EY660988

Overview
NameEY660988
Unique NameEY660988
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length916
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of EY660988 vs. ExPASy Swiss-Prot
Match: ISPF_HAEIG (2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase OS=Haemophilus influenzae (strain PittGG) GN=ispF PE=3 SV=1)

HSP 1 Score: 132.88 bits (333), Expect = 2.532e-30
Identity = 63/127 (49.61%), Postives = 86/127 (67.72%), Query Frame = 3 
Query:  315 RVGHGFDLHRLEPGYPLIIGGINVPHERGCEAHSDGDVLLHCVVDAILGALGLPDIGQIFPDSDPKWKGAPSSVFIKEAVRLMDEAGYEIGNLDATLILQRPKLSPYKETIRTNLSELLGADPTVVN 695
            R+GHGFD+H      PLIIGG+ VP+  G  AHSDGDV LH + DAILGA  L DIG++FPD+D ++K A S   ++EA R + E GY+IGN+D T+I Q PK+ P+ + +R  ++E +  D   VN
Sbjct:    3 RIGHGFDVHAFGEDRPLIIGGVEVPYHTGFIAHSDGDVALHALTDAILGAAALGDIGKLFPDTDMQYKNADSRGLLREAFRQVQEKGYKIGNVDITIIAQAPKMRPHIDAMRAKIAEDIQCDIEQVN 129
[Close]
BLAST of EY660988 vs. ExPASy Swiss-Prot
Match: ISPF_EDWI9 (2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase OS=Edwardsiella ictaluri (strain 93-146) GN=ispF PE=3 SV=1)

HSP 1 Score: 132.88 bits (333), Expect = 2.532e-30
Identity = 62/117 (52.99%), Postives = 81/117 (69.23%), Query Frame = 3 
Query:  315 RVGHGFDLHRLEPGYPLIIGGINVPHERGCEAHSDGDVLLHCVVDAILGALGLPDIGQIFPDSDPKWKGAPSSVFIKEAVRLMDEAGYEIGNLDATLILQRPKLSPYKETIRTNLSE 665
            R+GHGFD+HR     PLIIGG+ + H +G  AHSDGDV LH   DA+LGA  L DIG++FPD+DP +KGA S   ++EA R +   GY +GNLD TLI Q PK++P+   +R  L+E
Sbjct:    2 RIGHGFDVHRFGGEGPLIIGGVRIAHPQGLLAHSDGDVALHAATDALLGAAALGDIGKLFPDTDPAYKGADSRALLREAYRSIRARGYRLGNLDITLIAQAPKMAPHIPQMRVFLAE 118
[Close]
BLAST of EY660988 vs. ExPASy Swiss-Prot
Match: ISPF_CHRVO (2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase OS=Chromobacterium violaceum GN=ispF PE=3 SV=1)

HSP 1 Score: 132.88 bits (333), Expect = 2.532e-30
Identity = 61/128 (47.66%), Postives = 89/128 (69.53%), Query Frame = 3 
Query:  312 FRVGHGFDLHRLEPGYPLIIGGINVPHERGCEAHSDGDVLLHCVVDAILGALGLPDIGQIFPDSDPKWKGAPSSVFIKEAVRLMDEAGYEIGNLDATLILQRPKLSPYKETIRTNLSELLGADPTVVN 695
            FR+G G+D+H+L  G PLI+GG+++PH++G   HSD D LLH + DA+LGA  L DIG+ FPD+  ++KGA S   ++EA   +  AG+   N+D+TLI QRPKL+P+ + +R N++  LG D   VN
Sbjct:    2 FRIGQGYDVHQLVEGRPLILGGVDIPHDKGLLGHSDADALLHAITDALLGAAALGDIGRHFPDTAAEFKGADSRALLREAAARVRAAGWRPVNVDSTLIAQRPKLAPHIDAMRANIAADLGLDVGAVN 129
[Close]
BLAST of EY660988 vs. ExPASy Swiss-Prot
Match: ISPF_ACTSZ (2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase OS=Actinobacillus succinogenes (strain ATCC 55618 / 130Z) GN=ispF PE=3 SV=1)

