EY675113

Overview
NameEY675113
Unique NameEY675113
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length988
Libraries
Library NameType
Sweet orange leaf, infected with CiLVcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of EY675113 vs. ExPASy Swiss-Prot
Match: UB2SC_XENLA (Ubiquitin-conjugating enzyme E2 S-C OS=Xenopus laevis GN=ube2s-C PE=2 SV=1)

HSP 1 Score: 69.3218 bits (168), Expect = 2.263e-17
Identity = 30/87 (34.48%), Postives = 52/87 (59.77%), Query Frame = 2 
Query:  107 KRLMRDFKRLQQDPPAGISGAPQDNNIMLWNAVIFGPDDTPWDGGTFKLTLQFTEDYPNKPPTVRFVSRMFHPNIYADGSICLDILQ 367
            +R+ ++   L  DPP GI   P + +I      I GP+ TP+  G F++ L   +D+P  PP   F++++FHPN+  +G IC+++L+
Sbjct:   14 RRVYKEVSTLTSDPPEGIKIIPNEEDITDVQVHIEGPEGTPYAAGIFRMKLILGKDFPAAPPKGYFLTKIFHPNVSNNGEICVNVLK 100

HSP 2 Score: 41.2022 bits (95), Expect = 2.263e-17
Identity = 21/54 (38.89%), Postives = 27/54 (50.00%), Query Frame = 3 
Query:  366 KIXWSPIYDVAAILTSIQSLLCDPNPNSPANSEAARLFSENKREYNRRVREIVE 527
            K  W     +  +L +I+ LL  PNP S  N EA RL  EN  EY  R + + E
Sbjct:  100 KKDWKAELGIRHVLLTIKCLLIHPNPESALNEEAGRLLLENYEEYASRAKLMTE 153
[Close]
BLAST of EY675113 vs. ExPASy Swiss-Prot
Match: UBE2S_DROMO (Ubiquitin-conjugating enzyme E2 S OS=Drosophila mojavensis GN=GI11085 PE=3 SV=1)

HSP 1 Score: 69.3218 bits (168), Expect = 2.265e-17
Identity = 29/93 (31.18%), Postives = 58/93 (62.37%), Query Frame = 2 
Query:   89 MSTPAKKRLMRDFKRLQQDPPAGISGAPQDNNIMLWNAVIFGPDDTPWDGGTFKLTLQFTEDYPNKPPTVRFVSRMFHPNIYADGSICLDILQ 367
            +S    +++M++ + ++  PP GI     ++++    A+I GP  TP+  G F++ L  ++D+P  PP   F++++FHPN+ A+G IC++ L+
Sbjct:   11 LSPQTIRQVMKELQDMETTPPEGIKVLINESDVTDIQALIDGPAGTPYAVGIFRVKLTLSKDFPQTPPKAYFLTKIFHPNVAANGEICVNTLK 103

HSP 2 Score: 41.2022 bits (95), Expect = 2.265e-17
Identity = 20/54 (37.04%), Postives = 29/54 (53.70%), Query Frame = 3 
Query:  366 KIXWSPIYDVAAILTSIQSLLCDPNPNSPANSEAARLFSENKREYNRRVREIVE 527
            K  W P   +  IL +I+ LL  PNP S  N EA ++  E   +Y++R R + E
Sbjct:  103 KKDWKPDLGIKHILLTIKCLLIVPNPESALNEEAGKMLLERYDDYSQRARMMTE 156
[Close]
BLAST of EY675113 vs. ExPASy Swiss-Prot
Match: UBE2S_DROGR (Ubiquitin-conjugating enzyme E2 S OS=Drosophila grimshawi GN=GH12079 PE=3 SV=1)

