

BLAST of EY662311 vs. ExPASy Swiss-Prot
Match: AQP1_HUMAN (Aquaporin-1 OS=Homo sapiens GN=AQP1 PE=1 SV=3)

HSP 1 Score: 112.079 bits (279), Expect = 1.413e-26
Identity = 66/170 (38.82%), Postives = 96/170 (56.47%), Query Frame = 3

Query:  216 AELKLWSFYRALIAEFVATLLFLYVSVATVIGHKKQ--SDACGGVGLLGIAWAFGGMIFVLVYCTAGISGGHINPAVTFGLFLARKVSLIRAVAYMVAQCLGAICGVGLVKAFMKH-EYNSLGGGANTVASGYNKGSALGAEIIGTFVLVYTVFSATDPKRSARDSPVPL 716
            +E K   F+RA++AEF+AT LF+++S+ + +G K    ++       + ++ AFG  I  L      ISG H+NPAVT GL L+ ++S+ RA+ Y++AQC+GAI    ++         NSL  G N +A G N G  LG EIIGT  LV  V + TD +R       PL
Sbjct:    3 SEFKKKLFWRAVVAEFLATTLFVFISIGSALGFKYPVGNNQTAVQDNVKVSLAFGLSIATLAQSVGHISGAHLNPAVTLGLLLSCQISIFRALMYIIAQCVGAIVATAILSGITSSLTGNSL--GRNDLADGVNSGQGLGIEIIGTLQLVLCVLATTDRRRRDLGGSAPL 170

HSP 2 Score: 29.261 bits (64), Expect = 1.413e-26
Identity = 15/25 (60.00%), Postives = 17/25 (68.00%), Query Frame = 1

Query:  751 GSLGPIPLPGTGINPAWSFGAAVIS 825
            G L  I   G GINPA SFG+AVI+
Sbjct:  179 GHLLAIDYTGCGINPARSFGSAVIT 203

[Close]

BLAST of EY662311 vs. ExPASy Swiss-Prot
Match: AQP1_SHEEP (Aquaporin-1 OS=Ovis aries GN=AQP1 PE=2 SV=3)

HSP 1 Score: 112.849 bits (281), Expect = 1.839e-26
Identity = 71/180 (39.44%), Postives = 104/180 (57.78%), Query Frame = 3

Query:  216 AELKLWSFYRALIAEFVATLLFLYVSVATVIGHK---KQSDACGGV-GLLGIAWAFGGMIFVLVYCTAGISGGHINPAVTFGLFLARKVSLIRAVAYMVAQCLGAICGVGLVKAFMKH-EYNSLGGGANTVASGYNKGSALGAEIIGTFVLVYTVFSATDPKRSARDSPVPLLAPLPLGF 740
            +E K   F+RA++AEF+A +LF+++S+ + +G     K +   G V   + ++ AFG  I  L      ISG H+NPAVT GL L+ ++S++RA+ Y++AQC+GAI    ++         NSL  G N +A G N G  LG EIIGT  LV  V + TD +R  RD  +    PL +GF
Sbjct:    3 SEFKKKLFWRAVVAEFLAMILFIFISIGSALGFHYPIKSNQTTGAVQDNVKVSLAFGLSIATLAQSVGHISGAHLNPAVTLGLLLSCQISILRAIMYIIAQCVGAIVATVILSGITSSLPDNSL--GLNALAPGVNSGQGLGIEIIGTLQLVLCVLATTD-RRRRRD--LGDSGPLAIGF 177

HSP 2 Score: 28.1054 bits (61), Expect = 1.839e-26
Identity = 14/25 (56.00%), Postives = 17/25 (68.00%), Query Frame = 1

Query:  751 GSLGPIPLPGTGINPAWSFGAAVIS 825
            G L  I   G GINPA SFG++VI+
Sbjct:  182 GHLLAIDYTGCGINPARSFGSSVIT 206

[Close]

BLAST of EY662311 vs. ExPASy Swiss-Prot
Match: AQP1_CANFA (Aquaporin-1 OS=Canis familiaris GN=AQP1 PE=2 SV=3)

HSP 1 Score: 111.694 bits (278), Expect = 4.056e-26
Identity = 66/172 (38.37%), Postives = 96/172 (55.81%), Query Frame = 3

