EY675113 | Citrus Genome Database

Overview


Name	EY675113
Unique Name	EY675113
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	988

Libraries


Library Name	Type
Sweet orange leaf, infected with CiLV	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Homology

[Close]

BLAST of EY675113 vs. ExPASy Swiss-Prot
Match: UBC1_MIMIV (Probable ubiquitin-conjugating enzyme E2 L460 OS=Acanthamoeba polyphaga mimivirus GN=UBC PE=3 SV=1)

HSP 1 Score: 57.3806 bits (137), Expect = 2.102e-14
Identity = 24/64 (37.50%), Postives = 40/64 (62.50%), Query Frame = 2

Query:  176 DNNIMLWNAVIFGPDDTPWDGGTFKLTLQFTEDYPNKPPTVRFVSRMFHPNIYADGSICLDILQ 367
            D NI LW   I GP+++ ++   F+L ++ + DYP   P V+F++ + H N+   G ICL+IL+
Sbjct:   33 DENIFLWKVNIKGPENSLYENYNFELEIELSNDYPYSSPKVKFITPIQHMNVNDKGDICLNILK 96

HSP 2 Score: 43.1282 bits (100), Expect = 2.102e-14
Identity = 20/54 (37.04%), Postives = 32/54 (59.26%), Query Frame = 3

Query:  357 IYYKIXWSPIYDVAAILTSIQSLLCDPNPNSPANSEAARLFSENKREYNRRVRE 518
            I  K  W+   ++ +I+ SI  LL  PNP  P NSE A L+  +K  Y++++R+
Sbjct:   94 ILKKDGWNASLNIISIIWSIIVLLDQPNPEDPFNSELASLYRNDKLSYDKKIRD 147

[Close]

BLAST of EY675113 vs. ExPASy Swiss-Prot
Match: UBE2Z_XENLA (Ubiquitin-conjugating enzyme E2 Z OS=Xenopus laevis GN=ube2z PE=2 SV=2)

HSP 1 Score: 70.0922 bits (170), Expect = 4.470e-14
Identity = 35/100 (35.00%), Postives = 54/100 (54.00%), Query Frame = 2

Query:   80 NATMSTPAKKRLMRDFKRLQQDPPAGISGAPQDNNIMLWNAVIFGPDDTPWDGGTFKLTLQFTEDYPNKPPTVRFVSR-----MFHPNIYADGSICLDIL 364
            N   ST    R+ RD   + ++PP G+   P  +++   +A+I GP DTP++GG F    +   DYP  PP V+ ++       F+PN Y +G +CL IL
Sbjct:   52 NERASTQCVLRIKRDIMSIYKEPPPGMFVVPDPHDMTKIHALITGPFDTPYEGGFFLFLFRCPPDYPIHPPRVKLMTTGNNTVRFNPNFYRNGKVCLSIL 151

HSP 2 Score: 29.261 bits (64), Expect = 4.470e-14
Identity = 16/48 (33.33%), Postives = 26/48 (54.17%), Query Frame = 3

Query:  375 WSPIYDVAAILTSIQSLLCD-PNPNSPANSEAARLFSENKREYNRRVR 515
            WSP   ++++L SIQSL+ + P  N P   +     S + + YN  +R
Sbjct:  159 WSPAQSLSSVLISIQSLMTENPYHNEPGFEQERH--SGDSKNYNECIR 204

[Close]

BLAST of EY675113 vs. ExPASy Swiss-Prot
Match: UBC12_DICDI (NEDD8-conjugating enzyme Ubc12 OS=Dictyostelium discoideum GN=ube2m PE=3 SV=1)

HSP 1 Score: 53.5286 bits (127), Expect = 4.520e-14
Identity = 28/92 (30.43%), Postives = 48/92 (52.17%), Query Frame = 2

Query:   92 STPAKKRLMRDFKRLQQDPPAGISGAPQDNNIMLWNAVIFGPDDTPWDGGTFKLTLQFTEDYPNKPPTVRFVSRMFHPNIYADGSICLDILQ 367
            + PA+ R  +D   ++   P G + + +D N +L   +   P D  +   TF+ T+     YP  PP V   + ++HPNI  +G +CL+IL+
Sbjct:   75 ANPAELRAQKDIDEMEV--PTGCAVSFKDTNDILNFNLSITPTDGLYQSATFQFTINIPSTYPYDPPKVHCDTLVYHPNIDLEGHVCLNILR 164

