EY659470 | Citrus Genome Database

Overview


Name	EY659470
Unique Name	EY659470
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	917

Libraries


Library Name	Type
Sweet orange leaf, infected with Xylella fastidiosa (stage 1 of 2)	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Homology

BLAST of EY659470 vs. ExPASy Swiss-Prot
Match: TDH_PECCP (L-threonine 3-dehydrogenase OS=Pectobacterium carotovorum subsp. carotovorum (strain PC1) GN=tdh PE=3 SV=1)

HSP 1 Score: 89.3521 bits (220), Expect = 3.228e-17
Identity = 53/159 (33.33%), Postives = 84/159 (52.83%), Query Frame = 1

Query:   28 GSLPNQVVHPADLCFKLPDNVSLEEGAMCEPLSVGLHACRRANIGPETNVLIMGAGPIGLVTMLGARAFGAPRIVIVDVDDYRLSVAKELGADNIVKVS-TNLQDIAEEVEKIQKAMGTGIDVSFDCAGFNKTMSTALSATRAGGKVCLVGMGHHEMTV 501
            GS    +V PA   F++PDN+S E  A+ +P    +H     ++  E +VLI GAGPIG++     R  GA  +VI DV+ YRL +A+++GA   V V+   L D+      I+  M  G D+  + +G        L A   GG++ ++G+ H  M++
Sbjct:  119 GSFAEYLVIPAYNAFRIPDNISDELAAIFDPFGNAVHTALSFDLVGE-DVLIAGAGPIGMMAAAVCRHVGARNVVITDVNAYRLDLARKMGATRAVNVAEERLADV-----MIELGMTEGFDIGLEMSGAPSAFRAMLKAMNHGGRIAMLGIPHEPMSI 271

BLAST of EY659470 vs. ExPASy Swiss-Prot
Match: TDH_SHIFL (L-threonine 3-dehydrogenase OS=Shigella flexneri GN=tdh PE=3 SV=1)

HSP 1 Score: 87.0409 bits (214), Expect = 3.419e-17
Identity = 51/162 (31.48%), Postives = 86/162 (53.09%), Query Frame = 1

Query:   28 GSLPNQVVHPADLCFKLPDNVSLEEGAMCEPLSVGLHACRRANIGPETNVLIMGAGPIGLVTMLGARAFGAPRIVIVDVDDYRLSVAKELGADNIVKVS-TNLQDIAEEVEKIQKAMGTGIDVSFDCAGFNKTMSTALSATRAGGKVCLVGMGHHEMTVPLT 510
            G     +V PA   FK+PDN+S +  ++ +P    +H     ++  E +VL+ GAGPIG++    A+  GA  +VI DV++YRL +A+++G    V V+  NL D+  E+      M  G DV  + +G      T L     GG++ ++G+   +M++  T
Sbjct:  119 GCFAEYLVIPAFNAFKIPDNISDDLASIFDPFGNAVHTALSFDLVGE-DVLVSGAGPIGIMAAAVAKHVGARNVVITDVNEYRLELARKMGITRAVNVAKENLNDVMAEL-----GMTEGFDVGLEMSGAPPAFRTMLDTMNHGGRIAMLGIPPSDMSIDWT 274

HSP 2 Score: 22.7126 bits (47), Expect = 3.419e-17
Identity = 10/23 (43.48%), Postives = 14/23 (60.87%), Query Frame = 2

Query:  563 TWPLCLEFLXSGKIDVKPLITHR 631
            TW      + SG +D+ P+ITHR
Sbjct:  294 TWYKMAALIQSG-LDLSPIITHR 315

BLAST of EY659470 vs. ExPASy Swiss-Prot
Match: TDH_SHIF8 (L-threonine 3-dehydrogenase OS=Shigella flexneri serotype 5b (strain 8401) GN=tdh PE=3 SV=1)

HSP 1 Score: 87.0409 bits (214), Expect = 3.419e-17
Identity = 51/162 (31.48%), Postives = 86/162 (53.09%), Query Frame = 1

