EY660661 | Citrus Genome Database

Overview


Name	EY660661
Unique Name	EY660661
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	886

Libraries


Library Name	Type
Sweet orange leaf, infected with Xylella fastidiosa (stage 1 of 2)	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Homology

[Close]

BLAST of EY660661 vs. ExPASy Swiss-Prot
Match: ENO_DESPS (Enolase OS=Desulfotalea psychrophila GN=eno PE=3 SV=1)

HSP 1 Score: 180.644 bits (457), Expect = 4.622e-45
Identity = 105/192 (54.69%), Postives = 129/192 (67.19%), Query Frame = 1

Query:   76 MAITITAVKARQIFDSRGNPTVEVDVTTSDGHVARAAVPSGASTGIYEALELRDG-GSDYLGKGVSKAVSNVNAIIGPALAGKDPTEQTAIDNYMVQQLDGTVNEWGWCKQKLGANAILAVSLAVCKAGAHVKKIPLYKHIAELSGNKNLVLPVPAFNVINGGSHAGNKLAMQEFMILPVGASSFKKP*RWV 648
            M+  I+AV A ++ DSRGNPTV V VT  +G    A+VPSGASTG  EA+ELRDG  S YLGKGV KAV NV  II PA+ GK  T Q AIDN M+ +LDGT       K  LGANAIL VS+AV +AGA   ++PLY++   L G     +PVPA N+INGG HA N + +QEFM +PVGA SF +  R++
Sbjct:    1 MSDIISAVDAMEVLDSRGNPTVRVFVTLDNGTTCAASVPSGASTGENEAIELRDGDASRYLGKGVLKAVDNVKNIIAPAVIGKKITHQAAIDNLMI-ELDGTET-----KSSLGANAILGVSMAVARAGARSSRLPLYQY---LGGPGARRIPVPAMNIINGGEHADNSVDIQEFMAVPVGAPSFGEGLRYI 183

HSP 2 Score: 22.7126 bits (47), Expect = 4.622e-45
Identity = 9/12 (75.00%), Postives = 10/12 (83.33%), Query Frame = 2

Query:  701 ATNGGDESGFAP 736
            AT+ GDE GFAP
Sbjct:  200 ATSVGDEGGFAP 211

[Close]

BLAST of EY660661 vs. ExPASy Swiss-Prot
Match: ENO_DESVV (Enolase OS=Desulfovibrio vulgaris subsp. vulgaris (strain DP4) GN=eno PE=3 SV=1)

HSP 1 Score: 175.637 bits (444), Expect = 5.555e-45
Identity = 99/183 (54.10%), Postives = 124/183 (67.76%), Query Frame = 1

Query:   85 TITAVKARQIFDSRGNPTVEVDVTTSDGHVARAAVPSGASTGIYEALELRDGGSD-YLGKGVSKAVSNVNAIIGPALAGKDPTEQTAIDNYMVQQLDGTVNEWGWCKQKLGANAILAVSLAVCKAGAHVKKIPLYKHIAELSGNKNLVLPVPAFNVINGGSHAGNKLAMQEFMILPVGASSFK 630
            TI +V AR+I DSRGNPT+EV+V+   GH  RAAVPSGASTG  EALELRDG    Y GKGV KAV NV   I  A+ G D   Q  +DN ++  LDGT N     K++LGANA+L VSLA  +A +    +PLY++   L G    VLPVP  N+INGG+HA N L +QEFMI+P+GA++F+
Sbjct:    3 TIVSVWAREILDSRGNPTIEVEVSLESGHSGRAAVPSGASTGTREALELRDGDKGRYKGKGVEKAVDNVMGEIAEAVIGLDALRQVQLDNTLL-DLDGTDN-----KERLGANAMLGVSLATARAASSFLGLPLYQY---LGGVNAKVLPVPLMNIINGGAHAPNNLDIQEFMIMPIGAATFR 176

HSP 2 Score: 26.9498 bits (58), Expect = 5.555e-45
Identity = 9/19 (47.37%), Postives = 16/19 (84.21%), Query Frame = 3