HSP 1 Score: 132.88 bits (333), Expect = 2.532e-30
Identity = 62/127 (48.82%), Postives = 87/127 (68.50%), Query Frame = 3 
Query:  315 RVGHGFDLHRLEPGYPLIIGGINVPHERGCEAHSDGDVLLHCVVDAILGALGLPDIGQIFPDSDPKWKGAPSSVFIKEAVRLMDEAGYEIGNLDATLILQRPKLSPYKETIRTNLSELLGADPTVVN 695
            R+GHGFD+H L    PLIIGG+ VP+  G  AHSDGDV+LH + DA+LGA+ L DIG++FPD+D ++K   S + ++EA R + E GY +GN+D T+I Q PK+ PY + +R  ++E L      VN
Sbjct:    3 RIGHGFDVHALGEARPLIIGGVKVPYHTGFIAHSDGDVVLHALTDALLGAVALGDIGKLFPDTDMQFKDIDSRILLREAFRRVKEKGYAVGNVDVTIIAQAPKMRPYIDAMRAVIAEDLQCGIDQVN 129
[Close]
BLAST of EY660988 vs. ExPASy Swiss-Prot
Match: ISPF_SYNP6 (2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase OS=Synechococcus sp. (strain ATCC 27144 / PCC 6301 / SAUG 1402/1) GN=ispF PE=3 SV=1)

HSP 1 Score: 132.494 bits (332), Expect = 3.307e-30
Identity = 58/124 (46.77%), Postives = 87/124 (70.16%), Query Frame = 3 
Query:  312 FRVGHGFDLHRLEPGYPLIIGGINVPHERGCEAHSDGDVLLHCVVDAILGALGLPDIGQIFPDSDPKWKGAPSSVFIKEAVRLMDEAGYEIGNLDATLILQRPKLSPYKETIRTNLSELLGADP 683
            FR+G+G+D+HRL  G PLI+GGI + H  G + HSD DVL H ++DA+LGAL L DIG  FP +DP+WKGA S   + +  +L+ + G+ IGNLD+ ++ ++PKL P+   +R  L+++L  +P
Sbjct:    4 FRIGNGYDIHRLVEGRPLILGGIQLEHSLGLDGHSDADVLTHAIMDALLGALSLGDIGHYFPPTDPQWKGADSLKLLAQVNQLIRDRGWTIGNLDSVVVAEQPKLKPHIAAMRDRLAKVLELEP 127
[Close]
BLAST of EY660988 vs. ExPASy Swiss-Prot
Match: ISPF_SYNE7 (2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase OS=Synechococcus elongatus (strain PCC 7942) GN=ispF PE=3 SV=1)

HSP 1 Score: 132.494 bits (332), Expect = 3.307e-30
Identity = 58/124 (46.77%), Postives = 87/124 (70.16%), Query Frame = 3 
Query:  312 FRVGHGFDLHRLEPGYPLIIGGINVPHERGCEAHSDGDVLLHCVVDAILGALGLPDIGQIFPDSDPKWKGAPSSVFIKEAVRLMDEAGYEIGNLDATLILQRPKLSPYKETIRTNLSELLGADP 683
            FR+G+G+D+HRL  G PLI+GGI + H  G + HSD DVL H ++DA+LGAL L DIG  FP +DP+WKGA S   + +  +L+ + G+ IGNLD+ ++ ++PKL P+   +R  L+++L  +P
Sbjct:    4 FRIGNGYDIHRLVEGRPLILGGIQLEHSLGLDGHSDADVLTHAIMDALLGALSLGDIGHYFPPTDPQWKGADSLKLLAQVNQLIRDRGWTIGNLDSVVVAEQPKLKPHIAAMRDRLAKVLELEP 127
[Close]
BLAST of EY660988 vs. ExPASy Swiss-Prot
Match: ISPF_SHESR (2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase OS=Shewanella sp. (strain MR-7) GN=ispF PE=3 SV=1)

