EY705312 | Citrus Genome Database

Overview


Name	EY705312
Unique Name	EY705312
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	888

Libraries


Library Name	Type
Sweet orange fruit, development stadium (2 of 6)	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Alignments

Feature Name	Type	Location	Analysis
EY705312_ssr32	microsatellite	EY705312_ssr32:32..47.	BLAST: Citrus ESTs to Prunus persica proteins V1

Homology

[Close]

BLAST of EY705312 vs. ExPASy Swiss-Prot
Match: EXPA2_ORYSJ (Expansin-A2 OS=Oryza sativa subsp. japonica GN=EXPA2 PE=2 SV=2)

HSP 1 Score: 274.633 bits (701), Expect = 3.207e-82
Identity = 122/165 (73.94%), Postives = 139/165 (84.24%), Query Frame = 1

Query:  172 GPWQSAHATFYGGSDASGTMGGACGYGNLYSQGYGVNTAALXTALFNNGLSCGACFELKCGGDPQWCNPGNPAILITATNFCPPNFAQPSDNGGWCNPPRPHFDLAMPMFLKLAQYRAGIVPVSYRRVPCRKRGGIRFTINGFRYFNLVLVTNVAGAGDIVRVSV 666
            G WQSAHATFYGG DASGTMGGACGYGNLYS GYG NTAAL T LFN+G +CG+C+EL+C  D QWC PG+  + +TATN CPPN+A P+D+GGWCNPPRPHFD+A P FL++  YRAGIVPVSYRRVPC K+GGIRFTING  YFNLVLVTNVAG GD+  VS+
Sbjct:   27 GSWQSAHATFYGGGDASGTMGGACGYGNLYSTGYGTNTAALSTVLFNDGAACGSCYELRCDNDGQWCLPGS--VTVTATNLCPPNYALPNDDGGWCNPPRPHFDMAEPAFLQIGVYRAGIVPVSYRRVPCVKKGGIRFTINGHSYFNLVLVTNVAGPGDVQSVSI 189

HSP 2 Score: 52.7582 bits (125), Expect = 3.207e-82
Identity = 24/36 (66.67%), Postives = 27/36 (75.00%), Query Frame = 3

Query:  675 NTQWLSMSRNWGQNWQSNSQLVGQALSFRSTGSDRR 782
            +T W  MSRNWGQNWQSNS L GQ+LSF+   SD R
Sbjct:  193 STGWQPMSRNWGQNWQSNSYLDGQSLSFQVAVSDGR 228

[Close]

BLAST of EY705312 vs. ExPASy Swiss-Prot
Match: EXPA4_ORYSJ (Expansin-A4 OS=Oryza sativa subsp. japonica GN=EXPA4 PE=2 SV=1)

HSP 1 Score: 272.707 bits (696), Expect = 7.118e-82
Identity = 123/165 (74.55%), Postives = 140/165 (84.85%), Query Frame = 1

Query:  172 GPWQSAHATFYGGSDASGTMGGACGYGNLYSQGYGVNTAALXTALFNNGLSCGACFELKCGGDPQWCNPGNPAILITATNFCPPNFAQPSDNGGWCNPPRPHFDLAMPMFLKLAQYRAGIVPVSYRRVPCRKRGGIRFTINGFRYFNLVLVTNVAGAGDIVRVSV 666
            G WQSAHATFYGG DASGTMGGACGYGNLYSQGYG NTAAL TALFN+G +CG+C+EL+C      C PG+  I +TATNFCPPN+  PSD+GGWCNPPRPHFD+A P FL +AQYRAGIVPVS+RRVPC K+GG+RFT+NG  YFNLVLVTNVAGAGD+  VS+
Sbjct:   22 GGWQSAHATFYGGGDASGTMGGACGYGNLYSQGYGTNTAALSTALFNDGAACGSCYELRCDNAGSSCLPGS--ITVTATNFCPPNYGLPSDDGGWCNPPRPHFDMAEPAFLHIAQYRAGIVPVSFRRVPCVKKGGVRFTVNGHSYFNLVLVTNVAGAGDVRSVSI 184

HSP 2 Score: 53.5286 bits (127), Expect = 7.118e-82
Identity = 24/35 (68.57%), Postives = 27/35 (77.14%), Query Frame = 3

