EY705313

Overview
NameEY705313
Unique NameEY705313
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length922
Libraries
Library NameType
Sweet orange fruit, development stadium (2 of 6)cdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of EY705313 vs. ExPASy Swiss-Prot
Match: NDK_CHICK (Nucleoside diphosphate kinase OS=Gallus gallus PE=2 SV=1)

HSP 1 Score: 98.2117 bits (243), Expect = 3.189e-28
Identity = 46/76 (60.53%), Postives = 62/76 (81.58%), Query Frame = 1 
Query:  415 ERTFIAIKPDGVQRGLISEIISRFERKGFKLVAIKIVVPSKEFAQKHYHDLKERPFFNGLCEFLSSGP--SLPWYG 636
            ERTFIAIKPDGVQRGL+ EII RFE+KGF+LVA+K V  S++  ++HY DLK+RPF+ GL ++++SGP  ++ W G
Sbjct:    6 ERTFIAIKPDGVQRGLVGEIIKRFEQKGFRLVAMKFVHASEDLLKQHYIDLKDRPFYPGLVKYMNSGPVVAMVWEG 81

HSP 2 Score: 48.521 bits (114), Expect = 3.189e-28
Identity = 31/80 (38.75%), Postives = 41/80 (51.25%), Query Frame = 3 
Query:  618 VIAMVWEGEXSITYGRKLIGATDPQKSEPGNIRGDLAGVV*RNSFMGV-MARDRKDEINCGSNPKTG*LHKH-AEKWVYE 851
            V+AMVWEG   +  GR ++G T+P  S+PG IRGD    V RN   G       + EI+    P     ++  A  WVYE
Sbjct:   74 VVAMVWEGLNVVKTGRVMLGETNPADSKPGTIRGDFCIQVGRNIIHGSDSVESAQKEISLWFKPAELIDYRSCAHDWVYE 153
[Close]
BLAST of EY705313 vs. ExPASy Swiss-Prot
Match: NDK_MICAN (Nucleoside diphosphate kinase OS=Microcystis aeruginosa (strain NIES-843) GN=ndk PE=3 SV=1)

HSP 1 Score: 90.8929 bits (224), Expect = 3.193e-28
Identity = 44/78 (56.41%), Postives = 56/78 (71.79%), Query Frame = 1 
Query:  412 MERTFIAIKPDGVQRGLISEIISRFERKGFKLVAIKIVVPSKEFAQKHYHDLKERPFFNGLCEFLSSGP--SLPWYGK 639
            MERTF+ IKPDGVQR L+ EII RFE KGF LV +K++  S E A+KHY   KERPFF  L +F++S P  ++ W G+
Sbjct:    1 MERTFLMIKPDGVQRNLVGEIIQRFETKGFTLVGLKMMQVSSELAEKHYAVHKERPFFRSLVDFITSSPVVAMVWQGE 78

HSP 2 Score: 55.8398 bits (133), Expect = 3.193e-28
Identity = 30/59 (50.85%), Postives = 37/59 (62.71%), Query Frame = 3 
Query:  618 VIAMVWEGEXSITYGRKLIGATDPQKSEPGNIRGDLAGVV*RNSFMGVMARD-RKDEIN 791
            V+AMVW+GE  I   RK+IGAT+P  +EPG IRGD    V RN   G    D  KDE++
Sbjct:   70 VVAMVWQGEGVIASARKIIGATNPLNAEPGTIRGDFGISVGRNLIHGSDGPDTAKDEVS 128
[Close]
BLAST of EY705313 vs. ExPASy Swiss-Prot
Match: NDKA_PONAB (Nucleoside diphosphate kinase A OS=Pongo abelii GN=NME1 PE=2 SV=1)

HSP 1 Score: 96.6709 bits (239), Expect = 7.039e-28
Identity = 45/76 (59.21%), Postives = 60/76 (78.95%), Query Frame = 1 
Query:  415 ERTFIAIKPDGVQRGLISEIISRFERKGFKLVAIKIVVPSKEFAQKHYHDLKERPFFNGLCEFLSSGP--SLPWYG 636
            ERTFIAIKPDGVQRGL+ EII RFE+KGF+LV +K +  S++  ++HY DLK+RPFF GL +++ SGP  ++ W G
Sbjct:    5 ERTFIAIKPDGVQRGLVGEIIKRFEQKGFRLVGLKFMQASEDLLKEHYVDLKDRPFFAGLVKYMHSGPVVAMVWEG 80

