EY703166

Overview
NameEY703166
Unique NameEY703166
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length913
Libraries
Library NameType
Sweet orange fruit, development stadium (2 of 6)cdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of EY703166 vs. ExPASy Swiss-Prot
Match: CB4A_SOLLC (Chlorophyll a-b binding protein CP24 10A, chloroplastic OS=Solanum lycopersicum GN=CAP10A PE=3 SV=1)

HSP 1 Score: 76.6406 bits (187), Expect = 3.785e-16
Identity = 57/145 (39.31%), Postives = 75/145 (51.72%), Query Frame = 3 
Query:  192 PPYLDGSAPGDFGFDPLRLGEVPENLERYKESELIHCRWAMLAVPGILVPEALGLGNWVQAQEWAAVPGGQATYLGNPVPWGTLPTILVIEFLSIAFVEHQRSME-KDPEKKK----------------------YPGGA-FDPL 554
            P +LDGS PGD+GFDPL LG+ P  L+ Y+E+ELIH RWAM AV GI V +A     W +A    A PG  A     P  +GTL   L  + + + +VE +R ++  DP+ +                       YPGG  FDPL
Sbjct:   68 PEWLDGSLPGDYGFDPLGLGKDPAFLKWYREAELIHGRWAMAAVLGIFVGQAWSGIPWFEA---GADPGAIA-----PFSFGTL---LGTQLILMGWVESKRWVDFFDPDSQSVEWATPWSKTAENFANFTGEQGYPGGKFFDPL 201

HSP 2 Score: 29.6462 bits (65), Expect = 3.785e-16
Identity = 14/26 (53.85%), Postives = 18/26 (69.23%), Query Frame = 2 
Query:  566 DPQKFEEYKVKEIKNGRLALLAFVGF 643
            D +K E  KV EIK+ RLA+LA + F
Sbjct:  215 DTEKLERLKVAEIKHARLAMLAMLIF 240
[Close]
BLAST of EY703166 vs. ExPASy Swiss-Prot
Match: CB2_MALDO (Chlorophyll a-b binding protein AB10, chloroplastic OS=Malus domestica PE=3 SV=1)

HSP 1 Score: 60.077 bits (144), Expect = 8.299e-16
Identity = 59/188 (31.38%), Postives = 81/188 (43.09%), Query Frame = 3 
Query:   36 NTLMSCGIATTAFPSVLSSSKSKFFTAVSLPAAANATSKYA--MSADWMPGEPRPPYLDGSAPGDFGFDPLRLGEVPENLERYKESELIHCRWAMLAVPGILVPEAL-----GLGN--WVQAQEWAAVPGGQATYLGNP--VPWGTLPTILVIEFLSIAFVEHQRSME---KDPEKKKYPGGAFDPLG 557
            N + S  I T  F  +  ++KSK  ++       +   KY    S +W      P YL G  PGD+G+D   L   PE   + +E E+IH R AM A  G + PE L     G G   W +A       GG   YLGNP  V   ++  I   + + +  VE  R       +     YP G+FD LG
Sbjct:   27 NPISSTLIVTAGFTMLKHAAKSKVSSST-----CDRRVKYLGPFSGEW------PSYLTGEFPGDYGWDTAGLSAYPETFAKNRELEVIHSRCAMSAALGCIFPELLSVMGQGFGEAVWFKAGAQIFSEGG-LDYLGNPSLVHAQSILAIWTTKVILMGAVEGYRIARGPLGEVTDPLYP-GSFDSLG 201

HSP 2 Score: 45.0542 bits (105), Expect = 8.299e-16
Identity = 21/37 (56.76%), Postives = 26/37 (70.27%), Query Frame = 2 
Query:  542 FRPLGLLQDPQKFEEYKVKEIKNGRLALLAFVGFCVQ 652
            F  LGL +D + F E KVKE+KNGRLA+ +  GF VQ
Sbjct:  197 FDSLGLAEDTEAFAELKVKELKNGRLAMFSMFGFFVQ 233
[Close]
BLAST of EY703166 vs. ExPASy Swiss-Prot
Match: CB21_SILPR (Chlorophyll a-b binding protein, chloroplastic (Fragment) OS=Silene pratensis PE=2 SV=2)

