EY705313

Overview
NameEY705313
Unique NameEY705313
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length922
Libraries
Library NameType
Sweet orange fruit, development stadium (2 of 6)cdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of EY705313 vs. ExPASy Swiss-Prot
Match: NDKA_FLABI (Nucleoside diphosphate kinase A OS=Flaveria bidentis PE=2 SV=1)

HSP 1 Score: 97.8265 bits (242), Expect = 2.758e-30
Identity = 45/78 (57.69%), Postives = 58/78 (74.36%), Query Frame = 1 
Query:  412 MERTFIAIKPDGVQRGLISEIISRFERKGFKLVAIKIVVPSKEFAQKHYHDLKERPFFNGLCEFLSSGP--SLPWYGK 639
            ME+TFI IKPDGVQ GL+ EII RFE+KGF L  +K++   + FA+KHY DL  +PFFNGL E++ SGP  ++ W GK
Sbjct:    1 MEQTFIMIKPDGVQSGLVGEIIGRFEKKGFSLKGLKLLTVDRAFAEKHYADLSSKPFFNGLVEYIVSGPVVAMVWEGK 78

HSP 2 Score: 55.8398 bits (133), Expect = 2.758e-30
Identity = 26/47 (55.32%), Postives = 34/47 (72.34%), Query Frame = 3 
Query:  618 VIAMVWEGEXSITYGRKLIGATDPQKSEPGNIRGDLAGVV*RNSFMG 758
            V+AMVWEG+  +T GR++IGAT+P +S PG IRGD A  + RN   G
Sbjct:   70 VVAMVWEGKNVVTTGREIIGATNPAESAPGTIRGDFAIDIGRNVIHG 116
[Close]
BLAST of EY705313 vs. ExPASy Swiss-Prot
Match: NDK1_PEA (Nucleoside diphosphate kinase 1 OS=Pisum sativum GN=NDPK1 PE=2 SV=1)

HSP 1 Score: 94.3597 bits (233), Expect = 2.758e-30
Identity = 45/77 (58.44%), Postives = 58/77 (75.32%), Query Frame = 1 
Query:  415 ERTFIAIKPDGVQRGLISEIISRFERKGFKLVAIKIVVPSKEFAQKHYHDLKERPFFNGLCEFLSSGP--SLPWYGK 639
            E+TFI IKPDGVQRGL+ EIISRFE+KGF L  +K V   + FA+KHY DL  +PFF+GL +++ SGP  ++ W GK
Sbjct:    3 EQTFIMIKPDGVQRGLVGEIISRFEKKGFYLKGLKFVNVERAFAEKHYADLSAKPFFSGLVDYIISGPVVAMIWEGK 79

HSP 2 Score: 59.3066 bits (142), Expect = 2.758e-30
Identity = 27/47 (57.45%), Postives = 35/47 (74.47%), Query Frame = 3 
Query:  618 VIAMVWEGEXSITYGRKLIGATDPQKSEPGNIRGDLAGVV*RNSFMG 758
            V+AM+WEG+  +T GRK+IGAT+P +SEPG IRGD A  + RN   G
Sbjct:   71 VVAMIWEGKNVVTTGRKIIGATNPAQSEPGTIRGDFAIDIGRNVIHG 117
[Close]
BLAST of EY705313 vs. ExPASy Swiss-Prot
Match: NDK_CYAP4 (Nucleoside diphosphate kinase OS=Cyanothece sp. (strain PCC 7425 / ATCC 29141) GN=ndk PE=3 SV=1)

HSP 1 Score: 99.3673 bits (246), Expect = 6.090e-30
Identity = 46/78 (58.97%), Postives = 62/78 (79.49%), Query Frame = 1 
Query:  412 MERTFIAIKPDGVQRGLISEIISRFERKGFKLVAIKIVVPSKEFAQKHYHDLKERPFFNGLCEFLSSGP--SLPWYGK 639
            MERTF+A+KPDGVQR L+ EII RFE KGFKLV +K++  SK+ A++HY + KE+PFF GL +F++SGP  ++ W GK
Sbjct:    1 MERTFLAVKPDGVQRALVGEIIRRFEAKGFKLVGLKLMNVSKDLAEQHYGEHKEKPFFPGLVQFITSGPVVAMVWEGK 78