Query:   28 GSLPNQVVHPADLCFKLPDNVSLEEGAMCEPLSVGLHACRRANIGPETNVLIMGAGPIGLVTMLGARAFGAPRIVIVDVDDYRLSVAKELGADNIVKVS-TNLQDIAEEVEKIQKAMGTGIDVSFDCAGFNKTMSTALSATRAGGKVCLVGMGHHEMTVPLT 510
            G     +V PA   FK+PDN+S +  ++ +P    +H     ++  E +VL+ GAGPIG++    A+  GA  +VI DV++YRL +A+++G    V V+  NL D+  E+      M  G DV  + +G      T L     GG++ ++G+   +M++  T
Sbjct:  119 GCFAEYLVIPAFNAFKIPDNISDDLASIFDPFGNAVHTALSFDLVGE-DVLVSGAGPIGIMAAAVAKHVGARNVVITDVNEYRLELARKMGITRAVNVAKENLNDVMAEL-----GMTEGFDVGLEMSGAPPAFRTMLDTMNHGGRIAMLGIPPSDMSIDWT 274

HSP 2 Score: 22.7126 bits (47), Expect = 3.419e-17
Identity = 10/23 (43.48%), Postives = 14/23 (60.87%), Query Frame = 2

Query:  563 TWPLCLEFLXSGKIDVKPLITHR 631
            TW      + SG +D+ P+ITHR
Sbjct:  294 TWYKMAALIQSG-LDLSPIITHR 315

BLAST of EY659470 vs. ExPASy Swiss-Prot
Match: TDH_SHIBS (L-threonine 3-dehydrogenase OS=Shigella boydii serotype 4 (strain Sb227) GN=tdh PE=3 SV=1)

HSP 1 Score: 87.0409 bits (214), Expect = 3.419e-17
Identity = 51/162 (31.48%), Postives = 86/162 (53.09%), Query Frame = 1

Query:   28 GSLPNQVVHPADLCFKLPDNVSLEEGAMCEPLSVGLHACRRANIGPETNVLIMGAGPIGLVTMLGARAFGAPRIVIVDVDDYRLSVAKELGADNIVKVS-TNLQDIAEEVEKIQKAMGTGIDVSFDCAGFNKTMSTALSATRAGGKVCLVGMGHHEMTVPLT 510
            G     +V PA   FK+PDN+S +  ++ +P    +H     ++  E +VL+ GAGPIG++    A+  GA  +VI DV++YRL +A+++G    V V+  NL D+  E+      M  G DV  + +G      T L     GG++ ++G+   +M++  T
Sbjct:  119 GCFAEYLVIPAFNAFKIPDNISDDLASIFDPFGNAVHTALSFDLVGE-DVLVSGAGPIGIMAAAVAKHVGARNVVITDVNEYRLELARKMGITRAVNVAKENLNDVMAEL-----GMTEGFDVGLEMSGAPPAFRTMLDTMNHGGRIAMLGIPPSDMSIDWT 274

HSP 2 Score: 22.7126 bits (47), Expect = 3.419e-17
Identity = 10/23 (43.48%), Postives = 14/23 (60.87%), Query Frame = 2

Query:  563 TWPLCLEFLXSGKIDVKPLITHR 631
            TW      + SG +D+ P+ITHR
Sbjct:  294 TWYKMAALIQSG-LDLSPIITHR 315

BLAST of EY659470 vs. ExPASy Swiss-Prot
Match: TDH_SHIB3 (L-threonine 3-dehydrogenase OS=Shigella boydii serotype 18 (strain CDC 3083-94 / BS512) GN=tdh PE=3 SV=1)

HSP 1 Score: 87.0409 bits (214), Expect = 3.419e-17
Identity = 51/162 (31.48%), Postives = 86/162 (53.09%), Query Frame = 1

Query:   28 GSLPNQVVHPADLCFKLPDNVSLEEGAMCEPLSVGLHACRRANIGPETNVLIMGAGPIGLVTMLGARAFGAPRIVIVDVDDYRLSVAKELGADNIVKVS-TNLQDIAEEVEKIQKAMGTGIDVSFDCAGFNKTMSTALSATRAGGKVCLVGMGHHEMTVPLT 510
            G     +V PA   FK+PDN+S +  ++ +P    +H     ++  E +VL+ GAGPIG++    A+  GA  +VI DV++YRL +A+++G    V V+  NL D+  E+      M  G DV  + +G      T L     GG++ ++G+   +M++  T
Sbjct:  119 GCFAEYLVIPAFNAFKIPDNISDDLASIFDPFGNAVHTALSFDLVGE-DVLVSGAGPIGIMAAAVAKHVGARNVVITDVNEYRLELARKMGITRAVNVAKENLNDVMAEL-----GMTEGFDVGLEMSGAPPAFRTMLDTMNHGGRIAMLGIPPSDMSIDWT 274

HSP 2 Score: 22.7126 bits (47), Expect = 3.419e-17
Identity = 10/23 (43.48%), Postives = 14/23 (60.87%), Query Frame = 2