HSP 1 Score: 68.5514 bits (166), Expect = 3.827e-17
Identity = 28/93 (30.11%), Postives = 58/93 (62.37%), Query Frame = 2 
Query:   89 MSTPAKKRLMRDFKRLQQDPPAGISGAPQDNNIMLWNAVIFGPDDTPWDGGTFKLTLQFTEDYPNKPPTVRFVSRMFHPNIYADGSICLDILQ 367
            +S    +++M++ + ++  PP GI     ++++    A+I GP  TP+  G F++ L  ++D+P  PP   F++++FHPN+ ++G IC++ L+
Sbjct:   11 LSPQTIRQVMKELQDMESTPPEGIKVLINESDVTDIQALIDGPAGTPYAVGIFRVKLTLSKDFPQTPPKAYFLTKIFHPNVASNGEICVNTLK 103

HSP 2 Score: 41.2022 bits (95), Expect = 3.827e-17
Identity = 20/54 (37.04%), Postives = 29/54 (53.70%), Query Frame = 3 
Query:  366 KIXWSPIYDVAAILTSIQSLLCDPNPNSPANSEAARLFSENKREYNRRVREIVE 527
            K  W P   +  IL +I+ LL  PNP S  N EA ++  E   +Y++R R + E
Sbjct:  103 KKDWKPDLGIKHILLTIKCLLIVPNPESALNEEAGKMLLERYDDYSQRARMMTE 156
[Close]
BLAST of EY675113 vs. ExPASy Swiss-Prot
Match: UBC1_YEAST (Ubiquitin-conjugating enzyme E2-24 kDa OS=Saccharomyces cerevisiae GN=UBC1 PE=1 SV=1)

HSP 1 Score: 68.5514 bits (166), Expect = 4.970e-17
Identity = 33/90 (36.67%), Postives = 58/90 (64.44%), Query Frame = 2 
Query:  107 KRLMRDFKRLQQDPPAGIS-GAPQDNNIMLWNAVIFGPDDTPWDGGTFKLTLQFTEDYPNKPPTVRFVSRMFHPNIYA-DGSICLDILQN 370
            KR+M++ + ++ DP A I+     +++I        GP  TP++GG F + ++   +YP KPP ++F ++++HPNI +  G+ICLDIL+N
Sbjct:    5 KRIMKEIQAVKDDPAAHITLEFVSESDIHHLKGTFLGPPGTPYEGGKFVVDIEVPMEYPFKPPKMQFDTKVYHPNISSVTGAICLDILKN 94

HSP 2 Score: 40.817 bits (94), Expect = 4.970e-17
Identity = 16/47 (34.04%), Postives = 29/47 (61.70%), Query Frame = 3 
Query:  366 KIXWSPIYDVAAILTSIQSLLCDPNPNSPANSEAARLFSENKREYNR 506
            K  WSP+  + + L S+Q+LL  P PN P ++E A+ +  ++  +N+
Sbjct:   93 KNAWSPVITLKSALISLQALLQSPEPNDPQDAEVAQHYLRDRESFNK 139
[Close]
BLAST of EY675113 vs. ExPASy Swiss-Prot
Match: UBE2S_DROVI (Ubiquitin-conjugating enzyme E2 S OS=Drosophila virilis GN=GJ15767 PE=3 SV=1)

HSP 1 Score: 67.781 bits (164), Expect = 6.468e-17
Identity = 28/93 (30.11%), Postives = 57/93 (61.29%), Query Frame = 2 
Query:   89 MSTPAKKRLMRDFKRLQQDPPAGISGAPQDNNIMLWNAVIFGPDDTPWDGGTFKLTLQFTEDYPNKPPTVRFVSRMFHPNIYADGSICLDILQ 367
            +S    +++M++ + ++  PP GI     ++++    A I GP  TP+  G F++ L  ++D+P  PP   F++++FHPN+ ++G IC++ L+
Sbjct:   11 LSPQTIRQVMKELQDMETTPPEGIKVLINESDVTDIQAFIDGPAGTPYAVGVFRVKLTLSKDFPQTPPKAYFLTKIFHPNVASNGEICVNTLK 103

