EY659305

Overview
NameEY659305
Unique NameEY659305
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length883
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
BLAST of EY659305 vs. ExPASy Swiss-Prot
Match: SCP45_ARATH (Serine carboxypeptidase-like 45 OS=Arabidopsis thaliana GN=SCPL45 PE=2 SV=1)

HSP 1 Score: 137.117 bits (344), Expect = 1.271e-31
Identity = 76/200 (38.00%), Postives = 106/200 (53.00%), Query Frame = 1
Query:  109 LDMAAMEQLGPFIVNSDG*TLYRNEYAWNNVANVLFLETPSRVRFSYSNTSSDYRNPGDNNTSDDSYTFLTNWFERFPQYKNRDFFITGEIYAGHYVPQLAHTIVSKNTSKTIINLKCISIGNAWIDDNLCTKRMLDSFWTHALNSYETNAEINKYCDYA-------TGQLSTSCDQYQT-L*VMAVRQIDLYNVYAPLC 684
            L + A  + GPF     G  L +N+++WN  AN+L+LETP  V FSYS  SS Y    D  T+ D+  FL  WF +FP Y NR  FITGE YAGHYVPQLA  ++  N    + NL+ I+IGN  ++         + FW+H L S  T      YC+Y+        G +S+ C +  + +     R +D Y+V   +C
Sbjct:   89 LGVGAFSENGPF--RPKGPILVKNQHSWNQEANMLYLETPVGVGFSYSTQSSHYEGVNDKITARDNLVFLQRWFLKFPHYLNRSLFITGESYAGHYVPQLAELMIQYNKKHHLFNLRGIAIGNPVLEFATDFNSRAEYFWSHGLISDSTYKMFTSYCNYSRYVSEYYRGSMSSMCSKVMSQVSTETSRFVDKYDVTLDVC 286          
BLAST of EY659305 vs. ExPASy Swiss-Prot
Match: NF314_NAEFO (Virulence-related protein Nf314 OS=Naegleria fowleri PE=2 SV=1)

HSP 1 Score: 111.309 bits (277), Expect = 2.746e-31
Identity = 65/183 (35.52%), Postives = 98/183 (53.55%), Query Frame = 1
Query:  136 GPFIVNSDG*TLYRNEYAWNNVANVLFLETPSRVRFSYSNTSSDYRNPGDNNTSDDSYTFLTNWFERFPQYKNRDFFITGEIYAGHYVPQLAHTIVSKN--TSKTIINLKCISIGNAWIDDNLCTKRMLDSFWTHALNSYETNAEINKYC--DYATGQLSTSCDQYQTL*VMAVRQIDLYNVY 672
            G F+VN+DG T+ RN Y+WN V+N+L++E P  V FSYSN++ DY+N  D   + D    L ++  RFPQ+  R+ ++ GE Y G YVP  A+ IV  N    +  +NL  I +GN   D    +  +      H+L S +   E  K C  D+   Q   +C ++ T    A+  I+ Y +Y
Sbjct:   82 GLFLVNADGATITRNPYSWNRVSNILYIEQPVGVGFSYSNSTDDYQNLNDVQAASDMNNALRDFLTRFPQFIGRETYLAGESYGGVYVPTTAYNIVEGNGKGQQPYVNLVGILVGNGVTDAEADSNSIPPMMKYHSLISIKYYEEGYKACQGDFYANQNLPACQKFLTDSSNAMGNINPYYIY 264          

HSP 2 Score: 45.0542 bits (105), Expect = 2.746e-31
Identity = 19/31 (61.29%), Postives = 23/31 (74.19%), Query Frame = 3
Query:   21 LFYYFAESPHSSSTKPMVLWLNGGP*CSSLG 113
            LFY+F ES  + S  P+V+W NGGP CSSLG
Sbjct:   45 LFYWFFESMRNPSQDPLVMWTNGGPGCSSLG 75          
BLAST of EY659305 vs. ExPASy Swiss-Prot
Match: SCP20_ARATH (Serine carboxypeptidase-like 20 OS=Arabidopsis thaliana GN=SCPL20 PE=2 SV=2)

