EY744221 | Citrus Genome Database

Overview


Name	EY744221
Unique Name	EY744221
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	988

Libraries


Library Name	Type
Sweet orange flower, greenhouse plant	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Homology

BLAST of EY744221 vs. ExPASy Swiss-Prot
Match: NEDD8_CAEEL (NEDD8 OS=Caenorhabditis elegans GN=ned-8 PE=1 SV=1)

HSP 1 Score: 97.0561 bits (240), Expect = 1.721e-19
Identity = 46/76 (60.53%), Postives = 58/76 (76.32%), Query Frame = 3

Query:  333 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 560
            M I VKTLTGK I L++E +D ++ +K K+++KEGIPP QQRLIFAGKQ+ D +T ADY +   S LHLVL LRGG
Sbjct:    1 MLIKVKTLTGKEIELDIEPNDRVERIKEKVEEKEGIPPPQQRLIFAGKQMNDDKTAADYKVLGGSVLHLVLALRGG 76

HSP 2 Score: 94.7449 bits (234), Expect = 8.541e-19
Identity = 45/76 (59.21%), Postives = 57/76 (75.00%), Query Frame = 3

Query:  105 MQIFVKTLTGKTITLEVESSDTIDNVKAMIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 332
            M I VKTLTGK I L++E +D ++ +K  +++KEGIPP QQRLIFAGKQ+ D +T ADY +   S LHLVL LRGG
Sbjct:    1 MLIKVKTLTGKEIELDIEPNDRVERIKEKVEEKEGIPPPQQRLIFAGKQMNDDKTAADYKVLGGSVLHLVLALRGG 76

BLAST of EY744221 vs. ExPASy Swiss-Prot
Match: RUB2_ARATH (NEDD8-like protein RUB2 OS=Arabidopsis thaliana GN=RUB2 PE=2 SV=2)

HSP 1 Score: 96.6709 bits (239), Expect = 2.248e-19
Identity = 46/75 (61.33%), Postives = 59/75 (78.67%), Query Frame = 3

Query:  339 IFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGGM 563
            I VKTLTGK I +++E +DTID +K ++++KEGIPP QQRLI+AGKQL D +T  DY I+  S LHLVL LRGG+
Sbjct:    3 IKVKTLTGKEIEIDIEPTDTIDRIKERVEEKEGIPPVQQRLIYAGKQLADDKTAKDYAIEGGSVLHLVLALRGGL 77

HSP 2 Score: 95.5153 bits (236), Expect = 5.007e-19
Identity = 46/75 (61.33%), Postives = 58/75 (77.33%), Query Frame = 3

Query:  111 IFVKTLTGKTITLEVESSDTIDNVKAMIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGGM 335
            I VKTLTGK I +++E +DTID +K  +++KEGIPP QQRLI+AGKQL D +T  DY I+  S LHLVL LRGG+
Sbjct:    3 IKVKTLTGKEIEIDIEPTDTIDRIKERVEEKEGIPPVQQRLIYAGKQLADDKTAKDYAIEGGSVLHLVLALRGGL 77

BLAST of EY744221 vs. ExPASy Swiss-Prot
Match: NEDD8_RAT (NEDD8 OS=Rattus norvegicus GN=Nedd8 PE=1 SV=1)

HSP 1 Score: 93.2041 bits (230), Expect = 2.485e-18
Identity = 43/76 (56.58%), Postives = 58/76 (76.32%), Query Frame = 3

Query:  333 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 560
            M I VKTLTGK I +++E +D ++ +K ++++KEGIPP QQRLI++GKQ+ D +T ADY I   S LHLVL LRGG
Sbjct:    1 MLIKVKTLTGKEIEIDIEPTDKVERIKERVEEKEGIPPQQQRLIYSGKQMNDEKTAADYKILGGSVLHLVLALRGG 76

HSP 2 Score: 92.0485 bits (227), Expect = 5.536e-18
Identity = 43/76 (56.58%), Postives = 57/76 (75.00%), Query Frame = 3

Query:  105 MQIFVKTLTGKTITLEVESSDTIDNVKAMIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 332
            M I VKTLTGK I +++E +D ++ +K  +++KEGIPP QQRLI++GKQ+ D +T ADY I   S LHLVL LRGG
Sbjct:    1 MLIKVKTLTGKEIEIDIEPTDKVERIKERVEEKEGIPPQQQRLIYSGKQMNDEKTAADYKILGGSVLHLVLALRGG 76

BLAST of EY744221 vs. ExPASy Swiss-Prot
Match: NEDD8_RABIT (NEDD8 OS=Oryctolagus cuniculus GN=NEDD8 PE=3 SV=1)

