EY675113

Overview
NameEY675113
Unique NameEY675113
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length988
Libraries
Library NameType
Sweet orange leaf, infected with CiLVcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of EY675113 vs. ExPASy Swiss-Prot
Match: UBC9B_DANRE (SUMO-conjugating enzyme UBC9-B OS=Danio rerio GN=ube2ib PE=1 SV=1)

HSP 1 Score: 87.0409 bits (214), Expect = 9.199e-24
Identity = 41/98 (41.84%), Postives = 56/98 (57.14%), Query Frame = 2 
Query:   89 MSTPAKKRLMRDFKRLQQDPPAGISGAPQDN-----NIMLWNAVIFGPDDTPWDGGTFKLTLQFTEDYPNKPPTVRFVSRMFHPNIYADGSICLDILQ 367
            MS  A  RL ++ K  ++D P G    P  N     N+M W   I G   TPW+GG FKL + F +DYP+ PP  +F   +FHPN+Y  G++CL IL+
Sbjct:    1 MSGIALSRLAQERKAWRKDHPFGFVAVPTKNPDGTMNLMNWECAIPGKKGTPWEGGLFKLRMLFKDDYPSSPPKCKFEPPLFHPNVYPSGTVCLSILE 98

HSP 2 Score: 45.0542 bits (105), Expect = 9.199e-24
Identity = 20/47 (42.55%), Postives = 28/47 (59.57%), Query Frame = 3 
Query:  375 WSPIYDVAAILTSIQSLLCDPNPNSPANSEAARLFSENKREYNRRVR 515
            W P   +  IL  IQ LL +PN   PA +EA  ++ +N+ EY +RVR
Sbjct:  103 WRPAITIKQILLGIQELLNEPNIQDPAQAEAYTIYCQNRVEYEKRVR 149
[Close]
BLAST of EY675113 vs. ExPASy Swiss-Prot
Match: UBC9_SCHPO (SUMO-conjugating enzyme ubc9 OS=Schizosaccharomyces pombe GN=hus5 PE=1 SV=1)

HSP 1 Score: 83.1889 bits (204), Expect = 1.558e-23
Identity = 39/97 (40.21%), Postives = 56/97 (57.73%), Query Frame = 2 
Query:   89 MSTPAKKRLMRDFKRLQQDPPAGISGAPQDNN-----IMLWNAVIFGPDDTPWDGGTFKLTLQFTEDYPNKPPTVRFVSRMFHPNIYADGSICLDIL 364
            MS+  K RL  + K+ ++D P G    P  ++     +M W   I G   T W+GG +KLT+ F E+YP +PP  RF   +FHPN+Y  G++CL IL
Sbjct:    1 MSSLCKTRLQEERKQWRRDHPFGFYAKPCKSSDGGLDLMNWKVGIPGKPKTSWEGGLYKLTMAFPEEYPTRPPKCRFTPPLFHPNVYPSGTVCLSIL 97

HSP 2 Score: 48.1358 bits (113), Expect = 1.558e-23
Identity = 23/47 (48.94%), Postives = 29/47 (61.70%), Query Frame = 3 
Query:  375 WSPIYDVAAILTSIQSLLCDPNPNSPANSEAARLFSENKREYNRRVR 515
            W P   +  IL  IQ LL DPN  SPA +EA  +F ++K EY +RVR
Sbjct:  103 WKPAITIKQILLGIQDLLDDPNIASPAQTEAYTMFKKDKVEYEKRVR 149
[Close]
BLAST of EY675113 vs. ExPASy Swiss-Prot
Match: UBC11_SCHPO (Ubiquitin-conjugating enzyme E2-20 kDa OS=Schizosaccharomyces pombe GN=ubc11 PE=2 SV=1)