HSP 1 Score: 106.301 bits (264), Expect = 1.743e-30
Identity = 61/173 (35.26%), Postives = 92/173 (53.18%), Query Frame = 1
Query:  169 LYRNEYAWNNVANVLFLETPSRVRFSYSNTSSDYRNPGDNNTSDDSYTFLTNWFERFPQYKNRDFFITGEIYAGHYVPQLAHTIV--SKNTSKTIINLKCISIGNAWIDDNLCTKRMLDSFWTHALNSYETNAEINKYCDYATGQL-STSCDQYQTL*VMAVRQIDLYNVYAP 678
            L+ N Y+W+ V+N+++L++P  V FSYSN  SDY   GD  T+ DS+ FL  WF+ FP++++  FFI+GE YAG YVP LA  +V  +KN  K  +N K   +GN   D        +       L S E    + K C     ++    C++  T       Q+++YN+  P
Sbjct:  117 LHLNPYSWSKVSNIIYLDSPVGVGFSYSNNKSDYIT-GDIKTAVDSHAFLLKWFQMFPEFQSNPFFISGESYAGVYVPTLASEVVIGNKNGVKPALNFKGYLVGNGVADPKFDGNAFVPFAHGMGLISDELFENVTKACKGNFYEIEGLECEEQYTKVNDDTNQLNIYNILEP 288          

HSP 2 Score: 47.3654 bits (111), Expect = 1.743e-30
Identity = 24/44 (54.55%), Postives = 29/44 (65.91%), Query Frame = 3
Query:   15 KTLFYYFAESPHSSSTKPMVLWLNGGP*CSSLGYGCHGTTGPFH 146
            K L+YYF ES  + S  P+VLWLNGGP CSS+  G     GPF+
Sbjct:   62 KNLWYYFIESEKNPSKDPVVLWLNGGPGCSSMD-GFVYEHGPFN 104          
BLAST of EY659305 vs. ExPASy Swiss-Prot
Match: SCP53_ARATH (Putative serine carboxypeptidase-like 53 OS=Arabidopsis thaliana GN=SCPL53 PE=5 SV=1)

HSP 1 Score: 132.88 bits (333), Expect = 2.398e-30
Identity = 58/93 (62.37%), Postives = 72/93 (77.42%), Query Frame = 1
Query:  121 AMEQLGPFIVNSDG*TLYRNEYAWNNVANVLFLETPSRVRFSYSNTSSDYRNPGDNNTSDDSYTFLTNWFERFPQYKNRDFFITGEIYAGHYV 399
            A ++LGPF V+SD  TLY N Y+WNNVAN+LFLE+P+   FSY+NT++D  NPGD  T+ D+Y FL  W ERFP+YK RDF+I GE YAGHYV
Sbjct:  140 AFQELGPFRVHSDNKTLYTNPYSWNNVANMLFLESPAGTGFSYTNTTTDMENPGDMKTAADNYVFLVKWLERFPEYKGRDFYIAGESYAGHYV 232          
BLAST of EY659305 vs. ExPASy Swiss-Prot
Match: CBP21_HORVU (Serine carboxypeptidase II-1 (Fragment) OS=Hordeum vulgare GN=CXP;2-1 PE=1 SV=1)

HSP 1 Score: 129.028 bits (323), Expect = 3.462e-29
Identity = 77/212 (36.32%), Postives = 107/212 (50.47%), Query Frame = 1
Query:  262 SDYRNPGDNNTSDDSYTFLTNWFERFPQYKNRDFFITGEIYAGHYVPQLAHTI--VSKNTSKTIINLKCISIGNAWIDDNLCTKRMLDSFWTHALNSYETNAEINKYCDYATGQ-LSTSCDQYQTL*VMAVRQIDLYNVYAPLCKSSAPPPPTAL------VIREYDPCSETSFIPIKRLPSASSSXCDT-----YNWSTRXD*T---WTDS 846
            SD   PGDN T+ DSY FL NW ERFPQYK RDF+I GE YAGHYVPQL+  +   +K   K I+N K   +GNA IDD        + +WTH L S +T  ++   C++ + +  S +C++   +       ID Y++Y P CK ++      +      + R YDPC+E        LP    +         Y+W+   D     W DS
Sbjct:    2 SDLLTPGDNKTAHDSYAFLVNWLERFPQYKYRDFYIAGESYAGHYVPQLSQLVHRNNKGVRKPILNFKGFMVGNAVIDDYHDFVGTFEYWWTHGLISDDTYQKLQLACEFDSAEHESEACNKINNVAEAEEGLIDAYSIYTPTCKKTSLHRRRLIKGRRPWLPRGYDPCTEQYSTKYYNLPEVQKAFRANVTGIPYSWTACSDVLSDHWKDS 213          
BLAST of EY659305 vs. ExPASy Swiss-Prot
Match: PPGB_BOVIN (Lysosomal protective protein OS=Bos taurus GN=CTSA PE=2 SV=1)

