EY675113

Overview
NameEY675113
Unique NameEY675113
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length988
Libraries
Library NameType
Sweet orange leaf, infected with CiLVcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of EY675113 vs. ExPASy Swiss-Prot
Match: UBCD2_DROME (Ubiquitin-conjugating enzyme E2-24 kDa OS=Drosophila melanogaster GN=UbcD2 PE=1 SV=1)

HSP 1 Score: 81.2629 bits (199), Expect = 4.120e-20
Identity = 35/88 (39.77%), Postives = 55/88 (62.50%), Query Frame = 2 
Query:  107 KRLMRDFKRLQQDPPAGISGAPQDNNIMLWNAVIFGPDDTPWDGGTFKLTLQFTEDYPNKPPTVRFVSRMFHPNIYADGSICLDILQN 370
            KR+ ++   +  DPP   S  P+ +N+  W + I GP  + ++GG F L + F+ +YP KPP V F +R++H NI + G ICLDIL++
Sbjct:   89 KRIQKELAEITLDPPPNCSAGPKGDNLYEWVSTILGPPGSVYEGGVFFLDIHFSPEYPFKPPKVTFRTRIYHCNINSQGVICLDILKD 176

HSP 2 Score: 38.5058 bits (88), Expect = 4.120e-20
Identity = 19/50 (38.00%), Postives = 27/50 (54.00%), Query Frame = 3 
Query:  366 KIXWSPIYDVAAILTSIQSLLCDPNPNSPANSEAARLFSENKREYNRRVR 515
            K  WSP   ++ +L SI SLL D NP  P     A  + +N+ E++R  R
Sbjct:  175 KDNWSPALTISKVLLSICSLLTDCNPADPLVGSIATQYLQNREEHDRIAR 224
[Close]
BLAST of EY675113 vs. ExPASy Swiss-Prot
Match: UBE2C_MOUSE (Ubiquitin-conjugating enzyme E2 C OS=Mus musculus GN=Ube2c PE=2 SV=1)

HSP 1 Score: 73.9442 bits (180), Expect = 4.160e-20
Identity = 34/91 (37.36%), Postives = 52/91 (57.14%), Query Frame = 2 
Query:   98 PAKKRLMRDFKRLQQDPPAGISGAPQDNNIMLWNAVIFGPDDTPWDGGTFKLTLQFTEDYPNKPPTVRFVSRMFHPNIYADGSICLDILQN 370
            P  KRL ++   L      GIS  P+ +N+  W   I G   T ++   +KL+L+F   YP   PTV+F++  +HPN+   G+ICLDIL++
Sbjct:   30 PVGKRLQQELMILMTSGDKGISAFPESDNLFKWVGTIHGAAGTVYEDLRYKLSLEFPSGYPYNAPTVKFLTPCYHPNVDTQGNICLDILKD 120

HSP 2 Score: 45.8246 bits (107), Expect = 4.160e-20
Identity = 24/51 (47.06%), Postives = 34/51 (66.67%), Query Frame = 3 
Query:  366 KIXWSPIYDVAAILTSIQSLLCDPNPNSPANSEAARLFSENKREYNRRVRE 518
            K  WS +YDV  IL SIQSLL +PN +SP N+ AA L+ +N   + + ++E
Sbjct:  119 KDKWSALYDVRTILLSIQSLLGEPNIDSPLNTHAAELW-KNPTAFKKYLQE 168
[Close]
BLAST of EY675113 vs. ExPASy Swiss-Prot
Match: UBC21_ARATH (Probable ubiquitin carrier protein E2 21 OS=Arabidopsis thaliana GN=UBC21 PE=1 SV=1)

HSP 1 Score: 85.5001 bits (210), Expect = 2.028e-19
Identity = 40/93 (43.01%), Postives = 61/93 (65.59%), Query Frame = 2 
Query:  101 AKKRLMRDFKRLQQDPPAG--ISGAPQDNNIMLWNAVIFGPDDTPWDGGTFKLTLQFTEDYPNKPPTVRFVSRMFHPNI-YADGSICLDILQN 370
            ++ RL +++K +Q++  A   I     D NI  W A+I GP +TP++GG F+L     E YP +PP VRF++++FHPN+ +  G ICLDIL+N
Sbjct:    4 SRARLFKEYKEVQREKVADPDIQLICDDTNIFKWTALIKGPSETPYEGGVFQLAFSVPEPYPLQPPQVRFLTKIFHPNVHFKTGEICLDILKN 96

