EY659881 | Citrus Genome Database

Overview


Name	EY659881
Unique Name	EY659881
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	925

Libraries


Library Name	Type
Sweet orange leaf, infected with Xylella fastidiosa (stage 1 of 2)	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Homology

BLAST of EY659881 vs. ExPASy Swiss-Prot
Match: GLGC_KLEP3 (Glucose-1-phosphate adenylyltransferase OS=Klebsiella pneumoniae (strain 342) GN=glgC PE=3 SV=1)

HSP 1 Score: 131.724 bits (330), Expect = 4.966e-31
Identity = 72/167 (43.11%), Postives = 100/167 (59.88%), Query Frame = 1

Query:  319 KNSQTCLDPEASRSVLGIILGGGAGTRLYPLTKKRAKPAVPLGANYRLIDIPVSNCLNSNISKIYVLTQFNSASLNRHLSRAYASNMGGYKNEGFVEVLAAQQSPEDPNWFQGTADAVRQYLWLFEEHNVLGILVLAGDHLYRMDYERFIQAHRETDADITVXALPM 819
            KN    L  +     + +IL GG GTRL  LT KRAKPAV  G  +R+ID  +SNC+NS I +I V+TQ+ S +L +H+ R + S      NE FV++L AQQ     NW++GTADAV Q L +   +    +++LAGDH+Y+ DY R +  H E  A  TV  +P+
Sbjct:    6 KNDPLMLARQLPIKSVALILAGGRGTRLKDLTIKRAKPAVHFGGKFRIIDFALSNCINSGIRRIGVITQYQSHTLVQHIQRGW-SFFSEEMNE-FVDLLPAQQRVHGENWYRGTADAVTQNLDIIRRYKAEYVVILAGDHIYKQDYSRMLIDHVEKGARCTVACMPV 170

HSP 2 Score: 24.6386 bits (52), Expect = 4.966e-31
Identity = 8/19 (42.11%), Postives = 15/19 (78.95%), Query Frame = 3

Query:  834 TAFGLMKIDEEGRLLNFLE 890
            +AFG+M +DE  +++ F+E
Sbjct:  176 SAFGVMAVDENEKIIEFVE 194

BLAST of EY659881 vs. ExPASy Swiss-Prot
Match: GLGC_FRATW (Glucose-1-phosphate adenylyltransferase OS=Francisella tularensis subsp. tularensis (strain WY96-3418) GN=glgC PE=3 SV=1)

HSP 1 Score: 130.183 bits (326), Expect = 4.967e-31
Identity = 66/150 (44.00%), Postives = 98/150 (65.33%), Query Frame = 1

Query:  355 RSVLGIILGGGAGTRLYPLTKKRAKPAVPLGANYRLIDIPVSNCLNSNISKIYVLTQFNSASLNRHLSRAYASNMGGYKNEGFVEVLAAQQSPEDPNWFQGTADAVRQYLWLFEEHNVLGILVLAGDHLYRMDYERFIQAHRETDADITV 804
            +  + ++L GG G+RLY LT  RAKPAV  G  +R+ID  +SNCLNS I +I V+TQ+ S SL RHL R +   + G  NE F+++L AQQ  ++ +W++GTADAV Q + +   +    ++VLAGDH+Y+MDY   ++ H ++    TV
Sbjct:   12 KKAMALVLAGGRGSRLYNLTDTRAKPAVYFGGKFRIIDFALSNCLNSGIRRIGVVTQYKSHSLLRHLQRGWGF-LRGELNE-FIDLLPAQQRVDEEHWYRGTADAVYQNIDILRSYGPEYVIVLAGDHIYKMDYSIMLRDHAQSGYKCTV 159

HSP 2 Score: 26.1794 bits (56), Expect = 4.967e-31
Identity = 10/22 (45.45%), Postives = 16/22 (72.73%), Query Frame = 3

Query:  837 AFGLMKIDEEGRLLNFLENQKE 902
            AFG+M IDE  ++ +F+E  K+
Sbjct:  171 AFGIMGIDENRKITSFIEKPKK 192

BLAST of EY659881 vs. ExPASy Swiss-Prot
Match: GLGC_FRATO (Glucose-1-phosphate adenylyltransferase OS=Francisella tularensis subsp. holarctica (strain OSU18) GN=glgC PE=3 SV=1)

HSP 1 Score: 130.183 bits (326), Expect = 4.967e-31
Identity = 66/150 (44.00%), Postives = 98/150 (65.33%), Query Frame = 1

