EY659990

Overview
NameEY659990
Unique NameEY659990
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length932
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of EY659990 vs. ExPASy Swiss-Prot
Match: GLYA_PELLD (Serine hydroxymethyltransferase OS=Pelodictyon luteolum (strain DSM 273) GN=glyA PE=3 SV=1)

HSP 1 Score: 146.362 bits (368), Expect = 1.035e-36
Identity = 81/168 (48.21%), Postives = 108/168 (64.29%), Query Frame = 2 
Query:  227 LEVVDPEIADIIEHEKARQWKGLELIPSENFTSVSVMQAVGSVMTNNHSEGYPGARYYGGNEYIDMAESLCQKRALEAFRLDPEKWGVNVQSLSGSPSNFQVYTALLKPHDRIMALDLPHGGHLSHGYQTDTKKISAVSIFFETMPYRLNESTGYIDYDQLEKSATLF 730
            L++ D E+ D I  E  RQ + LELI SENFTS +VMQA GSVMTN ++EGYPG RYYGG E++D+AE L ++RA + F  +     VNVQ  SGS +N  V  ++LKP DRIM LDL HGGHL+HG       ++     F+   Y ++  TG ID +++E+ A  F
Sbjct:    6 LKMQDREVFDAIAGETVRQMETLELIASENFTSRAVMQACGSVMTNKYAEGYPGKRYYGGCEFVDIAEDLARERARKLFGCE----YVNVQPHSGSSANMAVLFSVLKPGDRIMGLDLSHGGHLTHG-----SSVNFSGQMFDARSYGVDRETGIIDMNKVEEMALDF 164

HSP 2 Score: 28.8758 bits (63), Expect = 1.035e-36
Identity = 10/25 (40.00%), Postives = 20/25 (80.00%), Query Frame = 1 
Query:  736 KLIVAGASAYARLYDYERIRKVCNK 810
            +LI+ GASAY++ +D++  R++ +K
Sbjct:  167 RLIICGASAYSQGFDFKAFREIADK 191
[Close]
BLAST of EY659990 vs. ExPASy Swiss-Prot
Match: GLYA_BACFR (Serine hydroxymethyltransferase OS=Bacteroides fragilis GN=glyA PE=3 SV=1)

HSP 1 Score: 141.739 bits (356), Expect = 1.036e-36
Identity = 77/161 (47.83%), Postives = 99/161 (61.49%), Query Frame = 2 
Query:  239 DPEIADIIEHEKARQWKGLELIPSENFTSVSVMQAVGSVMTNNHSEGYPGARYYGGNEYIDMAESLCQKRALEAFRLDPEKWGVNVQSLSGSPSNFQVYTALLKPHDRIMALDLPHGGHLSHGYQTDTKKISAVSIFFETMPYRLNESTGYIDYDQLEKSA 721
            D  I DIIE E  RQ KG+ELI SENF S  VM+A+GS +TN ++EGYPG RYYGG E +D +E +   R  E F  +   W  NVQ  SG+ +N  V+ A+L P D+ M L+L HGGHLSHG   +T       I +    Y L + TG +DYDQ+E+ A
Sbjct:    4 DDLIFDIIEKEHQRQLKGIELIASENFVSDQVMEAMGSCLTNKYAEGYPGKRYYGGCEVVDQSEQIAIDRLKEIFGAE---W-ANVQPHSGAQANAAVFLAVLNPGDKFMGLNLAHGGHLSHGSLVNTS-----GIIYTPCEYNLKQETGRVDYDQMEEVA 155

HSP 2 Score: 33.4982 bits (75), Expect = 1.036e-36
Identity = 12/25 (48.00%), Postives = 21/25 (84.00%), Query Frame = 1 
Query:  736 KLIVAGASAYARLYDYERIRKVCNK 810
            K+I+ G SAY+R +DY+R+R++ +K
Sbjct:  161 KMIIGGGSAYSREWDYKRMREIADK 185
[Close]
BLAST of EY659990 vs. ExPASy Swiss-Prot
Match: GLYA_BACFN (Serine hydroxymethyltransferase OS=Bacteroides fragilis (strain ATCC 25285 / NCTC 9343) GN=glyA PE=3 SV=1)

