EY675113

Overview
NameEY675113
Unique NameEY675113
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length988
Libraries
Library NameType
Sweet orange leaf, infected with CiLVcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of EY675113 vs. ExPASy Swiss-Prot
Match: UBE2T_XENLA (Ubiquitin-conjugating enzyme E2 T OS=Xenopus laevis GN=ube2t PE=2 SV=1)

HSP 1 Score: 72.7886 bits (177), Expect = 6.110e-18
Identity = 34/86 (39.53%), Postives = 54/86 (62.79%), Query Frame = 2 
Query:  110 RLMRDFKRLQQDPPAGISGAPQDNNIMLWNAVIFGPDDTPWDGGTFKLTLQFTEDYPNKPPTVRFVSRMFHPNIYADGSICLDILQ 367
            RL R+ + L ++PP G+     ++N+    A I G   +P++GG F L +   E YP +PP +RF++ ++HPNI + G ICLDIL+
Sbjct:    6 RLKRELQLLNKEPPPGVICWQNESNMDDLRAQIIGGSGSPYEGGIFNLEIIVPERYPFEPPKIRFLTPIYHPNIDSAGRICLDILK 91

HSP 2 Score: 39.6614 bits (91), Expect = 6.110e-18
Identity = 19/55 (34.55%), Postives = 31/55 (56.36%), Query Frame = 3 
Query:  375 WSPIYDVAAILTSIQSLLCDPNPNSPANSEAARLFSENKREYNRRVREIVEQSWT 539
            W P  +++ +LTSIQ L+ +PNP+ P  ++ +  F     +YNR V     + WT
Sbjct:   98 WRPALNISTVLTSIQLLMSEPNPDDPLMADISSEF-----KYNRAVFFSNAKKWT 147
[Close]
BLAST of EY675113 vs. ExPASy Swiss-Prot
Match: UBE2S_DROSI (Ubiquitin-conjugating enzyme E2 S OS=Drosophila simulans GN=GD17343 PE=3 SV=1)

HSP 1 Score: 70.8626 bits (172), Expect = 7.921e-18
Identity = 30/93 (32.26%), Postives = 57/93 (61.29%), Query Frame = 2 
Query:   89 MSTPAKKRLMRDFKRLQQDPPAGISGAPQDNNIMLWNAVIFGPDDTPWDGGTFKLTLQFTEDYPNKPPTVRFVSRMFHPNIYADGSICLDILQ 367
            +S    +++MR+ + ++  PP GI     ++++    A+I GP  TP+  G F++ L   +D+P  PP   F++++FHPN+ A+G IC++ L+
Sbjct:   11 LSPQTIRQVMRELQEMETTPPEGIKVLINESDVTDIQALIDGPAGTPYAAGIFRVKLTLNKDFPLTPPKAYFLTKIFHPNVAANGEICVNTLK 103

HSP 2 Score: 41.2022 bits (95), Expect = 7.921e-18
Identity = 20/54 (37.04%), Postives = 29/54 (53.70%), Query Frame = 3 
Query:  366 KIXWSPIYDVAAILTSIQSLLCDPNPNSPANSEAARLFSENKREYNRRVREIVE 527
            K  W P   +  IL +I+ LL  PNP S  N EA ++  E   +Y++R R + E
Sbjct:  103 KKDWKPDLGIKHILLTIKCLLIVPNPESALNEEAGKMLLERYDDYSQRARMMTE 156
[Close]
BLAST of EY675113 vs. ExPASy Swiss-Prot
Match: UBE2S_DROSE (Ubiquitin-conjugating enzyme E2 S OS=Drosophila sechellia GN=GM13299 PE=3 SV=1)

HSP 1 Score: 70.8626 bits (172), Expect = 7.921e-18
Identity = 30/93 (32.26%), Postives = 57/93 (61.29%), Query Frame = 2 
Query:   89 MSTPAKKRLMRDFKRLQQDPPAGISGAPQDNNIMLWNAVIFGPDDTPWDGGTFKLTLQFTEDYPNKPPTVRFVSRMFHPNIYADGSICLDILQ 367
            +S    +++MR+ + ++  PP GI     ++++    A+I GP  TP+  G F++ L   +D+P  PP   F++++FHPN+ A+G IC++ L+
Sbjct:   11 LSPQTIRQVMRELQEMETTPPEGIKVLINESDVTDIQALIDGPAGTPYAAGIFRVKLTLNKDFPLTPPKAYFLTKIFHPNVAANGEICVNTLK 103