Query:  563 TWPLCLEFLXSGKIDVKPLITHR 631
            TW      + SG +D+ P+ITHR
Sbjct:  294 TWYKMAALIQSG-LDLSPIITHR 315

BLAST of EY659470 vs. ExPASy Swiss-Prot
Match: TDH_ECOUT (L-threonine 3-dehydrogenase OS=Escherichia coli (strain UTI89 / UPEC) GN=tdh PE=3 SV=1)

HSP 1 Score: 87.0409 bits (214), Expect = 3.419e-17
Identity = 51/162 (31.48%), Postives = 86/162 (53.09%), Query Frame = 1

Query:   28 GSLPNQVVHPADLCFKLPDNVSLEEGAMCEPLSVGLHACRRANIGPETNVLIMGAGPIGLVTMLGARAFGAPRIVIVDVDDYRLSVAKELGADNIVKVS-TNLQDIAEEVEKIQKAMGTGIDVSFDCAGFNKTMSTALSATRAGGKVCLVGMGHHEMTVPLT 510
            G     +V PA   FK+PDN+S +  ++ +P    +H     ++  E +VL+ GAGPIG++    A+  GA  +VI DV++YRL +A+++G    V V+  NL D+  E+      M  G DV  + +G      T L     GG++ ++G+   +M++  T
Sbjct:  119 GCFAEYLVIPAFNAFKIPDNISDDLASIFDPFGNAVHTALSFDLVGE-DVLVSGAGPIGIMAAAVAKHVGARNVVITDVNEYRLELARKMGITRAVNVAKENLNDVMTEL-----GMTEGFDVGLEMSGAPPAFRTMLDTMNHGGRIAMLGIPPSDMSIDWT 274

HSP 2 Score: 22.7126 bits (47), Expect = 3.419e-17
Identity = 10/23 (43.48%), Postives = 14/23 (60.87%), Query Frame = 2

Query:  563 TWPLCLEFLXSGKIDVKPLITHR 631
            TW      + SG +D+ P+ITHR
Sbjct:  294 TWYKMAALIQSG-LDLSPIITHR 315

BLAST of EY659470 vs. ExPASy Swiss-Prot
Match: TDH_ECOSM (L-threonine 3-dehydrogenase OS=Escherichia coli (strain SMS-3-5 / SECEC) GN=tdh PE=3 SV=1)

HSP 1 Score: 87.0409 bits (214), Expect = 3.419e-17
Identity = 51/162 (31.48%), Postives = 86/162 (53.09%), Query Frame = 1

Query:   28 GSLPNQVVHPADLCFKLPDNVSLEEGAMCEPLSVGLHACRRANIGPETNVLIMGAGPIGLVTMLGARAFGAPRIVIVDVDDYRLSVAKELGADNIVKVS-TNLQDIAEEVEKIQKAMGTGIDVSFDCAGFNKTMSTALSATRAGGKVCLVGMGHHEMTVPLT 510
            G     +V PA   FK+PDN+S +  ++ +P    +H     ++  E +VL+ GAGPIG++    A+  GA  +VI DV++YRL +A+++G    V V+  NL D+  E+      M  G DV  + +G      T L     GG++ ++G+   +M++  T
Sbjct:  119 GCFAEYLVIPAFNAFKIPDNISDDLASIFDPFGNAVHTALSFDLVGE-DVLVSGAGPIGIMAAAVAKHVGARNVVITDVNEYRLELARKMGITRAVNVAKENLNDVMTEL-----GMTEGFDVGLEMSGAPPAFRTMLDTMNHGGRIAMLGIPPSDMSIDWT 274

HSP 2 Score: 22.7126 bits (47), Expect = 3.419e-17
Identity = 10/23 (43.48%), Postives = 14/23 (60.87%), Query Frame = 2

Query:  563 TWPLCLEFLXSGKIDVKPLITHR 631
            TW      + SG +D+ P+ITHR
Sbjct:  294 TWYKMAALIQSG-LDLSPIITHR 315

BLAST of EY659470 vs. ExPASy Swiss-Prot
Match: TDH_ECOSE (L-threonine 3-dehydrogenase OS=Escherichia coli (strain SE11) GN=tdh PE=3 SV=1)

HSP 1 Score: 87.0409 bits (214), Expect = 3.419e-17
Identity = 51/162 (31.48%), Postives = 86/162 (53.09%), Query Frame = 1