HSP 2 Score: 53.1434 bits (126), Expect = 6.090e-30
Identity = 25/47 (53.19%), Postives = 31/47 (65.96%), Query Frame = 3 
Query:  618 VIAMVWEGEXSITYGRKLIGATDPQKSEPGNIRGDLAGVV*RNSFMG 758
            V+AMVWEG+  +   RK+IGAT+P  SEPG IRGD    + RN   G
Sbjct:   70 VVAMVWEGKGVVASARKIIGATNPLNSEPGTIRGDYGVDIGRNIIHG 116
[Close]
BLAST of EY705313 vs. ExPASy Swiss-Prot
Match: NDKB_PONAB (Nucleoside diphosphate kinase B OS=Pongo abelii GN=NME2 PE=2 SV=1)

HSP 1 Score: 100.138 bits (248), Expect = 1.343e-29
Identity = 47/77 (61.04%), Postives = 63/77 (81.82%), Query Frame = 1 
Query:  412 MERTFIAIKPDGVQRGLISEIISRFERKGFKLVAIKIVVPSKEFAQKHYHDLKERPFFNGLCEFLSSGP--SLPWYG 636
            +ERTFIAIKPDGVQRGL+ EII RFE+KGF+LVA+K +  S+E  ++HY DLK+RPFF GL ++++SGP  ++ W G
Sbjct:    4 LERTFIAIKPDGVQRGLVGEIIKRFEQKGFRLVAMKFLRASEEHLKQHYTDLKDRPFFPGLVKYMNSGPVVAMVWEG 80

HSP 2 Score: 51.2174 bits (121), Expect = 1.343e-29
Identity = 32/80 (40.00%), Postives = 43/80 (53.75%), Query Frame = 3 
Query:  618 VIAMVWEGEXSITYGRKLIGATDPQKSEPGNIRGDLAGVV*RNSFMGV-MARDRKDEINCGSNPKTG*LHKH-AEKWVYE 851
            V+AMVWEG   +  GR ++G T+P  S+PG IRGD    V RN   G    +  + EI+    P+    +K  A  WVYE
Sbjct:   73 VVAMVWEGLNVVKTGRLMLGETNPADSKPGTIRGDFCIQVGRNIIHGSDSVKSAEKEISLWFKPEELVDYKSCAHDWVYE 152
[Close]
BLAST of EY705313 vs. ExPASy Swiss-Prot
Match: NDKB_BOVIN (Nucleoside diphosphate kinase B OS=Bos taurus GN=NME2 PE=2 SV=1)

HSP 1 Score: 99.7525 bits (247), Expect = 1.343e-29
Identity = 47/76 (61.84%), Postives = 62/76 (81.58%), Query Frame = 1 
Query:  415 ERTFIAIKPDGVQRGLISEIISRFERKGFKLVAIKIVVPSKEFAQKHYHDLKERPFFNGLCEFLSSGP--SLPWYG 636
            ERTFIAIKPDGVQRGL+ EII RFE+KGF+LVA+K +  S+E  ++HY DLK+RPFF GL ++++SGP  ++ W G
Sbjct:    5 ERTFIAIKPDGVQRGLVGEIIKRFEQKGFRLVAMKFLQASEELLKQHYIDLKDRPFFPGLVKYMNSGPVVAMVWEG 80

HSP 2 Score: 51.6026 bits (122), Expect = 1.343e-29
Identity = 32/80 (40.00%), Postives = 43/80 (53.75%), Query Frame = 3 
Query:  618 VIAMVWEGEXSITYGRKLIGATDPQKSEPGNIRGDLAGVV*RNSFMGV-MARDRKDEINCGSNPKTG*LHKH-AEKWVYE 851
            V+AMVWEG   +  GR ++G T+P  S+PG IRGD    V RN   G    +  + EIN    P+    +K  A  W+YE
Sbjct:   73 VVAMVWEGLNVVKTGRVMLGETNPADSKPGTIRGDFCIQVGRNIIHGSDSVKSAEKEINLWFKPEELIEYKPCAFDWIYE 152
[Close]
BLAST of EY705313 vs. ExPASy Swiss-Prot
Match: NDKB_HUMAN (Nucleoside diphosphate kinase B OS=Homo sapiens GN=NME2 PE=1 SV=1)