HSP 1 Score: 80.1073 bits (196), Expect = 2.017e-23
Identity = 38/89 (42.70%), Postives = 54/89 (60.67%), Query Frame = 2 
Query:  107 KRLMRDFKRLQQDPPAGISGAPQ-DNNIMLWNAVIFGPDDTPWDGGTFKLTLQFTEDYPNKPPTVRFVSRMFHPNIYADGSICLDILQN 370
            KRL  +   L      GIS  P  D+N++ W   I GP DT ++G  FK+++ F  +YP  PPT+ F S M+HPN+   G+ICLDIL++
Sbjct:   31 KRLRSELMSLMMSNTPGISAFPDSDSNLLHWAGTITGPSDTYYEGLKFKISMSFPANYPYSPPTIIFTSPMWHPNVDMSGNICLDILKD 119

HSP 2 Score: 50.8322 bits (120), Expect = 2.017e-23
Identity = 28/58 (48.28%), Postives = 38/58 (65.52%), Query Frame = 3 
Query:  366 KIXWSPIYDVAAILTSIQSLLCDPNPNSPANSEAARLFSENKREYNR----RVREIVE 527
            K  WS +Y+V  IL S+QSLL +PN  SP N++AA L+S++  EY R    R +EI E
Sbjct:  118 KDKWSAVYNVQTILLSLQSLLGEPNNASPLNAQAAELWSKDPIEYKRLLMQRYKEIDE 175
[Close]
BLAST of EY675113 vs. ExPASy Swiss-Prot
Match: UBC11_YEAST (Ubiquitin-conjugating enzyme E2-18 kDa OS=Saccharomyces cerevisiae GN=UBC11 PE=1 SV=1)

HSP 1 Score: 80.8777 bits (198), Expect = 2.028e-23
Identity = 38/88 (43.18%), Postives = 55/88 (62.50%), Query Frame = 2 
Query:  107 KRLMRDFKRLQQDPPAGISGAP-QDNNIMLWNAVIFGPDDTPWDGGTFKLTLQFTEDYPNKPPTVRFVSRMFHPNIYADGSICLDILQ 367
            KRL  +  +L       IS  P  DN++  W   I GP DTP+ G  FK++L+F ++YP  PP ++F+S M+HPN+   G+ICLDIL+
Sbjct:   11 KRLQNELLQLLSSTTESISAFPVDDNDLTYWVGYITGPKDTPYSGLKFKVSLKFPQNYPFHPPMIKFLSPMWHPNVDKSGNICLDILK 98

HSP 2 Score: 50.0618 bits (118), Expect = 2.028e-23
Identity = 24/55 (43.64%), Postives = 34/55 (61.82%), Query Frame = 3 
Query:  366 KIXWSPIYDVAAILTSIQSLLCDPNPNSPANSEAARLFSENKREYNRRVREIVEQ 530
            K  WS +Y+V  IL S+QSLL +PN  SP N+ AA L+  +  EY ++V    E+
Sbjct:   98 KEKWSAVYNVETILLSLQSLLGEPNNRSPLNAVAAELWDADMEEYRKKVLACYEE 152
[Close]
BLAST of EY675113 vs. ExPASy Swiss-Prot
Match: UBE2C_SPISO (Ubiquitin-conjugating enzyme E2 C OS=Spisula solidissima GN=UBE2C PE=1 SV=1)

HSP 1 Score: 84.7297 bits (208), Expect = 2.623e-23
Identity = 40/94 (42.55%), Postives = 57/94 (60.64%), Query Frame = 2 
Query:  107 KRLMRDFKRLQQDPPAGISGAPQDNNIMLWNAVIFGPDDTPWDGGTFKLTLQFTEDYPNKPPTVRFVSRMFHPNIYADGSICLDIL-QNXVESY 385
            KRL ++ + L      GI+  P  +N+  W A + GP DT ++   +KLTL+F  DYP KPP V+F +  +HPN+   G+ICLDIL +N   SY
Sbjct:   33 KRLQQELRTLLMSGDPGITAFPDGDNLFKWVATLDGPKDTVYESLKYKLTLEFPSDYPYKPPVVKFTTPCWHPNVDQSGNICLDILKENWTASY 126