HSP 2 Score: 31.9574 bits (71), Expect = 2.028e-19
Identity = 15/47 (31.91%), Postives = 27/47 (57.45%), Query Frame = 3 
Query:  366 KIXWSPIYDVAAILTSIQSLLCDPNPNSPANSEAARLF-SENKREYN 503
            K  WSP + + ++  +I +L+  P P+SP N ++  L  S + R +N
Sbjct:   95 KNAWSPAWTLQSVCRAIIALMAHPEPDSPLNCDSGNLLRSGDVRGFN 141
[Close]
BLAST of EY675113 vs. ExPASy Swiss-Prot
Match: COP10_ARATH (Constitutive photomorphogenesis protein 10 OS=Arabidopsis thaliana GN=COP10 PE=1 SV=1)

HSP 1 Score: 78.9518 bits (193), Expect = 1.267e-18
Identity = 36/95 (37.89%), Postives = 56/95 (58.95%), Query Frame = 2 
Query:   86 TMSTPAKKRLMRDFKRLQQDPPAGISGAPQDNNIMLWNAVIFGPDDTPWDGGTFKLTLQFTEDYPNKPPTVRFVSRMFHPNIYADGSICLDILQN 370
            T  + + KR+ R+   L  DPP   S  P+ +N+  W A I GP  TP++GG F L + F  DYP KPP + F +R++H N+   G + ++IL++
Sbjct:   32 TSVSASGKRIQREMAELNIDPPPDCSAGPKGDNLYHWIATIIGPSGTPYEGGIFFLDIIFPSDYPFKPPKLVFKTRIYHCNVDTAGDLSVNILRD 126

HSP 2 Score: 35.8094 bits (81), Expect = 1.267e-18
Identity = 15/48 (31.25%), Postives = 27/48 (56.25%), Query Frame = 3 
Query:  375 WSPIYDVAAILTSIQSLLCDPNPNSPANSEAARLFSENKREYNRRVRE 518
            WSP   +  +L +I+S+   P P SPA    ARL+  ++ +++   +E
Sbjct:  128 WSPALTITKVLQAIRSIFLKPEPYSPALPVIARLYLTDREKHDEVAKE 175
[Close]
BLAST of EY675113 vs. ExPASy Swiss-Prot
Match: UBE2S_HUMAN (Ubiquitin-conjugating enzyme E2 S OS=Homo sapiens GN=UBE2S PE=1 SV=2)

HSP 1 Score: 71.2478 bits (173), Expect = 1.634e-18
Identity = 30/83 (36.14%), Postives = 51/83 (61.45%), Query Frame = 2 
Query:  119 RDFKRLQQDPPAGISGAPQDNNIMLWNAVIFGPDDTPWDGGTFKLTLQFTEDYPNKPPTVRFVSRMFHPNIYADGSICLDILQ 367
            ++   L  DPP GI   P + ++      I GP+ TP+ GG F++ L   +D+P  PP   F++++FHPN+ A+G IC+++L+
Sbjct:   18 KEVTTLTADPPDGIKVFPNEEDLTDLQVTIEGPEGTPYAGGLFRMKLLLGKDFPASPPKGYFLTKIFHPNVGANGEICVNVLK 100

HSP 2 Score: 43.1282 bits (100), Expect = 1.634e-18
Identity = 22/54 (40.74%), Postives = 28/54 (51.85%), Query Frame = 3 
Query:  366 KIXWSPIYDVAAILTSIQSLLCDPNPNSPANSEAARLFSENKREYNRRVREIVE 527
            K  W+    +  +L +I+ LL  PNP S  N EA RL  EN  EY  R R + E
Sbjct:  100 KRDWTAELGIRHVLLTIKCLLIHPNPESALNEEAGRLLLENYEEYAARARLLTE 153
[Close]
BLAST of EY675113 vs. ExPASy Swiss-Prot
Match: UBE2S_BOVIN (Ubiquitin-conjugating enzyme E2 S OS=Bos taurus GN=UBE2S PE=2 SV=1)