HSP 1 Score: 99.7525 bits (247), Expect = 1.749e-29
Identity = 47/77 (61.04%), Postives = 63/77 (81.82%), Query Frame = 1 
Query:  412 MERTFIAIKPDGVQRGLISEIISRFERKGFKLVAIKIVVPSKEFAQKHYHDLKERPFFNGLCEFLSSGP--SLPWYG 636
            +ERTFIAIKPDGVQRGL+ EII RFE+KGF+LVA+K +  S+E  ++HY DLK+RPFF GL ++++SGP  ++ W G
Sbjct:    4 LERTFIAIKPDGVQRGLVGEIIKRFEQKGFRLVAMKFLRASEEHLKQHYIDLKDRPFFPGLVKYMNSGPVVAMVWEG 80

HSP 2 Score: 51.2174 bits (121), Expect = 1.749e-29
Identity = 32/80 (40.00%), Postives = 43/80 (53.75%), Query Frame = 3 
Query:  618 VIAMVWEGEXSITYGRKLIGATDPQKSEPGNIRGDLAGVV*RNSFMGV-MARDRKDEINCGSNPKTG*LHKH-AEKWVYE 851
            V+AMVWEG   +  GR ++G T+P  S+PG IRGD    V RN   G    +  + EI+    P+    +K  A  WVYE
Sbjct:   73 VVAMVWEGLNVVKTGRVMLGETNPADSKPGTIRGDFCIQVGRNIIHGSDSVKSAEKEISLWFKPEELVDYKSCAHDWVYE 152
[Close]
BLAST of EY705313 vs. ExPASy Swiss-Prot
Match: NDK_CYAA5 (Nucleoside diphosphate kinase OS=Cyanothece sp. (strain ATCC 51142) GN=ndk PE=3 SV=1)

HSP 1 Score: 94.3597 bits (233), Expect = 2.280e-29
Identity = 46/77 (59.74%), Postives = 56/77 (72.73%), Query Frame = 1 
Query:  412 MERTFIAIKPDGVQRGLISEIISRFERKGFKLVAIKIVVPSKEFAQKHYHDLKERPFFNGLCEFLSSGP--SLPWYG 636
            MERTFI IKPDGVQRGL+ E+I RFE KGF LV +K++  SKE A++HY   KERPFF  L EF+ S P  ++ W G
Sbjct:    1 MERTFIMIKPDGVQRGLVGEVIGRFEAKGFTLVGLKLMSVSKELAEEHYDVHKERPFFGSLVEFICSSPVVAMVWEG 77

HSP 2 Score: 56.225 bits (134), Expect = 2.280e-29
Identity = 35/80 (43.75%), Postives = 44/80 (55.00%), Query Frame = 3 
Query:  618 VIAMVWEGEXSITYGRKLIGATDPQKSEPGNIRGDLAGVV*RNSFMGVMA-RDRKDEINCGSNPK-TG*LHKHAEKWVYE 851
            V+AMVWEG+  +   RKLIGAT+P  +EPG IRGD    V RN   G  A    + EI+   N K        A+ W+YE
Sbjct:   70 VVAMVWEGDGVVASARKLIGATNPLSAEPGTIRGDFGVSVGRNLIHGSDAIETAQREISLWFNEKELSSWEPTAKTWLYE 149
[Close]
BLAST of EY705313 vs. ExPASy Swiss-Prot
Match: NDKB_MOUSE (Nucleoside diphosphate kinase B OS=Mus musculus GN=Nme2 PE=1 SV=1)

