EY659290

Overview
NameEY659290
Unique NameEY659290
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length906
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of EY659290 vs. ExPASy Swiss-Prot
Match: DLDH_MANSE (Dihydrolipoyl dehydrogenase OS=Manduca sexta PE=2 SV=1)

HSP 1 Score: 79.7221 bits (195), Expect = 2.504e-14
Identity = 52/138 (37.68%), Postives = 77/138 (55.80%), Query Frame = 2 
Query:    2 DARSYGWEVHEINDFDWKKLLQKKTDEILRLNGIYKRLLSNAGVKLYEGEGKIVGPNEVEVTQLDGTKLSYSAKHILIATGSRAQRAPIPG----QELAITSDEALSLEELPKRAVVLGGGYIAVEFASIWRGMGSTV 403
            D +  G E  E+  F++K ++  K + +  L G    L     VKL  G G IV PN+VEV    G + + + K+ILIATGS     P PG    ++  ITS  ALSLE +PK+ +V+G G I +E  S+++ +G+ V
Sbjct:   93 DFKHRGIETGEVK-FNFKAMMDYKVNAVKALTGGIAMLFQKNKVKLVRGAGTIVAPNKVEVKGEKGVE-TVNTKNILIATGSEV--TPFPGVTFDEKQIITSTGALSLESVPKKMLVIGAGVIGLELGSVYQRLGADV 226
[Close]
BLAST of EY659290 vs. ExPASy Swiss-Prot
Match: DLDH_CANFA (Dihydrolipoyl dehydrogenase, mitochondrial OS=Canis familiaris GN=DLD PE=1 SV=1)

HSP 1 Score: 79.7221 bits (195), Expect = 2.504e-14
Identity = 48/138 (34.78%), Postives = 76/138 (55.07%), Query Frame = 2 
Query:    2 DARSYGWEVHEINDFDWKKLLQKKTDEILRLNGIYKRLLSNAGVKLYEGEGKIVGPNEVEVTQLDGTKLSYSAKHILIATGSRAQRAPIPG----QELAITSDEALSLEELPKRAVVLGGGYIAVEFASIWRGMGSTV 403
            D  S G E+ E+   + +K++++K+  +  L G    L     V    G GKI G N+V   + DG+      K+ILIATGS     P PG    ++  ++S  ALSL+++P++ VV+G G I VE  S+W+ +G+ V
Sbjct:  105 DFASRGIEMSEVR-LNLEKMMEQKSTAVKALTGGIAHLFKQNKVVHVNGYGKITGKNQVTAKKADGSTQVIDTKNILIATGSEV--TPFPGITIDEDTIVSSTGALSLKKVPEKMVVIGAGVIGVELGSVWQRLGADV 239
[Close]
BLAST of EY659290 vs. ExPASy Swiss-Prot
Match: TRXR1_CAEEL (Thioredoxin reductase 1 OS=Caenorhabditis elegans GN=trxr-1 PE=2 SV=3)

HSP 1 Score: 78.9518 bits (193), Expect = 4.270e-14
Identity = 51/153 (33.33%), Postives = 72/153 (47.06%), Query Frame = 2 
Query:    2 DARSYGWEVHEIN-DFDWKKLLQKKTDEILRLNGIYKRLLSNAGVKLYEGEGKIVGPNEVEVTQLDGTKLSYSAKHILIATGSRAQRAPIPG-QELAITSDEALSLEELPKRAVVLGGGYIAVEFASIWRGMGSTVDLLFRKELPLRGFDDEM 454
            DA+ YGW++ E   +  W  L     D I  LN  Y+  L    V      G+  GP E+  T         +A   LI+TG R +   IPG +E  ITSD+   L   P + + +G  Y+++E A    G G  V ++ R  L LRGFD +M
Sbjct:  242 DAKKYGWKLPEGKVEHQWNHLRDSVQDHIASLNWGYRVQLREKTVTYINSYGEFTGPFEISATNKKKKVEKLTADRFLISTGLRPKYPEIPGVKEYTITSDDLFQLPYSPGKTLCVGASYVSLECAGFLHGFGFDVTVMVRSIL-LRGFDQDM 393
[Close]
BLAST of EY659290 vs. ExPASy Swiss-Prot
Match: STHA_MYCTU (Probable soluble pyridine nucleotide transhydrogenase OS=Mycobacterium tuberculosis GN=sthA PE=3 SV=1)

