EY660263 | Citrus Genome Database

Overview


Name	EY660263
Unique Name	EY660263
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	918

Libraries


Library Name	Type
Sweet orange leaf, infected with Xylella fastidiosa (stage 1 of 2)	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Homology

[Close]

BLAST of EY660263 vs. ExPASy Swiss-Prot
Match: PHSG_YEAST (Glycogen phosphorylase OS=Saccharomyces cerevisiae GN=GPH1 PE=1 SV=3)

HSP 1 Score: 63.929 bits (154), Expect = 3.512e-16
Identity = 30/53 (56.60%), Postives = 42/53 (79.25%), Query Frame = 3

Query:  351 PASELSQHISTAWMEARATTDVKFAKNGCMVVGTLDGANGEIREQVGED-IFL 506
            PAS+LS+HISTA  EA  T+++KF  NG +++GT+DGAN EI  ++GED +FL
Sbjct:  729 PASDLSEHISTAGTEASGTSNMKFVMNGGLIIGTVDGANVEITREIGEDNVFL 781

HSP 2 Score: 33.113 bits (74), Expect = 3.512e-16
Identity = 24/66 (36.36%), Postives = 34/66 (51.52%), Query Frame = 1

Query:   64 LKSIFELERQLMTI*RIVDTYKEMLEM----SAVKRNAKFVPR-VCIFGGKAFASYVQANTIGKFI 246
            +K I E +RQ + +  I+  Y  M  M    ++++  AK  PR V IFGGK+   Y  A  I K I
Sbjct:  629 VKRIHEYKRQQLNVFGIIYRYLAMKNMLKNGASIEEVAKKYPRKVSIFGGKSAPGYYMAKLIIKLI 694

HSP 3 Score: 28.8758 bits (63), Expect = 3.512e-16
Identity = 13/22 (59.09%), Postives = 17/22 (77.27%), Query Frame = 2

Query:  284 IGDLLKGIFVPDYNVREAELLI 349
            I  LLK +FV DYNV +AE++I
Sbjct:  707 IEHLLKVVFVADYNVSKAEIII 728

[Close]

BLAST of EY660263 vs. ExPASy Swiss-Prot
Match: PHS1_DICDI (Glycogen phosphorylase 1 OS=Dictyostelium discoideum GN=glpV PE=1 SV=3)

HSP 1 Score: 62.3882 bits (150), Expect = 3.520e-16
Identity = 29/61 (47.54%), Postives = 43/61 (70.49%), Query Frame = 3

Query:  351 PASELSQHISTAWMEARATTDVKFAKNGCMVVGTLDGANGEIREQVGEDIFLLLCDRAYEI 533
            PAS+++Q ISTA  EA  T+++KF  NG +++GTLDGAN EI E+VG++   +   R  E+
Sbjct:  675 PASDINQQISTAGTEASGTSNMKFTMNGSLIIGTLDGANVEIAEEVGQENMFIFGLRTSEV 735

HSP 2 Score: 41.5874 bits (96), Expect = 3.520e-16
Identity = 26/61 (42.62%), Postives = 34/61 (55.74%), Query Frame = 1

Query:   64 LKSIFELERQLMTI*RIVDTYKEMLEMSAVKRNAKFVPRVCIFGGKAFASYVQANTIGKFI 246
            +K I E +RQL+ I  ++  Y  + +MS  K  A+ VPRV IF GKA   YV A    K I
Sbjct:  581 IKRIHEYKRQLLNILSVIYRYLSIKKMSP-KDRAQVVPRVVIFAGKAAPGYVMAKRHIKLI 640

HSP 3 Score: 21.9422 bits (45), Expect = 3.520e-16
Identity = 7/23 (30.43%), Postives = 16/23 (69.57%), Query Frame = 2

Query:  281 KIGDLLKGIFVPDYNVREAELLI 349
            ++   LK +F+ +YNV  A++++
Sbjct:  652 EVDQYLKVVFIANYNVSIAQVIV 674

[Close]

BLAST of EY660263 vs. ExPASy Swiss-Prot
Match: PHSG_PASMU (Glycogen phosphorylase OS=Pasteurella multocida GN=glgP PE=3 SV=1)

HSP 1 Score: 57.3806 bits (137), Expect = 5.690e-16
Identity = 26/49 (53.06%), Postives = 37/49 (75.51%), Query Frame = 3

