EY659233 | Citrus Genome Database

Overview


Name	EY659233
Unique Name	EY659233
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	817

Libraries


Library Name	Type
Sweet orange leaf, infected with Xylella fastidiosa (stage 1 of 2)	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Homology

[Close]

BLAST of EY659233 vs. ExPASy Swiss-Prot
Match: UBC2_CAEEL (Ubiquitin-conjugating enzyme E2 2 OS=Caenorhabditis elegans GN=let-70 PE=1 SV=1)

HSP 1 Score: 100.523 bits (249), Expect = 6.536e-37
Identity = 43/56 (76.79%), Postives = 51/56 (91.07%), Query Frame = 2

Query:  338 GVFLVAINFPPDYPFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKMV 505
            GVF + I+FP DYPFKPPKVAF T+++HPNINSNGSICLDIL+ QWSPALTISK++
Sbjct:   48 GVFFLTIHFPTDYPFKPPKVAFTTRIYHPNINSNGSICLDILRSQWSPALTISKVL 103

HSP 2 Score: 75.485 bits (184), Expect = 6.536e-37
Identity = 32/45 (71.11%), Postives = 37/45 (82.22%), Query Frame = 3

Query:  192 MASKRILKELKDLQKDPPTSCSAGPAAEDMFHWQATIMGPSDSPY 326
            MA KRI KEL+DL +DPP  CSAGP  +D+FHWQATIMGP +SPY
Sbjct:    1 MALKRIQKELQDLGRDPPAQCSAGPVGDDLFHWQATIMGPPESPY 45

[Close]

BLAST of EY659233 vs. ExPASy Swiss-Prot
Match: U2D2B_RAT (Ubiquitin-conjugating enzyme E2 D2B OS=Rattus norvegicus GN=Ube2d2b PE=2 SV=1)

HSP 1 Score: 95.1301 bits (235), Expect = 1.882e-36
Identity = 39/56 (69.64%), Postives = 49/56 (87.50%), Query Frame = 2

Query:  338 GVFLVAINFPPDYPFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKMV 505
            G F + I+FP +YPFKPPKV F T+++HPN+NSNGSICLDIL+ QWSPALTISK++
Sbjct:   48 GAFFLTIDFPTEYPFKPPKVEFTTRIYHPNVNSNGSICLDILRSQWSPALTISKVL 103

HSP 2 Score: 79.337 bits (194), Expect = 1.882e-36
Identity = 35/45 (77.78%), Postives = 37/45 (82.22%), Query Frame = 3

Query:  192 MASKRILKELKDLQKDPPTSCSAGPAAEDMFHWQATIMGPSDSPY 326
            MA KRI KEL DL +DPP  CSAGP  EDMFHWQATIMGP+DSPY
Sbjct:    1 MALKRIHKELNDLAQDPPAQCSAGPVGEDMFHWQATIMGPNDSPY 45

[Close]

BLAST of EY659233 vs. ExPASy Swiss-Prot
Match: U2D2B_MOUSE (Ubiquitin-conjugating enzyme E2 D2B OS=Mus musculus GN=Ube2d2b PE=2 SV=1)

HSP 1 Score: 95.1301 bits (235), Expect = 1.882e-36
Identity = 39/56 (69.64%), Postives = 49/56 (87.50%), Query Frame = 2

Query:  338 GVFLVAINFPPDYPFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKMV 505
            G F + I+FP +YPFKPPKV F T+++HPN+NSNGSICLDIL+ QWSPALTISK++
Sbjct:   48 GAFFLTIDFPTEYPFKPPKVEFTTRIYHPNVNSNGSICLDILRSQWSPALTISKVL 103

HSP 2 Score: 79.337 bits (194), Expect = 1.882e-36
Identity = 35/45 (77.78%), Postives = 37/45 (82.22%), Query Frame = 3

Query:  192 MASKRILKELKDLQKDPPTSCSAGPAAEDMFHWQATIMGPSDSPY 326
            MA KRI KEL DL +DPP  CSAGP  EDMFHWQATIMGP+DSPY
Sbjct:    1 MALKRIHKELNDLAQDPPAQCSAGPVGEDMFHWQATIMGPNDSPY 45

[Close]