Query:   28 GSLPNQVVHPADLCFKLPDNVSLEEGAMCEPLSVGLHACRRANIGPETNVLIMGAGPIGLVTMLGARAFGAPRIVIVDVDDYRLSVAKELGADNIVKVS-TNLQDIAEEVEKIQKAMGTGIDVSFDCAGFNKTMSTALSATRAGGKVCLVGMGHHEMTVPLT 510
            G     +V PA   FK+PDN+S +  ++ +P    +H     ++  E +VL+ GAGPIG++    A+  GA  +VI DV++YRL +A+++G    V V+  NL D+  E+      M  G DV  + +G      T L     GG++ ++G+   +M++  T
Sbjct:  119 GCFAEYLVIPAFNAFKIPDNISDDLASIFDPFGNAVHTALSFDLVGE-DVLVSGAGPIGIMAAAVAKHVGARNVVITDVNEYRLELARKMGITRAVNVAKENLNDVMAEL-----GMTEGFDVGLEMSGAPPAFRTMLDTMNHGGRIAMLGIPPSDMSIDWT 274

HSP 2 Score: 22.7126 bits (47), Expect = 3.419e-17
Identity = 10/23 (43.48%), Postives = 14/23 (60.87%), Query Frame = 2

Query:  563 TWPLCLEFLXSGKIDVKPLITHR 631
            TW      + SG +D+ P+ITHR
Sbjct:  294 TWYKMAALIQSG-LDLSPIITHR 315

BLAST of EY659470 vs. ExPASy Swiss-Prot
Match: TDH_ECOLC (L-threonine 3-dehydrogenase OS=Escherichia coli (strain ATCC 8739 / DSM 1576 / Crooks) GN=tdh PE=3 SV=1)

HSP 1 Score: 87.0409 bits (214), Expect = 3.419e-17
Identity = 51/162 (31.48%), Postives = 86/162 (53.09%), Query Frame = 1

Query:   28 GSLPNQVVHPADLCFKLPDNVSLEEGAMCEPLSVGLHACRRANIGPETNVLIMGAGPIGLVTMLGARAFGAPRIVIVDVDDYRLSVAKELGADNIVKVS-TNLQDIAEEVEKIQKAMGTGIDVSFDCAGFNKTMSTALSATRAGGKVCLVGMGHHEMTVPLT 510
            G     +V PA   FK+PDN+S +  ++ +P    +H     ++  E +VL+ GAGPIG++    A+  GA  +VI DV++YRL +A+++G    V V+  NL D+  E+      M  G DV  + +G      T L     GG++ ++G+   +M++  T
Sbjct:  119 GCFAEYLVIPAFNAFKIPDNISDDLASIFDPFGNAVHTALSFDLVGE-DVLVSGAGPIGIMAAAVAKHVGARNVVITDVNEYRLELARKMGITRAVNVAKENLNDVMAEL-----GMTEGFDVGLEMSGAPPAFRTMLDTMNHGGRIAMLGIPPSDMSIDWT 274

HSP 2 Score: 22.7126 bits (47), Expect = 3.419e-17
Identity = 10/23 (43.48%), Postives = 14/23 (60.87%), Query Frame = 2

Query:  563 TWPLCLEFLXSGKIDVKPLITHR 631
            TW      + SG +D+ P+ITHR
Sbjct:  294 TWYKMAALIQSG-LDLSPIITHR 315

BLAST of EY659470 vs. ExPASy Swiss-Prot
Match: TDH_ECOL6 (L-threonine 3-dehydrogenase OS=Escherichia coli O6 GN=tdh PE=3 SV=1)

HSP 1 Score: 87.0409 bits (214), Expect = 3.419e-17
Identity = 51/162 (31.48%), Postives = 86/162 (53.09%), Query Frame = 1

Query:   28 GSLPNQVVHPADLCFKLPDNVSLEEGAMCEPLSVGLHACRRANIGPETNVLIMGAGPIGLVTMLGARAFGAPRIVIVDVDDYRLSVAKELGADNIVKVS-TNLQDIAEEVEKIQKAMGTGIDVSFDCAGFNKTMSTALSATRAGGKVCLVGMGHHEMTVPLT 510
            G     +V PA   FK+PDN+S +  ++ +P    +H     ++  E +VL+ GAGPIG++    A+  GA  +VI DV++YRL +A+++G    V V+  NL D+  E+      M  G DV  + +G      T L     GG++ ++G+   +M++  T
Sbjct:  119 GCFAEYLVIPAFNAFKIPDNISDDLASIFDPFGNAVHTALSFDLVGE-DVLVSGAGPIGIMAAAVAKHVGARNVVITDVNEYRLELARKMGITRAVNVAKENLNDVMTEL-----GMTEGFDVGLEMSGAPPAFRTMLDTMNHGGRIAMLGIPPSDMSIDWT 274

