EY674782

Overview
NameEY674782
Unique NameEY674782
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length1038
Libraries
Library NameType
Sweet orange leaf, infected with CiLVcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of EY674782 vs. ExPASy Swiss-Prot
Match: KS6B1_RAT (Ribosomal protein S6 kinase beta-1 OS=Rattus norvegicus GN=Rps6kb1 PE=1 SV=1)

HSP 1 Score: 94.3597 bits (233), Expect = 1.200e-18
Identity = 58/136 (42.65%), Postives = 81/136 (59.56%), Query Frame = 1 
Query:    7 VRKRGVLP*GVSPDVLMLDKTLHLQLVDFRFGKGL--SGNRTFTICGIPDYLAPEIVQGKGHGLAADWWALGVLIYFMLQGEMPF-GSWRESEIDIVAKIAKGQLSLPQNLSHEAVDLLTKLLVVDENTRLGSQGP 405
            + ++G++   + P+ +ML+   H++L DF   K     G  T T CG  +Y+APEI+   GH  A DWW+LG L+Y ML G  PF G  R+  ID   KI K +L+LP  L+ EA DLL KLL  +  +RLG+ GP
Sbjct:  209 LHQKGIIYRDLKPENIMLNHQGHVKLTDFGLCKESIHDGTVTHTFCGTIEYMAPEILMRSGHNRAVDWWSLGALMYDMLTGAPPFTGENRKKTID---KILKCKLNLPPYLTQEARDLLKKLLKRNAASRLGA-GP 340
[Close]
BLAST of EY674782 vs. ExPASy Swiss-Prot
Match: KS6B1_RABIT (Ribosomal protein S6 kinase beta-1 OS=Oryctolagus cuniculus GN=RPS6KB1 PE=2 SV=1)

HSP 1 Score: 94.3597 bits (233), Expect = 1.200e-18
Identity = 58/136 (42.65%), Postives = 81/136 (59.56%), Query Frame = 1 
Query:    7 VRKRGVLP*GVSPDVLMLDKTLHLQLVDFRFGKGL--SGNRTFTICGIPDYLAPEIVQGKGHGLAADWWALGVLIYFMLQGEMPF-GSWRESEIDIVAKIAKGQLSLPQNLSHEAVDLLTKLLVVDENTRLGSQGP 405
            + ++G++   + P+ +ML+   H++L DF   K     G  T T CG  +Y+APEI+   GH  A DWW+LG L+Y ML G  PF G  R+  ID   KI K +L+LP  L+ EA DLL KLL  +  +RLG+ GP
Sbjct:  209 LHQKGIIYRDLKPENIMLNHQGHVKLTDFGLCKESIHDGTVTHTFCGTIEYMAPEILMRSGHNRAVDWWSLGALMYDMLTGAPPFTGENRKKTID---KILKCKLNLPPYLTQEARDLLKKLLKRNAASRLGA-GP 340
[Close]
BLAST of EY674782 vs. ExPASy Swiss-Prot
Match: KS6B1_MOUSE (Ribosomal protein S6 kinase beta-1 OS=Mus musculus GN=Rps6kb1 PE=2 SV=1)

HSP 1 Score: 94.3597 bits (233), Expect = 1.200e-18
Identity = 58/136 (42.65%), Postives = 81/136 (59.56%), Query Frame = 1 
Query:    7 VRKRGVLP*GVSPDVLMLDKTLHLQLVDFRFGKGL--SGNRTFTICGIPDYLAPEIVQGKGHGLAADWWALGVLIYFMLQGEMPF-GSWRESEIDIVAKIAKGQLSLPQNLSHEAVDLLTKLLVVDENTRLGSQGP 405
            + ++G++   + P+ +ML+   H++L DF   K     G  T T CG  +Y+APEI+   GH  A DWW+LG L+Y ML G  PF G  R+  ID   KI K +L+LP  L+ EA DLL KLL  +  +RLG+ GP
Sbjct:  209 LHQKGIIYRDLKPENIMLNHQGHVKLTDFGLCKESIHDGTVTHTFCGTIEYMAPEILMRSGHNRAVDWWSLGALMYDMLTGAPPFTGENRKKTID---KILKCKLNLPPYLTQEARDLLKKLLKRNAASRLGA-GP 340
[Close]
BLAST of EY674782 vs. ExPASy Swiss-Prot
Match: KS6B1_HUMAN (Ribosomal protein S6 kinase beta-1 OS=Homo sapiens GN=RPS6KB1 PE=1 SV=2)

