EY660088

Overview
NameEY660088
Unique NameEY660088
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length891
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of EY660088 vs. ExPASy Swiss-Prot
Match: GLYA_BACFR (Serine hydroxymethyltransferase OS=Bacteroides fragilis GN=glyA PE=3 SV=1)

HSP 1 Score: 223.016 bits (567), Expect = 1.782e-57
Identity = 112/228 (49.12%), Postives = 155/228 (67.98%), Query Frame = 3 
Query:   12 QWKGLELIPSENFTSVSVMQAVGSVMTNKYSEGYPGARYYGGNEYIDMAESLCQKRALEAFRLDPEKWGVNVQSLSGSPSNFQVYTALLKPHDRIMALDLPHGGHLSHGYQTDTKKISAVSIFFETMPYRLNESTGYIDYDQLEKSATLFRPKLIVAGASAYARLYDYERIRKVCNKQKAIMLADMAHISGLVAAGVIPSPFEYADVVTTTTHKSLRGPRGAMIFFRK 695
            Q KG+ELI SENF S  VM+A+GS +TNKY+EGYPG RYYGG E +D +E +   R  E F  +   W  NVQ  SG+ +N  V+ A+L P D+ M L+L HGGHLSHG   +T       I +    Y L + TG +DYDQ+E+ A   +PK+I+ G SAY+R +DY+R+R++ +K  AI++ DMAH +GL+AAG++ +P +YA +VT+TTHK+LRGPRG +I   K
Sbjct:   18 QLKGIELIASENFVSDQVMEAMGSCLTNKYAEGYPGKRYYGGCEVVDQSEQIAIDRLKEIFGAE---WA-NVQPHSGAQANAAVFLAVLNPGDKFMGLNLAHGGHLSHGSLVNTS-----GIIYTPCEYNLKQETGRVDYDQMEEVALREKPKMIIGGGSAYSREWDYKRMREIADKVGAILMIDMAHPAGLIAAGLLDNPVKYAHIVTSTTHKTLRGPRGGVIMMGK 236
[Close]
BLAST of EY660088 vs. ExPASy Swiss-Prot
Match: GLYA_BACFN (Serine hydroxymethyltransferase OS=Bacteroides fragilis (strain ATCC 25285 / NCTC 9343) GN=glyA PE=3 SV=1)

HSP 1 Score: 223.016 bits (567), Expect = 1.782e-57
Identity = 112/228 (49.12%), Postives = 155/228 (67.98%), Query Frame = 3 
Query:   12 QWKGLELIPSENFTSVSVMQAVGSVMTNKYSEGYPGARYYGGNEYIDMAESLCQKRALEAFRLDPEKWGVNVQSLSGSPSNFQVYTALLKPHDRIMALDLPHGGHLSHGYQTDTKKISAVSIFFETMPYRLNESTGYIDYDQLEKSATLFRPKLIVAGASAYARLYDYERIRKVCNKQKAIMLADMAHISGLVAAGVIPSPFEYADVVTTTTHKSLRGPRGAMIFFRK 695
            Q KG+ELI SENF S  VM+A+GS +TNKY+EGYPG RYYGG E +D +E +   R  E F  +   W  NVQ  SG+ +N  V+ A+L P D+ M L+L HGGHLSHG   +T       I +    Y L + TG +DYDQ+E+ A   +PK+I+ G SAY+R +DY+R+R++ +K  AI++ DMAH +GL+AAG++ +P +YA +VT+TTHK+LRGPRG +I   K
Sbjct:   18 QLKGIELIASENFVSDQVMEAMGSCLTNKYAEGYPGKRYYGGCEVVDQSEQIAIDRLKEIFGAE---WA-NVQPHSGAQANAAVFLAVLNPGDKFMGLNLAHGGHLSHGSLVNTS-----GIIYTPCEYNLKQETGRVDYDQMEEVALREKPKMIIGGGSAYSREWDYKRMREIADKVGAILMIDMAHPAGLIAAGLLDNPVKYAHIVTSTTHKTLRGPRGGVIMMGK 236
[Close]
BLAST of EY660088 vs. ExPASy Swiss-Prot
Match: GLYA_ACIF5 (Serine hydroxymethyltransferase OS=Acidithiobacillus ferrooxidans (strain ATCC 53993) GN=glyA PE=3 SV=1)

