EY675113

Overview
NameEY675113
Unique NameEY675113
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length988
Libraries
Library NameType
Sweet orange leaf, infected with CiLVcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of EY675113 vs. ExPASy Swiss-Prot
Match: UBC7_ARATH (Ubiquitin-conjugating enzyme E2 7 OS=Arabidopsis thaliana GN=UBC7 PE=2 SV=1)

HSP 1 Score: 88.9669 bits (219), Expect = 2.984e-25
Identity = 41/93 (44.09%), Postives = 57/93 (61.29%), Query Frame = 2 
Query:   89 MSTPAKKRLMRDFKRLQQDPPAGIS-GAPQDNNIMLWNAVIFGPDDTPWDGGTFKLTLQFTEDYPNKPPTVRFVSRMFHPNIYADGSICLDIL 364
            M++ A   L +  K L + P  G S G   + NI  W+  I GP DT ++GG F   + F ++YPN PPTVRF S M+HPN+Y+DG +C+ IL
Sbjct:    1 MASQASLLLQKQLKDLCKHPVDGFSAGLVDEKNIFEWSVTIIGPPDTLYEGGFFNAIMTFPQNYPNSPPTVRFTSDMWHPNVYSDGRVCISIL 93

HSP 2 Score: 48.1358 bits (113), Expect = 2.984e-25
Identity = 21/53 (39.62%), Postives = 35/53 (66.04%), Query Frame = 3 
Query:  375 WSPIYDVAAILTSIQSLLCDPNPNSPANSEAARLFSENKREYNRRVREIVEQS 533
            W+P++ V +I+ SI S+L  PN  SPAN EAA+ + + + E+ ++V   V +S
Sbjct:  110 WTPVHTVESIMLSIISMLSGPNDESPANVEAAKEWRDKRDEFKKKVSRCVRKS 162
[Close]
BLAST of EY675113 vs. ExPASy Swiss-Prot
Match: UBC20_ARATH (Ubiquitin carrier protein E2 20 OS=Arabidopsis thaliana GN=UBC20 PE=2 SV=1)

HSP 1 Score: 83.9593 bits (206), Expect = 6.556e-25
Identity = 39/88 (44.32%), Postives = 54/88 (61.36%), Query Frame = 2 
Query:  107 KRLMRDFKRLQQDPPAGISGAPQDNNIMLWNAVIFGPDDTPWDGGTFKLTLQFTEDYPNKPPTVRFVSRMFHPNIYADGSICLDILQN 370
            KRL  +   L      GIS  P+++NI  W   I G  DT ++G  ++L+L F+ DYP KPP V+F +  FHPN+   G+ICLDILQ+
Sbjct:   38 KRLQSELMGLMMGGGPGISAFPEEDNIFCWKGTITGSKDTVFEGTEYRLSLSFSNDYPFKPPKVKFETCCFHPNVDVYGNICLDILQD 125

HSP 2 Score: 51.9878 bits (123), Expect = 6.556e-25
Identity = 26/49 (53.06%), Postives = 36/49 (73.47%), Query Frame = 3 
Query:  375 WSPIYDVAAILTSIQSLLCDPNPNSPANSEAARLFSENKREYNRRVREI 521
            WS  YDV  IL SIQSLL +PN +SP N++AA+L+S N+ EY + V ++
Sbjct:  127 WSSAYDVRTILLSIQSLLGEPNISSPLNTQAAQLWS-NQEEYRKMVEKL 174
[Close]
BLAST of EY675113 vs. ExPASy Swiss-Prot
Match: UBE2C_DICDI (Probable ubiquitin-conjugating enzyme E2 C OS=Dictyostelium discoideum GN=ube2c PE=3 SV=1)