HSP 1 Score: 94.3597 bits (233), Expect = 1.200e-18
Identity = 58/136 (42.65%), Postives = 81/136 (59.56%), Query Frame = 1 
Query:    7 VRKRGVLP*GVSPDVLMLDKTLHLQLVDFRFGKGL--SGNRTFTICGIPDYLAPEIVQGKGHGLAADWWALGVLIYFMLQGEMPF-GSWRESEIDIVAKIAKGQLSLPQNLSHEAVDLLTKLLVVDENTRLGSQGP 405
            + ++G++   + P+ +ML+   H++L DF   K     G  T T CG  +Y+APEI+   GH  A DWW+LG L+Y ML G  PF G  R+  ID   KI K +L+LP  L+ EA DLL KLL  +  +RLG+ GP
Sbjct:  209 LHQKGIIYRDLKPENIMLNHQGHVKLTDFGLCKESIHDGTVTHTFCGTIEYMAPEILMRSGHNRAVDWWSLGALMYDMLTGAPPFTGENRKKTID---KILKCKLNLPPYLTQEARDLLKKLLKRNAASRLGA-GP 340
[Close]
BLAST of EY674782 vs. ExPASy Swiss-Prot
Match: KS6B1_BOVIN (Ribosomal protein S6 kinase beta-1 OS=Bos taurus GN=RPS6KB1 PE=2 SV=1)

HSP 1 Score: 94.3597 bits (233), Expect = 1.200e-18
Identity = 58/136 (42.65%), Postives = 81/136 (59.56%), Query Frame = 1 
Query:    7 VRKRGVLP*GVSPDVLMLDKTLHLQLVDFRFGKGL--SGNRTFTICGIPDYLAPEIVQGKGHGLAADWWALGVLIYFMLQGEMPF-GSWRESEIDIVAKIAKGQLSLPQNLSHEAVDLLTKLLVVDENTRLGSQGP 405
            + ++G++   + P+ +ML+   H++L DF   K     G  T T CG  +Y+APEI+   GH  A DWW+LG L+Y ML G  PF G  R+  ID   KI K +L+LP  L+ EA DLL KLL  +  +RLG+ GP
Sbjct:  209 LHQKGIIYRDLKPENIMLNHQGHVKLTDFGLCKESIHDGTVTHTFCGTIEYMAPEILMRSGHNRAVDWWSLGALMYDMLTGAPPFTGENRKKTID---KILKCKLNLPPYLTQEARDLLKKLLKRNAASRLGA-GP 340
[Close]
BLAST of EY674782 vs. ExPASy Swiss-Prot
Match: KPCE_RABIT (Protein kinase C epsilon type OS=Oryctolagus cuniculus GN=PRKCE PE=2 SV=1)

HSP 1 Score: 89.7373 bits (221), Expect = 1.306e-18
Identity = 54/146 (36.99%), Postives = 80/146 (54.79%), Query Frame = 1 
Query:    7 VRKRGVLP*GVSPDVLMLDKTLHLQLVDFRFGKG--LSGNRTFTICGIPDYLAPEIVQGKGHGLAADWWALGVLIYFMLQGEMPFGSWRESEIDIVAKIAKGQLSLPQNLSHEAVDLLTKLLVVDENTRLG----SQGPTSVKNSP 426
            + + GV+   +  D ++LD   H +L DF   K   L+G  T T CG PDY+APEI+Q   +G + DWWALGVL+Y M+ G+ PF +  ++E D+   I    +  P  LS EAV +L   +  + + RLG      G  ++K  P
Sbjct:  522 LHQHGVIYRDLKLDNILLDAEGHCKLADFGMCKEGILNGVTTTTFCGTPDYIAPEILQELEYGPSVDWWALGVLMYEMMAGQPPFEA--DNEDDLFESILHDDVLYPVWLSKEAVSILKAFMTKNPHKRLGCVAAQNGEDAIKQHP 665

