EY659410

Overview
NameEY659410
Unique NameEY659410
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length888
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
BLAST of EY659410 vs. ExPASy Swiss-Prot
Match: ERF3_ZYGRO (Eukaryotic peptide chain release factor GTP-binding subunit OS=Zygosaccharomyces rouxii GN=SUP35 PE=3 SV=2)

HSP 1 Score: 205.682 bits (522), Expect = 2.927e-52
Identity = 102/246 (41.46%), Postives = 156/246 (63.41%), Query Frame = 2
Query:   26 ATTTGHLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVLDKLKAERERGITIDIALWKFETTKYYCTVIDAPGHRDFIKNMITGTSQADCAVLIIDSTTGGFEAGISKDGQTREHALLAFTLGVKQMICCCNKMDATTPKYSKARYDEIVKEVSSYLKKVGYN-PEKVPFVPISGFEGDNMIER--STNLDWYKGPTLLDALDNINEPKRPSDKPLRLPLQDVYKIGGIGTVPVGRVETGVLKPG 754
            +T  G+++Y  G +DKR +E++E+EA +  K+ +  +WV+D  + ER+ G TI++    FET K   T++DAPGH+ ++  MI G SQAD  +L+I +  G +E G  K GQTREHALLA T GV ++I   NKMD  T  +SK RYD+ VK +S++LK +GYN  E+V F+P+SG+ G  +  R       WY GP LL+ +DN++   R  + P  LP+    K+  +GT+  G++E+G ++ G
Sbjct:  251 STMGGNILYMTGSVDKRTVEKYEREAKDAGKQGWYLSWVMDTNREERDDGKTIEVGRAYFETEKRRYTILDAPGHKMYVSEMIGGASQADVGILVISARKGEYETGFEKGGQTREHALLAKTQGVNKLIVTINKMDDPTVNWSKERYDQCVKNLSNFLKAIGYNVKEEVVFMPVSGYSGAGLGTRVDPKECPWYDGPALLEYMDNMSHVDRKMNAPFMLPI--AAKMRDMGTIVEGKIESGHIRKG 494          
BLAST of EY659410 vs. ExPASy Swiss-Prot
Match: ERF3_KLULA (Eukaryotic peptide chain release factor GTP-binding subunit OS=Kluyveromyces lactis GN=SUP35 PE=3 SV=1)

HSP 1 Score: 204.142 bits (518), Expect = 8.516e-52
Identity = 107/246 (43.50%), Postives = 153/246 (62.20%), Query Frame = 2
Query:   26 ATTTGHLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVLDKLKAERERGITIDIALWKFETTKYYCTVIDAPGHRDFIKNMITGTSQADCAVLIIDSTTGGFEAGISKDGQTREHALLAFTLGVKQMICCCNKMDATTPKYSKARYDEIVKEVSSYLKKVGYN-PEKVPFVPISGFEGDNMIERSTNLD--WYKGPTLLDALDNINEPKRPSDKPLRLPLQDVYKIGGIGTVPVGRVETGVLKPG 754
            +T  G+L+Y  G +DKR +E++E+EA E  ++ +  +WV+D  K ER  G TI++    FET K   T++DAPGH+ ++  MI G SQAD  +L+I +  G +E G  K GQTREHALLA T GV +MI   NKMD  T  + K RYD  V  ++++LK VGYN  E V F+P+SG+ G  + ER    D  WY GP+LL+ LDN+    R  + P  LP+    K+  +GTV  G++E+G ++ G
Sbjct:  288 STMGGNLLYLTGSVDKRTVEKYEREAKEAGRQGWYLSWVMDTNKEERNDGKTIEVGRAYFETEKRRYTILDAPGHKMYVSEMIGGASQADIGILVISARKGEYETGFEKGGQTREHALLAKTQGVNKMIVVINKMDDPTVGWDKERYDHCVGNLTNFLKAVGYNVKEDVIFMPVSGYTGAGLKERVDPKDCPWYTGPSLLEYLDNMKTTDRHINAPFMLPIAS--KMKDMGTVVEGKIESGHIRKG 531          
BLAST of EY659410 vs. ExPASy Swiss-Prot
Match: ERF3_PICPI (Eukaryotic peptide chain release factor GTP-binding subunit OS=Pichia pinus GN=SUP2 PE=3 SV=1)

