EY675113

Overview
NameEY675113
Unique NameEY675113
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length988
Libraries
Library NameType
Sweet orange leaf, infected with CiLVcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of EY675113 vs. ExPASy Swiss-Prot
Match: UBC9_MESAU (SUMO-conjugating enzyme UBC9 OS=Mesocricetus auratus GN=UBE2I PE=2 SV=1)

HSP 1 Score: 87.0409 bits (214), Expect = 2.169e-22
Identity = 41/98 (41.84%), Postives = 56/98 (57.14%), Query Frame = 2 
Query:   89 MSTPAKKRLMRDFKRLQQDPPAGISGAPQDN-----NIMLWNAVIFGPDDTPWDGGTFKLTLQFTEDYPNKPPTVRFVSRMFHPNIYADGSICLDILQ 367
            MS  A  RL ++ K  ++D P G    P  N     N+M W   I G   TPW+GG FKL + F +DYP+ PP  +F   +FHPN+Y  G++CL IL+
Sbjct:    1 MSGIALSRLAQERKAWRKDHPFGFVAVPTKNPDGTMNLMNWECAIPGKKGTPWEGGLFKLRMLFKDDYPSSPPKCKFEPPLFHPNVYPSGTVCLSILE 98

HSP 2 Score: 40.4318 bits (93), Expect = 2.169e-22
Identity = 17/47 (36.17%), Postives = 26/47 (55.32%), Query Frame = 3 
Query:  375 WSPIYDVAAILTSIQSLLCDPNPNSPANSEAARLFSENKREYNRRVR 515
            W P   +  +   IQ LL +PN   PA +EA  ++ +N+ EY +R R
Sbjct:  103 WRPAITINQLFIGIQELLNEPNIQEPAQAEAYTIYCQNRVEYEKRFR 149
[Close]
BLAST of EY675113 vs. ExPASy Swiss-Prot
Match: UB2G1_RAT (Ubiquitin-conjugating enzyme E2 G1 OS=Rattus norvegicus GN=Ube2g1 PE=2 SV=3)

HSP 1 Score: 79.7221 bits (195), Expect = 2.812e-22
Identity = 35/90 (38.89%), Postives = 54/90 (60.00%), Query Frame = 2 
Query:  113 LMRDFKRLQQDPPAGIS-GAPQDNNIMLWNAVIFGPDDTPWDGGTFKLTLQFTEDYPNKPPTVRFVSRMFHPNIYADGSICLDILQNXVE 379
            L R    L ++P  G S G   DN++  W  +I GP DT ++GG FK  L F +DYP +PP ++F++ ++HPN+  +G +C+ IL    E
Sbjct:   10 LRRQLAELNKNPVEGFSAGLIDDNDLYRWEVLIIGPPDTLYEGGVFKAHLTFPKDYPLRPPKMKFITEIWHPNVDKNGDVCISILHEPGE 99

HSP 2 Score: 47.3654 bits (111), Expect = 2.812e-22
Identity = 23/54 (42.59%), Postives = 36/54 (66.67%), Query Frame = 3 
Query:  375 WSPIYDVAAILTSIQSLLCDPNPNSPANSEAARLFSENKR-EYNRRVREIVEQS 533
            W PI+ V  I+ S+ S+L DPN +SPAN +AA+ + E++  E+ R+V   V +S
Sbjct:  111 WLPIHTVETIMISVISMLADPNGDSPANVDAAKEWREDRNGEFKRKVARCVRKS 164
[Close]
BLAST of EY675113 vs. ExPASy Swiss-Prot
Match: UB2G1_MOUSE (Ubiquitin-conjugating enzyme E2 G1 OS=Mus musculus GN=Ube2g1 PE=2 SV=3)

HSP 1 Score: 79.7221 bits (195), Expect = 2.812e-22
Identity = 35/90 (38.89%), Postives = 54/90 (60.00%), Query Frame = 2 
Query:  113 LMRDFKRLQQDPPAGIS-GAPQDNNIMLWNAVIFGPDDTPWDGGTFKLTLQFTEDYPNKPPTVRFVSRMFHPNIYADGSICLDILQNXVE 379
            L R    L ++P  G S G   DN++  W  +I GP DT ++GG FK  L F +DYP +PP ++F++ ++HPN+  +G +C+ IL    E
Sbjct:   10 LRRQLAELNKNPVEGFSAGLIDDNDLYRWEVLIIGPPDTLYEGGVFKAHLTFPKDYPLRPPKMKFITEIWHPNVDKNGDVCISILHEPGE 99