HSP 1 Score: 54.6842 bits (130), Expect = 6.414e-14
Identity = 36/121 (29.75%), Postives = 63/121 (52.07%), Query Frame = 2 
Query:  101 IYKRLLSNAGVKLYEGEGKIVGPNEVEVT-QLDGTKLSYSAKHILIATGSRAQRAPIP--GQELAITSDEALSLEELPKRAVVLGGGYIAVEFASIWRGMGSTVDLLFRKELPLRGFDDEM 454
            + +  L    V L  G G+ + P+ + V  Q    K + +  +I+IATG+R  R       +E  + SD  L L+ LP   VV+G G I +E+AS++  +G+ V ++ +++  L   D E+
Sbjct:   98 VVRNQLMRNRVDLIVGHGRFIDPHTILVEDQARREKTTVTGDYIIIATGTRPARPSGVEFDEERVLDSDGILDLKSLPSSMVVVGAGVIGIEYASMFAALGTKVTVVEKRDNMLDFCDPEV 218

HSP 2 Score: 43.8986 bits (102), Expect = 6.414e-14
Identity = 22/55 (40.00%), Postives = 33/55 (60.00%), Query Frame = 1 
Query:  565 GEEIVADVVLFATGRAPNTKRLNLKAVVVEVDQTGAVEVDDNSRTNVPSIWAVGD 729
            G++I A+ V+++ GR   T  L+L    +EV   G + VDD  +T V  I+AVGD
Sbjct:  256 GKQIPAETVMYSAGRQGQTDHLDLHNAGLEVQGRGRIFVDDRFQTKVDHIYAVGD 310
[Close]
BLAST of EY659290 vs. ExPASy Swiss-Prot
Match: STHA_MYCTA (Soluble pyridine nucleotide transhydrogenase OS=Mycobacterium tuberculosis (strain ATCC 25177 / H37Ra) GN=sthA PE=3 SV=1)

HSP 1 Score: 54.6842 bits (130), Expect = 6.414e-14
Identity = 36/121 (29.75%), Postives = 63/121 (52.07%), Query Frame = 2 
Query:  101 IYKRLLSNAGVKLYEGEGKIVGPNEVEVT-QLDGTKLSYSAKHILIATGSRAQRAPIP--GQELAITSDEALSLEELPKRAVVLGGGYIAVEFASIWRGMGSTVDLLFRKELPLRGFDDEM 454
            + +  L    V L  G G+ + P+ + V  Q    K + +  +I+IATG+R  R       +E  + SD  L L+ LP   VV+G G I +E+AS++  +G+ V ++ +++  L   D E+
Sbjct:   98 VVRNQLMRNRVDLIVGHGRFIDPHTILVEDQARREKTTVTGDYIIIATGTRPARPSGVEFDEERVLDSDGILDLKSLPSSMVVVGAGVIGIEYASMFAALGTKVTVVEKRDNMLDFCDPEV 218

HSP 2 Score: 43.8986 bits (102), Expect = 6.414e-14
Identity = 22/55 (40.00%), Postives = 33/55 (60.00%), Query Frame = 1 
Query:  565 GEEIVADVVLFATGRAPNTKRLNLKAVVVEVDQTGAVEVDDNSRTNVPSIWAVGD 729
            G++I A+ V+++ GR   T  L+L    +EV   G + VDD  +T V  I+AVGD
Sbjct:  256 GKQIPAETVMYSAGRQGQTDHLDLHNAGLEVQGRGRIFVDDRFQTKVDHIYAVGD 310
[Close]
BLAST of EY659290 vs. ExPASy Swiss-Prot
Match: STHA_MYCBT (Soluble pyridine nucleotide transhydrogenase OS=Mycobacterium bovis (strain BCG / Tokyo 172 / ATCC 35737 / TMC 1019) GN=sthA PE=3 SV=1)