HSP 1 Score: 85.5001 bits (210), Expect = 6.604e-25
Identity = 40/88 (45.45%), Postives = 55/88 (62.50%), Query Frame = 2 
Query:  107 KRLMRDFKRLQQDPPAGISGAPQDNNIMLWNAVIFGPDDTPWDGGTFKLTLQFTEDYPNKPPTVRFVSRMFHPNIYADGSICLDILQN 370
            KRL  +   L   P  GIS  P  +NI  W   I G  DT ++   FK++L+F  DYP KPPTV+F ++ FHPN+  +G+ICLDIL++
Sbjct:    9 KRLQSELMNLMMGPSPGISAFPDGDNIFNWIGTIQGAKDTVYEQMEFKMSLKFPTDYPYKPPTVKFETQCFHPNVDNNGNICLDILKD 96

HSP 2 Score: 50.447 bits (119), Expect = 6.604e-25
Identity = 26/47 (55.32%), Postives = 34/47 (72.34%), Query Frame = 3 
Query:  366 KIXWSPIYDVAAILTSIQSLLCDPNPNSPANSEAARLFSENKREYNR 506
            K  WSP+Y+V ++L SIQSLL +PN  SP NS AA L+S N+ EY +
Sbjct:   95 KDKWSPVYNVRSLLISIQSLLGEPNNESPLNSYAASLWS-NQDEYKK 140
[Close]
BLAST of EY675113 vs. ExPASy Swiss-Prot
Match: UBC5_YEAST (Ubiquitin-conjugating enzyme E2-16 kDa OS=Saccharomyces cerevisiae GN=UBC5 PE=1 SV=1)

HSP 1 Score: 94.7449 bits (234), Expect = 6.619e-25
Identity = 40/90 (44.44%), Postives = 60/90 (66.67%), Query Frame = 2 
Query:  101 AKKRLMRDFKRLQQDPPAGISGAPQDNNIMLWNAVIFGPDDTPWDGGTFKLTLQFTEDYPNKPPTVRFVSRMFHPNIYADGSICLDILQN 370
            + KR+ ++   L +DPPA  S  P  +++  W A I GP D+P+ GG F L++ F  DYP KPP V F ++++HPNI + G+ICLDIL++
Sbjct:    3 SSKRIAKELSDLGRDPPASCSAGPVGDDLYHWQASIMGPSDSPYAGGVFFLSIHFPTDYPFKPPKVNFTTKIYHPNINSSGNICLDILKD 92

HSP 2 Score: 41.2022 bits (95), Expect = 6.619e-25
Identity = 19/51 (37.25%), Postives = 29/51 (56.86%), Query Frame = 3 
Query:  366 KIXWSPIYDVAAILTSIQSLLCDPNPNSPANSEAARLFSENKREYNRRVRE 518
            K  WSP   ++ +L SI SLL D NP+ P   E A+++  +K +Y    +E
Sbjct:   91 KDQWSPALTLSKVLLSICSLLTDANPDDPLVPEIAQIYKTDKAKYEATAKE 141
[Close]
BLAST of EY675113 vs. ExPASy Swiss-Prot
Match: UBC4_YEAST (Ubiquitin-conjugating enzyme E2 4 OS=Saccharomyces cerevisiae GN=UBC4 PE=1 SV=1)

HSP 1 Score: 94.7449 bits (234), Expect = 1.121e-24
Identity = 39/90 (43.33%), Postives = 61/90 (67.78%), Query Frame = 2 
Query:  101 AKKRLMRDFKRLQQDPPAGISGAPQDNNIMLWNAVIFGPDDTPWDGGTFKLTLQFTEDYPNKPPTVRFVSRMFHPNIYADGSICLDILQN 370
            + KR+ ++   L++DPP   S  P  +++  W A I GP D+P+ GG F L++ F  DYP KPP + F ++++HPNI A+G+ICLDIL++
Sbjct:    3 SSKRIAKELSDLERDPPTSCSAGPVGDDLYHWQASIMGPADSPYAGGVFFLSIHFPTDYPFKPPKISFTTKIYHPNINANGNICLDILKD 92

HSP 2 Score: 40.4318 bits (93), Expect = 1.121e-24
Identity = 19/51 (37.25%), Postives = 28/51 (54.90%), Query Frame = 3 
Query:  366 KIXWSPIYDVAAILTSIQSLLCDPNPNSPANSEAARLFSENKREYNRRVRE 518
            K  WSP   ++ +L SI SLL D NP+ P   E A ++  ++ +Y    RE
Sbjct:   91 KDQWSPALTLSKVLLSICSLLTDANPDDPLVPEIAHIYKTDRPKYEATARE 141
[Close]
BLAST of EY675113 vs. ExPASy Swiss-Prot
Match: UBC13_ARATH (Ubiquitin-conjugating enzyme E2 13 OS=Arabidopsis thaliana GN=UBC13 PE=2 SV=1)