Query:  624 FQEAMKMGVEVYHHLKAVI 680
            F++A++MG E +H LKA++
Sbjct:  175 FRDALRMGAETFHTLKALL 193

HSP 3 Score: 21.1718 bits (43), Expect = 5.555e-45
Identity = 8/11 (72.73%), Postives = 9/11 (81.82%), Query Frame = 2

Query:  704 TNGGDESGFAP 736
            T+ GDE GFAP
Sbjct:  200 TSVGDEGGFAP 210

[Close]

BLAST of EY660661 vs. ExPASy Swiss-Prot
Match: ENO_DESVH (Enolase OS=Desulfovibrio vulgaris (strain Hildenborough / ATCC 29579 / NCIMB 8303) GN=eno PE=3 SV=1)

HSP 1 Score: 175.637 bits (444), Expect = 5.555e-45
Identity = 99/183 (54.10%), Postives = 124/183 (67.76%), Query Frame = 1

Query:   85 TITAVKARQIFDSRGNPTVEVDVTTSDGHVARAAVPSGASTGIYEALELRDGGSD-YLGKGVSKAVSNVNAIIGPALAGKDPTEQTAIDNYMVQQLDGTVNEWGWCKQKLGANAILAVSLAVCKAGAHVKKIPLYKHIAELSGNKNLVLPVPAFNVINGGSHAGNKLAMQEFMILPVGASSFK 630
            TI +V AR+I DSRGNPT+EV+V+   GH  RAAVPSGASTG  EALELRDG    Y GKGV KAV NV   I  A+ G D   Q  +DN ++  LDGT N     K++LGANA+L VSLA  +A +    +PLY++   L G    VLPVP  N+INGG+HA N L +QEFMI+P+GA++F+
Sbjct:    3 TIVSVWAREILDSRGNPTIEVEVSLESGHSGRAAVPSGASTGTREALELRDGDKGRYKGKGVEKAVDNVMGEIAEAVIGLDALRQVQLDNTLL-DLDGTDN-----KERLGANAMLGVSLATARAASSFLGLPLYQY---LGGVNAKVLPVPLMNIINGGAHAPNNLDIQEFMIMPIGAATFR 176

HSP 2 Score: 26.9498 bits (58), Expect = 5.555e-45
Identity = 9/19 (47.37%), Postives = 16/19 (84.21%), Query Frame = 3

Query:  624 FQEAMKMGVEVYHHLKAVI 680
            F++A++MG E +H LKA++
Sbjct:  175 FRDALRMGAETFHTLKALL 193

HSP 3 Score: 21.1718 bits (43), Expect = 5.555e-45
Identity = 8/11 (72.73%), Postives = 9/11 (81.82%), Query Frame = 2

Query:  704 TNGGDESGFAP 736
            T+ GDE GFAP
Sbjct:  200 TSVGDEGGFAP 210

[Close]

BLAST of EY660661 vs. ExPASy Swiss-Prot
Match: ENO_PERMH (Enolase OS=Persephonella marina (strain DSM 14350 / EX-H1) GN=eno PE=3 SV=1)

HSP 1 Score: 167.162 bits (422), Expect = 5.557e-45
Identity = 98/183 (53.55%), Postives = 118/183 (64.48%), Query Frame = 1

Query:   88 ITAVKARQIFDSRGNPTVEVDVTTSDGHVARAAVPSGASTGIYEALELRDGGSD-YLGKGVSKAVSNVNAIIGPALAGKDPTEQTAIDNYMVQQLDGTVNEWGWCKQKLGANAILAVSLAVCKAGAHVKKIPLYKHIAELSGNKNLVLPVPAFNVINGGSHAGNKLAMQEFMILPVGASSFKK 633
            I  ++ R++ DSRGNPTVE +V    G VA A VPSGASTG  EA+ELRDG  + + GKGV KAV N+N  I   L G++  +Q  ID  M+ +LDGT N     K  LGANAILAVSLAV +A A    IPLY++   L G    VLPVP  NVINGG+HA N L  QEFMI+PV    FK+
Sbjct:    4 IVDIRGREVLDSRGNPTVEAEVVLESGVVATALVPSGASTGETEAVELRDGDKNRFKGKGVLKAVDNINTKIADLLIGENALDQVRIDRLML-ELDGTEN-----KSNLGANAILAVSLAVARAAAMELDIPLYRY---LGGTNAKVLPVPLMNVINGGAHADNNLDFQEFMIVPVFGGRFKE 177