HSP 1 Score: 93.2041 bits (230), Expect = 2.485e-18
Identity = 43/76 (56.58%), Postives = 58/76 (76.32%), Query Frame = 3

Query:  333 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 560
            M I VKTLTGK I +++E +D ++ +K ++++KEGIPP QQRLI++GKQ+ D +T ADY I   S LHLVL LRGG
Sbjct:    1 MLIKVKTLTGKEIEIDIEPTDKVERIKERVEEKEGIPPQQQRLIYSGKQMNDEKTAADYKILGGSVLHLVLALRGG 76

HSP 2 Score: 92.0485 bits (227), Expect = 5.536e-18
Identity = 43/76 (56.58%), Postives = 57/76 (75.00%), Query Frame = 3

Query:  105 MQIFVKTLTGKTITLEVESSDTIDNVKAMIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 332
            M I VKTLTGK I +++E +D ++ +K  +++KEGIPP QQRLI++GKQ+ D +T ADY I   S LHLVL LRGG
Sbjct:    1 MLIKVKTLTGKEIEIDIEPTDKVERIKERVEEKEGIPPQQQRLIYSGKQMNDEKTAADYKILGGSVLHLVLALRGG 76

BLAST of EY744221 vs. ExPASy Swiss-Prot
Match: NEDD8_MOUSE (NEDD8 OS=Mus musculus GN=Nedd8 PE=1 SV=2)

HSP 1 Score: 93.2041 bits (230), Expect = 2.485e-18
Identity = 43/76 (56.58%), Postives = 58/76 (76.32%), Query Frame = 3

Query:  333 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 560
            M I VKTLTGK I +++E +D ++ +K ++++KEGIPP QQRLI++GKQ+ D +T ADY I   S LHLVL LRGG
Sbjct:    1 MLIKVKTLTGKEIEIDIEPTDKVERIKERVEEKEGIPPQQQRLIYSGKQMNDEKTAADYKILGGSVLHLVLALRGG 76

HSP 2 Score: 92.0485 bits (227), Expect = 5.536e-18
Identity = 43/76 (56.58%), Postives = 57/76 (75.00%), Query Frame = 3

Query:  105 MQIFVKTLTGKTITLEVESSDTIDNVKAMIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 332
            M I VKTLTGK I +++E +D ++ +K  +++KEGIPP QQRLI++GKQ+ D +T ADY I   S LHLVL LRGG
Sbjct:    1 MLIKVKTLTGKEIEIDIEPTDKVERIKERVEEKEGIPPQQQRLIYSGKQMNDEKTAADYKILGGSVLHLVLALRGG 76

BLAST of EY744221 vs. ExPASy Swiss-Prot
Match: NEDD8_HUMAN (NEDD8 OS=Homo sapiens GN=NEDD8 PE=1 SV=1)

HSP 1 Score: 93.2041 bits (230), Expect = 2.485e-18
Identity = 43/76 (56.58%), Postives = 58/76 (76.32%), Query Frame = 3

Query:  333 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 560
            M I VKTLTGK I +++E +D ++ +K ++++KEGIPP QQRLI++GKQ+ D +T ADY I   S LHLVL LRGG
Sbjct:    1 MLIKVKTLTGKEIEIDIEPTDKVERIKERVEEKEGIPPQQQRLIYSGKQMNDEKTAADYKILGGSVLHLVLALRGG 76

HSP 2 Score: 92.0485 bits (227), Expect = 5.536e-18
Identity = 43/76 (56.58%), Postives = 57/76 (75.00%), Query Frame = 3

Query:  105 MQIFVKTLTGKTITLEVESSDTIDNVKAMIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 332
            M I VKTLTGK I +++E +D ++ +K  +++KEGIPP QQRLI++GKQ+ D +T ADY I   S LHLVL LRGG
Sbjct:    1 MLIKVKTLTGKEIEIDIEPTDKVERIKERVEEKEGIPPQQQRLIYSGKQMNDEKTAADYKILGGSVLHLVLALRGG 76

BLAST of EY744221 vs. ExPASy Swiss-Prot
Match: NEDD8_BOVIN (NEDD8 OS=Bos taurus GN=NEDD8 PE=3 SV=1)

HSP 1 Score: 93.2041 bits (230), Expect = 2.485e-18
Identity = 43/76 (56.58%), Postives = 58/76 (76.32%), Query Frame = 3

Query:  333 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 560
            M I VKTLTGK I +++E +D ++ +K ++++KEGIPP QQRLI++GKQ+ D +T ADY I   S LHLVL LRGG
Sbjct:    1 MLIKVKTLTGKEIEIDIEPTDKVERIKERVEEKEGIPPQQQRLIYSGKQMNDEKTAADYKILGGSVLHLVLALRGG 76