Query:  678 TQWLSMSRNWGQNWQSNSQLVGQALSFRSTGSDRR 782
            T W  MSRNWGQNWQSN+ L GQ+LSF+ T SD R
Sbjct:  189 TGWQPMSRNWGQNWQSNAFLDGQSLSFQVTASDGR 223

[Close]

BLAST of EY705312 vs. ExPASy Swiss-Prot
Match: EXPA4_ORYSI (Expansin-A4 OS=Oryza sativa subsp. indica GN=EXPA4 PE=2 SV=1)

HSP 1 Score: 272.707 bits (696), Expect = 7.118e-82
Identity = 123/165 (74.55%), Postives = 140/165 (84.85%), Query Frame = 1

Query:  172 GPWQSAHATFYGGSDASGTMGGACGYGNLYSQGYGVNTAALXTALFNNGLSCGACFELKCGGDPQWCNPGNPAILITATNFCPPNFAQPSDNGGWCNPPRPHFDLAMPMFLKLAQYRAGIVPVSYRRVPCRKRGGIRFTINGFRYFNLVLVTNVAGAGDIVRVSV 666
            G WQSAHATFYGG DASGTMGGACGYGNLYSQGYG NTAAL TALFN+G +CG+C+EL+C      C PG+  I +TATNFCPPN+  PSD+GGWCNPPRPHFD+A P FL +AQYRAGIVPVS+RRVPC K+GG+RFT+NG  YFNLVLVTNVAGAGD+  VS+
Sbjct:   22 GGWQSAHATFYGGGDASGTMGGACGYGNLYSQGYGTNTAALSTALFNDGAACGSCYELRCDNAGSSCLPGS--ITVTATNFCPPNYGLPSDDGGWCNPPRPHFDMAEPAFLHIAQYRAGIVPVSFRRVPCVKKGGVRFTVNGHSYFNLVLVTNVAGAGDVRSVSI 184

HSP 2 Score: 53.5286 bits (127), Expect = 7.118e-82
Identity = 24/35 (68.57%), Postives = 27/35 (77.14%), Query Frame = 3

Query:  678 TQWLSMSRNWGQNWQSNSQLVGQALSFRSTGSDRR 782
            T W  MSRNWGQNWQSN+ L GQ+LSF+ T SD R
Sbjct:  189 TGWQPMSRNWGQNWQSNAFLDGQSLSFQVTASDGR 223

[Close]

BLAST of EY705312 vs. ExPASy Swiss-Prot
Match: EXPA2_ARATH (Expansin-A2 OS=Arabidopsis thaliana GN=EXPA2 PE=2 SV=2)

HSP 1 Score: 267.314 bits (682), Expect = 3.504e-81
Identity = 121/165 (73.33%), Postives = 140/165 (84.85%), Query Frame = 1

Query:  172 GPWQSAHATFYGGSDASGTMGGACGYGNLYSQGYGVNTAALXTALFNNGLSCGACFELKCGGDPQWCNPGNPAILITATNFCPPNFAQPSDNGGWCNPPRPHFDLAMPMFLKLAQYRAGIVPVSYRRVPCRKRGGIRFTINGFRYFNLVLVTNVAGAGDIVRVSV 666
            G W+  HATFYGG+DASGTMGGACGYGNL+SQGYG+ TAAL TALFN+G  CGACFEL+C  DP+WC PG+  I+++ATNFCPPNFA  +DNGGWCNPP  HFDLA P FL++AQYRAGIVPV++RRVPC K GGIRFTING  YF+LVL+TNV GAGDI  VS+
Sbjct:   30 GGWERGHATFYGGADASGTMGGACGYGNLHSQGYGLQTAALSTALFNSGQKCGACFELQCEDDPEWCIPGS--IIVSATNFCPPNFALANDNGGWCNPPLKHFDLAEPAFLQIAQYRAGIVPVAFRRVPCEKGGGIRFTINGNPYFDLVLITNVGGAGDIRAVSL 192

HSP 2 Score: 56.6102 bits (135), Expect = 3.504e-81
Identity = 25/34 (73.53%), Postives = 28/34 (82.35%), Query Frame = 3

