EY705313

Overview
NameEY705313
Unique NameEY705313
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length922
Libraries
Library NameType
Sweet orange fruit, development stadium (2 of 6)cdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of EY705313 vs. ExPASy Swiss-Prot
Match: NDK_SYNE7 (Nucleoside diphosphate kinase OS=Synechococcus elongatus (strain PCC 7942) GN=ndk PE=3 SV=2)

HSP 1 Score: 102.834 bits (255), Expect = 2.969e-29
Identity = 48/69 (69.57%), Postives = 58/69 (84.06%), Query Frame = 1 
Query:  412 MERTFIAIKPDGVQRGLISEIISRFERKGFKLVAIKIVVPSKEFAQKHYHDLKERPFFNGLCEFLSSGP 618
            MERTFIAIKPDGVQRGL+  II RFE+KGFKLV +K + PS+E A++HY   +ERPFFNGL EF++SGP
Sbjct:    1 MERTFIAIKPDGVQRGLVGTIIGRFEQKGFKLVGLKQLKPSRELAEQHYAVHRERPFFNGLVEFITSGP 69

HSP 2 Score: 47.3654 bits (111), Expect = 2.969e-29
Identity = 23/47 (48.94%), Postives = 30/47 (63.83%), Query Frame = 3 
Query:  618 VIAMVWEGEXSITYGRKLIGATDPQKSEPGNIRGDLAGVV*RNSFMG 758
            ++A+V EGE  +   RKLIGAT+P  +EPG IRGD    + RN   G
Sbjct:   70 IVAIVLEGEGVVAAARKLIGATNPLTAEPGTIRGDFGVNIGRNIIHG 116
[Close]
BLAST of EY705313 vs. ExPASy Swiss-Prot
Match: NDK1_ARATH (Nucleoside diphosphate kinase 1 OS=Arabidopsis thaliana GN=NDPK1 PE=1 SV=1)

HSP 1 Score: 93.9745 bits (232), Expect = 2.969e-29
Identity = 43/78 (55.13%), Postives = 58/78 (74.36%), Query Frame = 1 
Query:  412 MERTFIAIKPDGVQRGLISEIISRFERKGFKLVAIKIVVPSKEFAQKHYHDLKERPFFNGLCEFLSSGP--SLPWYGK 639
            ME+TFI IKPDGVQRGLI E+I RFE+KGF L  +K++   + FA+KHY DL  + FF+GL +++ SGP  ++ W GK
Sbjct:    1 MEQTFIMIKPDGVQRGLIGEVICRFEKKGFTLKGLKLISVERSFAEKHYEDLSSKSFFSGLVDYIVSGPVVAMIWEGK 78

HSP 2 Score: 56.225 bits (134), Expect = 2.969e-29
Identity = 26/47 (55.32%), Postives = 33/47 (70.21%), Query Frame = 3 
Query:  618 VIAMVWEGEXSITYGRKLIGATDPQKSEPGNIRGDLAGVV*RNSFMG 758
            V+AM+WEG+  +  GRK+IGAT+P  SEPG IRGD A  + RN   G
Sbjct:   70 VVAMIWEGKNVVLTGRKIIGATNPAASEPGTIRGDFAIDIGRNVIHG 116
[Close]
BLAST of EY705313 vs. ExPASy Swiss-Prot
Match: NDK_SYNP2 (Nucleoside diphosphate kinase OS=Synechococcus sp. (strain ATCC 27264 / PCC 7002 / PR-6) GN=ndk PE=3 SV=1)

HSP 1 Score: 93.2041 bits (230), Expect = 3.439e-29
Identity = 44/78 (56.41%), Postives = 58/78 (74.36%), Query Frame = 1 
Query:  412 MERTFIAIKPDGVQRGLISEIISRFERKGFKLVAIKIVVPSKEFAQKHYHDLKERPFFNGLCEFLSSGP--SLPWYGK 639
            MERTF+ +KPDGVQRGL+ ++I RFE KGFKLV +K+V  S+E A++HY   +ERPFF  L EF+ S P  ++ W GK
Sbjct:    1 MERTFVMVKPDGVQRGLVGDVIRRFEAKGFKLVGLKLVSVSRELAEQHYGVHRERPFFGSLVEFIISVPVVAMVWEGK 78