Query:  355 RSVLGIILGGGAGTRLYPLTKKRAKPAVPLGANYRLIDIPVSNCLNSNISKIYVLTQFNSASLNRHLSRAYASNMGGYKNEGFVEVLAAQQSPEDPNWFQGTADAVRQYLWLFEEHNVLGILVLAGDHLYRMDYERFIQAHRETDADITV 804
            +  + ++L GG G+RLY LT  RAKPAV  G  +R+ID  +SNCLNS I +I V+TQ+ S SL RHL R +   + G  NE F+++L AQQ  ++ +W++GTADAV Q + +   +    ++VLAGDH+Y+MDY   ++ H ++    TV
Sbjct:   12 KKAMALVLAGGRGSRLYNLTDTRAKPAVYFGGKFRIIDFALSNCLNSGIRRIGVVTQYKSHSLLRHLQRGWGF-LRGELNE-FIDLLPAQQRVDEEHWYRGTADAVYQNIDILRSYGPEYVIVLAGDHIYKMDYSIMLRDHAQSGYKCTV 159

HSP 2 Score: 26.1794 bits (56), Expect = 4.967e-31
Identity = 10/22 (45.45%), Postives = 16/22 (72.73%), Query Frame = 3

Query:  837 AFGLMKIDEEGRLLNFLENQKE 902
            AFG+M IDE  ++ +F+E  K+
Sbjct:  171 AFGIMGIDENRKITSFIEKPKK 192

BLAST of EY659881 vs. ExPASy Swiss-Prot
Match: GLGC_FRATN (Glucose-1-phosphate adenylyltransferase OS=Francisella tularensis subsp. novicida (strain U112) GN=glgC PE=3 SV=1)

HSP 1 Score: 130.183 bits (326), Expect = 4.967e-31
Identity = 66/150 (44.00%), Postives = 98/150 (65.33%), Query Frame = 1

Query:  355 RSVLGIILGGGAGTRLYPLTKKRAKPAVPLGANYRLIDIPVSNCLNSNISKIYVLTQFNSASLNRHLSRAYASNMGGYKNEGFVEVLAAQQSPEDPNWFQGTADAVRQYLWLFEEHNVLGILVLAGDHLYRMDYERFIQAHRETDADITV 804
            +  + ++L GG G+RLY LT  RAKPAV  G  +R+ID  +SNCLNS I +I V+TQ+ S SL RHL R +   + G  NE F+++L AQQ  ++ +W++GTADAV Q + +   +    ++VLAGDH+Y+MDY   ++ H ++    TV
Sbjct:   12 KKAMALVLAGGRGSRLYNLTDTRAKPAVYFGGKFRIIDFALSNCLNSGIRRIGVVTQYKSHSLLRHLQRGWGF-LRGELNE-FIDLLPAQQRVDEEHWYRGTADAVYQNIDILRSYGPEYVIVLAGDHIYKMDYSIMLRDHAQSGYKCTV 159

HSP 2 Score: 26.1794 bits (56), Expect = 4.967e-31
Identity = 10/22 (45.45%), Postives = 16/22 (72.73%), Query Frame = 3

Query:  837 AFGLMKIDEEGRLLNFLENQKE 902
            AFG+M IDE  ++ +F+E  K+
Sbjct:  171 AFGIMGIDENRKITSFIEKPKK 192

BLAST of EY659881 vs. ExPASy Swiss-Prot
Match: GLGC_FRATM (Glucose-1-phosphate adenylyltransferase OS=Francisella tularensis subsp. mediasiatica (strain FSC147) GN=glgC PE=3 SV=1)

HSP 1 Score: 130.183 bits (326), Expect = 4.967e-31
Identity = 66/150 (44.00%), Postives = 98/150 (65.33%), Query Frame = 1

Query:  355 RSVLGIILGGGAGTRLYPLTKKRAKPAVPLGANYRLIDIPVSNCLNSNISKIYVLTQFNSASLNRHLSRAYASNMGGYKNEGFVEVLAAQQSPEDPNWFQGTADAVRQYLWLFEEHNVLGILVLAGDHLYRMDYERFIQAHRETDADITV 804
            +  + ++L GG G+RLY LT  RAKPAV  G  +R+ID  +SNCLNS I +I V+TQ+ S SL RHL R +   + G  NE F+++L AQQ  ++ +W++GTADAV Q + +   +    ++VLAGDH+Y+MDY   ++ H ++    TV
Sbjct:   12 KKAMALVLAGGRGSRLYNLTDTRAKPAVYFGGKFRIIDFALSNCLNSGIRRIGVVTQYKSHSLLRHLQRGWGF-LRGELNE-FIDLLPAQQRVDEEHWYRGTADAVYQNIDILRSYGPEYVIVLAGDHIYKMDYSIMLRDHAQSGYKCTV 159