HSP 2 Score: 92.0485 bits (227), Expect = 5.536e-18
Identity = 43/76 (56.58%), Postives = 57/76 (75.00%), Query Frame = 3

Query:  105 MQIFVKTLTGKTITLEVESSDTIDNVKAMIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 332
            M I VKTLTGK I +++E +D ++ +K  +++KEGIPP QQRLI++GKQ+ D +T ADY I   S LHLVL LRGG
Sbjct:    1 MLIKVKTLTGKEIEIDIEPTDKVERIKERVEEKEGIPPQQQRLIYSGKQMNDEKTAADYKILGGSVLHLVLALRGG 76

BLAST of EY744221 vs. ExPASy Swiss-Prot
Match: RUB3_ORYSJ (NEDD8-like protein RUB3 OS=Oryza sativa subsp. japonica GN=RUB3 PE=3 SV=1)

HSP 1 Score: 92.8189 bits (229), Expect = 3.246e-18
Identity = 41/76 (53.95%), Postives = 58/76 (76.32%), Query Frame = 3

Query:  333 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 560
            + + V+TLTGK I +++E +DT+D +K +++++EGIPP QQRLI+ GKQL D +T  DY I+  S LHLVL LRGG
Sbjct:    1 LNVKVRTLTGKEIDIDIEMTDTVDRIKERVEEREGIPPVQQRLIYGGKQLADDKTAHDYKIEAGSVLHLVLALRGG 76

HSP 2 Score: 91.6633 bits (226), Expect = 7.231e-18
Identity = 41/76 (53.95%), Postives = 57/76 (75.00%), Query Frame = 3

Query:  105 MQIFVKTLTGKTITLEVESSDTIDNVKAMIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 332
            + + V+TLTGK I +++E +DT+D +K  ++++EGIPP QQRLI+ GKQL D +T  DY I+  S LHLVL LRGG
Sbjct:    1 LNVKVRTLTGKEIDIDIEMTDTVDRIKERVEEREGIPPVQQRLIYGGKQLADDKTAHDYKIEAGSVLHLVLALRGG 76

BLAST of EY744221 vs. ExPASy Swiss-Prot
Match: RUB3_ARATH (NEDD8-like protein RUB3 OS=Arabidopsis thaliana GN=RUB3 PE=2 SV=1)

HSP 1 Score: 90.1225 bits (222), Expect = 2.104e-17
Identity = 42/76 (55.26%), Postives = 57/76 (75.00%), Query Frame = 3

Query:  333 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 560
            M+I VKTLT K I +E+E +DTI+ +K +I++KEGIPP  QR+++ GKQL D  T   YN+++ S LHLVL LRGG
Sbjct:    1 MEIKVKTLTEKQIDIEIELTDTIERIKERIEEKEGIPPVHQRIVYTGKQLADDLTAKHYNLERGSVLHLVLALRGG 76

HSP 2 Score: 88.9669 bits (219), Expect = 4.687e-17
Identity = 42/76 (55.26%), Postives = 56/76 (73.68%), Query Frame = 3

Query:  105 MQIFVKTLTGKTITLEVESSDTIDNVKAMIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 332
            M+I VKTLT K I +E+E +DTI+ +K  I++KEGIPP  QR+++ GKQL D  T   YN+++ S LHLVL LRGG
Sbjct:    1 MEIKVKTLTEKQIDIEIELTDTIERIKERIEEKEGIPPVHQRIVYTGKQLADDLTAKHYNLERGSVLHLVLALRGG 76

BLAST of EY744221 vs. ExPASy Swiss-Prot
Match: NEDD8_DICDI (NEDD8 OS=Dictyostelium discoideum GN=nedd8 PE=3 SV=2)

HSP 1 Score: 89.7373 bits (221), Expect = 2.748e-17
Identity = 41/77 (53.25%), Postives = 58/77 (75.32%), Query Frame = 3

Query:  333 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGGM 563
            M I VKTLTGK I ++++ +D I  +K ++++KEGIPP QQRLIF GKQ+ D +  ++Y+I+  S LHLVL LRGG+
Sbjct:    1 MLIKVKTLTGKEIEIDIDPTDKIQRIKERVEEKEGIPPSQQRLIFGGKQMGDDKPASEYSIEGGSVLHLVLALRGGL 77

HSP 2 Score: 88.5817 bits (218), Expect = 6.121e-17
Identity = 41/77 (53.25%), Postives = 57/77 (74.03%), Query Frame = 3