HSP 1 Score: 76.2554 bits (186), Expect = 3.285e-14
Identity = 52/137 (37.96%), Postives = 66/137 (48.18%), Query Frame = 3 
Query:  183 EPRPPYLDGSAPGDFGFDPLRLGEVPENLERYKESELIHCRWAMLAVPGILVPEAL-------GLGNWVQAQEWAAVPGGQATYLGNP--VPWGTLPTILVIEFLSIAFVEHQR---SMEKDPEKKKYPGGAFDPLG 557
            E  P YL G  PGD+G+D   L   PE   + +E E+IHCRWAML   G + PE L       G   W +A       GG   YLGNP  V   ++  I   + + +  VE  R       +   + YPGGAFDPLG
Sbjct:   62 EQTPSYLTGEFPGDYGWDTAGLSADPETFAKNRELEVIHCRWAMLGALGCVFPELLAKNGVKFGEAVWFKAGSQIFQEGG-LDYLGNPNLVHAQSILAIWACQVVLMGAVEGYRVGGGPLGEGLDQLYPGGAFDPLG 197

HSP 2 Score: 23.483 bits (49), Expect = 3.285e-14
Identity = 9/17 (52.94%), Postives = 11/17 (64.71%), Query Frame = 2 
Query:  530 PRWCFRPLGLLQDPQKF 580
            P   F PLGL +DP+ F
Sbjct:  189 PGGAFDPLGLAEDPEAF 205
[Close]
BLAST of EY703166 vs. ExPASy Swiss-Prot
Match: CB4B_SOLLC (Chlorophyll a-b binding protein CP24 10B, chloroplastic OS=Solanum lycopersicum GN=CAP10B PE=3 SV=1)

HSP 1 Score: 77.0258 bits (188), Expect = 1.640e-13
Identity = 59/169 (34.91%), Postives = 82/169 (48.52%), Query Frame = 3 
Query:   57 IATTAFPSVLSSSKSKFFTA---------VSLPAAANATSKYAMSA-------DWMPGEPR------PPYLDGSAPGDFGFDPLRLGEVPENLERYKESELIHCRWAMLAVPGILVPEALGLGNWVQAQEWAAVPGGQATYLGNPVPWGTLPTILVIEFLSIAFVEHQR 497
            + TT+  +VL+   S F T            + A      ++ ++A        W+P          P +LDGS PGDFGFDPL LG+ P  L+ Y+E+ELIH RWAM AV GI V +A     W +A    A PG  A     P  +G+L   L  + L + +VE +R
Sbjct:    1 MTTTSATAVLNGLSSSFLTGGKKTQALLGAHVTARVTTPKRFVVAAAAVAPKKSWIPAVKSGGNLVDPEWLDGSLPGDFGFDPLGLGKDPAFLKWYREAELIHGRWAMAAVLGIFVGQAWSGIPWFEA---GADPGAIA-----PFSFGSL---LGTQLLLMGWVESKR 158
[Close]
BLAST of EY703166 vs. ExPASy Swiss-Prot
Match: CB21_PINTH (Chlorophyll a-b binding protein type I, chloroplastic OS=Pinus thunbergii PE=2 SV=1)

