EY705311 | Citrus Genome Database

Overview


Name	EY705311
Unique Name	EY705311
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	944

Libraries


Library Name	Type
Sweet orange fruit, development stadium (2 of 6)	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Alignments

Feature Name	Type	Location	Analysis
Csv1_Contig6249	contig	Csv1_Contig6249:1..905.	BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1

Homology

[Close]

BLAST of EY705311 vs. ExPASy Swiss-Prot
Match: RBS6_FLAPR (Ribulose bisphosphate carboxylase small chain 6, chloroplastic OS=Flaveria pringlei GN=RBCS6 PE=2 SV=1)

HSP 1 Score: 208.379 bits (529), Expect = 3.100e-55
Identity = 93/139 (66.91%), Postives = 119/139 (85.61%), Query Frame = 1

Query:  139 ANRASLAQASMVAPFTGLKSSSAFPATKKTNNDITSIASNGGRVQCMKVWPPTGLKKFETLSYLPPLSDEALLKEINYLIRSGWIPCLEFELEKGWVYREHHKSPGYYDGRYWTMWKLPMYGCTDATQVLKEVGEVQKD 555
            A  A++AQA+MVAPFTGLK+++AFP TKK N D +++ SNGGRVQCMKVWPP G KK+ TLSYLP L++  L KE++YL+R+ W+PCLEFELE G+VYRE+ +SPGYYDGRYWTMWKLPM+GCTD+ QV+KE+ E +K+
Sbjct:    6 ATVAAVAQANMVAPFTGLKANAAFPVTKKVN-DFSTLPSNGGRVQCMKVWPPLGKKKYXTLSYLPNLTEAQLAKEVDYLLRNKWVPCLEFELEHGFVYRENARSPGYYDGRYWTMWKLPMFGCTDSVQVMKELQECKKE 143

HSP 2 Score: 28.8758 bits (63), Expect = 3.100e-55
Identity = 12/13 (92.31%), Postives = 13/13 (100.00%), Query Frame = 2

Query:  596 RQVQCISFIAAKP 634
            RQVQCISFIA+KP
Sbjct:  158 RQVQCISFIASKP 170

[Close]

BLAST of EY705311 vs. ExPASy Swiss-Prot
Match: RBS_FLATR (Ribulose bisphosphate carboxylase small chain, chloroplastic OS=Flaveria trinervia GN=RBCS PE=2 SV=1)

HSP 1 Score: 204.912 bits (520), Expect = 1.167e-54
Identity = 91/139 (65.47%), Postives = 116/139 (83.45%), Query Frame = 1

Query:  139 ANRASLAQASMVAPFTGLKSSSAFPATKKTNNDITSIASNGGRVQCMKVWPPTGLKKFETLSYLPPLSDEALLKEINYLIRSGWIPCLEFELEKGWVYREHHKSPGYYDGRYWTMWKLPMYGCTDATQVLKEVGEVQKD 555
            A  A++ Q +MVAPFTGLK+++AFP TKK N   +++ SNGGRVQCMKVWPP G KK+ETLSYLP L++  L KE++YL+R+ W+PCLEFELE G+VYRE+  SPGYYDGRYWTMWKLPM+GCTD+ QV+KE+ E +K+
Sbjct:    6 ATVAAVPQTNMVAPFTGLKANAAFPVTKKVNG-FSTLPSNGGRVQCMKVWPPVGKKKYETLSYLPELTEAQLAKEVDYLLRNKWVPCLEFELEHGFVYRENASSPGYYDGRYWTMWKLPMFGCTDSAQVMKELQECKKE 143

HSP 2 Score: 30.4166 bits (67), Expect = 1.167e-54
Identity = 12/15 (80.00%), Postives = 14/15 (93.33%), Query Frame = 2

Query:  596 RQVQCISFIAAKPPG 640
            RQVQC+SFIA+KP G
Sbjct:  158 RQVQCVSFIASKPTG 172

[Close]

BLAST of EY705311 vs. ExPASy Swiss-Prot
Match: RBS1_LEMGI (Ribulose bisphosphate carboxylase small chain SSU1, chloroplastic OS=Lemna gibba GN=SSU1 PE=2 SV=2)

HSP 1 Score: 204.527 bits (519), Expect = 1.984e-54
Identity = 95/147 (64.63%), Postives = 119/147 (80.95%), Query Frame = 1

