EY675113

Overview
NameEY675113
Unique NameEY675113
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length988
Libraries
Library NameType
Sweet orange leaf, infected with CiLVcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of EY675113 vs. ExPASy Swiss-Prot
Match: UBC21_CAEEL (Probable ubiquitin-conjugating enzyme E2 21 OS=Caenorhabditis elegans GN=ubc-21 PE=2 SV=2)

HSP 1 Score: 63.1586 bits (152), Expect = 2.173e-13
Identity = 41/113 (36.28%), Postives = 60/113 (53.10%), Query Frame = 2 
Query:   89 MSTPAKKRLMRDFKRLQQDPP---AGISGAPQDNNIMLWNAVIFGPDDTPWDGGTFKLTLQFTEDYPNKPPTV---------------RFVSRMFHPNIYAD-GSICLDILQN 370
            MS  A  R+ R  K +        AGI    ++NN+M     I GP+ TP+ GGTF++ +   E YP +PP V               +FV+R++HPNI +  G+ICLDIL++
Sbjct:   15 MSNLALARVTRKCKEVANASDITEAGIHVEIKENNLMDIKGFIKGPEGTPYAGGTFEIKVDIPEHYPFEPPKVTEIIFHIRAFEYIQAKFVTRIWHPNISSQTGTICLDILKD 127

HSP 2 Score: 33.8834 bits (76), Expect = 2.173e-13
Identity = 13/41 (31.71%), Postives = 23/41 (56.10%), Query Frame = 3 
Query:  366 KIXWSPIYDVAAILTSIQSLLCDPNPNSPANSEAARLFSEN 488
            K  W+    +  +L S+Q++LC P P+ P ++  A+ F  N
Sbjct:  126 KDKWTASLTLRTVLLSLQAMLCSPEPSDPQDAVVAKQFINN 166
[Close]
BLAST of EY675113 vs. ExPASy Swiss-Prot
Match: UBC12_YARLI (NEDD8-conjugating enzyme UBC12 OS=Yarrowia lipolytica GN=UBC12 PE=3 SV=1)

HSP 1 Score: 54.6842 bits (130), Expect = 2.858e-13
Identity = 27/91 (29.67%), Postives = 52/91 (57.14%), Query Frame = 2 
Query:   95 TPAKKRLMRDFKRLQQDPPAGISGAPQDNNIMLWNAVIFGPDDTPWDGGTFKLTLQFTEDYPNKPPTVRFVSRMFHPNIYADGSICLDILQ 367
            T A+ R+ +D   L   P    +  P   +IM +   +  PD+  + GG F+ +     ++P++PP V+ + +++HPNI  +G++CL+IL+
Sbjct:   23 TAAQLRVQKDLSELSL-PSTMKTHFPSAEDIMNFELTV-RPDEGFYQGGEFRFSFYVNPNFPHEPPKVKCLQKVYHPNIDLEGNVCLNILR 111

HSP 2 Score: 41.9726 bits (97), Expect = 2.858e-13
Identity = 15/47 (31.91%), Postives = 27/47 (57.45%), Query Frame = 3 
Query:  375 WSPIYDVAAILTSIQSLLCDPNPNSPANSEAARLFSENKREYNRRVR 515
            W P+  + A++  +Q L  +PN + P N +AA   + N+ E+ R V+
Sbjct:  114 WKPVLSLNAVMIGLQYLFLEPNASDPLNKDAAHQMTANREEFKRNVK 160
[Close]
BLAST of EY675113 vs. ExPASy Swiss-Prot
Match: UBC6_CANGA (Ubiquitin-conjugating enzyme E2 6 OS=Candida glabrata GN=UBC6 PE=3 SV=1)