HSP 1 Score: 141.739 bits (356), Expect = 1.036e-36
Identity = 77/161 (47.83%), Postives = 99/161 (61.49%), Query Frame = 2 
Query:  239 DPEIADIIEHEKARQWKGLELIPSENFTSVSVMQAVGSVMTNNHSEGYPGARYYGGNEYIDMAESLCQKRALEAFRLDPEKWGVNVQSLSGSPSNFQVYTALLKPHDRIMALDLPHGGHLSHGYQTDTKKISAVSIFFETMPYRLNESTGYIDYDQLEKSA 721
            D  I DIIE E  RQ KG+ELI SENF S  VM+A+GS +TN ++EGYPG RYYGG E +D +E +   R  E F  +   W  NVQ  SG+ +N  V+ A+L P D+ M L+L HGGHLSHG   +T       I +    Y L + TG +DYDQ+E+ A
Sbjct:    4 DDLIFDIIEKEHQRQLKGIELIASENFVSDQVMEAMGSCLTNKYAEGYPGKRYYGGCEVVDQSEQIAIDRLKEIFGAE---W-ANVQPHSGAQANAAVFLAVLNPGDKFMGLNLAHGGHLSHGSLVNTS-----GIIYTPCEYNLKQETGRVDYDQMEEVA 155

HSP 2 Score: 33.4982 bits (75), Expect = 1.036e-36
Identity = 12/25 (48.00%), Postives = 21/25 (84.00%), Query Frame = 1 
Query:  736 KLIVAGASAYARLYDYERIRKVCNK 810
            K+I+ G SAY+R +DY+R+R++ +K
Sbjct:  161 KMIIGGGSAYSREWDYKRMREIADK 185
[Close]
BLAST of EY659990 vs. ExPASy Swiss-Prot
Match: GLYA_THEPX (Serine hydroxymethyltransferase OS=Thermoanaerobacter sp. (strain X514) GN=glyA PE=3 SV=1)

HSP 1 Score: 154.066 bits (388), Expect = 1.085e-36
Identity = 92/232 (39.66%), Postives = 124/232 (53.45%), Query Frame = 2 
Query:  239 DPEIADIIEHEKARQWKGLELIPSENFTSVSVMQAVGSVMTNNHSEGYPGARYYGGNEYIDMAESLCQKRALEAFRLDPEKWGVNVQSLSGSPSNFQVYTALLKPHDRIMALDLPHGGHLSHGYQTDTKKISAVSIFFETMPYRLNESTGYIDYDQLEKSATLFXXNXXXXXXXXMHDCTIMSVFARFATN-KSYNVGRMAHLXWVGCCWCHPFTFENADGVTTTLTNTSWG 931
            DPEIAD IE E  RQ   +ELI SENF S +VM+A+GS +TN ++EGYP  RYYGG EY+D+AE L ++R  + F  +      NVQ  SG+ +N   Y AL+KP D ++ +DL HGGHL+HG      K++     +  + Y + E TGYIDYD++E+ A                       F   A    +Y +  MAH+  +     HP     AD VTTT   T  G
Sbjct:   10 DPEIADAIEKELIRQRNKIELIASENFVSRAVMEAMGSPLTNKYAEGYPNKRYYGGCEYVDIAEELARERLKKLFGAE----HANVQPHSGAQANMAAYFALIKPGDTVLGMDLAHGGHLTHG-----SKVNFSGQIYNFVSYGVREDTGYIDYDEVERVAKKHKPKLIVAGASAYPRIIDFKRFREIADKVGAYLMVDMAHIAGLVAAGLHPNPVPYADVVTTTTHKTLRG 232
[Close]
BLAST of EY659990 vs. ExPASy Swiss-Prot
Match: GLYA_THEP3 (Serine hydroxymethyltransferase OS=Thermoanaerobacter pseudethanolicus (strain ATCC 33223 / 39E) GN=glyA PE=3 SV=1)