Query:  112 MISSATVATANRASLAQASMVAPFTGLKSSSAFPATKKTNNDITSIASNGGRVQCMKVWPPTGLKKFETLSYLPPLSDEALLKEINYLIRSGWIPCLEFELEKGWVYREHHKSPGYYDGRYWTMWKLPMYGCTDATQVLKEVGEVQK 552
            M+S+A VA   R   AQ +MV  F G +SS AFPAT+K NND++++ S+GGRV CM+VWPP GLKKFETLSYLPPLS E L KE++YL+R+ W+PC+EF  E G+VYRE++ SPGYYDGRYWTMWKLPM+GCTDA+QV+ EV E +K
Sbjct:    2 MVSTAAVA---RVRPAQTNMVGAFNGCRSSVAFPATRKANNDLSTLPSSGGRVSCMQVWPPEGLKKFETLSYLPPLSVEDLAKEVDYLLRNDWVPCIEFSKE-GFVYRENNASPGYYDGRYWTMWKLPMFGCTDASQVIAEVEEAKK 144

HSP 2 Score: 30.0314 bits (66), Expect = 1.984e-54
Identity = 13/15 (86.67%), Postives = 14/15 (93.33%), Query Frame = 2

Query:  590 HKRQVQCISFIAAKP 634
            +KRQVQCISFIA KP
Sbjct:  158 NKRQVQCISFIAYKP 172

[Close]

BLAST of EY705311 vs. ExPASy Swiss-Prot
Match: RBS_ZANAE (Ribulose bisphosphate carboxylase small chain, chloroplastic OS=Zantedeschia aethiopica GN=RBCS PE=2 SV=1)

HSP 1 Score: 199.904 bits (507), Expect = 3.055e-52
Identity = 98/151 (64.90%), Postives = 120/151 (79.47%), Query Frame = 1

Query:  100 MASSMISSATVATANRASLAQASMVAPFTGLKSSSAFPATKKTNNDITSIASNGGRVQCMKVWPPTGLKKFETLSYLPPLSDEALLKEINYLIRSGWIPCLEFELEKGWVYREHHKSPGYYDGRYWTMWKLPMYGCTDATQVLKEVGEVQK 552
            MASSM+ S   A  +R S AQ++MV PF GL SS+AFP T+K   D + + SNG RV+CM+VWPP G KKFETLSYLPPL+ E L +E++YLIR+GWIPC+EF  + G+VYRE+H SPGYYDGRYWTMWKLPM+GCTD++QVLKEV E  K
Sbjct:    1 MASSMMVST--AAVSRTSPAQSNMVVPFAGLHSSAAFPVTRKFA-DSSKLPSNGLRVRCMQVWPPEGKKKFETLSYLPPLTREQLGQEVDYLIRNGWIPCIEF-CKVGFVYREYHNSPGYYDGRYWTMWKLPMFGCTDSSQVLKEVDECSK 147

HSP 2 Score: 27.335 bits (59), Expect = 3.055e-52
Identity = 11/15 (73.33%), Postives = 12/15 (80.00%), Query Frame = 2

Query:  596 RQVQCISFIAAKPPG 640
            RQVQCISF+  KP G
Sbjct:  163 RQVQCISFLTYKPEG 177

[Close]

BLAST of EY705311 vs. ExPASy Swiss-Prot
Match: RBS_HORVU (Ribulose bisphosphate carboxylase small chain, chloroplastic OS=Hordeum vulgare PE=2 SV=1)

HSP 1 Score: 193.356 bits (490), Expect = 8.827e-52
Identity = 87/133 (65.41%), Postives = 112/133 (84.21%), Query Frame = 1

Query:  157 AQASMVAPFTGLKSSSAFPATKKTNNDITSIASNGGRVQCMKVWPPTGLKKFETLSYLPPLSDEALLKEINYLIRSGWIPCLEFELEKGWVYREHHKSPGYYDGRYWTMWKLPMYGCTDATQVLKEVGEVQKD 555
            + A+ VAPF GLKS++  P ++++N    S+ SNGGR++CM+VWP  G+KKFETLSYLPPLS EALLK+++YLIRS W+PCLEF  + G+++REH+ SPGYYDGRYWTMWKLPM+GCTDATQVL EV EV+K+
Sbjct:    8 SSATSVAPFQGLKSTAGLPVSRRSNASSASV-SNGGRIRCMQVWPIEGIKKFETLSYLPPLSTEALLKQVDYLIRSKWVPCLEFS-KVGFIFREHNASPGYYDGRYWTMWKLPMFGCTDATQVLNEVEEVKKE 138

HSP 2 Score: 32.3426 bits (72), Expect = 8.827e-52
Identity = 13/15 (86.67%), Postives = 14/15 (93.33%), Query Frame = 2