HSP 1 Score: 76.2554 bits (186), Expect = 3.159e-13
Identity = 32/70 (45.71%), Postives = 47/70 (67.14%), Query Frame = 2 
Query:  101 AKKRLMRDFKRLQQDPPAGISGAPQDNNIMLWNAVIFGPDDTPWDGGTFKLTLQFTEDYPNKPPTVRFVS 310
            A+KRL +++K + ++PP  I   P + NI+ W+ VI GP DTP+DGG +  TL F  DYP KPP +R ++
Sbjct:    6 AQKRLTKEYKMMVENPPPFIIARPNEENILEWHYVISGPPDTPYDGGQYHGTLTFPSDYPYKPPAIRMIT 75
[Close]
BLAST of EY675113 vs. ExPASy Swiss-Prot
Match: UBE2Z_DANRE (Ubiquitin-conjugating enzyme E2 Z OS=Danio rerio GN=ube2z PE=2 SV=2)

HSP 1 Score: 68.1662 bits (165), Expect = 3.620e-13
Identity = 32/90 (35.56%), Postives = 51/90 (56.67%), Query Frame = 2 
Query:  110 RLMRDFKRLQQDPPAGISGAPQDNNIMLWNAVIFGPDDTPWDGGTFKLTLQFTEDYPNKPPTVRFVSR-----MFHPNIYADGSICLDIL 364
            R+ RD   + ++PP G+   P  +++   +A+I GP DTP++GG F    +   DYP  PP V+ ++       F+PN Y +G +CL IL
Sbjct:   75 RIKRDIMSIYKEPPPGMFVVPDPHDMTKIHALITGPFDTPYEGGFFLFLFRCPPDYPIHPPRVKLITTGHNTVRFNPNFYRNGKVCLSIL 164

HSP 2 Score: 28.1054 bits (61), Expect = 3.620e-13
Identity = 15/48 (31.25%), Postives = 25/48 (52.08%), Query Frame = 3 
Query:  375 WSPIYDVAAILTSIQSLLCD-PNPNSPANSEAARLFSENKREYNRRVR 515
            WSP   ++++L SIQSL+ + P  N P   +       + + YN  +R
Sbjct:  172 WSPAQSISSVLISIQSLMTENPYHNEPGFEQERH--PGDSKNYNECIR 217
[Close]
BLAST of EY675113 vs. ExPASy Swiss-Prot
Match: UBC6_ASHGO (Ubiquitin-conjugating enzyme E2 6 OS=Ashbya gossypii GN=UBC6 PE=3 SV=1)

HSP 1 Score: 71.2478 bits (173), Expect = 3.659e-13
Identity = 30/73 (41.10%), Postives = 49/73 (67.12%), Query Frame = 2 
Query:   92 STPAKKRLMRDFKRLQQDPPAGISGAPQDNNIMLWNAVIFGPDDTPWDGGTFKLTLQFTEDYPNKPPTVRFVS 310
            S  A KRL +++K + ++PP  I  AP+++NI++W+ VI GP +TP++ G +  TL F  DYP  PP +R ++
Sbjct:    3 SRQAYKRLSKEYKMMTENPPPYIVAAPKEDNILVWHYVITGPPETPYEDGQYHGTLVFPNDYPFNPPAIRMLT 75

HSP 2 Score: 25.0238 bits (53), Expect = 3.659e-13
Identity = 14/59 (23.73%), Postives = 27/59 (45.76%), Query Frame = 3 
Query:  336 RMEVSAWIYYKIXWSPIYDVAAILTSIQSLLCDPNPN---SPANSEAARLFSENKREYN 503
            R+ +S   Y+   W+P + VA ILT + S +     +     +     R ++   +E+N
Sbjct:   85 RLCLSMSDYHPDTWNPSWSVATILTGLLSFMTTDESSIGTMESTDSTKRKYAAKSKEHN 143
[Close]
BLAST of EY675113 vs. ExPASy Swiss-Prot
Match: UBC12_SCHPO (NEDD8-conjugating enzyme ubc12 OS=Schizosaccharomyces pombe GN=ubc12 PE=2 SV=1)