HSP 2 Score: 54.299 bits (129), Expect = 3.439e-29
Identity = 25/47 (53.19%), Postives = 33/47 (70.21%), Query Frame = 3 
Query:  618 VIAMVWEGEXSITYGRKLIGATDPQKSEPGNIRGDLAGVV*RNSFMG 758
            V+AMVWEG+ +I   RK+IGAT+P ++EPG IRGD    + RN   G
Sbjct:   70 VVAMVWEGKGAIAAARKIIGATNPLEAEPGTIRGDFGVDIGRNLIHG 116

HSP 3 Score: 22.7126 bits (47), Expect = 3.439e-29
Identity = 8/10 (80.00%), Postives = 9/10 (90.00%), Query Frame = 2 
Query:  740 KKLIHGSDGP 769
            + LIHGSDGP
Sbjct:  111 RNLIHGSDGP 120
[Close]
BLAST of EY705313 vs. ExPASy Swiss-Prot
Match: NDKB_RAT (Nucleoside diphosphate kinase B OS=Rattus norvegicus GN=Nme2 PE=1 SV=1)

HSP 1 Score: 99.7525 bits (247), Expect = 3.861e-29
Identity = 47/77 (61.04%), Postives = 63/77 (81.82%), Query Frame = 1 
Query:  412 MERTFIAIKPDGVQRGLISEIISRFERKGFKLVAIKIVVPSKEFAQKHYHDLKERPFFNGLCEFLSSGP--SLPWYG 636
            +ERTFIAIKPDGVQRGL+ EII RFE+KGF+LVA+K +  S+E  ++HY DLK+RPFF GL ++++SGP  ++ W G
Sbjct:    4 LERTFIAIKPDGVQRGLVGEIIKRFEQKGFRLVAMKFLRASEEHLKQHYIDLKDRPFFPGLVKYMNSGPVVAMVWEG 80

HSP 2 Score: 50.0618 bits (118), Expect = 3.861e-29
Identity = 32/80 (40.00%), Postives = 41/80 (51.25%), Query Frame = 3 
Query:  618 VIAMVWEGEXSITYGRKLIGATDPQKSEPGNIRGDLAGVV*RNSFMGV-MARDRKDEINCGSNPKTG*LHKH-AEKWVYE 851
            V+AMVWEG   +  GR ++G T+P  S+PG IRGD    V RN   G       + EI     P+    +K  A  WVYE
Sbjct:   73 VVAMVWEGLNVVKTGRVMLGETNPADSKPGTIRGDFCIQVGRNIIHGSDSVESAEKEIGLWFKPEELIDYKSCAHDWVYE 152
[Close]
BLAST of EY705313 vs. ExPASy Swiss-Prot
Match: NDKA_RAT (Nucleoside diphosphate kinase A OS=Rattus norvegicus GN=Nme1 PE=1 SV=1)

HSP 1 Score: 98.5969 bits (244), Expect = 3.861e-29
Identity = 45/76 (59.21%), Postives = 61/76 (80.26%), Query Frame = 1 
Query:  415 ERTFIAIKPDGVQRGLISEIISRFERKGFKLVAIKIVVPSKEFAQKHYHDLKERPFFNGLCEFLSSGP--SLPWYG 636
            ERTFIAIKPDGVQRGL+ EII RFE+KGF+LV +K +  S++  ++HY DLK+RPFF+GL +++ SGP  ++ W G
Sbjct:    5 ERTFIAIKPDGVQRGLVGEIIKRFEQKGFRLVGLKFIQASEDLLKEHYIDLKDRPFFSGLVKYMHSGPVVAMVWEG 80