HSP 1 Score: 101.679 bits (252), Expect = 1.548e-28
Identity = 68/202 (33.66%), Postives = 104/202 (51.49%), Query Frame = 1
Query:  136 GPFIVNSDG*TLYRNEYAWNNVANVLFLETPSRVRFSYSNTSSDYRNPGDNNTSDDSYTFLTNWFERFPQYKNRDFFITGEIYAGHYVPQLAHTIVSKNTSKTIINLKCISIGNAWI----DDN----------LCTKRMLDSFWTHALNSYETNAEINKYCDYATGQLSTSCDQYQTL*VMAVRQIDLYNVYAPLCKSSAP 699
            GPF++  DG TL  N Y+WN +ANVL+LE+P+ V FSYS+  S   N  D   +  ++  L ++F  FP+YK  + F+TGE YAG Y+P LA  +V ++ S   +NL+ +++GN       +DN          L   R+  S  TH  +  + N   NK  +        S        ++A   +++YN+YAP C    P
Sbjct:   98 GPFLIQPDGVTLEYNPYSWNLIANVLYLESPAGVGFSYSDDKSYATN--DTEVAQSNFEALKDFFCLFPEYKGNELFLTGESYAGIYIPTLA-VLVMQDPS---MNLQGLAVGNGLSSYEQNDNSLVYFAYYHGLLGNRLWSSLQTHCCSQNQCNFHDNKEPECVANLQEVSH-------IVASSGLNIYNLYAP-CAGGVP 285          

HSP 2 Score: 45.4394 bits (106), Expect = 1.548e-28
Identity = 23/44 (52.27%), Postives = 27/44 (61.36%), Query Frame = 3
Query:   12 AKTLFYYFAESPHSSSTKPMVLWLNGGP*CSSLGYGCHGTTGPF 143
            +K L Y+F ES     + P+VLWLNGGP CSSL  G     GPF
Sbjct:   58 SKRLHYWFVESQKDPKSSPVVLWLNGGPGCSSLD-GLLTEHGPF 100          
BLAST of EY659305 vs. ExPASy Swiss-Prot
Match: SCPL1_DICDI (Serine carboxypeptidase S10 family member 1 OS=Dictyostelium discoideum GN=DDB_G0291912 PE=3 SV=1)

HSP 1 Score: 99.7525 bits (247), Expect = 2.630e-28
Identity = 52/125 (41.60%), Postives = 73/125 (58.40%), Query Frame = 1
Query:  115 MAAMEQLGPFIVNSDG*TLYRNEYAWNNVANVLFLETPSRVRFSYSNTSSDYRNPGDNNTSDDSYTFLTNWFERFPQYKNRDFFITGEIYAGHYVPQLAHTIVSKNTSKTIINLKCISIGNAWID 489
            MAA  + GP+ VN D  TL  N  +WN VANVL++++P    FSY   S  Y    +   S++ Y+FLT +  ++P+Y     +I GE YAGHYVP  ++ I  KN     INLK ++IGN  +D
Sbjct:   70 MAAFYENGPYFVN-DNLTLSENPNSWNMVANVLYVDSPLGAGFSYVVDSDGYSTT-ETEISENLYSFLTQFLSKYPKYSKLPLYIFGESYAGHYVPSFSYYIYQKNLGLATINLKGLAIGNGMVD 192          

HSP 2 Score: 46.595 bits (109), Expect = 2.630e-28
Identity = 21/34 (61.76%), Postives = 24/34 (70.59%), Query Frame = 3
Query:    9 TAKTLFYYFAESPHSSSTKPMVLWLNGGP*CSSL 110
            T   LFY F ES +S ST P++LWL GGP CSSL
Sbjct:   36 TNANLFYLFYESQNSPSTDPLILWLTGGPGCSSL 69          
BLAST of EY659305 vs. ExPASy Swiss-Prot
Match: PPGB_MOUSE (Lysosomal protective protein OS=Mus musculus GN=Ctsa PE=1 SV=1)