HSP 1 Score: 132.494 bits (332), Expect = 3.307e-30
Identity = 63/130 (48.46%), Postives = 87/130 (66.92%), Query Frame = 3 
Query:  306 LPFRVGHGFDLHRLEPGYPLIIGGINVPHERGCEAHSDGDVLLHCVVDAILGALGLPDIGQIFPDSDPKWKGAPSSVFIKEAVRLMDEAGYEIGNLDATLILQRPKLSPYKETIRTNLSELLGADPTVVN 695
            +  R+GHGFD+H+     PLI+ G+ VP+E G  AHSDGDV+LH + DAILGA+ L DIG+ FPD+D  +KGA S V ++    L    G+E+GNLD T+I Q PK++P+ E +R  L+  L AD   +N
Sbjct:    1 MKIRIGHGFDVHKFGEARPLILCGVEVPYETGLVAHSDGDVVLHAISDAILGAMALGDIGKHFPDTDAAYKGADSRVLLRHCYALARAKGFELGNLDVTIIAQAPKMAPHIEDMRQVLAADLNADIADIN 130
[Close]
BLAST of EY660988 vs. ExPASy Swiss-Prot
Match: ISPF_SHESM (2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase OS=Shewanella sp. (strain MR-4) GN=ispF PE=3 SV=1)

HSP 1 Score: 132.494 bits (332), Expect = 3.307e-30
Identity = 63/130 (48.46%), Postives = 87/130 (66.92%), Query Frame = 3 
Query:  306 LPFRVGHGFDLHRLEPGYPLIIGGINVPHERGCEAHSDGDVLLHCVVDAILGALGLPDIGQIFPDSDPKWKGAPSSVFIKEAVRLMDEAGYEIGNLDATLILQRPKLSPYKETIRTNLSELLGADPTVVN 695
            +  R+GHGFD+H+     PLI+ G+ VP+E G  AHSDGDV+LH + DAILGA+ L DIG+ FPD+D  +KGA S V ++    L    G+E+GNLD T+I Q PK++P+ E +R  L+  L AD   +N
Sbjct:    1 MKIRIGHGFDVHKFGEARPLILCGVEVPYETGLVAHSDGDVVLHAISDAILGAMALGDIGKHFPDTDAAYKGADSRVLLRHCYALARAKGFELGNLDVTIIAQAPKMAPHIEDMRQVLAADLNADIADIN 130
[Close]
BLAST of EY660988 vs. ExPASy Swiss-Prot
Match: ISPF_SHESA (2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase OS=Shewanella sp. (strain ANA-3) GN=ispF PE=3 SV=1)

HSP 1 Score: 132.494 bits (332), Expect = 3.307e-30
Identity = 63/130 (48.46%), Postives = 87/130 (66.92%), Query Frame = 3 
Query:  306 LPFRVGHGFDLHRLEPGYPLIIGGINVPHERGCEAHSDGDVLLHCVVDAILGALGLPDIGQIFPDSDPKWKGAPSSVFIKEAVRLMDEAGYEIGNLDATLILQRPKLSPYKETIRTNLSELLGADPTVVN 695
            +  R+GHGFD+H+     PLI+ G+ VP+E G  AHSDGDV+LH + DAILGA+ L DIG+ FPD+D  +KGA S V ++    L    G+E+GNLD T+I Q PK++P+ E +R  L+  L AD   +N
Sbjct:    1 MKIRIGHGFDVHKFGEARPLILCGVEVPYETGLVAHSDGDVVLHAISDAILGAMALGDIGKHFPDTDAAYKGADSRVLLRHCYALARAKGFELGNLDVTIIAQAPKMAPHIEDMRQVLAADLNADIADIN 130
[Close]
BLAST of EY660988 vs. ExPASy Swiss-Prot
Match: ISPF_SHEON (2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase OS=Shewanella oneidensis GN=ispF PE=1 SV=1)