HSP 2 Score: 48.9062 bits (115), Expect = 7.039e-28
Identity = 30/80 (37.50%), Postives = 42/80 (52.50%), Query Frame = 3 
Query:  618 VIAMVWEGEXSITYGRKLIGATDPQKSEPGNIRGDLAGVV*RNSFMGV-MARDRKDEINCGSNPKTG*LHKH-AEKWVYE 851
            V+AMVWEG   +  GR ++G T+P  S+PG IRGD    V RN   G       + EI    +P+    +   A+ W+YE
Sbjct:   73 VVAMVWEGLNVVKTGRVMLGETNPADSKPGTIRGDFCIQVGRNIIHGSDSVESAEKEIGLWFHPEELVDYTSCAQNWIYE 152
[Close]
BLAST of EY705313 vs. ExPASy Swiss-Prot
Match: NDKA_HUMAN (Nucleoside diphosphate kinase A OS=Homo sapiens GN=NME1 PE=1 SV=1)

HSP 1 Score: 96.6709 bits (239), Expect = 7.039e-28
Identity = 45/76 (59.21%), Postives = 60/76 (78.95%), Query Frame = 1 
Query:  415 ERTFIAIKPDGVQRGLISEIISRFERKGFKLVAIKIVVPSKEFAQKHYHDLKERPFFNGLCEFLSSGP--SLPWYG 636
            ERTFIAIKPDGVQRGL+ EII RFE+KGF+LV +K +  S++  ++HY DLK+RPFF GL +++ SGP  ++ W G
Sbjct:    5 ERTFIAIKPDGVQRGLVGEIIKRFEQKGFRLVGLKFMQASEDLLKEHYVDLKDRPFFAGLVKYMHSGPVVAMVWEG 80

HSP 2 Score: 48.9062 bits (115), Expect = 7.039e-28
Identity = 30/80 (37.50%), Postives = 42/80 (52.50%), Query Frame = 3 
Query:  618 VIAMVWEGEXSITYGRKLIGATDPQKSEPGNIRGDLAGVV*RNSFMGV-MARDRKDEINCGSNPKTG*LHKH-AEKWVYE 851
            V+AMVWEG   +  GR ++G T+P  S+PG IRGD    V RN   G       + EI    +P+    +   A+ W+YE
Sbjct:   73 VVAMVWEGLNVVKTGRVMLGETNPADSKPGTIRGDFCIQVGRNIIHGSDSVESAEKEIGLWFHPEELVDYTSCAQNWIYE 152
[Close]
BLAST of EY705313 vs. ExPASy Swiss-Prot
Match: NDK_SYNJB (Nucleoside diphosphate kinase OS=Synechococcus sp. GN=ndk PE=3 SV=1)

HSP 1 Score: 95.1301 bits (235), Expect = 7.046e-28
Identity = 45/78 (57.69%), Postives = 60/78 (76.92%), Query Frame = 1 
Query:  412 MERTFIAIKPDGVQRGLISEIISRFERKGFKLVAIKIVVPSKEFAQKHYHDLKERPFFNGLCEFLSSGP--SLPWYGK 639
            MERTFIAIKPDGVQRGL+  II R E +G++LV +K+V  S+E A+ HY + KERPFF+ L +F++SGP  ++ W GK
Sbjct:    1 MERTFIAIKPDGVQRGLVGTIIQRLESRGYQLVGLKLVQVSQELAEAHYAEHKERPFFHSLVKFITSGPVVAMVWQGK 78