Query:  216 AELKLWSFYRALIAEFVATLLFLYVSVATVIGH----KKQSDACGGVGLLGIAWAFGGMIFVLVYCTAGISGGHINPAVTFGLFLARKVSLIRAVAYMVAQCLGAICGVGLVKAFMKH-EYNSLGGGANTVASGYNKGSALGAEIIGTFVLVYTVFSATDPKRSARDSPVPL 716
            +E K   F+RA++AEF+A +LF+++S+ + +G     +    A      + ++ AFG  I  L      ISG H+NPAVT GL L+ ++S++RAV Y++AQC+GAI    ++         NSL  G N +A G N G  LG EIIGT  LV  V + TD +R       PL
Sbjct:    3 SEFKKKLFWRAVVAEFLAMILFVFISIGSALGFNYPVRNNQTAGAAQDNVKVSLAFGLSIATLAQSVGHISGAHLNPAVTLGLLLSCQISILRAVMYIIAQCVGAIVATAILSGITSSLPDNSL--GRNELAPGVNSGQGLGIEIIGTLQLVLCVLATTDRRRRDLGGSGPL 172

HSP 2 Score: 28.1054 bits (61), Expect = 4.056e-26
Identity = 14/25 (56.00%), Postives = 17/25 (68.00%), Query Frame = 1

Query:  751 GSLGPIPLPGTGINPAWSFGAAVIS 825
            G L  I   G GINPA SFG++VI+
Sbjct:  181 GHLLAIDYTGCGINPARSFGSSVIT 205

[Close]

BLAST of EY662311 vs. ExPASy Swiss-Prot
Match: AQP1_PIG (Aquaporin-1 OS=Sus scrofa GN=AQP1 PE=2 SV=3)

HSP 1 Score: 110.538 bits (275), Expect = 4.056e-26
Identity = 70/180 (38.89%), Postives = 105/180 (58.33%), Query Frame = 3

Query:  216 AELKLWSFYRALIAEFVATLLFLYVSVATVIGHK---KQSDACGGV-GLLGIAWAFGGMIFVLVYCTAGISGGHINPAVTFGLFLARKVSLIRAVAYMVAQCLGAICGVGLVKAFMKH-EYNSLGGGANTVASGYNKGSALGAEIIGTFVLVYTVFSATDPKRSARDSPVPLLAPLPLGF 740
            +E K   F+RA++AEF+A  LF+++S+ + +G +   + +   G     + ++ AFG  I  L      ISG H+NPAVT GL L+ ++S++RAV Y++AQC+GAI    ++         NSL  G N++A G + G  LG EIIGT  LV  V + TD +R  RD  +   APL +GF
Sbjct:    3 SEFKKKIFWRAVVAEFLAMTLFIFISIGSALGFQYPVRNNQTSGAAQDNVKVSLAFGLSIATLAQSVGHISGAHLNPAVTLGLLLSCQISVLRAVMYIIAQCVGAIVATAILSGITSSLPGNSL--GLNSLAPGVDSGQGLGIEIIGTLQLVLCVLATTDRRR--RD--LGGSAPLAIGF 176

HSP 2 Score: 29.261 bits (64), Expect = 4.056e-26
Identity = 15/25 (60.00%), Postives = 17/25 (68.00%), Query Frame = 1

Query:  751 GSLGPIPLPGTGINPAWSFGAAVIS 825
            G L  I   G GINPA SFG+AVI+
Sbjct:  181 GHLLAIDYTGCGINPARSFGSAVIT 205

[Close]

BLAST of EY662311 vs. ExPASy Swiss-Prot
Match: AQP1_PONAB (Aquaporin-1 OS=Pongo abelii GN=AQP1 PE=2 SV=3)

HSP 1 Score: 109.768 bits (273), Expect = 6.872e-26
Identity = 65/170 (38.24%), Postives = 95/170 (55.88%), Query Frame = 3