HSP 1 Score: 99.7525 bits (247), Expect = 2.966e-29
Identity = 47/77 (61.04%), Postives = 63/77 (81.82%), Query Frame = 1 
Query:  412 MERTFIAIKPDGVQRGLISEIISRFERKGFKLVAIKIVVPSKEFAQKHYHDLKERPFFNGLCEFLSSGP--SLPWYG 636
            +ERTFIAIKPDGVQRGL+ EII RFE+KGF+LVA+K +  S+E  ++HY DLK+RPFF GL ++++SGP  ++ W G
Sbjct:    4 LERTFIAIKPDGVQRGLVGEIIKRFEQKGFRLVAMKFLRASEEHLKQHYIDLKDRPFFPGLVKYMNSGPVVAMVWEG 80

HSP 2 Score: 50.447 bits (119), Expect = 2.966e-29
Identity = 32/80 (40.00%), Postives = 42/80 (52.50%), Query Frame = 3 
Query:  618 VIAMVWEGEXSITYGRKLIGATDPQKSEPGNIRGDLAGVV*RNSFMGV-MARDRKDEINCGSNPKTG*LHKH-AEKWVYE 851
            V+AMVWEG   +  GR ++G T+P  S+PG IRGD    V RN   G       + EI+    P+    +K  A  WVYE
Sbjct:   73 VVAMVWEGLNVVKTGRVMLGETNPADSKPGTIRGDFCIQVGRNIIHGSDSVESAEKEIHLWFKPEELIDYKSCAHDWVYE 152
[Close]
BLAST of EY705313 vs. ExPASy Swiss-Prot
Match: NDKA_MOUSE (Nucleoside diphosphate kinase A OS=Mus musculus GN=Nme1 PE=1 SV=1)

HSP 1 Score: 97.8265 bits (242), Expect = 2.966e-29
Identity = 45/76 (59.21%), Postives = 60/76 (78.95%), Query Frame = 1 
Query:  415 ERTFIAIKPDGVQRGLISEIISRFERKGFKLVAIKIVVPSKEFAQKHYHDLKERPFFNGLCEFLSSGP--SLPWYG 636
            ERTFIAIKPDGVQRGL+ EII RFE+KGF+LV +K +  S++  ++HY DLK+RPFF GL +++ SGP  ++ W G
Sbjct:    5 ERTFIAIKPDGVQRGLVGEIIKRFEQKGFRLVGLKFLQASEDLLKEHYTDLKDRPFFTGLVKYMHSGPVVAMVWEG 80

HSP 2 Score: 52.373 bits (124), Expect = 2.966e-29
Identity = 31/80 (38.75%), Postives = 44/80 (55.00%), Query Frame = 3 
Query:  618 VIAMVWEGEXSITYGRKLIGATDPQKSEPGNIRGDLAGVV*RNSFMGV-MARDRKDEINCGSNPKTG*LHKH-AEKWVYE 851
            V+AMVWEG   +  GR ++G T+P  S+PG IRGD    V RN   G    +  + EI+    P+    +K  A+ W+YE
Sbjct:   73 VVAMVWEGLNVVKTGRVMLGETNPADSKPGTIRGDFCIQVGRNIIHGSDSVKSAEKEISLWFQPEELVEYKSCAQNWIYE 152
[Close]
BLAST of EY705313 vs. ExPASy Swiss-Prot
Match: NDK_SYNP6 (Nucleoside diphosphate kinase OS=Synechococcus sp. (strain ATCC 27144 / PCC 6301 / SAUG 1402/1) GN=ndk PE=3 SV=2)

HSP 1 Score: 102.834 bits (255), Expect = 2.969e-29
Identity = 48/69 (69.57%), Postives = 58/69 (84.06%), Query Frame = 1 
Query:  412 MERTFIAIKPDGVQRGLISEIISRFERKGFKLVAIKIVVPSKEFAQKHYHDLKERPFFNGLCEFLSSGP 618
            MERTFIAIKPDGVQRGL+  II RFE+KGFKLV +K + PS+E A++HY   +ERPFFNGL EF++SGP
Sbjct:    1 MERTFIAIKPDGVQRGLVGTIIGRFEQKGFKLVGLKQLKPSRELAEQHYAVHRERPFFNGLVEFITSGP 69