HSP 2 Score: 51.2174 bits (121), Expect = 3.861e-29
Identity = 31/80 (38.75%), Postives = 43/80 (53.75%), Query Frame = 3 
Query:  618 VIAMVWEGEXSITYGRKLIGATDPQKSEPGNIRGDLAGVV*RNSFMGV-MARDRKDEINCGSNPKTG*LHKH-AEKWVYE 851
            V+AMVWEG   +  GR ++G T+P  S+PG IRGD    V RN   G       + EI+    P+    +K  A+ W+YE
Sbjct:   73 VVAMVWEGLNVVKTGRVMLGETNPADSKPGTIRGDFCIQVGRNIIHGSDSVESAEKEISLWFQPEELVDYKSCAQNWIYE 152
[Close]
BLAST of EY705313 vs. ExPASy Swiss-Prot
Match: NDKB_PIG (Nucleoside diphosphate kinase B OS=Sus scrofa GN=NME2 PE=2 SV=1)

HSP 1 Score: 99.3673 bits (246), Expect = 5.028e-29
Identity = 46/76 (60.53%), Postives = 61/76 (80.26%), Query Frame = 1 
Query:  415 ERTFIAIKPDGVQRGLISEIISRFERKGFKLVAIKIVVPSKEFAQKHYHDLKERPFFNGLCEFLSSGP--SLPWYG 636
            ERTFIA+KPDGVQRGL+ EII RFE+KGF+LVA+K +  S+E  ++HY DLK+RPFF GL +++ SGP  ++ W G
Sbjct:    5 ERTFIAVKPDGVQRGLVGEIIKRFEQKGFRLVALKFLQASEELLKQHYIDLKDRPFFPGLVKYMGSGPVVAMVWEG 80

HSP 2 Score: 50.0618 bits (118), Expect = 5.028e-29
Identity = 31/80 (38.75%), Postives = 43/80 (53.75%), Query Frame = 3 
Query:  618 VIAMVWEGEXSITYGRKLIGATDPQKSEPGNIRGDLAGVV*RNSFMGV-MARDRKDEINCGSNPKTG*LHKH-AEKWVYE 851
            V+AMVWEG   +  GR ++G T+P  S+PG IRGD    V RN   G    +  + EI+    P+    +K  A  W+YE
Sbjct:   73 VVAMVWEGLNVVKTGRVMLGETNPADSKPGTIRGDFCIQVGRNIIHGSDSVKSAEKEISLWFKPEELVEYKSCAFDWIYE 152
[Close]
BLAST of EY705313 vs. ExPASy Swiss-Prot
Match: NDKA1_BOVIN (Nucleoside diphosphate kinase A 1 OS=Bos taurus GN=NME1-1 PE=1 SV=3)

HSP 1 Score: 97.8265 bits (242), Expect = 8.524e-29
Identity = 47/76 (61.84%), Postives = 61/76 (80.26%), Query Frame = 1 
Query:  415 ERTFIAIKPDGVQRGLISEIISRFERKGFKLVAIKIVVPSKEFAQKHYHDLKERPFFNGLCEFLSSGP--SLPWYG 636
            ERTFIAIKPDGVQRGLI EII RFE+KGF+LVA+K +  S++  ++HY DLK+RPFF GL +++ SGP  ++ W G
Sbjct:    5 ERTFIAIKPDGVQRGLIGEIIKRFEQKGFRLVAMKFMRASEDLLKEHYIDLKDRPFFAGLVKYMHSGPVVAMVWEG 80

HSP 2 Score: 50.8322 bits (120), Expect = 8.524e-29
Identity = 31/80 (38.75%), Postives = 43/80 (53.75%), Query Frame = 3 
Query:  618 VIAMVWEGEXSITYGRKLIGATDPQKSEPGNIRGDLAGVV*RNSFMGV-MARDRKDEINCGSNPKTG*LHKH-AEKWVYE 851
            V+AMVWEG   +  GR ++G T+P  S+PG IRGD    V RN   G       + EI    +P+    +K  A+ W+YE
Sbjct:   73 VVAMVWEGLNVVKTGRVMLGETNPADSKPGTIRGDFCIQVGRNIIHGSDSVESAEKEIALWFHPEELVNYKSCAQNWIYE 152
[Close]
BLAST of EY705313 vs. ExPASy Swiss-Prot
Match: NDK_SYNY3 (Nucleoside diphosphate kinase OS=Synechocystis sp. (strain PCC 6803) GN=ndk PE=1 SV=1)