HSP 1 Score: 223.016 bits (567), Expect = 1.782e-57
Identity = 118/230 (51.30%), Postives = 152/230 (66.09%), Query Frame = 3 
Query:    6 RPQWKGLELIPSENFTSVSVMQAVGSVMTNKYSEGYPGARYYGGNEYIDMAESLCQKRALEAFRLDPEKWGVNVQSLSGSPSNFQVYTALLKPHDRIMALDLPHGGHLSHGYQTDTKKISAVSIFFETMPYRLNESTGYIDYDQLEKSATLFRPKLIVAGASAYARLYDYERIRKVCNKQKAIMLADMAHISGLVAAGVIPSPFEYADVVTTTTHKSLRGPRGAMIFFRK 695
            R Q   +ELI SEN+ S  VM A GSV+TNKY+EGYPG RYYGG EY+D+AE L   RALE F  +      NVQ+ SGS +N  VY ++L+P D+IM + L HGGHL+HG      K++     F+   Y +    G IDYD + + A   RPK+IVAGASAY+R+ D+ RI ++     A +L DMAHI+GLVA G+ PSP  +AD VTTTTHK+LRGPRG +I  R+
Sbjct:   24 RRQEDHVELIASENYASPMVMAAQGSVLTNKYAEGYPGKRYYGGCEYVDIAEQLAMDRALELFGAEH----ANVQAHSGSQANQAVYLSVLQPGDKIMGMSLAHGGHLTHG-----AKVNVSGKLFQVAAYGVRAEDGRIDYDAMAEQAERERPKMIVAGASAYSRVIDFARIGEIARSIGAYLLVDMAHIAGLVATGLHPSPVPHADFVTTTTHKTLRGPRGGLILCRE 244
[Close]
BLAST of EY660088 vs. ExPASy Swiss-Prot
Match: GLYA_ACIF2 (Serine hydroxymethyltransferase OS=Acidithiobacillus ferrooxidans (strain ATCC 23270 / DSM 14882 / NCIB 8455) GN=glyA PE=3 SV=1)

HSP 1 Score: 223.016 bits (567), Expect = 1.782e-57
Identity = 118/230 (51.30%), Postives = 152/230 (66.09%), Query Frame = 3 
Query:    6 RPQWKGLELIPSENFTSVSVMQAVGSVMTNKYSEGYPGARYYGGNEYIDMAESLCQKRALEAFRLDPEKWGVNVQSLSGSPSNFQVYTALLKPHDRIMALDLPHGGHLSHGYQTDTKKISAVSIFFETMPYRLNESTGYIDYDQLEKSATLFRPKLIVAGASAYARLYDYERIRKVCNKQKAIMLADMAHISGLVAAGVIPSPFEYADVVTTTTHKSLRGPRGAMIFFRK 695
            R Q   +ELI SEN+ S  VM A GSV+TNKY+EGYPG RYYGG EY+D+AE L   RALE F  +      NVQ+ SGS +N  VY ++L+P D+IM + L HGGHL+HG      K++     F+   Y +    G IDYD + + A   RPK+IVAGASAY+R+ D+ RI ++     A +L DMAHI+GLVA G+ PSP  +AD VTTTTHK+LRGPRG +I  R+
Sbjct:   24 RRQEDHVELIASENYASPMVMAAQGSVLTNKYAEGYPGKRYYGGCEYVDIAEQLAMDRALELFGAEH----ANVQAHSGSQANQAVYLSVLQPGDKIMGMSLAHGGHLTHG-----AKVNVSGKLFQVAAYGVRAEDGRIDYDAMAEQAERERPKMIVAGASAYSRVIDFARIGEIARSIGAYLLVDMAHIAGLVATGLHPSPVPHADFVTTTTHKTLRGPRGGLILCRE 244
[Close]
BLAST of EY660088 vs. ExPASy Swiss-Prot
Match: GLYA_THENN (Serine hydroxymethyltransferase OS=Thermotoga neapolitana (strain ATCC 49049 / DSM 4359 / NS-E) GN=glyA PE=3 SV=1)