HSP 2 Score: 47.3654 bits (111), Expect = 2.969e-29
Identity = 23/47 (48.94%), Postives = 30/47 (63.83%), Query Frame = 3 
Query:  618 VIAMVWEGEXSITYGRKLIGATDPQKSEPGNIRGDLAGVV*RNSFMG 758
            ++A+V EGE  +   RKLIGAT+P  +EPG IRGD    + RN   G
Sbjct:   70 IVAIVLEGEGVVAAARKLIGATNPLTAEPGTIRGDFGVNIGRNIIHG 116
The following BLAST results are available for this feature:
BLAST of EY705313 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 500
Match NameE-valueIdentityDescription
NDKA_FLABI2.758e-3057.69Nucleoside diphosphate kinase A OS=Flaveria bident... [more]
NDK1_PEA2.758e-3058.44Nucleoside diphosphate kinase 1 OS=Pisum sativum G... [more]
NDK_CYAP46.090e-3058.97Nucleoside diphosphate kinase OS=Cyanothece sp. (s... [more]
NDKB_PONAB1.343e-2961.04Nucleoside diphosphate kinase B OS=Pongo abelii GN... [more]
NDKB_BOVIN1.343e-2961.84Nucleoside diphosphate kinase B OS=Bos taurus GN=N... [more]
NDKB_HUMAN1.749e-2961.04Nucleoside diphosphate kinase B OS=Homo sapiens GN... [more]
NDK_CYAA52.280e-2959.74Nucleoside diphosphate kinase OS=Cyanothece sp. (s... [more]
NDKB_MOUSE2.966e-2961.04Nucleoside diphosphate kinase B OS=Mus musculus GN... [more]
NDKA_MOUSE2.966e-2959.21Nucleoside diphosphate kinase A OS=Mus musculus GN... [more]
NDK_SYNP62.969e-2969.57Nucleoside diphosphate kinase OS=Synechococcus sp.... [more]

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Properties
Property NameValue
Genbank descriptionCS00-C3-701-042-B10-CT.F Sweet orange fruit, development stadium (2 of 6) Citrus sinensis cDNA, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>EY705313 ID=EY705313; Name=EY705313; organism=Citrus sinensis; type=EST; length=922bp
TGCAGACCCACGCGTCCGGTTTTCTGATTATCTTTATGTCTGTTTTGCAA
GTTGTTACTGACTGCACTTCATATTATGAAAATCTGCAAGCCAAAGTTTC
TTATCAATCTGATAAAACCTTCACTCTCTTTACTAAAGGTGGATCTAAAA
GAAGAAATTAATATTGTTGATAAAAGCTTGGATAACCATTTCTTCACAAT
TTTATCTCTTCAAGAGAACAATAATAGGAAATATTTTATGTAACTTTATG
ACATCATAGCTTTGAATATTAATTGCACGAAGTTGCGCCTTCCTTTTTCC
TCCAAAATCTCTGATCTTAATTTTTCCTTTGTGCTTTTCTTTATTATTGC
AGCTTACATGCTCCAGGAGCAGGAAGTACATGCTGCAGAGGTACTTTAAT
CACTGATCTAAATGGAACGCACTTTCATTGCCATCAAGCCAGATGGGGTG
CAAAGAGGACTGATTTCAGAAATCATCTCTCGCTTTGAGCGCAAAGGCTT
TAAGCTTGTGGCCATTAAGATAGTAGTTCCATCAAAAGAGTTTGCACAAA
AACATTATCATGATCTGAAGGAAAGACCCTTCTTTAATGGCCTGTGCGAG
TTCCTTAGCTCTGGTCCGTCATTGCCATGGTATGGGAAGGAGAGGNNAGT
ATTACGTATGGTCGCAAACTCATTGGAGCCACAGATCCTCAGAAATCAGA
ACCTGGAAACATCAGAGGCGATCTAGCTGGTGTTGTTTGAAGAAACTCAT
TCATGGGAGTGATGGCCCGAGACCGCAAGGATGAAATAAATTGTGGGTCC
AACCCCAAGACTGGTTAATTACACAAGCATGCAGAGAAGTGGGTCTATGA
ATCCACTGATTACATTTTTCTCTACTTTATTAAAACTAAGGCCTACTGAA
TAGGGGATAAAAGCATAATGGT
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