HSP 2 Score: 26.1794 bits (56), Expect = 4.967e-31
Identity = 10/22 (45.45%), Postives = 16/22 (72.73%), Query Frame = 3

Query:  837 AFGLMKIDEEGRLLNFLENQKE 902
            AFG+M IDE  ++ +F+E  K+
Sbjct:  171 AFGIMGIDENRKITSFIEKPKK 192

BLAST of EY659881 vs. ExPASy Swiss-Prot
Match: GLGC_FRATH (Glucose-1-phosphate adenylyltransferase OS=Francisella tularensis subsp. holarctica (strain LVS) GN=glgC PE=3 SV=1)

HSP 1 Score: 130.183 bits (326), Expect = 4.967e-31
Identity = 66/150 (44.00%), Postives = 98/150 (65.33%), Query Frame = 1

Query:  355 RSVLGIILGGGAGTRLYPLTKKRAKPAVPLGANYRLIDIPVSNCLNSNISKIYVLTQFNSASLNRHLSRAYASNMGGYKNEGFVEVLAAQQSPEDPNWFQGTADAVRQYLWLFEEHNVLGILVLAGDHLYRMDYERFIQAHRETDADITV 804
            +  + ++L GG G+RLY LT  RAKPAV  G  +R+ID  +SNCLNS I +I V+TQ+ S SL RHL R +   + G  NE F+++L AQQ  ++ +W++GTADAV Q + +   +    ++VLAGDH+Y+MDY   ++ H ++    TV
Sbjct:   12 KKAMALVLAGGRGSRLYNLTDTRAKPAVYFGGKFRIIDFALSNCLNSGIRRIGVVTQYKSHSLLRHLQRGWGF-LRGELNE-FIDLLPAQQRVDEEHWYRGTADAVYQNIDILRSYGPEYVIVLAGDHIYKMDYSIMLRDHAQSGYKCTV 159

HSP 2 Score: 26.1794 bits (56), Expect = 4.967e-31
Identity = 10/22 (45.45%), Postives = 16/22 (72.73%), Query Frame = 3

Query:  837 AFGLMKIDEEGRLLNFLENQKE 902
            AFG+M IDE  ++ +F+E  K+
Sbjct:  171 AFGIMGIDENRKITSFIEKPKK 192

BLAST of EY659881 vs. ExPASy Swiss-Prot
Match: GLGC_FRATF (Glucose-1-phosphate adenylyltransferase OS=Francisella tularensis subsp. holarctica (strain FTNF002-00 / FTA) GN=glgC PE=3 SV=1)

HSP 1 Score: 130.183 bits (326), Expect = 4.967e-31
Identity = 66/150 (44.00%), Postives = 98/150 (65.33%), Query Frame = 1

Query:  355 RSVLGIILGGGAGTRLYPLTKKRAKPAVPLGANYRLIDIPVSNCLNSNISKIYVLTQFNSASLNRHLSRAYASNMGGYKNEGFVEVLAAQQSPEDPNWFQGTADAVRQYLWLFEEHNVLGILVLAGDHLYRMDYERFIQAHRETDADITV 804
            +  + ++L GG G+RLY LT  RAKPAV  G  +R+ID  +SNCLNS I +I V+TQ+ S SL RHL R +   + G  NE F+++L AQQ  ++ +W++GTADAV Q + +   +    ++VLAGDH+Y+MDY   ++ H ++    TV
Sbjct:   12 KKAMALVLAGGRGSRLYNLTDTRAKPAVYFGGKFRIIDFALSNCLNSGIRRIGVVTQYKSHSLLRHLQRGWGF-LRGELNE-FIDLLPAQQRVDEEHWYRGTADAVYQNIDILRSYGPEYVIVLAGDHIYKMDYSIMLRDHAQSGYKCTV 159

HSP 2 Score: 26.1794 bits (56), Expect = 4.967e-31
Identity = 10/22 (45.45%), Postives = 16/22 (72.73%), Query Frame = 3

Query:  837 AFGLMKIDEEGRLLNFLENQKE 902
            AFG+M IDE  ++ +F+E  K+
Sbjct:  171 AFGIMGIDENRKITSFIEKPKK 192

BLAST of EY659881 vs. ExPASy Swiss-Prot
Match: GLGC_SHEB9 (Glucose-1-phosphate adenylyltransferase OS=Shewanella baltica (strain OS195) GN=glgC PE=3 SV=1)