HSP 2 Score: 47.3654 bits (111), Expect = 2.812e-22
Identity = 23/54 (42.59%), Postives = 36/54 (66.67%), Query Frame = 3 
Query:  375 WSPIYDVAAILTSIQSLLCDPNPNSPANSEAARLFSENKR-EYNRRVREIVEQS 533
            W PI+ V  I+ S+ S+L DPN +SPAN +AA+ + E++  E+ R+V   V +S
Sbjct:  111 WLPIHTVETIMISVISMLADPNGDSPANVDAAKEWREDRNGEFKRKVARCVRKS 164
[Close]
BLAST of EY675113 vs. ExPASy Swiss-Prot
Match: UB2G1_MACFA (Ubiquitin-conjugating enzyme E2 G1 OS=Macaca fascicularis GN=UBE2G1 PE=2 SV=1)

HSP 1 Score: 79.7221 bits (195), Expect = 2.812e-22
Identity = 35/90 (38.89%), Postives = 54/90 (60.00%), Query Frame = 2 
Query:  113 LMRDFKRLQQDPPAGIS-GAPQDNNIMLWNAVIFGPDDTPWDGGTFKLTLQFTEDYPNKPPTVRFVSRMFHPNIYADGSICLDILQNXVE 379
            L R    L ++P  G S G   DN++  W  +I GP DT ++GG FK  L F +DYP +PP ++F++ ++HPN+  +G +C+ IL    E
Sbjct:   10 LRRQLAELNKNPVEGFSAGLIDDNDLYRWEVLIIGPPDTLYEGGVFKAHLTFPKDYPLRPPKMKFITEIWHPNVDKNGDVCISILHEPGE 99

HSP 2 Score: 47.3654 bits (111), Expect = 2.812e-22
Identity = 23/54 (42.59%), Postives = 36/54 (66.67%), Query Frame = 3 
Query:  375 WSPIYDVAAILTSIQSLLCDPNPNSPANSEAARLFSENKR-EYNRRVREIVEQS 533
            W PI+ V  I+ S+ S+L DPN +SPAN +AA+ + E++  E+ R+V   V +S
Sbjct:  111 WLPIHTVETIMISVISMLADPNGDSPANVDAAKEWREDRNGEFKRKVARCVRKS 164
[Close]
BLAST of EY675113 vs. ExPASy Swiss-Prot
Match: UB2G1_HUMAN (Ubiquitin-conjugating enzyme E2 G1 OS=Homo sapiens GN=UBE2G1 PE=1 SV=3)

HSP 1 Score: 79.7221 bits (195), Expect = 2.812e-22
Identity = 35/90 (38.89%), Postives = 54/90 (60.00%), Query Frame = 2 
Query:  113 LMRDFKRLQQDPPAGIS-GAPQDNNIMLWNAVIFGPDDTPWDGGTFKLTLQFTEDYPNKPPTVRFVSRMFHPNIYADGSICLDILQNXVE 379
            L R    L ++P  G S G   DN++  W  +I GP DT ++GG FK  L F +DYP +PP ++F++ ++HPN+  +G +C+ IL    E
Sbjct:   10 LRRQLAELNKNPVEGFSAGLIDDNDLYRWEVLIIGPPDTLYEGGVFKAHLTFPKDYPLRPPKMKFITEIWHPNVDKNGDVCISILHEPGE 99