HSP 1 Score: 222.631 bits (566), Expect = 2.327e-57
Identity = 116/226 (51.33%), Postives = 154/226 (68.14%), Query Frame = 3 
Query:    6 RPQWKGLELIPSENFTSVSVMQAVGSVMTNKYSEGYPGARYYGGNEYIDMAESLCQKRALEAFRLDPEKWGVNVQSLSGSPSNFQVYTALLKPHDRIMALDLPHGGHLSHGYQTDTKKISAVSIFFETMPYRLNESTGYIDYDQLEKSATLFRPKLIVAGASAYARLYDYERIRKVCNKQKAIMLADMAHISGLVAAGVIPSPFEYADVVTTTTHKSLRGPRGAMI 683
            R Q  GLELI SENF S++V++ +GSV+TNKY+EGYPG RYYGG E++D AE L  +RA + F  +      NVQ  SGS +N  VY AL +P D IM + L HGGHL+HG       ++     F+ + Y +N  T  IDYD++ K A   RPK+IVAG SAYAR  D++R R++ ++  A ++ DMAH +GLVAAG+ P+P EYA VVT+TTHK+LRGPRG +I
Sbjct:   21 RRQEYGLELIASENFASLAVIETMGSVLTNKYAEGYPGRRYYGGCEWVDRAEELAIERAKKLFGAE----FANVQPHSGSQANMAVYLALAQPGDTIMGMSLSHGGHLTHG-----APVNFSGRIFKVVHYGVNLETETIDYDEVRKLALEHRPKIIVAGGSAYARTIDFKRFREIADEVGAYLMVDMAHFAGLVAAGIHPNPVEYAHVVTSTTHKTLRGPRGGLI 237
[Close]
BLAST of EY660088 vs. ExPASy Swiss-Prot
Match: GLYA_PSEU5 (Serine hydroxymethyltransferase OS=Pseudomonas stutzeri (strain A1501) GN=glyA PE=3 SV=1)

HSP 1 Score: 222.631 bits (566), Expect = 2.327e-57
Identity = 122/255 (47.84%), Postives = 161/255 (63.14%), Query Frame = 3 
Query:   24 LELIPSENFTSVSVMQAVGSVMTNKYSEGYPGARYYGGNEYIDMAESLCQKRALEAFRLDPEKWGVNVQSLSGSPSNFQVYTALLKPHDRIMALDLPHGGHLSHGYQTDTKKISAVSIFFETMPYRLNESTGYIDYDQLEKSATLFRPKLIVAGASAYARLYDYERIRKVCNKQKAIMLADMAHISGLVAAGVIPSPFEYADVVTTTTHKSLRGPRGAMIFFRKGVEEITXQGXECFMIMKKK*SSCLSWTPGGP 788
            +ELI SEN+TS +VM+A GSV+TNKY+EGYPG RYYGG EY+D+ E L   RA E F  D      NVQ  +GS +N  VY ALL   D I+ + L HGGHL+HG       +S+    +  + Y +N+  G IDYD++E+ A   +PK+IVAG SAY++  D+ R R++ +K  A +  DMAH++GLVAAGV P+P  +ADVVTTTTHK+LRGPRG +I  +K  E           I KK  S+      GGP
Sbjct:   30 IELIASENYTSPAVMEAQGSVLTNKYAEGYPGKRYYGGCEYVDVVEQLAIDRAKELFGADY----ANVQPHAGSQANAAVYLALLSAGDTILGMSLAHGGHLTHG-----ASVSSSGKLYNAVQYGINDQ-GLIDYDEVERLAVEHKPKMIVAGFSAYSQKLDFARFREIADKVGAYLFVDMAHVAGLVAAGVYPNPVPFADVVTTTTHKTLRGPRGGLILAKKNEE-----------IEKKLNSAVFPGAQGGP 263
[Close]
BLAST of EY660088 vs. ExPASy Swiss-Prot
Match: GLYA_CLOK5 (Serine hydroxymethyltransferase OS=Clostridium kluyveri (strain ATCC 8527 / DSM 555 / NCIMB 10680) GN=glyA PE=3 SV=1)