HSP 2 Score: 50.447 bits (119), Expect = 7.046e-28
Identity = 24/47 (51.06%), Postives = 31/47 (65.96%), Query Frame = 3 
Query:  618 VIAMVWEGEXSITYGRKLIGATDPQKSEPGNIRGDLAGVV*RNSFMG 758
            V+AMVW+G+  I   RKLIG T+P ++EPG IRGD    + RN   G
Sbjct:   70 VVAMVWQGKGVIAAARKLIGKTNPLEAEPGTIRGDFGIDIGRNLIHG 116
[Close]
BLAST of EY705313 vs. ExPASy Swiss-Prot
Match: NDK2_ARATH (Nucleoside diphosphate kinase II, chloroplastic OS=Arabidopsis thaliana GN=NDPK2 PE=1 SV=2)

HSP 1 Score: 93.5893 bits (231), Expect = 1.177e-27
Identity = 52/135 (38.52%), Postives = 82/135 (60.74%), Query Frame = 1 
Query:  259 ALNINCTKLRLPFSSKISDLNFSFVLFFIIAAYMLQEQEVHAAE-------VL*SLI*MERTFIAIKPDGVQRGLISEIISRFERKGFKLVAIKIVVPSKEFAQKHYHDLKERPFFNGLCEFLSSGP--SLPWYG 636
            +LN +C+  R+ F + ++     F LF   +A   + +   +AE       ++ S+  +E T+I +KPDG+QRGL+ EIISRFE+KGFKL+ +K+    KE A++HY DL  + FF  L E+++SGP   + W G
Sbjct:   25 SLNPHCSPARVNFRTALAAFRPQFRLFSRNSASRRRLRASSSAESGIFLPHLVASMEDVEETYIMVKPDGIQRGLVGEIISRFEKKGFKLIGLKMFQCPKELAEEHYKDLSAKSFFPNLIEYITSGPVVCMAWEG 159

HSP 2 Score: 51.2174 bits (121), Expect = 1.177e-27
Identity = 25/47 (53.19%), Postives = 29/47 (61.70%), Query Frame = 3 
Query:  618 VIAMVWEGEXSITYGRKLIGATDPQKSEPGNIRGDLAGVV*RNSFMG 758
            V+ M WEG   +   RKLIG TDP ++EPG IRGDLA    RN   G
Sbjct:  152 VVCMAWEGVGVVASARKLIGKTDPLQAEPGTIRGDLAVQTGRNIVHG 198
[Close]
BLAST of EY705313 vs. ExPASy Swiss-Prot
Match: NDKB_CANFA (Nucleoside diphosphate kinase B OS=Canis familiaris GN=NME2 PE=2 SV=1)

HSP 1 Score: 94.7449 bits (234), Expect = 1.193e-27
Identity = 43/76 (56.58%), Postives = 61/76 (80.26%), Query Frame = 1 
Query:  415 ERTFIAIKPDGVQRGLISEIISRFERKGFKLVAIKIVVPSKEFAQKHYHDLKERPFFNGLCEFLSSGP--SLPWYG 636
            ERTFIAIKPDGVQRGL+ +I+ RFE+KGF+LVA+K +  S++  ++HY DLK+RPF+ GL +++ SGP  ++ W G
Sbjct:    5 ERTFIAIKPDGVQRGLVGDIVKRFEQKGFRLVAMKFLRASEDLLKEHYIDLKDRPFYPGLVKYMHSGPVVAMVWEG 80

HSP 2 Score: 50.0618 bits (118), Expect = 1.193e-27
Identity = 31/80 (38.75%), Postives = 43/80 (53.75%), Query Frame = 3 
Query:  618 VIAMVWEGEXSITYGRKLIGATDPQKSEPGNIRGDLAGVV*RNSFMGV-MARDRKDEINCGSNPKTG*LHKH-AEKWVYE 851
            V+AMVWEG   +  GR ++G T+P  S+PG IRGD    V RN   G    +  + EI+    P+    +K  A  W+YE
Sbjct:   73 VVAMVWEGLNVVKTGRMMLGETNPADSKPGTIRGDFCIQVGRNIIHGSDSVKSAEKEISLWFKPEELVDYKSCAFDWIYE 152
[Close]
BLAST of EY705313 vs. ExPASy Swiss-Prot
Match: NDKA1_XENLA (Nucleoside diphosphate kinase A1 OS=Xenopus laevis PE=2 SV=1)