HSP 2 Score: 25.0238 bits (53), Expect = 1.306e-18
Identity = 7/11 (63.64%), Postives = 10/11 (90.91%), Query Frame = 2 
Query:  416 KTHPWFKDVDW 448
            K HP+FK++DW
Sbjct:  662 KQHPFFKEIDW 672
[Close]
BLAST of EY674782 vs. ExPASy Swiss-Prot
Match: KPCE_HUMAN (Protein kinase C epsilon type OS=Homo sapiens GN=PRKCE PE=1 SV=1)

HSP 1 Score: 89.7373 bits (221), Expect = 1.306e-18
Identity = 54/146 (36.99%), Postives = 80/146 (54.79%), Query Frame = 1 
Query:    7 VRKRGVLP*GVSPDVLMLDKTLHLQLVDFRFGKG--LSGNRTFTICGIPDYLAPEIVQGKGHGLAADWWALGVLIYFMLQGEMPFGSWRESEIDIVAKIAKGQLSLPQNLSHEAVDLLTKLLVVDENTRLG----SQGPTSVKNSP 426
            + + GV+   +  D ++LD   H +L DF   K   L+G  T T CG PDY+APEI+Q   +G + DWWALGVL+Y M+ G+ PF +  ++E D+   I    +  P  LS EAV +L   +  + + RLG      G  ++K  P
Sbjct:  523 LHQHGVIYRDLKLDNILLDAEGHCKLADFGMCKEGILNGVTTTTFCGTPDYIAPEILQELEYGPSVDWWALGVLMYEMMAGQPPFEA--DNEDDLFESILHDDVLYPVWLSKEAVSILKAFMTKNPHKRLGCVASQNGEDAIKQHP 666

HSP 2 Score: 25.0238 bits (53), Expect = 1.306e-18
Identity = 7/11 (63.64%), Postives = 10/11 (90.91%), Query Frame = 2 
Query:  416 KTHPWFKDVDW 448
            K HP+FK++DW
Sbjct:  663 KQHPFFKEIDW 673
[Close]
BLAST of EY674782 vs. ExPASy Swiss-Prot
Match: KPC1B_CAEEL (Protein kinase C-like 1B OS=Caenorhabditis elegans GN=pkc-1 PE=2 SV=2)

HSP 1 Score: 87.4261 bits (215), Expect = 1.307e-18
Identity = 52/124 (41.94%), Postives = 74/124 (59.68%), Query Frame = 1 
Query:   46 DVLMLDKTLHLQLVDFRFGK-GLS-GNRTFTICGIPDYLAPEIVQGKGHGLAADWWALGVLIYFMLQGEMPFGSWRESEIDIVAKIAKGQLSLPQNLSHEAVDLLTKLLVVDENTRLG---SQG 402
            D ++LD   H +L DF   K G++  N T T CG PDY+APEI+Q   +G++ DWWALGVL+Y M+ G+ PF +  ++E D+   I    +  P  LS EAV++L   +  +   RLG   SQG
Sbjct:  506 DNILLDAEGHCRLADFGMCKEGINKDNLTSTFCGTPDYIAPEILQEMEYGVSVDWWALGVLMYEMMAGQPPFEA--DNEDDLFEAILNDDVLYPVWLSKEAVNILKAFMTKNAGKRLGCVVSQG 627