HSP 1 Score: 71.2478 bits (173), Expect = 1.634e-18
Identity = 30/83 (36.14%), Postives = 51/83 (61.45%), Query Frame = 2 
Query:  119 RDFKRLQQDPPAGISGAPQDNNIMLWNAVIFGPDDTPWDGGTFKLTLQFTEDYPNKPPTVRFVSRMFHPNIYADGSICLDILQ 367
            ++   L  DPP GI   P + ++      I GP+ TP+ GG F++ L   +D+P  PP   F++++FHPN+ A+G IC+++L+
Sbjct:   18 KEVTTLTADPPDGIKVFPNEEDLTDLQVTIEGPEGTPYAGGLFRMKLLLGKDFPASPPKGYFLTKIFHPNVGANGEICVNVLK 100

HSP 2 Score: 43.1282 bits (100), Expect = 1.634e-18
Identity = 22/54 (40.74%), Postives = 28/54 (51.85%), Query Frame = 3 
Query:  366 KIXWSPIYDVAAILTSIQSLLCDPNPNSPANSEAARLFSENKREYNRRVREIVE 527
            K  W+    +  +L +I+ LL  PNP S  N EA RL  EN  EY  R R + E
Sbjct:  100 KRDWTAELGIRHVLLTIKCLLIHPNPESALNEEAGRLLLENYEEYAARARLLTE 153
[Close]
BLAST of EY675113 vs. ExPASy Swiss-Prot
Match: UBE2S_DROAN (Ubiquitin-conjugating enzyme E2 S OS=Drosophila ananassae GN=GF21161 PE=3 SV=1)

HSP 1 Score: 72.0182 bits (175), Expect = 2.344e-18
Identity = 30/93 (32.26%), Postives = 57/93 (61.29%), Query Frame = 2 
Query:   89 MSTPAKKRLMRDFKRLQQDPPAGISGAPQDNNIMLWNAVIFGPDDTPWDGGTFKLTLQFTEDYPNKPPTVRFVSRMFHPNIYADGSICLDILQ 367
            +S    +++MR+ + ++  PP GI     ++++    A+I GP  TP+  G F++ L   +D+P  PP   F++++FHPN+ A+G IC++ L+
Sbjct:   11 LSPQTIRQVMRELQEMETTPPEGIKVLINESDVTDIQALIDGPAGTPYAAGIFRVKLTLNKDFPQTPPKAYFLTKIFHPNVAANGEICVNTLK 103

HSP 2 Score: 41.2022 bits (95), Expect = 2.344e-18
Identity = 20/54 (37.04%), Postives = 29/54 (53.70%), Query Frame = 3 
Query:  366 KIXWSPIYDVAAILTSIQSLLCDPNPNSPANSEAARLFSENKREYNRRVREIVE 527
            K  W P   +  IL +I+ LL  PNP S  N EA ++  E   +Y++R R + E
Sbjct:  103 KKDWKPDLGIKHILLTIKCLLIVPNPESALNEEAGKMLLERYDDYSQRARMMTE 156

HSP 3 Score: 20.4014 bits (41), Expect = 2.344e-18
Identity = 8/15 (53.33%), Postives = 10/15 (66.67%), Query Frame = 1 
Query:  553 PMKAPAGYAGEIKDE 597
            P K  AG AG+ KD+
Sbjct:  161 PAKCAAGAAGDSKDD 175
[Close]
BLAST of EY675113 vs. ExPASy Swiss-Prot
Match: UBE2S_RANCA (Ubiquitin-conjugating enzyme E2 S OS=Rana catesbeiana GN=ube2s PE=2 SV=1)