HSP 1 Score: 59.6918 bits (143), Expect = 3.713e-13
Identity = 31/96 (32.29%), Postives = 59/96 (61.46%), Query Frame = 2 
Query:   80 NATMSTPAKKRLMRDFKRLQQDPPAGISGAPQDNNIMLWNAVIFGPDDTPWDGGTFKLTLQFTEDYPNKPPTVRFVSRMFHPNIYADGSICLDILQ 367
            NA+  +PA+ R+ +D   L+   P+ +S +  D   +    +   PD+  + GG FK  +Q  ++YP+ PP V+ +++++HPNI  +G++CL+IL+
Sbjct:   17 NASGISPAQIRIQKDVTDLEI--PSTMSTSWPDPIKLNVLHLEIRPDEGYYKGGKFKFRIQIDDNYPHDPPKVKCLNKIYHPNIDIEGNVCLNILR 110

HSP 2 Score: 36.5798 bits (83), Expect = 3.713e-13
Identity = 16/47 (34.04%), Postives = 26/47 (55.32%), Query Frame = 3 
Query:  375 WSPIYDVAAILTSIQSLLCDPNPNSPANSEAARLFSENKREYNRRVR 515
            W+P+ ++ +IL  +Q L   PN   P N EAA    ++ + +  RVR
Sbjct:  113 WNPVLNLNSILVGLQFLFLSPNAEDPLNKEAAADLHKDPQGFASRVR 159
[Close]
BLAST of EY675113 vs. ExPASy Swiss-Prot
Match: UBC12_XENTR (NEDD8-conjugating enzyme Ubc12 OS=Xenopus tropicalis GN=ube2m PE=2 SV=1)

HSP 1 Score: 52.373 bits (124), Expect = 6.253e-13
Identity = 26/92 (28.26%), Postives = 51/92 (55.43%), Query Frame = 2 
Query:   92 STPAKKRLMRDFKRLQQDPPAGISGAPQDNNIMLWNAVIFGPDDTPWDGGTFKLTLQFTEDYPNKPPTVRFVSRMFHPNIYADGSICLDILQ 367
            ++ A+ R+ +D   L       I  +  D+  +L   ++  PD+  + GG F  + +  + YP+ PP V+  + ++HPNI  +G++CL+IL+
Sbjct:   27 ASAAQLRIQKDINELNLPKTCEIEFSDHDD--LLNFKLVICPDEGFYKGGKFVFSFKVGQGYPHDPPKVKCETMVYHPNIDLEGNVCLNILR 116

HSP 2 Score: 43.1282 bits (100), Expect = 6.253e-13
Identity = 16/47 (34.04%), Postives = 27/47 (57.45%), Query Frame = 3 
Query:  375 WSPIYDVAAILTSIQSLLCDPNPNSPANSEAARLFSENKREYNRRVR 515
            W P+  + +I+  +Q L  +PNP  P N EAA +   N+R + + V+
Sbjct:  119 WKPVLTINSIIYGLQYLFLEPNPEDPLNKEAAEVLQNNRRLFEQNVQ 165
[Close]
BLAST of EY675113 vs. ExPASy Swiss-Prot
Match: UBC12_XENLA (NEDD8-conjugating enzyme Ubc12 OS=Xenopus laevis GN=ube2m PE=2 SV=1)

HSP 1 Score: 51.9878 bits (123), Expect = 8.123e-13
Identity = 26/92 (28.26%), Postives = 51/92 (55.43%), Query Frame = 2 
Query:   92 STPAKKRLMRDFKRLQQDPPAGISGAPQDNNIMLWNAVIFGPDDTPWDGGTFKLTLQFTEDYPNKPPTVRFVSRMFHPNIYADGSICLDILQ 367
            ++ A+ R+ +D   L       I  +  D+  +L   ++  PD+  + GG F  + +  + YP+ PP V+  + ++HPNI  +G++CL+IL+
Sbjct:   27 ASAAQLRIQKDITELNLPKTCEIEFSDHDD--LLNFKLVICPDEGFYKGGKFVFSFKVGQGYPHDPPKVKCETMVYHPNIDLEGNVCLNILR 116