Query:  351 PASELSQHISTAWMEARATTDVKFAKNGCMVVGTLDGANGEIREQVGED 497
            PA+++S+ IS A  EA  T+++KFA NG + +GTLDGAN EI + VG+D
Sbjct:  645 PAADISEQISLAGTEASGTSNMKFALNGALTIGTLDGANVEILDNVGQD 693

HSP 2 Score: 36.1946 bits (82), Expect = 5.690e-16
Identity = 22/63 (34.92%), Postives = 31/63 (49.21%), Query Frame = 1

Query:   64 LKSIFELERQLMTI*RIVDTYKEMLEMSAVKRNAKFVPRVCIFGGKAFASYVQANTIGKFITD 252
            +K I E +RQ++ +  I+  Y  MLE         +VPRV I  GKA ++Y  A      I D
Sbjct:  554 VKRIHEYKRQILNVLHIIARYNAMLE----NPEKDWVPRVFILAGKAASAYYAAKQTINLIND 612

HSP 3 Score: 25.7942 bits (55), Expect = 5.690e-16
Identity = 17/74 (22.97%), Postives = 30/74 (40.54%), Query Frame = 1

Query:  610 DQVVLGVTAMMNLWDHWNEMNALAVHIIANECTDFPSYLECQDKVFEACCHLNRWTRMTIMNTSCSLKMNTNRT 831
            DQ++ G  +  +   +   + +L  H       DF SY++ Q  V       N W   T+ N       +++RT
Sbjct:  730 DQIISGRFSPTDANRYHQLLQSLQYHDYYQAFADFRSYVDMQQNVDAKYQDQNAWIDSTLQNIVNMSYFSSDRT 803

HSP 4 Score: 25.0238 bits (53), Expect = 5.690e-16
Identity = 10/18 (55.56%), Postives = 15/18 (83.33%), Query Frame = 2

Query:  296 LKGIFVPDYNVREAELLI 349
            LK +F+P+Y+V  AEL+I
Sbjct:  627 LKVVFIPNYSVSLAELII 644

[Close]

BLAST of EY660263 vs. ExPASy Swiss-Prot
Match: PHSG_SYNY3 (Glycogen phosphorylase OS=Synechocystis sp. (strain PCC 6803) GN=glgP PE=3 SV=1)

HSP 1 Score: 62.7734 bits (151), Expect = 2.140e-15
Identity = 30/53 (56.60%), Postives = 40/53 (75.47%), Query Frame = 3

Query:  351 PASELSQHISTAWMEARATTDVKFAKNGCMVVGTLDGANGEIREQVGEDIFLL 509
            PA++LS+ ISTA  EA  T ++KF+ NG + +GTLDGAN EIRE+VG + F L
Sbjct:  657 PAADLSEQISTAGKEASGTGNMKFSMNGALTIGTLDGANIEIREEVGAENFFL 709

HSP 2 Score: 31.5722 bits (70), Expect = 2.140e-15
Identity = 20/61 (32.79%), Postives = 28/61 (45.90%), Query Frame = 1

Query:   64 LKSIFELERQLMTI*RIVDTYKEMLEMSAVKRNAKFVPRVCIFGGKAFASYVQANTIGKFI 246
            +K I E +RQ + I  ++  Y ++        N    PR  I+GGKA   Y  A  I K I
Sbjct:  566 VKRIHEYKRQHLNILHVIHLYLQIKN----NPNLDVTPRTFIYGGKAAPGYFTAKLIIKLI 622

HSP 3 Score: 28.8758 bits (63), Expect = 2.140e-15
Identity = 12/16 (75.00%), Postives = 14/16 (87.50%), Query Frame = 2

Query:  284 IGDLLKGIFVPDYNVR 331
            IGD LK IF+PDYNV+
Sbjct:  635 IGDRLKVIFLPDYNVK 650

[Close]

BLAST of EY660263 vs. ExPASy Swiss-Prot
Match: PHSG_CHLTR (Glycogen phosphorylase OS=Chlamydia trachomatis GN=glgP PE=3 SV=1)

HSP 1 Score: 59.3066 bits (142), Expect = 6.364e-15
Identity = 31/64 (48.44%), Postives = 45/64 (70.31%), Query Frame = 3