Query:  596 RQVQCISFIAAKPPG 640
            RQVQC+SFIA KPPG
Sbjct:  153 RQVQCVSFIAFKPPG 167

[Close]

BLAST of EY705311 vs. ExPASy Swiss-Prot
Match: RBS1_ORYSJ (Ribulose bisphosphate carboxylase small chain, chloroplastic OS=Oryza sativa subsp. japonica GN=RBCS PE=1 SV=1)

HSP 1 Score: 190.274 bits (482), Expect = 2.640e-51
Identity = 85/132 (64.39%), Postives = 107/132 (81.06%), Query Frame = 1

Query:  157 AQASMVAPFTGLKSSSAFPATKKTNNDITSIASNGGRVQCMKVWPPTGLKKFETLSYLPPLSDEALLKEINYLIRSGWIPCLEFELEKGWVYREHHKSPGYYDGRYWTMWKLPMYGCTDATQVLKEVGEVQK 552
            + A+ VAPF GLKS++  P  +++ N      SNGGR++CM+VWP  G+KKFETLSYLPPL+ E LLK+I YL+RS W+PCLEF  + G+VYRE+H+SPGYYDGRYWTMWKLPM+GCTDATQVLKE+ E +K
Sbjct:    8 SSATTVAPFQGLKSTAGMPVARRSGNSSFGNVSNGGRIRCMQVWPIEGIKKFETLSYLPPLTVEDLLKQIEYLLRSKWVPCLEFS-KVGFVYRENHRSPGYYDGRYWTMWKLPMFGCTDATQVLKELEEAKK 138

HSP 2 Score: 28.8758 bits (63), Expect = 2.640e-51
Identity = 13/15 (86.67%), Postives = 13/15 (86.67%), Query Frame = 2

Query:  596 RQVQCISFIAAKPPG 640
            RQVQ ISFIA KPPG
Sbjct:  154 RQVQLISFIAYKPPG 168

HSP 3 Score: 25.7942 bits (55), Expect = 2.640e-51
Identity = 10/12 (83.33%), Postives = 11/12 (91.67%), Query Frame = 3

Query:  555 YPHSFVRIIGFD 590
            YP +FVRIIGFD
Sbjct:  140 YPDAFVRIIGFD 151

[Close]

BLAST of EY705311 vs. ExPASy Swiss-Prot
Match: RBS1_ORYSI (Ribulose bisphosphate carboxylase small chain, chloroplastic OS=Oryza sativa subsp. indica GN=RBCS PE=2 SV=2)

HSP 1 Score: 190.274 bits (482), Expect = 2.640e-51
Identity = 85/132 (64.39%), Postives = 107/132 (81.06%), Query Frame = 1

Query:  157 AQASMVAPFTGLKSSSAFPATKKTNNDITSIASNGGRVQCMKVWPPTGLKKFETLSYLPPLSDEALLKEINYLIRSGWIPCLEFELEKGWVYREHHKSPGYYDGRYWTMWKLPMYGCTDATQVLKEVGEVQK 552
            + A+ VAPF GLKS++  P  +++ N      SNGGR++CM+VWP  G+KKFETLSYLPPL+ E LLK+I YL+RS W+PCLEF  + G+VYRE+H+SPGYYDGRYWTMWKLPM+GCTDATQVLKE+ E +K
Sbjct:    8 SSATTVAPFQGLKSTAGMPVARRSGNSSFGNVSNGGRIRCMQVWPIEGIKKFETLSYLPPLTVEDLLKQIEYLLRSKWVPCLEFS-KVGFVYRENHRSPGYYDGRYWTMWKLPMFGCTDATQVLKELEEAKK 138

HSP 2 Score: 28.8758 bits (63), Expect = 2.640e-51
Identity = 13/15 (86.67%), Postives = 13/15 (86.67%), Query Frame = 2

Query:  596 RQVQCISFIAAKPPG 640
            RQVQ ISFIA KPPG
Sbjct:  154 RQVQLISFIAYKPPG 168

HSP 3 Score: 25.7942 bits (55), Expect = 2.640e-51
Identity = 10/12 (83.33%), Postives = 11/12 (91.67%), Query Frame = 3

Query:  555 YPHSFVRIIGFD 590
            YP +FVRIIGFD
Sbjct:  140 YPDAFVRIIGFD 151

[Close]

BLAST of EY705311 vs. ExPASy Swiss-Prot
Match: RBS2_ORYSJ (Ribulose bisphosphate carboxylase small chain A, chloroplastic OS=Oryza sativa subsp. japonica GN=RBCS-A PE=2 SV=2)