HSP 1 Score: 201.83 bits (512), Expect = 4.227e-51
Identity = 104/262 (39.69%), Postives = 161/262 (61.45%), Query Frame = 2
Query:   26 ATTTGHLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVLDKLKAERERGITIDIALWKFETTKYYCTVIDAPGHRDFIKNMITGTSQADCAVLIIDSTTGGFEAGISKDGQTREHALLAFTLGVKQMICCCNKMDATTPKYSKARYDEIVKEVSSYLKKVGYNPEKVPFVPISGFEGDNMIERSTNLD--WYKGPTLLDALDNINEPKRPSDKPLRLPLQDVYKIGGIGTVPVGRVETGVLKPGMVVTFGPSG*QLRXSLV 805
            +T  G+L++  G +DKR +E++E+EA +  ++ +  +W++D  K ER  G TI++    FET K   T++DAPGH+ +I  MI G SQAD  VL+I S  G +EAG  + GQ+REHA+LA T GV +++   NKMD  T  +SK RY+E   +++ YLK VGY    V F+P+SG+ G  + ER +  D  WY GP+LL+ LD++    R  + P  LP+    K+  +GTV  G++E+G +K G  +   P+  Q+  + +
Sbjct:  332 STMGGNLLFLTGAVDKRTVEKYEREAKDAGRQGWYLSWIMDTNKEERNDGKTIEVGKSYFETDKRRYTILDAPGHKLYISEMIGGASQADVGVLVISSRKGEYEAGFERGGQSREHAILAKTQGVNKLVVVINKMDDPTVNWSKERYEECTTKLAMYLKGVGYQKGDVLFMPVSGYTGAGLKERVSQKDAPWYNGPSLLEYLDSMPLAVRKINDPFMLPISS--KMKDLGTVIEGKIESGHVKKGQNLLVMPNKTQVEVTTI 591          
BLAST of EY659410 vs. ExPASy Swiss-Prot
Match: ERF3_CANMA (Eukaryotic peptide chain release factor GTP-binding subunit OS=Candida maltosa GN=SUP35 PE=3 SV=2)

HSP 1 Score: 199.904 bits (507), Expect = 1.606e-50
Identity = 100/253 (39.53%), Postives = 156/253 (61.66%), Query Frame = 2
Query:   26 ATTTGHLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVLDKLKAERERGITIDIALWKFETTKYYCTVIDAPGHRDFIKNMITGTSQADCAVLIIDSTTGGFEAGISKDGQTREHALLAFTLGVKQMICCCNKMDATTPKYSKARYDEIVKEVSSYLKKVGYNPEKVPFVPISGFEGDNMIERSTNLD--WYKGPTLLDALDNINEPKRPSDKPLRLPLQDVYKIGGIGTVPVGRVETGVLKPGMVVTFGPS 778
            +T  G+++Y  G +DKR +E++E+EA +  ++ +  +WV+D  K ER  G TI++    FET K   T++DAPGH+ ++  MI G SQAD  +L+I +  G +E G  K GQTREHALLA T GV ++I   NKMD  T  +SK RY E   ++  +LK +GYN + +  +P+SG+ G  + +R    D  WY+GP+LL+ LDN++   R  + P  +P+    K+  +GTV  G++E+G +K G  +   P+
Sbjct:  303 STMGGNILYLTGSVDKRTVEKYEREAKDAGRQGWYLSWVMDTNKEERNDGKTIEVGKAYFETDKRRYTILDAPGHKMYVSEMIGGASQADVGILVISARKGEYETGFEKGGQTREHALLAKTQGVNKIIVVVNKMDDPTVNWSKERYQECTTKLGVFLKGIGYNKDDIINMPVSGYTGAGLKDRVNPKDCPWYEGPSLLEYLDNMDTMNRKINGPFMMPVSG--KMKDLGTVVEGKIESGHVKKGTNLILMPN 553          
BLAST of EY659410 vs. ExPASy Swiss-Prot
Match: ERF3_CANAL (Eukaryotic peptide chain release factor GTP-binding subunit OS=Candida albicans GN=SUP35 PE=3 SV=1)

