EY659414

Overview
NameEY659414
Unique NameEY659414
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length822
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
BLAST of EY659414 vs. ExPASy Swiss-Prot
Match: RBS2_CHLRE (Ribulose bisphosphate carboxylase small chain 2, chloroplastic OS=Chlamydomonas reinhardtii GN=RBCS-2 PE=1 SV=1)

HSP 1 Score: 139.813 bits (351), Expect = 1.748e-32
Identity = 78/179 (43.58%), Postives = 101/179 (56.42%), Query Frame = 3
Query:   57 SSTTVATANRASLAQASMVAPFTGLKSSSAFPATKKTNNDITSIASNGGRVQCMKVWPPTGLKKFETLSYLPPLSDEALLKEISYLLRSGWIPCLEF-ELEKGWVYREHHSSPG-----YYDGRYWTMWKLPMYGCTDATQVLKEVGEVQKEYPHSFVRIIGFDNKRQVQCISFLAAKP 575
            SS + A A  A     S V P   LK     PA K       + A+       M VW P   K FET SYLPPLSDE +  ++ Y++ +GWIPCLEF E +K +V  E     G     YYD RYWTMWKLPM+GC D  QVL+E+    K +P ++VR++ FDN++QVQ + FL  +P
Sbjct:    8 SSVSAAVARPAR----SSVRPMAALK-----PAVKAAPVAAPAQANQ------MMVWTPVNNKMFETFSYLPPLSDEQIAAQVDYIVANGWIPCLEFAESDKAYVSNESAIRFGSVSCLYYDNRYWTMWKLPMFGCRDPMQVLREIVACTKAFPDAYVRLVAFDNQKQVQIMGFLVQRP 171          
BLAST of EY659414 vs. ExPASy Swiss-Prot
Match: RBS_SINAL (Ribulose bisphosphate carboxylase small chain (Fragment) OS=Sinapis alba GN=RBCS PE=3 SV=1)

HSP 1 Score: 139.428 bits (350), Expect = 2.284e-32
Identity = 60/77 (77.92%), Postives = 70/77 (90.91%), Query Frame = 3
Query:  348 ELEKGWVYREHHSSPGYYDGRYWTMWKLPMYGCTDATQVLKEVGEVQKEYPHSFVRIIGFDNKRQVQCISFLAAKPP 578
            ELE G+VYREH S+PGYYDGRYWTMWKLP++GCTD+ QVLKEV E +KEYP++F+RIIGFDN RQVQCISF+A KPP
Sbjct:    1 ELEHGFVYREHGSTPGYYDGRYWTMWKLPLFGCTDSAQVLKEVQECKKEYPNAFIRIIGFDNNRQVQCISFIAYKPP 77          
BLAST of EY659414 vs. ExPASy Swiss-Prot
Match: RBS_CHLMO (Ribulose bisphosphate carboxylase small chain, chloroplastic OS=Chlamydomonas moewusii GN=RBCS PE=2 SV=1)

HSP 1 Score: 139.428 bits (350), Expect = 2.284e-32
Identity = 59/125 (47.20%), Postives = 84/125 (67.20%), Query Frame = 3
Query:  219 KVWPPTGLKKFETLSYLPPLSDEALLKEISYLLRSGWIPCLEFE------LEKGWVYREHHSSPGYYDGRYWTMWKLPMYGCTDATQVLKEVGEVQKEYPHSFVRIIGFDNKRQVQCISFLAAKP 575
            KVW P   K++ET SYLPPL+++ + +++ Y++ +GW PCLEF       +      R    S GYYD RYWTMWKLPM+GCTD +QVL+EV   Q  +P+ ++R++ FDN +QVQC+ FL  +P
Sbjct:   30 KVWQPVNNKQYETFSYLPPLTNQKIGRQVDYIINNGWTPCLEFADPSTSFVSNANAVRLQGVSAGYYDNRYWTMWKLPMFGCTDPSQVLREVSACQVAFPNVYIRLVAFDNVKQVQCMGFLVQRP 154          
BLAST of EY659414 vs. ExPASy Swiss-Prot
Match: RBS1_CHLRE (Ribulose bisphosphate carboxylase small chain 1, chloroplastic OS=Chlamydomonas reinhardtii GN=RBCS-1 PE=1 SV=1)

