EY659861 | Citrus Genome Database

Overview


Name	EY659861
Unique Name	EY659861
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	886

Libraries


Library Name	Type
Sweet orange leaf, infected with Xylella fastidiosa (stage 1 of 2)	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Homology

BLAST of EY659861 vs. ExPASy Swiss-Prot
Match: GLGA_STRZT (Glycogen synthase OS=Streptococcus pneumoniae (strain Taiwan19F-14) GN=glgA PE=3 SV=1)

HSP 1 Score: 127.487 bits (319), Expect = 1.351e-30
Identity = 75/201 (37.31%), Postives = 104/201 (51.74%), Query Frame = 1

Query:  124 MDAKPLLKEALQAEVGLPVDRNIPVIGFIGRLEEQKGSDILAAAIPHFIKENVQIIVLGTGKKPMEKQLEQLEILYPEKARGVAKFNIPLAHMIIAGADFILIPSRFEPCGLIQLHAMRYGTVPIVASTGGLVDTV------EEGFTGFQMGSFSVDCEAVDPVDVAAVSTTVRRALATYGT--QALAEMMKNGMAQDLSW 702
            +  K   K  LQ  VGLPV  ++P++G + RL  QKG D++  ++ H ++E+VQI++LGTG    E        +YP+K      F++ LA  I A  D  L+PSRFEPCGL Q+ AMRYGT+P+V   GGL DTV      E   TGF          + D +    ++ T + AL  Y         + K  M  D SW
Sbjct:  271 LSGKAKNKAKLQERVGLPVRADVPLVGIVSRLTRQKGFDVVVESLHHILQEDVQIVLLGTGDPAFEGAFSWFAQIYPDKLSTNITFDVKLAQEIYAACDLFLMPSRFEPCGLSQMMAMRYGTLPLVHEVGGLRDTVRAFNPIEGSGTGF----------SFDNLSPYWLNWTFQTALDLYRNHPDIWRNLQKQAMESDFSW 461

HSP 2 Score: 27.335 bits (59), Expect = 1.351e-30
Identity = 10/19 (52.63%), Postives = 14/19 (73.68%), Query Frame = 2

Query:   32 RIKGIVNGMDVQEWNPLTD 88
            ++ GIVNG+D   +NP TD
Sbjct:  241 KVSGIVNGIDADLYNPQTD 259

BLAST of EY659861 vs. ExPASy Swiss-Prot
Match: GLGA_STRZP (Glycogen synthase OS=Streptococcus pneumoniae (strain P1031) GN=glgA PE=3 SV=1)

HSP 1 Score: 127.487 bits (319), Expect = 1.351e-30
Identity = 75/201 (37.31%), Postives = 104/201 (51.74%), Query Frame = 1

Query:  124 MDAKPLLKEALQAEVGLPVDRNIPVIGFIGRLEEQKGSDILAAAIPHFIKENVQIIVLGTGKKPMEKQLEQLEILYPEKARGVAKFNIPLAHMIIAGADFILIPSRFEPCGLIQLHAMRYGTVPIVASTGGLVDTV------EEGFTGFQMGSFSVDCEAVDPVDVAAVSTTVRRALATYGT--QALAEMMKNGMAQDLSW 702
            +  K   K  LQ  VGLPV  ++P++G + RL  QKG D++  ++ H ++E+VQI++LGTG    E        +YP+K      F++ LA  I A  D  L+PSRFEPCGL Q+ AMRYGT+P+V   GGL DTV      E   TGF          + D +    ++ T + AL  Y         + K  M  D SW
Sbjct:  271 LSGKAKNKAKLQERVGLPVRADVPLVGIVSRLTRQKGFDVVVESLHHILQEDVQIVLLGTGDPAFEGAFSWFAQIYPDKLSTNITFDVKLAQEIYAACDLFLMPSRFEPCGLSQMMAMRYGTLPLVHEVGGLRDTVRAFNPIEGSGTGF----------SFDNLSPYWLNWTFQTALDLYRNHPDIWRNLQKQAMESDFSW 461

HSP 2 Score: 27.335 bits (59), Expect = 1.351e-30
Identity = 10/19 (52.63%), Postives = 14/19 (73.68%), Query Frame = 2