HSP 1 Score: 132.494 bits (332), Expect = 3.307e-30
Identity = 63/130 (48.46%), Postives = 87/130 (66.92%), Query Frame = 3 
Query:  306 LPFRVGHGFDLHRLEPGYPLIIGGINVPHERGCEAHSDGDVLLHCVVDAILGALGLPDIGQIFPDSDPKWKGAPSSVFIKEAVRLMDEAGYEIGNLDATLILQRPKLSPYKETIRTNLSELLGADPTVVN 695
            +  R+GHGFD+H+     PLI+ G+ VP+E G  AHSDGDV+LH + DAILGA+ L DIG+ FPD+D  +KGA S V ++    L    G+E+GNLD T+I Q PK++P+ E +R  L+  L AD   +N
Sbjct:    1 MKIRIGHGFDVHKFGEPRPLILCGVEVPYETGLVAHSDGDVVLHAISDAILGAMALGDIGKHFPDTDAAYKGADSRVLLRHCYALAKAKGFELGNLDVTIIAQAPKMAPHIEDMRQVLAADLNADVADIN 130
The following BLAST results are available for this feature:
BLAST of EY660988 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 500
Match NameE-valueIdentityDescription
ISPF_HAEIG2.532e-3049.612-C-methyl-D-erythritol 2,4-cyclodiphosphate synth... [more]
ISPF_EDWI92.532e-3052.992-C-methyl-D-erythritol 2,4-cyclodiphosphate synth... [more]
ISPF_CHRVO2.532e-3047.662-C-methyl-D-erythritol 2,4-cyclodiphosphate synth... [more]
ISPF_ACTSZ2.532e-3048.822-C-methyl-D-erythritol 2,4-cyclodiphosphate synth... [more]
ISPF_SYNP63.307e-3046.772-C-methyl-D-erythritol 2,4-cyclodiphosphate synth... [more]
ISPF_SYNE73.307e-3046.772-C-methyl-D-erythritol 2,4-cyclodiphosphate synth... [more]
ISPF_SHESR3.307e-3048.462-C-methyl-D-erythritol 2,4-cyclodiphosphate synth... [more]
ISPF_SHESM3.307e-3048.462-C-methyl-D-erythritol 2,4-cyclodiphosphate synth... [more]
ISPF_SHESA3.307e-3048.462-C-methyl-D-erythritol 2,4-cyclodiphosphate synth... [more]
ISPF_SHEON3.307e-3048.462-C-methyl-D-erythritol 2,4-cyclodiphosphate synth... [more]

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Properties
Property NameValue
Genbank descriptionCS00-C1-101-028-B12-CT.F Sweet orange leaf, infected with Xylella fastidiosa (stage 1 of 2) Citrus sinensis cDNA, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>EY660988 ID=EY660988; Name=EY660988; organism=Citrus sinensis; type=EST; length=916bp
CCACGCGTCCGGATACATATCGAGCAGACAGCAGAGCACGAACAGCGTCT
CTCCTCTCTTCGTAAAATTAGATGGTGTTGACGATGGCCGCTCAATCATA
CACCACCACTCCTCTCCACAGCAAAATTACGAATAAGCCACTATGTCCTC
CTCCGTTGAGTTTGAAGCCTCGTAGCTTAACCGCCAAACATCTCCGCACA
ACACAGTCAACGTCGCTGCCTCGTATTTCAGTTTCAGCTGCAGCCATTTC
TTCCATCGAAGTTAAAGAGTCATCGGCTTCGATCCAACCGTCCAAATCAA
AATCACTGCCTTTTCGTGTGGGCCACGGGTTTGATCTTCATAGATTGGAG
CCTGGCTACCCTCTGATCATCGGTGGAATCAATGTTCCCCACGAGAGAGG
CTGCGAGGCTCACTCTGACGGTGATGTTTTGCTTCACTGTGTTGTGGATG
CAATTTTGGGAGCATTGGGTCTTCCTGATATTGGTCAGATATTTCCTGAT
TCTGATCCTAAGTGGAAGGGTGCACCATCCTCTGTTTTTATCAAAGAAGC
TGTGAGACTCATGGATGAGGCCGGCTATGAGATTGGCAACTTGGATGCCA
CGCTGATTCTTCAAAGACCAAAATTGAGCCCATACAAGGAGACTATCCGA
ACCAACTTGTCTGAACTGCTAGGAGCAGACCCCACCGTTGTAAATCTGAA
AGCGAAACTCATGAAAAAGTTGATAGCCTTGGTGAAAATCGAAGTATTGC
AGCTCACACTGTGATCCTTCTCATGAAGAAAGTAAATAAACGATTTGGGA
ATTTGTCTAATGTAGGCAGTGGATCCTATTATCNATGGTGNATTTGTATT
CTGGCTACAAGACTATCCACTGGTATAGATTCCAATGATAGTTTNNGTTC
TACTTGAACATGGCTC
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