HSP 2 Score: 45.8246 bits (107), Expect = 4.520e-14
Identity = 15/48 (31.25%), Postives = 30/48 (62.50%), Query Frame = 3

Query:  375 WSPIYDVAAILTSIQSLLCDPNPNSPANSEAARLFSENKREYNRRVRE 518
            W P+ ++  ++  + +L  +PNP+ P N +AA+L  +NK+ +   VR+
Sbjct:  167 WMPVLNIGTVIFGLMTLFLEPNPDDPLNKDAAQLMIDNKKTFEANVRQ 214

[Close]

BLAST of EY675113 vs. ExPASy Swiss-Prot
Match: UBC16_SCHPO (Ubiquitin-conjugating enzyme E2 16 OS=Schizosaccharomyces pombe GN=ubc16 PE=2 SV=2)

HSP 1 Score: 65.4698 bits (158), Expect = 4.605e-14
Identity = 33/90 (36.67%), Postives = 54/90 (60.00%), Query Frame = 2

Query:  107 KRLMRDFKRLQQDPPAGIS--GAPQDNNIMLWNAVIFGPDDTPWDGGTFKLTLQFTEDYPNKPPTVRFVSRMFHPNI-YADGSICLDILQ 367
            KRL +++   Q    +G+       D ++  W AVI GP +TP++GG + L +   E YP  PP+V F +++ HPNI + +G +C+DIL+
Sbjct:    6 KRLHKEYASFQAGDISGLLLLKPVTDVDMFHWKAVIEGPTETPYEGGQWVLDIHVHEGYPISPPSVYFQTKIVHPNISWTNGEVCMDILK 95

HSP 2 Score: 33.8834 bits (76), Expect = 4.605e-14
Identity = 19/51 (37.25%), Postives = 30/51 (58.82%), Query Frame = 3

Query:  366 KIXWSPIYDVAAILTSIQSLLCDPNPNSPANSEAARLF-SENKREYNRRVR 515
            K  WSP + + +   +I SLL + + +SP N +AA+L  + +K  YN  VR
Sbjct:   95 KTHWSPAWSLQSACLAIISLLSNYDASSPLNVDAAKLLRTGDKTAYNSLVR 145

[Close]

BLAST of EY675113 vs. ExPASy Swiss-Prot
Match: UB2L6_MOUSE (Ubiquitin/ISG15-conjugating enzyme E2 L6 OS=Mus musculus GN=Ube2l6 PE=2 SV=3)

HSP 1 Score: 70.8626 bits (172), Expect = 4.619e-14
Identity = 30/91 (32.97%), Postives = 57/91 (62.64%), Query Frame = 2

Query:  101 AKKRLMRDFKRLQQD-PPAGISGAPQDNNIMLWNAVIFGPDDTPWDGGTFKLTLQFTEDYPNKPPTVRFVSRMFHPNIYADGSICLDILQN 370
            A KR+ ++ + L ++ PP     +  D N+++W+ ++  PD  P+    F++ + F  +YP KPPT+RF ++++HPN+  DG +CL ++ N
Sbjct:    3 ASKRVAKELESLSKELPPYLRQLSSDDANVLVWHMLLL-PDQLPYGLKAFQVRIDFPREYPFKPPTLRFTTKIYHPNVREDGLVCLPLISN 92

HSP 2 Score: 28.4906 bits (62), Expect = 4.619e-14
Identity = 12/48 (25.00%), Postives = 21/48 (43.75%), Query Frame = 3

Query:  375 WSPIYDVAAILTSIQSLLCDPNPNSPANSEAARLFSENKREYNRRVRE 518
            W P      +L ++  L+  PN   P   E A L ++N   + ++  E
Sbjct:   95 WKPYTKPYQVLEALNVLVSKPNLEEPVRLELADLLTQNPEMFRKKAEE 142

[Close]