HSP 1 Score: 71.2478 bits (173), Expect = 2.769e-18
Identity = 30/87 (34.48%), Postives = 54/87 (62.07%), Query Frame = 2 
Query:  107 KRLMRDFKRLQQDPPAGISGAPQDNNIMLWNAVIFGPDDTPWDGGTFKLTLQFTEDYPNKPPTVRFVSRMFHPNIYADGSICLDILQ 367
            +++ ++   L  DPP GI   P + +I      I GP+ TP+ GG F++ L   +D+P  PP   F++++FHPN+ ++G IC+++L+
Sbjct:   14 RQVYKEVSTLTPDPPEGIKIIPNEEDITDVQVSIEGPEGTPYAGGIFRMKLILGKDFPAAPPKGYFLTKIFHPNVSSNGEICVNVLK 100

HSP 2 Score: 42.3578 bits (98), Expect = 2.769e-18
Identity = 22/54 (40.74%), Postives = 27/54 (50.00%), Query Frame = 3 
Query:  366 KIXWSPIYDVAAILTSIQSLLCDPNPNSPANSEAARLFSENKREYNRRVREIVE 527
            K  W     +  +L +I+ LL  PNP S  N EA RL  EN  EY  R R + E
Sbjct:  100 KKDWKAELGIRHVLLTIKCLLIHPNPESALNEEAGRLLLENYEEYASRARLMTE 153
[Close]
BLAST of EY675113 vs. ExPASy Swiss-Prot
Match: UBE2S_DROYA (Ubiquitin-conjugating enzyme E2 S OS=Drosophila yakuba GN=GE15576 PE=3 SV=1)

HSP 1 Score: 72.4034 bits (176), Expect = 2.771e-18
Identity = 30/93 (32.26%), Postives = 57/93 (61.29%), Query Frame = 2 
Query:   89 MSTPAKKRLMRDFKRLQQDPPAGISGAPQDNNIMLWNAVIFGPDDTPWDGGTFKLTLQFTEDYPNKPPTVRFVSRMFHPNIYADGSICLDILQ 367
            +S    +++MR+ + ++  PP GI     ++++    A+I GP  TP+  G F++ L   +D+P  PP   F++++FHPN+ A+G IC++ L+
Sbjct:   11 LSPQTIRQVMRELQEMETTPPEGIKVLINESDVTDIQALIDGPAGTPYAAGVFRVKLTLNKDFPQTPPKAYFLTKIFHPNVAANGEICVNTLK 103

HSP 2 Score: 41.2022 bits (95), Expect = 2.771e-18
Identity = 20/54 (37.04%), Postives = 29/54 (53.70%), Query Frame = 3 
Query:  366 KIXWSPIYDVAAILTSIQSLLCDPNPNSPANSEAARLFSENKREYNRRVREIVE 527
            K  W P   +  IL +I+ LL  PNP S  N EA ++  E   +Y++R R + E
Sbjct:  103 KKDWKPDLGIKHILLTIKCLLIVPNPESALNEEAGKMLLERYDDYSQRARMMTE 156
[Close]
BLAST of EY675113 vs. ExPASy Swiss-Prot
Match: UBE2S_DROER (Ubiquitin-conjugating enzyme E2 S OS=Drosophila erecta GN=GG18165 PE=3 SV=1)

HSP 1 Score: 72.4034 bits (176), Expect = 2.771e-18
Identity = 30/93 (32.26%), Postives = 57/93 (61.29%), Query Frame = 2 
Query:   89 MSTPAKKRLMRDFKRLQQDPPAGISGAPQDNNIMLWNAVIFGPDDTPWDGGTFKLTLQFTEDYPNKPPTVRFVSRMFHPNIYADGSICLDILQ 367
            +S    +++MR+ + ++  PP GI     ++++    A+I GP  TP+  G F++ L   +D+P  PP   F++++FHPN+ A+G IC++ L+
Sbjct:   11 LSPQTIRQVMRELQEMETTPPEGIKVLINESDVTDIQALIDGPAGTPYAAGVFRVKLTLNKDFPQTPPKAYFLTKIFHPNVAANGEICVNTLK 103