HSP 1 Score: 54.6842 bits (130), Expect = 6.414e-14
Identity = 36/121 (29.75%), Postives = 63/121 (52.07%), Query Frame = 2 
Query:  101 IYKRLLSNAGVKLYEGEGKIVGPNEVEVT-QLDGTKLSYSAKHILIATGSRAQRAPIP--GQELAITSDEALSLEELPKRAVVLGGGYIAVEFASIWRGMGSTVDLLFRKELPLRGFDDEM 454
            + +  L    V L  G G+ + P+ + V  Q    K + +  +I+IATG+R  R       +E  + SD  L L+ LP   VV+G G I +E+AS++  +G+ V ++ +++  L   D E+
Sbjct:   98 VVRNQLMRNRVDLIVGHGRFIDPHTILVEDQARREKTTVTGDYIIIATGTRPARPSGVEFDEERVLDSDGILDLKSLPSSMVVVGAGVIGIEYASMFAALGTKVTVVEKRDNMLDFCDPEV 218

HSP 2 Score: 43.8986 bits (102), Expect = 6.414e-14
Identity = 22/55 (40.00%), Postives = 33/55 (60.00%), Query Frame = 1 
Query:  565 GEEIVADVVLFATGRAPNTKRLNLKAVVVEVDQTGAVEVDDNSRTNVPSIWAVGD 729
            G++I A+ V+++ GR   T  L+L    +EV   G + VDD  +T V  I+AVGD
Sbjct:  256 GKQIPAETVMYSAGRQGQTDHLDLHNAGLEVQGRGRIFVDDRFQTKVDHIYAVGD 310
[Close]
BLAST of EY659290 vs. ExPASy Swiss-Prot
Match: STHA_MYCBP (Soluble pyridine nucleotide transhydrogenase OS=Mycobacterium bovis (strain BCG / Pasteur 1173P2) GN=sthA PE=3 SV=1)

HSP 1 Score: 54.6842 bits (130), Expect = 6.414e-14
Identity = 36/121 (29.75%), Postives = 63/121 (52.07%), Query Frame = 2 
Query:  101 IYKRLLSNAGVKLYEGEGKIVGPNEVEVT-QLDGTKLSYSAKHILIATGSRAQRAPIP--GQELAITSDEALSLEELPKRAVVLGGGYIAVEFASIWRGMGSTVDLLFRKELPLRGFDDEM 454
            + +  L    V L  G G+ + P+ + V  Q    K + +  +I+IATG+R  R       +E  + SD  L L+ LP   VV+G G I +E+AS++  +G+ V ++ +++  L   D E+
Sbjct:   98 VVRNQLMRNRVDLIVGHGRFIDPHTILVEDQARREKTTVTGDYIIIATGTRPARPSGVEFDEERVLDSDGILDLKSLPSSMVVVGAGVIGIEYASMFAALGTKVTVVEKRDNMLDFCDPEV 218

HSP 2 Score: 43.8986 bits (102), Expect = 6.414e-14
Identity = 22/55 (40.00%), Postives = 33/55 (60.00%), Query Frame = 1 
Query:  565 GEEIVADVVLFATGRAPNTKRLNLKAVVVEVDQTGAVEVDDNSRTNVPSIWAVGD 729
            G++I A+ V+++ GR   T  L+L    +EV   G + VDD  +T V  I+AVGD
Sbjct:  256 GKQIPAETVMYSAGRQGQTDHLDLHNAGLEVQGRGRIFVDDRFQTKVDHIYAVGD 310
[Close]
BLAST of EY659290 vs. ExPASy Swiss-Prot
Match: STHA_MYCBO (Probable soluble pyridine nucleotide transhydrogenase OS=Mycobacterium bovis GN=sthA PE=3 SV=1)