HSP 2 Score: 43.1282 bits (100), Expect = 8.123e-13
Identity = 16/47 (34.04%), Postives = 27/47 (57.45%), Query Frame = 3 
Query:  375 WSPIYDVAAILTSIQSLLCDPNPNSPANSEAARLFSENKREYNRRVR 515
            W P+  + +I+  +Q L  +PNP  P N EAA +   N+R + + V+
Sbjct:  119 WKPVLTINSIIYGLQYLFLEPNPEDPLNKEAAEVLQNNRRLFEQNVQ 165
[Close]
BLAST of EY675113 vs. ExPASy Swiss-Prot
Match: UBC8_SCHPO (Ubiquitin-conjugating enzyme E2 8 OS=Schizosaccharomyces pombe GN=ubc8 PE=2 SV=1)

HSP 1 Score: 59.6918 bits (143), Expect = 1.055e-12
Identity = 25/64 (39.06%), Postives = 43/64 (67.19%), Query Frame = 2 
Query:  176 DNNIMLWNAVIFGPDDTPWDGGTFKLTLQFTEDYPNKPPTVRFVSRMFHPNI-YADGSICLDIL 364
            ++N+  +     GP +TP+ GG +K+ ++   +YP K P++ FV+R+FHPNI    GS+CLD++
Sbjct:   26 NDNMQEFYVRFHGPSETPYSGGIWKVHVELPSEYPWKSPSIGFVNRIFHPNIDELSGSVCLDVI 89

HSP 2 Score: 35.039 bits (79), Expect = 1.055e-12
Identity = 18/51 (35.29%), Postives = 26/51 (50.98%), Query Frame = 3 
Query:  375 WSPIYDVAAIL-TSIQSLLCDPNPNSPANSEAARLFSENKREYNRRVREIV 524
            WSP++D+  I    +  LL  PN + P N EAA L       Y  +VR+ +
Sbjct:   93 WSPMFDMINIFEVFLPQLLRYPNASDPLNGEAAALLLREPNTYYAKVRDYI 143
[Close]
BLAST of EY675113 vs. ExPASy Swiss-Prot
Match: UBC12_MOUSE (NEDD8-conjugating enzyme Ubc12 OS=Mus musculus GN=Ube2m PE=2 SV=1)

HSP 1 Score: 51.6026 bits (122), Expect = 1.055e-12
Identity = 26/92 (28.26%), Postives = 51/92 (55.43%), Query Frame = 2 
Query:   92 STPAKKRLMRDFKRLQQDPPAGISGAPQDNNIMLWNAVIFGPDDTPWDGGTFKLTLQFTEDYPNKPPTVRFVSRMFHPNIYADGSICLDILQ 367
            ++ A+ R+ +D   L       IS +  D+  +L   ++  PD+  +  G F  + +  + YP+ PP V+  + ++HPNI  +G++CL+IL+
Sbjct:   27 ASAAQLRIQKDINELNLPKTCDISFSDPDD--LLNFKLVICPDEGFYKSGKFVFSFKVGQGYPHDPPKVKCETMVYHPNIDLEGNVCLNILR 116