HSP 2 Score: 45.8246 bits (107), Expect = 2.623e-23
Identity = 24/51 (47.06%), Postives = 34/51 (66.67%), Query Frame = 3 
Query:  366 KIXWSPIYDVAAILTSIQSLLCDPNPNSPANSEAARLFSENKREYNRRVRE 518
            K  W+  YDV  IL S+QSLL +PN  SP N++AA ++S N+ EY + + E
Sbjct:  119 KENWTASYDVRTILLSLQSLLGEPNNASPLNAQAADMWS-NQTEYKKVLHE 168
[Close]
BLAST of EY675113 vs. ExPASy Swiss-Prot
Match: UBE2N_DICDI (Probable ubiquitin-conjugating enzyme E2 N OS=Dictyostelium discoideum GN=ube2n PE=3 SV=2)

HSP 1 Score: 87.0409 bits (214), Expect = 2.641e-23
Identity = 39/88 (44.32%), Postives = 58/88 (65.91%), Query Frame = 2 
Query:  107 KRLMRDFKRLQQDPPAGISGAPQDNNIMLWNAVIFGPDDTPWDGGTFKLTLQFTEDYPNKPPTVRFVSRMFHPNIYADGSICLDILQN 370
            KR++++ +RL  +P  GIS  P  +N   +   I GP D+P++GG F L L  T+DYP   P VRF+++++HPNI   G ICLDIL++
Sbjct:    6 KRIIKETQRLMTEPAPGISATPATDNFRYFKVAISGPLDSPFEGGVFNLELFLTDDYPMSAPKVRFLTKIYHPNIDKLGRICLDILKD 93

HSP 2 Score: 43.5134 bits (101), Expect = 2.641e-23
Identity = 20/51 (39.22%), Postives = 29/51 (56.86%), Query Frame = 3 
Query:  366 KIXWSPIYDVAAILTSIQSLLCDPNPNSPANSEAARLFSENKREYNRRVRE 518
            K  WSP   +  +L SIQ+LL  PNP+ P  ++ A  +  N++E   R RE
Sbjct:   92 KDKWSPALQIRTVLLSIQALLSAPNPDDPLANDVAEHWKTNEKEAMARARE 142
[Close]
BLAST of EY675113 vs. ExPASy Swiss-Prot
Match: UBC35_ARATH (Ubiquitin carrier protein E2 35 OS=Arabidopsis thaliana GN=UBC35 PE=1 SV=1)

HSP 1 Score: 89.3521 bits (220), Expect = 9.857e-23
Identity = 38/88 (43.18%), Postives = 62/88 (70.45%), Query Frame = 2 
Query:  107 KRLMRDFKRLQQDPPAGISGAPQDNNIMLWNAVIFGPDDTPWDGGTFKLTLQFTEDYPNKPPTVRFVSRMFHPNIYADGSICLDILQN 370
            +R++++ +RL  +P  GIS +P ++N+  +N +I GP  +P++GG FKL L   E+YP   P VRF+++++HPNI   G ICLDIL++
Sbjct:    8 RRIIKETQRLLSEPAPGISASPSEDNMRYFNVMILGPTQSPYEGGVFKLELFLPEEYPMAAPKVRFLTKIYHPNIDKLGRICLDILKD 95

HSP 2 Score: 39.2762 bits (90), Expect = 9.857e-23
Identity = 17/44 (38.64%), Postives = 25/44 (56.82%), Query Frame = 3 
Query:  366 KIXWSPIYDVAAILTSIQSLLCDPNPNSPANSEAARLFSENKRE 497
            K  WSP   +  +L SIQ+LL  PNP+ P +   A+ +  N+ E
Sbjct:   94 KDKWSPALQIRTVLLSIQALLSAPNPDDPLSENIAKHWKSNEAE 137
[Close]
BLAST of EY675113 vs. ExPASy Swiss-Prot
Match: UBC36_ARATH (Ubiquitin carrier protein E2 36 OS=Arabidopsis thaliana GN=UBC36 PE=1 SV=1)

HSP 1 Score: 88.9669 bits (219), Expect = 9.857e-23
Identity = 38/88 (43.18%), Postives = 61/88 (69.32%), Query Frame = 2 
Query:  107 KRLMRDFKRLQQDPPAGISGAPQDNNIMLWNAVIFGPDDTPWDGGTFKLTLQFTEDYPNKPPTVRFVSRMFHPNIYADGSICLDILQN 370
            +R++++ +RL  +P  GIS +P + N+  +N +I GP  +P++GG FKL L   E+YP   P VRF+++++HPNI   G ICLDIL++
Sbjct:    8 RRIIKETQRLLSEPAPGISASPSEENMRYFNVMILGPTQSPYEGGVFKLELFLPEEYPMAAPKVRFLTKIYHPNIDKLGRICLDILKD 95