HSP 1 Score: 97.0561 bits (240), Expect = 2.020e-27
Identity = 46/76 (60.53%), Postives = 61/76 (80.26%), Query Frame = 1 
Query:  415 ERTFIAIKPDGVQRGLISEIISRFERKGFKLVAIKIVVPSKEFAQKHYHDLKERPFFNGLCEFLSSGP--SLPWYG 636
            ERTFIAIKPDGVQRGL+ +II RFE+KGF+LVA+K    S++  ++HY DLK+RPF+ GL E++SSGP  ++ W G
Sbjct:    6 ERTFIAIKPDGVQRGLMGDIIKRFEQKGFRLVAMKFQQASQDLLRQHYIDLKDRPFYPGLVEYMSSGPVLAMVWEG 81

HSP 2 Score: 46.9802 bits (110), Expect = 2.020e-27
Identity = 23/47 (48.94%), Postives = 29/47 (61.70%), Query Frame = 3 
Query:  618 VIAMVWEGEXSITYGRKLIGATDPQKSEPGNIRGDLAGVV*RNSFMG 758
            V+AMVWEG   +  GR ++G T+P  S+PG IRGD    V RN   G
Sbjct:   74 VLAMVWEGLNVVKTGRVMLGETNPADSKPGTIRGDFCIQVGRNIIHG 120
[Close]
BLAST of EY705313 vs. ExPASy Swiss-Prot
Match: NDK_GEOKA (Nucleoside diphosphate kinase OS=Geobacillus kaustophilus GN=ndk PE=3 SV=1)

HSP 1 Score: 93.2041 bits (230), Expect = 3.781e-27
Identity = 43/77 (55.84%), Postives = 60/77 (77.92%), Query Frame = 1 
Query:  415 ERTFIAIKPDGVQRGLISEIISRFERKGFKLVAIKIVVPSKEFAQKHYHDLKERPFFNGLCEFLSSGP--SLPWYGK 639
            ERTFI +KPDGVQR LI EI++RFE+KGF+LV  K++  S+E A++HY + KERPFF  L +F++SGP  ++ W G+
Sbjct:    3 ERTFIMVKPDGVQRNLIGEIVARFEKKGFQLVGAKLMQVSRELAEQHYAEHKERPFFGELVDFITSGPVFAMVWEGE 79

HSP 2 Score: 49.2914 bits (116), Expect = 3.781e-27
Identity = 23/47 (48.94%), Postives = 30/47 (63.83%), Query Frame = 3 
Query:  618 VIAMVWEGEXSITYGRKLIGATDPQKSEPGNIRGDLAGVV*RNSFMG 758
            V AMVWEGE  I   R+++G T+PQ++ PG IRGD    V +N   G
Sbjct:   71 VFAMVWEGENVIAAARQMMGKTNPQEAAPGTIRGDFGLTVGKNVIHG 117

HSP 3 Score: 20.7866 bits (42), Expect = 3.781e-27
Identity = 7/11 (63.64%), Postives = 9/11 (81.82%), Query Frame = 2 
Query:  740 KKLIHGSDGPR 772
            K +IHGSD P+
Sbjct:  112 KNVIHGSDSPQ 122
[Close]
BLAST of EY705313 vs. ExPASy Swiss-Prot
Match: NDK_BACHD (Nucleoside diphosphate kinase OS=Bacillus halodurans GN=ndk PE=3 SV=1)

HSP 1 Score: 92.4337 bits (228), Expect = 4.472e-27
Identity = 43/78 (55.13%), Postives = 59/78 (75.64%), Query Frame = 1 
Query:  412 MERTFIAIKPDGVQRGLISEIISRFERKGFKLVAIKIVVPSKEFAQKHYHDLKERPFFNGLCEFLSSGP--SLPWYGK 639
            MERT++ IKPDGVQR LI EI+SRFE+KGF L+  K++  +KE A+ HY + KERPFF  L +F++SGP  ++ W G+
Sbjct:    1 MERTYLMIKPDGVQRNLIGEIVSRFEKKGFTLIGAKLMTVTKEQAETHYAEHKERPFFGELVDFITSGPVFAMVWEGE 78