Query:  105 MQIFVKTLTGKTITLEVESSDTIDNVKAMIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGGM 335
            M I VKTLTGK I ++++ +D I  +K  +++KEGIPP QQRLIF GKQ+ D +  ++Y+I+  S LHLVL LRGG+
Sbjct:    1 MLIKVKTLTGKEIEIDIDPTDKIQRIKERVEEKEGIPPSQQRLIFGGKQMGDDKPASEYSIEGGSVLHLVLALRGGL 77

The following BLAST results are available for this feature:

BLAST of EY744221 vs. ExPASy Swiss-Prot

BLAST of EY744221 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 99


Match Name	E-value	Identity	Description
NEDD8_CAEEL	1.721e-19	60.53	NEDD8 OS=Caenorhabditis elegans GN=ned-8 PE=1 SV=1	[more]
RUB2_ARATH	2.248e-19	61.33	NEDD8-like protein RUB2 OS=Arabidopsis thaliana GN...	[more]
NEDD8_RAT	2.485e-18	56.58	NEDD8 OS=Rattus norvegicus GN=Nedd8 PE=1 SV=1	[more]
NEDD8_RABIT	2.485e-18	56.58	NEDD8 OS=Oryctolagus cuniculus GN=NEDD8 PE=3 SV=1	[more]
NEDD8_MOUSE	2.485e-18	56.58	NEDD8 OS=Mus musculus GN=Nedd8 PE=1 SV=2	[more]
NEDD8_HUMAN	2.485e-18	56.58	NEDD8 OS=Homo sapiens GN=NEDD8 PE=1 SV=1	[more]
NEDD8_BOVIN	2.485e-18	56.58	NEDD8 OS=Bos taurus GN=NEDD8 PE=3 SV=1	[more]
RUB3_ORYSJ	3.246e-18	53.95	NEDD8-like protein RUB3 OS=Oryza sativa subsp. jap...	[more]
RUB3_ARATH	2.104e-17	55.26	NEDD8-like protein RUB3 OS=Arabidopsis thaliana GN...	[more]
NEDD8_DICDI	2.748e-17	53.25	NEDD8 OS=Dictyostelium discoideum GN=nedd8 PE=3 SV...	[more]

Pages

Properties


Property Name	Value
Genbank description	CS00-C5-003-001-C03-CT.F Sweet orange flower, greenhouse plant Citrus sinensis cDNA, mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>EY744221 ID=EY744221; Name=EY744221; organism=Citrus sinensis; type=EST; length=988bp
CGGTCGGGTCCGGGTCCCGGAATTCCGGGTCGACCCCGCGTCCGCATCTT
GATCGCAACAATTATCAATTCTTCCTTCGTTCGCAAGAAAAAGCTCTGTT
CGATATGCAGATCTTCGTGAAAACCTTGACCGGAAAAACTATTACTCTCG
AAGTCGAGAGCTCGGACACCATCGACAACGTAAAGGCCATGATCCAAGAC
AAGGAGGGAATTCCGCCGGACCAGCAGAGGCTTATCTTTGCTGGAAAGCA
GCTCGAGGACGGCCGCACCTTGGCCGATTACAACATCCAGAAGGAGTCAA
CCCTTCATCTTGTCCTTCGTCTCCGCGGAGGCATGCAGATCTTCGTGAAA
ACCCTGACTGGCAAAACTATTACTCTCGAAGTCGAGAGCTCCGACACCAT
CGACAACGTCAAGGCGAAGATTCAAGACAAGGAGGGAATCCCCCCGGACC
AGCAGAGGCTTATCTTCGCTGGAAAGCAGCTCGAGGATGGCCGCACCTTG
GCCGATTACAACATACAGAAGGAGTCAACCCTCCATCTTGTCCTTCGTCT
CCGCGGAGGCATGCAGATCTTTGTCCAAACCCTTACCGGGAAGACGATAA
CCCTCGAGGTTGAGAGCTCAAACCCGATCGATAACGTTAAGGGCGAAGAT
TCAAGACAAGGGAGGAAATCCCCCCGGAACCACCAAAAGGCTGATTTTTT
GCTGGGCAAGCAACTGGGAAAAACGGCCGCACCTTGGGCTGGAATAACAA
TATCCCAAAAAGGGAGTCCAACCCTTTCTTCTCCCGCCCTCCGGGCTCCC
GGGGGGGGTAAGCAAAAATCTTCCCCAAAAACCCCCAAACGGGGGAAAAA
AAAATCCCCCCGGGGAGGGGGGAAAAATTTCGGGATACCAAATTTGAATA
ACCCCCCCGGGGCGCCAGAATTCTCCCGGAAAAAAAAAAAGGGGGGGGAT
AACCCCCCCGCGGACCACCTCCAAAAAAAAATTTTTTA