HSP 1 Score: 198.749 bits (504), Expect = 3.578e-50
Identity = 98/253 (38.74%), Postives = 157/253 (62.06%), Query Frame = 2
Query:   26 ATTTGHLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVLDKLKAERERGITIDIALWKFETTKYYCTVIDAPGHRDFIKNMITGTSQADCAVLIIDSTTGGFEAGISKDGQTREHALLAFTLGVKQMICCCNKMDATTPKYSKARYDEIVKEVSSYLKKVGYNPEKVPFVPISGFEGDNMIERSTNLD--WYKGPTLLDALDNINEPKRPSDKPLRLPLQDVYKIGGIGTVPVGRVETGVLKPGMVVTFGPS 778
            +T  G+++Y  G +DKR +E++E+EA +  ++ +  +WV+D  K ER  G TI++    FET K   T++DAPGH+ ++  MI G SQAD  +L+I +  G +E G  K GQTREHALLA T GV ++I   NKMD +T  +SK RY E   ++ ++LK +GY  + + ++P+SG+ G  + +R    D  WY GP+LL+ LDN++   R  + P  +P+    K+  +GT+  G++E+G +K G  +   P+
Sbjct:  306 STMGGNILYLTGSVDKRTVEKYEREAKDAGRQGWYLSWVMDTNKEERNDGKTIEVGKAYFETDKRRYTILDAPGHKMYVSEMIGGASQADVGILVISARKGEYETGFEKGGQTREHALLAKTQGVNKIIVVVNKMDDSTVGWSKERYQECTTKLGAFLKGIGYAKDDIIYMPVSGYTGAGLKDRVDPKDCPWYDGPSLLEYLDNMDTMNRKINGPFMMPVSG--KMKDLGTIVEGKIESGHVKKGTNLIMMPN 556          
BLAST of EY659410 vs. ExPASy Swiss-Prot
Match: EF1A_ENCCU (Elongation factor 1-alpha OS=Encephalitozoon cuniculi GN=TEF1 PE=1 SV=1)

HSP 1 Score: 197.208 bits (500), Expect = 1.041e-49
Identity = 101/262 (38.55%), Postives = 159/262 (60.69%), Query Frame = 2
Query:   26 ATTTGHLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVLDKLKAERERGITIDIALWKFETTKYYCTVIDAPGHRDFIKNMITGTSQADCAVLIIDSTTGGFEAGISKDGQTREHALLAFTLGVKQMICCCNKMDATTPKYSKARYDEIVKEVSSYLKKVGYNPEKVPFVPISGFEGDNMIERSTNLDWYKG----------PTLLDALDNINEPKRPSDKPLRLPLQDVYKIGGIGTVPVGRVETGVLKPGMVVTFGPSG 781
            +TT G L Y+LG +DKR +E++EKEAA  NK +F  A++ DK  AER+RGITI   L    T K+   ++D PGH+DF+KNM+TG SQAD AV+I+ ++  GFE+ +   G  + H +++  LG +++I C NKMD         +++E+  E+   +K+  +  +    +PIS F+G N+ ++    +W+KG           TL +AL+  + P+R +DKPLR+P+  V  I G+G +  GRVE G + P + +T  P+G
Sbjct:   60 STTVGMLSYQLGAVDKREMEKYEKEAALNNKETFYLAYLTDKTDAERKRGITITTTLVNLPTEKFNINILDCPGHKDFVKNMVTGASQADVAVVIVPAS--GFESCVGVGGMLKTHIMISGILGCEKLIVCVNKMDEIPENKRMEKFNEVSAEMLRIVKR-SHKDKNPIIIPISAFKGINLTKKGEKFEWFKGWKEKEGSSVIYTLEEALNYQDVPERHNDKPLRMPITKVCSIAGVGKIFTGRVEYGTITPNLKITIQPAG 318          
BLAST of EY659410 vs. ExPASy Swiss-Prot
Match: ERF3_DEBHA (Eukaryotic peptide chain release factor GTP-binding subunit OS=Debaryomyces hansenii GN=SUP35 PE=3 SV=4)