HSP 2 Score: 50.447 bits (119), Expect = 4.472e-27
Identity = 25/47 (53.19%), Postives = 29/47 (61.70%), Query Frame = 3 
Query:  618 VIAMVWEGEXSITYGRKLIGATDPQKSEPGNIRGDLAGVV*RNSFMG 758
            V AMVWEGE  I   RK++GAT+P  +EPG IRGD    V  N   G
Sbjct:   70 VFAMVWEGENVIATARKMMGATNPADAEPGTIRGDFGVQVAMNVIHG 116
The following BLAST results are available for this feature:
BLAST of EY705313 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 500
Match NameE-valueIdentityDescription
NDK_CHICK3.189e-2860.53Nucleoside diphosphate kinase OS=Gallus gallus PE=... [more]
NDK_MICAN3.193e-2856.41Nucleoside diphosphate kinase OS=Microcystis aerug... [more]
NDKA_PONAB7.039e-2859.21Nucleoside diphosphate kinase A OS=Pongo abelii GN... [more]
NDKA_HUMAN7.039e-2859.21Nucleoside diphosphate kinase A OS=Homo sapiens GN... [more]
NDK_SYNJB7.046e-2857.69Nucleoside diphosphate kinase OS=Synechococcus sp.... [more]
NDK2_ARATH1.177e-2738.52Nucleoside diphosphate kinase II, chloroplastic OS... [more]
NDKB_CANFA1.193e-2756.58Nucleoside diphosphate kinase B OS=Canis familiari... [more]
NDKA1_XENLA2.020e-2760.53Nucleoside diphosphate kinase A1 OS=Xenopus laevis... [more]
NDK_GEOKA3.781e-2755.84Nucleoside diphosphate kinase OS=Geobacillus kaust... [more]
NDK_BACHD4.472e-2755.13Nucleoside diphosphate kinase OS=Bacillus halodura... [more]

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Properties
Property NameValue
Genbank descriptionCS00-C3-701-042-B10-CT.F Sweet orange fruit, development stadium (2 of 6) Citrus sinensis cDNA, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>EY705313 ID=EY705313; Name=EY705313; organism=Citrus sinensis; type=EST; length=922bp
TGCAGACCCACGCGTCCGGTTTTCTGATTATCTTTATGTCTGTTTTGCAA
GTTGTTACTGACTGCACTTCATATTATGAAAATCTGCAAGCCAAAGTTTC
TTATCAATCTGATAAAACCTTCACTCTCTTTACTAAAGGTGGATCTAAAA
GAAGAAATTAATATTGTTGATAAAAGCTTGGATAACCATTTCTTCACAAT
TTTATCTCTTCAAGAGAACAATAATAGGAAATATTTTATGTAACTTTATG
ACATCATAGCTTTGAATATTAATTGCACGAAGTTGCGCCTTCCTTTTTCC
TCCAAAATCTCTGATCTTAATTTTTCCTTTGTGCTTTTCTTTATTATTGC
AGCTTACATGCTCCAGGAGCAGGAAGTACATGCTGCAGAGGTACTTTAAT
CACTGATCTAAATGGAACGCACTTTCATTGCCATCAAGCCAGATGGGGTG
CAAAGAGGACTGATTTCAGAAATCATCTCTCGCTTTGAGCGCAAAGGCTT
TAAGCTTGTGGCCATTAAGATAGTAGTTCCATCAAAAGAGTTTGCACAAA
AACATTATCATGATCTGAAGGAAAGACCCTTCTTTAATGGCCTGTGCGAG
TTCCTTAGCTCTGGTCCGTCATTGCCATGGTATGGGAAGGAGAGGNNAGT
ATTACGTATGGTCGCAAACTCATTGGAGCCACAGATCCTCAGAAATCAGA
ACCTGGAAACATCAGAGGCGATCTAGCTGGTGTTGTTTGAAGAAACTCAT
TCATGGGAGTGATGGCCCGAGACCGCAAGGATGAAATAAATTGTGGGTCC
AACCCCAAGACTGGTTAATTACACAAGCATGCAGAGAAGTGGGTCTATGA
ATCCACTGATTACATTTTTCTCTACTTTATTAAAACTAAGGCCTACTGAA
TAGGGGATAAAAGCATAATGGT
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