BLAST of EY659233 vs. ExPASy Swiss-Prot
Match: UBC4_CANAL (Ubiquitin-conjugating enzyme E2 4 OS=Candida albicans GN=UBC4 PE=2 SV=1)

HSP 1 Score: 97.8265 bits (242), Expect = 3.195e-36
Identity = 41/56 (73.21%), Postives = 51/56 (91.07%), Query Frame = 2

Query:  338 GVFLVAINFPPDYPFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKMV 505
            GVF ++I+FP DYP KPPK+A  TK++HPNINSNG+ICLDILK+QWSPALTISK++
Sbjct:   48 GVFFLSIHFPTDYPLKPPKIALTTKIYHPNINSNGNICLDILKDQWSPALTISKVL 103

HSP 2 Score: 75.8702 bits (185), Expect = 3.195e-36
Identity = 32/46 (69.57%), Postives = 39/46 (84.78%), Query Frame = 3

Query:  192 MASKRILKELKDLQKDPPTSCSAGPAAEDMFHWQATIMGPSDSPYA 329
            M+ KRI KEL DL +DPP+SCSAGP  +D++HWQA+IMGP DSPYA
Sbjct:    1 MSLKRINKELSDLGRDPPSSCSAGPVGDDLYHWQASIMGPPDSPYA 46

[Close]

BLAST of EY659233 vs. ExPASy Swiss-Prot
Match: UBC12_ARATH (Probable ubiquitin-conjugating enzyme E2 12 OS=Arabidopsis thaliana GN=UBC12 PE=2 SV=1)

HSP 1 Score: 93.2041 bits (230), Expect = 5.850e-35
Identity = 42/56 (75.00%), Postives = 49/56 (87.50%), Query Frame = 2

Query:  338 GVFLVAINFPPDYPFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKMV 505
            GVF V+I+F  DYPFKPPKV F+TKV+HPNI+S GSICLDILKEQWSPA T SK++
Sbjct:   49 GVFTVSIDFSSDYPFKPPKVNFKTKVYHPNIDSKGSICLDILKEQWSPAPTTSKVL 104

HSP 2 Score: 76.2554 bits (186), Expect = 5.850e-35
Identity = 34/47 (72.34%), Postives = 41/47 (87.23%), Query Frame = 3

Query:  192 MASKRILKELKDLQKDPPTSCSAGPAA-EDMFHWQATIMGPSDSPYA 329
            MASKRI +EL+D+Q+ PP +CSAGP A ED+FHWQATIMGP DSPY+
Sbjct:    1 MASKRISRELRDMQRHPPANCSAGPVAEEDIFHWQATIMGPHDSPYS 47

[Close]

BLAST of EY659233 vs. ExPASy Swiss-Prot
Match: UB2E2_MOUSE (Ubiquitin-conjugating enzyme E2 E2 OS=Mus musculus GN=Ube2e2 PE=2 SV=1)

HSP 1 Score: 91.6633 bits (226), Expect = 7.485e-28
Identity = 40/56 (71.43%), Postives = 47/56 (83.93%), Query Frame = 2

Query:  338 GVFLVAINFPPDYPFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKMV 505
            GVF + I F PDYPFKPPKV FRT+++H NINS G ICLDILK+ WSPALTISK++
Sbjct:  102 GVFFLDITFSPDYPFKPPKVTFRTRIYHCNINSQGVICLDILKDNWSPALTISKVL 157

HSP 2 Score: 53.9138 bits (128), Expect = 7.485e-28
Identity = 21/44 (47.73%), Postives = 33/44 (75.00%), Query Frame = 3

Query:  195 ASKRILKELKDLQKDPPTSCSAGPAAEDMFHWQATIMGPSDSPY 326
            ++KRI KEL ++  DPP +CSAGP  ++++ W++TI+GP  S Y
Sbjct:   56 SAKRIQKELAEITLDPPPNCSAGPKGDNIYEWRSTILGPPGSVY 99

[Close]

BLAST of EY659233 vs. ExPASy Swiss-Prot
Match: UB2E2_HUMAN (Ubiquitin-conjugating enzyme E2 E2 OS=Homo sapiens GN=UBE2E2 PE=1 SV=1)

HSP 1 Score: 91.6633 bits (226), Expect = 7.485e-28
Identity = 40/56 (71.43%), Postives = 47/56 (83.93%), Query Frame = 2

Query:  338 GVFLVAINFPPDYPFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKMV 505
            GVF + I F PDYPFKPPKV FRT+++H NINS G ICLDILK+ WSPALTISK++
Sbjct:  102 GVFFLDITFSPDYPFKPPKVTFRTRIYHCNINSQGVICLDILKDNWSPALTISKVL 157

HSP 2 Score: 53.9138 bits (128), Expect = 7.485e-28
Identity = 21/44 (47.73%), Postives = 33/44 (75.00%), Query Frame = 3

Query:  195 ASKRILKELKDLQKDPPTSCSAGPAAEDMFHWQATIMGPSDSPY 326
            ++KRI KEL ++  DPP +CSAGP  ++++ W++TI+GP  S Y
Sbjct:   56 SAKRIQKELAEITLDPPPNCSAGPKGDNIYEWRSTILGPPGSVY 99

[Close]

BLAST of EY659233 vs. ExPASy Swiss-Prot
Match: UB2E1_MOUSE (Ubiquitin-conjugating enzyme E2 E1 OS=Mus musculus GN=Ube2e1 PE=2 SV=1)

HSP 1 Score: 90.1225 bits (222), Expect = 7.496e-28
Identity = 39/56 (69.64%), Postives = 47/56 (83.93%), Query Frame = 2

Query:  338 GVFLVAINFPPDYPFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKMV 505
            GVF + I F P+YPFKPPKV FRT+++H NINS G ICLDILK+ WSPALTISK++
Sbjct:   94 GVFFLDITFTPEYPFKPPKVTFRTRIYHCNINSQGVICLDILKDNWSPALTISKVL 149

HSP 2 Score: 55.4546 bits (132), Expect = 7.496e-28
Identity = 22/44 (50.00%), Postives = 33/44 (75.00%), Query Frame = 3

Query:  195 ASKRILKELKDLQKDPPTSCSAGPAAEDMFHWQATIMGPSDSPY 326
            ++KRI KEL D+  DPP +CSAGP  ++++ W++TI+GP  S Y
Sbjct:   48 SAKRIQKELADITLDPPPNCSAGPKGDNIYEWRSTILGPPGSVY 91

[Close]

BLAST of EY659233 vs. ExPASy Swiss-Prot
Match: UB2E1_HUMAN (Ubiquitin-conjugating enzyme E2 E1 OS=Homo sapiens GN=UBE2E1 PE=1 SV=1)

HSP 1 Score: 90.1225 bits (222), Expect = 7.496e-28
Identity = 39/56 (69.64%), Postives = 47/56 (83.93%), Query Frame = 2

Query:  338 GVFLVAINFPPDYPFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKMV 505
            GVF + I F P+YPFKPPKV FRT+++H NINS G ICLDILK+ WSPALTISK++
Sbjct:   94 GVFFLDITFTPEYPFKPPKVTFRTRIYHCNINSQGVICLDILKDNWSPALTISKVL 149

HSP 2 Score: 55.4546 bits (132), Expect = 7.496e-28
Identity = 22/44 (50.00%), Postives = 33/44 (75.00%), Query Frame = 3

Query:  195 ASKRILKELKDLQKDPPTSCSAGPAAEDMFHWQATIMGPSDSPY 326
            ++KRI KEL D+  DPP +CSAGP  ++++ W++TI+GP  S Y
Sbjct:   48 SAKRIQKELADITLDPPPNCSAGPKGDNIYEWRSTILGPPGSVY 91

[Close]

BLAST of EY659233 vs. ExPASy Swiss-Prot
Match: UBCD2_DROME (Ubiquitin-conjugating enzyme E2-24 kDa OS=Drosophila melanogaster GN=UbcD2 PE=1 SV=1)

HSP 1 Score: 90.5077 bits (223), Expect = 2.789e-27
Identity = 39/56 (69.64%), Postives = 48/56 (85.71%), Query Frame = 2

Query:  338 GVFLVAINFPPDYPFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKMV 505
            GVF + I+F P+YPFKPPKV FRT+++H NINS G ICLDILK+ WSPALTISK++
Sbjct:  133 GVFFLDIHFSPEYPFKPPKVTFRTRIYHCNINSQGVICLDILKDNWSPALTISKVL 188

HSP 2 Score: 53.1434 bits (126), Expect = 2.789e-27
Identity = 21/44 (47.73%), Postives = 32/44 (72.73%), Query Frame = 3