HSP 1 Score: 69.707 bits (169), Expect = 2.638e-13
Identity = 49/140 (35.00%), Postives = 64/140 (45.71%), Query Frame = 3 
Query:  183 EPRPPYLDGSAPGDFGFDPLRLGEVPENLERYKESELIHCRWAMLAVPGILVPEAL-------GLGNWVQAQEWAAVPGGQATYLGNP--VPWGTLPTILVIEFLSIAFVEHQR------SMEKDPEKKKYPGGAFDPLG 557
            E  P YL G  PGD+G+D   +   PE   + +E E+IHCRWAML   G + PE L       G   W +A       GG   Y GNP  +   ++  I   + + +  +E  R          DP     PGGAFDPLG
Sbjct:   64 EGTPSYLTGEFPGDYGWDTAAVSADPETFAKNRELEVIHCRWAMLGALGCVFPELLAKNGLKFGEAVWFKAGAQIFSEGG-LDYAGNPNLIHAQSILAIWACQVVLMGLIEGYRVGGGTLGEGLDP---LLPGGAFDPLG 199

HSP 2 Score: 26.9498 bits (58), Expect = 2.638e-13
Identity = 11/18 (61.11%), Postives = 12/18 (66.67%), Query Frame = 1 
Query:  667 GTGPLENXXTHLADPWHN 720
            G GP+EN   HLADP  N
Sbjct:  236 GKGPIENLYDHLADPVAN 253
[Close]
BLAST of EY703166 vs. ExPASy Swiss-Prot
Match: LH15_EUGGR (Light-harvesting complex I LH35 proteins (Fragment) OS=Euglena gracilis PE=2 SV=1)

HSP 1 Score: 66.2402 bits (160), Expect = 7.452e-13
Identity = 51/148 (34.46%), Postives = 67/148 (45.27%), Query Frame = 3 
Query:  126 PAAANATSKYAMSADWMPGEPRPPYLDGSAPGDFGFDPLRLGEVPENLERYKESELIHCRWAMLAVPGILVPEALGLGNWVQAQEWAAVPGGQATYLGNPVPWGTLPTILVIEFLSIAFVEHQR-----SMEKDPEKKKYPGGAFDPL 554
            P A  AT+ +     W PG   P +L G  P D GFDPL +   P    R + SE+ H R +MLA+ G +VPE LG G W +              +GN V    L  IL+      A  E+ R     + +K    + YPG  FDPL
Sbjct:  148 PVAMFATTGHKDGV-WFPGAQPPAHLTGEYPADRGFDPLSVAADPTVYARMRVSEVFHARLSMLAIVGSIVPELLGKGAWFE--------------VGNSVDGIKLGFILM---AIAAPTEYWRGNGGFNWDKGTADRSYPG--FDPL 275

HSP 2 Score: 28.8758 bits (63), Expect = 7.452e-13
Identity = 14/31 (45.16%), Postives = 19/31 (61.29%), Query Frame = 2 
Query:  566 DPQKFE-EYKVKEIKNGRLALLAFVGFCVQH 655
            DP K   +Y  +EIKNGRLA+   +G   Q+
Sbjct:  273 DPLKLTTDYTKREIKNGRLAMTGLLGLTFQY 303
[Close]
BLAST of EY703166 vs. ExPASy Swiss-Prot
Match: LH18_EUGGR (Light-harvesting complex I LH38 proteins (Fragment) OS=Euglena gracilis PE=2 SV=1)

HSP 1 Score: 73.1738 bits (178), Expect = 2.369e-12
Identity = 41/91 (45.05%), Postives = 47/91 (51.65%), Query Frame = 3 
Query:  126 PAAANATSKYAMSAD---------WMPGEPRPPYLDGSAPGDFGFDPLRLGEVPENLERYKESELIHCRWAMLAVPGILVPEALGLGNWVQ 371
            P  AN T+  AM A          W P    P YL G A GD GFDP  L   P   ER + +E+ HCR +MLA+ G LVPE LG G W Q
Sbjct:  350 PIGANITTNLAMFASSGAKEERELWFPNIVPPRYLTGEAYGDKGFDPAGLAADPVTFERMQVAEVFHCRLSMLALVGCLVPELLGNGAWFQ 440
[Close]
BLAST of EY703166 vs. ExPASy Swiss-Prot
Match: CB2C_SOLLC (Chlorophyll a-b binding protein 1C, chloroplastic (Fragments) OS=Solanum lycopersicum GN=CAB1C PE=3 SV=1)