HSP 1 Score: 154.066 bits (388), Expect = 1.085e-36
Identity = 92/232 (39.66%), Postives = 124/232 (53.45%), Query Frame = 2 
Query:  239 DPEIADIIEHEKARQWKGLELIPSENFTSVSVMQAVGSVMTNNHSEGYPGARYYGGNEYIDMAESLCQKRALEAFRLDPEKWGVNVQSLSGSPSNFQVYTALLKPHDRIMALDLPHGGHLSHGYQTDTKKISAVSIFFETMPYRLNESTGYIDYDQLEKSATLFXXNXXXXXXXXMHDCTIMSVFARFATN-KSYNVGRMAHLXWVGCCWCHPFTFENADGVTTTLTNTSWG 931
            DPEIAD IE E  RQ   +ELI SENF S +VM+A+GS +TN ++EGYP  RYYGG EY+D+AE L ++R  + F  +      NVQ  SG+ +N   Y AL+KP D ++ +DL HGGHL+HG      K++     +  + Y + E TGYIDYD++E+ A                       F   A    +Y +  MAH+  +     HP     AD VTTT   T  G
Sbjct:   10 DPEIADAIEKELIRQRNKIELIASENFVSRAVMEAMGSPLTNKYAEGYPNKRYYGGCEYVDIAEELARERLKKLFGAE----HANVQPHSGAQANMAAYFALIKPGDTVLGMDLAHGGHLTHG-----SKVNFSGQIYNFVSYGVREDTGYIDYDEVERVAKKHKPKLIVAGASAYPRIIDFKRFREIADKVGAYLMVDMAHIAGLVAAGLHPNPVPYADVVTTTTHKTLRG 232
[Close]
BLAST of EY659990 vs. ExPASy Swiss-Prot
Match: GLYA_PARD8 (Serine hydroxymethyltransferase OS=Parabacteroides distasonis (strain ATCC 8503 / DSM 20701 / NCTC 11152) GN=glyA PE=3 SV=1)

HSP 1 Score: 140.584 bits (353), Expect = 1.349e-36
Identity = 74/161 (45.96%), Postives = 101/161 (62.73%), Query Frame = 2 
Query:  239 DPEIADIIEHEKARQWKGLELIPSENFTSVSVMQAVGSVMTNNHSEGYPGARYYGGNEYIDMAESLCQKRALEAFRLDPEKWGVNVQSLSGSPSNFQVYTALLKPHDRIMALDLPHGGHLSHGYQTDTKKISAVSIFFETMPYRLNESTGYIDYDQLEKSA 721
            D  I DIIE E  RQ KG+ELI SENF S  VMQA+GS +TN ++EGYPG RYYGG E +D +E++  +R  + F  +   W  NVQ  SG+ +N  V+ A+L P D  + L+L HGGHLSHG       +++  + +    Y + E TG +DYDQ+E+ A
Sbjct:    4 DNIIFDIIEKEHQRQLKGIELIASENFVSDQVMQAMGSCLTNKYAEGYPGKRYYGGCEVVDQSETIAIERLKKLFNAE---W-ANVQPHSGAQANAAVFFAVLNPGDTFLGLNLSHGGHLSHG-----SPVNSSGVLYHATEYNVKEDTGRVDYDQMEEVA 155

HSP 2 Score: 34.2686 bits (77), Expect = 1.349e-36
Identity = 14/25 (56.00%), Postives = 21/25 (84.00%), Query Frame = 1 
Query:  736 KLIVAGASAYARLYDYERIRKVCNK 810
            KLIV G SAY+R +DY+R+R++ +K
Sbjct:  161 KLIVGGGSAYSRDWDYKRMREIADK 185
[Close]
BLAST of EY659990 vs. ExPASy Swiss-Prot
Match: GLYA_EUBR3 (Serine hydroxymethyltransferase OS=Eubacterium rectale (strain ATCC 33656 / VPI 0990) GN=glyA PE=3 SV=1)