HSP 2 Score: 47.3654 bits (111), Expect = 2.812e-22
Identity = 23/54 (42.59%), Postives = 36/54 (66.67%), Query Frame = 3 
Query:  375 WSPIYDVAAILTSIQSLLCDPNPNSPANSEAARLFSENKR-EYNRRVREIVEQS 533
            W PI+ V  I+ S+ S+L DPN +SPAN +AA+ + E++  E+ R+V   V +S
Sbjct:  111 WLPIHTVETIMISVISMLADPNGDSPANVDAAKEWREDRNGEFKRKVARCVRKS 164
[Close]
BLAST of EY675113 vs. ExPASy Swiss-Prot
Match: UBE2N_MACFA (Ubiquitin-conjugating enzyme E2 N OS=Macaca fascicularis GN=UBE2N PE=2 SV=1)

HSP 1 Score: 89.3521 bits (220), Expect = 2.829e-22
Identity = 38/88 (43.18%), Postives = 61/88 (69.32%), Query Frame = 2 
Query:  107 KRLMRDFKRLQQDPPAGISGAPQDNNIMLWNAVIFGPDDTPWDGGTFKLTLQFTEDYPNKPPTVRFVSRMFHPNIYADGSICLDILQN 370
            +R++++ +RL  +P  GI   P ++N   ++ VI GP D+P++GGTFKL L   E+YP   P VRF+++++HPN+   G ICLDIL++
Sbjct:    6 RRIIKETQRLLAEPVPGIKAEPDESNARYFHVVIAGPQDSPFEGGTFKLELFLPEEYPMAAPKVRFMTKIYHPNVDKSGRICLDILKD 93

HSP 2 Score: 37.7354 bits (86), Expect = 2.829e-22
Identity = 16/44 (36.36%), Postives = 25/44 (56.82%), Query Frame = 3 
Query:  366 KIXWSPIYDVAAILTSIQSLLCDPNPNSPANSEAARLFSENKRE 497
            K  WSP   +  +L SIQ+LL  PNP+ P  ++ A  +  N+ +
Sbjct:   92 KDKWSPALQIRTVLLSIQALLSAPNPDDPLANDVAEQWKTNEAQ 135
[Close]
BLAST of EY675113 vs. ExPASy Swiss-Prot
Match: UBE2C_DROME (Ubiquitin-conjugating enzyme E2 C OS=Drosophila melanogaster GN=vih PE=1 SV=1)

HSP 1 Score: 77.411 bits (189), Expect = 4.750e-22
Identity = 36/88 (40.91%), Postives = 50/88 (56.82%), Query Frame = 2 
Query:  107 KRLMRDFKRLQQDPPAGISGAPQDNNIMLWNAVIFGPDDTPWDGGTFKLTLQFTEDYPNKPPTVRFVSRMFHPNIYADGSICLDILQN 370
            KRL ++   L      GIS  P   NI  W   I GP +T + G T++L+L F   YP   P V+F++  FHPN+   G+ICLDIL++
Sbjct:   35 KRLHKELMNLMMANERGISAFPDGENIFKWVGTIAGPRNTVYSGQTYRLSLDFPNSYPYAAPVVKFLTSCFHPNVDLQGAICLDILKD 122

HSP 2 Score: 48.9062 bits (115), Expect = 4.750e-22
Identity = 25/55 (45.45%), Postives = 36/55 (65.45%), Query Frame = 3 
Query:  366 KIXWSPIYDVAAILTSIQSLLCDPNPNSPANSEAARLFSENKREYNRRVREIVEQ 530
            K  WS +YDV  IL SIQSLL +PN  SP N++AA ++++ K EY + +    E+
Sbjct:  121 KDKWSALYDVRTILLSIQSLLGEPNNESPLNAQAAMMWNDQK-EYKKYLDAFYEK 174
[Close]
BLAST of EY675113 vs. ExPASy Swiss-Prot
Match: UBE2N_RAT (Ubiquitin-conjugating enzyme E2 N OS=Rattus norvegicus GN=Ube2n PE=1 SV=1)

HSP 1 Score: 87.8113 bits (216), Expect = 6.232e-22
Identity = 38/88 (43.18%), Postives = 61/88 (69.32%), Query Frame = 2 
Query:  107 KRLMRDFKRLQQDPPAGISGAPQDNNIMLWNAVIFGPDDTPWDGGTFKLTLQFTEDYPNKPPTVRFVSRMFHPNIYADGSICLDILQN 370
            +R++++ +RL  +P  GI   P ++N   ++ VI GP D+P++GGTFKL L   E+YP   P VRF+++++HPN+   G ICLDIL++
Sbjct:    6 RRIIKETQRLLAEPVPGIKAEPDESNARYFHVVIAGPQDSPFEGGTFKLELFLPEEYPMAAPKVRFMTKIYHPNVDKLGRICLDILKD 93

