EY675113

Overview
NameEY675113
Unique NameEY675113
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length988
Libraries
Library NameType
Sweet orange leaf, infected with CiLVcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of EY675113 vs. ExPASy Swiss-Prot
Match: UBC5_ARATH (Ubiquitin-conjugating enzyme E2 5 OS=Arabidopsis thaliana GN=UBC5 PE=2 SV=2)

HSP 1 Score: 57.7658 bits (138), Expect = 3.324e-15
Identity = 28/93 (30.11%), Postives = 55/93 (59.14%), Query Frame = 2 
Query:   89 MSTPAKKRLMRDFKRLQQDPPAGISGAPQDNNIMLWNAVIFGPDDTPWDGGTFKLTLQFTEDYPNKPPTVRFVSRMFHPNI-YADGSICLDIL 364
            MS+P+K+R M   K +  D    +     ++ +  +     GP D+ ++GG +K+ ++  + YP K P+V F+++++HPN+    GS+CLD++
Sbjct:    1 MSSPSKRREMDLMKLMMSDYKVEMI----NDGMQEFFVEFSGPKDSIYEGGVWKIRVELPDAYPYKSPSVGFITKIYHPNVDEMSGSVCLDVI 89

HSP 2 Score: 45.4394 bits (106), Expect = 3.324e-15
Identity = 21/53 (39.62%), Postives = 32/53 (60.38%), Query Frame = 3 
Query:  375 WSPIYDVAAIL-TSIQSLLCDPNPNSPANSEAARLFSENKREYNRRVREIVEQ 530
            WSP++D+  +  T +  LL  PNP+ P N EAA L   ++  Y +RV+E  E+
Sbjct:   93 WSPMFDLVNVFETFLPQLLLYPNPSDPLNGEAAALMMRDRPTYEQRVKEYCEK 145
[Close]
BLAST of EY675113 vs. ExPASy Swiss-Prot
Match: UBE2U_MACFA (Ubiquitin-conjugating enzyme E2 U OS=Macaca fascicularis GN=UBE2U PE=2 SV=1)

HSP 1 Score: 68.5514 bits (166), Expect = 5.498e-15
Identity = 34/87 (39.08%), Postives = 49/87 (56.32%), Query Frame = 2 
Query:  113 LMRDFKRLQQDPPAGISGAPQDNNIMLWNAVIFGPDDTPWDGGTFKLTLQFTEDYPNKPPTVRFVSRMFHPNIYA-DGSICLDILQN 370
            L RDF  L+++   GI+  P   ++M W   I G  ++ W G  F+LT+ FT +Y   PP V+FV+  FHPN+    G  C+D L N
Sbjct:    9 LQRDFYDLKENNYKGITARPVSEDMMEWEVEIEGLQNSVWQGLVFQLTIHFTSEYNYAPPVVKFVTIPFHPNVDPHTGQPCIDFLDN 95

HSP 2 Score: 33.8834 bits (76), Expect = 5.498e-15
Identity = 14/42 (33.33%), Postives = 28/42 (66.67%), Query Frame = 3 
Query:  375 WSPIYDVAAILTSIQSLLCDPNPNSPANSEAARLFSENKREY 500
            W+  Y +++IL ++Q +L +P   +P N EAAR+ ++++  Y
Sbjct:   99 WNTNYTLSSILLALQVMLSNPVLENPVNLEAARILTKDESLY 140
[Close]
BLAST of EY675113 vs. ExPASy Swiss-Prot
Match: UBC4_WHEAT (Ubiquitin-conjugating enzyme E2-23 kDa OS=Triticum aestivum GN=UBC4 PE=1 SV=1)