Query:  351 PASELSQHISTAWMEARATTDVKFAKNGCMVVGTLDGANGEIREQVGED---IFLLLCDRAYEI 533
            PA++LS+ ISTA MEA  T ++KFA NG + +GT+DGAN E+ E +G++   IF LL +   E+
Sbjct:  640 PATDLSEQISTAGMEASGTGNMKFALNGALTIGTMDGANIEMAEHIGKEHMFIFGLLEEEISEL 703

HSP 2 Score: 42.743 bits (99), Expect = 6.364e-15
Identity = 28/73 (38.36%), Postives = 36/73 (49.32%), Query Frame = 1

Query:   64 LKSIFELERQLMTI*RIVDTYKEMLEMSAVKRNAKFVPRVCIFGGKAFASYVQANTIGKFITDGGATVCLGPE 282
            +K I E +RQLM I R++  Y E+   S      + VP   IFGGKA   Y  A  I K I +  A V   P+
Sbjct:  550 IKRIHEYKRQLMNILRVIYFYNEIRNGSG-----EIVPTTVIFGGKAAPGYAMAKLIIKLINNVAAVVNNDPK 617

[Close]

BLAST of EY660263 vs. ExPASy Swiss-Prot
Match: PHSM_ECOLI (Maltodextrin phosphorylase OS=Escherichia coli (strain K12) GN=malP PE=1 SV=7)

HSP 1 Score: 58.151 bits (139), Expect = 7.777e-15
Identity = 28/49 (57.14%), Postives = 37/49 (75.51%), Query Frame = 3

Query:  351 PASELSQHISTAWMEARATTDVKFAKNGCMVVGTLDGANGEIREQVGED 497
            PA+++S+ ISTA  EA  T ++K A NG + VGTLDGAN EI E+VGE+
Sbjct:  624 PAADISEQISTAGKEASGTGNMKLALNGALTVGTLDGANVEIAEKVGEE 672

HSP 2 Score: 34.2686 bits (77), Expect = 7.777e-15
Identity = 21/57 (36.84%), Postives = 29/57 (50.88%), Query Frame = 1

Query:   64 LKSIFELERQLMTI*RIVDTYKEMLEMSAVKRNAKFVPRVCIFGGKAFASYVQANTI 234
            +K + E +RQ + +  I+  YKE+ E     R    VPRV +FG KA   Y  A  I
Sbjct:  533 IKRLHEYKRQHLNLLHILALYKEIRENPQADR----VPRVFLFGAKAAPGYYLAKNI 585

HSP 3 Score: 28.8758 bits (63), Expect = 7.777e-15
Identity = 13/22 (59.09%), Postives = 16/22 (72.73%), Query Frame = 2

Query:  284 IGDLLKGIFVPDYNVREAELLI 349
            +GD LK +F+PDY V  AE LI
Sbjct:  602 VGDKLKVVFLPDYCVSAAEKLI 623

[Close]

BLAST of EY660263 vs. ExPASy Swiss-Prot
Match: PHSG_CHLMU (Glycogen phosphorylase OS=Chlamydia muridarum GN=glgP PE=3 SV=1)

HSP 1 Score: 58.5362 bits (140), Expect = 8.268e-15
Identity = 26/49 (53.06%), Postives = 38/49 (77.55%), Query Frame = 3

Query:  351 PASELSQHISTAWMEARATTDVKFAKNGCMVVGTLDGANGEIREQVGED 497
            PA++LS+ ISTA MEA  T ++KFA NG + +GT+DGAN E+ E +G++
Sbjct:  640 PATDLSEQISTAGMEASGTGNMKFALNGALTIGTMDGANIEMAEHIGKE 688

HSP 2 Score: 43.1282 bits (100), Expect = 8.268e-15
Identity = 30/84 (35.71%), Postives = 41/84 (48.81%), Query Frame = 1

Query:   64 LKSIFELERQLMTI*RIVDTYKEMLEMSAVKRNAKFVPRVCIFGGKAFASYVQANTIGKFITDGGATVCLGPEDR*LIEGNFCP 315
            +K I E +RQLM I R++  Y E+   S      + VP   IFGGKA   Y  A  I K I +    V   P+ + L++  F P
Sbjct:  550 IKRIHEYKRQLMNILRVIYFYNEIRNGST-----EIVPTTVIFGGKAAPGYAMAKLIIKLINNVAHIVNNDPKAKDLLKVVFWP 628