HSP 1 Score: 139.043 bits (349), Expect = 2.982e-32
Identity = 77/179 (43.02%), Postives = 101/179 (56.42%), Query Frame = 3
Query:   57 SSTTVATANRASLAQASMVAPFTGLKSSSAFPATKKTNNDITSIASNGGRVQCMKVWPPTGLKKFETLSYLPPLSDEALLKEISYLLRSGWIPCLEF-ELEKGWVYREHHSSPG-----YYDGRYWTMWKLPMYGCTDATQVLKEVGEVQKEYPHSFVRIIGFDNKRQVQCISFLAAKP 575
            SS + A A  A     S V P   LK     PA K       + A+       M VW P   K FET SYLPPL+DE +  ++ Y++ +GWIPCLEF E +K +V  E     G     YYD RYWTMWKLPM+GC D  QVL+E+    K +P ++VR++ FDN++QVQ + FL  +P
Sbjct:    8 SSVSAAVARPAR----SSVRPMAALK-----PAVKAAPVAAPAQANQ------MMVWTPVNNKMFETFSYLPPLTDEQIAAQVDYIVANGWIPCLEFAEADKAYVSNESAIRFGSVSCLYYDNRYWTMWKLPMFGCRDPMQVLREIVACTKAFPDAYVRLVAFDNQKQVQIMGFLVQRP 171          
BLAST of EY659414 vs. ExPASy Swiss-Prot
Match: RBS3_ACECL (Ribulose bisphosphate carboxylase small chain 3, chloroplastic OS=Acetabularia cliftonii GN=RBCS-3 PE=2 SV=1)

HSP 1 Score: 134.806 bits (338), Expect = 5.625e-31
Identity = 68/152 (44.74%), Postives = 89/152 (58.55%), Query Frame = 3
Query:  147 FPATKKTNNDITSIASNGGRVQC--MKVWPPTGLKKFETLSYLPPLSDEALLKEISYLLRSGWIPCLEFEL-EKGWVYREHHSSPG-----YYDGRYWTMWKLPMYGCTDATQVLKEVGEVQKEYPHSFVRIIGFDNKRQVQCISFLAAKPP 578
            FP   K N      ASN    +C  M VW P   K FET SYLPPL+DE + K++ Y+L + W PCLEF   ++ +   E+    G     Y D RYWTMWKLPM+GCTD +QVL E+    K +P +++R++ FD  RQVQ   FL  +PP
Sbjct:   22 FPRATKNNK---GFASNAAVQKCRDMMVWQPFNNKMFETFSYLPPLTDEQISKQVDYILANSWTPCLEFAASDQAYAGNENCIRMGPVSSTYQDNRYWTMWKLPMFGCTDGSQVLSEIQACTKAFPDAYIRLVCFDANRQVQISGFLVHRPP 170          
BLAST of EY659414 vs. ExPASy Swiss-Prot
Match: RBS4_ACECL (Ribulose bisphosphate carboxylase small chain 4, chloroplastic OS=Acetabularia cliftonii GN=RBCS-4 PE=2 SV=1)

HSP 1 Score: 131.724 bits (330), Expect = 4.761e-30
Identity = 67/157 (42.68%), Postives = 88/157 (56.05%), Query Frame = 3
Query:  126 GLKSSSAFPATKKTNNDITSIASNGGRVQCMKVWPPTGLKKFETLSYLPPLSDEALLKEISYLLRSGWIPCLEFEL-EKGWVYREHHSSPG-----YYDGRYWTMWKLPMYGCTDATQVLKEVGEVQKEYPHSFVRIIGFDNKRQVQCISFLAAKPP 578
            G    SA P          + A N  R   M VW P   K FET SYLPPL+DE + K++ Y+L + W PCLEF   ++ +   E+    G     Y D RYWTMWKLPM+GCTD +QVL E+    K +P +++R++ FD  RQVQ   FL  +PP
Sbjct:   15 GCTKPSAVPKVSINRKGFLNTAMNKKRE--MMVWQPFNNKMFETFSYLPPLTDEQISKQVDYILANSWTPCLEFAASDQAYAGNENCIRMGPVASTYQDNRYWTMWKLPMFGCTDGSQVLSEIQACTKAFPDAYIRLVCFDANRQVQISGFLVHRPP 169          
BLAST of EY659414 vs. ExPASy Swiss-Prot
Match: RBS5_ACECL (Ribulose bisphosphate carboxylase small chain 5, chloroplastic OS=Acetabularia cliftonii GN=RBCS-5 PE=2 SV=1)