HSP 1 Score: 52.7582 bits (125), Expect = 6.782e-11
Identity = 25/41 (60.98%), Postives = 29/41 (70.73%), Query Frame = 2 
Query:  530 PRWCFRPLGLLQDPQKFEEYKVKEIKNGRLALLAFVGFCVQ 652
            P   F PLGL +DP+ F E KVKEIKNGRLA+ +  GF VQ
Sbjct:  190 PGGSFDPLGLAEDPEAFAELKVKEIKNGRLAMFSMFGFFVQ 230

HSP 2 Score: 28.8758 bits (63), Expect = 6.782e-11
Identity = 12/18 (66.67%), Postives = 13/18 (72.22%), Query Frame = 1 
Query:  667 GTGPLENXXTHLADPWHN 720
            G GPLEN   HLADP +N
Sbjct:  235 GKGPLENLADHLADPVNN 252

HSP 3 Score: 26.1794 bits (56), Expect = 6.782e-11
Identity = 9/10 (90.00%), Postives = 10/10 (100.00%), Query Frame = 3 
Query:  528 YPGGAFDPLG 557
            YPGG+FDPLG
Sbjct:  189 YPGGSFDPLG 198
[Close]
BLAST of EY703166 vs. ExPASy Swiss-Prot
Match: CB2A_SOLLC (Chlorophyll a-b binding protein 1A, chloroplastic (Fragments) OS=Solanum lycopersicum GN=CAB1A PE=3 SV=1)

HSP 1 Score: 52.7582 bits (125), Expect = 6.782e-11
Identity = 25/41 (60.98%), Postives = 29/41 (70.73%), Query Frame = 2 
Query:  530 PRWCFRPLGLLQDPQKFEEYKVKEIKNGRLALLAFVGFCVQ 652
            P   F PLGL +DP+ F E KVKEIKNGRLA+ +  GF VQ
Sbjct:  190 PGGSFDPLGLAEDPEAFAELKVKEIKNGRLAMFSMFGFFVQ 230

HSP 2 Score: 28.8758 bits (63), Expect = 6.782e-11
Identity = 12/18 (66.67%), Postives = 13/18 (72.22%), Query Frame = 1 
Query:  667 GTGPLENXXTHLADPWHN 720
            G GPLEN   HLADP +N
Sbjct:  235 GKGPLENLADHLADPVNN 252

HSP 3 Score: 26.1794 bits (56), Expect = 6.782e-11
Identity = 9/10 (90.00%), Postives = 10/10 (100.00%), Query Frame = 3 
Query:  528 YPGGAFDPLG 557
            YPGG+FDPLG
Sbjct:  189 YPGGSFDPLG 198
[Close]
BLAST of EY703166 vs. ExPASy Swiss-Prot
Match: CB2D_SOLLC (Chlorophyll a-b binding protein 1D (Fragment) OS=Solanum lycopersicum GN=CAB1D PE=3 SV=1)

HSP 1 Score: 52.7582 bits (125), Expect = 7.923e-11
Identity = 25/41 (60.98%), Postives = 29/41 (70.73%), Query Frame = 2 
Query:  530 PRWCFRPLGLLQDPQKFEEYKVKEIKNGRLALLAFVGFCVQ 652
            P   F PLGL +DP+ F E KVKEIKNGRLA+ +  GF VQ
Sbjct:   41 PGGSFDPLGLAEDPEAFAELKVKEIKNGRLAMFSMFGFFVQ 81

HSP 2 Score: 28.8758 bits (63), Expect = 7.923e-11
Identity = 12/18 (66.67%), Postives = 13/18 (72.22%), Query Frame = 1 
Query:  667 GTGPLENXXTHLADPWHN 720
            G GPLEN   HLADP +N
Sbjct:   86 GKGPLENLADHLADPVNN 103