HSP 2 Score: 39.6614 bits (91), Expect = 9.857e-23
Identity = 18/51 (35.29%), Postives = 27/51 (52.94%), Query Frame = 3 
Query:  366 KIXWSPIYDVAAILTSIQSLLCDPNPNSPANSEAARLFSENKREYNRRVRE 518
            K  WSP   +  +L SIQ+LL  PNP+ P +   A+ +  N+ E     +E
Sbjct:   94 KDKWSPALQIRTVLLSIQALLSAPNPDDPLSENIAKHWKSNEAEAVETAKE 144
[Close]
BLAST of EY675113 vs. ExPASy Swiss-Prot
Match: UBC9_PAGMA (SUMO-conjugating enzyme UBC9 OS=Pagrus major GN=ube2i PE=2 SV=1)

HSP 1 Score: 83.1889 bits (204), Expect = 1.281e-22
Identity = 40/98 (40.82%), Postives = 56/98 (57.14%), Query Frame = 2 
Query:   89 MSTPAKKRLMRDFKRLQQDPPAGISGAPQDN-----NIMLWNAVIFGPDDTPWDGGTFKLTLQFTEDYPNKPPTVRFVSRMFHPNIYADGSICLDILQ 367
            MS  A  RL ++ K  ++D P G+   P  N     N+M     I G   TPW+GG FKL + F +DYP+ PP  +F   +FHPN+Y  G++CL IL+
Sbjct:    1 MSGIALSRLAQERKAWRKDHPLGLVAVPTKNPDGTMNLMNGECAIPGKKGTPWEGGLFKLRMLFKDDYPSSPPKCKFEPPLFHPNVYPSGTVCLSILE 98

HSP 2 Score: 45.0542 bits (105), Expect = 1.281e-22
Identity = 20/47 (42.55%), Postives = 28/47 (59.57%), Query Frame = 3 
Query:  375 WSPIYDVAAILTSIQSLLCDPNPNSPANSEAARLFSENKREYNRRVR 515
            W P   +  IL  IQ LL +PN   PA +EA  +F +++ EY +RVR
Sbjct:  103 WRPAITIKQILLGIQELLNEPNIQDPAQAEAYTIFCQDRTEYEKRVR 149
[Close]
BLAST of EY675113 vs. ExPASy Swiss-Prot
Match: UBC13_YEAST (Ubiquitin-conjugating enzyme E2 13 OS=Saccharomyces cerevisiae GN=UBC13 PE=1 SV=1)

HSP 1 Score: 84.3445 bits (207), Expect = 1.669e-22
Identity = 35/87 (40.23%), Postives = 59/87 (67.82%), Query Frame = 2 
Query:  107 KRLMRDFKRLQQDPPAGISGAPQDNNIMLWNAVIFGPDDTPWDGGTFKLTLQFTEDYPNKPPTVRFVSRMFHPNIYADGSICLDILQ 367
            KR++++ ++L  DP  GI+  P D+N+  +   I GP+ +P++ G F+L L   +DYP + P VRF+++++HPNI   G ICLD+L+
Sbjct:    6 KRIIKETEKLVSDPVPGITAEPHDDNLRYFQVTIEGPEQSPYEDGIFELELYLPDDYPMEAPKVRFLTKIYHPNIDRLGRICLDVLK 92