Query:   32 RIKGIVNGMDVQEWNPLTD 88
            ++ GIVNG+D   +NP TD
Sbjct:  241 KVSGIVNGIDADLYNPQTD 259

BLAST of EY659861 vs. ExPASy Swiss-Prot
Match: GLGA_STRR6 (Glycogen synthase OS=Streptococcus pneumoniae (strain ATCC BAA-255 / R6) GN=glgA PE=3 SV=1)

HSP 1 Score: 127.487 bits (319), Expect = 1.351e-30
Identity = 75/201 (37.31%), Postives = 104/201 (51.74%), Query Frame = 1

Query:  124 MDAKPLLKEALQAEVGLPVDRNIPVIGFIGRLEEQKGSDILAAAIPHFIKENVQIIVLGTGKKPMEKQLEQLEILYPEKARGVAKFNIPLAHMIIAGADFILIPSRFEPCGLIQLHAMRYGTVPIVASTGGLVDTV------EEGFTGFQMGSFSVDCEAVDPVDVAAVSTTVRRALATYGT--QALAEMMKNGMAQDLSW 702
            +  K   K  LQ  VGLPV  ++P++G + RL  QKG D++  ++ H ++E+VQI++LGTG    E        +YP+K      F++ LA  I A  D  L+PSRFEPCGL Q+ AMRYGT+P+V   GGL DTV      E   TGF          + D +    ++ T + AL  Y         + K  M  D SW
Sbjct:  271 LSGKAKNKAKLQERVGLPVRADVPLVGIVSRLTRQKGFDVVVESLHHILQEDVQIVLLGTGDPAFEGAFSWFAQIYPDKLSTNITFDVKLAQEIYAACDLFLMPSRFEPCGLSQMMAMRYGTLPLVHEVGGLRDTVRAFNPIEGSGTGF----------SFDNLSPYWLNWTFQTALDLYRNHPDIWRNLQKQAMESDFSW 461

HSP 2 Score: 27.335 bits (59), Expect = 1.351e-30
Identity = 10/19 (52.63%), Postives = 14/19 (73.68%), Query Frame = 2

Query:   32 RIKGIVNGMDVQEWNPLTD 88
            ++ GIVNG+D   +NP TD
Sbjct:  241 KVSGIVNGIDADLYNPQTD 259

BLAST of EY659861 vs. ExPASy Swiss-Prot
Match: GLGA_STRPS (Glycogen synthase OS=Streptococcus pneumoniae (strain CGSP14) GN=glgA PE=3 SV=1)

HSP 1 Score: 127.487 bits (319), Expect = 1.351e-30
Identity = 75/201 (37.31%), Postives = 104/201 (51.74%), Query Frame = 1

Query:  124 MDAKPLLKEALQAEVGLPVDRNIPVIGFIGRLEEQKGSDILAAAIPHFIKENVQIIVLGTGKKPMEKQLEQLEILYPEKARGVAKFNIPLAHMIIAGADFILIPSRFEPCGLIQLHAMRYGTVPIVASTGGLVDTV------EEGFTGFQMGSFSVDCEAVDPVDVAAVSTTVRRALATYGT--QALAEMMKNGMAQDLSW 702
            +  K   K  LQ  VGLPV  ++P++G + RL  QKG D++  ++ H ++E+VQI++LGTG    E        +YP+K      F++ LA  I A  D  L+PSRFEPCGL Q+ AMRYGT+P+V   GGL DTV      E   TGF          + D +    ++ T + AL  Y         + K  M  D SW
Sbjct:  271 LSGKAKNKAKLQERVGLPVRADVPLVGIVSRLTRQKGFDVVVESLHHILQEDVQIVLLGTGDPAFEGAFSWFAQIYPDKLSTNITFDVKLAQEIYAACDLFLMPSRFEPCGLSQMMAMRYGTLPLVHEVGGLRDTVRAFNPIEGSGTGF----------SFDNLSPYWLNWTFQTALDLYRNHPDIWRNLQKQAMESDFSW 461

