EY675113 | Citrus Genome Database

Overview


Name	EY675113
Unique Name	EY675113
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	988

Libraries


Library Name	Type
Sweet orange leaf, infected with CiLV	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Homology

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BLAST of EY675113 vs. ExPASy Swiss-Prot
Match: UBE2B_RABIT (Ubiquitin-conjugating enzyme E2 B OS=Oryctolagus cuniculus GN=UBE2B PE=2 SV=1)

HSP 1 Score: 169.859 bits (429), Expect = 8.323e-62
Identity = 73/94 (77.66%), Postives = 88/94 (93.62%), Query Frame = 2

Query:   89 MSTPAKKRLMRDFKRLQQDPPAGISGAPQDNNIMLWNAVIFGPDDTPWDGGTFKLTLQFTEDYPNKPPTVRFVSRMFHPNIYADGSICLDILQN 370
            MSTPA++RLMRDFKRLQ+DPP G+SGAP +NNIM WNAVIFGP+ TP++ GTFKL ++F+E+YPNKPPTVRF+S+MFHPN+YADGSICLDILQN
Sbjct:    1 MSTPARRRLMRDFKRLQEDPPVGVSGAPSENNIMQWNAVIFGPEGTPFEDGTFKLVIEFSEEYPNKPPTVRFLSKMFHPNVYADGSICLDILQN 94

HSP 2 Score: 89.7373 bits (221), Expect = 8.323e-62
Identity = 41/54 (75.93%), Postives = 48/54 (88.89%), Query Frame = 3

Query:  375 WSPIYDVAAILTSIQSLLCDPNPNSPANSEAARLFSENKREYNRRVREIVEQSW 536
            WSP YDV++ILTSIQSLL +PNPNSPANS+AA+L+ ENKREY +RV  IVEQSW
Sbjct:   96 WSPTYDVSSILTSIQSLLDEPNPNSPANSQAAQLYQENKREYEKRVSAIVEQSW 149

[Close]

BLAST of EY675113 vs. ExPASy Swiss-Prot
Match: UBE2B_MOUSE (Ubiquitin-conjugating enzyme E2 B OS=Mus musculus GN=Ube2b PE=1 SV=1)

HSP 1 Score: 169.859 bits (429), Expect = 8.323e-62
Identity = 73/94 (77.66%), Postives = 88/94 (93.62%), Query Frame = 2

Query:   89 MSTPAKKRLMRDFKRLQQDPPAGISGAPQDNNIMLWNAVIFGPDDTPWDGGTFKLTLQFTEDYPNKPPTVRFVSRMFHPNIYADGSICLDILQN 370
            MSTPA++RLMRDFKRLQ+DPP G+SGAP +NNIM WNAVIFGP+ TP++ GTFKL ++F+E+YPNKPPTVRF+S+MFHPN+YADGSICLDILQN
Sbjct:    1 MSTPARRRLMRDFKRLQEDPPVGVSGAPSENNIMQWNAVIFGPEGTPFEDGTFKLVIEFSEEYPNKPPTVRFLSKMFHPNVYADGSICLDILQN 94

HSP 2 Score: 89.7373 bits (221), Expect = 8.323e-62
Identity = 41/54 (75.93%), Postives = 48/54 (88.89%), Query Frame = 3

Query:  375 WSPIYDVAAILTSIQSLLCDPNPNSPANSEAARLFSENKREYNRRVREIVEQSW 536
            WSP YDV++ILTSIQSLL +PNPNSPANS+AA+L+ ENKREY +RV  IVEQSW
Sbjct:   96 WSPTYDVSSILTSIQSLLDEPNPNSPANSQAAQLYQENKREYEKRVSAIVEQSW 149

[Close]

BLAST of EY675113 vs. ExPASy Swiss-Prot
Match: UBE2B_HUMAN (Ubiquitin-conjugating enzyme E2 B OS=Homo sapiens GN=UBE2B PE=1 SV=1)

HSP 1 Score: 169.859 bits (429), Expect = 8.323e-62
Identity = 73/94 (77.66%), Postives = 88/94 (93.62%), Query Frame = 2