HSP 1 Score: 102.064 bits (253), Expect = 3.417e-28
Identity = 68/202 (33.66%), Postives = 104/202 (51.49%), Query Frame = 1
Query:  136 GPFIVNSDG*TLYRNEYAWNNVANVLFLETPSRVRFSYSNTSSDYRNPGDNNTSDDSYTFLTNWFERFPQYKNRDFFITGEIYAGHYVPQLAHTIVSKNTSKTIINLKCISIGNAWI----DDN----------LCTKRMLDSFWTHALNSYETNAEINKYCDYATGQLSTSCDQYQTL*VMAVRQIDLYNVYAPLCKSSAP 699
            GPF++  DG TL  N YAWN +ANVL++E+P+ V FSYS+      N  D   ++++Y  L ++F  FP+YK+   F+TGE YAG Y+P LA  +V ++ S   +NL+ +++GN       +DN          L   R+  S  TH     + N   NK  +     L  S        ++    +++YN+YAP C    P
Sbjct:   94 GPFLIQPDGVTLEYNPYAWNLIANVLYIESPAGVGFSYSDDKMYVTN--DTEVAENNYEALKDFFRLFPEYKDNKLFLTGESYAGIYIPTLA-VLVMQDPS---MNLQGLAVGNGLASYEQNDNSLVYFAYYHGLLGNRLWTSLQTHCCAQNKCNFYDNKDPECVNNLLEVSR-------IVGKSGLNIYNLYAP-CAGGVP 281          

HSP 2 Score: 43.8986 bits (102), Expect = 3.417e-28
Identity = 22/44 (50.00%), Postives = 26/44 (59.09%), Query Frame = 3
Query:   12 AKTLFYYFAESPHSSSTKPMVLWLNGGP*CSSLGYGCHGTTGPF 143
            +K   Y+F ES +     P+VLWLNGGP CSSL  G     GPF
Sbjct:   54 SKHFHYWFVESQNDPKNSPVVLWLNGGPGCSSLD-GLLTEHGPF 96          
BLAST of EY659305 vs. ExPASy Swiss-Prot
Match: PPGB_HUMAN (Lysosomal protective protein OS=Homo sapiens GN=CTSA PE=1 SV=2)

HSP 1 Score: 100.908 bits (250), Expect = 4.447e-28
Identity = 67/202 (33.17%), Postives = 104/202 (51.49%), Query Frame = 1
Query:  136 GPFIVNSDG*TLYRNEYAWNNVANVLFLETPSRVRFSYSNTSSDYRNPGDNNTSDDSYTFLTNWFERFPQYKNRDFFITGEIYAGHYVPQLAHTIVSKNTSKTIINLKCISIGNAWI----DDN----------LCTKRMLDSFWTHALNSYETNAEINKYCDYATGQLSTSCDQYQTL*VMAVRQIDLYNVYAPLCKSSAP 699
            GPF+V  DG TL  N Y+WN +ANVL+LE+P+ V FSYS+    +    D   +  ++  L ++F  FP+YKN   F+TGE YAG Y+P LA  +V ++ S   +NL+ +++GN       +DN          L   R+  S  TH  +  + N   NK  +  T       +  +   ++    +++YN+YAP C    P
Sbjct:   99 GPFLVQPDGVTLEYNPYSWNLIANVLYLESPAGVGFSYSD--DKFYATNDTEVAQSNFEALQDFFRLFPEYKNNKLFLTGESYAGIYIPTLA-VLVMQDPS---MNLQGLAVGNGLSSYEQNDNSLVYFAYYHGLLGNRLWSSLQTHCCSQNKCNFYDNKDLECVT-------NLQEVARIVGNSGLNIYNLYAP-CAGGVP 286          

HSP 2 Score: 44.669 bits (104), Expect = 4.447e-28
Identity = 23/44 (52.27%), Postives = 26/44 (59.09%), Query Frame = 3
Query:   12 AKTLFYYFAESPHSSSTKPMVLWLNGGP*CSSLGYGCHGTTGPF 143
            +K L Y+F ES       P+VLWLNGGP CSSL  G     GPF
Sbjct:   59 SKHLHYWFVESQKDPENSPVVLWLNGGPGCSSLD-GLLTEHGPF 101          
BLAST of EY659305 vs. ExPASy Swiss-Prot
Match: YUA6_CAEEL (Uncharacterized serine carboxypeptidase F13S12.6 OS=Caenorhabditis elegans GN=F13D12.6 PE=2 SV=1)

HSP 1 Score: 103.99 bits (258), Expect = 9.820e-28
Identity = 53/141 (37.59%), Postives = 86/141 (60.99%), Query Frame = 1
Query:  118 AAMEQLGPFIVNSDG*TLYRNEYAWNNVANVLFLETPSRVRFSYSNTSSDYRNPGDNNTSDDSYTFLTNWFERFPQYKNRDFFITGEIYAGHYVPQLAHTIVSKNTSKTIINLKCISIGNAWIDDNLCTKRMLDSFWTHAL 540
            A + + GP+ VN+DG TL  N Y+WN  A++L LE P+ V +SY+  ++     GD+ T+ +++  L  +F  FPQYK  DF++TGE Y G YVP L  TI+ +  S++ IN+K ++IGN  +  N     +++  + H +
Sbjct:   83 ALLTEWGPWNVNTDGATLRTNPYSWNKNASILTLEAPAGVGYSYATDNNIAT--GDDQTASENWEALVAFFNEFPQYKGNDFYVTGESYGGIYVPTLVQTILDRQ-SQSHINIKGLAIGNGCVSANEGVDSLVNFLYHHGV 220          