Query:  216 AELKLWSFYRALIAEFVATLLFLYVSVATVIGHKKQ--SDACGGVGLLGIAWAFGGMIFVLVYCTAGISGGHINPAVTFGLFLARKVSLIRAVAYMVAQCLGAICGVGLVKAFMKH-EYNSLGGGANTVASGYNKGSALGAEIIGTFVLVYTVFSATDPKRSARDSPVPL 716
            +E K   F+RA++AEF+A  LF+++S+ + +G K    ++       + ++ AFG  I  L      ISG H+NPAVT GL L+ ++S+ RA+ Y++AQC+GAI    ++         NSL  G N +A G N G  LG EIIGT  LV  V + TD +R       PL
Sbjct:    3 SEFKKKLFWRAVVAEFLAMTLFVFISIGSALGFKYPVGNNQTAVQDNVKVSLAFGLSIATLAQSVGHISGAHLNPAVTLGLLLSCQISIFRALMYIIAQCVGAIVATAILSGITSSLPGNSL--GRNDLADGVNSGQGLGIEIIGTLQLVLCVLATTDRRRRDLGGSAPL 170

HSP 2 Score: 29.261 bits (64), Expect = 6.872e-26
Identity = 15/25 (60.00%), Postives = 17/25 (68.00%), Query Frame = 1

Query:  751 GSLGPIPLPGTGINPAWSFGAAVIS 825
            G L  I   G GINPA SFG+AVI+
Sbjct:  179 GHLLAIDYTGCGINPARSFGSAVIT 203

[Close]

BLAST of EY662311 vs. ExPASy Swiss-Prot
Match: AQP1_MOUSE (Aquaporin-1 OS=Mus musculus GN=Aqp1 PE=1 SV=3)

HSP 1 Score: 109.768 bits (273), Expect = 1.164e-25
Identity = 67/173 (38.73%), Postives = 97/173 (56.07%), Query Frame = 3

Query:  216 AELKLWSFYRALIAEFVATLLFLYVSVATVIG-----HKKQSDACGGVGLLGIAWAFGGMIFVLVYCTAGISGGHINPAVTFGLFLARKVSLIRAVAYMVAQCLGAICGVGLVKAFMKHEY-NSLGGGANTVASGYNKGSALGAEIIGTFVLVYTVFSATDPKRSARDSPVPL 716
            +E+K   F+RA++AEF+A  LF+++S+ + +G      + Q+     V    ++ AFG  I  L      ISG H+NPAVT GL L+ ++S++RAV Y++AQC+GAI    ++         NSL  G N +A G N G  LG EIIGT  LV  V + TD +R       PL
Sbjct:    3 SEIKKKLFWRAVVAEFLAMTLFVFISIGSALGFNYPLERNQTLVQDNVK---VSLAFGLSIATLAQSVGHISGAHLNPAVTLGLLLSCQISILRAVMYIIAQCVGAIVATAILSGITSSLVDNSL--GRNDLAHGVNSGQGLGIEIIGTLQLVLCVLATTDRRRRDLGGSAPL 170

HSP 2 Score: 28.4906 bits (62), Expect = 1.164e-25
Identity = 14/25 (56.00%), Postives = 17/25 (68.00%), Query Frame = 1

Query:  751 GSLGPIPLPGTGINPAWSFGAAVIS 825
            G L  I   G GINPA SFG+AV++
Sbjct:  179 GHLLAIDYTGCGINPARSFGSAVLT 203

[Close]

BLAST of EY662311 vs. ExPASy Swiss-Prot
Match: AQP1_RAT (Aquaporin-1 OS=Rattus norvegicus GN=Aqp1 PE=2 SV=4)

HSP 1 Score: 108.997 bits (271), Expect = 1.972e-25
Identity = 67/173 (38.73%), Postives = 97/173 (56.07%), Query Frame = 3

Query:  216 AELKLWSFYRALIAEFVATLLFLYVSVATVIG-----HKKQSDACGGVGLLGIAWAFGGMIFVLVYCTAGISGGHINPAVTFGLFLARKVSLIRAVAYMVAQCLGAICGVGLVKAFMKHEY-NSLGGGANTVASGYNKGSALGAEIIGTFVLVYTVFSATDPKRSARDSPVPL 716
            +E+K   F+RA++AEF+A  LF+++S+ + +G      + Q+     V    ++ AFG  I  L      ISG H+NPAVT GL L+ ++S++RAV Y++AQC+GAI    ++         NSL  G N +A G N G  LG EIIGT  LV  V + TD +R       PL
Sbjct:    3 SEIKKKLFWRAVVAEFLAMTLFVFISIGSALGFNYPLERNQTLVQDNVK---VSLAFGLSIATLAQSVGHISGAHLNPAVTLGLLLSCQISILRAVMYIIAQCVGAIVASAILSGITSSLLENSL--GRNDLARGVNSGQGLGIEIIGTLQLVLCVLATTDRRRRDLGGSAPL 170