HSP 2 Score: 41.2022 bits (95), Expect = 7.921e-18
Identity = 20/54 (37.04%), Postives = 29/54 (53.70%), Query Frame = 3 
Query:  366 KIXWSPIYDVAAILTSIQSLLCDPNPNSPANSEAARLFSENKREYNRRVREIVE 527
            K  W P   +  IL +I+ LL  PNP S  N EA ++  E   +Y++R R + E
Sbjct:  103 KKDWKPDLGIKHILLTIKCLLIVPNPESALNEEAGKMLLERYDDYSQRARMMTE 156
[Close]
BLAST of EY675113 vs. ExPASy Swiss-Prot
Match: UBE2S_DROME (Ubiquitin-conjugating enzyme E2 S OS=Drosophila melanogaster GN=CG8188 PE=1 SV=2)

HSP 1 Score: 70.8626 bits (172), Expect = 7.921e-18
Identity = 30/93 (32.26%), Postives = 57/93 (61.29%), Query Frame = 2 
Query:   89 MSTPAKKRLMRDFKRLQQDPPAGISGAPQDNNIMLWNAVIFGPDDTPWDGGTFKLTLQFTEDYPNKPPTVRFVSRMFHPNIYADGSICLDILQ 367
            +S    +++MR+ + ++  PP GI     ++++    A+I GP  TP+  G F++ L   +D+P  PP   F++++FHPN+ A+G IC++ L+
Sbjct:   11 LSPQTIRQVMRELQEMETTPPEGIKVLINESDVTDIQALIDGPAGTPYAAGIFRVKLTLNKDFPLTPPKAYFLTKIFHPNVAANGEICVNTLK 103

HSP 2 Score: 41.2022 bits (95), Expect = 7.921e-18
Identity = 20/54 (37.04%), Postives = 29/54 (53.70%), Query Frame = 3 
Query:  366 KIXWSPIYDVAAILTSIQSLLCDPNPNSPANSEAARLFSENKREYNRRVREIVE 527
            K  W P   +  IL +I+ LL  PNP S  N EA ++  E   +Y++R R + E
Sbjct:  103 KKDWKPDLGIKHILLTIKCLLIVPNPESALNEEAGKMLLERYDDYSQRARMMTE 156
[Close]
BLAST of EY675113 vs. ExPASy Swiss-Prot
Match: UBC14_SCHPO (Ubiquitin-conjugating enzyme E2 14 OS=Schizosaccharomyces pombe GN=ubc14 PE=2 SV=1)

HSP 1 Score: 79.7221 bits (195), Expect = 8.041e-18
Identity = 35/98 (35.71%), Postives = 59/98 (60.20%), Query Frame = 2 
Query:   83 ATMSTPAKKRLMRDFKRLQQDPPAGISGAPQDNNIMLWNAVIFGPDDTPWDGGTFKLTLQFTEDYPNKPPTVRFVSRMFHPNIYADGSICLDILQNXV 376
            A+ S  + +RL +++  L++ P   I     D+N+  W     GP D+ + GG F  +L+F  DYP +PPT+ F +R++HPN  ++G++CL IL+  V
Sbjct:    2 ASASPSSSRRLTKEYSDLREHPIPDIRVNLVDDNLFHWACTALGPSDSVYAGGKFHFSLKFPLDYPFQPPTIEFTTRIYHPNFDSEGNVCLAILKQQV 99