BLAST of EY675113 vs. ExPASy Swiss-Prot
Match: UB2L3_MOUSE (Ubiquitin-conjugating enzyme E2 L3 OS=Mus musculus GN=Ube2l3 PE=2 SV=1)

HSP 1 Score: 65.855 bits (159), Expect = 1.013e-13
Identity = 28/89 (31.46%), Postives = 52/89 (58.43%), Query Frame = 2

Query:  101 AKKRLMRDFKRLQQDPPAGISGAPQDN-NIMLWNAVIFGPDDTPWDGGTFKLTLQFTEDYPNKPPTVRFVSRMFHPNIYADGSICLDIL 364
            A +RLM++ + +++           D  N++ W  +I  PD+ P+D G F++ + F  +YP KPP + F ++++HPNI   G +CL ++
Sbjct:    3 ASRRLMKELEEIRKCGMKNFRNIQVDEANLLTWQGLIV-PDNPPYDKGAFRIEINFPAEYPFKPPKITFKTKIYHPNIDEKGQVCLPVI 90

HSP 2 Score: 32.3426 bits (72), Expect = 1.013e-13
Identity = 11/52 (21.15%), Postives = 28/52 (53.85%), Query Frame = 3

Query:  375 WSPIYDVAAILTSIQSLLCDPNPNSPANSEAARLFSENKREYNRRVREIVEQ 530
            W P      ++ S+ +L+ DP P  P  ++ A  +S++++++ +   E  ++
Sbjct:   95 WKPATKTDQVIQSLIALVNDPQPEHPLRADLAEEYSKDRKKFCKNAEEFTKK 146

[Close]

BLAST of EY675113 vs. ExPASy Swiss-Prot
Match: UB2L3_HUMAN (Ubiquitin-conjugating enzyme E2 L3 OS=Homo sapiens GN=UBE2L3 PE=1 SV=1)

HSP 1 Score: 65.855 bits (159), Expect = 1.013e-13
Identity = 28/89 (31.46%), Postives = 52/89 (58.43%), Query Frame = 2

Query:  101 AKKRLMRDFKRLQQDPPAGISGAPQDN-NIMLWNAVIFGPDDTPWDGGTFKLTLQFTEDYPNKPPTVRFVSRMFHPNIYADGSICLDIL 364
            A +RLM++ + +++           D  N++ W  +I  PD+ P+D G F++ + F  +YP KPP + F ++++HPNI   G +CL ++
Sbjct:    3 ASRRLMKELEEIRKCGMKNFRNIQVDEANLLTWQGLIV-PDNPPYDKGAFRIEINFPAEYPFKPPKITFKTKIYHPNIDEKGQVCLPVI 90

HSP 2 Score: 32.3426 bits (72), Expect = 1.013e-13
Identity = 11/52 (21.15%), Postives = 28/52 (53.85%), Query Frame = 3

Query:  375 WSPIYDVAAILTSIQSLLCDPNPNSPANSEAARLFSENKREYNRRVREIVEQ 530
            W P      ++ S+ +L+ DP P  P  ++ A  +S++++++ +   E  ++
Sbjct:   95 WKPATKTDQVIQSLIALVNDPQPEHPLRADLAEEYSKDRKKFCKNAEEFTKK 146

[Close]

BLAST of EY675113 vs. ExPASy Swiss-Prot
Match: UB2L3_BOVIN (Ubiquitin-conjugating enzyme E2 L3 OS=Bos taurus GN=UBE2L3 PE=2 SV=1)

HSP 1 Score: 65.855 bits (159), Expect = 1.013e-13
Identity = 28/89 (31.46%), Postives = 52/89 (58.43%), Query Frame = 2

Query:  101 AKKRLMRDFKRLQQDPPAGISGAPQDN-NIMLWNAVIFGPDDTPWDGGTFKLTLQFTEDYPNKPPTVRFVSRMFHPNIYADGSICLDIL 364
            A +RLM++ + +++           D  N++ W  +I  PD+ P+D G F++ + F  +YP KPP + F ++++HPNI   G +CL ++
Sbjct:    3 ASRRLMKELEEIRKCGMKNFRNIQVDEANLLTWQGLIV-PDNPPYDKGAFRIEINFPAEYPFKPPKITFKTKIYHPNIDEKGQVCLPVI 90