HSP 1 Score: 85.1149 bits (209), Expect = 1.888e-24
Identity = 40/93 (43.01%), Postives = 56/93 (60.22%), Query Frame = 2 
Query:   89 MSTPAKKRLMRDFKRLQQDPPAGIS-GAPQDNNIMLWNAVIFGPDDTPWDGGTFKLTLQFTEDYPNKPPTVRFVSRMFHPNIYADGSICLDIL 364
            M++ A   L +  K L + P  G S G   + NI  W+  I GP DT ++GG F   + F ++YPN PPTVRF S ++HPN+Y DG +C+ IL
Sbjct:    1 MNSQACLLLQKQLKDLCKHPVDGFSAGLVDEKNIFEWSVTIIGPPDTLYEGGFFYAIMSFPQNYPNSPPTVRFTSDIWHPNVYPDGRVCISIL 93

HSP 2 Score: 49.2914 bits (116), Expect = 1.888e-24
Identity = 22/53 (41.51%), Postives = 35/53 (66.04%), Query Frame = 3 
Query:  375 WSPIYDVAAILTSIQSLLCDPNPNSPANSEAARLFSENKREYNRRVREIVEQS 533
            W+P++ V +I+ SI S+L  PN  SPAN EAA+ + E + E+ ++V   V +S
Sbjct:  110 WTPVHTVESIMLSIISMLSGPNDESPANVEAAKEWREKRDEFKKKVSRCVRKS 162
[Close]
BLAST of EY675113 vs. ExPASy Swiss-Prot
Match: UBC14_ARATH (Ubiquitin-conjugating enzyme E2 14 OS=Arabidopsis thaliana GN=UBC14 PE=2 SV=1)

HSP 1 Score: 82.8037 bits (203), Expect = 4.162e-24
Identity = 37/85 (43.53%), Postives = 51/85 (60.00%), Query Frame = 2 
Query:  113 LMRDFKRLQQDPPAGIS-GAPQDNNIMLWNAVIFGPDDTPWDGGTFKLTLQFTEDYPNKPPTVRFVSRMFHPNIYADGSICLDIL 364
            L +  K L + P  G S G   + N+  W+  I GP DT ++GG F   + F E+YP  PPTV F S M+HPN+Y+DG +C+ IL
Sbjct:   10 LQKQLKDLCKKPVDGFSAGLVDEKNVFQWSVSIMGPPDTLYEGGFFNAIMSFPENYPVSPPTVTFTSEMWHPNVYSDGKVCISIL 94

HSP 2 Score: 50.447 bits (119), Expect = 4.162e-24
Identity = 22/53 (41.51%), Postives = 36/53 (67.92%), Query Frame = 3 
Query:  375 WSPIYDVAAILTSIQSLLCDPNPNSPANSEAARLFSENKREYNRRVREIVEQS 533
            W+P++ V +I+ SI S+L  PN  SPAN EAA+ + +N+ E+ ++V   V +S
Sbjct:  111 WTPVHTVESIVLSIISMLSGPNDESPANVEAAKEWRDNRAEFRKKVSRCVRRS 163
[Close]
BLAST of EY675113 vs. ExPASy Swiss-Prot
Match: UBC19_ARATH (Ubiquitin carrier protein E2 19 OS=Arabidopsis thaliana GN=UBC19 PE=1 SV=1)