HSP 1 Score: 194.897 bits (494), Expect = 5.167e-49
Identity = 95/245 (38.78%), Postives = 154/245 (62.86%), Query Frame = 2
Query:   26 ATTTGHLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVLDKLKAERERGITIDIALWKFETTKYYCTVIDAPGHRDFIKNMITGTSQADCAVLIIDSTTGGFEAGISKDGQTREHALLAFTLGVKQMICCCNKMDATTPKYSKARYDEIVKEVSSYLKKVGYNPEKVPFVPISGFEGDNMIERSTNLD--WYKGPTLLDALDNINEPKRPSDKPLRLPLQDVYKIGGIGTVPVGRVETGVLKPG 754
            +T  G+++Y  G +DKR ++++E+EA +  ++ +  +WV+D  K ER  G TI++    FET K   T++DAPGH+ ++  MI G SQAD  +L+I +  G +E G  K GQTREHALLA T GV ++I   NKMD  T  +++ RY + + ++ ++LK +GY  + + F+P+SG+ G  + +R    D  WY GP+LL+ LDN+   +R  + P  LP+    K+  +GT+  G++E+G +K G
Sbjct:  292 STMGGNILYLTGSVDKRTVDKYEREAKDAGRQGWYLSWVMDTNKEERSDGKTIEVGKAYFETEKRRYTILDAPGHKMYVSEMIGGASQADVGILVISARKGEYETGFEKGGQTREHALLAKTQGVNKIIVVVNKMDDPTVGWAEDRYKDCITKLGTFLKGIGYAKDDIIFMPVSGYTGAGIKDRVNPKDCPWYSGPSLLEFLDNMKTMQRHINGPFMLPISG--KMKDMGTIIEGKIESGHIKKG 534          
BLAST of EY659410 vs. ExPASy Swiss-Prot
Match: ERF3_SCHPO (Eukaryotic peptide chain release factor GTP-binding subunit OS=Schizosaccharomyces pombe GN=sup35 PE=1 SV=2)

HSP 1 Score: 192.971 bits (489), Expect = 1.963e-48
Identity = 99/254 (38.98%), Postives = 158/254 (62.20%), Query Frame = 2
Query:   26 ATTTGHLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVLDKLKAERERGITIDIALWKFETTKYYCTVIDAPGHRDFIKNMITGTSQADCAVLIIDSTTGGFEAGISKDGQTREHALLAFTLGVKQMICCCNKMDATTPKYSKARYDEIVKEVSSYLKKV-GYNPE-KVPFVPISGFEGDNMIER--STNLDWYKGPTLLDALDNINEPKRPSDKPLRLPLQDVYKIGGIGTVPVGRVETGVLKPGMVVTFGP 775
            +T  G++++  G +DKR +E+ E+EA E  K S+  +W LD    ERE+G T+++    FET     +++DAPGH+ ++ NMI G SQAD  VL+I +  G FEAG  + GQTREHA+LA T G+  ++   NKMD  + ++S+ RY E V ++S +L++V GYN +  V ++P+S + G N+ +R  S+   WY+GP+LL+ LD++   +R  + P  +P+   YK   +GT+  G++E G +K    V   P
Sbjct:  252 STLGGNILFLTGMVDKRTMEKIEREAKEAGKESWYLSWALDSTSEEREKGKTVEVGRAYFETEHRRFSLLDAPGHKGYVTNMINGASQADIGVLVISARRGEFEAGFERGGQTREHAVLARTQGINHLVVVINKMDEPSVQWSEERYKECVDKLSMFLRRVAGYNSKTDVKYMPVSAYTGQNVKDRVDSSVCPWYQGPSLLEYLDSMTHLERKVNAPFIMPIASKYK--DLGTILEGKIEAGSIKKNSNVLVMP 503          
BLAST of EY659410 vs. ExPASy Swiss-Prot
Match: ERF3_DICDI (Eukaryotic peptide chain release factor GTP-binding subunit OS=Dictyostelium discoideum GN=erf3 PE=2 SV=1)