Query:  681 QWLSMSRNWGQNWQSNSQLVGQALSFRSTGSDRR 782
            QW SMSRNWGQNWQSN+ L GQ+LSF+ T SD R
Sbjct:  199 QWQSMSRNWGQNWQSNTYLRGQSLSFQVTDSDGR 232

[Close]

BLAST of EY705312 vs. ExPASy Swiss-Prot
Match: EXPA8_ARATH (Expansin-A8 OS=Arabidopsis thaliana GN=EXPA8 PE=2 SV=1)

HSP 1 Score: 268.855 bits (686), Expect = 4.384e-80
Identity = 121/165 (73.33%), Postives = 135/165 (81.82%), Query Frame = 1

Query:  172 GPWQSAHATFYGGSDASGTMGGACGYGNLYSQGYGVNTAALXTALFNNGLSCGACFELKCGGDPQWCNPGNPAILITATNFCPPNFAQPSDNGGWCNPPRPHFDLAMPMFLKLAQYRAGIVPVSYRRVPCRKRGGIRFTINGFRYFNLVLVTNVAGAGDIVRVSV 666
            G WQ  HATFYGG DASGTMGGACGYGNLY QGYG NTAAL TALFNNGL+CGAC+E+KC  DP+WC      I +TATNFCPPN    +DNGGWCNPP  HFDLA P FL++AQYRAGIVPVS+RRVPC K+GGIRFTING  YFNLVL++NV GAGD+  VS+
Sbjct:   28 GGWQGGHATFYGGEDASGTMGGACGYGNLYGQGYGTNTAALSTALFNNGLTCGACYEMKCNDDPRWCL--GSTITVTATNFCPPNPGLSNDNGGWCNPPLQHFDLAEPAFLQIAQYRAGIVPVSFRRVPCMKKGGIRFTINGHSYFNLVLISNVGGAGDVHAVSI 190

HSP 2 Score: 50.8322 bits (120), Expect = 4.384e-80
Identity = 22/33 (66.67%), Postives = 26/33 (78.79%), Query Frame = 3

Query:  684 WLSMSRNWGQNWQSNSQLVGQALSFRSTGSDRR 782
            W +MSRNWGQNWQSNS +  Q+LSF+ T SD R
Sbjct:  198 WQAMSRNWGQNWQSNSYMNDQSLSFQVTTSDGR 230

HSP 3 Score: 21.9422 bits (45), Expect = 4.384e-80
Identity = 9/19 (47.37%), Postives = 12/19 (63.16%), Query Frame = 2

Query:  779 KTLTSXDVAPANXAVXQPF 835
            +TL S DVAP+N    Q +
Sbjct:  230 RTLVSNDVAPSNWQFGQTY 248

[Close]

BLAST of EY705312 vs. ExPASy Swiss-Prot
Match: EXPA5_ORYSJ (Expansin-A5 OS=Oryza sativa subsp. japonica GN=EXPA5 PE=2 SV=1)

HSP 1 Score: 268.47 bits (685), Expect = 1.884e-79
Identity = 124/169 (73.37%), Postives = 142/169 (84.02%), Query Frame = 1

Query:  169 GGPWQSAHATFYGGSDASGTMGGACGYGNLYSQGYGVNTAALXTALFNNGLSCGACFELKC---GGDPQWCNPGNPAILITATNFCPPNFAQPSDNGGWCNPPRPHFDLAMPMFLKLAQYRAGIVPVSYRRVPCRKRGGIRFTINGFRYFNLVLVTNVAGAGDIVRVSV 666
            GG W SAHATFYGG DASGTMGGACGYGNLYSQGYG NTAAL TALFNNGLSCGACFE++C   GG    C PG+  +++TATNFCPPN A PSD+GGWCNPPR HFD++ P+F ++A ++AGIVPVSYRRV C+K+GGIRFTING  YFNLVLVTNV GAGD+  V+V
Sbjct:   61 GGGWSSAHATFYGGGDASGTMGGACGYGNLYSQGYGTNTAALSTALFNNGLSCGACFEVRCDAGGGGSHSCLPGS--VVVTATNFCPPNNALPSDDGGWCNPPRAHFDMSQPVFQRIALFKAGIVPVSYRRVACQKKGGIRFTINGHSYFNLVLVTNVGGAGDVHAVAV 227