HSP 1 Score: 131.339 bits (329), Expect = 6.219e-30
Identity = 69/171 (40.35%), Postives = 95/171 (55.56%), Query Frame = 3
Query:   87 ASLAQASMVAPFTGLKSSSAFPATKKTNNDITS-IASNGGRVQCMKVWPPTGLKKFETLSYLPPLSDEALLKEISYLLRSGWIPCLEFEL-EKGWVYREHHSSPG-----YYDGRYWTMWKLPMYGCTDATQVLKEVGEVQKEYPHSFVRIIGFDNKRQVQCISFLAAKPP 578
            A++   ++V    G   SS+ P          S +A    R   M VW P   K FET SYLPPL+DE + K++ Y+L + W PCLEF   ++ +   E+    G     Y D RYWTMWKLPM+GCTD +QVL E+    K +P +++R++ FD  RQVQ   FL  +PP
Sbjct:    3 AAMMNKTIVVSKDGCARSSSIPKVATNKMGFASAVAMKKSRE--MMVWQPFNNKMFETFSYLPPLTDEQISKQVDYILANSWTPCLEFAASDQAYAGNENCIRMGPVASTYQDNRYWTMWKLPMFGCTDGSQVLSEIQACTKAFPDAYIRLVCFDANRQVQISGFLVHRPP 171          
BLAST of EY659414 vs. ExPASy Swiss-Prot
Match: RBS6_ACECL (Ribulose bisphosphate carboxylase small chain 6, chloroplastic OS=Acetabularia cliftonii GN=RBCS-6 PE=3 SV=1)

HSP 1 Score: 129.798 bits (325), Expect = 1.809e-29
Identity = 60/127 (47.24%), Postives = 81/127 (63.78%), Query Frame = 3
Query:  216 MKVWPPTGLKKFETLSYLPPLSDEALLKEISYLLRSGWIPCLEFEL-EKGWVYREHHSSPG-----YYDGRYWTMWKLPMYGCTDATQVLKEVGEVQKEYPHSFVRIIGFDNKRQVQCISFLAAKPP 578
            M VW P   K FET SYLPPL+DE + K++ Y+L + W PCLEF   ++ +   E+  + G     Y D RYWTMWKLPM+GCTD +QVL E+    K +P +++R++ FD  RQVQ   FL  +PP
Sbjct:   43 MMVWQPFNNKMFETFSYLPPLTDEQISKQVDYILANAWTPCLEFAASDQAYAGNENCITMGPVASTYQDNRYWTMWKLPMFGCTDGSQVLTEIQACTKAFPDAYIRLVCFDANRQVQISGFLVHRPP 169          
BLAST of EY659414 vs. ExPASy Swiss-Prot
Match: RBS3_ACEAT (Ribulose bisphosphate carboxylase small chain 3, chloroplastic OS=Acetabularia acetabulum GN=RBCS-3 PE=2 SV=1)

HSP 1 Score: 128.642 bits (322), Expect = 4.031e-29
Identity = 60/127 (47.24%), Postives = 81/127 (63.78%), Query Frame = 3
Query:  216 MKVWPPTGLKKFETLSYLPPLSDEALLKEISYLLRSGWIPCLEFEL-EKGWVYREHHSSPG-----YYDGRYWTMWKLPMYGCTDATQVLKEVGEVQKEYPHSFVRIIGFDNKRQVQCISFLAAKPP 578
            M VW P   K FET S+LPPL+DE + K++ Y+L + W PCLEF   ++ +   E+    G     Y D RYWTMWKLPM+GCTDA+QVL E+    K +P +++R++ FD  RQVQ   FL  +PP
Sbjct:   43 MMVWQPFNNKMFETFSFLPPLTDEQISKQVDYILINSWTPCLEFAASDQAYAGNENCIRMGPVASTYQDNRYWTMWKLPMFGCTDASQVLSEIQACTKAFPDAYIRLVCFDANRQVQISGFLVHRPP 169          
BLAST of EY659414 vs. ExPASy Swiss-Prot
Match: RBS_BATOE (Ribulose bisphosphate carboxylase small chain, chloroplastic OS=Batophora oerstedii GN=RBCS PE=2 SV=2)