HSP 2 Score: 30.0314 bits (66), Expect = 5.557e-45
Identity = 11/20 (55.00%), Postives = 17/20 (85.00%), Query Frame = 3

Query:  624 FQEAMKMGVEVYHHLKAVIK 683
            F+EA++ GVE++H LK V+K
Sbjct:  175 FKEALRCGVEIFHTLKTVLK 194

HSP 3 Score: 26.5646 bits (57), Expect = 5.557e-45
Identity = 11/22 (50.00%), Postives = 15/22 (68.18%), Query Frame = 2

Query:  701 ATNGGDESGFAPIFXRTRKDLN 766
            +TN GDE GFAP    T++ L+
Sbjct:  199 STNVGDEGGFAPALNSTKEALD 220

[Close]

BLAST of EY660661 vs. ExPASy Swiss-Prot
Match: ENO_HYPNA (Enolase OS=Hyphomonas neptunium (strain ATCC 15444) GN=eno PE=3 SV=1)

HSP 1 Score: 176.407 bits (446), Expect = 5.558e-45
Identity = 102/183 (55.74%), Postives = 119/183 (65.03%), Query Frame = 1

Query:   88 ITAVKARQIFDSRGNPTVEVDVTTSDGHVARAAVPSGASTGIYEALELRDGG-SDYLGKGVSKAVSNVNAIIGPALAGKDPTEQTAIDNYMVQQLDGTVNEWGWCKQKLGANAILAVSLAVCKAGAHVKKIPLYKHIAELSGNKNLVLPVPAFNVINGGSHAGNKLAMQEFMILPVGASSFKK 633
            I  + AR+I DSRGNPT+EVDVT  DG   RAAVPSGASTG YEA E RDG  + Y GKGV KAV  VN  I   LAG D TEQ  +D  M+  LDGT N     K +LGANAIL VSLAV KA A    +PLY++   L G    +LP P  N+INGG+HA N + +QEFMI+PV A S  +
Sbjct:    4 IIDIHAREILDSRGNPTIEVDVTLDDGSTGRAAVPSGASTGAYEAHEQRDGDKARYNGKGVLKAVDAVNGEICNELAGMDATEQRLVDTLMI-DLDGTEN-----KSRLGANAILGVSLAVAKAAAESSALPLYRY---LGGANARILPTPMMNIINGGAHADNPVDIQEFMIMPVSAESISE 177

HSP 2 Score: 24.2534 bits (51), Expect = 5.558e-45
Identity = 9/17 (52.94%), Postives = 13/17 (76.47%), Query Frame = 3

Query:  630 EAMKMGVEVYHHLKAVI 680
            EA++MG EV+H LK  +
Sbjct:  177 EAVRMGAEVFHALKKTL 193

HSP 3 Score: 23.0978 bits (48), Expect = 5.558e-45
Identity = 9/11 (81.82%), Postives = 9/11 (81.82%), Query Frame = 2

Query:  704 TNGGDESGFAP 736
            TN GDE GFAP
Sbjct:  200 TNVGDEGGFAP 210

[Close]

BLAST of EY660661 vs. ExPASy Swiss-Prot
Match: ENO_DESMR (Enolase OS=Desulfovibrio magneticus (strain ATCC 700980 / DSM 13731 / RS-1) GN=eno PE=3 SV=1)

HSP 1 Score: 181.415 bits (459), Expect = 5.911e-45
Identity = 103/182 (56.59%), Postives = 125/182 (68.68%), Query Frame = 1