HSP 1 Score: 137.887 bits (346), Expect = 2.291e-36
Identity = 75/161 (46.58%), Postives = 103/161 (63.98%), Query Frame = 2 
Query:  239 DPEIADIIEHEKARQWKGLELIPSENFTSVSVMQAVGSVMTNNHSEGYPGARYYGGNEYIDMAESLCQKRALEAFRLDPEKWGVNVQSLSGSPSNFQVYTALLKPHDRIMALDLPHGGHLSHGYQTDTKKISAVSIFFETMPYRLNESTGYIDYDQLEKSA 721
            DPEIA  I  E  RQ   +ELI SEN+ S +VM A+GS++TN ++EGYPG RYYGG E +D  E L ++RA E F  +     VNVQ  SG+ +N  V  A+LKP D IM ++L HGGHL+HG       ++    +F  +PY +N+  G+IDYD++E+ A
Sbjct:   11 DPEIASAITDEFERQNSHIELIASENWVSPAVMSAMGSILTNKYAEGYPGRRYYGGCECVDEVEELARERAKELFGAE----YVNVQPHSGAQANMAVQFAILKPGDTIMGMNLDHGGHLTHG-----SPVNFSGSYFHVVPYGVNDE-GFIDYDKVEEIA 161

HSP 2 Score: 36.1946 bits (82), Expect = 2.291e-36
Identity = 16/32 (50.00%), Postives = 24/32 (75.00%), Query Frame = 1 
Query:  715 KCHPLXXKLIVAGASAYARLYDYERIRKVCNK 810
            +C P   K+I+AGASAYAR  D++R R++ +K
Sbjct:  163 ECKP---KMIIAGASAYARTIDFKRFREIADK 191
[Close]
BLAST of EY659990 vs. ExPASy Swiss-Prot
Match: GLYA_CLOK5 (Serine hydroxymethyltransferase OS=Clostridium kluyveri (strain ATCC 8527 / DSM 555 / NCIMB 10680) GN=glyA PE=3 SV=1)

HSP 1 Score: 139.428 bits (350), Expect = 2.291e-36
Identity = 76/165 (46.06%), Postives = 101/165 (61.21%), Query Frame = 2 
Query:  227 LEVVDPEIADIIEHEKARQWKGLELIPSENFTSVSVMQAVGSVMTNNHSEGYPGARYYGGNEYIDMAESLCQKRALEAFRLDPEKWGVNVQSLSGSPSNFQVYTALLKPHDRIMALDLPHGGHLSHGYQTDTKKISAVSIFFETMPYRLNESTGYIDYDQLEKSA 721
            L+  D +I  IIE E  RQ  G+ELI SENFTS SVM+A+GS +TN ++EGYPG RYYGG   +D AE L + R  + F  +     VNVQ  SGS +N  VY ++LKP D ++ + L HGGHL+HG      K+S     +  + Y LN  T  IDYD++ + A
Sbjct:    6 LKNTDKDIYGIIEEEWERQKNGIELIASENFTSKSVMEAMGSFLTNKYAEGYPGKRYYGGCYIVDKAEDLARDRMKKLFNAE----HVNVQPHSGSQANMAVYMSVLKPGDTVLGMSLNHGGHLTHG-----SKVSFSGKLYNFVSYGLNSDTEIIDYDEMRELA 161