HSP 1 Score: 54.6842 bits (130), Expect = 6.414e-14
Identity = 36/121 (29.75%), Postives = 63/121 (52.07%), Query Frame = 2 
Query:  101 IYKRLLSNAGVKLYEGEGKIVGPNEVEVT-QLDGTKLSYSAKHILIATGSRAQRAPIP--GQELAITSDEALSLEELPKRAVVLGGGYIAVEFASIWRGMGSTVDLLFRKELPLRGFDDEM 454
            + +  L    V L  G G+ + P+ + V  Q    K + +  +I+IATG+R  R       +E  + SD  L L+ LP   VV+G G I +E+AS++  +G+ V ++ +++  L   D E+
Sbjct:   98 VVRNQLMRNRVDLIVGHGRFIDPHTILVEDQARREKTTVTGDYIIIATGTRPARPSGVEFDEERVLDSDGILDLKSLPSSMVVVGAGVIGIEYASMFAALGTKVTVVEKRDNMLDFCDPEV 218

HSP 2 Score: 43.8986 bits (102), Expect = 6.414e-14
Identity = 22/55 (40.00%), Postives = 33/55 (60.00%), Query Frame = 1 
Query:  565 GEEIVADVVLFATGRAPNTKRLNLKAVVVEVDQTGAVEVDDNSRTNVPSIWAVGD 729
            G++I A+ V+++ GR   T  L+L    +EV   G + VDD  +T V  I+AVGD
Sbjct:  256 GKQIPAETVMYSAGRQGQTDHLDLHNAGLEVQGRGRIFVDDRFQTKVDHIYAVGD 310
[Close]
BLAST of EY659290 vs. ExPASy Swiss-Prot
Match: DLDH_TRYBB (Dihydrolipoyl dehydrogenase OS=Trypanosoma brucei brucei PE=3 SV=1)

HSP 1 Score: 77.0258 bits (188), Expect = 1.623e-13
Identity = 45/124 (36.29%), Postives = 70/124 (56.45%), Query Frame = 2 
Query:   47 DWKKLLQKKTDEILRLNGIYKRLLSNAGVKLYEGEGKIVGPNEVEVTQLDGTKLSYSAKHILIATGSRAQRAP-IPGQE-LAITSDEALSLEELPKRAVVLGGGYIAVEFASIWRGMGSTVDLL 412
            D  K+ Q+K   +  L    + LL    V  Y+GE   V PN + V  +DG   +  AK+ +IATGS     P +P  E + ++S  AL+L+++PK+ VV+GGG I +E  S+W  +GS V ++
Sbjct:   89 DVAKMQQQKEKSVNGLTSGVEYLLKKNKVTYYKGEAGFVTPNTLNVKGIDGKDEAIEAKNTIIATGSEPTALPFLPFDEKVVLSSTGALALQQVPKKMVVIGGGVIGLELGSVWARLGSDVTVV 212
[Close]
BLAST of EY659290 vs. ExPASy Swiss-Prot
Match: IRC15_YEAST (Increased recombination centers protein 15 OS=Saccharomyces cerevisiae GN=IRC15 PE=1 SV=1)

