EY675113

Overview
NameEY675113
Unique NameEY675113
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length988
Libraries
Library NameType
Sweet orange leaf, infected with CiLVcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of EY675113 vs. ExPASy Swiss-Prot
Match: UBC1_SCHPO (Ubiquitin-conjugating enzyme E2 1 OS=Schizosaccharomyces pombe GN=ubc1 PE=2 SV=2)

HSP 1 Score: 70.0922 bits (170), Expect = 8.522e-21
Identity = 33/95 (34.74%), Postives = 59/95 (62.11%), Query Frame = 2 
Query:   89 MSTPAKKRLMRDFKRLQQDPPAGISGAPQDNNIMLWNAVIFGPDDTPWDGGTFKLTLQFTEDYPNKPPTVRFVSRMFHPNIYAD-GSICLDILQN 370
            MS    +R+ ++   +QQD  AGI     +++I     +  GP+ TP++GG F + ++   DYP +PP + F ++++HPN+ +  G+ICLDIL++
Sbjct:    1 MSDNRSRRIAKELADVQQDKQAGIQVWTINDDISHLKGMFRGPEGTPYEGGYFVVDIEIPIDYPFRPPKMNFDTKIYHPNVSSQTGAICLDILKD 95

HSP 2 Score: 51.9878 bits (123), Expect = 8.522e-21
Identity = 22/59 (37.29%), Postives = 39/59 (66.10%), Query Frame = 3 
Query:  366 KIXWSPIYDVAAILTSIQSLLCDPNPNSPANSEAARLFSENKREYNRRVREIVEQSWTA 542
            K  WSP+Y + + L S+QSLLC P P++P +++ A+++ +N +++ R  RE     WT+
Sbjct:   94 KDQWSPVYTMKSALISLQSLLCTPEPSNPQDAQVAQVYLQNYQQFVRTARE-----WTS 147
[Close]
BLAST of EY675113 vs. ExPASy Swiss-Prot
Match: UBE2C_MACFA (Ubiquitin-conjugating enzyme E2 C OS=Macaca fascicularis GN=UBE2C PE=2 SV=1)

HSP 1 Score: 75.0998 bits (183), Expect = 1.117e-20
Identity = 34/91 (37.36%), Postives = 52/91 (57.14%), Query Frame = 2 
Query:   98 PAKKRLMRDFKRLQQDPPAGISGAPQDNNIMLWNAVIFGPDDTPWDGGTFKLTLQFTEDYPNKPPTVRFVSRMFHPNIYADGSICLDILQN 370
            P  KRL ++   L      GIS  P+ +N+  W   I G   T ++   +KL+L+F   YP   PTV+F++  +HPN+   G+ICLDIL++
Sbjct:   30 PVGKRLQQELMTLMMSGDKGISAFPESDNLFKWVGTIHGAAGTVYEDLRYKLSLEFPSGYPYNAPTVKFLTPCYHPNVDTQGNICLDILKD 120

HSP 2 Score: 46.595 bits (109), Expect = 1.117e-20
Identity = 25/59 (42.37%), Postives = 37/59 (62.71%), Query Frame = 3 
Query:  366 KIXWSPIYDVAAILTSIQSLLCDPNPNSPANSEAARLFSENKREYNRRVREIVEQSWTA 542
            K  WS +YDV  IL SIQSLL +PN +SP N+ AA L+ +N   + + ++E   +  T+
Sbjct:  119 KDKWSALYDVRTILLSIQSLLGEPNIDSPLNTHAAELW-KNPTAFKKYLQETYSKQVTS 176
[Close]
BLAST of EY675113 vs. ExPASy Swiss-Prot
Match: UBE2C_HUMAN (Ubiquitin-conjugating enzyme E2 C OS=Homo sapiens GN=UBE2C PE=1 SV=1)