HSP 2 Score: 27.335 bits (59), Expect = 1.351e-30
Identity = 10/19 (52.63%), Postives = 14/19 (73.68%), Query Frame = 2

Query:   32 RIKGIVNGMDVQEWNPLTD 88
            ++ GIVNG+D   +NP TD
Sbjct:  241 KVSGIVNGIDADLYNPQTD 259

BLAST of EY659861 vs. ExPASy Swiss-Prot
Match: GLGA_STRPN (Glycogen synthase OS=Streptococcus pneumoniae GN=glgA PE=3 SV=1)

HSP 1 Score: 127.487 bits (319), Expect = 1.351e-30
Identity = 75/201 (37.31%), Postives = 104/201 (51.74%), Query Frame = 1

Query:  124 MDAKPLLKEALQAEVGLPVDRNIPVIGFIGRLEEQKGSDILAAAIPHFIKENVQIIVLGTGKKPMEKQLEQLEILYPEKARGVAKFNIPLAHMIIAGADFILIPSRFEPCGLIQLHAMRYGTVPIVASTGGLVDTV------EEGFTGFQMGSFSVDCEAVDPVDVAAVSTTVRRALATYGT--QALAEMMKNGMAQDLSW 702
            +  K   K  LQ  VGLPV  ++P++G + RL  QKG D++  ++ H ++E+VQI++LGTG    E        +YP+K      F++ LA  I A  D  L+PSRFEPCGL Q+ AMRYGT+P+V   GGL DTV      E   TGF          + D +    ++ T + AL  Y         + K  M  D SW
Sbjct:  271 LSGKAKNKAKLQERVGLPVRADVPLVGIVSRLTRQKGFDVVVESLHHILQEDVQIVLLGTGDPAFEGAFSWFAQIYPDKLSTNITFDVKLAQEIYAACDLFLMPSRFEPCGLSQMMAMRYGTLPLVHEVGGLRDTVRAFNPIEGSGTGF----------SFDNLSPYWLNWTFQTALDLYRNHPDIWRNLQKQAMESDFSW 461

HSP 2 Score: 27.335 bits (59), Expect = 1.351e-30
Identity = 10/19 (52.63%), Postives = 14/19 (73.68%), Query Frame = 2

Query:   32 RIKGIVNGMDVQEWNPLTD 88
            ++ GIVNG+D   +NP TD
Sbjct:  241 KVSGIVNGIDADLYNPQTD 259

BLAST of EY659861 vs. ExPASy Swiss-Prot
Match: GLGA_STRP7 (Glycogen synthase OS=Streptococcus pneumoniae (strain 70585) GN=glgA PE=3 SV=1)

HSP 1 Score: 127.487 bits (319), Expect = 1.351e-30
Identity = 75/201 (37.31%), Postives = 104/201 (51.74%), Query Frame = 1

Query:  124 MDAKPLLKEALQAEVGLPVDRNIPVIGFIGRLEEQKGSDILAAAIPHFIKENVQIIVLGTGKKPMEKQLEQLEILYPEKARGVAKFNIPLAHMIIAGADFILIPSRFEPCGLIQLHAMRYGTVPIVASTGGLVDTV------EEGFTGFQMGSFSVDCEAVDPVDVAAVSTTVRRALATYGT--QALAEMMKNGMAQDLSW 702
            +  K   K  LQ  VGLPV  ++P++G + RL  QKG D++  ++ H ++E+VQI++LGTG    E        +YP+K      F++ LA  I A  D  L+PSRFEPCGL Q+ AMRYGT+P+V   GGL DTV      E   TGF          + D +    ++ T + AL  Y         + K  M  D SW
Sbjct:  271 LSGKAKNKAKLQERVGLPVRADVPLVGIVSRLTRQKGFDVVVESLHHILQEDVQIVLLGTGDPAFEGAFSWFAQIYPDKLSTNITFDVKLAQEIYAACDLFLMPSRFEPCGLSQMMAMRYGTLPLVHEVGGLRDTVRAFNPIEGSGTGF----------SFDNLSPYWLNWTFQTALDLYRNHPDIWRNLQKQAMESDFSW 461