HSP 1 Score: 76.2554 bits (186), Expect = 2.768e-13
Identity = 45/139 (32.37%), Postives = 71/139 (51.08%), Query Frame = 2 
Query:   44 FDWKKLLQKKTDEILRLNGIYKRLLSNAGVKLYEGEGKIVGPNEVEVTQLDGTKLSYSAKHILIATGSRAQRAP--IPGQELAITSDEALSLEELPKRAVVLGGGYIAVEFASIWRGMGSTVDLLFRKELPLRGFDDEM 454
            FD +         I  L  +YKR LS   V +Y+G      P+ VE+ Q         AK+I++ATGS   + P      +  I+SD+ALSL+ +P R  ++GGG I +E A I+  +GS V ++  +    +  D+E+
Sbjct:   95 FDMQAAQSALKHNIEELGNVYKRELSKNNVTVYKGTAAFKDPHHVEIAQRGMKPFIVEAKYIVVATGSAVIQCPGVAIDNDKIISSDKALSLDYIPSRFTIMGGGTIGLEIACIFNNLGSRVTIVESQSEICQNMDNEL 233
The following BLAST results are available for this feature:
BLAST of EY659290 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 178
Match NameE-valueIdentityDescription
DLDH_MANSE2.504e-1437.68Dihydrolipoyl dehydrogenase OS=Manduca sexta PE=2 ... [more]
DLDH_CANFA2.504e-1434.78Dihydrolipoyl dehydrogenase, mitochondrial OS=Cani... [more]
TRXR1_CAEEL4.270e-1433.33Thioredoxin reductase 1 OS=Caenorhabditis elegans ... [more]
STHA_MYCTU6.414e-1429.75Probable soluble pyridine nucleotide transhydrogen... [more]
STHA_MYCTA6.414e-1429.75Soluble pyridine nucleotide transhydrogenase OS=My... [more]
STHA_MYCBT6.414e-1429.75Soluble pyridine nucleotide transhydrogenase OS=My... [more]
STHA_MYCBP6.414e-1429.75Soluble pyridine nucleotide transhydrogenase OS=My... [more]
STHA_MYCBO6.414e-1429.75Probable soluble pyridine nucleotide transhydrogen... [more]
DLDH_TRYBB1.623e-1336.29Dihydrolipoyl dehydrogenase OS=Trypanosoma brucei ... [more]
IRC15_YEAST2.768e-1332.37Increased recombination centers protein 15 OS=Sacc... [more]

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Properties
Property NameValue
Genbank descriptionCS00-C1-101-008-E04-CT.F Sweet orange leaf, infected with Xylella fastidiosa (stage 1 of 2) Citrus sinensis cDNA, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>EY659290 ID=EY659290; Name=EY659290; organism=Citrus sinensis; type=EST; length=906bp
AGATGCAAGGAGTTATGGATGGGAGGTACATGAGATAAATGACTTCGACT
GGAAAAAACTTCTGCAAAAGAAGACAGATGAAATACTCAGACTAAATGGA
ATTTACAAGCGATTATTATCTAATGCTGGGGTAAAATTATATGAAGGGGA
GGGAAAGATAGTTGGTCCAAATGAAGTTGAGGTCACACAACTAGATGGCA
CTAAGTTAAGTTACTCAGCAAAGCACATATTGATCGCAACTGGAAGTAGG
GCTCAACGTGCTCCTATTCCTGGCCAGGAGCTGGCTATAACCTCTGACGA
GGCATTGAGCTTGGAAGAATTACCCAAGCGTGCTGTGGTGCTTGGTGGAG
GGTACATTGCTGTTGAGTTTGCTTCCATATGGCGAGGGATGGGTTCTACT
GTGGATCTATTATTTAGAAAGGAACTTCCATTAAGAGGGTTTGATGATGA
AATGCGGGCAGTAGTTGCCGAAATCTGGAAGGGAGGGGAATTAATTTGCA
CCCGAGGACTACCATAAAGGAGTTGATTAAGTCAGAGGAGAGTATAAAAG
TTATTACAGATCATGGTGAAGAGATTGTTGCAGATGTTGTACTCTTTGCC
ACTGGCAGGGCACCTAATACAAAAAGGTTGAATTTGAAAGCTGTTGTGGT
GGAAGTTGATCAAACAGGAGCTGTGGAGGTGGACGATAACTCACGCACCA
ACGTACCTAGTATATGGGCTGTGGGTGATGGAACTAATCGAATGAATCCT
ACCCCTGCGGCTTGATGGAGGGAACATGCTTTGCAAAAACTGTTTTTGGG
GGACACCCTGCAAACCAACTACGGGATGGACCCTGGGCTGGATTTGGCTA
CCTCCTTCTTTGGGAGGGGCCCCATGGAGGGACAGGCCTTAACCAGGAAA
TGGTGT
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