HSP 1 Score: 58.151 bits (139), Expect = 5.615e-15
Identity = 29/93 (31.18%), Postives = 53/93 (56.99%), Query Frame = 2 
Query:   89 MSTPAKKRLMRDFKRLQQDPPAGISGAPQDNNIMLWNAVIFGPDDTPWDGGTFKLTLQFTEDYPNKPPTVRFVSRMFHPNI-YADGSICLDIL 364
            MS+P+K+R M   K +  D    +         + ++    GP D+ + GG +K+ ++ TE YP K P++ F ++++HPN+    GS+CLD++
Sbjct:    1 MSSPSKRREMDLMKLMMSDYKVDMINDGMHEFFVHFH----GPKDSIYQGGVWKVRVELTEAYPYKSPSIGFTNKIYHPNVDEMSGSVCLDVI 89

HSP 2 Score: 44.2838 bits (103), Expect = 5.615e-15
Identity = 21/53 (39.62%), Postives = 30/53 (56.60%), Query Frame = 3 
Query:  375 WSPIYDVAAIL-TSIQSLLCDPNPNSPANSEAARLFSENKREYNRRVREIVEQ 530
            WSP++D+  I    +  LL  PNP+ P N EAA L   +K  Y  +V+E  E+
Sbjct:   93 WSPMFDLVNIFEVFLPQLLLYPNPSDPLNGEAASLMMRDKNAYENKVKEYCER 145
[Close]
BLAST of EY675113 vs. ExPASy Swiss-Prot
Match: UBC12_YEAST (NEDD8-conjugating enzyme UBC12 OS=Saccharomyces cerevisiae GN=UBC12 PE=1 SV=1)

HSP 1 Score: 52.373 bits (124), Expect = 9.475e-15
Identity = 32/93 (34.41%), Postives = 47/93 (50.54%), Query Frame = 2 
Query:  101 AKKRLMRDFKRLQQDPPAGI----SGAPQDNNIMLWNAVIFGPDDTPWDGGTFKLTLQFTEDYPNKPPTVRFVSRMFHPNIYADGSICLDILQ 367
            A+ RL RD   L   P   +    S    D +      VI  PD+  ++ G+    L F E YP +PP V  + ++FHPNI   G++CL+IL+
Sbjct:   28 ARIRLKRDLDSLDLPPTVTLNVITSPDSADRSQSPKLEVIVRPDEGYYNYGSINFNLDFNEVYPIEPPKVVCLKKIFHPNIDLKGNVCLNILR 120

HSP 2 Score: 49.2914 bits (116), Expect = 9.475e-15
Identity = 20/47 (42.55%), Postives = 30/47 (63.83%), Query Frame = 3 
Query:  375 WSPIYDVAAILTSIQSLLCDPNPNSPANSEAARLFSENKREYNRRVR 515
            WSP  D+ +I+T +  L  +PNPN P N +AA+L  E ++E+   VR
Sbjct:  123 WSPALDLQSIITGLLFLFLEPNPNDPLNKDAAKLLCEGEKEFAEAVR 169
[Close]
BLAST of EY675113 vs. ExPASy Swiss-Prot
Match: UBC27_ARATH (Ubiquitin carrier protein E2 27 OS=Arabidopsis thaliana GN=UBC27 PE=2 SV=1)

HSP 1 Score: 68.5514 bits (166), Expect = 1.229e-14
Identity = 34/94 (36.17%), Postives = 58/94 (61.70%), Query Frame = 2 
Query:  116 MRDFKRLQQD--------PPAGISGAPQDNNIMLWNAVIFGPDDTPWDGGTFKLTLQFTEDYPNKPPTVRFVSRMFHPNIYA-DGSICLDILQN 370
            M DF R+Q++          +GI   P+ +N+      I GP  TP++GGTF++ +   + YP +PP ++F ++++HPNI +  G+ICLDIL++
Sbjct:    1 MIDFSRIQKELQDCERNQDSSGIRVCPKSDNLTRLTGTIPGPIGTPYEGGTFQIDITMPDGYPFEPPKMQFSTKVWHPNISSQSGAICLDILKD 94