HSP 2 Score: 32.3426 bits (72), Expect = 1.013e-13
Identity = 11/52 (21.15%), Postives = 28/52 (53.85%), Query Frame = 3

Query:  375 WSPIYDVAAILTSIQSLLCDPNPNSPANSEAARLFSENKREYNRRVREIVEQ 530
            W P      ++ S+ +L+ DP P  P  ++ A  +S++++++ +   E  ++
Sbjct:   95 WKPATKTDQVIQSLIALVNDPQPEHPLRADLAEEYSKDRKKFCKNAEEFTKK 146

[Close]

BLAST of EY675113 vs. ExPASy Swiss-Prot
Match: UB2L3_PONAB (Ubiquitin-conjugating enzyme E2 L3 OS=Pongo abelii GN=UBE2L3 PE=2 SV=1)

HSP 1 Score: 65.4698 bits (158), Expect = 1.316e-13
Identity = 28/89 (31.46%), Postives = 52/89 (58.43%), Query Frame = 2

Query:  101 AKKRLMRDFKRLQQDPPAGISGAPQDN-NIMLWNAVIFGPDDTPWDGGTFKLTLQFTEDYPNKPPTVRFVSRMFHPNIYADGSICLDIL 364
            A +RLM++ + +++           D  N++ W  +I  PD+ P+D G F++ + F  +YP KPP + F ++++HPNI   G +CL ++
Sbjct:    3 ASRRLMKELEEIRKCGMKNFRNIRVDEANLLTWQGLIV-PDNPPYDKGAFRIEINFPAEYPFKPPKITFKTKIYHPNIDEKGQVCLPVI 90

HSP 2 Score: 32.3426 bits (72), Expect = 1.316e-13
Identity = 11/52 (21.15%), Postives = 28/52 (53.85%), Query Frame = 3

Query:  375 WSPIYDVAAILTSIQSLLCDPNPNSPANSEAARLFSENKREYNRRVREIVEQ 530
            W P      ++ S+ +L+ DP P  P  ++ A  +S++++++ +   E  ++
Sbjct:   95 WKPATKTDQVIQSLIALVNDPQPEHPLRADLAEEYSKDRKKFCKNAEEFTKK 146

[Close]

BLAST of EY675113 vs. ExPASy Swiss-Prot
Match: UBE2Z_XENTR (Ubiquitin-conjugating enzyme E2 Z OS=Xenopus tropicalis GN=ube2z PE=2 SV=2)

HSP 1 Score: 68.1662 bits (165), Expect = 1.654e-13
Identity = 34/100 (34.00%), Postives = 53/100 (53.00%), Query Frame = 2

Query:   80 NATMSTPAKKRLMRDFKRLQQDPPAGISGAPQDNNIMLWNAVIFGPDDTPWDGGTFKLTLQFTEDYPNKPPTVRFVSR-----MFHPNIYADGSICLDIL 364
            N   S     R+ RD   + ++PP G+   P  +++   +A+I GP DTP++GG F    +   DYP  PP V+ ++       F+PN Y +G +CL IL
Sbjct:   52 NERASNQCVLRIKRDIMSIYKEPPPGMFVVPDPHDMTKIHALITGPFDTPYEGGFFLFLFRCPPDYPIHPPRVKLMTTGNNTVRFNPNFYRNGKVCLSIL 151

HSP 2 Score: 29.261 bits (64), Expect = 1.654e-13
Identity = 16/48 (33.33%), Postives = 26/48 (54.17%), Query Frame = 3

Query:  375 WSPIYDVAAILTSIQSLLCD-PNPNSPANSEAARLFSENKREYNRRVR 515
            WSP   ++++L SIQSL+ + P  N P   +     S + + YN  +R
Sbjct:  159 WSPAQSLSSVLISIQSLMTENPYHNEPGFEQERH--SGDSKNYNECIR 204

The following BLAST results are available for this feature:

BLAST of EY675113 vs. ExPASy Swiss-Prot

BLAST of EY675113 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 228


Match Name	E-value	Identity	Description
UBC1_MIMIV	2.102e-14	37.50	Probable ubiquitin-conjugating enzyme E2 L460 OS=A...	[more]
UBE2Z_XENLA	4.470e-14	35.00	Ubiquitin-conjugating enzyme E2 Z OS=Xenopus laevi...	[more]
UBC12_DICDI	4.520e-14	30.43	NEDD8-conjugating enzyme Ubc12 OS=Dictyostelium di...	[more]
UBC16_SCHPO	4.605e-14	36.67	Ubiquitin-conjugating enzyme E2 16 OS=Schizosaccha...	[more]
UB2L6_MOUSE	4.619e-14	32.97	Ubiquitin/ISG15-conjugating enzyme E2 L6 OS=Mus mu...	[more]
UB2L3_MOUSE	1.013e-13	31.46	Ubiquitin-conjugating enzyme E2 L3 OS=Mus musculus...	[more]
UB2L3_HUMAN	1.013e-13	31.46	Ubiquitin-conjugating enzyme E2 L3 OS=Homo sapiens...	[more]
UB2L3_BOVIN	1.013e-13	31.46	Ubiquitin-conjugating enzyme E2 L3 OS=Bos taurus G...	[more]
UB2L3_PONAB	1.316e-13	31.46	Ubiquitin-conjugating enzyme E2 L3 OS=Pongo abelii...	[more]
UBE2Z_XENTR	1.654e-13	34.00	Ubiquitin-conjugating enzyme E2 Z OS=Xenopus tropi...	[more]

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Properties


Property Name	Value
Genbank description	CS00-C1-401-006-C04-CT.F Sweet orange leaf, infected with CiLV Citrus sinensis cDNA, mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>EY675113 ID=EY675113; Name=EY675113; organism=Citrus sinensis; type=EST; length=988bp
TTCTTTCTCTAGAAGCTTCTCCTCTCATTCTCCCCCCTTACCCCCTCTGC
AAAAGCCGCTTCTCAGTTTTGCTCTCTAAAACGCCACTATGTCAACACCG
GCGAAGAAGCGGCTGATGCGGGACTTCAAGCGCCTCCAGCAAGATCCACC
CGCCGGAATCAGCGGGGCCCCTCAAGACAACAACATCATGTTATGGAACG
CCGTTATTTTTGGGCCTGATGATACTCCCTGGGACGGAGGTACGTTCAAG
TTGACTCTTCAGTTTACAGAGGATTATCCGAATAAACCTCCAACAGTTCG
GTTCGTTTCACGGATGTTTCATCCAAATATCTATGCGGATGGAAGTATCT
GCCTGGATATATTACAAAATCANGTGGAGTCCTATATATGATGTTGCAGC
TATACTGACCTCCATCCAGTCGCTGCTTTGTGATCCAAATCCAAACTCCC
CTGCAAATTCTGAAGCAGCTCGGCTGTTTAGTGAGAATAAACGAGAGTAC
AACCGAAGAGTGCGTGAAATAGTGGAGCAAAGCTGGACAGCCGACTAAAA
GTCCCATGAAAGCACCGGCCGGTTATGCTGGAGAAATAAAAGATGAAAAC
TGGGGCTGGTGGGGTTGGTTTGGTCAAAATAATGGAACCCGTATTCAATC
GGATTTCAGGTGCATTGTTAATTAAAAAAATCCCCTTCGAATCTTTCCTC
CCCAAAAACCCTGGGTGGAACCCTTGGTTATTTTTCCAACATTTCCCTCT
TCCTTTTCCAAAACTTTCCGGGTAAATGTAATTTTATTGGGGACATTTTG
GTAAGGGGGGTTTTTTGGGGGGCGGGAAAACCCTGTGGATGTTGTTCTTT
AAAAAAACCCCCCCAAAAAAGAAACCCCTTTTTTTTTTTGGGAAATTTTT
TTTATGGGGGGATATTTTTTTGGGGGGATTTGTGGTGGGCGGGGGGGCGC
CCAAAAAAAAATAAAAAAAAAAAAAGGAAACAAAAACA