HSP 1 Score: 189.889 bits (481), Expect = 3.436e-51
Identity = 85/132 (64.39%), Postives = 108/132 (81.82%), Query Frame = 1

Query:  157 AQASMVAPFTGLKSSSAFPATKKTNNDITSIASNGGRVQCMKVWPPTGLKKFETLSYLPPLSDEALLKEINYLIRSGWIPCLEFELEKGWVYREHHKSPGYYDGRYWTMWKLPMYGCTDATQVLKEVGEVQK 552
            + A+ VAPF GLKS++  P ++++ N      SNGGR++CM+VWP  G+KKFETLSYLPPL+ E LLK+I YL+RS W+PCLEF  + G+VYRE+H+SPGYYDGRYWTMWKLPM+GCTDATQVLKE+ E +K
Sbjct:    8 SSATSVAPFQGLKSTAGLPVSRRSTNSGFGNVSNGGRIKCMQVWPIEGIKKFETLSYLPPLTVEDLLKQIEYLLRSKWVPCLEFS-KVGFVYRENHRSPGYYDGRYWTMWKLPMFGCTDATQVLKELEEAKK 138

HSP 2 Score: 28.8758 bits (63), Expect = 3.436e-51
Identity = 13/15 (86.67%), Postives = 13/15 (86.67%), Query Frame = 2

Query:  596 RQVQCISFIAAKPPG 640
            RQVQ ISFIA KPPG
Sbjct:  154 RQVQLISFIAYKPPG 168

HSP 3 Score: 25.7942 bits (55), Expect = 3.436e-51
Identity = 10/12 (83.33%), Postives = 11/12 (91.67%), Query Frame = 3

Query:  555 YPHSFVRIIGFD 590
            YP +FVRIIGFD
Sbjct:  140 YPDAFVRIIGFD 151

[Close]

BLAST of EY705311 vs. ExPASy Swiss-Prot
Match: RBS2_WHEAT (Ribulose bisphosphate carboxylase small chain PW9, chloroplastic OS=Triticum aestivum PE=3 SV=1)

HSP 1 Score: 191.815 bits (486), Expect = 5.643e-51
Identity = 87/133 (65.41%), Postives = 110/133 (82.71%), Query Frame = 1

Query:  157 AQASMVAPFTGLKSSSAFPATKKTNNDITSIASNGGRVQCMKVWPPTGLKKFETLSYLPPLSDEALLKEINYLIRSGWIPCLEFELEKGWVYREHHKSPGYYDGRYWTMWKLPMYGCTDATQVLKEVGEVQKD 555
            + A+ VAPF GLKS++  P + ++ +   S  SNGGR++CM+VWP  G+KKFETLSYLPPLS EALLK+++YLIRS W+PCLEF  + G+V+REH+ SPGYYDGRYWTMWKLPM+GCTDATQVL EV EV+K+
Sbjct:    8 SSATTVAPFQGLKSTAGLPISCRSGSTGLSSVSNGGRIRCMQVWPIEGIKKFETLSYLPPLSTEALLKQVDYLIRSKWVPCLEFS-KVGFVFREHNSSPGYYDGRYWTMWKLPMFGCTDATQVLNEVEEVKKE 139

HSP 2 Score: 31.187 bits (69), Expect = 5.643e-51
Identity = 12/15 (80.00%), Postives = 14/15 (93.33%), Query Frame = 2

Query:  596 RQVQCISFIAAKPPG 640
            RQVQC+SFIA +PPG
Sbjct:  154 RQVQCVSFIAFRPPG 168

[Close]

BLAST of EY705311 vs. ExPASy Swiss-Prot
Match: RBS1_WHEAT (Ribulose bisphosphate carboxylase small chain PWS4.3, chloroplastic OS=Triticum aestivum PE=3 SV=2)

HSP 1 Score: 191.045 bits (484), Expect = 9.592e-51
Identity = 86/133 (64.66%), Postives = 111/133 (83.46%), Query Frame = 1

Query:  157 AQASMVAPFTGLKSSSAFPATKKTNNDITSIASNGGRVQCMKVWPPTGLKKFETLSYLPPLSDEALLKEINYLIRSGWIPCLEFELEKGWVYREHHKSPGYYDGRYWTMWKLPMYGCTDATQVLKEVGEVQKD 555
            + A+ VAPF GLKS++  P ++++   + S+ SNGGR++CM+VWP  G+KKFETLSYLPPLS EAL K+++YLIRS W+PCLEF  + G+V+REH+ SPGYYDGRYWTMWKLPM+GCTDATQVL EV EV+K+
Sbjct:    8 SSATTVAPFQGLKSTAGLPVSRRSRGSLGSV-SNGGRIRCMQVWPIEGIKKFETLSYLPPLSTEALSKQVDYLIRSKWVPCLEFS-KVGFVFREHNSSPGYYDGRYWTMWKLPMFGCTDATQVLNEVEEVKKE 138