HSP 1 Score: 192.971 bits (489), Expect = 1.963e-48
Identity = 103/253 (40.71%), Postives = 158/253 (62.45%), Query Frame = 2
Query:   26 ATTTGHLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVLDKLKAERERGITIDIALWKFETTKYYCTVIDAPGHRDFIKNMITGTSQADCAVLIIDSTTGGFEAGISKDGQTREHALLAFTLGVKQMICCCNKMDATTPKYSKARYDEIVKEVSSYLKKVGYNPEK-VPFVPISGFEGDNMIERSTN--LDWYKGPTLLDALDNINEPKRPSDKPLRLPLQDVYKIGGIGTVPVGRVETGVLKPGMVVTFGP 775
            +T +G ++   G +D   + ++E+EA E ++  + YA+++D  + ER +G T+++    FETTK   T++DAPGHR ++ NMI G +QAD  +L+I S  G FEAG+ + GQT EHA LA  +G+K ++   NKMD  T K+SKARYDEI  +++ +LKK G+NP+K   FVP SG+   N++        DWY GP+L+  LDN++  +R     LR+P+   YK  GI  V +G+VE+G +  G  +   P
Sbjct:  131 STLSGSIMVLTGQVDPHTLAKYEREAKENHREGWIYAYIMDTNEEERTKGKTVEVGRAHFETTKKRYTILDAPGHRLYVPNMIIGAAQADVGILVISSKKGEFEAGV-EGGQTIEHARLAKMIGIKYLVVFVNKMDEPTVKWSKARYDEITDKLTVHLKKCGWNPKKDFHFVPGSGYGTLNVLAPLAPGVCDWYSGPSLIGTLDNLSGMERNEGGALRIPITTSYKDRGIVNV-IGKVESGTISVGQSIHIMP 381          
BLAST of EY659410 vs. ExPASy Swiss-Prot
Match: HBS1_YEAST (Elongation factor 1 alpha-like protein OS=Saccharomyces cerevisiae GN=HBS1 PE=1 SV=2)