HSP 1 Score: 97.4413 bits (241), Expect = 8.532e-29
Identity = 46/77 (59.74%), Postives = 59/77 (76.62%), Query Frame = 1 
Query:  412 MERTFIAIKPDGVQRGLISEIISRFERKGFKLVAIKIVVPSKEFAQKHYHDLKERPFFNGLCEFLSSGP--SLPWYG 636
            MERTFI IKPDGVQR LI EI+ RFE+KGFKLVA+K++  S+E A+KHY  L ++PFF+GL  F+ S P  ++ W G
Sbjct:    1 MERTFIMIKPDGVQRQLIGEIVGRFEKKGFKLVAMKVMTVSQELAEKHYEALNDKPFFSGLVNFICSSPVVAMVWEG 77

HSP 2 Score: 51.2174 bits (121), Expect = 8.532e-29
Identity = 24/47 (51.06%), Postives = 30/47 (63.83%), Query Frame = 3 
Query:  618 VIAMVWEGEXSITYGRKLIGATDPQKSEPGNIRGDLAGVV*RNSFMG 758
            V+AMVWEG   ++  R++IGATDP  + PG IRGD    V RN   G
Sbjct:   70 VVAMVWEGNSIVSTSRQMIGATDPHAAAPGTIRGDYGVSVGRNIIHG 116
[Close]
BLAST of EY705313 vs. ExPASy Swiss-Prot
Match: NDK_THEEB (Nucleoside diphosphate kinase OS=Thermosynechococcus elongatus (strain BP-1) GN=ndk PE=3 SV=1)

HSP 1 Score: 94.3597 bits (233), Expect = 8.532e-29
Identity = 43/78 (55.13%), Postives = 61/78 (78.21%), Query Frame = 1 
Query:  412 MERTFIAIKPDGVQRGLISEIISRFERKGFKLVAIKIVVPSKEFAQKHYHDLKERPFFNGLCEFLSSGP--SLPWYGK 639
            MERTF+AIKPDGVQRGL+  II RFE+KG+ LV +K++  S+E A++HY + K++PFF GL  F++SGP  ++ W G+
Sbjct:    1 MERTFLAIKPDGVQRGLVGTIIQRFEQKGYTLVGLKLMRVSRELAEQHYGEHKDKPFFPGLVNFITSGPVVAMVWEGR 78

HSP 2 Score: 54.299 bits (129), Expect = 8.532e-29
Identity = 27/47 (57.45%), Postives = 31/47 (65.96%), Query Frame = 3 
Query:  618 VIAMVWEGEXSITYGRKLIGATDPQKSEPGNIRGDLAGVV*RNSFMG 758
            V+AMVWEG   I   RKLIGAT+P  +EPG +RGD A  V RN   G
Sbjct:   70 VVAMVWEGRGVIANARKLIGATNPLNAEPGTLRGDFAVDVGRNVIHG 116
[Close]
BLAST of EY705313 vs. ExPASy Swiss-Prot
Match: NDK_ANAVT (Nucleoside diphosphate kinase OS=Anabaena variabilis (strain ATCC 29413 / PCC 7937) GN=ndk PE=3 SV=1)

HSP 1 Score: 95.5153 bits (236), Expect = 1.270e-28
Identity = 47/77 (61.04%), Postives = 58/77 (75.32%), Query Frame = 1 
Query:  412 MERTFIAIKPDGVQRGLISEIISRFERKGFKLVAIKIVVPSKEFAQKHYHDLKERPFFNGLCEFLSSGP--SLPWYG 636
            MERTF+AIKPDGVQRGL+ EII RFE KGF LV +K +  SKE A++HY   +ERPFF  L EF++SGP  ++ W G
Sbjct:    1 MERTFLAIKPDGVQRGLVGEIIRRFETKGFTLVGLKFLQVSKELAEQHYGVHRERPFFPSLVEFITSGPVVAMVWEG 77

HSP 2 Score: 52.373 bits (124), Expect = 1.270e-28
Identity = 25/47 (53.19%), Postives = 31/47 (65.96%), Query Frame = 3 
Query:  618 VIAMVWEGEXSITYGRKLIGATDPQKSEPGNIRGDLAGVV*RNSFMG 758
            V+AMVWEG+  I   RK+IGAT+P  +EPG IRGD    + RN   G
Sbjct:   70 VVAMVWEGDGVIASARKIIGATNPLTAEPGTIRGDFGINIGRNLIHG 116