HSP 2 Score: 41.2022 bits (95), Expect = 6.468e-17
Identity = 20/54 (37.04%), Postives = 29/54 (53.70%), Query Frame = 3 
Query:  366 KIXWSPIYDVAAILTSIQSLLCDPNPNSPANSEAARLFSENKREYNRRVREIVE 527
            K  W P   +  IL +I+ LL  PNP S  N EA ++  E   +Y++R R + E
Sbjct:  103 KKDWKPDLGIKHILLTIKCLLIVPNPESALNEEAGKMLLERYDDYSQRARMMTE 156
[Close]
BLAST of EY675113 vs. ExPASy Swiss-Prot
Match: UBE2S_DANRE (Ubiquitin-conjugating enzyme E2 S OS=Danio rerio GN=ube2s PE=2 SV=1)

HSP 1 Score: 68.9366 bits (167), Expect = 3.115e-16
Identity = 30/83 (36.14%), Postives = 50/83 (60.24%), Query Frame = 2 
Query:  119 RDFKRLQQDPPAGISGAPQDNNIMLWNAVIFGPDDTPWDGGTFKLTLQFTEDYPNKPPTVRFVSRMFHPNIYADGSICLDILQ 367
            ++   L  DPP GI   P + +I   +  I GP+ TP+ GG F++ L   +D+P  PP   F++++FHPN+   G IC+++L+
Sbjct:   18 KEVSALAADPPEGIKIYPSEEDITELHTSIEGPEGTPYAGGVFRMRLVLGKDFPAVPPRGYFLTKIFHPNVGHKGEICVNVLK 100

HSP 2 Score: 37.7354 bits (86), Expect = 3.115e-16
Identity = 19/54 (35.19%), Postives = 27/54 (50.00%), Query Frame = 3 
Query:  366 KIXWSPIYDVAAILTSIQSLLCDPNPNSPANSEAARLFSENKREYNRRVREIVE 527
            K  W     +  +L +I+ LL  PNP S  N EA +L  E+ +EY  R   + E
Sbjct:  100 KRDWKAELGLRHVLLTIKCLLIHPNPESALNEEAGKLLLEDYKEYASRAHLLTE 153
[Close]
BLAST of EY675113 vs. ExPASy Swiss-Prot
Match: UBC8_YEAST (Ubiquitin-conjugating enzyme E2-24 kDa OS=Saccharomyces cerevisiae GN=UBC8 PE=1 SV=2)

HSP 1 Score: 62.7734 bits (151), Expect = 3.117e-16
Identity = 25/66 (37.88%), Postives = 47/66 (71.21%), Query Frame = 2 
Query:  176 DNNIMLWNAVIFGPDDTPWDGGTFKLTLQFTEDYPNKPPTVRFVSRMFHPNI-YADGSICLDILQN 370
            ++++  ++    GP DTP++ G ++L ++  ++YP K P++ FV+++FHPNI  A GSICLD++ +
Sbjct:   26 NDSMQEFHVKFLGPKDTPYENGVWRLHVELPDNYPYKSPSIGFVNKIFHPNIDIASGSICLDVINS 91

HSP 2 Score: 43.8986 bits (102), Expect = 3.117e-16
Identity = 21/58 (36.21%), Postives = 36/58 (62.07%), Query Frame = 3 
Query:  375 WSPIYDVAAILT-SIQSLLCDPNPNSPANSEAARLFSENKREYNRRVREIVEQSWTAD 545
            WSP+YD+  I+   I  LL +PN + P N+EAA L   +K+ Y  +++E +++  T +
Sbjct:   93 WSPLYDLINIVEWMIPGLLKEPNGSDPLNNEAATLQLRDKKLYEEKIKEYIDKYATKE 150
[Close]
BLAST of EY675113 vs. ExPASy Swiss-Prot
Match: UE2NL_HUMAN (Putative ubiquitin-conjugating enzyme E2 N-like OS=Homo sapiens GN=UBE2NL PE=5 SV=1)

HSP 1 Score: 70.8626 bits (172), Expect = 4.124e-16
Identity = 34/88 (38.64%), Postives = 56/88 (63.64%), Query Frame = 2 
Query:  110 RLMRDFKRLQQDPPAGISGAPQDNNIMLWNAVIFGPD-DTPWDGGTFKLTLQFTEDYPNKPPTVRFVSRMFHPNIYADGSICLDILQN 370
            R++++ +RL  +P  GI   P ++N   ++ VI G   D+P++GGTFK  L   E+YP   P VRF+++++HPN+     I LDIL++
Sbjct:    7 RIIKETQRLLAEPVPGIKAEPDESNARYFHVVIAGESKDSPFEGGTFKRELLLAEEYPMAAPKVRFMTKIYHPNVDKLERISLDILKD 94