HSP 2 Score: 27.335 bits (59), Expect = 1.351e-30
Identity = 10/19 (52.63%), Postives = 14/19 (73.68%), Query Frame = 2

Query:   32 RIKGIVNGMDVQEWNPLTD 88
            ++ GIVNG+D   +NP TD
Sbjct:  241 KVSGIVNGIDADLYNPQTD 259

BLAST of EY659861 vs. ExPASy Swiss-Prot
Match: GLGA_STRP2 (Glycogen synthase OS=Streptococcus pneumoniae serotype 2 (strain D39 / NCTC 7466) GN=glgA PE=3 SV=1)

HSP 1 Score: 127.487 bits (319), Expect = 1.351e-30
Identity = 75/201 (37.31%), Postives = 104/201 (51.74%), Query Frame = 1

Query:  124 MDAKPLLKEALQAEVGLPVDRNIPVIGFIGRLEEQKGSDILAAAIPHFIKENVQIIVLGTGKKPMEKQLEQLEILYPEKARGVAKFNIPLAHMIIAGADFILIPSRFEPCGLIQLHAMRYGTVPIVASTGGLVDTV------EEGFTGFQMGSFSVDCEAVDPVDVAAVSTTVRRALATYGT--QALAEMMKNGMAQDLSW 702
            +  K   K  LQ  VGLPV  ++P++G + RL  QKG D++  ++ H ++E+VQI++LGTG    E        +YP+K      F++ LA  I A  D  L+PSRFEPCGL Q+ AMRYGT+P+V   GGL DTV      E   TGF          + D +    ++ T + AL  Y         + K  M  D SW
Sbjct:  271 LSGKAKNKAKLQERVGLPVRADVPLVGIVSRLTRQKGFDVVVESLHHILQEDVQIVLLGTGDPAFEGAFSWFAQIYPDKLSTNITFDVKLAQEIYAACDLFLMPSRFEPCGLSQMMAMRYGTLPLVHEVGGLRDTVRAFNPIEGSGTGF----------SFDNLSPYWLNWTFQTALDLYRNHPDIWRNLQKQAMESDFSW 461

HSP 2 Score: 27.335 bits (59), Expect = 1.351e-30
Identity = 10/19 (52.63%), Postives = 14/19 (73.68%), Query Frame = 2

Query:   32 RIKGIVNGMDVQEWNPLTD 88
            ++ GIVNG+D   +NP TD
Sbjct:  241 KVSGIVNGIDADLYNPQTD 259

BLAST of EY659861 vs. ExPASy Swiss-Prot
Match: GLGA_LACS1 (Glycogen synthase OS=Lactobacillus salivarius subsp. salivarius (strain UCC118) GN=glgA PE=3 SV=1)

HSP 1 Score: 126.716 bits (317), Expect = 1.351e-30
Identity = 77/198 (38.89%), Postives = 108/198 (54.55%), Query Frame = 1

Query:  145 KEALQAEVGLPVDRNIPVIGFIGRLEEQKGSDILAAAIPHFIKENVQIIVLGTGKKPMEKQLEQLEILYPEKARGVAKFNIPLAHMIIAGADFILIPSRFEPCGLIQLHAMRYGTVPIVASTGGLVDTVE-------EGFTGFQMGSFSVDCEAVDPVDVAAVSTTVRRALATYG-TQALAEMMKNGMAQDLSWKGPS 714
            K ALQ E GLP  +++PVI  + RL  QKG  ++ + + + ++ +VQ+IVLGTG    E         YP+K      F++ LA  I AGAD  L+PS FEPCGL Q+ +MRYGT+PIV   GGL D+V+       EG TGF    FS             +   ++ AL  Y  + A  +++K  M+QD SW+  S
Sbjct:  279 KLALQKEFGLPQRKDVPVIAMVSRLTAQKGFQLVVSEMQNLVQFDVQVIVLGTGDANFEHDFRYFADTYPDKVGAAITFDVGLAQRIYAGADMFLMPSAFEPCGLSQMISMRYGTLPIVHQIGGLQDSVQPFNPVTGEG-TGFGFHDFS----------GFYMMQEIQEALRLYAESVAWTKVVKQAMSQDFSWETAS 465