Query:   85 TITAVKARQIFDSRGNPTVEVDVTTSDGHVARAAVPSGASTGIYEALELRD-GGSDYLGKGVSKAVSNVNAIIGPALAGKDPTEQTAIDNYMVQQLDGTVNEWGWCKQKLGANAILAVSLAVCKAGAHVKKIPLYKHIAELSGNKNLVLPVPAFNVINGGSHAGNKLAMQEFMILPVGASSF 627
            TI AV AR+I DSRGNPTVEV+VT   G   RAAVPSGASTG  EALE+RD   S Y GKGV+KAV NV   +   + G D  +Q AIDN M+   DGT N     K +LGANAIL VSLAVC+A ++   +PLY++I    G  + VLPVP  N+INGG+HA N L +QEFMI+P+GA +F
Sbjct:    3 TIAAVWAREILDSRGNPTVEVEVTLESGASGRAAVPSGASTGSREALEMRDQDASRYAGKGVTKAVENVQGELADTVIGMDALQQVAIDNLMI-DTDGTEN-----KSRLGANAILGVSLAVCRAASNFLGLPLYQYI---GGINSKVLPVPMMNIINGGAHAPNNLDIQEFMIIPLGARTF 175

[Close]

BLAST of EY660661 vs. ExPASy Swiss-Prot
Match: ENO_CHLT3 (Enolase OS=Chloroherpeton thalassium (strain ATCC 35110 / GB-78) GN=eno PE=3 SV=1)

HSP 1 Score: 181.415 bits (459), Expect = 5.911e-45
Identity = 106/181 (58.56%), Postives = 124/181 (68.51%), Query Frame = 1

Query:   88 ITAVKARQIFDSRGNPTVEVDVTTSDGHVARAAVPSGASTGIYEALELRDGGSD-YLGKGVSKAVSNVNAIIGPALAGKDPTEQTAIDNYMVQQLDGTVNEWGWCKQKLGANAILAVSLAVCKAGAHVKKIPLYKHIAELSGNKNLVLPVPAFNVINGGSHAGNKLAMQEFMILPVGASSF 627
            I  + ARQI DSRGNPTVEVDV T D    RAAVPSGASTG++EA+ELRDG  + YLGK V KAV NVN II   L+G   TEQ+ IDN ++Q LDGT N     K KLGANAIL VSLA  KAGA    +PLY++I    G     LPVP  NV+NGG+HA N +  QEFMI+P+G  +F
Sbjct:    4 IQRIFARQILDSRGNPTVEVDVFT-DSSFGRAAVPSGASTGVHEAVELRDGDKEFYLGKSVMKAVENVNTIINDELSGMLVTEQSEIDNALLQ-LDGTPN-----KSKLGANAILGVSLACAKAGADYSGLPLYRYIG---GTLATQLPVPMMNVLNGGAHADNNVDFQEFMIMPIGFDTF 174

[Close]

BLAST of EY660661 vs. ExPASy Swiss-Prot
Match: ENO_MYCGA (Enolase OS=Mycoplasma gallisepticum GN=eno PE=3 SV=1)

HSP 1 Score: 179.489 bits (454), Expect = 6.016e-45
Identity = 101/187 (54.01%), Postives = 126/187 (67.38%), Query Frame = 1

Query:   82 ITITAVKARQIFDSRGNPTVEVDVTTSDGHVARAAVPSGASTGIYEALELRDGGSDYLGKGVSKAVSNVNAIIGPALAGKDPTEQTAIDNYMVQQLDGTVNEWGWCKQKLGANAILAVSLAVCKAGAHVKKIPLYKHIAELSG---NKNLVLPVPAFNVINGGSHAGNKLAMQEFMILPVGASSFKK 633
            + I +V A Q FDSRG PTV  +V  +DG    + V SGASTG  EALELRDGG+ Y GKGV+KAV+N+N  IGP + G D T QT ID +M+ +LDGT       K KLGANAILAVS+AVC+A A    +PLY++IA+        + +LPVP  NVINGG+HA N +  QEFMI+PVGA +  K
Sbjct:   13 LEIKSVFAYQAFDSRGFPTVACEVVLNDGSKGLSMVSSGASTGEKEALELRDGGTKYHGKGVTKAVNNINKKIGPKILGVDATLQTQIDEFMI-ELDGTKT-----KAKLGANAILAVSMAVCRAAAKSLNLPLYQYIAKKVAKVKGADFILPVPMLNVINGGAHADNTIDFQEFMIMPVGAKTMAK 193