HSP 2 Score: 35.4242 bits (80), Expect = 4.124e-16
Identity = 15/44 (34.09%), Postives = 24/44 (54.55%), Query Frame = 3 
Query:  366 KIXWSPIYDVAAILTSIQSLLCDPNPNSPANSEAARLFSENKRE 497
            K  WSP   +  +L SIQ+LL  PNP+ P  ++    +  N+ +
Sbjct:   93 KDKWSPALQIRTVLLSIQALLNAPNPDDPLANDVVEQWKTNEAQ 136
[Close]
BLAST of EY675113 vs. ExPASy Swiss-Prot
Match: UBC6_ARATH (Ubiquitin-conjugating enzyme E2 6 OS=Arabidopsis thaliana GN=UBC6 PE=2 SV=2)

HSP 1 Score: 61.2326 bits (147), Expect = 1.968e-15
Identity = 28/93 (30.11%), Postives = 56/93 (60.22%), Query Frame = 2 
Query:   89 MSTPAKKRLMRDFKRLQQDPPAGISGAPQDNNIMLWNAVIFGPDDTPWDGGTFKLTLQFTEDYPNKPPTVRFVSRMFHPNI-YADGSICLDIL 364
            M++P+K+R M   K +  D          ++++ ++     GP D+ + GG +K+ ++  E YP K P+V FV++++HPN+  + G++CLD++
Sbjct:    1 MASPSKRREMDMMKLMMSDYKVDTV----NDDLQMFYVTFHGPTDSLYQGGVWKIKVELPEAYPYKSPSVGFVNKIYHPNVDESSGAVCLDVI 89

HSP 2 Score: 42.743 bits (99), Expect = 1.968e-15
Identity = 20/53 (37.74%), Postives = 31/53 (58.49%), Query Frame = 3 
Query:  375 WSPIYDVAAILTS-IQSLLCDPNPNSPANSEAARLFSENKREYNRRVREIVEQ 530
            WSP++D+  +  S +  LL  PNP+ P N EAA L   ++  Y  +V+E  E+
Sbjct:   93 WSPMFDLINVFESFLPQLLLYPNPSDPFNGEAASLLMRDRAAYELKVKEYCEK 145
[Close]
BLAST of EY675113 vs. ExPASy Swiss-Prot
Match: UBC4_ARATH (Ubiquitin-conjugating enzyme E2 4 OS=Arabidopsis thaliana GN=UBC4 PE=2 SV=2)

HSP 1 Score: 57.7658 bits (138), Expect = 3.322e-15
Identity = 29/93 (31.18%), Postives = 52/93 (55.91%), Query Frame = 2 
Query:   89 MSTPAKKRLMRDFKRLQQDPPAGISGAPQDNNIMLWNAVIFGPDDTPWDGGTFKLTLQFTEDYPNKPPTVRFVSRMFHPNI-YADGSICLDIL 364
            MS+P+K+R M   K +  D              + +N    GP D+ + GG +K+ ++  + YP K P+V F+++++HPN+    GS+CLD++
Sbjct:    1 MSSPSKRREMDMMKLMMSDYKVETINDGMQEFYVEFN----GPKDSLYQGGVWKIRVELPDAYPYKSPSVGFITKIYHPNVDELSGSVCLDVI 89