HSP 2 Score: 49.6766 bits (117), Expect = 1.884e-79
Identity = 22/33 (66.67%), Postives = 25/33 (75.76%), Query Frame = 3

Query:  684 WLSMSRNWGQNWQSNSQLVGQALSFRSTGSDRR 782
            W ++SRNWGQNWQS + L GQALSFR T  D R
Sbjct:  235 WQALSRNWGQNWQSAALLDGQALSFRVTTGDGR 267

[Close]

BLAST of EY705312 vs. ExPASy Swiss-Prot
Match: EXPA6_ORYSJ (Expansin-A6 OS=Oryza sativa subsp. japonica GN=EXPA6 PE=2 SV=1)

HSP 1 Score: 262.692 bits (670), Expect = 9.283e-79
Identity = 117/166 (70.48%), Postives = 137/166 (82.53%), Query Frame = 1

Query:  172 GPWQSAHATFYGGSDASGTMGGACGYGNLYSQGYGVNTAALXTALFNNGLSCGACFELKCGGDPQW-CNPGNPAILITATNFCPPNFAQPSDNGGWCNPPRPHFDLAMPMFLKLAQYRAGIVPVSYRRVPCRKRGGIRFTINGFRYFNLVLVTNVAGAGDIVRVSV 666
            G WQ  HATFYGG DASGTMGGACGYGNLYSQGYG +TAAL TALFN GLSCG+C+EL+C GD +  C PG   + +TATNFCPPN+A PSD GGWCNPPR HFDLA P FL++A++ AGIVPVS+RRV C ++GG+RFT+NG  YFNLVLVTNV GAGD+  ++V
Sbjct:   26 GQWQPGHATFYGGGDASGTMGGACGYGNLYSQGYGTSTAALSTALFNRGLSCGSCYELRCAGDHRRSCLPGGATVTVTATNFCPPNYALPSDGGGWCNPPRRHFDLAEPAFLRIARHAAGIVPVSFRRVACARKGGVRFTVNGHAYFNLVLVTNVGGAGDVRSLAV 191

HSP 2 Score: 53.1434 bits (126), Expect = 9.283e-79
Identity = 23/34 (67.65%), Postives = 26/34 (76.47%), Query Frame = 3

Query:  681 QWLSMSRNWGQNWQSNSQLVGQALSFRSTGSDRR 782
            +W  MSRNWGQNWQSN+ L G+ALSFR T  D R
Sbjct:  203 RWQPMSRNWGQNWQSNAYLDGKALSFRVTAGDGR 236

[Close]

BLAST of EY705312 vs. ExPASy Swiss-Prot
Match: EXPA5_ARATH (Expansin-A5 OS=Arabidopsis thaliana GN=EXPA5 PE=2 SV=1)

HSP 1 Score: 241.891 bits (616), Expect = 1.443e-73
Identity = 113/165 (68.48%), Postives = 131/165 (79.39%), Query Frame = 1

Query:  172 GPWQSAHATFYGGSDASGTMGGACGYGNLYSQGYGVNTAALXTALFNNGLSCGACFELKCGGDPQWCNPGNPAILITATNFCPPNFAQPSDNGGWCNPPRPHFDLAMPMFLKLAQYRAGIVPVSYRRVPCRKRGGIRFTINGFRYFNLVLVTNVAGAGDIVRVSV 666
            GPW +AHATFYGG DASGTMGGACGYGNLYSQGYG+ TAAL TALF+ GLSCGACFEL C  DPQWC  G  +I++TATNFCPP        GG C+PP  HFDL+ P++ K+A Y++GI+PV YRRV C++ GGIRFTING  YFNLVLVTNV GAGD+  VS+
Sbjct:   36 GPWINAHATFYGGGDASGTMGGACGYGNLYSQGYGLETAALSTALFDQGLSCGACFELMCVNDPQWCIKGR-SIVVTATNFCPP--------GGACDPPNHHFDLSQPIYEKIALYKSGIIPVMYRRVRCKRSGGIRFTINGHSYFNLVLVTNVGGAGDVHSVSM 191

HSP 2 Score: 56.6102 bits (135), Expect = 1.443e-73
Identity = 26/35 (74.29%), Postives = 28/35 (80.00%), Query Frame = 3