HSP 2 Score: 34.6538 bits (78), Expect = 2.291e-36
Identity = 13/25 (52.00%), Postives = 22/25 (88.00%), Query Frame = 1 
Query:  736 KLIVAGASAYARLYDYERIRKVCNK 810
            K+IV+GASAY R  D+++IR++C++
Sbjct:  167 KMIVSGASAYPRKIDFKKIREICDE 191
[Close]
BLAST of EY659990 vs. ExPASy Swiss-Prot
Match: GLYA_CLOK1 (Serine hydroxymethyltransferase OS=Clostridium kluyveri (strain NBRC 12016) GN=glyA PE=3 SV=1)

HSP 1 Score: 139.428 bits (350), Expect = 2.291e-36
Identity = 76/165 (46.06%), Postives = 101/165 (61.21%), Query Frame = 2 
Query:  227 LEVVDPEIADIIEHEKARQWKGLELIPSENFTSVSVMQAVGSVMTNNHSEGYPGARYYGGNEYIDMAESLCQKRALEAFRLDPEKWGVNVQSLSGSPSNFQVYTALLKPHDRIMALDLPHGGHLSHGYQTDTKKISAVSIFFETMPYRLNESTGYIDYDQLEKSA 721
            L+  D +I  IIE E  RQ  G+ELI SENFTS SVM+A+GS +TN ++EGYPG RYYGG   +D AE L + R  + F  +     VNVQ  SGS +N  VY ++LKP D ++ + L HGGHL+HG      K+S     +  + Y LN  T  IDYD++ + A
Sbjct:    6 LKNTDKDIYGIIEEEWERQKNGIELIASENFTSKSVMEAMGSFLTNKYAEGYPGKRYYGGCYIVDKAEDLARDRMKKLFNAE----HVNVQPHSGSQANMAVYMSVLKPGDTVLGMSLNHGGHLTHG-----SKVSFSGKLYNFVSYGLNSDTEIIDYDEMRELA 161

HSP 2 Score: 34.6538 bits (78), Expect = 2.291e-36
Identity = 13/25 (52.00%), Postives = 22/25 (88.00%), Query Frame = 1 
Query:  736 KLIVAGASAYARLYDYERIRKVCNK 810
            K+IV+GASAY R  D+++IR++C++
Sbjct:  167 KMIVSGASAYPRKIDFKKIREICDE 191
[Close]
BLAST of EY659990 vs. ExPASy Swiss-Prot
Match: GLYA_ACIC1 (Serine hydroxymethyltransferase OS=Acidothermus cellulolyticus (strain ATCC 43068 / 11B) GN=glyA PE=3 SV=1)

HSP 1 Score: 152.91 bits (385), Expect = 2.417e-36
Identity = 88/238 (36.97%), Postives = 130/238 (54.62%), Query Frame = 2 
Query:  221 APLEVVDPEIADIIEHEKARQWKGLELIPSENFTSVSVMQAVGSVMTNNHSEGYPGARYYGGNEYIDMAESLCQKRALEAFRLDPEKWGVNVQSLSGSPSNFQVYTALLKPHDRIMALDLPHGGHLSHGYQTDTKKISAVSIFFETMPYRLNESTGYIDYDQLEKSATLFXXNXXXXXXXXMHDCTIMSVFARFATN-KSYNVGRMAHLXWVGCCWCHPFTFENADGVTTTLTNTSWG 931
            A L   DP IAD+I  E+ RQ + + LIPSEN+ S +V++A G+V+TN +SEGYP  RYY G ++ID  E++  +RA + F +D      NVQ  SGSP+N  +Y ALL P D +M + LP GGHL+HG+      +SA  I+F ++ Y +   TG ID+D++ + A              +      + FA  A    +  V  +AH+  +     HP    +AD ++TT   T  G
Sbjct:    9 AALTATDPTIADLIRAEERRQSEKIRLIPSENYVSKAVLEATGTVLTNKYSEGYPNRRYYEGQQFIDQIETIAIERAKQLFGVD----HANVQPYSGSPANLAIYLALLSPGDTVMGMALPMGGHLTHGW-----PVSATGIWFRSVQYGVRRDTGRIDFDEVREVARRERPKVIFAGGTAIPRIIDFAAFAEIAREVNAVLVADIAHISGLVAGGVHPSPVGHADIISTTTHKTLRG 237
The following BLAST results are available for this feature:
BLAST of EY659990 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 500
Match NameE-valueIdentityDescription
GLYA_PELLD1.035e-3648.21Serine hydroxymethyltransferase OS=Pelodictyon lut... [more]
GLYA_BACFR1.036e-3647.83Serine hydroxymethyltransferase OS=Bacteroides fra... [more]
GLYA_BACFN1.036e-3647.83Serine hydroxymethyltransferase OS=Bacteroides fra... [more]
GLYA_THEPX1.085e-3639.66Serine hydroxymethyltransferase OS=Thermoanaerobac... [more]
GLYA_THEP31.085e-3639.66Serine hydroxymethyltransferase OS=Thermoanaerobac... [more]
GLYA_PARD81.349e-3645.96Serine hydroxymethyltransferase OS=Parabacteroides... [more]
GLYA_EUBR32.291e-3646.58Serine hydroxymethyltransferase OS=Eubacterium rec... [more]
GLYA_CLOK52.291e-3646.06Serine hydroxymethyltransferase OS=Clostridium klu... [more]
GLYA_CLOK12.291e-3646.06Serine hydroxymethyltransferase OS=Clostridium klu... [more]
GLYA_ACIC12.417e-3636.97Serine hydroxymethyltransferase OS=Acidothermus ce... [more]