HSP 2 Score: 32.3426 bits (72), Expect = 8.041e-18
Identity = 14/52 (26.92%), Postives = 28/52 (53.85%), Query Frame = 3 
Query:  375 WSPIYDVAAILTSIQSLLCDPNPNSPANSEAARLFSENKREYNRRVREIVEQ 530
            + P   + ++L  I  LL +PNP+ P  +  A  +  ++  +++  R+ VEQ
Sbjct:  100 FKPSIKLRSVLEQILQLLREPNPDDPLVASIAEQYRNDRPSFDKIARDYVEQ 151
[Close]
BLAST of EY675113 vs. ExPASy Swiss-Prot
Match: UBCX_DICDI (Ubiquitin-conjugating enzyme E2 pex4 OS=Dictyostelium discoideum GN=pex4 PE=3 SV=1)

HSP 1 Score: 80.4925 bits (197), Expect = 8.065e-18
Identity = 38/88 (43.18%), Postives = 61/88 (69.32%), Query Frame = 2 
Query:  110 RLMRDFKRLQQDPPAGISGAPQD-NNIMLWNAVIFGPDDTPWDGGTFKLTLQFTEDYPNKPPTVRFVSRMFHPNI-YADGSICLDILQ 367
            RLM+++K LQ +    I   P+D +++  W A+I GP DTP++ G F+L +    +YP +PPT++FV+++FHPNI +  G ICLD+L+
Sbjct:    4 RLMKEYKVLQNEEFEDILLYPRDESDLYRWVAIIKGPPDTPYENGKFELDISVPTNYPLQPPTIKFVTKIFHPNIHFKTGEICLDLLK 91

HSP 2 Score: 31.5722 bits (70), Expect = 8.065e-18
Identity = 15/37 (40.54%), Postives = 21/37 (56.76%), Query Frame = 3 
Query:  366 KIXWSPIYDVAAILTSIQSLLCDPNPNSPANSEAARL 476
            K  WS IY + ++  SI +LL  P  +SP N +A  L
Sbjct:   91 KTSWSAIYTLQSVCRSIIALLSLPEADSPLNCDAGNL 127
[Close]
BLAST of EY675113 vs. ExPASy Swiss-Prot
Match: UBE2T_MOUSE (Ubiquitin-conjugating enzyme E2 T OS=Mus musculus GN=Ube2t PE=2 SV=1)

HSP 1 Score: 69.3218 bits (168), Expect = 1.031e-17
Identity = 34/86 (39.53%), Postives = 52/86 (60.47%), Query Frame = 2 
Query:  110 RLMRDFKRLQQDPPAGISGAPQDNNIMLWNAVIFGPDDTPWDGGTFKLTLQFTEDYPNKPPTVRFVSRMFHPNIYADGSICLDILQ 367
            RL ++   L  +PP GI+   + + +    A I G  +TP++ G F L +   E YP +PP VRF++ ++HPNI + G ICLDIL+
Sbjct:    6 RLKKELHMLAIEPPPGITCWQEKDQVADLRAQILGGANTPYEKGVFTLEVIIPERYPFEPPQVRFLTPIYHPNIDSSGRICLDILK 91

HSP 2 Score: 42.3578 bits (98), Expect = 1.031e-17
Identity = 17/51 (33.33%), Postives = 31/51 (60.78%), Query Frame = 3 
Query:  375 WSPIYDVAAILTSIQSLLCDPNPNSPANSEAARLFSENKREYNRRVREIVE 527
            W P  ++A +LTSIQ L+ +PNP+ P  ++ +  F  NK  + ++ ++  E
Sbjct:   98 WRPSLNIATVLTSIQLLMAEPNPDDPLMADISSEFKYNKIAFLKKAKQWTE 148
[Close]
BLAST of EY675113 vs. ExPASy Swiss-Prot
Match: UBE2S_DROPS (Ubiquitin-conjugating enzyme E2 S OS=Drosophila pseudoobscura pseudoobscura GN=GA22810 PE=3 SV=1)

HSP 1 Score: 69.707 bits (169), Expect = 1.741e-17
Identity = 29/93 (31.18%), Postives = 57/93 (61.29%), Query Frame = 2 
Query:   89 MSTPAKKRLMRDFKRLQQDPPAGISGAPQDNNIMLWNAVIFGPDDTPWDGGTFKLTLQFTEDYPNKPPTVRFVSRMFHPNIYADGSICLDILQ 367
            +S    +++MR+ + ++  PP GI     ++++    A+I GP  TP+  G F++ L  ++D+P  PP   F++++FHPN+  +G IC++ L+
Sbjct:   11 LSPQTIRQVMRELQEMETTPPEGIKVLINESDVTDIQALIDGPAGTPYAVGIFRVKLTLSKDFPQTPPKAYFLTKIFHPNVAGNGEICVNTLK 103