HSP 3 Score: 26.1794 bits (56), Expect = 7.923e-11
Identity = 9/10 (90.00%), Postives = 10/10 (100.00%), Query Frame = 3 
Query:  528 YPGGAFDPLG 557
            YPGG+FDPLG
Sbjct:   40 YPGGSFDPLG 49
The following BLAST results are available for this feature:
BLAST of EY703166 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 80
Match NameE-valueIdentityDescription
CB4A_SOLLC3.785e-1639.31Chlorophyll a-b binding protein CP24 10A, chloropl... [more]
CB2_MALDO8.299e-1631.38Chlorophyll a-b binding protein AB10, chloroplasti... [more]
CB21_SILPR3.285e-1437.96Chlorophyll a-b binding protein, chloroplastic (Fr... [more]
CB4B_SOLLC1.640e-1334.91Chlorophyll a-b binding protein CP24 10B, chloropl... [more]
CB21_PINTH2.638e-1335.00Chlorophyll a-b binding protein type I, chloroplas... [more]
LH15_EUGGR7.452e-1334.46Light-harvesting complex I LH35 proteins (Fragment... [more]
LH18_EUGGR2.369e-1245.05Light-harvesting complex I LH38 proteins (Fragment... [more]
CB2C_SOLLC6.782e-1160.98Chlorophyll a-b binding protein 1C, chloroplastic ... [more]
CB2A_SOLLC6.782e-1160.98Chlorophyll a-b binding protein 1A, chloroplastic ... [more]
CB2D_SOLLC7.923e-1160.98Chlorophyll a-b binding protein 1D (Fragment) OS=S... [more]

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Properties
Property NameValue
Genbank descriptionCS00-C3-701-016-F04-CT.F Sweet orange fruit, development stadium (2 of 6) Citrus sinensis cDNA, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>EY703166 ID=EY703166; Name=EY703166; organism=Citrus sinensis; type=EST; length=913bp
CCACGCGTCCGCACAGCTCTCCCAAAATGGCTTCCAACACTCTGATGAGC
TGCGGCATCGCCACCACCGCTTTCCCTTCGGTCCTCTCATCTTCTAAATC
CAAATTCTTTACTGCAGTTTCACTTCCAGCTGCCGCCAATGCAACCTCGA
AATACGCCATGTCTGCTGACTGGATGCCCGGCGAGCCCCGGCCGCCTTAT
CTTGATGGTTCAGCCCCAGGGGACTTTGGATTTGACCCACTTCGGCTTGG
CGAAGTTCCTGAGAACCTTGAGAGATACAAGGAGTCTGAACTCATTCACT
GCAGATGGGCTATGCTTGCTGTTCCAGGGATCTTGGTGCCAGAGGCATTG
GGATTGGGCAACTGGGTGCAGGCGCAAGAGTGGGCAGCAGTTCCTGGAGG
ACAAGCCACATACTTAGGCAACCCTGTTCCATGGGGCACTCTCCCCACAA
TTTTGGTGATTGAATTCCTTTCCATTGCCTTCGTTGAGCACCAACGCAGC
ATGGAGAAAGACCCCGAGAAGAAGAAGTACCCCGGTGGTGCTTTCGACCC
CTTGGGCTTCTCCAAGACCCCCAGAAGTTCGAGGAATACAAAGTCAAGGA
GATAAAAAATGGGCGTTTGGCATTGTTGGCATTTGTGGGTTTCTGCGTGC
AACATCAGCATATCCGGGCACAGGACCATTGGAGAACTTNGNCACTCACT
TGGCTGATCCATGGCACAACACATTGGGGGACGTATTATCCCCAGAGGGA
TTGTGCCCTGAGAACTGCATTGTTAAATTATCCAGTCACTGTAAAATTCC
CTGNTAAAAGAGCCTTTATGAAACAGAATCGTATCTTTCTGACTGTAAGA
TTAGAAAGTAATGTGCCTCCATGACGTTCGTGCTAGAATCAATACTACTG
GGATTGCCGAAAA
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