HSP 1 Score: 130.568 bits (327), Expect = 4.967e-31
Identity = 71/156 (45.51%), Postives = 99/156 (63.46%), Query Frame = 1

Query:  352 SRSVLGIILGGGAGTRLYPLTKKRAKPAVPLGANYRLIDIPVSNCLNSNISKIYVLTQFNSASLNRHLSRAYASNMGGYKNE--GFVEVLAAQQSPEDPNWFQGTADAVRQYLWLFEEHNVLGILVLAGDHLYRMDYERFIQAHRETDADITVXAL 813
            +R    +IL GG G+RL+ LT  RAKPA+  G  +R+ID P+SNC+NS I ++ V+TQ+ S SL RH+ R +    G +K E    VE+L A Q   + NW+QGTADAV Q + +        ++VL+GDH+YRMDY   + AH E+ AD+TV  L
Sbjct:   11 TRETYALILAGGRGSRLHELTDWRAKPALYFGGKFRIIDFPLSNCINSGIRRVGVVTQYKSHSLIRHVMRGW----GHFKKELGESVEILPASQRYSE-NWYQGTADAVFQNIDIIRHELPKYVMVLSGDHVYRMDYAGLLAAHAESGADMTVSCL 161

HSP 2 Score: 25.7942 bits (55), Expect = 4.967e-31
Identity = 10/18 (55.56%), Postives = 15/18 (83.33%), Query Frame = 3

Query:  837 AFGLMKIDEEGRLLNFLE 890
            +FG+M++DEE R+L F E
Sbjct:  171 SFGVMEVDEEMRILGFEE 188

BLAST of EY659881 vs. ExPASy Swiss-Prot
Match: GLGC_DEIRA (Glucose-1-phosphate adenylyltransferase OS=Deinococcus radiodurans GN=glgC PE=3 SV=2)

HSP 1 Score: 134.806 bits (338), Expect = 4.969e-31
Identity = 65/158 (41.14%), Postives = 101/158 (63.92%), Query Frame = 1

Query:  361 VLGIILGGGAGTRLYPLTKKRAKPAVPLGANYRLIDIPVSNCLNSNISKIYVLTQFNSASLNRHLSRAYASNMGGYKNEGFVEVLAAQQ---SPEDPNWFQGTADAVRQYLWLFEEHNVLGILVLAGDHLYRMDYERFIQAHRETDADITVXALPMDE 825
            VLG+IL GG G+RL PLT+KR+KPAVP G+ YR+ID  ++N +NS +  +YVLTQ+ + SL  H+ R +    G + ++ F+ ++ AQ          W++GTADAV Q + L +      + + +GDH+Y+M+ E  ++ H ET AD+T+ A PM +
Sbjct:    5 VLGMILAGGQGSRLAPLTQKRSKPAVPFGSKYRIIDFAINNFINSGMFSVYVLTQYKAQSLTEHIQRGW--RFGTFLSDYFITLVPAQMYRFEELGDAWYRGTADAVYQNMHLIDNFEADYVAIFSGDHIYKMNVEHMLEKHIETRADVTIAAYPMPQ 160

HSP 2 Score: 21.557 bits (44), Expect = 4.969e-31
Identity = 8/17 (47.06%), Postives = 12/17 (70.59%), Query Frame = 3

Query:  840 FGLMKIDEEGRLLNFLE 890
            FG+M++DE  R+  F E
Sbjct:  166 FGVMQVDERWRVTEFHE 182

BLAST of EY659881 vs. ExPASy Swiss-Prot
Match: GLGC_LACLM (Glucose-1-phosphate adenylyltransferase OS=Lactococcus lactis subsp. cremoris (strain MG1363) GN=glgC PE=3 SV=1)

HSP 1 Score: 135.191 bits (339), Expect = 5.162e-31
Identity = 68/157 (43.31%), Postives = 99/157 (63.06%), Query Frame = 1