HSP 2 Score: 41.2022 bits (95), Expect = 1.741e-17
Identity = 20/54 (37.04%), Postives = 29/54 (53.70%), Query Frame = 3 
Query:  366 KIXWSPIYDVAAILTSIQSLLCDPNPNSPANSEAARLFSENKREYNRRVREIVE 527
            K  W P   +  IL +I+ LL  PNP S  N EA ++  E   +Y++R R + E
Sbjct:  103 KKDWKPDLGIKHILLTIKCLLIVPNPESALNEEAGKMLLERYDDYSQRARMMTE 156
[Close]
BLAST of EY675113 vs. ExPASy Swiss-Prot
Match: UBE2S_DROPE (Ubiquitin-conjugating enzyme E2 S OS=Drosophila persimilis GN=GL16001 PE=3 SV=1)

HSP 1 Score: 69.707 bits (169), Expect = 1.741e-17
Identity = 29/93 (31.18%), Postives = 57/93 (61.29%), Query Frame = 2 
Query:   89 MSTPAKKRLMRDFKRLQQDPPAGISGAPQDNNIMLWNAVIFGPDDTPWDGGTFKLTLQFTEDYPNKPPTVRFVSRMFHPNIYADGSICLDILQ 367
            +S    +++MR+ + ++  PP GI     ++++    A+I GP  TP+  G F++ L  ++D+P  PP   F++++FHPN+  +G IC++ L+
Sbjct:   11 LSPQTIRQVMRELQEMETTPPEGIKVLINESDVTDIQALIDGPAGTPYAVGIFRVKLTLSKDFPQTPPKAYFLTKIFHPNVAGNGEICVNTLK 103

HSP 2 Score: 41.2022 bits (95), Expect = 1.741e-17
Identity = 20/54 (37.04%), Postives = 29/54 (53.70%), Query Frame = 3 
Query:  366 KIXWSPIYDVAAILTSIQSLLCDPNPNSPANSEAARLFSENKREYNRRVREIVE 527
            K  W P   +  IL +I+ LL  PNP S  N EA ++  E   +Y++R R + E
Sbjct:  103 KKDWKPDLGIKHILLTIKCLLIVPNPESALNEEAGKMLLERYDDYSQRARMMTE 156
[Close]
BLAST of EY675113 vs. ExPASy Swiss-Prot
Match: UBC37_ARATH (Probable ubiquitin carrier protein E2 37 OS=Arabidopsis thaliana GN=UBC37 PE=2 SV=1)

HSP 1 Score: 68.1662 bits (165), Expect = 2.219e-17
Identity = 37/94 (39.36%), Postives = 50/94 (53.19%), Query Frame = 2 
Query:  110 RLMRDFKRLQQDPPAGIS---------GAPQDNNIMLWNAVIFGPDDTPWDGGTFKLTLQFTEDYPNKPPTVRFVSRMFHPNIYADGSICLDIL 364
            R+ ++ K L  DPP G S         G+   +     +A I GP+DT +  G F L +Q  E YP +PP V F + ++HPNI   G ICLDIL
Sbjct:   10 RMQKELKLLLSDPPHGASFPHLSSAANGSGDFSTFSTIDAQIEGPEDTVYANGIFNLKIQIPERYPFQPPIVSFATPIYHPNIDNSGRICLDIL 103