HSP 1 Score: 74.7146 bits (182), Expect = 1.453e-20
Identity = 34/90 (37.78%), Postives = 51/90 (56.67%), Query Frame = 2 
Query:   98 PAKKRLMRDFKRLQQDPPAGISGAPQDNNIMLWNAVIFGPDDTPWDGGTFKLTLQFTEDYPNKPPTVRFVSRMFHPNIYADGSICLDILQ 367
            P  KRL ++   L      GIS  P+ +N+  W   I G   T ++   +KL+L+F   YP   PTV+F++  +HPN+   G+ICLDIL+
Sbjct:   30 PVGKRLQQELMTLMMSGDKGISAFPESDNLFKWVGTIHGAAGTVYEDLRYKLSLEFPSGYPYNAPTVKFLTPCYHPNVDTQGNICLDILK 119

HSP 2 Score: 46.595 bits (109), Expect = 1.453e-20
Identity = 25/59 (42.37%), Postives = 37/59 (62.71%), Query Frame = 3 
Query:  366 KIXWSPIYDVAAILTSIQSLLCDPNPNSPANSEAARLFSENKREYNRRVREIVEQSWTA 542
            K  WS +YDV  IL SIQSLL +PN +SP N+ AA L+ +N   + + ++E   +  T+
Sbjct:  119 KEKWSALYDVRTILLSIQSLLGEPNIDSPLNTHAAELW-KNPTAFKKYLQETYSKQVTS 176
[Close]
BLAST of EY675113 vs. ExPASy Swiss-Prot
Match: UBE2C_BOVIN (Ubiquitin-conjugating enzyme E2 C OS=Bos taurus GN=UBE2C PE=2 SV=1)

HSP 1 Score: 75.0998 bits (183), Expect = 1.890e-20
Identity = 34/91 (37.36%), Postives = 52/91 (57.14%), Query Frame = 2 
Query:   98 PAKKRLMRDFKRLQQDPPAGISGAPQDNNIMLWNAVIFGPDDTPWDGGTFKLTLQFTEDYPNKPPTVRFVSRMFHPNIYADGSICLDILQN 370
            P  KRL ++   L      GIS  P+ +N+  W   I G   T ++   +KL+L+F   YP   PTV+F++  +HPN+   G+ICLDIL++
Sbjct:   30 PVGKRLQQELMTLMMSGDKGISAFPESDNLFKWVGTIHGAAGTVYEDLRYKLSLEFPSGYPYNAPTVKFLTPCYHPNVDTQGNICLDILKD 120

HSP 2 Score: 45.8246 bits (107), Expect = 1.890e-20
Identity = 24/51 (47.06%), Postives = 34/51 (66.67%), Query Frame = 3 
Query:  366 KIXWSPIYDVAAILTSIQSLLCDPNPNSPANSEAARLFSENKREYNRRVRE 518
            K  WS +YDV  IL SIQSLL +PN +SP N+ AA L+ +N   + + ++E
Sbjct:  119 KDKWSALYDVRTILLSIQSLLGEPNIDSPLNTHAAELW-KNPTAFKKYLQE 168
[Close]
BLAST of EY675113 vs. ExPASy Swiss-Prot
Match: UBE2C_XENLA (Ubiquitin-conjugating enzyme E2 C OS=Xenopus laevis GN=ube2c PE=1 SV=1)

HSP 1 Score: 74.3294 bits (181), Expect = 1.890e-20
Identity = 35/88 (39.77%), Postives = 52/88 (59.09%), Query Frame = 2 
Query:  107 KRLMRDFKRLQQDPPAGISGAPQDNNIMLWNAVIFGPDDTPWDGGTFKLTLQFTEDYPNKPPTVRFVSRMFHPNIYADGSICLDILQN 370
            KRL ++   L      GIS  P+ +N+  W   I G   T ++   +KL+L+F   YP   PTV+FV+  FHPN+ + G+ICLDIL++
Sbjct:   33 KRLQQELMTLMMSGDKGISAFPESDNLFKWIGTIDGAVGTVYEDLRYKLSLEFPSGYPYNAPTVKFVTPCFHPNVDSHGNICLDILKD 120