Query:  361 VLGIILGGGAGTRLYPLTKKRAKPAVPLGANYRLIDIPVSNCLNSNISKIYVLTQFNSASLNRHLSRAYASNMGGYKNEGFVEVLAAQQSPEDPNWFQGTADAVRQYLWLFEEHNVLGILVLAGDHLYRMDYERFIQAHRETDADITVXAL--PMDE 825
            +LG+IL GG GTRL  LTK  AKPAVP G  YR+ID  +SNC NSN+  + V+TQ+   +LN H+       + G  N G V +L    S E   WF+GT+ AV Q +   ++ N   +L+L+GDH+Y+MDYE  +++H+E +A +TV  +  P++E
Sbjct:    5 MLGLILAGGQGTRLGKLTKDVAKPAVPFGGRYRIIDFALSNCANSNVKNVGVITQYQPLTLNAHIGNGAPWGLNGI-NRG-VTILQPYFSQEGSKWFEGTSHAVYQNISYIDQQNPEYVLILSGDHIYKMDYEAMLESHKEREASLTVSVMEVPLEE 159

The following BLAST results are available for this feature:

BLAST of EY659881 vs. ExPASy Swiss-Prot

BLAST of EY659881 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 307


Match Name	E-value	Identity	Description
GLGC_KLEP3	4.966e-31	43.11	Glucose-1-phosphate adenylyltransferase OS=Klebsie...	[more]
GLGC_FRATW	4.967e-31	44.00	Glucose-1-phosphate adenylyltransferase OS=Francis...	[more]
GLGC_FRATO	4.967e-31	44.00	Glucose-1-phosphate adenylyltransferase OS=Francis...	[more]
GLGC_FRATN	4.967e-31	44.00	Glucose-1-phosphate adenylyltransferase OS=Francis...	[more]
GLGC_FRATM	4.967e-31	44.00	Glucose-1-phosphate adenylyltransferase OS=Francis...	[more]
GLGC_FRATH	4.967e-31	44.00	Glucose-1-phosphate adenylyltransferase OS=Francis...	[more]
GLGC_FRATF	4.967e-31	44.00	Glucose-1-phosphate adenylyltransferase OS=Francis...	[more]
GLGC_SHEB9	4.967e-31	45.51	Glucose-1-phosphate adenylyltransferase OS=Shewane...	[more]
GLGC_DEIRA	4.969e-31	41.14	Glucose-1-phosphate adenylyltransferase OS=Deinoco...	[more]
GLGC_LACLM	5.162e-31	43.31	Glucose-1-phosphate adenylyltransferase OS=Lactoco...	[more]

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Properties


Property Name	Value
Genbank description	CS00-C1-101-016-B05-CT.F Sweet orange leaf, infected with Xylella fastidiosa (stage 1 of 2) Citrus sinensis cDNA, mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>EY659881 ID=EY659881; Name=EY659881; organism=Citrus sinensis; type=EST; length=925bp
CAACAACACAGGCGTCCGACTCTCACTTTACANAATAACTCAGGCTCAGC
TCGCTTAACGATTACTGTTAACGTTAACAGATTGTACTCATTAATGGCGA
GTATGGCGTCGATCGGATCGCTCAAAGTTCCGTCATCTCCGTCAACTGCA
GCAACGTCGTCTAACTCCAACAATCACAGCCGCAGGAGTGTGGTTAAACG
ACTGGCGTTCTCGTCGTCTCAGCTCTCCGGTGACAAGATTTTTTCTAAAG
CTGTCACCGGTGATCGGCGAAGGGAGAGGCGTCCGATCGTTGTGTCTCCT
CAAGCGGTGTCGGATTCGAAAAATTCTCAGACTTGCCTTGATCCTGAAGC
CAGTCGAAGTGTTTTGGGGATTATACTTGGAGGTGGAGCAGGGACTCGCT
TGTATCCACTTACGAAGAAGAGGGCAAAGCCTGCTGTTCCATTGGGAGCT
AATTACAGGCTGATTGATATTCCTGTGAGCAATTGTTTGAATAGTAACAT
TTCAAAGATCTATGTTCTCACTCAGTTCAATTCTGCGTCCCTGAATCGTC
ACCTATCGAGGGCGTATGCTAGCAACATGGGTGGTTACAAGAACGAAGGA
TTTGTTGAAGTTCTTGCTGCACAGCAGAGTCCAGAAGATCCCAATTGGTT
TCAGGGTACAGCTGATGCTGTGAGGCAGTATTTGTGGCTGTTTGAGGAGC
ACAATGTTCTGGGAATTCTCGTTCTTGCTGGTGATCATTTGTACCGAATG
GACTATGAGAGATTTATTCAGGCACACAGAGAAACTGATGCGGATATCAC
AGTANCTGCCCTGCCCATGGATGAAAAAGGCCTACTGCTTTTGGNCTGAT
GAAAATCGATGAAGAGGGACGCCTATTGAATTTTCTGGAGAACCAAAAGG
AGACACTTTAAGGTATGAAGGGTGA