[Close]

BLAST of EY660263 vs. ExPASy Swiss-Prot
Match: PYGL_SHEEP (Glycogen phosphorylase, liver form OS=Ovis aries GN=PYGL PE=2 SV=3)

HSP 1 Score: 58.9214 bits (141), Expect = 1.907e-13
Identity = 27/62 (43.55%), Postives = 42/62 (67.74%), Query Frame = 3

Query:  351 PASELSQHISTAWMEARATTDVKFAKNGCMVVGTLDGANGEIREQVGEDIFLLLCDRAYEIA 536
            PA++LS+ ISTA  EA  T ++KF +NG + +GT+DGAN E+ E+ GE+   +   R  ++A
Sbjct:  659 PATDLSEQISTAGTEASGTGNMKFMQNGALTIGTMDGANVEMAEEAGEENLFIFGMRVEDVA 720

HSP 2 Score: 38.1206 bits (87), Expect = 1.907e-13
Identity = 25/64 (39.06%), Postives = 32/64 (50.00%), Query Frame = 1

Query:   64 LKSIFELERQLMTI*RIVDTYKEMLEMSAVKRNAK--FVPRVCIFGGKAFASYVQANTIGKFIT 249
            +K I E +RQL+    +V  Y        +K++ K  FVPR  I GGKA   Y  A  I K IT
Sbjct:  568 VKRIHEYKRQLLNCLHVVTMYNR------IKKDPKKLFVPRTVIIGGKAAPGYYMAKLIIKLIT 625

[Close]

BLAST of EY660263 vs. ExPASy Swiss-Prot
Match: PYGL_BOVIN (Glycogen phosphorylase, liver form OS=Bos taurus GN=PYGL PE=2 SV=1)

HSP 1 Score: 57.3806 bits (137), Expect = 5.425e-13
Identity = 27/62 (43.55%), Postives = 41/62 (66.13%), Query Frame = 3

Query:  351 PASELSQHISTAWMEARATTDVKFAKNGCMVVGTLDGANGEIREQVGEDIFLLLCDRAYEIA 536
            PA++LS+ ISTA  EA  T ++KF  NG + +GT+DGAN E+ E+ GE+   +   R  ++A
Sbjct:  659 PATDLSEQISTAGTEASGTGNMKFMLNGALTIGTMDGANVEMAEEAGEENLFIFGMRIEDVA 720

HSP 2 Score: 38.1206 bits (87), Expect = 5.425e-13
Identity = 25/64 (39.06%), Postives = 32/64 (50.00%), Query Frame = 1

Query:   64 LKSIFELERQLMTI*RIVDTYKEMLEMSAVKRNAK--FVPRVCIFGGKAFASYVQANTIGKFIT 249
            +K I E +RQL+    +V  Y        +K++ K  FVPR  I GGKA   Y  A  I K IT
Sbjct:  568 VKRIHEYKRQLLNCLHVVTMYNR------IKKDPKKLFVPRTVIIGGKAAPGYYMAKLIIKLIT 625

[Close]

BLAST of EY660263 vs. ExPASy Swiss-Prot
Match: PYGM_RAT (Glycogen phosphorylase, muscle form OS=Rattus norvegicus GN=Pygm PE=2 SV=5)

HSP 1 Score: 60.077 bits (144), Expect = 9.152e-13
Identity = 28/61 (45.90%), Postives = 41/61 (67.21%), Query Frame = 3

Query:  351 PASELSQHISTAWMEARATTDVKFAKNGCMVVGTLDGANGEIREQVGEDIFLLLCDRAYEI 533
            PA++LS+ ISTA  EA  T ++KF  NG + +GT+DGAN E+ E+ GED F +   R  ++
Sbjct:  659 PAADLSEQISTAGTEASGTGNMKFMLNGALTIGTMDGANVEMAEEAGEDNFFIFGMRVEDV 719

HSP 2 Score: 34.6538 bits (78), Expect = 9.152e-13
Identity = 24/68 (35.29%), Postives = 31/68 (45.59%), Query Frame = 1