Query:  678 TQWLSMSRNWGQNWQSNSQLVGQALSFRSTGSDRR 782
            T+W  MSRNWGQNWQSNS L GQ+LSF  T SDRR
Sbjct:  196 TKWQLMSRNWGQNWQSNSYLNGQSLSFVVTTSDRR 230

[Close]

BLAST of EY705312 vs. ExPASy Swiss-Prot
Match: EXP17_ARATH (Putative expansin-A17 OS=Arabidopsis thaliana GN=EXPA17 PE=2 SV=1)

HSP 1 Score: 253.062 bits (645), Expect = 4.173e-73
Identity = 118/173 (68.21%), Postives = 137/173 (79.19%), Query Frame = 1

Query:  148 KIPGVFAGGPWQSAHATFYGGSDASGTMGGACGYGNLYSQGYGVNTAALXTALFNNGLSCGACFELKCGGD--PQWCNPGNPAILITATNFCPPNFAQPSDNGGWCNPPRPHFDLAMPMFLKLAQYRAGIVPVSYRRVPCRKRGGIRFTINGFRYFNLVLVTNVAGAGDIVRV 660
            KI  V AG  W  AHATFYGGSDASGTMGGACGYGNLY+ GY  NTAAL TALFN+G SCG C+++ C     PQWC  G  +I ITATNFCPPNFAQ SDNGGWCNPPRPHFD+A P FL +A+Y+AGIVP+ Y++V CR+ GG+RFTING  YF LVL++NVAG G+I +V
Sbjct:   20 KISSVSAG--WLQAHATFYGGSDASGTMGGACGYGNLYTDGYKTNTAALSTALFNDGKSCGGCYQILCDATKVPQWCLKGK-SITITATNFCPPNFAQASDNGGWCNPPRPHFDMAQPAFLTIAKYKAGIVPILYKKVGCRRSGGMRFTINGRNYFELVLISNVAGGGEISKV 189

HSP 2 Score: 43.8986 bits (102), Expect = 4.173e-73
Identity = 19/34 (55.88%), Postives = 26/34 (76.47%), Query Frame = 3

Query:  675 NTQWLSMSRNWGQNWQSNSQLVGQALSFRSTGSD 776
            + +W +MSRNWG N+QSN+ L GQ+LSF+   SD
Sbjct:  196 SNKWETMSRNWGANYQSNTYLNGQSLSFKVQLSD 229

[Close]

BLAST of EY705312 vs. ExPASy Swiss-Prot
Match: EXP11_ORYSJ (Expansin-A11 OS=Oryza sativa subsp. japonica GN=EXPA11 PE=2 SV=2)

HSP 1 Score: 249.595 bits (636), Expect = 4.174e-73
Identity = 121/171 (70.76%), Postives = 132/171 (77.19%), Query Frame = 1

Query:  160 VFAGGP--WQSAHATFYGGSDASGTMGGACGYGNLYSQGYGVNTAALXTALFNNGLSCGACFELKCGGDPQWCNPGNPAILITATNFCPPNFAQPSDNGGWCNPPRPHFDLAMPMFLKLAQYRAGIVPVSYRRVPCRKRGGIRFTINGFRYFNLVLVTNVAGAGDIVRVSV 666
            V AGG   W S  ATFYGGSDASGTMGGACGYGNLYS GYG +TAAL TALFNNG SCGACFE++CGG    C  G  A+  TATN CPPN+A   D GGWCNPPRPHFD+A P F ++AQ RAG+VPV YRRV C K+GGIRFTI G  YFNLVLVTNV GAGD+  VSV
Sbjct:   19 VAAGGDSGWSSGSATFYGGSDASGTMGGACGYGNLYSAGYGTSTAALSTALFNNGQSCGACFEVRCGGGGS-CLAGTVAV--TATNLCPPNYALAGDAGGWCNPPRPHFDMAEPAFTRIAQARAGVVPVQYRRVACAKQGGIRFTITGHSYFNLVLVTNVGGAGDVTAVSV 186

HSP 2 Score: 47.3654 bits (111), Expect = 4.174e-73
Identity = 20/33 (60.61%), Postives = 23/33 (69.70%), Query Frame = 3