HSP 2 Score: 32.7278 bits (73), Expect = 1.229e-14
Identity = 15/41 (36.59%), Postives = 21/41 (51.22%), Query Frame = 3 
Query:  366 KIXWSPIYDVAAILTSIQSLLCDPNPNSPANSEAARLFSEN 488
            K  WSP   +   L SIQ+LL  P P  P ++  A  + +N
Sbjct:   93 KDQWSPALTLKTALVSIQALLSAPEPKDPQDAVVAEQYMKN 133
[Close]
BLAST of EY675113 vs. ExPASy Swiss-Prot
Match: UBE2H_MOUSE (Ubiquitin-conjugating enzyme E2 H OS=Mus musculus GN=Ube2h PE=2 SV=1)

HSP 1 Score: 58.5362 bits (140), Expect = 1.601e-14
Identity = 22/53 (41.51%), Postives = 39/53 (73.58%), Query Frame = 2 
Query:  209 FGPDDTPWDGGTFKLTLQFTEDYPNKPPTVRFVSRMFHPNI-YADGSICLDIL 364
            +GP  TP++GG +K+ +   + YP K P++ F++++FHPNI  A G++CLD++
Sbjct:   39 YGPQGTPYEGGVWKVRVDLPDKYPFKSPSIGFMNKIFHPNIDEASGTVCLDVI 91

HSP 2 Score: 42.3578 bits (98), Expect = 1.601e-14
Identity = 19/66 (28.79%), Postives = 35/66 (53.03%), Query Frame = 3 
Query:  375 WSPIYDVAAILTS-IQSLLCDPNPNSPANSEAARLFSENKREYNRRVREIVEQSWTAD*KSHESTG 569
            W+ +YD+  I  S +  LL  PNP  P N +AA ++     EY ++++E +++  T +    +  G
Sbjct:   95 WTALYDLTNIFESFLPQLLAYPNPIDPLNGDAAAMYLHRPEEYKQKIKEYIQKYATEEALKEQEEG 160
[Close]
BLAST of EY675113 vs. ExPASy Swiss-Prot
Match: UBE2H_HUMAN (Ubiquitin-conjugating enzyme E2 H OS=Homo sapiens GN=UBE2H PE=1 SV=1)

HSP 1 Score: 58.5362 bits (140), Expect = 1.601e-14
Identity = 22/53 (41.51%), Postives = 39/53 (73.58%), Query Frame = 2 
Query:  209 FGPDDTPWDGGTFKLTLQFTEDYPNKPPTVRFVSRMFHPNI-YADGSICLDIL 364
            +GP  TP++GG +K+ +   + YP K P++ F++++FHPNI  A G++CLD++
Sbjct:   39 YGPQGTPYEGGVWKVRVDLPDKYPFKSPSIGFMNKIFHPNIDEASGTVCLDVI 91

HSP 2 Score: 42.3578 bits (98), Expect = 1.601e-14
Identity = 19/66 (28.79%), Postives = 35/66 (53.03%), Query Frame = 3 
Query:  375 WSPIYDVAAILTS-IQSLLCDPNPNSPANSEAARLFSENKREYNRRVREIVEQSWTAD*KSHESTG 569
            W+ +YD+  I  S +  LL  PNP  P N +AA ++     EY ++++E +++  T +    +  G
Sbjct:   95 WTALYDLTNIFESFLPQLLAYPNPIDPLNGDAAAMYLHRPEEYKQKIKEYIQKYATEEALKEQEEG 160
[Close]
BLAST of EY675113 vs. ExPASy Swiss-Prot
Match: UBE2H_BOVIN (Ubiquitin-conjugating enzyme E2 H OS=Bos taurus GN=UBE2H PE=2 SV=1)

HSP 1 Score: 58.5362 bits (140), Expect = 1.601e-14
Identity = 22/53 (41.51%), Postives = 39/53 (73.58%), Query Frame = 2 
Query:  209 FGPDDTPWDGGTFKLTLQFTEDYPNKPPTVRFVSRMFHPNI-YADGSICLDIL 364
            +GP  TP++GG +K+ +   + YP K P++ F++++FHPNI  A G++CLD++
Sbjct:   39 YGPQGTPYEGGVWKVRVDLPDKYPFKSPSIGFMNKIFHPNIDEASGTVCLDVI 91