HSP 1 Score: 222.631 bits (566), Expect = 2.327e-57
Identity = 114/228 (50.00%), Postives = 153/228 (67.11%), Query Frame = 3 
Query:   12 QWKGLELIPSENFTSVSVMQAVGSVMTNKYSEGYPGARYYGGNEYIDMAESLCQKRALEAFRLDPEKWGVNVQSLSGSPSNFQVYTALLKPHDRIMALDLPHGGHLSHGYQTDTKKISAVSIFFETMPYRLNESTGYIDYDQLEKSATLFRPKLIVAGASAYARLYDYERIRKVCNKQKAIMLADMAHISGLVAAGVIPSPFEYADVVTTTTHKSLRGPRGAMIFFRK 695
            Q  G+ELI SENFTS SVM+A+GS +TNKY+EGYPG RYYGG   +D AE L + R  + F  +     VNVQ  SGS +N  VY ++LKP D ++ + L HGGHL+HG      K+S     +  + Y LN  T  IDYD++ + A   +PK+IV+GASAY R  D+++IR++C++  A M+ DMAHI+G++AAG   SP  YAD VTTTTHK+LRGPRG  I  ++
Sbjct:   24 QKNGIELIASENFTSKSVMEAMGSFLTNKYAEGYPGKRYYGGCYIVDKAEDLARDRMKKLFNAEH----VNVQPHSGSQANMAVYMSVLKPGDTVLGMSLNHGGHLTHG-----SKVSFSGKLYNFVSYGLNSDTEIIDYDEMRELALKHKPKMIVSGASAYPRKIDFKKIREICDEVGAYMMVDMAHIAGIIAAGRHESPVPYADFVTTTTHKTLRGPRGGAIICKE 242
[Close]
BLAST of EY660088 vs. ExPASy Swiss-Prot
Match: GLYA_CLOK1 (Serine hydroxymethyltransferase OS=Clostridium kluyveri (strain NBRC 12016) GN=glyA PE=3 SV=1)

HSP 1 Score: 222.631 bits (566), Expect = 2.327e-57
Identity = 114/228 (50.00%), Postives = 153/228 (67.11%), Query Frame = 3 
Query:   12 QWKGLELIPSENFTSVSVMQAVGSVMTNKYSEGYPGARYYGGNEYIDMAESLCQKRALEAFRLDPEKWGVNVQSLSGSPSNFQVYTALLKPHDRIMALDLPHGGHLSHGYQTDTKKISAVSIFFETMPYRLNESTGYIDYDQLEKSATLFRPKLIVAGASAYARLYDYERIRKVCNKQKAIMLADMAHISGLVAAGVIPSPFEYADVVTTTTHKSLRGPRGAMIFFRK 695
            Q  G+ELI SENFTS SVM+A+GS +TNKY+EGYPG RYYGG   +D AE L + R  + F  +     VNVQ  SGS +N  VY ++LKP D ++ + L HGGHL+HG      K+S     +  + Y LN  T  IDYD++ + A   +PK+IV+GASAY R  D+++IR++C++  A M+ DMAHI+G++AAG   SP  YAD VTTTTHK+LRGPRG  I  ++
Sbjct:   24 QKNGIELIASENFTSKSVMEAMGSFLTNKYAEGYPGKRYYGGCYIVDKAEDLARDRMKKLFNAEH----VNVQPHSGSQANMAVYMSVLKPGDTVLGMSLNHGGHLTHG-----SKVSFSGKLYNFVSYGLNSDTEIIDYDEMRELALKHKPKMIVSGASAYPRKIDFKKIREICDEVGAYMMVDMAHIAGIIAAGRHESPVPYADFVTTTTHKTLRGPRGGAIICKE 242
[Close]
BLAST of EY660088 vs. ExPASy Swiss-Prot
Match: GLYA2_COLP3 (Serine hydroxymethyltransferase 2 OS=Colwellia psychrerythraea (strain 34H / ATCC BAA-681) GN=glyA2 PE=3 SV=1)