HSP 2 Score: 38.1206 bits (87), Expect = 6.232e-22
Identity = 16/44 (36.36%), Postives = 25/44 (56.82%), Query Frame = 3 
Query:  366 KIXWSPIYDVAAILTSIQSLLCDPNPNSPANSEAARLFSENKRE 497
            K  WSP   +  +L SIQ+LL  PNP+ P  ++ A  +  N+ +
Sbjct:   92 KDKWSPALQIRTVLLSIQALLSAPNPDDPLANDVAEQWKSNEAQ 135
[Close]
BLAST of EY675113 vs. ExPASy Swiss-Prot
Match: UBC_ASFB7 (Ubiquitin-conjugating enzyme E2 OS=African swine fever virus (strain Badajoz 1971 Vero-adapted) GN=UBC PE=2 SV=1)

HSP 1 Score: 80.1073 bits (196), Expect = 7.987e-22
Identity = 33/84 (39.29%), Postives = 52/84 (61.90%), Query Frame = 2 
Query:  113 LMRDFKRLQQDPPAGISGAPQDNNIMLWNAVIFGPDDTPWDGGTFKLTLQFTEDYPNKPPTVRFVSRMFHPNIYADGSICLDIL 364
            L+ +++ L ++P      +  +NNI  W+ ++ GP DT ++GG FK  + F  +YP  PP + F S M+HPNIY DG +C+ IL
Sbjct:    6 LIAEYRHLIENPSENFKISVNENNITEWDVILRGPPDTLYEGGLFKAKVAFPPEYPYAPPKLTFTSEMWHPNIYPDGRLCISIL 89

HSP 2 Score: 45.4394 bits (106), Expect = 7.987e-22
Identity = 23/58 (39.66%), Postives = 36/58 (62.07%), Query Frame = 3 
Query:  375 WSPIYDVAAILTSIQSLLCDPNPNSPANSEAAR-----LFSENKREYNRRVREIVEQS 533
            WSP   +  IL S+ SLL +PNP+SPAN +AA+     ++ E+   Y   V++ V++S
Sbjct:  101 WSPAQKIDTILLSVISLLNEPNPDSPANVDAAKSYRKYVYKEDLESYPMEVKKTVKKS 158
[Close]
BLAST of EY675113 vs. ExPASy Swiss-Prot
Match: UBC9_YEAST (SUMO-conjugating enzyme UBC9 OS=Saccharomyces cerevisiae GN=UBC9 PE=1 SV=1)

HSP 1 Score: 76.6406 bits (187), Expect = 8.090e-22
Identity = 35/97 (36.08%), Postives = 56/97 (57.73%), Query Frame = 2 
Query:   89 MSTPAKKRLMRDFKRLQQDPPAGISGAPQDN-----NIMLWNAVIFGPDDTPWDGGTFKLTLQFTEDYPNKPPTVRFVSRMFHPNIYADGSICLDIL 364
            MS+   +RL  + K+ ++D P G    P        ++  W A I G + T W GG + +T+++  +YP+KPP V+F +  +HPN+Y  G+ICL IL
Sbjct:    1 MSSLCLQRLQEERKKWRKDHPFGFYAKPVKKADGSMDLQKWEAGIPGKEGTNWAGGVYPITVEYPNEYPSKPPKVKFPAGFYHPNVYPSGTICLSIL 97