HSP 2 Score: 27.335 bits (59), Expect = 1.307e-18
Identity = 12/50 (24.00%), Postives = 25/50 (50.00%), Query Frame = 2 
Query:  416 KTHPWFKDVDWKGI-AESTSPCSHMKKMSPHKSNTWNRSFLEGFPLVFSS 562
            + HP+F+++DW  + +    P    K  S   +N ++  F +  P++  S
Sbjct:  633 RAHPFFREIDWDALESRQVKPPFKPKIKSKRDANNFDSDFTKEEPVLTPS 682
[Close]
BLAST of EY674782 vs. ExPASy Swiss-Prot
Match: KPCE_RAT (Protein kinase C epsilon type OS=Rattus norvegicus GN=Prkce PE=1 SV=1)

HSP 1 Score: 89.3521 bits (220), Expect = 1.698e-18
Identity = 54/146 (36.99%), Postives = 80/146 (54.79%), Query Frame = 1 
Query:    7 VRKRGVLP*GVSPDVLMLDKTLHLQLVDFRFGKG--LSGNRTFTICGIPDYLAPEIVQGKGHGLAADWWALGVLIYFMLQGEMPFGSWRESEIDIVAKIAKGQLSLPQNLSHEAVDLLTKLLVVDENTRLG----SQGPTSVKNSP 426
            + + GV+   +  D ++LD   H +L DF   K   L+G  T T CG PDY+APEI+Q   +G + DWWALGVL+Y M+ G+ PF +  ++E D+   I    +  P  LS EAV +L   +  + + RLG      G  ++K  P
Sbjct:  523 LHQHGVIYRDLKLDNILLDAEGHSKLADFGMCKEGILNGVTTTTFCGTPDYIAPEILQELEYGPSVDWWALGVLMYEMMAGQPPFEA--DNEDDLFESILHDDVLYPVWLSKEAVSILKAFMTKNPHKRLGCVAAQNGEDAIKQHP 666

HSP 2 Score: 25.0238 bits (53), Expect = 1.698e-18
Identity = 7/11 (63.64%), Postives = 10/11 (90.91%), Query Frame = 2 
Query:  416 KTHPWFKDVDW 448
            K HP+FK++DW
Sbjct:  663 KQHPFFKEIDW 673
[Close]
BLAST of EY674782 vs. ExPASy Swiss-Prot
Match: PKD1_DICDI (Developmentally-regulated protein kinase 1 OS=Dictyostelium discoideum GN=pkaD PE=2 SV=2)

HSP 1 Score: 91.6633 bits (226), Expect = 1.699e-18
Identity = 50/131 (38.17%), Postives = 81/131 (61.83%), Query Frame = 1 
Query:   37 VSPDVLMLDKTLHLQLVDFRFGKG-LSGNRTFTICGIPDYLAPEIVQGK-GHGLAADWWALGVLIYFMLQGEMPFGSWRESEIDIVAKIAKGQLSLPQN-LSHEAVDLLTKLLVVDENTRLGSQGPTSVKN 420
            + P+ +++D+  H++L DF F K  + G  T ++CG  DY+APEI+    GHG   DWWALGV++Y ++ G++PF + +ES   ++ + A  QL    + LS E  D + +LL VD + RLG+    S++N
Sbjct:  458 LKPENILIDEKGHIKLTDFGFSKKTVGGKNTSSVCGTFDYMAPEILNSSNGHGKPVDWWALGVVVYELVTGKLPFSNSKES---LLNRKADFQLIFQNSYLSDEIKDFIFQLLSVDPSKRLGTFDSCSIRN 585