HSP 2 Score: 46.595 bits (109), Expect = 1.890e-20
Identity = 24/51 (47.06%), Postives = 32/51 (62.75%), Query Frame = 3 
Query:  366 KIXWSPIYDVAAILTSIQSLLCDPNPNSPANSEAARLFSENKREYNRRVRE 518
            K  WS +YDV  IL S+QSLL +PN  SP N  AA L+ +N+  Y + + E
Sbjct:  119 KDKWSALYDVRTILLSLQSLLGEPNNESPLNPYAAELW-QNQTAYKKHLHE 168
[Close]
BLAST of EY675113 vs. ExPASy Swiss-Prot
Match: UBC_ASFK5 (Ubiquitin-conjugating enzyme E2 OS=African swine fever virus (isolate Pig/Kenya/KEN-50/1950) GN=UBC PE=2 SV=1)

HSP 1 Score: 80.8777 bits (198), Expect = 2.440e-20
Identity = 32/84 (38.10%), Postives = 53/84 (63.10%), Query Frame = 2 
Query:  113 LMRDFKRLQQDPPAGISGAPQDNNIMLWNAVIFGPDDTPWDGGTFKLTLQFTEDYPNKPPTVRFVSRMFHPNIYADGSICLDIL 364
            L+ +++ L ++P      +  +NN+  W+ ++ GP DT ++GG FK  + F  +YP +PP + F S M+HPNIY DG +C+ IL
Sbjct:    6 LLTEYRNLTENPSEHFKVSVNENNLTEWDVILKGPSDTFYEGGLFKAKITFPPNYPYEPPKLTFTSEMWHPNIYPDGRLCISIL 89

HSP 2 Score: 39.6614 bits (91), Expect = 2.440e-20
Identity = 20/58 (34.48%), Postives = 33/58 (56.90%), Query Frame = 3 
Query:  375 WSPIYDVAAILTSIQSLLCDPNPNSPANSEAAR-----LFSENKREYNRRVREIVEQS 533
            WSP   +  +L S+ SL  +PN +SPAN +AA+     +  E+   Y   V++ V++S
Sbjct:  101 WSPAQKIETVLLSVISLFNEPNTDSPANVDAAKSYRKLMHKEDLESYPMEVKKTVKKS 158
[Close]
BLAST of EY675113 vs. ExPASy Swiss-Prot
Match: UBC31_ARATH (Probable ubiquitin carrier protein E2 31 OS=Arabidopsis thaliana GN=UBC31 PE=2 SV=1)

HSP 1 Score: 79.337 bits (194), Expect = 2.477e-20
Identity = 34/64 (53.12%), Postives = 47/64 (73.44%), Query Frame = 2 
Query:  179 NNIMLWNAVIFGPDDTPWDGGTFKLTLQFTEDYPNKPPTVRFVSRMFHPNIYADGSICLDILQN 370
            NNI  W AVI GPD TP++GG F L+++F  DYP KPP   F + ++HPNI  +GSIC++IL++
Sbjct:   34 NNIYEWTAVIRGPDGTPYEGGMFNLSIKFPTDYPFKPPKFTFKTPIYHPNINDEGSICMNILKD 97

HSP 2 Score: 41.2022 bits (95), Expect = 2.477e-20
Identity = 20/51 (39.22%), Postives = 29/51 (56.86%), Query Frame = 3 
Query:  366 KIXWSPIYDVAAILTSIQSLLCDPNPNSPANSEAARLFSENKREYNRRVRE 518
            K  W+P   V  +L SI  LL  PNP+ P   E  +LF  N+ ++++R RE
Sbjct:   96 KDKWTPALMVEKVLLSILLLLEKPNPDDPLVPEIGQLFKNNRFQFDQRARE 146
[Close]
BLAST of EY675113 vs. ExPASy Swiss-Prot
Match: UB2R1_MOUSE (Ubiquitin-conjugating enzyme E2 R1 OS=Mus musculus GN=Cdc34 PE=1 SV=1)

HSP 1 Score: 82.0333 bits (201), Expect = 3.166e-20
Identity = 38/94 (40.43%), Postives = 57/94 (60.64%), Query Frame = 2 
Query:  101 AKKRLMRDFKRLQQDPPAGISGAPQDN-NIMLWNAVIFGPDDTPWDGGTFKLTLQFTEDYPNKPPTVRFVSRMFHPNIYADGSICLDILQNXVE 379
            ++K L+ + K LQ++P  G      D  ++  W   IFGP +T ++GG FK  L+F  DYP  PP  RF+++M+HPNIY  G +C+ IL   V+
Sbjct:    9 SQKALLLELKGLQEEPVEGFRVTLVDEGDLYNWEVAIFGPPNTYYEGGYFKARLKFPIDYPYSPPAFRFLTKMWHPNIYETGDVCISILHPPVD 102