HSP 1 Score: 127.872 bits (320), Expect = 6.876e-29
Identity = 61/136 (44.85%), Postives = 85/136 (62.50%), Query Frame = 3
Query:  192 SNG--GRVQCMKVWPPTGLKKFETLSYLPPLSDEALLKEISYLLRSGWIPCLEFE-LEKGWVYREHHSSPG-----YYDGRYWTMWKLPMYGCTDATQVLKEVGEVQKEYPHSFVRIIGFDNKRQVQCISFLAAKP 575
            SNG   + + M VW P   K FET SYLPPL+D+ + K++ Y+LR+ W PCLEF   ++ +V  ++    G     Y D RYWTMWKLPM+GC D +QVL E+    K +P +++R++ FD  RQVQ   FL  +P
Sbjct:   26 SNGTISKSRAMMVWEPFNNKFFETFSYLPPLTDDQITKQVDYILRNNWTPCLEFAGSDQAYVTHDNTVRMGDCASTYQDNRYWTMWKLPMFGCIDGSQVLTEISACTKAFPDAYIRLVCFDANRQVQISGFLVHRP 161          
The following BLAST results are available for this feature:
BLAST of EY659414 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 127
Match NameE-valueIdentityDescription
RBS2_CHLRE1.748e-3243.58Ribulose bisphosphate carboxylase small chain 2, c... [more]
RBS_SINAL2.284e-3277.92Ribulose bisphosphate carboxylase small chain (Fra... [more]
RBS_CHLMO2.284e-3247.20Ribulose bisphosphate carboxylase small chain, chl... [more]
RBS1_CHLRE2.982e-3243.02Ribulose bisphosphate carboxylase small chain 1, c... [more]
RBS3_ACECL5.625e-3144.74Ribulose bisphosphate carboxylase small chain 3, c... [more]
RBS4_ACECL4.761e-3042.68Ribulose bisphosphate carboxylase small chain 4, c... [more]
RBS5_ACECL6.219e-3040.35Ribulose bisphosphate carboxylase small chain 5, c... [more]
RBS6_ACECL1.809e-2947.24Ribulose bisphosphate carboxylase small chain 6, c... [more]
RBS3_ACEAT4.031e-2947.24Ribulose bisphosphate carboxylase small chain 3, c... [more]
RBS_BATOE6.876e-2944.85Ribulose bisphosphate carboxylase small chain, chl... [more]

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Properties
Property NameValue
Genbank descriptionCS00-C1-101-002-H04-CT.F Sweet orange leaf, infected with Xylella fastidiosa (stage 1 of 2) Citrus sinensis cDNA, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>EY659414 ID=EY659414; Name=EY659414; organism=Citrus sinensis; type=EST; length=822bp
CCACGCGTCCGCAAAGCTAGAAGAAGGAGCAAGTACCAATGGCTTCCTCA
ATGATCTCATCGACCACCGTTGCCACCGCGAACCGCGCCAGCCTTGCTCA
GGCCAGCATGGTCGCCCCCTTCACCGGCCTCAAGTCTTCTTCTGCTTTCC
CAGCAACCAAGAAGACCAACAATGACATTACTTCCATTGCAAGCAACGGC
GGAAGAGTCCAATGCATGAAGGTGTGGCCACCAACTGGCTTGAAGAAGTT
CGAGACTCTCTCATACCTCCCGCCACTGTCCGATGAAGCTTTGCTCAAGG
AAATCAGTTACCTTCTCCGCTCCGGCTGGATTCCCTGCTTGGAATTCGAG
TTGGAGAAAGGATGGGTGTACCGTGAGCACCACAGCTCACCAGGATACTA
CGATGGACGCTACTGGACAATGTGGAAGCTGCCCATGTATGGGTGCACAG
ATGCAACCCAGGTTTTGAAGGAGGTTGGGGAGGTCCAGAAGGAATACCCA
CATTCATTCGTCCGAATTATCGGATTTGACAACAAGCGTCAAGTGCAGTG
CATCAGTTTCCTTGCTGCCAAGCCACCAGGCGTCTAGGTTCCCAATTAAA
TTTGTGTAACCACCCATGCAATATTGTTGATTATTATTATGTGTAACCCC
CATAATGATCATTCGATCAATGGGGGTTCGGTTTGCGTTTAATTTGTACT
TANGCTGCTGTTGCTGCTGCTCAAACTTTTTTGGTTTTCTCCCCGACTTT
GGCTTTTTTTTTTATATATAATTTAAAAAATGAATCCTTNGTTTTCAGTT
TCATTAAAAAAAAAAAAAAAAA
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