Query:  684 WLSMSRNWGQNWQSNSQLVGQALSFRSTGSDRR 782
            W +MS NWG NWQ+ + L GQ LSFR T SD R
Sbjct:  193 WQAMSHNWGANWQNGANLDGQPLSFRVTASDGR 225

The following BLAST results are available for this feature:

BLAST of EY705312 vs. ExPASy Swiss-Prot

BLAST of EY705312 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 88


Match Name	E-value	Identity	Description
EXPA2_ORYSJ	3.207e-82	73.94	Expansin-A2 OS=Oryza sativa subsp. japonica GN=EXP...	[more]
EXPA4_ORYSJ	7.118e-82	74.55	Expansin-A4 OS=Oryza sativa subsp. japonica GN=EXP...	[more]
EXPA4_ORYSI	7.118e-82	74.55	Expansin-A4 OS=Oryza sativa subsp. indica GN=EXPA4...	[more]
EXPA2_ARATH	3.504e-81	73.33	Expansin-A2 OS=Arabidopsis thaliana GN=EXPA2 PE=2 ...	[more]
EXPA8_ARATH	4.384e-80	73.33	Expansin-A8 OS=Arabidopsis thaliana GN=EXPA8 PE=2 ...	[more]
EXPA5_ORYSJ	1.884e-79	73.37	Expansin-A5 OS=Oryza sativa subsp. japonica GN=EXP...	[more]
EXPA6_ORYSJ	9.283e-79	70.48	Expansin-A6 OS=Oryza sativa subsp. japonica GN=EXP...	[more]
EXPA5_ARATH	1.443e-73	68.48	Expansin-A5 OS=Arabidopsis thaliana GN=EXPA5 PE=2 ...	[more]
EXP17_ARATH	4.173e-73	68.21	Putative expansin-A17 OS=Arabidopsis thaliana GN=E...	[more]
EXP11_ORYSJ	4.174e-73	70.76	Expansin-A11 OS=Oryza sativa subsp. japonica GN=EX...	[more]

Pages

Properties


Property Name	Value
Genbank description	CS00-C3-701-042-B09-CT.F Sweet orange fruit, development stadium (2 of 6) Citrus sinensis cDNA, mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>EY705312 ID=EY705312; Name=EY705312; organism=Citrus sinensis; type=EST; length=888bp
TCGCCCGACCCACGCGTCCGCTCCTGCGCGTTCACTCACTCACTCACTCA
ACTCTCTGTCTCCCTCTCTTCAACTCCAACAATGGCTCTTTTCAGAATGC
TCTGCTTTTTCTCACTGGCACTCTCCTTATTTGCGACGGCCAACGCCAAG
ATTCCCGGTGTCTTCGCCGGTGGCCCCTGGCAATCGGCCCACGCCACTTT
TTACGGTGGCAGCGATGCTTCCGGCACTATGGGTGGTGCTTGTGGGTACG
GGAATCTGTACAGCCAGGGCTACGGAGTGAACACGGCGGCTCTCANCACG
GCGCTGTTCAACAACGGCTTGAGCTGCGGCGCGTGCTTCGAGTTAAAGTG
CGGCGGCGACCCGCAGTGGTGTAACCCGGGTAACCCGGCCATTCTGATCA
CGGCCACCAACTTTTGCCCCCCGAACTTCGCTCAGCCCTCCGATAATGGC
GGGTGGTGCAACCCTCCTCGCCCTCATTTCGATCTGGCCATGCCTATGTT
CCTCAAGCTTGCCCAGTACCGCGCTGGTATCGTCCCCGTTTCTTACCGCA
GGGTGCCCTGCAGAAAGCGAGGCGGAATCAGATTCACAATTAACGGTTTC
CGTTACTTCAACTTGGTCTTGGTCACCAACGTCGCGGGCGCAGGGGATAT
CGTGCGCGTGAGCGTGAAAGGGGCAACACCCAATGGCTGAGCATGAGCCG
CAACTGGGGGCAAAACTGGCAATCCAACTCTCAGCTCGTGGGCCAGGCAC
TTTCGTTTAGAAGTACGGGCAGTGACAGAAGACCCTCACCTCTTNGGACG
TGGCGCCAGCGAATTNGGCAGTCNGTCAACCTTTTTCGGAAAGATTTCCG
CGTCTAGTGAATTTTATTTTCTTTTTCACATTTTCATG