HSP 2 Score: 28.4906 bits (62), Expect = 1.972e-25
Identity = 14/25 (56.00%), Postives = 17/25 (68.00%), Query Frame = 1

Query:  751 GSLGPIPLPGTGINPAWSFGAAVIS 825
            G L  I   G GINPA SFG+AV++
Sbjct:  179 GHLLAIDYTGCGINPARSFGSAVLT 203

[Close]

BLAST of EY662311 vs. ExPASy Swiss-Prot
Match: AQPA_RANES (Aquaporin FA-CHIP OS=Rana esculenta GN=AQPA PE=2 SV=1)

HSP 1 Score: 109.383 bits (272), Expect = 3.340e-25
Identity = 63/174 (36.21%), Postives = 100/174 (57.47%), Query Frame = 3

Query:  216 AELKLWSFYRALIAEFVATLLFLYVSVATVIG-----HKKQSDACG-GVGLLGIAWAFGGMIFVLVYCTAGISGGHINPAVTFGLFLARKVSLIRAVAYMVAQCLGAICGVGLVKAFMKH-EYNSLGGGANTVASGYNKGSALGAEIIGTFVLVYTVFSATDPKRSARDSPVPL 716
            +E K  +F+RA+IAEF+A +LF+++S+   +G      +K +   G    ++ ++ AFG  I  +      +SG H+NPAVT G  L+ ++S+++AV Y++AQCLGA+    ++       E NSL  G N ++ G + G  LG EI+ TF LV  V + TD +R      VPL
Sbjct:    3 SEFKKKAFWRAVIAEFLAMILFVFISIGAALGFNFPIEEKANQTVGRSQDIVKVSLAFGISIATMAQSVGHVSGAHLNPAVTLGCLLSCQISILKAVMYIIAQCLGAVVATAILSGITSGLENNSL--GLNGLSPGVSAGQGLGVEILVTFQLVLCVVAVTDRRRHDVSGSVPL 174

HSP 2 Score: 27.335 bits (59), Expect = 3.340e-25
Identity = 13/25 (52.00%), Postives = 17/25 (68.00%), Query Frame = 1

Query:  751 GSLGPIPLPGTGINPAWSFGAAVIS 825
            G L  I   G G+NPA SFG+AV++
Sbjct:  183 GHLIAIDYTGCGMNPARSFGSAVLT 207

[Close]

BLAST of EY662311 vs. ExPASy Swiss-Prot
Match: AQP4_NOTAL (Aquaporin-4 OS=Notomys alexis GN=AQP4 PE=2 SV=1)

HSP 1 Score: 106.301 bits (264), Expect = 1.330e-23
Identity = 62/154 (40.26%), Postives = 93/154 (60.39%), Query Frame = 3

Query:  234 SFYRALIAEFVATLLFLYVSVATVIGHKKQSDACGGVGLLGIAWAFGGMIFVLVYCTAGISGGHINPAVTFGLFLARKVSLIRAVAYMVAQCLGAICGVGLVKAFMKHEYNSLGG-GANTVASGYNKGSALGAEIIGTFVLVYTVFSATDPKRS 692
            +F++A+ AEF+A L+F+ +SV + I      +    V ++ I+  FG  I  +V C   ISGGHINPAVT  +   RK+S+ ++V Y+ AQCLGAI G G++  ++    N +GG G  TV      G  L  E+I TF LV+T+F++ D KR+
Sbjct:   36 AFWKAVTAEFLAMLIFVLLSVGSTINWGGSENPLP-VDMVLISLCFGLSIATMVQCFGHISGGHINPAVTVAMVCTRKISIAKSVFYITAQCLGAIIGAGIL--YLVTPPNVVGGLGVTTVHGNLTAGHGLLVELIITFQLVFTIFASCDSKRT 186

HSP 2 Score: 25.0238 bits (53), Expect = 1.330e-23
Identity = 13/24 (54.17%), Postives = 14/24 (58.33%), Query Frame = 1