Query:   89 MSTPAKKRLMRDFKRLQQDPPAGISGAPQDNNIMLWNAVIFGPDDTPWDGGTFKLTLQFTEDYPNKPPTVRFVSRMFHPNIYADGSICLDILQN 370
            MSTPA++RLMRDFKRLQ+DPP G+SGAP +NNIM WNAVIFGP+ TP++ GTFKL ++F+E+YPNKPPTVRF+S+MFHPN+YADGSICLDILQN
Sbjct:    1 MSTPARRRLMRDFKRLQEDPPVGVSGAPSENNIMQWNAVIFGPEGTPFEDGTFKLVIEFSEEYPNKPPTVRFLSKMFHPNVYADGSICLDILQN 94

HSP 2 Score: 89.7373 bits (221), Expect = 8.323e-62
Identity = 41/54 (75.93%), Postives = 48/54 (88.89%), Query Frame = 3

Query:  375 WSPIYDVAAILTSIQSLLCDPNPNSPANSEAARLFSENKREYNRRVREIVEQSW 536
            WSP YDV++ILTSIQSLL +PNPNSPANS+AA+L+ ENKREY +RV  IVEQSW
Sbjct:   96 WSPTYDVSSILTSIQSLLDEPNPNSPANSQAAQLYQENKREYEKRVSAIVEQSW 149

[Close]

BLAST of EY675113 vs. ExPASy Swiss-Prot
Match: UBE2B_BOVIN (Ubiquitin-conjugating enzyme E2 B OS=Bos taurus GN=UBE2B PE=2 SV=1)

HSP 1 Score: 169.859 bits (429), Expect = 8.323e-62
Identity = 73/94 (77.66%), Postives = 88/94 (93.62%), Query Frame = 2

Query:   89 MSTPAKKRLMRDFKRLQQDPPAGISGAPQDNNIMLWNAVIFGPDDTPWDGGTFKLTLQFTEDYPNKPPTVRFVSRMFHPNIYADGSICLDILQN 370
            MSTPA++RLMRDFKRLQ+DPP G+SGAP +NNIM WNAVIFGP+ TP++ GTFKL ++F+E+YPNKPPTVRF+S+MFHPN+YADGSICLDILQN
Sbjct:    1 MSTPARRRLMRDFKRLQEDPPVGVSGAPSENNIMQWNAVIFGPEGTPFEDGTFKLVIEFSEEYPNKPPTVRFLSKMFHPNVYADGSICLDILQN 94

HSP 2 Score: 89.7373 bits (221), Expect = 8.323e-62
Identity = 41/54 (75.93%), Postives = 48/54 (88.89%), Query Frame = 3

Query:  375 WSPIYDVAAILTSIQSLLCDPNPNSPANSEAARLFSENKREYNRRVREIVEQSW 536
            WSP YDV++ILTSIQSLL +PNPNSPANS+AA+L+ ENKREY +RV  IVEQSW
Sbjct:   96 WSPTYDVSSILTSIQSLLDEPNPNSPANSQAAQLYQENKREYEKRVSAIVEQSW 149

[Close]

BLAST of EY675113 vs. ExPASy Swiss-Prot
Match: UBC1_CAEEL (Ubiquitin-conjugating enzyme E2 1 OS=Caenorhabditis elegans GN=ubc-1 PE=1 SV=1)

HSP 1 Score: 164.851 bits (416), Expect = 1.176e-60
Identity = 68/94 (72.34%), Postives = 89/94 (94.68%), Query Frame = 2

Query:   89 MSTPAKKRLMRDFKRLQQDPPAGISGAPQDNNIMLWNAVIFGPDDTPWDGGTFKLTLQFTEDYPNKPPTVRFVSRMFHPNIYADGSICLDILQN 370
            M+TP+++RLMRDFK+LQ+DPPAG+SGAP ++NI+ W A+IFGP +TP++ GTFKL+L+FTE+YPNKPPTV+F+S+MFHPN+YADGSICLDILQN
Sbjct:    1 MTTPSRRRLMRDFKKLQEDPPAGVSGAPTEDNILTWEAIIFGPQETPFEDGTFKLSLEFTEEYPNKPPTVKFISKMFHPNVYADGSICLDILQN 94

HSP 2 Score: 90.8929 bits (224), Expect = 1.176e-60
Identity = 42/54 (77.78%), Postives = 49/54 (90.74%), Query Frame = 3

Query:  375 WSPIYDVAAILTSIQSLLCDPNPNSPANSEAARLFSENKREYNRRVREIVEQSW 536
            WSP YDVAAILTSIQSLL +PNPNSPANS AA+L+ EN+REY +RV++IVEQSW
Sbjct:   96 WSPTYDVAAILTSIQSLLDEPNPNSPANSLAAQLYQENRREYEKRVQQIVEQSW 149