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Properties
Property NameValue
Genbank descriptionCS00-C1-101-017-C10-CT.F Sweet orange leaf, infected with Xylella fastidiosa (stage 1 of 2) Citrus sinensis cDNA, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>EY659990 ID=EY659990; Name=EY659990; organism=Citrus sinensis; type=EST; length=932bp
ATGGACGCCTGAGGACCGGTCCGGAATTCTTCAACACCGGGCGCGTNCGG
NGAGAACTAACATAGAAAGGGCTCTGGNTCTTCGCAGGCTCTCATCTTCC
CTCGACAAACCCTCTCGTCCTCTCTTCAATGGCTCCTCCCTCTACTACAT
GTCATCTTTGCCAAATGAAGCTGTTTACGAGAAAGAGAAAAATGGTGTCA
CTTGGCCAAAACAACTGAATGCTCCACTTGAGGTCGTTGATCCTGAGATT
GCTGACATTATCGAGCATGAAAAAGCACGACAATGGAAGGGACTTGAACT
CATTCCTTCAGAGAACTTTACTTCAGTATCCGTGATGCAAGCTGTGGGGT
CTGTCATGACCAACAACCACAGTGAAGGATATCCAGGTGCAAGATATTAT
GGAGGAAACGAGTATATTGACATGGCGGAATCCTTATGTCAGAAGCGAGC
TCTGGAAGCATTTCGGTTGGATCCTGAAAAATGGGGAGTGAATGTGCAGT
CTCTGTCTGGATCTCCATCTAATTTTCAAGTCTACACTGCATTGTTAAAA
CCTCATGATAGGATCATGGCACTTGATCTTCCTCATGGTGGGCATCTCTC
ACACGGCTATCAGACAGATACCAAAAAGATATCTGCAGTGTCCATATTTT
TTGAGACAATGCCATACAGATTGAATGAGAGCACTGGCTATATAGACTAT
GACCAGTTGGAGAAAAGTGCCACCCTCTTCANGNCAAATTGATTGTTGCT
GGTGCTAGTGCTTATGCACGATTGTACGATTATGAGCGTATTCGCAAGGT
TTGCAACAAACAAAAGCTATAATGTTGGCAGAATGGCACACCTTAANTGG
GTTGGGTGCTGCTGGTGCCATCCCTTCACTTTTGAGAATGCAGATGGTGT
GACCACAACACTCACAAACACTTCCTGGGGCA
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