HSP 2 Score: 43.1282 bits (100), Expect = 1.055e-12
Identity = 16/47 (34.04%), Postives = 27/47 (57.45%), Query Frame = 3 
Query:  375 WSPIYDVAAILTSIQSLLCDPNPNSPANSEAARLFSENKREYNRRVR 515
            W P+  + +I+  +Q L  +PNP  P N EAA +   N+R + + V+
Sbjct:  119 WKPVLTINSIIYGLQYLFLEPNPEDPLNKEAAEVLQNNRRLFEQNVQ 165
The following BLAST results are available for this feature:
BLAST of EY675113 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 228
Match NameE-valueIdentityDescription
UBC21_CAEEL2.173e-1336.28Probable ubiquitin-conjugating enzyme E2 21 OS=Cae... [more]
UBC12_YARLI2.858e-1329.67NEDD8-conjugating enzyme UBC12 OS=Yarrowia lipolyt... [more]
UBC6_CANGA3.159e-1345.71Ubiquitin-conjugating enzyme E2 6 OS=Candida glabr... [more]
UBE2Z_DANRE3.620e-1335.56Ubiquitin-conjugating enzyme E2 Z OS=Danio rerio G... [more]
UBC6_ASHGO3.659e-1341.10Ubiquitin-conjugating enzyme E2 6 OS=Ashbya gossyp... [more]
UBC12_SCHPO3.713e-1332.29NEDD8-conjugating enzyme ubc12 OS=Schizosaccharomy... [more]
UBC12_XENTR6.253e-1328.26NEDD8-conjugating enzyme Ubc12 OS=Xenopus tropical... [more]
UBC12_XENLA8.123e-1328.26NEDD8-conjugating enzyme Ubc12 OS=Xenopus laevis G... [more]
UBC8_SCHPO1.055e-1239.06Ubiquitin-conjugating enzyme E2 8 OS=Schizosacchar... [more]
UBC12_MOUSE1.055e-1228.26NEDD8-conjugating enzyme Ubc12 OS=Mus musculus GN=... [more]

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Properties
Property NameValue
Genbank descriptionCS00-C1-401-006-C04-CT.F Sweet orange leaf, infected with CiLV Citrus sinensis cDNA, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>EY675113 ID=EY675113; Name=EY675113; organism=Citrus sinensis; type=EST; length=988bp
TTCTTTCTCTAGAAGCTTCTCCTCTCATTCTCCCCCCTTACCCCCTCTGC
AAAAGCCGCTTCTCAGTTTTGCTCTCTAAAACGCCACTATGTCAACACCG
GCGAAGAAGCGGCTGATGCGGGACTTCAAGCGCCTCCAGCAAGATCCACC
CGCCGGAATCAGCGGGGCCCCTCAAGACAACAACATCATGTTATGGAACG
CCGTTATTTTTGGGCCTGATGATACTCCCTGGGACGGAGGTACGTTCAAG
TTGACTCTTCAGTTTACAGAGGATTATCCGAATAAACCTCCAACAGTTCG
GTTCGTTTCACGGATGTTTCATCCAAATATCTATGCGGATGGAAGTATCT
GCCTGGATATATTACAAAATCANGTGGAGTCCTATATATGATGTTGCAGC
TATACTGACCTCCATCCAGTCGCTGCTTTGTGATCCAAATCCAAACTCCC
CTGCAAATTCTGAAGCAGCTCGGCTGTTTAGTGAGAATAAACGAGAGTAC
AACCGAAGAGTGCGTGAAATAGTGGAGCAAAGCTGGACAGCCGACTAAAA
GTCCCATGAAAGCACCGGCCGGTTATGCTGGAGAAATAAAAGATGAAAAC
TGGGGCTGGTGGGGTTGGTTTGGTCAAAATAATGGAACCCGTATTCAATC
GGATTTCAGGTGCATTGTTAATTAAAAAAATCCCCTTCGAATCTTTCCTC
CCCAAAAACCCTGGGTGGAACCCTTGGTTATTTTTCCAACATTTCCCTCT
TCCTTTTCCAAAACTTTCCGGGTAAATGTAATTTTATTGGGGACATTTTG
GTAAGGGGGGTTTTTTGGGGGGCGGGAAAACCCTGTGGATGTTGTTCTTT
AAAAAAACCCCCCCAAAAAAGAAACCCCTTTTTTTTTTTGGGAAATTTTT
TTTATGGGGGGATATTTTTTTGGGGGGATTTGTGGTGGGCGGGGGGGCGC
CCAAAAAAAAATAAAAAAAAAAAAAGGAAACAAAAACA
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