HSP 1 Score: 222.246 bits (565), Expect = 3.039e-57
Identity = 114/227 (50.22%), Postives = 154/227 (67.84%), Query Frame = 3 
Query:   24 LELIPSENFTSVSVMQAVGSVMTNKYSEGYPGARYYGGNEYIDMAESLCQKRALEAFRLDPEKWGVNVQSLSGSPSNFQVYTALLKPHDRIMALDLPHGGHLSHGYQTDTKKISAVSIFFETMPYRLNESTGYIDYDQLEKSATLFRPKLIVAGASAYARLYDYERIRKVCNKQKAIMLADMAHISGLVAAGVIPSPFEYADVVTTTTHKSLRGPRGAMIFFRKGVE 704
            +ELI SEN+TS  VMQA GS +TNKY+EGYPG RYYGG E++D+ E L   RA E F  D      NVQ  SGS +N  V+ ALLKP + ++ + L HGGHL+HG      K+S     +  + Y LNE+TG IDY+++E+ A   +PK+I+AG SAY+R+ D++R R + +   A +  DMAH++GLVAAG+ P+P   ADVVTTTTHK+LRGPRG +I  ++  E
Sbjct:   30 VELIASENYTSARVMQAQGSQLTNKYAEGYPGKRYYGGCEHVDVIEQLAIDRAKELFGADY----ANVQPHSGSQANAAVFMALLKPGETVLGMSLAHGGHLTHG-----SKVSFSGKIYNAVQYGLNEATGEIDYEEVERLAKEHQPKMIIAGFSAYSRVVDWQRFRDIADSIGAWLFVDMAHVAGLVAAGLYPNPVPIADVVTTTTHKTLRGPRGGLILAKQNDE 247
[Close]
BLAST of EY660088 vs. ExPASy Swiss-Prot
Match: GLYA_SHISS (Serine hydroxymethyltransferase OS=Shigella sonnei (strain Ss046) GN=glyA PE=3 SV=1)