HSP 2 Score: 23.483 bits (49), Expect = 6.016e-45
Identity = 9/11 (81.82%), Postives = 9/11 (81.82%), Query Frame = 2

Query:  704 TNGGDESGFAP 736
            TN GDE GFAP
Sbjct:  216 TNKGDEGGFAP 226

[Close]

BLAST of EY660661 vs. ExPASy Swiss-Prot
Match: ENO_YERPY (Enolase OS=Yersinia pseudotuberculosis serotype O:3 (strain YPIII) GN=eno PE=3 SV=1)

HSP 1 Score: 175.637 bits (444), Expect = 6.022e-45
Identity = 103/184 (55.98%), Postives = 121/184 (65.76%), Query Frame = 1

Query:   88 ITAVKARQIFDSRGNPTVEVDVTTSDGHVARAAVPSGASTGIYEALELRDGG-SDYLGKGVSKAVSNVNAIIGPALAGKDPTEQTAIDNYMVQQLDGTVNEWGWCKQKLGANAILAVSLAVCKAGAHVKKIPLYKHIAELSGNK-NLVLPVPAFNVINGGSHAGNKLAMQEFMILPVGASSFKK 633
            I  V  R+I DSRGNPTVE +V    G V  AA PSGASTG  EALELRDG  S +LGKGV KAV+ VN  I  A+ GKD  +Q  ID  M+  LDGT N     K + GANAILAVSLA  KA A  K +PLY+HIAEL+G      +P+P  N+INGG HA N + +QEFMI PVGA + K+
Sbjct:    4 IVKVIGREIIDSRGNPTVEAEVHLEGGFVGLAAAPSGASTGSREALELRDGDKSRFLGKGVLKAVAAVNGPIAQAVIGKDAKDQANIDKIMI-DLDGTEN-----KSQFGANAILAVSLAAAKAAAASKGMPLYEHIAELNGTPGKFSMPLPMMNIINGGEHADNNVDIQEFMIQPVGAKTLKE 181

HSP 2 Score: 27.335 bits (59), Expect = 6.022e-45
Identity = 10/19 (52.63%), Postives = 16/19 (84.21%), Query Frame = 3

Query:  627 QEAMKMGVEVYHHLKAVIK 683
            +EA+++G EV+HHL  V+K
Sbjct:  180 KEAVRIGSEVFHHLAKVLK 198

[Close]

BLAST of EY660661 vs. ExPASy Swiss-Prot
Match: ENO_YERPS (Enolase OS=Yersinia pseudotuberculosis GN=eno PE=3 SV=1)

HSP 1 Score: 175.637 bits (444), Expect = 6.022e-45
Identity = 103/184 (55.98%), Postives = 121/184 (65.76%), Query Frame = 1

Query:   88 ITAVKARQIFDSRGNPTVEVDVTTSDGHVARAAVPSGASTGIYEALELRDGG-SDYLGKGVSKAVSNVNAIIGPALAGKDPTEQTAIDNYMVQQLDGTVNEWGWCKQKLGANAILAVSLAVCKAGAHVKKIPLYKHIAELSGNK-NLVLPVPAFNVINGGSHAGNKLAMQEFMILPVGASSFKK 633
            I  V  R+I DSRGNPTVE +V    G V  AA PSGASTG  EALELRDG  S +LGKGV KAV+ VN  I  A+ GKD  +Q  ID  M+  LDGT N     K + GANAILAVSLA  KA A  K +PLY+HIAEL+G      +P+P  N+INGG HA N + +QEFMI PVGA + K+
Sbjct:    4 IVKVIGREIIDSRGNPTVEAEVHLEGGFVGLAAAPSGASTGSREALELRDGDKSRFLGKGVLKAVAAVNGPIAQAVIGKDAKDQANIDKIMI-DLDGTEN-----KSQFGANAILAVSLAAAKAAAASKGMPLYEHIAELNGTPGKFSMPLPMMNIINGGEHADNNVDIQEFMIQPVGAKTLKE 181

HSP 2 Score: 27.335 bits (59), Expect = 6.022e-45
Identity = 10/19 (52.63%), Postives = 16/19 (84.21%), Query Frame = 3