HSP 2 Score: 42.3578 bits (98), Expect = 1.601e-14
Identity = 19/66 (28.79%), Postives = 35/66 (53.03%), Query Frame = 3 
Query:  375 WSPIYDVAAILTS-IQSLLCDPNPNSPANSEAARLFSENKREYNRRVREIVEQSWTAD*KSHESTG 569
            W+ +YD+  I  S +  LL  PNP  P N +AA ++     EY ++++E +++  T +    +  G
Sbjct:   95 WTALYDLTNIFESFLPQLLAYPNPIDPLNGDAAAMYLHRPEEYKQKIKEYIQKYATEEALKEQEEG 160
[Close]
BLAST of EY675113 vs. ExPASy Swiss-Prot
Match: UBE2U_HUMAN (Ubiquitin-conjugating enzyme E2 U OS=Homo sapiens GN=UBE2U PE=1 SV=1)

HSP 1 Score: 67.0106 bits (162), Expect = 2.036e-14
Identity = 33/87 (37.93%), Postives = 49/87 (56.32%), Query Frame = 2 
Query:  113 LMRDFKRLQQDPPAGISGAPQDNNIMLWNAVIFGPDDTPWDGGTFKLTLQFTEDYPNKPPTVRFVSRMFHPNIYA-DGSICLDILQN 370
            L RDF  L+++   GI+  P   ++M W   I G  ++ W G  F+LT+ FT +Y   PP V+F++  FHPN+    G  C+D L N
Sbjct:    9 LHRDFCDLKENNYKGITAKPVSEDMMEWEVEIEGLQNSVWQGLVFQLTIHFTSEYNYAPPVVKFITIPFHPNVDPHTGQPCIDFLDN 95

HSP 2 Score: 33.4982 bits (75), Expect = 2.036e-14
Identity = 15/47 (31.91%), Postives = 29/47 (61.70%), Query Frame = 3 
Query:  375 WSPIYDVAAILTSIQSLLCDPNPNSPANSEAARLFSENKREYNRRVR 515
            W+  Y +++IL ++Q +L +P   +P N EAAR+  +++  Y   +R
Sbjct:   99 WNTNYTLSSILLALQVMLSNPVLENPVNLEAARILVKDESLYRTILR 145
[Close]
BLAST of EY675113 vs. ExPASy Swiss-Prot
Match: UBC12_DROME (NEDD8-conjugating enzyme Ubc12 OS=Drosophila melanogaster GN=CG7375 PE=1 SV=1)

HSP 1 Score: 55.4546 bits (132), Expect = 2.083e-14
Identity = 27/92 (29.35%), Postives = 53/92 (57.61%), Query Frame = 2 
Query:   92 STPAKKRLMRDFKRLQQDPPAGISGAPQDNNIMLWNAVIFGPDDTPWDGGTFKLTLQFTEDYPNKPPTVRFVSRMFHPNIYADGSICLDILQ 367
            ++ A+ R+ +D   L   P    +  P  N+++ +  +I  PD+  +  G F    +   +YP++PP V+  ++++HPNI  DG++CL+IL+
Sbjct:   24 ASAAQLRIQKDINELNL-PNTCATDFPDPNDLLNFKLII-SPDEGFYRDGRFVFNFRVGSNYPHEPPKVKCATQVYHPNIDLDGNVCLNILR 113