HSP 1 Score: 221.861 bits (564), Expect = 3.969e-57
Identity = 115/233 (49.36%), Postives = 157/233 (67.38%), Query Frame = 3 
Query:   12 QWKGLELIPSENFTSVSVMQAVGSVMTNKYSEGYPGARYYGGNEYIDMAESLCQKRALEAFRLDPEKWGVNVQSLSGSPSNFQVYTALLKPHDRIMALDLPHGGHLSHGYQTDTKKISAVSIFFETMPYRLNESTGYIDYDQLEKSATLFRPKLIVAGASAYARLYDYERIRKVCNKQKAIMLADMAHISGLVAAGVIPSPFEYADVVTTTTHKSLRGPRGAMIFFRKGVEEI 710
            Q + +ELI SEN+TS  VMQA GS +TNKY+EGYPG RYYGG EY+D+ E L   RA E F  D      NVQ  SGS +NF VYTALL+P D ++ ++L HGGHL+HG       ++     +  +PY + ++TG+IDY  LEK A   +PK+I+ G SAY+ + D+ ++R++ +   A +  DMAH++GLVAAGV P+P  +A VVTTTTHK+L GPRG +I  + G EE+
Sbjct:   26 QEEHIELIASENYTSPRVMQAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRAKELFGADY----ANVQPHSGSQANFAVYTALLEPGDTVLGMNLAHGGHLTHG-----SPVNFSGKLYNIVPYGI-DATGHIDYADLEKQAKEHKPKMIIGGFSAYSGVVDWAKMREIADSIGAYLFVDMAHVAGLVAAGVYPNPVPHAHVVTTTTHKTLAGPRGGLILAKGGSEEL 248
The following BLAST results are available for this feature:
BLAST of EY660088 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 500
Match NameE-valueIdentityDescription
GLYA_BACFR1.782e-5749.12Serine hydroxymethyltransferase OS=Bacteroides fra... [more]
GLYA_BACFN1.782e-5749.12Serine hydroxymethyltransferase OS=Bacteroides fra... [more]
GLYA_ACIF51.782e-5751.30Serine hydroxymethyltransferase OS=Acidithiobacill... [more]
GLYA_ACIF21.782e-5751.30Serine hydroxymethyltransferase OS=Acidithiobacill... [more]
GLYA_THENN2.327e-5751.33Serine hydroxymethyltransferase OS=Thermotoga neap... [more]
GLYA_PSEU52.327e-5747.84Serine hydroxymethyltransferase OS=Pseudomonas stu... [more]
GLYA_CLOK52.327e-5750.00Serine hydroxymethyltransferase OS=Clostridium klu... [more]
GLYA_CLOK12.327e-5750.00Serine hydroxymethyltransferase OS=Clostridium klu... [more]
GLYA2_COLP33.039e-5750.22Serine hydroxymethyltransferase 2 OS=Colwellia psy... [more]
GLYA_SHISS3.969e-5749.36Serine hydroxymethyltransferase OS=Shigella sonnei... [more]

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Properties
Property NameValue
Genbank descriptionCS00-C1-101-018-D05-CT.F Sweet orange leaf, infected with Xylella fastidiosa (stage 1 of 2) Citrus sinensis cDNA, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>EY660088 ID=EY660088; Name=EY660088; organism=Citrus sinensis; type=EST; length=891bp
CCACGCGTCCGCAATGGAAGGGACTTGAACTCATTCCTTCAGAGAACTTT
ACTTCAGTATCCGTGATGCAAGCTGTGGGGTCTGTCATGACCAACAAGTA
CAGTGAAGGATATCCAGGTGCAAGATATTATGGAGGAAACGAGTATATTG
ACATGGCGGAATCCTTATGTCAGAAGCGAGCTCTGGAAGCATTTCGGTTG
GATCCTGAAAAATGGGGAGTGAATGTGCAGTCTCTGTCTGGATCTCCATC
TAATTTTCAAGTCTACACTGCATTGTTAAAACCTCATGATAGGATCATGG
CACTTGATCTTCCTCATGGTGGGCATCTCTCACACGGCTATCAGACAGAT
ACCAAAAAGATATCTGCAGTGTCCATATTTTTTGAGACAATGCCATACAG
ATTGAATGAGAGCACTGGCTATATAGACTATGACCAGTTGGAGAAAAGTG
CCACCCTCTTCAGGCCAAAATTGATTGTTGCTGGTGCTAGTGCTTATGCA
CGATTGTATGATTATGAGCGTATTCGCAAGGTTTGCAACAAACAAAAAGC
TATAATGTTGGCAGATATGGCACACATTAGTGGTTTGGTTGCTGCTGGTG
TCATCCCTTCACCTTTTGAGTATGCAGATGTTGTGACCACCACAACTCAC
AAATCACTTCGTGGGCCACGTGGAGCCATGATTTTCTTTAGGAAGGGAGT
NGAAGAGATTACAANNCAGGGAANNGAGTGTTTTATGATTATGAAGAAAA
AATAATCAAGCTGTCTTTCCTGGACTCCAGGTGGCCCACACACCACACAA
TTACTGGNTTAGCANGTGCCTTGAACAGGCACTACTCCGAGTATAAAGCT
ATCAGGAGCAAGTCTGAGCAATGCTCAAAATTGCACGATCT
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