HSP 2 Score: 41.2022 bits (95), Expect = 2.771e-18
Identity = 20/54 (37.04%), Postives = 29/54 (53.70%), Query Frame = 3 
Query:  366 KIXWSPIYDVAAILTSIQSLLCDPNPNSPANSEAARLFSENKREYNRRVREIVE 527
            K  W P   +  IL +I+ LL  PNP S  N EA ++  E   +Y++R R + E
Sbjct:  103 KKDWKPDLGIKHILLTIKCLLIVPNPESALNEEAGKMLLERYDDYSQRARMMTE 156
The following BLAST results are available for this feature:
BLAST of EY675113 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 228
Match NameE-valueIdentityDescription
UBCD2_DROME4.120e-2039.77Ubiquitin-conjugating enzyme E2-24 kDa OS=Drosophi... [more]
UBE2C_MOUSE4.160e-2037.36Ubiquitin-conjugating enzyme E2 C OS=Mus musculus ... [more]
UBC21_ARATH2.028e-1943.01Probable ubiquitin carrier protein E2 21 OS=Arabid... [more]
COP10_ARATH1.267e-1837.89Constitutive photomorphogenesis protein 10 OS=Arab... [more]
UBE2S_HUMAN1.634e-1836.14Ubiquitin-conjugating enzyme E2 S OS=Homo sapiens ... [more]
UBE2S_BOVIN1.634e-1836.14Ubiquitin-conjugating enzyme E2 S OS=Bos taurus GN... [more]
UBE2S_DROAN2.344e-1832.26Ubiquitin-conjugating enzyme E2 S OS=Drosophila an... [more]
UBE2S_RANCA2.769e-1834.48Ubiquitin-conjugating enzyme E2 S OS=Rana catesbei... [more]
UBE2S_DROYA2.771e-1832.26Ubiquitin-conjugating enzyme E2 S OS=Drosophila ya... [more]
UBE2S_DROER2.771e-1832.26Ubiquitin-conjugating enzyme E2 S OS=Drosophila er... [more]

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Properties
Property NameValue
Genbank descriptionCS00-C1-401-006-C04-CT.F Sweet orange leaf, infected with CiLV Citrus sinensis cDNA, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>EY675113 ID=EY675113; Name=EY675113; organism=Citrus sinensis; type=EST; length=988bp
TTCTTTCTCTAGAAGCTTCTCCTCTCATTCTCCCCCCTTACCCCCTCTGC
AAAAGCCGCTTCTCAGTTTTGCTCTCTAAAACGCCACTATGTCAACACCG
GCGAAGAAGCGGCTGATGCGGGACTTCAAGCGCCTCCAGCAAGATCCACC
CGCCGGAATCAGCGGGGCCCCTCAAGACAACAACATCATGTTATGGAACG
CCGTTATTTTTGGGCCTGATGATACTCCCTGGGACGGAGGTACGTTCAAG
TTGACTCTTCAGTTTACAGAGGATTATCCGAATAAACCTCCAACAGTTCG
GTTCGTTTCACGGATGTTTCATCCAAATATCTATGCGGATGGAAGTATCT
GCCTGGATATATTACAAAATCANGTGGAGTCCTATATATGATGTTGCAGC
TATACTGACCTCCATCCAGTCGCTGCTTTGTGATCCAAATCCAAACTCCC
CTGCAAATTCTGAAGCAGCTCGGCTGTTTAGTGAGAATAAACGAGAGTAC
AACCGAAGAGTGCGTGAAATAGTGGAGCAAAGCTGGACAGCCGACTAAAA
GTCCCATGAAAGCACCGGCCGGTTATGCTGGAGAAATAAAAGATGAAAAC
TGGGGCTGGTGGGGTTGGTTTGGTCAAAATAATGGAACCCGTATTCAATC
GGATTTCAGGTGCATTGTTAATTAAAAAAATCCCCTTCGAATCTTTCCTC
CCCAAAAACCCTGGGTGGAACCCTTGGTTATTTTTCCAACATTTCCCTCT
TCCTTTTCCAAAACTTTCCGGGTAAATGTAATTTTATTGGGGACATTTTG
GTAAGGGGGGTTTTTTGGGGGGCGGGAAAACCCTGTGGATGTTGTTCTTT
AAAAAAACCCCCCCAAAAAAGAAACCCCTTTTTTTTTTTGGGAAATTTTT
TTTATGGGGGGATATTTTTTTGGGGGGATTTGTGGTGGGCGGGGGGGCGC
CCAAAAAAAAATAAAAAAAAAAAAAGGAAACAAAAACA
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