HSP 2 Score: 45.0542 bits (105), Expect = 2.083e-14
Identity = 16/48 (33.33%), Postives = 30/48 (62.50%), Query Frame = 3 
Query:  375 WSPIYDVAAILTSIQSLLCDPNPNSPANSEAARLFSENKREYNRRVRE 518
            W+P+ ++ +I+  +Q L  +PNP  P N EAA +   N+R++   V++
Sbjct:  116 WNPVLNINSIVYGLQFLFLEPNPEDPLNKEAADVLQTNRRQFENNVKK 163
The following BLAST results are available for this feature:
BLAST of EY675113 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 228
Match NameE-valueIdentityDescription
UBC5_ARATH3.324e-1530.11Ubiquitin-conjugating enzyme E2 5 OS=Arabidopsis t... [more]
UBE2U_MACFA5.498e-1539.08Ubiquitin-conjugating enzyme E2 U OS=Macaca fascic... [more]
UBC4_WHEAT5.615e-1531.18Ubiquitin-conjugating enzyme E2-23 kDa OS=Triticum... [more]
UBC12_YEAST9.475e-1534.41NEDD8-conjugating enzyme UBC12 OS=Saccharomyces ce... [more]
UBC27_ARATH1.229e-1436.17Ubiquitin carrier protein E2 27 OS=Arabidopsis tha... [more]
UBE2H_MOUSE1.601e-1441.51Ubiquitin-conjugating enzyme E2 H OS=Mus musculus ... [more]
UBE2H_HUMAN1.601e-1441.51Ubiquitin-conjugating enzyme E2 H OS=Homo sapiens ... [more]
UBE2H_BOVIN1.601e-1441.51Ubiquitin-conjugating enzyme E2 H OS=Bos taurus GN... [more]
UBE2U_HUMAN2.036e-1437.93Ubiquitin-conjugating enzyme E2 U OS=Homo sapiens ... [more]
UBC12_DROME2.083e-1429.35NEDD8-conjugating enzyme Ubc12 OS=Drosophila melan... [more]

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Properties
Property NameValue
Genbank descriptionCS00-C1-401-006-C04-CT.F Sweet orange leaf, infected with CiLV Citrus sinensis cDNA, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>EY675113 ID=EY675113; Name=EY675113; organism=Citrus sinensis; type=EST; length=988bp
TTCTTTCTCTAGAAGCTTCTCCTCTCATTCTCCCCCCTTACCCCCTCTGC
AAAAGCCGCTTCTCAGTTTTGCTCTCTAAAACGCCACTATGTCAACACCG
GCGAAGAAGCGGCTGATGCGGGACTTCAAGCGCCTCCAGCAAGATCCACC
CGCCGGAATCAGCGGGGCCCCTCAAGACAACAACATCATGTTATGGAACG
CCGTTATTTTTGGGCCTGATGATACTCCCTGGGACGGAGGTACGTTCAAG
TTGACTCTTCAGTTTACAGAGGATTATCCGAATAAACCTCCAACAGTTCG
GTTCGTTTCACGGATGTTTCATCCAAATATCTATGCGGATGGAAGTATCT
GCCTGGATATATTACAAAATCANGTGGAGTCCTATATATGATGTTGCAGC
TATACTGACCTCCATCCAGTCGCTGCTTTGTGATCCAAATCCAAACTCCC
CTGCAAATTCTGAAGCAGCTCGGCTGTTTAGTGAGAATAAACGAGAGTAC
AACCGAAGAGTGCGTGAAATAGTGGAGCAAAGCTGGACAGCCGACTAAAA
GTCCCATGAAAGCACCGGCCGGTTATGCTGGAGAAATAAAAGATGAAAAC
TGGGGCTGGTGGGGTTGGTTTGGTCAAAATAATGGAACCCGTATTCAATC
GGATTTCAGGTGCATTGTTAATTAAAAAAATCCCCTTCGAATCTTTCCTC
CCCAAAAACCCTGGGTGGAACCCTTGGTTATTTTTCCAACATTTCCCTCT
TCCTTTTCCAAAACTTTCCGGGTAAATGTAATTTTATTGGGGACATTTTG
GTAAGGGGGGTTTTTTGGGGGGCGGGAAAACCCTGTGGATGTTGTTCTTT
AAAAAAACCCCCCCAAAAAAGAAACCCCTTTTTTTTTTTGGGAAATTTTT
TTTATGGGGGGATATTTTTTTGGGGGGATTTGTGGTGGGCGGGGGGGCGC
CCAAAAAAAAATAAAAAAAAAAAAAGGAAACAAAAACA
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