HSP 2 Score: 22.7126 bits (47), Expect = 3.419e-17
Identity = 10/23 (43.48%), Postives = 14/23 (60.87%), Query Frame = 2

Query:  563 TWPLCLEFLXSGKIDVKPLITHR 631
            TW      + SG +D+ P+ITHR
Sbjct:  294 TWYKMAALIQSG-LDLSPIITHR 315

The following BLAST results are available for this feature:

BLAST of EY659470 vs. ExPASy Swiss-Prot

BLAST of EY659470 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 209


Match Name	E-value	Identity	Description
TDH_PECCP	3.228e-17	33.33	L-threonine 3-dehydrogenase OS=Pectobacterium caro...	[more]
TDH_SHIFL	3.419e-17	31.48	L-threonine 3-dehydrogenase OS=Shigella flexneri G...	[more]
TDH_SHIF8	3.419e-17	31.48	L-threonine 3-dehydrogenase OS=Shigella flexneri s...	[more]
TDH_SHIBS	3.419e-17	31.48	L-threonine 3-dehydrogenase OS=Shigella boydii ser...	[more]
TDH_SHIB3	3.419e-17	31.48	L-threonine 3-dehydrogenase OS=Shigella boydii ser...	[more]
TDH_ECOUT	3.419e-17	31.48	L-threonine 3-dehydrogenase OS=Escherichia coli (s...	[more]
TDH_ECOSM	3.419e-17	31.48	L-threonine 3-dehydrogenase OS=Escherichia coli (s...	[more]
TDH_ECOSE	3.419e-17	31.48	L-threonine 3-dehydrogenase OS=Escherichia coli (s...	[more]
TDH_ECOLC	3.419e-17	31.48	L-threonine 3-dehydrogenase OS=Escherichia coli (s...	[more]
TDH_ECOL6	3.419e-17	31.48	L-threonine 3-dehydrogenase OS=Escherichia coli O6...	[more]

Pages

Properties


Property Name	Value
Genbank description	CS00-C1-101-009-E04-CT.F Sweet orange leaf, infected with Xylella fastidiosa (stage 1 of 2) Citrus sinensis cDNA, mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>EY659470 ID=EY659470; Name=EY659470; organism=Citrus sinensis; type=EST; length=917bp
AAATTTTTTTTTTCTCCGCCCGTTCATGGTTCCCTGCCTAATCAGGTGGT
GCATCCTGCAGACCTATGTTTTAAGCTGCCGGACAATGTGAGCCTGGAGG
AAGGGGCTATGTGTGAGCCCTTGAGTGTTGGTCTTCATGCCTGCCGCCGT
GCCAATATCGGTCCTGAGACAAATGTGCTTATCATGGGAGCAGGGCCCAT
AGGACTTGTCACAATGCTAGGAGCTCGTGCTTTTGGAGCTCCTAGAATTG
TCATTGTGGATGTGGATGACTATCGTTTATCTGTTGCAAAGGAACTTGGT
GCAGATAATATTGTTAAAGTTTCAACAAATTTGCAGGACATAGCTGAAGA
AGTGGAAAAGATACAGAAAGCCATGGGGACAGGAATAGATGTGAGCTTTG
ATTGTGCAGGCTTTAACAAAACCATGTCAACAGCACTGAGTGCTACTCGT
GCAGGTGGTAAAGTTTGCCTCGTGGGGATGGGTCATCATGAAATGACTGT
CCCACTCACTCCGGCTGCTGCAAGGGATGTGGATGTGGTGGGGGGTGTTT
CGCTACATGAACACCTGGCCACTGTGTCTGGAGTTCCTTANAAGCGGAAA
GATTGATGTAAAGCCCCTTATCACGCATAGGGTTTGGTTTCTNCCAGAAA
GAAGTGGAAGAAGGCCTTGAAACAGTGCTCGTGGTGGTACTGGCATCAAA
GTCATGTTTAATCTGTAAATACAACGTAAGTAAACGTTTGGGCAAAGATG
AAATAAAATGAATTGCCGCCGAGTATATGAATAAAAGGGTAGCTATACTT
ATTTCTATCTATTACAAAATAAATCAGATTTCTCAAAAAAAAAAAAAAGG
CCGGCCGCTTATAAGATACAGCTTATACCCTGGCTGCAACGTTTAACTTT
TTATATGGCACCTAATT