HSP 2 Score: 48.9062 bits (115), Expect = 8.090e-22
Identity = 22/46 (47.83%), Postives = 28/46 (60.87%), Query Frame = 3 
Query:  375 WSPIYDVAAILTSIQSLLCDPNPNSPANSEAARLFSENKREYNRRV 512
            W P   +  I+  +Q LL  PNPNSPA   A R FS NK EY+++V
Sbjct:  103 WRPAITLKQIVLGVQDLLDSPNPNSPAQEPAWRSFSRNKAEYDKKV 148
The following BLAST results are available for this feature:
BLAST of EY675113 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 228
Match NameE-valueIdentityDescription
UBC9_MESAU2.169e-2241.84SUMO-conjugating enzyme UBC9 OS=Mesocricetus aurat... [more]
UB2G1_RAT2.812e-2238.89Ubiquitin-conjugating enzyme E2 G1 OS=Rattus norve... [more]
UB2G1_MOUSE2.812e-2238.89Ubiquitin-conjugating enzyme E2 G1 OS=Mus musculus... [more]
UB2G1_MACFA2.812e-2238.89Ubiquitin-conjugating enzyme E2 G1 OS=Macaca fasci... [more]
UB2G1_HUMAN2.812e-2238.89Ubiquitin-conjugating enzyme E2 G1 OS=Homo sapiens... [more]
UBE2N_MACFA2.829e-2243.18Ubiquitin-conjugating enzyme E2 N OS=Macaca fascic... [more]
UBE2C_DROME4.750e-2240.91Ubiquitin-conjugating enzyme E2 C OS=Drosophila me... [more]
UBE2N_RAT6.232e-2243.18Ubiquitin-conjugating enzyme E2 N OS=Rattus norveg... [more]
UBC_ASFB77.987e-2239.29Ubiquitin-conjugating enzyme E2 OS=African swine f... [more]
UBC9_YEAST8.090e-2236.08SUMO-conjugating enzyme UBC9 OS=Saccharomyces cere... [more]

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Properties
Property NameValue
Genbank descriptionCS00-C1-401-006-C04-CT.F Sweet orange leaf, infected with CiLV Citrus sinensis cDNA, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>EY675113 ID=EY675113; Name=EY675113; organism=Citrus sinensis; type=EST; length=988bp
TTCTTTCTCTAGAAGCTTCTCCTCTCATTCTCCCCCCTTACCCCCTCTGC
AAAAGCCGCTTCTCAGTTTTGCTCTCTAAAACGCCACTATGTCAACACCG
GCGAAGAAGCGGCTGATGCGGGACTTCAAGCGCCTCCAGCAAGATCCACC
CGCCGGAATCAGCGGGGCCCCTCAAGACAACAACATCATGTTATGGAACG
CCGTTATTTTTGGGCCTGATGATACTCCCTGGGACGGAGGTACGTTCAAG
TTGACTCTTCAGTTTACAGAGGATTATCCGAATAAACCTCCAACAGTTCG
GTTCGTTTCACGGATGTTTCATCCAAATATCTATGCGGATGGAAGTATCT
GCCTGGATATATTACAAAATCANGTGGAGTCCTATATATGATGTTGCAGC
TATACTGACCTCCATCCAGTCGCTGCTTTGTGATCCAAATCCAAACTCCC
CTGCAAATTCTGAAGCAGCTCGGCTGTTTAGTGAGAATAAACGAGAGTAC
AACCGAAGAGTGCGTGAAATAGTGGAGCAAAGCTGGACAGCCGACTAAAA
GTCCCATGAAAGCACCGGCCGGTTATGCTGGAGAAATAAAAGATGAAAAC
TGGGGCTGGTGGGGTTGGTTTGGTCAAAATAATGGAACCCGTATTCAATC
GGATTTCAGGTGCATTGTTAATTAAAAAAATCCCCTTCGAATCTTTCCTC
CCCAAAAACCCTGGGTGGAACCCTTGGTTATTTTTCCAACATTTCCCTCT
TCCTTTTCCAAAACTTTCCGGGTAAATGTAATTTTATTGGGGACATTTTG
GTAAGGGGGGTTTTTTGGGGGGCGGGAAAACCCTGTGGATGTTGTTCTTT
AAAAAAACCCCCCCAAAAAAGAAACCCCTTTTTTTTTTTGGGAAATTTTT
TTTATGGGGGGATATTTTTTTGGGGGGATTTGTGGTGGGCGGGGGGGCGC
CCAAAAAAAAATAAAAAAAAAAAAAGGAAACAAAAACA
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