Query:  751 GSLGPIPLPGTGINPAWSFGAAVI 822
            G L  I   G  +NPA SFG AVI
Sbjct:  203 GHLFAINYTGASMNPARSFGPAVI 226

The following BLAST results are available for this feature:

BLAST of EY662311 vs. ExPASy Swiss-Prot

BLAST of EY662311 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 160


Match Name	E-value	Identity	Description
AQP1_BOVIN	1.413e-26	39.44	Aquaporin-1 OS=Bos taurus GN=AQP1 PE=1 SV=3	[more]
AQP1_HUMAN	1.413e-26	38.82	Aquaporin-1 OS=Homo sapiens GN=AQP1 PE=1 SV=3	[more]
AQP1_SHEEP	1.839e-26	39.44	Aquaporin-1 OS=Ovis aries GN=AQP1 PE=2 SV=3	[more]
AQP1_CANFA	4.056e-26	38.37	Aquaporin-1 OS=Canis familiaris GN=AQP1 PE=2 SV=3	[more]
AQP1_PIG	4.056e-26	38.89	Aquaporin-1 OS=Sus scrofa GN=AQP1 PE=2 SV=3	[more]
AQP1_PONAB	6.872e-26	38.24	Aquaporin-1 OS=Pongo abelii GN=AQP1 PE=2 SV=3	[more]
AQP1_MOUSE	1.164e-25	38.73	Aquaporin-1 OS=Mus musculus GN=Aqp1 PE=1 SV=3	[more]
AQP1_RAT	1.972e-25	38.73	Aquaporin-1 OS=Rattus norvegicus GN=Aqp1 PE=2 SV=4	[more]
AQPA_RANES	3.340e-25	36.21	Aquaporin FA-CHIP OS=Rana esculenta GN=AQPA PE=2 S...	[more]
AQP4_NOTAL	1.330e-23	40.26	Aquaporin-4 OS=Notomys alexis GN=AQP4 PE=2 SV=1	[more]

Pages

Properties


Property Name	Value
Genbank description	CS00-C1-101-042-G06-CT.F Sweet orange leaf, infected with Xylella fastidiosa (stage 1 of 2) Citrus sinensis cDNA, mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>EY662311 ID=EY662311; Name=EY662311; organism=Citrus sinensis; type=EST; length=901bp
TTTTCGAACAGGGGGCGTCCGAGCTTTTCTCACACCAGAGAGGGTGTGTG
CTTTTCTTTGATTGTTCCAAAGAGAAATCAAAATTTTGTGCAGAAGAAGA
AGAAGAAGAAGAAGAAAGAAAGATGTCAAAGGAAGTGAATGAAGAAGGAC
AAACTCACCGCCATCACCATGGCAAGGACTACGTGGACCCACCACCAGCA
CCACTCATTGACATGGCTGAGCTCAAGCTCTGGTCCTTTTACAGAGCTCT
CATAGCCGAGTTCGTGGCCACTCTCCTCTTCCTCTACGTCAGTGTTGCCA
CTGTCATTGGCCACAAGAAACAAAGTGATGCCTGTGGTGGTGTTGGCCTT
TTGGGTATTGCATGGGCCTTTGGTGGCATGATCTTCGTCCTTGTTTACTG
CACCGCTGGCATTTCTGGTGGTCACATTAACCCTGCTGTGACGTTTGGGC
TGTTCTTGGCAAGGAAGGTATCATTGATCAGGGCTGTGGCTTACATGGTG
GCTCAGTGCTTGGGTGCCATTTGCGGTGTTGGTTTGGTCAAGGCCTTCAT
GAAGCATGAGTACAACTCACTTGGTGGTGGTGCTAACACTGTGGCTTCTG
GCTACAACAAAGGATCAGCCTTGGGTGCTGAGATTATTGGTACCTTCGTG
CTTGTTTACACAGTCTTCTCGGCCACAGACCCCAAGAGAAGCGCTCGTGA
CTCTCCCGTGCCCCTTTTGGCTCCACTTCCATTGGGATTTGTGTGGTCAT
GGGTCACTTGGCCCCATCCCCCTCCCTGGAACGGGCATCAACCCTGCCTG
GAGCTTTGGGGCTGCTGTTATCTCCACAATGACCAGGCCGGGGATGACCT
TGGATCTTCTGGGTGGTCCTTCCGTGGGACCTTGGCTGCCGCGGAATACA
A