Query:  627 QEAMKMGVEVYHHLKAVIK 683
            +EA+++G EV+HHL  V+K
Sbjct:  180 KEAVRIGSEVFHHLAKVLK 198

The following BLAST results are available for this feature:

BLAST of EY660661 vs. ExPASy Swiss-Prot

BLAST of EY660661 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 500


Match Name	E-value	Identity	Description
ENO_DESPS	4.622e-45	54.69	Enolase OS=Desulfotalea psychrophila GN=eno PE=3 S...	[more]
ENO_DESVV	5.555e-45	54.10	Enolase OS=Desulfovibrio vulgaris subsp. vulgaris ...	[more]
ENO_DESVH	5.555e-45	54.10	Enolase OS=Desulfovibrio vulgaris (strain Hildenbo...	[more]
ENO_PERMH	5.557e-45	53.55	Enolase OS=Persephonella marina (strain DSM 14350 ...	[more]
ENO_HYPNA	5.558e-45	55.74	Enolase OS=Hyphomonas neptunium (strain ATCC 15444...	[more]
ENO_DESMR	5.911e-45	56.59	Enolase OS=Desulfovibrio magneticus (strain ATCC 7...	[more]
ENO_CHLT3	5.911e-45	58.56	Enolase OS=Chloroherpeton thalassium (strain ATCC ...	[more]
ENO_MYCGA	6.016e-45	54.01	Enolase OS=Mycoplasma gallisepticum GN=eno PE=3 SV...	[more]
ENO_YERPY	6.022e-45	55.98	Enolase OS=Yersinia pseudotuberculosis serotype O:...	[more]
ENO_YERPS	6.022e-45	55.98	Enolase OS=Yersinia pseudotuberculosis GN=eno PE=3...	[more]

Pages

Properties


Property Name	Value
Genbank description	CS00-C1-101-024-F03-CT.F Sweet orange leaf, infected with Xylella fastidiosa (stage 1 of 2) Citrus sinensis cDNA, mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>EY660661 ID=EY660661; Name=EY660661; organism=Citrus sinensis; type=EST; length=886bp
CCACGCGTCCGCGGACGCGTGGGCAGACTCTCTCTCTCGTTCTAGATCTT
TCTTCGCTTTTCCAGCTTTCAGAAAATGGCAATAACCATCACTGCCGTCA
AAGCCAGGCAGATCTTTGACAGCCGTGGAAATCCAACGGTGGAGGTCGAC
GTGACAACATCAGATGGCCACGTGGCTAGAGCTGCTGTTCCAAGCGGTGC
ATCCACCGGAATTTATGAGGCGCTTGAACTAAGAGACGGAGGCTCTGATT
ACCTAGGCAAGGGTGTATCGAAGGCTGTTAGCAATGTCAACGCCATCATT
GGCCCTGCACTCGCTGGCAAGGACCCAACTGAGCAGACTGCTATTGACAA
CTATATGGTACAACAACTTGATGGAACTGTAAATGAGTGGGGTTGGTGCA
AACAGAAGCTTGGAGCAAACGCCATTTTGGCTGTCTCCCTAGCTGTCTGC
AAGGCTGGTGCACATGTCAAGAAGATTCCTCTTTACAAGCATATTGCCGA
ACTCTCTGGTAACAAGAATTTGGTGTTGCCAGTGCCAGCATTCAATGTCA
TTAATGGTGGATCACATGCTGGAAACAAACTTGCTATGCAGGAGTTTATG
ATTCTTCCTGTAGGAGCTTCCTCTTTCAAGAAGCCATGAAGATGGGTGTA
GAAGTATATCACCATCTGAAGGCTGTGATTAAAAAGAATACGGNCCAGAT
GCTACCAATGGTGGGGATGAAAGCGGTTTTGCTCCCATATTCANGAGAAC
AAGGAAGGACTTGAATTGCTCACACAGCCATTGCTAAGCCGGTTACACAA
GCAAGTGTCATTGGATGGATGTGCTGCTCTGAATTCATGGTCAAATAGAC
TATGATCTGACTCCAGGAAAAAACATGATGGTCACA