Query:  195 ASKRILKELKDLQKDPPTSCSAGPAAEDMFHWQATIMGPSDSPY 326
            ++KRI KEL ++  DPP +CSAGP  ++++ W +TI+GP  S Y
Sbjct:   87 SAKRIQKELAEITLDPPPNCSAGPKGDNLYEWVSTILGPPGSVY 130

The following BLAST results are available for this feature:

BLAST of EY659233 vs. ExPASy Swiss-Prot

BLAST of EY659233 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 160


Match Name	E-value	Identity	Description
UBC2_CAEEL	6.536e-37	76.79	Ubiquitin-conjugating enzyme E2 2 OS=Caenorhabditi...	[more]
U2D2B_RAT	1.882e-36	69.64	Ubiquitin-conjugating enzyme E2 D2B OS=Rattus norv...	[more]
U2D2B_MOUSE	1.882e-36	69.64	Ubiquitin-conjugating enzyme E2 D2B OS=Mus musculu...	[more]
UBC4_CANAL	3.195e-36	73.21	Ubiquitin-conjugating enzyme E2 4 OS=Candida albic...	[more]
UBC12_ARATH	5.850e-35	75.00	Probable ubiquitin-conjugating enzyme E2 12 OS=Ara...	[more]
UB2E2_MOUSE	7.485e-28	71.43	Ubiquitin-conjugating enzyme E2 E2 OS=Mus musculus...	[more]
UB2E2_HUMAN	7.485e-28	71.43	Ubiquitin-conjugating enzyme E2 E2 OS=Homo sapiens...	[more]
UB2E1_MOUSE	7.496e-28	69.64	Ubiquitin-conjugating enzyme E2 E1 OS=Mus musculus...	[more]
UB2E1_HUMAN	7.496e-28	69.64	Ubiquitin-conjugating enzyme E2 E1 OS=Homo sapiens...	[more]
UBCD2_DROME	2.789e-27	69.64	Ubiquitin-conjugating enzyme E2-24 kDa OS=Drosophi...	[more]

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Properties


Property Name	Value
Genbank description	CS00-C1-101-004-H03-CT.F Sweet orange leaf, infected with Xylella fastidiosa (stage 1 of 2) Citrus sinensis cDNA, mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>EY659233 ID=EY659233|Name=EY659233|organism=Citrus sinensis|type=EST|length=817bp
TCTTTCTCGACGATATTTGTGTTGAAGAACAAAACAGGGGTTGTTAGAGG
AGCGAGGCGGGGGCGGCTTGACGACGTAGATCTGCTTCACCGTTTCGCTT
TCTCGACGCTATTACCCTACGATCCTGTTCTCTGCCGCGCAAGGTCTTAA
GGTTCGTTGTAGCTTTAATTTTAATAACCCCTTGAAGAGTCATGGCTTCA
AAGCGTATTTTGAAGGAACTCAAGGATCTGCAAAAGGATCCTCCCACATC
TTGCAGCGCTGGCCCTGCGGCTGAAGACATGTTCCACTGGCAAGCCACAA
TTATGGGCCCTTCTGATAGCCCATATGCTGATATGCAGGTGTATTTTTAG
TTGCCATTAATTTTCCTCCTGATTATCCTTTCAAGCCCCCAAAGGTTGCT
TTTAGGACCAAGGTCTTCCACCCGAACATCAACAGCAATGGAAGCATCTG
TCTTGATATCCTTAAAGAACAGTGGAGTCCTGCGCTTACCATTTCAAAGA
TGGTGAAGTTGTAAACGAATGCGCGATATAGTGCGGACGATGATTGGTTG
TTGAATACATTTCATGTGATATTGGCCATTCTCAGAACCTAACTCACTCT
GTAAATGCTTGAGCCTTTTGACTAAAGATTATTCAATGTATTGCCGCTGG
AAGATGGTATTGCTNCTGGNAAGTCTGCTGNTGGAATATTTNCATTTTAT
CTTGGNAAGTNNNTGTGGAGAGCTTGGCTCGGTGGCCGGAATTTNNGATA
CTNNTATGGNNAGTTAATGGGAATTTAACTATNGTTTTATATTTGGCATT
CCTTTTTTTTTTATTTA