HSP 2 Score: 43.5134 bits (101), Expect = 1.669e-22
Identity = 19/51 (37.25%), Postives = 29/51 (56.86%), Query Frame = 3 
Query:  366 KIXWSPIYDVAAILTSIQSLLCDPNPNSPANSEAARLFSENKREYNRRVRE 518
            K  WSP   +  +L SIQ+LL  PNPN P  ++ A  + +N++    + RE
Sbjct:   92 KTNWSPALQIRTVLLSIQALLASPNPNDPLANDVAEDWIKNEQGAKAKARE 142
The following BLAST results are available for this feature:
BLAST of EY675113 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 228
Match NameE-valueIdentityDescription
UBC9B_DANRE9.199e-2441.84SUMO-conjugating enzyme UBC9-B OS=Danio rerio GN=u... [more]
UBC9_SCHPO1.558e-2340.21SUMO-conjugating enzyme ubc9 OS=Schizosaccharomyce... [more]
UBC11_SCHPO2.017e-2342.70Ubiquitin-conjugating enzyme E2-20 kDa OS=Schizosa... [more]
UBC11_YEAST2.028e-2343.18Ubiquitin-conjugating enzyme E2-18 kDa OS=Saccharo... [more]
UBE2C_SPISO2.623e-2342.55Ubiquitin-conjugating enzyme E2 C OS=Spisula solid... [more]
UBE2N_DICDI2.641e-2344.32Probable ubiquitin-conjugating enzyme E2 N OS=Dict... [more]
UBC35_ARATH9.857e-2343.18Ubiquitin carrier protein E2 35 OS=Arabidopsis tha... [more]
UBC36_ARATH9.857e-2343.18Ubiquitin carrier protein E2 36 OS=Arabidopsis tha... [more]
UBC9_PAGMA1.281e-2240.82SUMO-conjugating enzyme UBC9 OS=Pagrus major GN=ub... [more]
UBC13_YEAST1.669e-2240.23Ubiquitin-conjugating enzyme E2 13 OS=Saccharomyce... [more]

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Properties
Property NameValue
Genbank descriptionCS00-C1-401-006-C04-CT.F Sweet orange leaf, infected with CiLV Citrus sinensis cDNA, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>EY675113 ID=EY675113; Name=EY675113; organism=Citrus sinensis; type=EST; length=988bp
TTCTTTCTCTAGAAGCTTCTCCTCTCATTCTCCCCCCTTACCCCCTCTGC
AAAAGCCGCTTCTCAGTTTTGCTCTCTAAAACGCCACTATGTCAACACCG
GCGAAGAAGCGGCTGATGCGGGACTTCAAGCGCCTCCAGCAAGATCCACC
CGCCGGAATCAGCGGGGCCCCTCAAGACAACAACATCATGTTATGGAACG
CCGTTATTTTTGGGCCTGATGATACTCCCTGGGACGGAGGTACGTTCAAG
TTGACTCTTCAGTTTACAGAGGATTATCCGAATAAACCTCCAACAGTTCG
GTTCGTTTCACGGATGTTTCATCCAAATATCTATGCGGATGGAAGTATCT
GCCTGGATATATTACAAAATCANGTGGAGTCCTATATATGATGTTGCAGC
TATACTGACCTCCATCCAGTCGCTGCTTTGTGATCCAAATCCAAACTCCC
CTGCAAATTCTGAAGCAGCTCGGCTGTTTAGTGAGAATAAACGAGAGTAC
AACCGAAGAGTGCGTGAAATAGTGGAGCAAAGCTGGACAGCCGACTAAAA
GTCCCATGAAAGCACCGGCCGGTTATGCTGGAGAAATAAAAGATGAAAAC
TGGGGCTGGTGGGGTTGGTTTGGTCAAAATAATGGAACCCGTATTCAATC
GGATTTCAGGTGCATTGTTAATTAAAAAAATCCCCTTCGAATCTTTCCTC
CCCAAAAACCCTGGGTGGAACCCTTGGTTATTTTTCCAACATTTCCCTCT
TCCTTTTCCAAAACTTTCCGGGTAAATGTAATTTTATTGGGGACATTTTG
GTAAGGGGGGTTTTTTGGGGGGCGGGAAAACCCTGTGGATGTTGTTCTTT
AAAAAAACCCCCCCAAAAAAGAAACCCCTTTTTTTTTTTGGGAAATTTTT
TTTATGGGGGGATATTTTTTTGGGGGGATTTGTGGTGGGCGGGGGGGCGC
CCAAAAAAAAATAAAAAAAAAAAAAGGAAACAAAAACA
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