HSP 1 Score: 181.03 bits (458), Expect = 7.721e-45
Identity = 99/263 (37.64%), Postives = 153/263 (58.17%), Query Frame = 2
Query:   26 ATTTGHLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVLDKLKAERERGITIDIALWKFETTKYYCTVIDAPGHRDFIKNMITGTSQADCAVLIIDSTTGGFEAGISKDGQTREHALLAFTLGVKQMICCCNKMDATTPKYSKARYDEIVKEVSSYLKKVGYNPEKVPFVPISGFEGDNM--IERSTNL-DWYKGPTLLDALDNI-------NEPKRPSDKPLRLPLQDV--YKIGGIGTVPVGRVETGVLKPGMVVTFGPS 778
            +T  G L+Y L  +++  + + ++E+  M K SFK+AW++D+   ERERG+T+ I    F T +   T++DAPGHRDF+ N I G SQAD A+L +D +T  FE+G   DGQT+EH LLA +LG+  +I   NKMD     +S+ R++EI  ++  YL  +G+  + + +VPISGF G+ +  IE +  +  WY GP L+  L+N        NE     D  L   L+ +   K      +  G++E+G ++PG  +T  PS
Sbjct:  181 STLMGRLLYDLNIVNQSQLRKLQRESETMGKSSFKFAWIMDQTNEERERGVTVSICTSHFSTHRANFTIVDAPGHRDFVPNAIMGISQADMAILCVDCSTNAFESGFDLDGQTKEHMLLASSLGIHNLIIAMNKMD--NVDWSQQRFEEIKSKLLPYLVDIGFFEDNINWVPISGFSGEGVYKIEYTDEVRQWYNGPNLMSTLENAAFKISKENEGINKDDPFLFSVLEIIPSKKTSNDLALVSGKLESGSIQPGESLTIYPS 441          
The following BLAST results are available for this feature:
BLAST of EY659410 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 500
Match NameE-valueIdentityDescription
ERF3_ZYGRO2.927e-5241.46Eukaryotic peptide chain release factor GTP-bindin... [more]
ERF3_KLULA8.516e-5243.50Eukaryotic peptide chain release factor GTP-bindin... [more]
ERF3_PICPI4.227e-5139.69Eukaryotic peptide chain release factor GTP-bindin... [more]
ERF3_CANMA1.606e-5039.53Eukaryotic peptide chain release factor GTP-bindin... [more]
ERF3_CANAL3.578e-5038.74Eukaryotic peptide chain release factor GTP-bindin... [more]
EF1A_ENCCU1.041e-4938.55Elongation factor 1-alpha OS=Encephalitozoon cunic... [more]
ERF3_DEBHA5.167e-4938.78Eukaryotic peptide chain release factor GTP-bindin... [more]
ERF3_SCHPO1.963e-4838.98Eukaryotic peptide chain release factor GTP-bindin... [more]
ERF3_DICDI1.963e-4840.71Eukaryotic peptide chain release factor GTP-bindin... [more]
HBS1_YEAST7.721e-4537.64Elongation factor 1 alpha-like protein OS=Saccharo... [more]

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Properties
Property NameValue
Genbank descriptionCS00-C1-101-002-G12-CT.F Sweet orange leaf, infected with Xylella fastidiosa (stage 1 of 2) Citrus sinensis cDNA, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>EY659410 ID=EY659410; Name=EY659410; organism=Citrus sinensis; type=EST; length=888bp
CCACGCGTCCGCGACTCTGGTAAACGCAACCACCACCGGACACTTGATCT
ACAAGCTTGGTGGTATTGACAAGCGTGTGATCGAGAGGTTCGAGAAGGAA
GCTGCTGAGATGAACAAGAGGTCATTCAAGTATGCCTGGGTGCTCGACAA
GCTCAAGGCTGAGCGTGAACGTGGTATTACCATTGATATTGCCTTGTGGA
AGTTCGAGACCACCAAGTACTACTGCACCGTCATTGATGCTCCTGGACAT
CGTGACTTTATCAAGAACATGATTACCGGTACCTCACAGGCTGACTGTGC
CGTCCTTATCATTGACTCCACCACCGGTGGTTTTGAAGCTGGTATCTCCA
AGGATGGTCAGACTCGTGAGCATGCTTTGCTTGCTTTCACCCTTGGTGTG
AAGCAGATGATTTGCTGCTGTAACAAGATGGACGCCACCACCCCCAAGTA
CTCAAAGGCTAGGTACGATGAAATTGTGAAGGAGGTCTCTTCCTACCTGA
AGAAGGTTGGATACAACCCCGAGAAAGTCCCATTCGTGCCCATCTCTGGT
TTCGAGGGTGACAACATGATTGAGAGGTCTACCAACCTTGACTGGTACAA
GGGTCCAACCCTGCTGGATGCTCTTGACAATATCAATGAGCCGAAGAGGC
CCTCAGACAAGCCCCTTCGTCTGCCACTCCAGGACGTGTACAAGATTGGT
GGCATTGGAACCGTCCCAGTGGGTCGCGTTGAGACCGGTGTCCTCAAGCC
TGGTATGGTGGTGACATTTGGCCCCTCTGGATGACAACTGAGGNTAAGTC
TNGTGAGATGCACACGAAGCTCTGCAGGAGGCCCTTCGGGTGACATGNTG
GCTTCACGTGAAGAACGTGCTGTCAAGATCTCAGCGTG
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