HSP 2 Score: 42.3578 bits (98), Expect = 2.219e-17
Identity = 16/52 (30.77%), Postives = 33/52 (63.46%), Query Frame = 3 
Query:  375 WSPIYDVAAILTSIQSLLCDPNPNSPANSEAARLFSENKREYNRRVREIVEQ 530
            W P  +++ +LTS++ LL +PNP+     E +R +  N++ ++ + RE+ E+
Sbjct:  111 WQPSLNISTVLTSMRLLLSEPNPDDGLMCEVSREYKYNRQTFDYKAREMTEK 162
The following BLAST results are available for this feature:
BLAST of EY675113 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 228
Match NameE-valueIdentityDescription
UBE2T_XENLA6.110e-1839.53Ubiquitin-conjugating enzyme E2 T OS=Xenopus laevi... [more]
UBE2S_DROSI7.921e-1832.26Ubiquitin-conjugating enzyme E2 S OS=Drosophila si... [more]
UBE2S_DROSE7.921e-1832.26Ubiquitin-conjugating enzyme E2 S OS=Drosophila se... [more]
UBE2S_DROME7.921e-1832.26Ubiquitin-conjugating enzyme E2 S OS=Drosophila me... [more]
UBC14_SCHPO8.041e-1835.71Ubiquitin-conjugating enzyme E2 14 OS=Schizosaccha... [more]
UBCX_DICDI8.065e-1843.18Ubiquitin-conjugating enzyme E2 pex4 OS=Dictyostel... [more]
UBE2T_MOUSE1.031e-1739.53Ubiquitin-conjugating enzyme E2 T OS=Mus musculus ... [more]
UBE2S_DROPS1.741e-1731.18Ubiquitin-conjugating enzyme E2 S OS=Drosophila ps... [more]
UBE2S_DROPE1.741e-1731.18Ubiquitin-conjugating enzyme E2 S OS=Drosophila pe... [more]
UBC37_ARATH2.219e-1739.36Probable ubiquitin carrier protein E2 37 OS=Arabid... [more]

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Properties
Property NameValue
Genbank descriptionCS00-C1-401-006-C04-CT.F Sweet orange leaf, infected with CiLV Citrus sinensis cDNA, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>EY675113 ID=EY675113; Name=EY675113; organism=Citrus sinensis; type=EST; length=988bp
TTCTTTCTCTAGAAGCTTCTCCTCTCATTCTCCCCCCTTACCCCCTCTGC
AAAAGCCGCTTCTCAGTTTTGCTCTCTAAAACGCCACTATGTCAACACCG
GCGAAGAAGCGGCTGATGCGGGACTTCAAGCGCCTCCAGCAAGATCCACC
CGCCGGAATCAGCGGGGCCCCTCAAGACAACAACATCATGTTATGGAACG
CCGTTATTTTTGGGCCTGATGATACTCCCTGGGACGGAGGTACGTTCAAG
TTGACTCTTCAGTTTACAGAGGATTATCCGAATAAACCTCCAACAGTTCG
GTTCGTTTCACGGATGTTTCATCCAAATATCTATGCGGATGGAAGTATCT
GCCTGGATATATTACAAAATCANGTGGAGTCCTATATATGATGTTGCAGC
TATACTGACCTCCATCCAGTCGCTGCTTTGTGATCCAAATCCAAACTCCC
CTGCAAATTCTGAAGCAGCTCGGCTGTTTAGTGAGAATAAACGAGAGTAC
AACCGAAGAGTGCGTGAAATAGTGGAGCAAAGCTGGACAGCCGACTAAAA
GTCCCATGAAAGCACCGGCCGGTTATGCTGGAGAAATAAAAGATGAAAAC
TGGGGCTGGTGGGGTTGGTTTGGTCAAAATAATGGAACCCGTATTCAATC
GGATTTCAGGTGCATTGTTAATTAAAAAAATCCCCTTCGAATCTTTCCTC
CCCAAAAACCCTGGGTGGAACCCTTGGTTATTTTTCCAACATTTCCCTCT
TCCTTTTCCAAAACTTTCCGGGTAAATGTAATTTTATTGGGGACATTTTG
GTAAGGGGGGTTTTTTGGGGGGCGGGAAAACCCTGTGGATGTTGTTCTTT
AAAAAAACCCCCCCAAAAAAGAAACCCCTTTTTTTTTTTGGGAAATTTTT
TTTATGGGGGGATATTTTTTTGGGGGGATTTGTGGTGGGCGGGGGGGCGC
CCAAAAAAAAATAAAAAAAAAAAAAGGAAACAAAAACA
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