HSP 3 Score: 20.4014 bits (41), Expect = 1.270e-28
Identity = 7/10 (70.00%), Postives = 8/10 (80.00%), Query Frame = 2 
Query:  740 KKLIHGSDGP 769
            + LIHGSD P
Sbjct:  111 RNLIHGSDAP 120
The following BLAST results are available for this feature:
BLAST of EY705313 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 500
Match NameE-valueIdentityDescription
NDK_SYNE72.969e-2969.57Nucleoside diphosphate kinase OS=Synechococcus elo... [more]
NDK1_ARATH2.969e-2955.13Nucleoside diphosphate kinase 1 OS=Arabidopsis tha... [more]
NDK_SYNP23.439e-2956.41Nucleoside diphosphate kinase OS=Synechococcus sp.... [more]
NDKB_RAT3.861e-2961.04Nucleoside diphosphate kinase B OS=Rattus norvegic... [more]
NDKA_RAT3.861e-2959.21Nucleoside diphosphate kinase A OS=Rattus norvegic... [more]
NDKB_PIG5.028e-2960.53Nucleoside diphosphate kinase B OS=Sus scrofa GN=N... [more]
NDKA1_BOVIN8.524e-2961.84Nucleoside diphosphate kinase A 1 OS=Bos taurus GN... [more]
NDK_SYNY38.532e-2959.74Nucleoside diphosphate kinase OS=Synechocystis sp.... [more]
NDK_THEEB8.532e-2955.13Nucleoside diphosphate kinase OS=Thermosynechococc... [more]
NDK_ANAVT1.270e-2861.04Nucleoside diphosphate kinase OS=Anabaena variabil... [more]

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Properties
Property NameValue
Genbank descriptionCS00-C3-701-042-B10-CT.F Sweet orange fruit, development stadium (2 of 6) Citrus sinensis cDNA, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>EY705313 ID=EY705313; Name=EY705313; organism=Citrus sinensis; type=EST; length=922bp
TGCAGACCCACGCGTCCGGTTTTCTGATTATCTTTATGTCTGTTTTGCAA
GTTGTTACTGACTGCACTTCATATTATGAAAATCTGCAAGCCAAAGTTTC
TTATCAATCTGATAAAACCTTCACTCTCTTTACTAAAGGTGGATCTAAAA
GAAGAAATTAATATTGTTGATAAAAGCTTGGATAACCATTTCTTCACAAT
TTTATCTCTTCAAGAGAACAATAATAGGAAATATTTTATGTAACTTTATG
ACATCATAGCTTTGAATATTAATTGCACGAAGTTGCGCCTTCCTTTTTCC
TCCAAAATCTCTGATCTTAATTTTTCCTTTGTGCTTTTCTTTATTATTGC
AGCTTACATGCTCCAGGAGCAGGAAGTACATGCTGCAGAGGTACTTTAAT
CACTGATCTAAATGGAACGCACTTTCATTGCCATCAAGCCAGATGGGGTG
CAAAGAGGACTGATTTCAGAAATCATCTCTCGCTTTGAGCGCAAAGGCTT
TAAGCTTGTGGCCATTAAGATAGTAGTTCCATCAAAAGAGTTTGCACAAA
AACATTATCATGATCTGAAGGAAAGACCCTTCTTTAATGGCCTGTGCGAG
TTCCTTAGCTCTGGTCCGTCATTGCCATGGTATGGGAAGGAGAGGNNAGT
ATTACGTATGGTCGCAAACTCATTGGAGCCACAGATCCTCAGAAATCAGA
ACCTGGAAACATCAGAGGCGATCTAGCTGGTGTTGTTTGAAGAAACTCAT
TCATGGGAGTGATGGCCCGAGACCGCAAGGATGAAATAAATTGTGGGTCC
AACCCCAAGACTGGTTAATTACACAAGCATGCAGAGAAGTGGGTCTATGA
ATCCACTGATTACATTTTTCTCTACTTTATTAAAACTAAGGCCTACTGAA
TAGGGGATAAAAGCATAATGGT
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