HSP 2 Score: 45.4394 bits (106), Expect = 3.322e-15
Identity = 21/53 (39.62%), Postives = 32/53 (60.38%), Query Frame = 3 
Query:  375 WSPIYDVAAIL-TSIQSLLCDPNPNSPANSEAARLFSENKREYNRRVREIVEQ 530
            WSP++D+  +  T +  LL  PNP+ P N EAA L   ++  Y +RV+E  E+
Sbjct:   93 WSPMFDLVNVFETFLPQLLLYPNPSDPLNGEAAALMMRDRPAYEQRVKEYCEK 145
The following BLAST results are available for this feature:
BLAST of EY675113 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 228
Match NameE-valueIdentityDescription
UB2SC_XENLA2.263e-1734.48Ubiquitin-conjugating enzyme E2 S-C OS=Xenopus lae... [more]
UBE2S_DROMO2.265e-1731.18Ubiquitin-conjugating enzyme E2 S OS=Drosophila mo... [more]
UBE2S_DROGR3.827e-1730.11Ubiquitin-conjugating enzyme E2 S OS=Drosophila gr... [more]
UBC1_YEAST4.970e-1736.67Ubiquitin-conjugating enzyme E2-24 kDa OS=Saccharo... [more]
UBE2S_DROVI6.468e-1730.11Ubiquitin-conjugating enzyme E2 S OS=Drosophila vi... [more]
UBE2S_DANRE3.115e-1636.14Ubiquitin-conjugating enzyme E2 S OS=Danio rerio G... [more]
UBC8_YEAST3.117e-1637.88Ubiquitin-conjugating enzyme E2-24 kDa OS=Saccharo... [more]
UE2NL_HUMAN4.124e-1638.64Putative ubiquitin-conjugating enzyme E2 N-like OS... [more]
UBC6_ARATH1.968e-1530.11Ubiquitin-conjugating enzyme E2 6 OS=Arabidopsis t... [more]
UBC4_ARATH3.322e-1531.18Ubiquitin-conjugating enzyme E2 4 OS=Arabidopsis t... [more]

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Properties
Property NameValue
Genbank descriptionCS00-C1-401-006-C04-CT.F Sweet orange leaf, infected with CiLV Citrus sinensis cDNA, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>EY675113 ID=EY675113; Name=EY675113; organism=Citrus sinensis; type=EST; length=988bp
TTCTTTCTCTAGAAGCTTCTCCTCTCATTCTCCCCCCTTACCCCCTCTGC
AAAAGCCGCTTCTCAGTTTTGCTCTCTAAAACGCCACTATGTCAACACCG
GCGAAGAAGCGGCTGATGCGGGACTTCAAGCGCCTCCAGCAAGATCCACC
CGCCGGAATCAGCGGGGCCCCTCAAGACAACAACATCATGTTATGGAACG
CCGTTATTTTTGGGCCTGATGATACTCCCTGGGACGGAGGTACGTTCAAG
TTGACTCTTCAGTTTACAGAGGATTATCCGAATAAACCTCCAACAGTTCG
GTTCGTTTCACGGATGTTTCATCCAAATATCTATGCGGATGGAAGTATCT
GCCTGGATATATTACAAAATCANGTGGAGTCCTATATATGATGTTGCAGC
TATACTGACCTCCATCCAGTCGCTGCTTTGTGATCCAAATCCAAACTCCC
CTGCAAATTCTGAAGCAGCTCGGCTGTTTAGTGAGAATAAACGAGAGTAC
AACCGAAGAGTGCGTGAAATAGTGGAGCAAAGCTGGACAGCCGACTAAAA
GTCCCATGAAAGCACCGGCCGGTTATGCTGGAGAAATAAAAGATGAAAAC
TGGGGCTGGTGGGGTTGGTTTGGTCAAAATAATGGAACCCGTATTCAATC
GGATTTCAGGTGCATTGTTAATTAAAAAAATCCCCTTCGAATCTTTCCTC
CCCAAAAACCCTGGGTGGAACCCTTGGTTATTTTTCCAACATTTCCCTCT
TCCTTTTCCAAAACTTTCCGGGTAAATGTAATTTTATTGGGGACATTTTG
GTAAGGGGGGTTTTTTGGGGGGCGGGAAAACCCTGTGGATGTTGTTCTTT
AAAAAAACCCCCCCAAAAAAGAAACCCCTTTTTTTTTTTGGGAAATTTTT
TTTATGGGGGGATATTTTTTTGGGGGGATTTGTGGTGGGCGGGGGGGCGC
CCAAAAAAAAATAAAAAAAAAAAAAGGAAACAAAAACA
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