Query:   64 LKSIFELERQLMTI*RIVDTYKEMLEMSAVKRNAKFVPRVCIFGGKAFASYVQANTIGKFITDGGATV 267
            +K I E +RQL+    I+  Y  +      + N   VPR  + GGKA   Y  A  I K IT  G  V
Sbjct:  568 VKRIHEYKRQLLNCLHIITLYNRIKR----EPNRFMVPRTIMIGGKAAPGYHMAKMIIKLITAIGDVV 631

The following BLAST results are available for this feature:

BLAST of EY660263 vs. ExPASy Swiss-Prot

BLAST of EY660263 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 33


Match Name	E-value	Identity	Description
PHSG_YEAST	3.512e-16	56.60	Glycogen phosphorylase OS=Saccharomyces cerevisiae...	[more]
PHS1_DICDI	3.520e-16	47.54	Glycogen phosphorylase 1 OS=Dictyostelium discoide...	[more]
PHSG_PASMU	5.690e-16	53.06	Glycogen phosphorylase OS=Pasteurella multocida GN...	[more]
PHSG_SYNY3	2.140e-15	56.60	Glycogen phosphorylase OS=Synechocystis sp. (strai...	[more]
PHSG_CHLTR	6.364e-15	48.44	Glycogen phosphorylase OS=Chlamydia trachomatis GN...	[more]
PHSM_ECOLI	7.777e-15	57.14	Maltodextrin phosphorylase OS=Escherichia coli (st...	[more]
PHSG_CHLMU	8.268e-15	53.06	Glycogen phosphorylase OS=Chlamydia muridarum GN=g...	[more]
PYGL_SHEEP	1.907e-13	43.55	Glycogen phosphorylase, liver form OS=Ovis aries G...	[more]
PYGL_BOVIN	5.425e-13	43.55	Glycogen phosphorylase, liver form OS=Bos taurus G...	[more]
PYGM_RAT	9.152e-13	45.90	Glycogen phosphorylase, muscle form OS=Rattus norv...	[more]

Pages

Properties


Property Name	Value
Genbank description	CS00-C1-101-020-C09-CT.F Sweet orange leaf, infected with Xylella fastidiosa (stage 1 of 2) Citrus sinensis cDNA, mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>EY660263 ID=EY660263; Name=EY660263; organism=Citrus sinensis; type=EST; length=918bp
ACTGGTCGCCTGCGGTACTGGTCCGGAATTTTTTCAAAGACGGCGTGTCC
GATGACATCCTGTCTGAAGAGCATCTTTGAACTCGAGCGACAATTGATGA
CTATCTAGAGAATTGTTGACACATACAAAGAGATGTTAGAAATGAGTGCA
GTAAAAAGGAATGCCAAATTTGTACCAAGAGTTTGTATATTTGGAGGAAA
AGCCTTTGCTAGCTATGTGCAAGCCAATACAATTGGGAAATTTATTACGG
ATGGTGGGGCCACGGTCTGTCTTGGTCCTGAAGATAGGTGACTTATTGAA
GGGAATTTTTGTCCCTGATTACAATGTACGTGAGGCTGAATTGCTTATAG
CCTGCAAGTGAGCTCTCTCAACACATCAGTACTGCCTGGATGGAGGCTAG
AGCTACCACTGACGTGAAATTTGCAAAGAATGGTTGCATGGTGGTTGGAA
CCTTGGATGGTGCCAATGGTGAAATACGGGAACAGGTTGGAGAGGATATC
TTTTTACTATTATGTGACAGAGCCTACGAGATTGCAGGATAGTTATAACA
AAGATCTGAGGGGAAATTTGTTCCCTCACACCGATTTGAAGAATTGCACA
AATTTGCCAGATCAGGTGGTTTTGGGTGTTACTGCTATGATGAATCTATG
GGATCACTGGAACGAAATGAATGCTTTGGCCGTGCACATTATTGCCAACG
AGTGCACAGATTTTCCTAGCTACTTAGAATGCCAAGATAAAGTTTTTGAA
GCGTGTTGCCATCTAAATAGATGGACGAGAATGACGATCATGAACACATC
GTGCTCCTTGAAGATGAACACTAACAGAACGAGTCGGGATTGTGCCCGAA
CATATGGAACTTAATACCCGTGCAATTGCCGAGAACGTGTAATGGGTGTT
CCCAGTAACTGAGNTGGG