[Close]

BLAST of EY675113 vs. ExPASy Swiss-Prot
Match: UBC2_USTMA (Ubiquitin-conjugating enzyme E2 2 OS=Ustilago maydis GN=UBC2 PE=3 SV=1)

HSP 1 Score: 156.762 bits (395), Expect = 1.981e-57
Identity = 69/94 (73.40%), Postives = 82/94 (87.23%), Query Frame = 2

Query:   89 MSTPAKKRLMRDFKRLQQDPPAGISGAPQDNNIMLWNAVIFGPDDTPWDGGTFKLTLQFTEDYPNKPPTVRFVSRMFHPNIYADGSICLDILQN 370
            MSTP+KKRL+RDFKRL  DPP GISGAP  +N+M+WNAVIFGP DTP++ GTFKL L F E YPNKPPTV+F+S+MFHPN+YA+G +CLDILQN
Sbjct:    1 MSTPSKKRLIRDFKRLSTDPPGGISGAPCADNLMIWNAVIFGPADTPFEDGTFKLVLTFDESYPNKPPTVKFLSKMFHPNVYANGELCLDILQN 94

HSP 2 Score: 88.1965 bits (217), Expect = 1.981e-57
Identity = 44/63 (69.84%), Postives = 49/63 (77.78%), Query Frame = 3

Query:  375 WSPIYDVAAILTSIQSLLCDPNPNSPANSEAARLFSENKREYNRRVREIVEQSWTAD*KSHES 563
            WSP YDVAAILTSIQSLL DPNPNSPAN+EAA L+ EN +EY RRV+  VE SW  D +  ES
Sbjct:   96 WSPTYDVAAILTSIQSLLHDPNPNSPANAEAASLYRENMKEYVRRVKATVEASWLDDGEMPES 158

[Close]

BLAST of EY675113 vs. ExPASy Swiss-Prot
Match: UBC2_SCHPO (Ubiquitin-conjugating enzyme E2 2 OS=Schizosaccharomyces pombe GN=rhp6 PE=1 SV=3)

HSP 1 Score: 153.295 bits (386), Expect = 6.807e-55
Identity = 67/94 (71.28%), Postives = 80/94 (85.11%), Query Frame = 2

Query:   89 MSTPAKKRLMRDFKRLQQDPPAGISGAPQDNNIMLWNAVIFGPDDTPWDGGTFKLTLQFTEDYPNKPPTVRFVSRMFHPNIYADGSICLDILQN 370
            MST A++RLMRDFKR+QQDPPAG+S +P  +N+MLWNAVI GP DTP++ GTFKL L F E YPNKPP V+FVS MFHPN+YA+G +CLDILQN
Sbjct:    1 MSTTARRRLMRDFKRMQQDPPAGVSASPVSDNVMLWNAVIIGPADTPFEDGTFKLVLSFDEQYPNKPPLVKFVSTMFHPNVYANGELCLDILQN 94

HSP 2 Score: 83.1889 bits (204), Expect = 6.807e-55
Identity = 41/54 (75.93%), Postives = 45/54 (83.33%), Query Frame = 3

Query:  375 WSPIYDVAAILTSIQSLLCDPNPNSPANSEAARLFSENKREYNRRVREIVEQSW 536
            WSP YDVAAILTSIQSLL DPN  SPAN+EAA+L  ENK+EY RRVR+ VE SW
Sbjct:   96 WSPTYDVAAILTSIQSLLNDPNNASPANAEAAQLHRENKKEYVRRVRKTVEDSW 149

[Close]

BLAST of EY675113 vs. ExPASy Swiss-Prot
Match: UBC2_CRYNE (Ubiquitin-conjugating enzyme E2 2 OS=Cryptococcus neoformans GN=UBC2 PE=3 SV=1)

HSP 1 Score: 151.754 bits (382), Expect = 1.504e-54
Identity = 67/94 (71.28%), Postives = 82/94 (87.23%), Query Frame = 2