HSP 2 Score: 40.4318 bits (93), Expect = 9.820e-28
Identity = 17/30 (56.67%), Postives = 21/30 (70.00%), Query Frame = 3
Query:   21 LFYYFAESPHSSSTKPMVLWLNGGP*CSSL 110
            L Y+F ES  + ST P++LWL GGP CS L
Sbjct:   52 LHYWFVESQSNPSTDPVLLWLTGGPGCSGL 81          
The following BLAST results are available for this feature:
BLAST of EY659305 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 91
Match NameE-valueIdentityDescription
SCP45_ARATH1.271e-3138.00Serine carboxypeptidase-like 45 OS=Arabidopsis tha... [more]
NF314_NAEFO2.746e-3135.52Virulence-related protein Nf314 OS=Naegleria fowle... [more]
SCP20_ARATH1.743e-3035.26Serine carboxypeptidase-like 20 OS=Arabidopsis tha... [more]
SCP53_ARATH2.398e-3062.37Putative serine carboxypeptidase-like 53 OS=Arabid... [more]
CBP21_HORVU3.462e-2936.32Serine carboxypeptidase II-1 (Fragment) OS=Hordeum... [more]
PPGB_BOVIN1.548e-2833.66Lysosomal protective protein OS=Bos taurus GN=CTSA... [more]
SCPL1_DICDI2.630e-2841.60Serine carboxypeptidase S10 family member 1 OS=Dic... [more]
PPGB_MOUSE3.417e-2833.66Lysosomal protective protein OS=Mus musculus GN=Ct... [more]
PPGB_HUMAN4.447e-2833.17Lysosomal protective protein OS=Homo sapiens GN=CT... [more]
YUA6_CAEEL9.820e-2837.59Uncharacterized serine carboxypeptidase F13S12.6 O... [more]

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Properties
Property NameValue
Genbank descriptionCS00-C1-101-008-F09-CT.F Sweet orange leaf, infected with Xylella fastidiosa (stage 1 of 2) Citrus sinensis cDNA, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>EY659305 ID=EY659305; Name=EY659305; organism=Citrus sinensis; type=EST; length=883bp
ACCCCAAGACTGCGAAGACATTGTTCTACTATTTCGCTGAGTCCCCTCAC
AGTTCCTCCACCAAACCTATGGTGTTATGGCTAAATGGAGGTCCATGATG
CTCATCTCTTGGATATGGCTGCCATGGAACAACTGGGCCCTTTCATAGTA
AACAGCGATGGCTAAACCCTATACAGAAATGAATATGCTTGGAATAACGT
GGCAAATGTGCTATTCTTGGAGACACCATCAAGAGTCCGGTTCTCATATT
CAAATACCTCTTCAGACTACAGGAATCCCGGCGACAATAACACATCTGAC
GATTCTTATACTTTCCTCACGAATTGGTTCGAGAGATTTCCCCAATACAA
AAACCGTGACTTCTTTATTACTGGTGAGATCTATGCTGGCCACTATGTCC
CTCAACTTGCTCACACCATTGTCTCCAAGAACACAAGTAAAACAATCATC
AACCTCAAATGCATATCTATTGGGAATGCTTGGATAGATGATAATTTGTG
CACCAAAAGAATGTTAGACTCTTTCTGGACACATGCTCTCAATTCCTATG
AAACCAATGCCGAGATTAACAAATATTGTGACTATGCGACCGGTCAACTG
TCAACTTCGTGTGATCAATATCAAACTCTATGAGTTATGGCAGTTAGACA
GATTGATCTATACAACGTTTACGCTCCACTTTGCAAATCATCAGCACCAC
CACCTCCAACAGCCCTCGTTATCAGAGAATATGACCCCTGCTCCGAAACG
TCGTTTATACCTATTAAACGCTTGCCCAGTGCGAGCAGCTCTTNATGCGA
TACATACAACTGGTCAACTCGCANCGACTAGACATGGACCGACAGCCATN
AACTGTGCTTCCACATACAGCAGCTGGTAGCAG
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