HSP 2 Score: 28.1054 bits (61), Expect = 1.351e-30
Identity = 11/26 (42.31%), Postives = 19/26 (73.08%), Query Frame = 2

Query:   29 YRIKGIVNGMDVQEWNPLTDHI*VSN 106
            ++++GI+NG+D +  NP TD   V+N
Sbjct:  241 WKLRGILNGIDYERNNPATDKNLVAN 266

BLAST of EY659861 vs. ExPASy Swiss-Prot
Match: GLGA2_BRAJA (Glycogen synthase 2 OS=Bradyrhizobium japonicum GN=glgA2 PE=3 SV=1)

HSP 1 Score: 124.79 bits (312), Expect = 1.758e-30
Identity = 74/194 (38.14%), Postives = 111/194 (57.22%), Query Frame = 1

Query:  145 KEALQAEVGLPVDRNIPVIGFIGRLEEQKGSDILAAAIPHFIKENVQIIVLGTGKKPMEKQLEQLEILYPEKARGVAKFNIPLAHMIIAGADFILIPSRFEPCGLIQLHAMRYGTVPIVASTGGLVDTV----EEG--FTGFQMGSFSVDCEAVDPVDVAAVSTTVRRA-LATYGTQALAEMMKNGMAQDLSWK 705
            K  LQ +  L      P++G I RL  QKG D+L  AIP  + E +Q+ +LG+G + ++ + +      P +   V  ++  LAH+I AG+D +++PSRFEPCGL QL A+RYG VPIV+  GGL DT+    E G   TGF+ G          PV   A++ T+R+A  A +      ++ +NG+A D+SW+
Sbjct:  280 KAVLQQQFNLDSSDEAPLLGVISRLSWQKGLDLLLEAIPTILGEGMQLALLGSGDRDLQDRYQAAARANPGRIGVVIGYDEILAHLIQAGSDALIVPSRFEPCGLTQLCALRYGAVPIVSRVGGLEDTIVDIGEAGREATGFKFG----------PVTADALAGTLRKANTAFHDKLTWRQLQRNGLATDVSWR 463

HSP 2 Score: 29.6462 bits (65), Expect = 1.758e-30
Identity = 10/18 (55.56%), Postives = 14/18 (77.78%), Query Frame = 2

Query:   35 IKGIVNGMDVQEWNPLTD 88
            + GI+NG+D+  WNP TD
Sbjct:  244 LSGILNGIDIAVWNPQTD 261

BLAST of EY659861 vs. ExPASy Swiss-Prot
Match: GLGA_NITMU (Glycogen synthase OS=Nitrosospira multiformis (strain ATCC 25196 / NCIMB 11849) GN=glgA PE=3 SV=1)

HSP 1 Score: 133.265 bits (334), Expect = 1.846e-30
Identity = 83/236 (35.17%), Postives = 127/236 (53.81%), Query Frame = 1

Query:   76 PLD*PYIGVKYDASTVMDAKPLLKEALQAEVGLPVDRNIPVIGFIGRLEEQKGSDILAAAIPHFIKENVQIIVLGTGKKPMEKQLEQLEILYPEKARGVAKFNIPLAHMIIAGADFILIPSRFEPCGLIQLHAMRYGTVPIVASTGGLVDTVEEGFTGFQMGSFSVDCEAVDPVDVAAVS------------TTVRRALATYGTQALAE-MMKNGMAQDLSWKGPS*KWXETLLNL 744
            P++ P++   Y     +  K + K ALQ ++GL VD ++P+ G + RL  QKG D+L   I   I    Q+++LG+G+  +E++L ++   +P K      F+  LAH+I AGAD  L+PSRFEPCGL Q+++  YGT P+V  TGGL+DTV             VDC A    D  A                + RA+A Y  + + + +M+NGM QD SW+  +  + +  L+L
Sbjct:  270 PVNDPHLEQNYSMDD-LSGKVINKAALQQQLGLTVDPDVPLFGAVSRLTYQKGYDLLLRIITQLIDIPGQLVILGSGETMLEQELMRMARNHPGKMAVRIGFDEKLAHLIEAGADCFLMPSRFEPCGLNQMYSQCYGTPPLVHGTGGLLDTV-------------VDCTAESLADGTATGFVFHELTPEAFLGALERAVAAYRDKPVWDRLMRNGMVQDFSWRASATDYRKIYLSL 491