Query:   89 MSTPAKKRLMRDFKRLQQDPPAGISGAPQDNNIMLWNAVIFGPDDTPWDGGTFKLTLQFTEDYPNKPPTVRFVSRMFHPNIYADGSICLDILQN 370
            MST AK+RL+RDFKRL  D P GISG+P  +NIM+WNAVIFGP +TP++ G+F+LTL FT+ YPNKPPTVRF+S+MFHPNIYA+G +CLDILQN
Sbjct:    1 MSTAAKRRLIRDFKRLTSDAPIGISGSPNPDNIMVWNAVIFGPPETPFEDGSFRLTLTFTDAYPNKPPTVRFISKMFHPNIYANGELCLDILQN 94

HSP 2 Score: 83.5741 bits (205), Expect = 1.504e-54
Identity = 39/54 (72.22%), Postives = 45/54 (83.33%), Query Frame = 3

Query:  375 WSPIYDVAAILTSIQSLLCDPNPNSPANSEAARLFSENKREYNRRVREIVEQSW 536
            WSP YDVAAILTS+QSLL DPNP SPAN +AA+LF EN +EY RRV++ VE SW
Sbjct:   96 WSPTYDVAAILTSVQSLLNDPNPASPANVDAAQLFKENLKEYERRVKKTVELSW 149

[Close]

BLAST of EY675113 vs. ExPASy Swiss-Prot
Match: UBC2_TRIHA (Ubiquitin-conjugating enzyme E2 2 OS=Trichoderma harzianum GN=UBC2 PE=2 SV=1)

HSP 1 Score: 148.673 bits (374), Expect = 2.138e-53
Identity = 63/94 (67.02%), Postives = 80/94 (85.11%), Query Frame = 2

Query:   89 MSTPAKKRLMRDFKRLQQDPPAGISGAPQDNNIMLWNAVIFGPDDTPWDGGTFKLTLQFTEDYPNKPPTVRFVSRMFHPNIYADGSICLDILQN 370
            MST A++RLMRDFKR+Q DPPAG+S +P  +N+M WNAVI GP DTP++ GTF+L +QF E YPNKPP V+F+S+MFHPN+YA+G +CLDILQN
Sbjct:    1 MSTAARRRLMRDFKRMQTDPPAGVSASPIPDNVMTWNAVIIGPADTPFEDGTFRLVMQFEEQYPNKPPQVKFISQMFHPNVYANGELCLDILQN 94

HSP 2 Score: 82.8037 bits (203), Expect = 2.138e-53
Identity = 38/54 (70.37%), Postives = 45/54 (83.33%), Query Frame = 3

Query:  375 WSPIYDVAAILTSIQSLLCDPNPNSPANSEAARLFSENKREYNRRVREIVEQSW 536
            WSP YDVAA+LTSIQSLL DPN  SPAN EA+ L+ +N+REY +RVRE VE+SW
Sbjct:   96 WSPTYDVAAVLTSIQSLLNDPNTGSPANVEASNLYKDNRREYIKRVRETVERSW 149

[Close]

BLAST of EY675113 vs. ExPASy Swiss-Prot
Match: UBC2_NECHA (Ubiquitin-conjugating enzyme E2 2 OS=Nectria haematococca GN=UBC2 PE=3 SV=1)

HSP 1 Score: 147.902 bits (372), Expect = 3.634e-53
Identity = 63/94 (67.02%), Postives = 78/94 (82.98%), Query Frame = 2

Query:   89 MSTPAKKRLMRDFKRLQQDPPAGISGAPQDNNIMLWNAVIFGPDDTPWDGGTFKLTLQFTEDYPNKPPTVRFVSRMFHPNIYADGSICLDILQN 370
            MST A++RLMRDFKR+Q DPPAG+S +P  +N+M WNAVI GP DTP++ GTF+L +QF E YPNKPP V+F+S MFHPN+YA G +CLDILQN
Sbjct:    1 MSTAARRRLMRDFKRMQTDPPAGVSASPVPDNVMTWNAVIIGPADTPFEDGTFRLVMQFEEQYPNKPPQVKFISEMFHPNVYATGELCLDILQN 94

HSP 2 Score: 82.8037 bits (203), Expect = 3.634e-53
Identity = 37/54 (68.52%), Postives = 45/54 (83.33%), Query Frame = 3

Query:  375 WSPIYDVAAILTSIQSLLCDPNPNSPANSEAARLFSENKREYNRRVREIVEQSW 536
            WSP YDVAA+LTSIQSLL DPN  SPAN EA+ L+ +N++EY +RVRE VE+SW
Sbjct:   96 WSPTYDVAAVLTSIQSLLNDPNTGSPANVEASNLYKDNRKEYTKRVRETVEKSW 149