HSP 1 Score: 80.8777 bits (198), Expect = 5.397e-24
Identity = 38/88 (43.18%), Postives = 53/88 (60.23%), Query Frame = 2 
Query:  107 KRLMRDFKRLQQDPPAGISGAPQDNNIMLWNAVIFGPDDTPWDGGTFKLTLQFTEDYPNKPPTVRFVSRMFHPNIYADGSICLDILQN 370
            KRL  +   L      GIS  P+++NI  W   I G  DT ++G  ++L+L F+ DYP K P V+F +  FHPN+   G+ICLDILQ+
Sbjct:   39 KRLQSELMGLMMGADPGISAFPEEDNIFCWKGTITGSKDTVFEGTEYRLSLTFSNDYPFKSPKVKFETCCFHPNVDLYGNICLDILQD 126

HSP 2 Score: 51.9878 bits (123), Expect = 5.397e-24
Identity = 26/49 (53.06%), Postives = 36/49 (73.47%), Query Frame = 3 
Query:  375 WSPIYDVAAILTSIQSLLCDPNPNSPANSEAARLFSENKREYNRRVREI 521
            WS  YDV  IL SIQSLL +PN +SP N++AA+L+S N+ EY + V ++
Sbjct:  128 WSSAYDVRTILLSIQSLLGEPNISSPLNNQAAQLWS-NQEEYRKMVEKL 175
[Close]
BLAST of EY675113 vs. ExPASy Swiss-Prot
Match: UBC7_WHEAT (Ubiquitin-conjugating enzyme E2 7 OS=Triticum aestivum GN=UBC7 PE=2 SV=1)

HSP 1 Score: 82.0333 bits (201), Expect = 5.412e-24
Identity = 32/66 (48.48%), Postives = 43/66 (65.15%), Query Frame = 2 
Query:  164 GAPQDNNIMLWNAVIFGPDDTPWDGGTFKLTLQFTEDYPNKPPTVRFVSRMFHPNIYADGSICLDI 361
            G   D+N+  W   I GP +T +DGG F   + F ++YPN PPTVRF S M+HPN+Y DG +C+ I
Sbjct:   30 GFVDDSNVFEWQVTIIGPPETLYDGGYFNAIMSFPQNYPNSPPTVRFTSEMWHPNVYPDGRVCISI 95

HSP 2 Score: 50.8322 bits (120), Expect = 5.412e-24
Identity = 23/53 (43.40%), Postives = 36/53 (67.92%), Query Frame = 3 
Query:  375 WSPIYDVAAILTSIQSLLCDPNPNSPANSEAARLFSENKREYNRRVREIVEQS 533
            W+P++ V +I+ SI S+L  PN  SPAN EAA+ + E + E+ ++VR  V +S
Sbjct:  112 WTPVHTVESIVLSIISMLSSPNDESPANIEAAKDWREKQDEFKKKVRRAVRKS 164
[Close]
BLAST of EY675113 vs. ExPASy Swiss-Prot
Match: UBC7_CAEEL (Probable ubiquitin-conjugating enzyme E2 7 OS=Caenorhabditis elegans GN=ubc-7 PE=1 SV=1)

HSP 1 Score: 81.6481 bits (200), Expect = 7.055e-24
Identity = 37/89 (41.57%), Postives = 56/89 (62.92%), Query Frame = 2 
Query:  107 KRLMRDFKRLQQDPPAGIS-GAPQDNNIMLWNAVIFGPDDTPWDGGTFKLTLQFTEDYPNKPPTVRFVSRMFHPNIYADGSICLDILQN 370
            K+ + D +R+  D   G S G   DN+I  W  ++ GP DT ++GG FK  L F  DYP KPP ++F+S ++HPNI  +G++C+ IL +
Sbjct:    9 KKQLADMRRVPVD---GFSAGLVDDNDIYKWEVLVIGPPDTLYEGGFFKAILDFPRDYPQKPPKMKFISEIWHPNIDKEGNVCISILHD 94