HSP 2 Score: 38.1206 bits (87), Expect = 3.166e-20
Identity = 24/56 (42.86%), Postives = 34/56 (60.71%), Query Frame = 3 
Query:  375 WSPIYDVAAILTSIQSLLCDPNPNSPANSEAA---RLFSENK---REYNRRVREIV 524
            W+P  +V  IL S+ SLL +PN  SPAN +A+   R + E+K   REY   +R+ V
Sbjct:  114 WNPTQNVRTILLSVISLLNEPNTFSPANVDASVMYRKWKESKGKDREYTDIIRKQV 169
[Close]
BLAST of EY675113 vs. ExPASy Swiss-Prot
Match: UB2R1_HUMAN (Ubiquitin-conjugating enzyme E2 R1 OS=Homo sapiens GN=CDC34 PE=1 SV=2)

HSP 1 Score: 82.0333 bits (201), Expect = 3.166e-20
Identity = 38/94 (40.43%), Postives = 57/94 (60.64%), Query Frame = 2 
Query:  101 AKKRLMRDFKRLQQDPPAGISGAPQDN-NIMLWNAVIFGPDDTPWDGGTFKLTLQFTEDYPNKPPTVRFVSRMFHPNIYADGSICLDILQNXVE 379
            ++K L+ + K LQ++P  G      D  ++  W   IFGP +T ++GG FK  L+F  DYP  PP  RF+++M+HPNIY  G +C+ IL   V+
Sbjct:    9 SQKALLLELKGLQEEPVEGFRVTLVDEGDLYNWEVAIFGPPNTYYEGGYFKARLKFPIDYPYSPPAFRFLTKMWHPNIYETGDVCISILHPPVD 102

HSP 2 Score: 38.1206 bits (87), Expect = 3.166e-20
Identity = 24/56 (42.86%), Postives = 34/56 (60.71%), Query Frame = 3 
Query:  375 WSPIYDVAAILTSIQSLLCDPNPNSPANSEAA---RLFSENK---REYNRRVREIV 524
            W+P  +V  IL S+ SLL +PN  SPAN +A+   R + E+K   REY   +R+ V
Sbjct:  114 WNPTQNVRTILLSVISLLNEPNTFSPANVDASVMYRKWKESKGKDREYTDIIRKQV 169
[Close]
BLAST of EY675113 vs. ExPASy Swiss-Prot
Match: UBC9_CAEEL (SUMO-conjugating enzyme UBC9 OS=Caenorhabditis elegans GN=ubc-9 PE=1 SV=1)

HSP 1 Score: 75.8702 bits (185), Expect = 3.209e-20
Identity = 35/97 (36.08%), Postives = 53/97 (54.64%), Query Frame = 2 
Query:   89 MSTPAKKRLMRDFKRLQQDPPAGISGAPQDN-----NIMLWNAVIFGPDDTPWDGGTFKLTLQFTEDYPNKPPTVRFVSRMFHPNIYADGSICLDIL 364
            MS  A  RL  + K  ++D P G    P  N     N+  W   I G  DT W+GG +++ + F +D+P+ PP  +F   +FHPN+Y  G++CL +L
Sbjct:    1 MSGIAAGRLAEERKHWRKDHPFGFIAKPVKNADGTLNLFNWECAIPGRKDTIWEGGLYRIRMLFKDDFPSTPPKCKFEPPLFHPNVYPSGTVCLSLL 97