The following BLAST results are available for this feature:

BLAST of EY659861 vs. ExPASy Swiss-Prot

BLAST of EY659861 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 364


Match Name	E-value	Identity	Description
GLGA_STRZT	1.351e-30	37.31	Glycogen synthase OS=Streptococcus pneumoniae (str...	[more]
GLGA_STRZP	1.351e-30	37.31	Glycogen synthase OS=Streptococcus pneumoniae (str...	[more]
GLGA_STRR6	1.351e-30	37.31	Glycogen synthase OS=Streptococcus pneumoniae (str...	[more]
GLGA_STRPS	1.351e-30	37.31	Glycogen synthase OS=Streptococcus pneumoniae (str...	[more]
GLGA_STRPN	1.351e-30	37.31	Glycogen synthase OS=Streptococcus pneumoniae GN=g...	[more]
GLGA_STRP7	1.351e-30	37.31	Glycogen synthase OS=Streptococcus pneumoniae (str...	[more]
GLGA_STRP2	1.351e-30	37.31	Glycogen synthase OS=Streptococcus pneumoniae sero...	[more]
GLGA_LACS1	1.351e-30	38.89	Glycogen synthase OS=Lactobacillus salivarius subs...	[more]
GLGA2_BRAJA	1.758e-30	38.14	Glycogen synthase 2 OS=Bradyrhizobium japonicum GN...	[more]
GLGA_NITMU	1.846e-30	35.17	Glycogen synthase OS=Nitrosospira multiformis (str...	[more]

Pages

Properties


Property Name	Value
Genbank description	CS00-C1-101-015-H08-CT.F Sweet orange leaf, infected with Xylella fastidiosa (stage 1 of 2) Citrus sinensis cDNA, mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>EY659861 ID=EY659861; Name=EY659861; organism=Citrus sinensis; type=EST; length=886bp
TTCGAAAAACCACGCGTCCGATCCGTAATACCGCATTAAGGGAATTGTGA
ACGGGATGGATGTCCAGGAGTGGAACCCCTTGACTGACCATATATAGGTG
TCAAATATGATGCTTCAACTGTGATGGATGCGAAGCCTCTTCTGAAGGAA
GCCCTCCAAGCAGAAGTTGGGTTGCCTGTCGATAGAAATATTCCTGTCAT
AGGCTTCATTGGTAGACTTGAAGAGCAGAAAGGCTCAGATATTCTTGCAG
CTGCCATTCCCCATTTCATCAAAGAGAATGTTCAGATAATAGTCCTTGGT
ACTGGCAAAAAACCAATGGAGAAGCAGCTTGAACAGTTGGAGATACTGTA
CCCTGAGAAAGCCAGAGGAGTAGCGAAATTCAATATTCCTCTGGCGCATA
TGATAATAGCAGGAGCTGATTTTATTTTGATTCCAAGCAGATTTGAACCT
TGTGGTCTCATTCAATTACATGCCATGCGTTATGGAACCGTACCTATCGT
GGCTTCCACTGGTGGTTTGGTTGACACTGTGGAAGAAGGCTTTACAGGAT
TCCAGATGGGAAGCTTCAGTGTTGACTGTGAGGCTGTGGATCCAGTAGAT
GTGGCTGCAGTGTCCACAACCGTCAGAAGAGCTCTTGCAACCTATGGTAC
TCAAGCTTTGGCTGAAATGATGAAAAATGGCATGGCTCAAGATCTTTCAT
GGAAAGGACCCAGCTAGAAATGGGANGAGACCCTACTTAACCTGGAAAGT
GCTGGTAGTGAACCTGGAATTGACNGCGAAGAAATTGCTCCTCTTTGCAA
GAAAATGTGGCCACTCCTTGATCGAGGCTACAAAATCTCCACTTTTATTT
GGACCGCAACATGTTCCTGTAATTTCTCACACCCNA