HSP 2 Score: 31.187 bits (69), Expect = 9.592e-51
Identity = 12/15 (80.00%), Postives = 14/15 (93.33%), Query Frame = 2

Query:  596 RQVQCISFIAAKPPG 640
            RQVQC+SFIA +PPG
Sbjct:  153 RQVQCVSFIAFRPPG 167

The following BLAST results are available for this feature:

BLAST of EY705311 vs. ExPASy Swiss-Prot

BLAST of EY705311 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 112


Match Name	E-value	Identity	Description
RBS6_FLAPR	3.100e-55	66.91	Ribulose bisphosphate carboxylase small chain 6, c...	[more]
RBS_FLATR	1.167e-54	65.47	Ribulose bisphosphate carboxylase small chain, chl...	[more]
RBS1_LEMGI	1.984e-54	64.63	Ribulose bisphosphate carboxylase small chain SSU1...	[more]
RBS_ZANAE	3.055e-52	64.90	Ribulose bisphosphate carboxylase small chain, chl...	[more]
RBS_HORVU	8.827e-52	65.41	Ribulose bisphosphate carboxylase small chain, chl...	[more]
RBS1_ORYSJ	2.640e-51	64.39	Ribulose bisphosphate carboxylase small chain, chl...	[more]
RBS1_ORYSI	2.640e-51	64.39	Ribulose bisphosphate carboxylase small chain, chl...	[more]
RBS2_ORYSJ	3.436e-51	64.39	Ribulose bisphosphate carboxylase small chain A, c...	[more]
RBS2_WHEAT	5.643e-51	65.41	Ribulose bisphosphate carboxylase small chain PW9,...	[more]
RBS1_WHEAT	9.592e-51	64.66	Ribulose bisphosphate carboxylase small chain PWS4...	[more]

Pages

Properties


Property Name	Value
Genbank description	CS00-C3-701-042-B08-CT.F Sweet orange fruit, development stadium (2 of 6) Citrus sinensis cDNA, mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>EY705311 ID=EY705311; Name=EY705311; organism=Citrus sinensis; type=EST; length=944bp
TTTCCGACCCACGCGTCCGATTATATAAACCTACCGCCCACTGAGCATCC
TCCTATTAAGTAAAATAACTTGCAAAGCTAGAAGAAGGATCAAGTACCAA
TGGCTTCCTCAATGATCTCATCGGCCACCGTTGCCACCGCGAACCGCGCC
AGCCTTGCTCAGGCCAGCATGGTCGCCCCCTTCACCGGCCTCAAGTCTTC
TTCTGCTTTCCCAGCAACCAAGAAGACCAACAATGACATTACTTCCATTG
CAAGCAACGGCGGAAGAGTCCAATGCATGAAGGTGTGGCCACCAACTGGC
TTGAAGAAGTTCGAGACTCTCTCATACCTCCCGCCACTGTCCGATGAAGC
TTTGCTCAAGGAAATCAATTACCTTATCCGCTCCGGCTGGATTCCCTGCT
TGGAATTCGAGTTGGAGAAAGGATGGGTGTACCGTGAGCACCACAAGTCA
CCAGGATACTACGATGGACGCTACTGGACAATGTGGAAGCTGCCCATGTA
TGGGTGCACAGATGCAACCCAGGTTTTGAAGGAGGTTGGGGAGGTCCAGA
AGGATACCCACATTCATTCGTCCGAATTATCGGATTTGACACAAGCGTCA
AGTGCAGTGCATCAGTTTCATTGCTGCCAAGCCACCAGGCGTCTAGGTTC
CCAATTAAATTTGTGNTACCACCCATGCAATATTGTTGATTATTATTATG
TGTAACCCCCATAATGATCATTCGATCAATGGGGGTTCGGTTTGCGTTTA
ATTTGTACTTANGCTGCTGTTGCTGCTGCTCAAACTTTTTTGGTTTTCTC
CCGACNNTTGCTNCTTTTTTTTTTTTTTAATATATTTAATAATNGAATCC
TTTGNTTCAGNTNCATAATCTGGGTCTCGGGATGTCAGTTAATGAGTGAG
AATACTGGAATTAAAGAGTAGTTTATGATTCTTGCAAAAAAAAA