HSP 2 Score: 22.7126 bits (47), Expect = 1.699e-18
Identity = 5/11 (45.45%), Postives = 8/11 (72.73%), Query Frame = 2 
Query:  416 KTHPWFKDVDW 448
            + H WF D++W
Sbjct:  584 RNHKWFSDINW 594
The following BLAST results are available for this feature:
BLAST of EY674782 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 375
Match NameE-valueIdentityDescription
KS6B1_RAT1.200e-1842.65Ribosomal protein S6 kinase beta-1 OS=Rattus norve... [more]
KS6B1_RABIT1.200e-1842.65Ribosomal protein S6 kinase beta-1 OS=Oryctolagus ... [more]
KS6B1_MOUSE1.200e-1842.65Ribosomal protein S6 kinase beta-1 OS=Mus musculus... [more]
KS6B1_HUMAN1.200e-1842.65Ribosomal protein S6 kinase beta-1 OS=Homo sapiens... [more]
KS6B1_BOVIN1.200e-1842.65Ribosomal protein S6 kinase beta-1 OS=Bos taurus G... [more]
KPCE_RABIT1.306e-1836.99Protein kinase C epsilon type OS=Oryctolagus cunic... [more]
KPCE_HUMAN1.306e-1836.99Protein kinase C epsilon type OS=Homo sapiens GN=P... [more]
KPC1B_CAEEL1.307e-1841.94Protein kinase C-like 1B OS=Caenorhabditis elegans... [more]
KPCE_RAT1.698e-1836.99Protein kinase C epsilon type OS=Rattus norvegicus... [more]
PKD1_DICDI1.699e-1838.17Developmentally-regulated protein kinase 1 OS=Dict... [more]

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Properties
Property NameValue
Genbank descriptionCS00-C1-401-001-D03-CT.F Sweet orange leaf, infected with CiLV Citrus sinensis cDNA, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>EY674782 ID=EY674782; Name=EY674782; organism=Citrus sinensis; type=EST; length=1038bp
CCACGCGTCCGCAAGAGAGGTGTTCTCCCCTGAGGTGTGTCTCCTGATGT
TCTAATGTTGGACAAAACCCTACATTTACAGTTAGTAGACTTTCGATTCG
GGAAAGGCTTATCTGGCAACAGAACATTCACAATTTGTGGAATCCCAGAC
TATTTAGCTCCAGAGATAGTTCAGGGAAAAGGCCATGGTCTCGCTGCTGA
CTGGTGGGCATTGGGAGTCCTAATTTATTTCATGTTACAAGGTGAAATGC
CATTTGGATCTTGGAGAGAGAGTGAAATAGATATTGTTGCAAAGATTGCA
AAAGGGCAGCTAAGTCTTCCACAGAATTTAAGCCATGAAGCTGTTGATCT
CCTTACAAAGTTGCTCGTAGTTGATGAAAATACAAGACTTGGTAGCCAAG
GTCCCACTTCTGTCAAAAACTCACCCATGGTTCAAAGATGTTGATTGGAA
AGGGATTGCTGAATCTACTTCTCCTTGTTCCCACATGAAAAAAATGTCTC
CGCATAAGTCAAACACCTGGAATAGGTCATTTTTGGAGGGATTTCCCCTG
GTTTTTTCAAGCTTCCTCCGCCCTTCGAAATGTTGAAAGAACTCAAATGG
TTCCTGAAAGGGGTTTGATTAACTGGGAAGGAGTTTAAAATTGGGTATCC
TTTATTTTTTGGGTTTCTGGTTTTCCCCTATTTAATTCCTTGGCCAAAAA
ATTTTCTTTTCCCTTGGGTTTTCCTTCCCCCTGCCTGGGTCCCTTGGGCC
TTAATTTTGGAAAGTTTTTGTAAAAAAAACCCCCACCTTTTTTCCAAAAC
TTTCAAAATATGGGGGTAAAACACAAAATTATAAATAAAAAACAAAAAAA
TTATAAAATTTTGGCGGGGTTGTCGCCCCCCCCACGGGGCCCCCATTAAA
TTTTTTGGCCCCACAAAAAAAACCCCCAATTATAAATTTATTTTTGGTTT
TTTTTGTGGTGGGCCCCCCACAATATGTTATAAGAGAATAGAGGAAGACG
CGGAAAAGGAGGGGGGGGGGTGTGGTGTGGGGTGGTGT
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