The following BLAST results are available for this feature:

BLAST of EY675113 vs. ExPASy Swiss-Prot

BLAST of EY675113 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 228


Match Name	E-value	Identity	Description
UBE2B_RABIT	8.323e-62	77.66	Ubiquitin-conjugating enzyme E2 B OS=Oryctolagus c...	[more]
UBE2B_MOUSE	8.323e-62	77.66	Ubiquitin-conjugating enzyme E2 B OS=Mus musculus ...	[more]
UBE2B_HUMAN	8.323e-62	77.66	Ubiquitin-conjugating enzyme E2 B OS=Homo sapiens ...	[more]
UBE2B_BOVIN	8.323e-62	77.66	Ubiquitin-conjugating enzyme E2 B OS=Bos taurus GN...	[more]
UBC1_CAEEL	1.176e-60	72.34	Ubiquitin-conjugating enzyme E2 1 OS=Caenorhabditi...	[more]
UBC2_USTMA	1.981e-57	73.40	Ubiquitin-conjugating enzyme E2 2 OS=Ustilago mayd...	[more]
UBC2_SCHPO	6.807e-55	71.28	Ubiquitin-conjugating enzyme E2 2 OS=Schizosacchar...	[more]
UBC2_CRYNE	1.504e-54	71.28	Ubiquitin-conjugating enzyme E2 2 OS=Cryptococcus ...	[more]
UBC2_TRIHA	2.138e-53	67.02	Ubiquitin-conjugating enzyme E2 2 OS=Trichoderma h...	[more]
UBC2_NECHA	3.634e-53	67.02	Ubiquitin-conjugating enzyme E2 2 OS=Nectria haema...	[more]

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Properties


Property Name	Value
Genbank description	CS00-C1-401-006-C04-CT.F Sweet orange leaf, infected with CiLV Citrus sinensis cDNA, mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>EY675113 ID=EY675113; Name=EY675113; organism=Citrus sinensis; type=EST; length=988bp
TTCTTTCTCTAGAAGCTTCTCCTCTCATTCTCCCCCCTTACCCCCTCTGC
AAAAGCCGCTTCTCAGTTTTGCTCTCTAAAACGCCACTATGTCAACACCG
GCGAAGAAGCGGCTGATGCGGGACTTCAAGCGCCTCCAGCAAGATCCACC
CGCCGGAATCAGCGGGGCCCCTCAAGACAACAACATCATGTTATGGAACG
CCGTTATTTTTGGGCCTGATGATACTCCCTGGGACGGAGGTACGTTCAAG
TTGACTCTTCAGTTTACAGAGGATTATCCGAATAAACCTCCAACAGTTCG
GTTCGTTTCACGGATGTTTCATCCAAATATCTATGCGGATGGAAGTATCT
GCCTGGATATATTACAAAATCANGTGGAGTCCTATATATGATGTTGCAGC
TATACTGACCTCCATCCAGTCGCTGCTTTGTGATCCAAATCCAAACTCCC
CTGCAAATTCTGAAGCAGCTCGGCTGTTTAGTGAGAATAAACGAGAGTAC
AACCGAAGAGTGCGTGAAATAGTGGAGCAAAGCTGGACAGCCGACTAAAA
GTCCCATGAAAGCACCGGCCGGTTATGCTGGAGAAATAAAAGATGAAAAC
TGGGGCTGGTGGGGTTGGTTTGGTCAAAATAATGGAACCCGTATTCAATC
GGATTTCAGGTGCATTGTTAATTAAAAAAATCCCCTTCGAATCTTTCCTC
CCCAAAAACCCTGGGTGGAACCCTTGGTTATTTTTCCAACATTTCCCTCT
TCCTTTTCCAAAACTTTCCGGGTAAATGTAATTTTATTGGGGACATTTTG
GTAAGGGGGGTTTTTTGGGGGGCGGGAAAACCCTGTGGATGTTGTTCTTT
AAAAAAACCCCCCCAAAAAAGAAACCCCTTTTTTTTTTTGGGAAATTTTT
TTTATGGGGGGATATTTTTTTGGGGGGATTTGTGGTGGGCGGGGGGGCGC
CCAAAAAAAAATAAAAAAAAAAAAAGGAAACAAAAACA