HSP 2 Score: 44.2838 bits (103), Expect = 3.209e-20
Identity = 18/48 (37.50%), Postives = 29/48 (60.42%), Query Frame = 3 
Query:  375 WSPIYDVAAILTSIQSLLCDPNPNSPANSEAARLFSENKREYNRRVRE 518
            W P   +  +L  IQ LL  PN   PA +EA +++ +N+ EY +RV++
Sbjct:  103 WKPSISIKQLLIGIQDLLNHPNIEDPAQAEAYQIYCQNRAEYEKRVKK 150
The following BLAST results are available for this feature:
BLAST of EY675113 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 228
Match NameE-valueIdentityDescription
UBC1_SCHPO8.522e-2134.74Ubiquitin-conjugating enzyme E2 1 OS=Schizosacchar... [more]
UBE2C_MACFA1.117e-2037.36Ubiquitin-conjugating enzyme E2 C OS=Macaca fascic... [more]
UBE2C_HUMAN1.453e-2037.78Ubiquitin-conjugating enzyme E2 C OS=Homo sapiens ... [more]
UBE2C_BOVIN1.890e-2037.36Ubiquitin-conjugating enzyme E2 C OS=Bos taurus GN... [more]
UBE2C_XENLA1.890e-2039.77Ubiquitin-conjugating enzyme E2 C OS=Xenopus laevi... [more]
UBC_ASFK52.440e-2038.10Ubiquitin-conjugating enzyme E2 OS=African swine f... [more]
UBC31_ARATH2.477e-2053.13Probable ubiquitin carrier protein E2 31 OS=Arabid... [more]
UB2R1_MOUSE3.166e-2040.43Ubiquitin-conjugating enzyme E2 R1 OS=Mus musculus... [more]
UB2R1_HUMAN3.166e-2040.43Ubiquitin-conjugating enzyme E2 R1 OS=Homo sapiens... [more]
UBC9_CAEEL3.209e-2036.08SUMO-conjugating enzyme UBC9 OS=Caenorhabditis ele... [more]

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Properties
Property NameValue
Genbank descriptionCS00-C1-401-006-C04-CT.F Sweet orange leaf, infected with CiLV Citrus sinensis cDNA, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>EY675113 ID=EY675113; Name=EY675113; organism=Citrus sinensis; type=EST; length=988bp
TTCTTTCTCTAGAAGCTTCTCCTCTCATTCTCCCCCCTTACCCCCTCTGC
AAAAGCCGCTTCTCAGTTTTGCTCTCTAAAACGCCACTATGTCAACACCG
GCGAAGAAGCGGCTGATGCGGGACTTCAAGCGCCTCCAGCAAGATCCACC
CGCCGGAATCAGCGGGGCCCCTCAAGACAACAACATCATGTTATGGAACG
CCGTTATTTTTGGGCCTGATGATACTCCCTGGGACGGAGGTACGTTCAAG
TTGACTCTTCAGTTTACAGAGGATTATCCGAATAAACCTCCAACAGTTCG
GTTCGTTTCACGGATGTTTCATCCAAATATCTATGCGGATGGAAGTATCT
GCCTGGATATATTACAAAATCANGTGGAGTCCTATATATGATGTTGCAGC
TATACTGACCTCCATCCAGTCGCTGCTTTGTGATCCAAATCCAAACTCCC
CTGCAAATTCTGAAGCAGCTCGGCTGTTTAGTGAGAATAAACGAGAGTAC
AACCGAAGAGTGCGTGAAATAGTGGAGCAAAGCTGGACAGCCGACTAAAA
GTCCCATGAAAGCACCGGCCGGTTATGCTGGAGAAATAAAAGATGAAAAC
TGGGGCTGGTGGGGTTGGTTTGGTCAAAATAATGGAACCCGTATTCAATC
GGATTTCAGGTGCATTGTTAATTAAAAAAATCCCCTTCGAATCTTTCCTC
CCCAAAAACCCTGGGTGGAACCCTTGGTTATTTTTCCAACATTTCCCTCT
TCCTTTTCCAAAACTTTCCGGGTAAATGTAATTTTATTGGGGACATTTTG
GTAAGGGGGGTTTTTTGGGGGGCGGGAAAACCCTGTGGATGTTGTTCTTT
AAAAAAACCCCCCCAAAAAAGAAACCCCTTTTTTTTTTTGGGAAATTTTT
TTTATGGGGGGATATTTTTTTGGGGGGATTTGTGGTGGGCGGGGGGGCGC
CCAAAAAAAAATAAAAAAAAAAAAAGGAAACAAAAACA
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