HSP 2 Score: 50.8322 bits (120), Expect = 7.055e-24
Identity = 23/53 (43.40%), Postives = 35/53 (66.04%), Query Frame = 3 
Query:  375 WSPIYDVAAILTSIQSLLCDPNPNSPANSEAARLFSENKREYNRRVREIVEQS 533
            W P++ V  IL S+ S+L DPN  SPAN +AA++  EN  E+ ++V + V +S
Sbjct:  109 WLPVHTVETILLSVISMLTDPNFESPANVDAAKMQRENYAEFKKKVAQCVRRS 161
The following BLAST results are available for this feature:
BLAST of EY675113 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 228
Match NameE-valueIdentityDescription
UBC7_ARATH2.984e-2544.09Ubiquitin-conjugating enzyme E2 7 OS=Arabidopsis t... [more]
UBC20_ARATH6.556e-2544.32Ubiquitin carrier protein E2 20 OS=Arabidopsis tha... [more]
UBE2C_DICDI6.604e-2545.45Probable ubiquitin-conjugating enzyme E2 C OS=Dict... [more]
UBC5_YEAST6.619e-2544.44Ubiquitin-conjugating enzyme E2-16 kDa OS=Saccharo... [more]
UBC4_YEAST1.121e-2443.33Ubiquitin-conjugating enzyme E2 4 OS=Saccharomyces... [more]
UBC13_ARATH1.888e-2443.01Ubiquitin-conjugating enzyme E2 13 OS=Arabidopsis ... [more]
UBC14_ARATH4.162e-2443.53Ubiquitin-conjugating enzyme E2 14 OS=Arabidopsis ... [more]
UBC19_ARATH5.397e-2443.18Ubiquitin carrier protein E2 19 OS=Arabidopsis tha... [more]
UBC7_WHEAT5.412e-2448.48Ubiquitin-conjugating enzyme E2 7 OS=Triticum aest... [more]
UBC7_CAEEL7.055e-2441.57Probable ubiquitin-conjugating enzyme E2 7 OS=Caen... [more]

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Properties
Property NameValue
Genbank descriptionCS00-C1-401-006-C04-CT.F Sweet orange leaf, infected with CiLV Citrus sinensis cDNA, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>EY675113 ID=EY675113; Name=EY675113; organism=Citrus sinensis; type=EST; length=988bp
TTCTTTCTCTAGAAGCTTCTCCTCTCATTCTCCCCCCTTACCCCCTCTGC
AAAAGCCGCTTCTCAGTTTTGCTCTCTAAAACGCCACTATGTCAACACCG
GCGAAGAAGCGGCTGATGCGGGACTTCAAGCGCCTCCAGCAAGATCCACC
CGCCGGAATCAGCGGGGCCCCTCAAGACAACAACATCATGTTATGGAACG
CCGTTATTTTTGGGCCTGATGATACTCCCTGGGACGGAGGTACGTTCAAG
TTGACTCTTCAGTTTACAGAGGATTATCCGAATAAACCTCCAACAGTTCG
GTTCGTTTCACGGATGTTTCATCCAAATATCTATGCGGATGGAAGTATCT
GCCTGGATATATTACAAAATCANGTGGAGTCCTATATATGATGTTGCAGC
TATACTGACCTCCATCCAGTCGCTGCTTTGTGATCCAAATCCAAACTCCC
CTGCAAATTCTGAAGCAGCTCGGCTGTTTAGTGAGAATAAACGAGAGTAC
AACCGAAGAGTGCGTGAAATAGTGGAGCAAAGCTGGACAGCCGACTAAAA
GTCCCATGAAAGCACCGGCCGGTTATGCTGGAGAAATAAAAGATGAAAAC
TGGGGCTGGTGGGGTTGGTTTGGTCAAAATAATGGAACCCGTATTCAATC
GGATTTCAGGTGCATTGTTAATTAAAAAAATCCCCTTCGAATCTTTCCTC
CCCAAAAACCCTGGGTGGAACCCTTGGTTATTTTTCCAACATTTCCCTCT
TCCTTTTCCAAAACTTTCCGGGTAAATGTAATTTTATTGGGGACATTTTG
GTAAGGGGGGTTTTTTGGGGGGCGGGAAAACCCTGTGGATGTTGTTCTTT
AAAAAAACCCCCCCAAAAAAGAAACCCCTTTTTTTTTTTGGGAAATTTTT
TTTATGGGGGGATATTTTTTTGGGGGGATTTGTGGTGGGCGGGGGGGCGC
CCAAAAAAAAATAAAAAAAAAAAAAGGAAACAAAAACA
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